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In vitro evaluation of coenzyme Q10 on primary fibroblast culture 体外评价辅酶Q10对原代成纤维细胞培养的影响
Pub Date : 2023-04-01 DOI: 10.22146/majkedgiind.80158
D. Herawati
Chronic inflammation in periodontitis results in continuous production of Reactive Oxygen Species (ROS), so the levels are excessive, causing destruction of the gingiva, periodontal ligament, and alveolar bone through a variety of mechanisms including DNA damage and the formation of proinflammatory cytokine. One way to prevent periodontal tissue damage caused by excessive ROS formation is by administering antioxidants. Coenzyme Q10 is a powerful antioxidant that is beneficial for inhibiting free radicals to prevent the progression of periodontal tissue destruction and accelerate healing processes. The purpose of this study was to evaluate the fibroblast proliferation of the combination of Coenzyme Q10 and vegetable glycerin compared to PerioQ. Materials used were made of original Coenzyme Q10 dissolved in glycerin that was prepared in a ratio of 2:8 and 1:9, and Perio Q as the control. Each group consisted of six samples (n = 6). Primary fibroblasts were derived from healthy gingival tissue. Observations on day-1, -3, and -5 using MTT assay at a wavelength of 550nm. Statistical analysis used a Two-Way ANOVA test followed by a Post Hoc test.  The experiment showed the absorbance values were high in all the groups, the highest value was on day 3, namely Coenzyme Q10 at a concentration of 2:8, followed by Coenzyme Q10 at a concentration of 1:9, and PerioQ. The statistical tests showed significant differences in the 3 groups (p < 0.05). It is concluded that Coenzyme Q10 in 1:9 and 2:8 concentrations were both as viable as Perio Q towards primary gingival fibroblast culture. 
牙周炎的慢性炎症导致活性氧(Reactive Oxygen Species, ROS)持续产生,水平过高,通过DNA损伤和促炎细胞因子的形成等多种机制对牙龈、牙周韧带和牙槽骨造成破坏。防止过度活性氧形成造成牙周组织损伤的一种方法是使用抗氧化剂。辅酶Q10是一种强大的抗氧化剂,有利于抑制自由基,防止牙周组织破坏的进展,加速愈合过程。本研究的目的是评价辅酶Q10和植物甘油组合与PerioQ相比对成纤维细胞增殖的影响。所用材料为原辅酶Q10溶于甘油,按2:8和1:9的比例配制,以Perio Q为对照。每组6个样本(n = 6)。原代成纤维细胞来源于健康牙龈组织。在第1、3、5天使用MTT法,波长为550nm。统计分析采用双向方差分析和事后检验。实验结果表明,各组吸光度值均较高,第3天吸光度值最高,为辅酶Q10浓度为2:8,其次为辅酶Q10浓度为1:9,最后为PerioQ。经统计学检验,3组间差异有统计学意义(p < 0.05)。由此可见,辅酶Q10在1:9和2:8浓度下对原代牙龈成纤维细胞的培养均具有与Q期相同的活性。
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引用次数: 1
A histological evaluation of cellular response on bone regeneration scaffold 骨再生支架细胞反应的组织学评价
Pub Date : 2023-04-01 DOI: 10.22146/majkedgiind.77449
Anne Handrini Dewi, Dyah Listyarifah, Retno Ardhani, J. Jansen
Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.
