Pub Date : 2022-08-09DOI: 10.22146/majkedgiind.63382
A. Antony, H. Dipoyono, Titik Ismiyati
Acrylic resin is the most common fabricated material for denture bases. ZrO2 nanoparticles can be used as filler to strengthen the physical properties and inhibit the adhesion of acrylic resins. This study aimed to examine the growth of Candida albicans and porosity in reinforced acrylic resin with Zirconium Dioxide (ZrO2) nanoparticles at 2.5% and 5% a concentration targeted for denture bases application. A laboratory experiment study included twenty-seven disc-shaped samples which were divided into three groups: group I acrylic resin without ZrO2 nanoparticles (control), group II acrylic resin with 2.5% ZrO2 nanoparticles, and group III with 5% ZrO2 nanoparticle acrylic resin. Samples were collected by heat polymerization while porosity observations were done using primo zeizs starr (Nikon YS100) microscope at 100x magnification. Dilution test was implemented to assess Candida albican growth. One-way ANOVA and Post Hoc LSD test statistical analysis were performed to evaluate the data (p<0.05). The results indicated that the mean porosity values in the control group of 2.5% and 5% were 37.4 ± 3.5, 15.8 ± 3.3, 8.0 ± 2.0 respectively while the attachment of C. albicans was 0.832 ± 0.083, 0.536 ± 0.098, 0.218 ± 0.083. One-way ANOVA confirmed a significant effect (p<0.05). Similarly, Post Hoc LSD test reported significant difference (p<0.05). The results showed that acrylic resin reinforced with ZrO2 nanoparticles for heat-polymerized denture base effectively reduced porosity and C. albicans adhesion. Five percent of ZrO2 nanoparticle concentration presented greater porosity reduction compared to 2.5% ZrO2 and non-reinforced acrylic resin. Acrylic resin reinforced with ZrO2 nanoparticles in 2.5% and 5% concentration is fungistatic.
丙烯酸树脂是义齿基托最常用的制造材料。ZrO2纳米颗粒可以作为填料增强丙烯酸树脂的物理性能，抑制丙烯酸树脂的粘附。本研究旨在研究二氧化锆纳米颗粒在2.5%和5%浓度下用于义齿基托的增强丙烯酸树脂中白色念珠菌的生长和孔隙率。将27个圆盘状样品分为3组:不含ZrO2纳米颗粒的丙烯酸树脂组(对照)、含2.5% ZrO2纳米颗粒的丙烯酸树脂组和含5% ZrO2纳米颗粒的丙烯酸树脂组。样品采用热聚合法收集，孔隙率观察采用100倍放大的primo zeizs starr (Nikon YS100)显微镜。稀释试验评估白色念珠菌的生长情况。采用单因素方差分析和Post Hoc LSD检验进行统计分析(p<0.05)。结果表明，2.5%和5%对照组的平均孔隙度分别为37.4±3.5、15.8±3.3、8.0±2.0，白色念珠菌的附着度分别为0.832±0.083、0.536±0.098、0.218±0.083。单因素方差分析证实了显著影响(p<0.05)。同样，Post Hoc LSD检验报告有显著性差异(p<0.05)。结果表明，ZrO2纳米颗粒增强丙烯酸树脂用于热聚合义齿基托可有效降低气孔率和白色念珠菌粘附。与2.5%的ZrO2和未增强的丙烯酸树脂相比，5%的ZrO2纳米颗粒的孔隙率降低幅度更大。2.5%和5%浓度的ZrO2纳米粒子增强丙烯酸树脂具有抑菌作用。
{"title":"The effect of adding zirconium dioxide nanoparticle to acrylic denture base on porosity and candida albicans adhesion","authors":"A. Antony, H. Dipoyono, Titik Ismiyati","doi":"10.22146/majkedgiind.63382","DOIUrl":"https://doi.org/10.22146/majkedgiind.63382","url":null,"abstract":"Acrylic resin is the most common fabricated material for denture bases. ZrO2 nanoparticles can be used as filler to strengthen the physical properties and inhibit the adhesion of acrylic resins. This study aimed to examine the growth of Candida albicans and porosity in reinforced acrylic resin with Zirconium Dioxide (ZrO2) nanoparticles at 2.5% and 5% a concentration targeted for denture bases application. A laboratory experiment study included twenty-seven disc-shaped samples which were divided into three groups: group I acrylic resin without ZrO2 nanoparticles (control), group II acrylic resin with 2.5% ZrO2 nanoparticles, and group III with 5% ZrO2 nanoparticle acrylic resin. Samples were collected by heat polymerization while porosity observations were done using primo zeizs starr (Nikon YS100) microscope at 100x magnification. Dilution test was implemented to assess Candida albican growth. One-way ANOVA and Post Hoc LSD test statistical analysis were performed to evaluate the data (p<0.05). The results indicated that the mean porosity values in the control group of 2.5% and 5% were 37.4 ± 3.5, 15.8 ± 3.3, 8.0 ± 2.0 respectively while the attachment of C. albicans was 0.832 ± 0.083, 0.536 ± 0.098, 0.218 ± 0.083. One-way ANOVA confirmed a significant effect (p<0.05). Similarly, Post Hoc LSD test reported significant difference (p<0.05). The results showed that acrylic resin reinforced with ZrO2 nanoparticles for heat-polymerized denture base effectively reduced porosity and C. albicans adhesion. Five percent of ZrO2 nanoparticle concentration presented greater porosity reduction compared to 2.5% ZrO2 and non-reinforced acrylic resin. Acrylic resin reinforced with ZrO2 nanoparticles in 2.5% and 5% concentration is fungistatic.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74742636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-09DOI: 10.22146/majkedgiind.62691
Astriani Amanda, D. N. Ratih, Ema Mulyawati
