Pub Date : 2021-06-22DOI: 10.15578/jpbkp.v16i1.682
Ellya Sinurat, Dina Fransiska, S. Sihono, Rinta Kusumawati
Rumput laut Ulva sp. memiliki kandungan serat pangan tinggi yang diketahui memiliki aktivitas hipoglikemik. Penelitian ini telah melakukan penambahan Ulva sp. pada biskuit sebagai makanan sehat yang kaya serat pangan. Tujuan penelitian untuk mengetahui pengaruh pemberian biskuit Ulva terhadap penurunan kadar glukosa darah pada tikus jantan yang diinduksi sukrosa jenuh. Selain itu, diamati profil hematologi dan biokimia klinis darah sebelum dan setelah pemberian biskuit Ulva. Uji antidiabetes dilakukan menggunakan uji toleransi glukosa oral terhadap tikus jantan yang diinduksi sukrosa jenuh. Biskuit Ulva yang diberikan 1 g/kg berat badan (BB) tikus dengan perlakuan kontrol negatif (pakan tanpa biskuit), biskuit tanpa Ulva sp., dan biskuit Ulva setara dengan Ulva sp. 1, 5, dan 10 mg/kg BB. Perlakuan dosis diberikan pada 5 ekor tikus percobaan sekali sehari selama 14 hari. Pengamatan terhadap intoleransi glukosa dilakukan melalui pengukuran glukosa darah setelah pemberian sukrosa jenuh ke semua perlakuan pada hari ke-14, dan diukur pada menit ke-0, 30, 60, dan 120. Penimbangan tikus dilakukan pada hari ke-0, 7, dan 14, sedangkan analisis hematologi dan biokimia klinis darah dilakukan pada hari ke-0 dan ke-14. Pemberian biskuit Ulva berpengaruh signifikan terhadap kadar glukosa darah, serta menurunkan hematokrit dan hemoglobin darah tikus. Biskuit dengan dosis Ulva setara 1 mg/kg BB mampu menurunkan kadar glukosa darah tikus secara efektif pada menit ke-60. Tidak ada perbedaan kenaikan berat badan tikus jantan antara kelompok kontrol negatif dan biskuit Ulva pada hari ke-0, 7, dan 14. Pemberian biskuit Ulva sampai dengan 10 mg/kg BB tidak mempengaruhi SGOT, SGPT, ureum, dan kreatinin tikus. ABSTRACTSeaweed Ulva sp. contains high dietary fiber which is known to have hypoglycemic activity. In this study, the addition of Ulva sp. in biscuit products as a healthy food rich in dietary fiber. The objective of this study was to determine the effect of Ulva added biscuits on blood glucose levels reduction in male rats induced by saturated sucrose. In addition, clinical hematology and blood biochemical profiles before and after the administration of Ulva biscuits were also observed. Antidiabetic method used the oral glucose intolerance test method on male rats induced by saturated sucrose. Ulva biscuits were given at 1 g/kg body weight of rats for each treatment. This test used five treatments, namely negative control (rats feeding without biscuits), rat feeding without Ulva added biscuits; and rat feeding with Ulva biscuits equivalent to 1, 5, and 10 mg Ulva sp. /kg BW. Each dose treatment was given to five experimental rats once a day for 14 days. Observations on glucose intolerance included measurement of blood glucose levels by giving saturated sucrose to all treatments and measured at 0, 30, 60, and 120 minutes after administration of saturated sucrose. The weighing was carried out on day 0, 7, and 14, while clinical hematological and blood biochemical a
{"title":"Efek Pemberian Biskuit Ulva terhadap Penurunan Kadar Glukosa Darah Tikus yang Diinduksi Sukrosa","authors":"Ellya Sinurat, Dina Fransiska, S. Sihono, Rinta Kusumawati","doi":"10.15578/jpbkp.v16i1.682","DOIUrl":"https://doi.org/10.15578/jpbkp.v16i1.682","url":null,"abstract":"Rumput laut Ulva sp. memiliki kandungan serat pangan tinggi yang diketahui memiliki aktivitas hipoglikemik. Penelitian ini telah melakukan penambahan Ulva sp. pada biskuit sebagai makanan sehat yang kaya serat pangan. Tujuan penelitian untuk mengetahui pengaruh pemberian biskuit Ulva terhadap penurunan kadar glukosa darah pada tikus jantan yang diinduksi sukrosa jenuh. Selain itu, diamati profil hematologi dan biokimia klinis darah sebelum dan setelah pemberian biskuit Ulva. Uji antidiabetes dilakukan menggunakan uji toleransi glukosa oral terhadap tikus jantan yang diinduksi sukrosa jenuh. Biskuit Ulva yang diberikan 1 g/kg berat badan (BB) tikus dengan perlakuan kontrol negatif (pakan tanpa biskuit), biskuit tanpa Ulva sp., dan biskuit Ulva setara dengan Ulva sp. 1, 5, dan 10 mg/kg BB. Perlakuan dosis diberikan pada 5 ekor tikus percobaan sekali sehari selama 14 hari. Pengamatan terhadap intoleransi glukosa dilakukan melalui pengukuran glukosa darah setelah pemberian sukrosa jenuh ke semua perlakuan pada hari ke-14, dan diukur pada menit ke-0, 30, 60, dan 120. Penimbangan tikus dilakukan pada hari ke-0, 7, dan 14, sedangkan analisis hematologi dan biokimia klinis darah dilakukan pada hari ke-0 dan ke-14. Pemberian biskuit Ulva berpengaruh signifikan terhadap kadar glukosa darah, serta menurunkan hematokrit dan hemoglobin darah tikus. Biskuit dengan dosis Ulva setara 1 mg/kg BB mampu menurunkan kadar glukosa darah tikus secara efektif pada menit ke-60. Tidak ada perbedaan kenaikan berat badan tikus jantan antara kelompok kontrol negatif dan biskuit Ulva pada hari ke-0, 7, dan 14. Pemberian biskuit Ulva sampai dengan 10 mg/kg BB tidak mempengaruhi