在临床使用之前,对新的植入材料进行生物相容性测试是必要的。骨支架材料还必须通过体外和体内生物相容性测试。本研究的目的是比较不同的染色方法在组织学准备,以评估材料的生物相容性组织。选取4月龄Wistar大鼠20只,雄性,体重约250 ~ 350 g,分为皮下植入组和骨植入组,分别为3、14、30 d。样品是一种以石膏和碳酸钙为基本成分的可生物降解材料。在乙二腈-o-四乙酸(EDTA)预脱钙后,用苏木精伊红(HE)、Mallory、甲苯胺蓝、抗酒石酸酸性磷酸酶多克隆抗体(TRAP)和Chromogen 3,3 - diaminobiidine (DAB)制备了多种方法的组织染色。使用光学显微镜(尼康eclipse E600)和OptiLab观察器,放大倍数从10X10到100X10进行观察。结果以不同染色方法生成的图像描述的形式显示。通常选择HE法染色,因为它既便宜又容易,但其他染色方法,如甲苯胺蓝、Mallory和TRAP,对比度更好。组织染色技术与免疫组织化学(DAB, VEGF)用于评估细胞和组织反应通过识别特定的蛋白质存在。
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引用次数: 0
In vitro study of the stretching time-associated force magnitude generated by NiTi and stainless-steel closed coil springs 镍钛和不锈钢封闭螺旋弹簧产生的拉伸时间相关力的体外研究
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.74970
Jennifer Xavier Ongko, M. Yusuf, Siti Bahirrah
Closed coil spring is an additional tool in a fixed-orthodontic treatment. NiTi is an orthodontic alloy that has shape memory and elasticity features, while stainless steel has properties of malleability, low friction between wire-bracket, as well as easy soldering and welding. Various properties of closed coil springs have been widely studied, but research on the stretching time remains unclear. This study aimed to determine the effect and differences in the stretching time for 7 days, 14 days, 21 days, and 28 days on the force magnitude of NiTi and stainless-steel (SS) closed coil spring. A 7 x 5 x 1 cm acrylic blocks with 1 cm acrylic posts were employed, and 25 mm closed coil springs were attached to the acrylic posts using modified T-sticks. The springs were immersed in an artificial saliva with pH of 6.75 for 7, 14, 21, and 28 days in an incubator at 37 °C. The container was removed from the incubator and the force was measured using a universal testing machine. Kolmogorov-Smirnoff test was carried out to analyze parametricdata, while independent T-test was used to determine the difference between each group. There was a significant effect of stretching time on the force magnitude of the SS closed coil spring in all the groups (p < 0.05). There was no significant effect of stretching time on the force of the NiTi closed coil spring on days 7, 14, and 21 (p > 0.05) but there was a significant effect on day 28 (p < 0.05). A significant effect between the ratio of the SS and NiTi closed coil spring (p < 0.05) was confirmed. Longer stretching time indicated a force loss in the SS closed-coil spring, while the NiTi spring had a more stable force which implies a formidable treatment-time for orthodontic treatment.
封闭线圈弹簧是固定正畸治疗的附加工具。NiTi是一种具有形状记忆和弹性特性的正畸合金,而不锈钢具有延展性、丝托间摩擦小、易钎焊和焊接等特性。闭式螺旋弹簧的各种性能已被广泛研究,但对其拉伸时间的研究尚不清楚。本研究旨在确定拉伸时间为7天、14天、21天和28天对NiTi和不锈钢(SS)封闭线圈弹簧的受力大小的影响和差异。采用7 × 5 × 1厘米的丙烯酸块和1厘米的丙烯酸柱子,并使用改良的t型棒将25毫米的封闭螺旋弹簧连接到丙烯酸柱子上。在37°C的培养箱中,将弹簧浸入pH为6.75的人工唾液中7、14、21和28天。将容器从培养箱中取出,用万能试验机测量力。参数资料分析采用Kolmogorov-Smirnoff检验,各组间差异采用独立t检验。拉伸时间对SS闭合线圈弹簧的受力大小有显著影响(p < 0.05)。拉伸时间在第7、14、21天对NiTi闭合线圈弹簧的受力无显著影响(p > 0.05),但在第28天有显著影响(p < 0.05)。SS与NiTi闭合线圈弹簧的比值有显著影响(p < 0.05)。拉伸时间越长,SS闭合线圈弹簧的力损失越大,而NiTi弹簧的力更稳定,正畸治疗时间较长。
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引用次数: 0
Effect of 4% and 15% moringa leaf extract gel on gingival wound healing in rats 4%和15%辣木叶提取物凝胶对大鼠牙龈创面愈合的影响
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.76416
Eka Pramudita Ramadhany, Gusti Agung, Dyah Ambarawati, Muhammad Rafif Musyaffa
Traumatic dental procedures such as incisions and gingival flaps are the leading causes of injury to the gingival structure. One of natural ingredients that can potentially accelerate the wound healing process is Moringa leaves Moringa oleifera). Moringa leaf has several active compounds, one of which is flavonoids, which can be an antiinflammatory and antibacterial agent, and increase collagen synthesis. A randomized posttest-only control-group design was used in this study. 48 wistar rats were categorized into four groups: CMC-Na gel, hyaluronic acid, 4% Moringa leaf extract gel, and 15% Moringa leaf extract gel. The samples were then euthanized on days 1, 3, 5, and 7. The two-way ANOVA test described significant differences (p < 0.05) for all the components of the observation (neutrophils, fibroblasts, angiogenesis, and epithelial thickness). The lowest mean number of neutrophils, the highest mean number of fibroblasts, and the highest mean of angiogenesis were found in the 15% Moringa extract group on the 7th day. The highest mean epithelial thickness was found in the use of 4% Moringa extract on the 5thday. The microscopic images showed that the treatment group gained more effective wound healing processes than the control group. The microscopic image showed that, in terms of neutrophils, fibroblasts, and angiogenesis, 15% Moringa extract was more effective for wound healing compared to 4% Moringa extract.