Sisal nanofiber can be used as an additional filler to increase the adhesion strength of the resin epoxy sealer. The aim of this study was to observe the effect of sisal nanofiber addition to epoxy resin sealer on push-out bond strength of root canal obturation material against the root canals. Twenty-five mandibular premolars (n= 25) were prepared until file F3 and obturated using 0%, 0.25%, 0.5%, 0.75%, and 1% sisal nanofiber sealer. After the specimens were stored at incubator, they were horizontally sectioned at apical third. Root sections were tested with push-out technique, and observed under a stereo microscope to determine the failure type. Data were analyzed with one-way ANOVA and LSD pos hoc at 95% confidence level (α=0.05). A one-way ANOVA test revealed that the addition of sisal nanofiber in epoxy resin sealer had a significant effect on the push out bond strength of the obturation material against the root canals. The failure type was observed predominantly in the cohesive type and the lowest in the adhesive type. The addition of sisal nanofiber to the epoxy resin sealer could increase the push-out bond strength of the obturation material against the root canals. Epoxy resin sealers with the addition of sisal nanofiber at a concentration group of 0.75% resulted in the highest push-out strength of root canal obturation materials followed by 0.5% concentration group.
{"title":"The effect of sisal (Agave Sisalana) nanofiber in epoxy resin sealer on root canal obturation’s push-out bond strength","authors":"Astriani Amanda, D. N. Ratih, Ema Mulyawati","doi":"10.22146/majkedgiind.62691","DOIUrl":"https://doi.org/10.22146/majkedgiind.62691","url":null,"abstract":"Sisal nanofiber can be used as an additional filler to increase the adhesion strength of the resin epoxy sealer. The aim of this study was to observe the effect of sisal nanofiber addition to epoxy resin sealer on push-out bond strength of root canal obturation material against the root canals. Twenty-five mandibular premolars (n= 25) were prepared until file F3 and obturated using 0%, 0.25%, 0.5%, 0.75%, and 1% sisal nanofiber sealer. After the specimens were stored at incubator, they were horizontally sectioned at apical third. Root sections were tested with push-out technique, and observed under a stereo microscope to determine the failure type. Data were analyzed with one-way ANOVA and LSD pos hoc at 95% confidence level (α=0.05). A one-way ANOVA test revealed that the addition of sisal nanofiber in epoxy resin sealer had a significant effect on the push out bond strength of the obturation material against the root canals. The failure type was observed predominantly in the cohesive type and the lowest in the adhesive type. The addition of sisal nanofiber to the epoxy resin sealer could increase the push-out bond strength of the obturation material against the root canals. Epoxy resin sealers with the addition of sisal nanofiber at a concentration group of 0.75% resulted in the highest push-out strength of root canal obturation materials followed by 0.5% concentration group.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76552018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-09DOI: 10.22146/majkedgiind.62867
Heny Retno Mayangsari, Tunjung Nugraheni, Ema Mulyawati
Sealer is one of root canal filler materials which has been developed and has an antibacterial agent to keep root canal sterile during and after an obturation process. This study aimed to find out the effect of sisal nanofiber addition to resin epoxy-based sealer on the antibacterial power against Enterococccus faecalis (E. faecalis) which is known as adaptive and potent bacteria which can be both aerobic and anaerobic. Sisal fibers were processed through many stages to make it nano sized (scouring, bleaching, neutralization, ultrasonification, and freeze-drying). Once nano-sized sisal fibers had been obtained, they were then mixed with sealer powder (AH26) in different concentrations: powder 0%, 0.25%, 0.5%, 0.75% and 1%. These concentrations were chosen based on preliminary research for reasonable contact angle measurement of sisal-sealer mixture. Antibacterial effect was examined using the diffusion method, each concentration was tested in 5 petri dishes which were planted with 1.5 X 108 CFU/ml E.faecalis bacteria. Each dish consisted of 5 holes (6 mm in diameter), each hole represented each concentration of nano sisal and sealer which were mixed until homogenous for 3 minutes before added to each hole. The dishes were then incubated for 48 hours at 37 °C. Inhibitory zones were measured, and analyzed using one-way ANOVA. The one-way ANOVA result showed that p=0.502 (p>0.05), meaning that the sisal nanofiber addition to epoxy resin-based sealer had no effect on E.faecalis inhibition. Thus, there was no effect of sisal nanofiber addition to epoxy resin-based sealer on E. faecalis.
{"title":"Effects of sisal nanofiber addition to epoxy resin-based sealer on its antibacterial power against Enterococcus faecalis","authors":"Heny Retno Mayangsari, Tunjung Nugraheni, Ema Mulyawati","doi":"10.22146/majkedgiind.62867","DOIUrl":"https://doi.org/10.22146/majkedgiind.62867","url":null,"abstract":"Sealer is one of root canal filler materials which has been developed and has an antibacterial agent to keep root canal sterile during and after an obturation process. This study aimed to find out the effect of sisal nanofiber addition to resin epoxy-based sealer on the antibacterial power against Enterococccus faecalis (E. faecalis) which is known as adaptive and potent bacteria which can be both aerobic and anaerobic. Sisal fibers were processed through many stages to make it nano sized (scouring, bleaching, neutralization, ultrasonification, and freeze-drying). Once nano-sized sisal fibers had been obtained, they were then mixed with sealer powder (AH26) in different concentrations: powder 0%, 0.25%, 0.5%, 0.75% and 1%. These concentrations were chosen based on preliminary research for reasonable contact angle measurement of sisal-sealer mixture. Antibacterial effect was examined using the diffusion method, each concentration was tested in 5 petri dishes which were planted with 1.5 X 108 CFU/ml E.faecalis bacteria. Each dish consisted of 5 holes (6 mm in diameter), each hole represented each concentration of nano sisal and sealer which were mixed until homogenous for 3 minutes before added to each hole. The dishes were then incubated for 48 hours at 37 °C. Inhibitory zones were measured, and analyzed using one-way ANOVA. The one-way ANOVA result showed that p=0.502 (p>0.05), meaning that the sisal nanofiber addition to epoxy resin-based sealer had no effect on E.faecalis inhibition. Thus, there was no effect of sisal nanofiber addition to epoxy resin-based sealer on E. faecalis.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90071064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.53329
Dewi Arsih Sulistiani, Widjijono Widjijono, R. Dharmastiti
Cobalt chromium (CoCr) alloys are utilized to make dental prosthesis. Casting CoCr alloys is a common operation in dentistry laboratories due to its low cost. Casting surplus (metal remaining in the sprue and crucible former) is frequently reused by dental laboratories to reduce and recycle metal waste. However, the quality and safety of these recast alloys require further information. Microbial attachment to the surface of metal prostheses may affect its quality and safety. Biofilm formation on metal surface can cause biocorrosion and secondary infection. The effects of different proportions of recast Cobalt Chromium alloy on the bacterial adhesion are not clear. The purpose of this study was to investigate how recasting affects the Streptococcus mutans (S. mutans) adhesion. Twenty disk-shaped specimens (n= 20, allocated for 5 groups) were prepared from CoCr alloys (Remanium GM; Dentaurum) with different proportions (100% new alloy, 25% recast alloy, 50% recast alloy, 75% recast alloy, and 100% recast alloy). After the immersion of the specimens in bacterial suspension for 24 hours, the number of bacteria that adhere to the specimen’s surface was counted using Colony Forming Units. Data were analyzed with one-way ANOVA (α = 0.05). The bacterial adhesion was significantly affected by the recast alloys (p < 0.05). An increased proportion of CoCr recast alloys showed an increase in S. mutans adhesion to the specimen surface.