创伤性牙科手术,如切口和牙龈皮瓣是导致牙龈结构损伤的主要原因。辣木叶是一种可以加速伤口愈合过程的天然成分。辣木叶含有多种活性化合物,其中一种是类黄酮,它可以抗炎和抗菌,并增加胶原蛋白的合成。本研究采用随机后测对照组设计。48只wistar大鼠分为四组:CMC-Na凝胶组、透明质酸组、4%辣木叶提取物凝胶组、15%辣木叶提取物凝胶组。然后在第1、3、5和7天对这些样本实施安乐死。双向方差分析检验描述了观察的所有组成部分(中性粒细胞、成纤维细胞、血管生成和上皮厚度)的显著差异(p < 0.05)。15%辣木提取物组在第7天中性粒细胞的平均值最低,成纤维细胞的平均值最高,血管新生的平均值最高。平均上皮厚度以4%辣木提取物第5天最高。显微图像显示,治疗组比对照组获得了更有效的伤口愈合过程。显微镜图像显示,在中性粒细胞、成纤维细胞和血管生成方面,15%辣木提取物比4%辣木提取物对伤口愈合更有效。
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引用次数: 0
Differences in mandibular morphology between bruxism and non-bruxism patients based on the Levandoski analysis 基于Levandoski分析的磨牙患者与非磨牙患者下颌形态差异
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.76015
Aghitsna Aulia Aufa, R. Rikmasari, Daisy Wulansari
Bruxism is a condition that involves grinding and clenching which can place a large load on the mandible, so it can change the morphology of the mandible. Based on the Levandoski analysis, this study aimed to determine whether there are differences in the mandibular morphology between bruxism and non-bruxism patients. This study was a cross-sectional analytic study conducted on the panoramic radiographic samples of bruxism and non-bruxism patients with a total sample of 30 patients (n = 30) in each group, obtained from the radiographic archives at the Radiology Installation of RSGM Universitas Padjadjaran which have been confirmed as bruxism and non-bruxism patients. Measurements were carried out using ImageJ software based on the reference lines by Levandoski. The results of the study were analyzed using SPSS software by performing normality and homogeneity tests, followed by a statistically independent t-test or Mann-Whitney test. The results of the independent t-test showed that three lines had a p-value < 0.05, namely the line from the point gonion to the maxillary vertical line on the right side, the distance from the top point of the condyle to the maxillary interincisal point on the right side, and the distance from the top point of the condyle to the mandibular interincisal point on the right side. The Mann-Whitney test results showed that two lines had a p-value < 0.05, namely the maxillary vertical midline and the distance from the condyle to the left maxillary vertical line. The other line had a p-value> 0.05. There were differences in the mandibular morphology between bruxism and non-bruxism patients based on the Levandoski analysis, namely the length of the maxillary vertical midline, the distance from the condyle top point to the maxillary vertical midline, the distance from the point gonion to the maxillary vertical midline, the distance from the top point of the condyle to the maxillary interincisal point on the left side, and the distance from the top point of the condyle to the mandibular interincisal point on the left side. On the other four lines, there was no significant difference between bruxism and non-bruxism patients.