{"title":"Bacterial adhesion of Streptococcus mutans to cobalt chromium recast alloys","authors":"Dewi Arsih Sulistiani, Widjijono Widjijono, R. Dharmastiti","doi":"10.22146/majkedgiind.53329","DOIUrl":"https://doi.org/10.22146/majkedgiind.53329","url":null,"abstract":"Cobalt chromium (CoCr) alloys are utilized to make dental prosthesis. Casting CoCr alloys is a common operation in dentistry laboratories due to its low cost. Casting surplus (metal remaining in the sprue and crucible former) is frequently reused by dental laboratories to reduce and recycle metal waste. However, the quality and safety of these recast alloys require further information. Microbial attachment to the surface of metal prostheses may affect its quality and safety. Biofilm formation on metal surface can cause biocorrosion and secondary infection. The effects of different proportions of recast Cobalt Chromium alloy on the bacterial adhesion are not clear. The purpose of this study was to investigate how recasting affects the Streptococcus mutans (S. mutans) adhesion. Twenty disk-shaped specimens (n= 20, allocated for 5 groups) were prepared from CoCr alloys (Remanium GM; Dentaurum) with different proportions (100% new alloy, 25% recast alloy, 50% recast alloy, 75% recast alloy, and 100% recast alloy). After the immersion of the specimens in bacterial suspension for 24 hours, the number of bacteria that adhere to the specimen’s surface was counted using Colony Forming Units. Data were analyzed with one-way ANOVA (α = 0.05). The bacterial adhesion was significantly affected by the recast alloys (p < 0.05). An increased proportion of CoCr recast alloys showed an increase in S. mutans adhesion to the specimen surface.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88158936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.66092
R. T. E. Untara, Widjijono Widjijono, W. Asmara, D. N. Ratih
Vertical root fractures of intentional replantation need a material capable of binding the fragments of the fracture line tightly and encouraging the regeneration of periradicular tissue. One of the indicators that regeneration of periradicular tissue takes place is epithelial thickness. This study aimed to investigate the effect of mineral trioxide aggregate (MTA) and self-adhesive resin cement as the adhesive materials in sealing vertical root fractures of intentional replantation on the epithelial thickness of periradicular tissue. This study used 27 male New Zealand rabbits with the age range of 8-12 weeks. The mandibular incisor was extracted, and the tooth was cut from the cervical border to the 2/3 apical third. The samples were assigned randomly into three groups of 9 each, namely Group 1 with no application of any material in the fracture line (control group), Group 2 with MTA, and Group 3 with self-adhesive resin cement. All the teeth in all the groups were then inserted back into the socket. Each group was further divided into three subgroups based on the observation time, namely days 7, 14, and 21. Histological observations of the epithelial thickness were carried out under the light microscope (400x magnification). Data were analyzed using a two-way ANOVA and LSD post hoc test with a confidence level of 95%. The two-way ANOVA analysis showed that the materials used for sealing the fracture lines had a significant effect on the epithelial thickness (p<0.05), while the observation time did not affect the epithelial thickness (p>0.05). No interaction occurred between the material used and observation times (p>0.05). It can be concluded that MTA generated a greater epithelial thickness of periradicular tissue compared than self-adhesive resin cement in sealing vertical root fractures of intentional replantation.
{"title":"Effect of two different materials in sealing vertical root fractures of intentional replantation on epithelial thickness of periradicular tissue","authors":"R. T. E. Untara, Widjijono Widjijono, W. Asmara, D. N. Ratih","doi":"10.22146/majkedgiind.66092","DOIUrl":"https://doi.org/10.22146/majkedgiind.66092","url":null,"abstract":"Vertical root fractures of intentional replantation need a material capable of binding the fragments of the fracture line tightly and encouraging the regeneration of periradicular tissue. One of the indicators that regeneration of periradicular tissue takes place is epithelial thickness. This study aimed to investigate the effect of mineral trioxide aggregate (MTA) and self-adhesive resin cement as the adhesive materials in sealing vertical root fractures of intentional replantation on the epithelial thickness of periradicular tissue. This study used 27 male New Zealand rabbits with the age range of 8-12 weeks. The mandibular incisor was extracted, and the tooth was cut from the cervical border to the 2/3 apical third. The samples were assigned randomly into three groups of 9 each, namely Group 1 with no application of any material in the fracture line (control group), Group 2 with MTA, and Group 3 with self-adhesive resin cement. All the teeth in all the groups were then inserted back into the socket. Each group was further divided into three subgroups based on the observation time, namely days 7, 14, and 21. Histological observations of the epithelial thickness were carried out under the light microscope (400x magnification). Data were analyzed using a two-way ANOVA and LSD post hoc test with a confidence level of 95%. The two-way ANOVA analysis showed that the materials used for sealing the fracture lines had a significant effect on the epithelial thickness (p<0.05), while the observation time did not affect the epithelial thickness (p>0.05). No interaction occurred between the material used and observation times (p>0.05). It can be concluded that MTA generated a greater epithelial thickness of periradicular tissue compared than self-adhesive resin cement in sealing vertical root fractures of intentional replantation.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81865439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.40196
Khong Mei Xuan, Anne Handrini Dewi, I. A. Wahyudi
Potato peel is often regarded as waste although it contains phenolic compounds, glycoalkaloids, and flavonoid. This study aimed to evaluate the effect of different concentrations of potato peel extracts on the viability of Human Gingival Fibroblasts (HGF). Potato peel extracts were prepared by a maceration technique. The 96-well tissue culture micro titre plates were seeded with HGF at a density of 2×104 cells/100 μL and incubated for 24 hours. Next, 100 μL of potato peel extracts at a concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL and a medium (control) were dispensed into the well of the cell culture. Each concentration was evaluated for its viability with 3 replicate samples. The results of the MTT test were analyzed statistically using one-way ANOVA and LSD test. The mean and standard deviation of the viable HGF after incubated with the potato peel extract at the concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL were 98.67% ± 3.56, 88.34% ± 0.79, 55.42% ± 3.96, 28.33% ± 0.60, and 26.26% ± 0.53, respectively. The percentage of non-viable HGF increased with an increase in the concentration of the potato peel extract. The ANOVA test result showed a significant influence of various concentrations of the potato peel extract on the viability of HGF (p<0.05). The result of the LSD-test showed a significant difference among all the treatment groups (p<0.05). A higher concentration of potato peel extracts increased the viability of HGF cell line and the concentrations of 62.5 μg/mL and 125 μg/mL were considered non-cytotoxic.