磨牙症是一种磨牙和咬牙的情况,它会给下颌骨带来很大的负荷,所以它会改变下颌骨的形态。在Levandoski分析的基础上,本研究旨在确定磨牙患者与非磨牙患者的下颌形态是否存在差异。本研究是对磨牙症和非磨牙症患者的全景x线片样本进行横断面分析研究,每组30例(n = 30),样本来自于Padjadjaran Universitas RSGM放射学装置的放射档案,已确认为磨牙症和非磨牙症患者。基于Levandoski的参考线,使用ImageJ软件进行测量。采用SPSS软件对研究结果进行正态性和齐性检验,并进行统计学独立t检验或Mann-Whitney检验。独立t检验结果显示,有三条线的p值均< 0.05,分别为点阴至右侧上颌垂直线、髁顶至右侧上颌内尖点的距离、髁顶至右侧下颌骨内尖点的距离。Mann-Whitney检验结果显示,上颌垂直中线和髁突到左上颌垂线的距离有两条线p值< 0.05。另一条线的p值> 0.05。根据Levandoski分析,磨牙患者与非磨牙患者的下颌形态存在差异,即上颌垂直中线的长度,髁突顶点到上颌垂直中线的距离,点位到上颌垂直中线的距离,左侧髁突顶点到上颌内尖点的距离,以及从髁突顶端到左侧下颌骨内尖的距离。在其他四条线上,磨牙患者和非磨牙患者之间没有显著差异。
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引用次数: 1
Treatment of temporomandibular joint trauma following odontectomy 牙切除后颞下颌关节创伤的治疗
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.78415
Intan Ruspita, H. Dipoyono
Temporomandibular Joint Disorder (TMD) is a non-dental musculoskeletal disorder that has a complex and multifactorial etiology. The most frequent symptoms are pain in the masticatory muscles, in front of the ears, and in the temporomandibular joint (TMJ); this disorder can also be accompanied by clicking sounds and locking. This study aimed to present a case report of temporomandibular joint trauma following odontectomy. A 22-year-old patient came with some complaints, namely pain in the TMJ, a clicking sound when opening and closing the mouth, and limited mouth opening. The symptoms started when the patient underwent odontectomy on all the four third molars, causing tightness around the jaw, pain, and clicking. However, she did not seek any treatment for these conditions until the pain became worse and disturbed her activities. The patient had to deal with limited mouth opening and severe migraines up to twice a day. During an examination, the patient was able to open her mouth without pain only 25 mm in width, with a maximum mouth opening of 35 mm. The therapy was done by constructing a 4-mm stabilization splint and educating the patient on how to reduce the pain. In fact, 2 months after the treatment and the use of stabilization splints, her complaints of TMJ pain and clicking sounds decreased, her mouth opening increased, and the migraines never came back. In this case, non-invasive treatment as the first-line therapy in patients with disc displacement with reduction and arthralgia can reduce the joint pain symptoms, and some of the symptoms have even disappeared.
颞下颌关节紊乱(TMD)是一种非牙齿肌肉骨骼紊乱,具有复杂的多因素病因。最常见的症状是咀嚼肌疼痛、耳前疼痛和颞下颌关节疼痛;这种障碍也可能伴随着咔哒声和锁定声。本研究的目的是提出一个病例报告的颞下颌关节创伤后,牙切除。一名22岁的患者出现了一些症状,即颞下颌关节疼痛、张嘴和闭口时发出咔嗒声、张嘴受限。患者在进行了全部四颗第三磨牙的牙齿切除手术后,出现了下颌周围紧绷、疼痛和咔嗒声。然而,她没有寻求任何治疗,直到疼痛变得更严重,并干扰了她的活动。病人不得不处理有限的张嘴和严重的偏头痛,每天两次。在一次检查中,患者能够无痛地张开她的嘴,宽度只有25毫米,最大开口为35毫米。治疗是通过构建一个4毫米稳定夹板和教育患者如何减轻疼痛来完成的。事实上,在治疗和使用稳定夹板2个月后,她对TMJ疼痛和咔嗒声的抱怨减少了,她的张嘴增加了,偏头痛再也没有复发。在这种情况下,无创治疗作为椎间盘移位伴复位和关节痛患者的一线治疗方法,可减轻关节疼痛症状,部分症状甚至消失。
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引用次数: 0
Knowledge of pregnant women about risk factor of cleft lip and palate at Puskesmas Mutiara, Asahan, Indonesia 印度尼西亚阿萨罕Puskesmas Mutiara孕妇对唇腭裂危险因素的了解情况
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.71456
Hendry Rusdy, Rahmi Syaflida, O. A. Hanafiah, Jemima Ratnaningtyas
Cleft is a congenital abnormal space or gap in the upper lip, alveolus, or palate. This congenital abnormality occurs between the 5th and 10th weeks of pregnancy. Clefts are divided into cleft lip, cleft palate, as well as cleft lip and palate. Cleft lip and palate are caused by the interaction of individual genes with certain environmental factors. Mothers’ knowledge about risk factor of cleft lip and palate may promote better health-related behavior in their pregnancy by increasing the understanding about the risk factor. Unfortunately, until now there is still limited data about this. The purpose of this study was to determine the knowledge of pregnant women about risk factor of cleft lip and palate. This was a descriptive study that used the survey method. This study was conducted using a questionnaire distributed to 67 pregnant women (n = 67). The questionnaire consisted of 13 validated questions. The results of this study found that 10.4% of the respondents had good knowledge, 32.8% of the respondents had moderate knowledge and 56.7% of the respondents had poor knowledge. The overall knowledge of the pregnant women about risk factor of cleft lip and palate at Puskesmas Mutiara Asahan fell in the low category.
唇裂是先天性上唇、牙槽或上颚的异常空间或间隙。这种先天性畸形发生在怀孕的第5周到第10周之间。唇裂分为唇裂、腭裂以及唇腭裂。唇腭裂是个体基因与环境因素共同作用的结果。母亲对唇腭裂危险因素的了解可以通过增加对危险因素的了解来促进怀孕期间更好的健康相关行为。不幸的是,到目前为止,关于这方面的数据仍然有限。本研究的目的是了解孕妇对唇腭裂危险因素的了解程度。这是一项使用调查方法的描述性研究。本研究对67名孕妇(n = 67)进行问卷调查。问卷由13个有效问题组成。本研究结果发现,10.4%的被调查者知识较好,32.8%的被调查者知识一般,56.7%的被调查者知识较差。孕妇对唇腭裂危险因素的总体了解程度处于低水平。
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引用次数: 0
Relationship between peatland water use and periodontal disease in Daha Selatan Subdistrict 大哈塞拉坦街道泥炭地用水与牙周病的关系
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.76843
Antung Nurul Hidayah, Beta Widya Oktiani, A. Azizah
Periodontal disease is one of the dental and oral health problems in Hulu Sungai Selatan Regency with a prevalence of gingivitis of 24.58% and periodontitis of 10.83%. One of the risk factors for periodontal disease is the use of peatland water. Peatland water has a low pH which can affect the colony of anaerobic bacteria in the oral cavity of the water users. Bacteria that cause periodontal disease are able to develop and survive in acidic conditions. This study aimed to analyze the relationship between peatland water use and periodontal disease in Daha SelatanSubdistrict. This was analytical research with a cross-sectional approach. The sampling technique used a simple random sampling technique with a total sample of 100 people (n = 100) who were residents aged 35-44 years in Daha Selatan Subdistrict. The results showed that the participants with high use of peatland water showedthe highest percentage (39%). In addition, there were 61% respondents who suffered from periodontal disease. ChiSquare test obtained a significance value of 0.022 (p < 0.05), showing that there was a relationship between peatland water use and periodontal disease. The risk of periodontal disease in the respondents who used peatland water in the high category was 4.167 times greater than in the respondents who did not use peatland water. It is essential to educate the general public about the effects that using peatland water can have on dental and oral health because there is a high prevalence of periodontal disease, which is caused by the widespread use of peatland water.