{"title":"In-vitro cytotoxicity activity of potato (Solanum tuberosum. L) peel extracts against human gingival fibroblasts","authors":"Khong Mei Xuan, Anne Handrini Dewi, I. A. Wahyudi","doi":"10.22146/majkedgiind.40196","DOIUrl":"https://doi.org/10.22146/majkedgiind.40196","url":null,"abstract":"Potato peel is often regarded as waste although it contains phenolic compounds, glycoalkaloids, and flavonoid. This study aimed to evaluate the effect of different concentrations of potato peel extracts on the viability of Human Gingival Fibroblasts (HGF). Potato peel extracts were prepared by a maceration technique. The 96-well tissue culture micro titre plates were seeded with HGF at a density of 2×104 cells/100 μL and incubated for 24 hours. Next, 100 μL of potato peel extracts at a concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL and a medium (control) were dispensed into the well of the cell culture. Each concentration was evaluated for its viability with 3 replicate samples. The results of the MTT test were analyzed statistically using one-way ANOVA and LSD test. The mean and standard deviation of the viable HGF after incubated with the potato peel extract at the concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL were 98.67% ± 3.56, 88.34% ± 0.79, 55.42% ± 3.96, 28.33% ± 0.60, and 26.26% ± 0.53, respectively. The percentage of non-viable HGF increased with an increase in the concentration of the potato peel extract. The ANOVA test result showed a significant influence of various concentrations of the potato peel extract on the viability of HGF (p<0.05). The result of the LSD-test showed a significant difference among all the treatment groups (p<0.05). A higher concentration of potato peel extracts increased the viability of HGF cell line and the concentrations of 62.5 μg/mL and 125 μg/mL were considered non-cytotoxic.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89093002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.60833
Nenny Wuri Prabawati, L. Hanindriyo, Sri Widiati
The selection of appropriate dental health education methods will be beneficial in promoting dental health. This study aimed to determine the difference in the effect of role-playing method and storytelling method on knowledge and attitudes towards oral hygiene among elementary school students. The research subjects were 112 students in grade 5. The subjects were divided into 2 different treatment groups, namely 56 students in grade 5 at SD Negeri Tegalrejo I with the storytelling method and 56 students in grade 5 at SD Negeri Tegalrejo II using the role-playing method. The measuring instrument in this research was a questionnaire. The data analysis used the Mann-Whitney test and Wilcoxon Signed Ranks test because the data were not normally distributed. The results of the analysis showed that there was a significant increase over time in knowledge and attitudes carried out in 3 assessments. The mean rank for delta values between the pre-test and posttest 2 for the knowledge variable using the role-playing method was 51.29 while that using the storytelling method was 61.71. Meanwhile, the mean rank for delta values for the attitude variable using the role-playing method was 49.93, while that using the storytelling method was 63.07. The results of the delta analysis from pre-test to post-test 1 and pre-test to post-test 2 showed that the storytelling group experiences a higher increase in knowledge and attitudes than the role-playing group (p<0.05). Provision of education using a storytelling method shows better improvement in students’ knowledge and attitudes towards oral hygiene than using a role-playing method.