牙周病是牙龈炎的患病率为24.58%,牙周炎患病率为10.83%。使用泥炭地水是牙周病的危险因素之一。泥炭地的水pH值较低,会影响使用者口腔内厌氧菌的菌落。引起牙周病的细菌能够在酸性条件下生长和生存。本研究旨在分析大哈塞拉坦街道泥炭地用水与牙周病的关系。这是一项采用横断面方法的分析研究。抽样方法采用简单随机抽样方法,共抽样100人(n = 100),年龄在35-44岁之间。结果表明,使用泥炭地水最多的参与者所占比例最高(39%)。此外,61%的受访者患有牙周病。ChiSquare检验的显著性值为0.022 (p < 0.05),表明泥炭地用水与牙周病之间存在相关性。使用高类别泥炭地水的受访者患牙周病的风险是未使用泥炭地水的受访者的4.167倍。必须向公众宣传使用泥炭地水可能对牙齿和口腔健康产生的影响,因为由于广泛使用泥炭地水,牙周病的发病率很高。
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引用次数: 0
The relationship between bizygomatic width and mesiodistal width of six maxillary anterior teeth in a Javanese population 爪哇人群6颗上颌前牙颧宽与近远端宽度的关系
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.79146
Murti Indrastuti, Sri Budi Barunawati, Dian Samuel Hasudungan
The selection of the mesiodistal width of maxillary anterior teeth is the key to success in prosthodontic treatment. Determining anterior teeth width when there is no existing pre-extraction record can be done through bizygomatic width measurement. The purpose of this study was to look at the relationship between bizygomatic width and mesiodistal width of six maxillary anterior teeth in the Javanese population. This study used a cross-sectional design and was conducted on 32 Javanese subjects who met the following criteria: being 18-25 years of age, having Angle class 1 malocclusion, having complete maxillary anterior permanent teeth, not using dentures, currently not undergoing any orthodontic treatment, and having no abnormalities found in the facial area. The bizygomatic width was measured with a digital caliper with an accuracy of 0.01 mm from the marked right and left bizygomatic points. The mesiodistal width of the six maxillary anterior teeth was measured using dental floss looped from the distal left canine to the right canine. Dental floss marked with a pen was stretched horizontally on paper and then measured with a digital caliper with an accuracy of 0.01 mm. The measurement was done in triplicate. Data were analyzed using Pearson’s correlation and the simple linear regression test. This study’s analysis revealed a strong and positive correlation (r = 0.538 and p < 0.05) between the bizygomatic width and mesiodistal width of the maxillary anterior teeth. The result of the simple linear regression test showed a significant result (p < 0.05). It can be concluded that there was a relationship between the bizygomatic width and the mesiodistal width of the six maxillary anterior teeth in the Javanese population. The mesiodistal width of the six maxillary anterior teeth in the Javanese population can be measured by multiplying the bizygomatic width by the multiplier factor, i.e., 0.427.
上颌前牙近远端宽度的选择是修复治疗成功的关键。在没有拔牙前记录的情况下,可以通过测量双颧宽度来确定前牙宽度。本研究的目的是观察爪哇人群六颗上颌前牙的颧宽与中远端宽度之间的关系。本研究采用横断面设计,选取32名爪哇人,符合以下标准:年龄18-25岁,有Angle 1类错颌,上颌前恒牙完整,未使用假牙,目前未接受任何正畸治疗,面部未发现异常。用数字卡尺从标记的左右菱形点测量菱形宽度,精度为0.01 mm。用牙线环从左犬齿到右犬齿测量六颗上颌前牙的中远端宽度。用钢笔标记的牙线在纸上水平拉伸,然后用数字卡尺测量,精度为0.01毫米。测量一式三份。数据分析采用Pearson相关检验和简单线性回归检验。本研究结果显示,下颌颧宽与上颌前牙近远端宽度呈正相关(r = 0.538, p < 0.05)。简单线性回归检验结果有显著性差异(p < 0.05)。结果表明爪哇人群6颗上颌前牙的颧宽与中远端宽度之间存在一定的关系。爪哇人群6颗上颌前牙的中远端宽度可以用双颧宽度乘以乘数因子来测量,即0.427。
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引用次数: 0
Differences in Candida Albicans hydrophobicity and biofilm formation between thermoplastic nylon and polyether-ether-ketone denture base materials 热塑性尼龙与聚醚醚酮义齿基托材料白色念珠菌疏水性及生物膜形成的差异
Pub Date : 2023-01-06 DOI: 10.22146/majkedgiind.79300
Titik Ismiyati
Polyetheretherketone (PEEK) and thermoplastics nylon are extensively composed as an alternative material for removable denture base. The primary difference between the two materials is associated with water absorption properties. The two materials interact with saliva and microorganisms during application in the oral cavity. Candida is predominantly reported in the cavity. The microorganism is distinguished by its hydrophobicity and ability to form biofilms. The objective of this study was to determine the differences between thermoplastic nylon and polyether-ether-ketone as removable denture materials in terms of hydrophobicity and Candida albicans biofilms. It is a laboratory experiment using 20 5 x 2 mm samples for the hydrophobicity test and Candida albicans biofilm. The hydrophobicity test was performed by determining the contact angle with a Goniometer and the WinDrop++ software. The biofilm test samples were divided into 4 groups, encompassing PEG 5% (negative control), PEEK treatment, thermoplastics nylon, and 0.12% chlorhexidine (control positive). The microdilution method was employed to examine biofilm formation. The results of the study were interpreted, and the data were analyzed by employing the t test. The research discovered significant differences in biofilm formation, large contact angle on PEEK and thermoplastics nylon (p < 0.05). Biofilm formation on thermoplastics is significantly larger than PEEK (p < 0.05). In conclusion, the contact angles of PEEK and thermoplastic nylon are incorporated in the partially wetted. Polyetheretherketone is involved in the non-producers of biofilms while thermoplastic nylon is categorized in of weak biofilm formers.