选择合适的牙齿健康教育方法，有利于促进牙齿健康。本研究旨在探讨角色扮演法与讲故事法对小学生口腔卫生知识与态度的影响差异。研究对象为112名五年级学生。被试被分为2个不同的治疗组，56名小学五年级学生采用讲故事法，56名小学五年级学生采用角色扮演法。本研究的测量工具为问卷调查。数据分析采用Mann-Whitney检验和Wilcoxon Signed Ranks检验，因为数据不是正态分布。分析结果表明，随着时间的推移，在3个评估中进行的知识和态度显著增加。使用角色扮演方法的知识变量的前测与后测2的δ值平均秩为51.29，而使用讲故事方法的知识变量的δ值平均秩为61.71。同时，使用角色扮演方法的态度变量的δ值平均排名为49.93，而使用讲故事方法的态度变量的δ值平均排名为63.07。从前测到后测1和前测到后测2的增量分析结果显示，讲故事组的知识和态度的增长高于角色扮演组(p<0.05)。用讲故事的方式进行教育比用角色扮演的方式更能提高学生对口腔卫生的知识和态度。
{"title":"Educational approaches to improving knowledge and attitude towards dental hygiene among elementary school children","authors":"Nenny Wuri Prabawati, L. Hanindriyo, Sri Widiati","doi":"10.22146/majkedgiind.60833","DOIUrl":"https://doi.org/10.22146/majkedgiind.60833","url":null,"abstract":"The selection of appropriate dental health education methods will be beneficial in promoting dental health. This study aimed to determine the difference in the effect of role-playing method and storytelling method on knowledge and attitudes towards oral hygiene among elementary school students. The research subjects were 112 students in grade 5. The subjects were divided into 2 different treatment groups, namely 56 students in grade 5 at SD Negeri Tegalrejo I with the storytelling method and 56 students in grade 5 at SD Negeri Tegalrejo II using the role-playing method. The measuring instrument in this research was a questionnaire. The data analysis used the Mann-Whitney test and Wilcoxon Signed Ranks test because the data were not normally distributed. The results of the analysis showed that there was a significant increase over time in knowledge and attitudes carried out in 3 assessments. The mean rank for delta values between the pre-test and posttest 2 for the knowledge variable using the role-playing method was 51.29 while that using the storytelling method was 61.71. Meanwhile, the mean rank for delta values for the attitude variable using the role-playing method was 49.93, while that using the storytelling method was 63.07. The results of the delta analysis from pre-test to post-test 1 and pre-test to post-test 2 showed that the storytelling group experiences a higher increase in knowledge and attitudes than the role-playing group (p<0.05). Provision of education using a storytelling method shows better improvement in students’ knowledge and attitudes towards oral hygiene than using a role-playing method.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86324024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.65512
Ardy Setiawan, Juanita A. Gunawan, Selviana Wulansari, Didi Nugroho
Glass ionomer cement as one of the restoration materials requires high compressive strength so it can last during functional activity. The latest glass ionomer cement comes with glass hybrid technology and a nanofilled resin-based protective coating which is said to increase the compressive strength of glass ionomer cement. The aim of this study was to analyze the effect of nanofilled resin-based coating and the types of glass ionomer cement materials on their compressive strength. Two types of commercial glass ionomer cement material were used; conventional (Fuji IX GP Extra), and hybrid (EQUIA Forte Fill) glass ionomer cement. Forty cylindrical (4 x 6 mm) samples were prepared in each group. The main group was divided into 4 subgroups (n=10) based on the protective coating used (EQUIA Forte Coat, Varnish, Control, Water + EQUIA Forte Coat). Eight subgroups were immersed in 37 °C distilled water for 7 days, then a compressive strength test was performed using a universal testing machine. The data analysis showed no significant difference in the compressive strength between the two types of glass ionomer cement materials (p>0.05). The use of a protective coating was associated with a significant decrease in the compressive strength (p<0.05). The use of glass ionomer cement without the application of a protective coating was considered to be quite good because the compressive strength value of the restoration still met the standards of the American Dental Association.
玻璃离子水门合剂作为一种修复材料，要求具有较高的抗压强度，以保证其在功能活动中持续存在。最新的玻璃离子水泥采用了玻璃混合技术和纳米填充树脂基保护涂层，据说可以提高玻璃离子水泥的抗压强度。本研究的目的是分析纳米填充树脂基涂层和玻璃离子水泥材料的类型对其抗压强度的影响。采用了两种商用玻璃离子水泥材料;常规(Fuji IX GP Extra)和混合(EQUIA Forte Fill)玻璃离子水泥。每组制备40个圆柱形(4 × 6 mm)样品。根据使用的保护涂层(EQUIA Forte Coat, Varnish, Control, Water + EQUIA Forte Coat)将主组分为4个亚组(n=10)。8个亚组在37℃蒸馏水中浸泡7天，然后使用万能试验机进行抗压强度试验。数据分析显示，两种玻璃离子水门汀材料抗压强度差异无统计学意义(p < 0.05)。防护涂层的使用与抗压强度的显著降低相关(p<0.05)。使用玻璃离聚体水泥而不使用保护涂层被认为是相当好的，因为修复的抗压强度值仍然符合美国牙科协会的标准。
{"title":"Effect of Nanofilled Resin-Based Coating on the Compressive Strength of Glass Ionomer Cement – in vitro","authors":"Ardy Setiawan, Juanita A. Gunawan, Selviana Wulansari, Didi Nugroho","doi":"10.22146/majkedgiind.65512","DOIUrl":"https://doi.org/10.22146/majkedgiind.65512","url":null,"abstract":"Glass ionomer cement as one of the restoration materials requires high compressive strength so it can last during functional activity. The latest glass ionomer cement comes with glass hybrid technology and a nanofilled resin-based protective coating which is said to increase the compressive strength of glass ionomer cement. The aim of this study was to analyze the effect of nanofilled resin-based coating and the types of glass ionomer cement materials on their compressive strength. Two types of commercial glass ionomer cement material were used; conventional (Fuji IX GP Extra), and hybrid (EQUIA Forte Fill) glass ionomer cement. Forty cylindrical (4 x 6 mm) samples were prepared in each group. The main group was divided into 4 subgroups (n=10) based on the protective coating used (EQUIA Forte Coat, Varnish, Control, Water + EQUIA Forte Coat). Eight subgroups were immersed in 37 °C distilled water for 7 days, then a compressive strength test was performed using a universal testing machine. The data analysis showed no significant difference in the compressive strength between the two types of glass ionomer cement materials (p>0.05). The use of a protective coating was associated with a significant decrease in the compressive strength (p<0.05). The use of glass ionomer cement without the application of a protective coating was considered to be quite good because the compressive strength value of the restoration still met the standards of the American Dental Association.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80263324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.53419
Tissa Rahadianti, D. Herawati, K. Murdiastuti
Open flap debridement (OFD) is an invasive therapy for chronic periodontitis with pocket 5 mm or more. However, it is difficult to achieve regeneration and new attachment with this therapy. Periodontitis starts to add growth factors and local drugs delivery as host modulation therapy. Advanced-PRF (A-PRF) contains more growth factor than PRF which plays a role in promoting fibroblast proliferation, reepithelization, extracellular matrix production, and endothelial cell migration. 1.2% rosuvastatin gel (RSV) is a local delivery drug with a pleiotropic effect that can modify host response to promoting BMSCs, BMP-2, OPG, ALP, RANKL, and osteoblasts. This study aimed to examine the effect of the application of A-PRF+RSV in OFD therapy of which the parameters were probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The study samples consisted of 24 periodontal pockets which were divided into 2 groups of 12 pockets each, namely A-PRF+RSV for group 1 and PRF+RSV for group 2. Clinical evaluations were carried out on baseline, day-30, and day-90 for PD and RAL, and on baseline and day -90 for alveolar bone height. Data of PD and RAL reduction were analyzed with non-parametric test Mann-Withney, while data of reduction of alveolar bone height were analyzed with parametric Independent-T test. Group 1 obtained a statistically more significant result in reducing PD, RAL, and alveolar bone height compared to group 2 (p<0.05) To conclude, the application of A-PRF and 1.2% rosuvastatin gel in OFD procedure promotes a higher PD and RAL reduction and alveolar bone height increase than the application of PRF coupled with 1.2% rosuvastatin gel.
开放式皮瓣清创(OFD)是一种有创治疗慢性牙周炎口袋5毫米或以上。然而，这种疗法很难实现再生和新的附着。牙周炎开始添加生长因子和局部药物递送作为宿主调节治疗。Advanced-PRF (a -PRF)含有比PRF更多的生长因子，在促进成纤维细胞增殖、再上皮、细胞外基质生成和内皮细胞迁移等方面发挥作用。1.2%瑞舒伐他汀凝胶(RSV)是一种局部递送药物，具有多效性，可以改变宿主对促进骨髓间充质干细胞、BMP-2、OPG、ALP、RANKL和成骨细胞的反应。本研究旨在探讨A-PRF+RSV在OFD治疗中的应用效果，其参数为探探深度(PD)、相对附着损失(RAL)和牙槽骨高度。研究样本为24个牙周袋，分为2组，每组12个袋，1组为A-PRF+RSV, 2组为PRF+RSV。在基线、第30天和第90天对PD和RAL进行临床评估，在基线和第90天对牙槽骨高度进行临床评估。PD、RAL复位数据采用Mann-Withney非参数检验，牙槽骨高度复位数据采用参数独立t检验。组1在降低PD、RAL和牙槽骨高度方面的效果比组2有统计学意义(p<0.05)。综上所述，在OFD手术中，a -PRF联合1.2%瑞舒伐他汀凝胶比PRF联合1.2%瑞舒伐他汀凝胶对PD、RAL的降低和牙槽骨高度的增加有更高的促进作用。
{"title":"Effect of advanced-platelet rich-fibrin combined with rosuvastatin application after open flap debridement of infrabony pocket","authors":"Tissa Rahadianti, D. Herawati, K. Murdiastuti","doi":"10.22146/majkedgiind.53419","DOIUrl":"https://doi.org/10.22146/majkedgiind.53419","url":null,"abstract":"Open flap debridement (OFD) is an invasive therapy for chronic periodontitis with pocket 5 mm or more. However, it is difficult to achieve regeneration and new attachment with this therapy. Periodontitis starts to add growth factors and local drugs delivery as host modulation therapy. Advanced-PRF (A-PRF) contains more growth factor than PRF which plays a role in promoting fibroblast proliferation, reepithelization, extracellular matrix production, and endothelial cell migration. 