聚醚醚酮(PEEK)和热塑性尼龙广泛组成作为可移动义齿基托的替代材料。这两种材料的主要区别在于吸水性能。这两种材料在口腔应用过程中与唾液和微生物相互作用。念珠菌主要见于口腔。这种微生物的特点是它的疏水性和形成生物膜的能力。本研究的目的是确定热塑性尼龙和聚醚醚酮作为活动义齿材料在疏水性和白色念珠菌生物膜方面的差异。这是一个实验室实验,使用205 × 2mm样品进行疏水性测试和白色念珠菌生物膜。疏水性试验采用测角仪和windrop++软件测定接触角。生物膜试验样品分为4组,分别为5% PEG(阴性对照)、PEEK处理、热塑性尼龙和0.12%氯己定(阳性对照)。采用微量稀释法检测生物膜的形成。对研究结果进行解释,采用t检验对数据进行分析。研究发现PEEK与热塑性尼龙在生物膜形成、大接触角方面存在显著差异(p < 0.05)。热塑性塑料的生物膜形成量显著大于PEEK (p < 0.05)。综上所述,PEEK和热塑性尼龙的接触角在部分润湿中被纳入。聚醚醚酮涉及非生物膜生产者,而热塑性尼龙被归类为弱生物膜形成者。
{"title":"Differences in Candida Albicans hydrophobicity and biofilm formation between thermoplastic nylon and polyether-ether-ketone denture base materials","authors":"Titik Ismiyati","doi":"10.22146/majkedgiind.79300","DOIUrl":"https://doi.org/10.22146/majkedgiind.79300","url":null,"abstract":"Polyetheretherketone (PEEK) and thermoplastics nylon are extensively composed as an alternative material for removable denture base. The primary difference between the two materials is associated with water absorption properties. The two materials interact with saliva and microorganisms during application in the oral cavity. Candida is predominantly reported in the cavity. The microorganism is distinguished by its hydrophobicity and ability to form biofilms. The objective of this study was to determine the differences between thermoplastic nylon and polyether-ether-ketone as removable denture materials in terms of hydrophobicity and Candida albicans biofilms. It is a laboratory experiment using 20 5 x 2 mm samples for the hydrophobicity test and Candida albicans biofilm. The hydrophobicity test was performed by determining the contact angle with a Goniometer and the WinDrop++ software. The biofilm test samples were divided into 4 groups, encompassing PEG 5% (negative control), PEEK treatment, thermoplastics nylon, and 0.12% chlorhexidine (control positive). The microdilution method was employed to examine biofilm formation. The results of the study were interpreted, and the data were analyzed by employing the t test. The research discovered significant differences in biofilm formation, large contact angle on PEEK and thermoplastics nylon (p < 0.05). Biofilm formation on thermoplastics is significantly larger than PEEK (p < 0.05). In conclusion, the contact angles of PEEK and thermoplastic nylon are incorporated in the partially wetted. Polyetheretherketone is involved in the non-producers of biofilms while thermoplastic nylon is categorized in of weak biofilm formers.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76505277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Majalah Kedokteran Gigi Indonesia
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