1.2% rosuvastatin gel (RSV) is a local delivery drug with a pleiotropic effect that can modify host response to promoting BMSCs, BMP-2, OPG, ALP, RANKL, and osteoblasts. This study aimed to examine the effect of the application of A-PRF+RSV in OFD therapy of which the parameters were probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The study samples consisted of 24 periodontal pockets which were divided into 2 groups of 12 pockets each, namely A-PRF+RSV for group 1 and PRF+RSV for group 2. Clinical evaluations were carried out on baseline, day-30, and day-90 for PD and RAL, and on baseline and day -90 for alveolar bone height. Data of PD and RAL reduction were analyzed with non-parametric test Mann-Withney, while data of reduction of alveolar bone height were analyzed with parametric Independent-T test. Group 1 obtained a statistically more significant result in reducing PD, RAL, and alveolar bone height compared to group 2 (p<0.05) To conclude, the application of A-PRF and 1.2% rosuvastatin gel in OFD procedure promotes a higher PD and RAL reduction and alveolar bone height increase than the application of PRF coupled with 1.2% rosuvastatin gel.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"2015 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78715654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.22146/majkedgiind.62212
Dedy Agoes Mahendra, Y. Nindita, Gustantyo Wahyu Wibowo, Gloria Fortuna
Obturation with a sealer material that provides an adequate apical sealing ability is required to prevent endodontic treatment failure due to microleakage. However, there are no sealers that meet all the physical and chemical properties to be able to hermetically seal the root canal system to date. Various sealer materials have been developed in recent years including the use of bioceramic materials which are claimed to have excellent biocompatibility to tissues. This study aimed to compare the apical sealing ability of bioceramic-based and zinc oxide eugenol (ZOE)-based sealer in root canal treatment. A total of 27 extracted mandibular premolars were decoronated to the standard root length of 14 mm. The root canals were prepared with a crown-down technique using manual instrument to file F3 (30/.09). The samples were then divided into three groups: obturation with bioceramic-based sealer (n=9); ZOE-based sealer (n=9); and control group (n=9). Microleakage was measured using a dye penetration method with 1% methylene blue and observed under stereomicroscope at x20 magnification. The mean of the maximum penetration length from the lowest to the highest was found in the bioceramic-based sealer group (0.825 mm), the ZOE-based sealer group (3.850 mm), and the control group (4.444 mm). One-way ANOVA test showed a significant difference in the maximum penetration length between the three groups (p<0.05). The post hoc LSD test showed a significant difference in the maximum penetration length between the bioceramic-based and ZOE-based sealer groups (p<0.001). Obturation with bioceramic-based sealer provides a better apical sealing ability than that with ZOE-based sealer.
{"title":"Comparison of apical sealing ability between bioceramic and zinc oxide eugenol-based sealer during root canal treatment, in vitro","authors":"Dedy Agoes Mahendra, Y. Nindita, Gustantyo Wahyu Wibowo, Gloria Fortuna","doi":"10.22146/majkedgiind.62212","DOIUrl":"https://doi.org/10.22146/majkedgiind.62212","url":null,"abstract":"Obturation with a sealer material that provides an adequate apical sealing ability is required to prevent endodontic treatment failure due to microleakage. However, there are no sealers that meet all the physical and chemical properties to be able to hermetically seal the root canal system to date. Various sealer materials have been developed in recent years including the use of bioceramic materials which are claimed to have excellent biocompatibility to tissues. This study aimed to compare the apical sealing ability of bioceramic-based and zinc oxide eugenol (ZOE)-based sealer in root canal treatment. A total of 27 extracted mandibular premolars were decoronated to the standard root length of 14 mm. The root canals were prepared with a crown-down technique using manual instrument to file F3 (30/.09). The samples were then divided into three groups: obturation with bioceramic-based sealer (n=9); ZOE-based sealer (n=9); and control group (n=9). Microleakage was measured using a dye penetration method with 1% methylene blue and observed under stereomicroscope at x20 magnification. The mean of the maximum penetration length from the lowest to the highest was found in the bioceramic-based sealer group (0.825 mm), the ZOE-based sealer group (3.850 mm), and the control group (4.444 mm). One-way ANOVA test showed a significant difference in the maximum penetration length between the three groups (p<0.05). The post hoc LSD test showed a significant difference in the maximum penetration length between the bioceramic-based and ZOE-based sealer groups (p<0.001). Obturation with bioceramic-based sealer provides a better apical sealing ability than that with ZOE-based sealer.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87105593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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