SGOT, SGPT, ureum, dan kreatinin tikus. ABSTRACTSeaweed Ulva sp. contains high dietary fiber which is known to have hypoglycemic activity. In this study, the addition of Ulva sp. in biscuit products as a healthy food rich in dietary fiber. The objective of this study was to determine the effect of Ulva added biscuits on blood glucose levels reduction in male rats induced by saturated sucrose. In addition, clinical hematology and blood biochemical profiles before and after the administration of Ulva biscuits were also observed. Antidiabetic method used the oral glucose intolerance test method on male rats induced by saturated sucrose. Ulva biscuits were given at 1 g/kg body weight of rats for each treatment. This test used five treatments, namely negative control (rats feeding without biscuits), rat feeding without Ulva added biscuits; and rat feeding with Ulva biscuits equivalent to 1, 5, and 10 mg Ulva sp. /kg BW. Each dose treatment was given to five experimental rats once a day for 14 days. Observations on glucose intolerance included measurement of blood glucose levels by giving saturated sucrose to all treatments and measured at 0, 30, 60, and 120 minutes after administration of saturated sucrose. The weighing was carried out on day 0, 7, and 14, while clinical hematological and blood biochemical a","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48942684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-04DOI: 10.15578/JPBKP.V16I1.674
Wiwin Kusuma Perdana Sari, S. Suharyanto
Makroalga merupakan biota laut yang mengandung berbagai jenis senyawa potensial, termasuk pigmen. Pigmen alami makroalga telah terbukti menunjukkan berbagai aktivitas biologis yang bermanfaat, termasuk sebagai antioksidan. Meskipun informasi mengenai kandungan pigmen maupun potensi antioksidan makroalga dari Pantai Gunungkidul telah banyak diteliti, namun penelitian yang menelaah korelasi antara kandungan pigmen dan aktivitas antioksidan makroalga dari wilayah ini masih terbatas. Berdasarkan hal tersebut, penelitian ini bertujuan untuk mengkaji korelasi kandungan pigmen serta potensi antioksidan beberapa jenis makroalga yang berasal dari Pantai Gunungkidul, Yogyakarta. Enam spesies mewakili kelompok makroalga hijau, merah, dan coklat digunakan pada penelitian ini, yaitu Codium tomentosum, Ulva lactuca, Gelidium corneum, Palisada perforata, Turbinaria ornata, dan Sargassum aquifolium. Hasil pengukuran kandungan pigmen menunjukkan bahwa klorofil a tertinggi terdapat pada jenis U. lactuca, klorofil b tertinggi pada C. tomentosum, dan total karotenoid tertinggi pada T. ornata. Dari keenam spesies yang diteliti, T. ornata menunjukkan potensi antioksidan terbaik dalam menangkap radikal bebas DPPH dengan persentase penghambatan radikal DPPH 79,11±0,41% pada konsentrasi 500 ppm. Analisis korelasi Pearson menunjukkan bahwa potensi antioksidan makroalga berkorelasi positif dengan kandungan total karotenoid (R2=0,806; p<0,05), dan tidak berkorelasi dengan kandungan klorofilnya (R2=0,044; p>0,05). Hasil studi ini menunjukkan bahwa karotenoid dari berbagai jenis makroalga merupakan kelompok pigmen yang potensial untuk dikembangkan sebagai nutrasetikal antioksidan. ABSTRACTMacroalgae are marine biota that contains various types of potential compounds, including pigments. Natural pigments of macroalgae have shown to produce various beneficial biological activities, including antioxidant. Although information regarding the pigment content and antioxidant property of macroalgae from Gunungkidul Coast has been widely studied, research on the correlation between pigment content and its antioxidant activity of macroalgae from this region is still limited. This study aimed to examine the correlation between pigment content of several species of macroalgae from Gunungkidul, Yogyakarta, and its antioxidant potential. Six species representing the green, red, and brown macroalgae were used in this study i.e. Codium tomentosum, Ulva lactuca, Gelidium corneum, Palisada perforata, Turbinaria ornata, and Sargassum aquifolium Result of pigment content concentration showed that the highest concentration of chlorophyll a was in U. lactuca, while chlorophyll b was in C. tomentosum, and the highest total carotenoid content was in T. ornata. Of the six species assessed, the brown macroalgae T. ornata showed the best antioxidant potential in DPPH radical scavenging with a percentage of DPPH inhibition 79.11±0.41% at 500 ppm. The Pearson correlation analysis showed that the antioxid
{"title":"Kandungan Pigmen dan Potensi Antioksidan Beberapa Jenis Makroalga dari Pantai Gunungkidul, Yogyakarta","authors":"Wiwin Kusuma Perdana Sari, S. Suharyanto","doi":"10.15578/JPBKP.V16I1.674","DOIUrl":"https://doi.org/10.15578/JPBKP.V16I1.674","url":null,"abstract":"Makroalga merupakan biota laut yang mengandung berbagai jenis senyawa potensial, termasuk pigmen. Pigmen alami makroalga telah terbukti menunjukkan berbagai aktivitas biologis yang bermanfaat, termasuk sebagai antioksidan. Meskipun informasi mengenai kandungan pigmen maupun potensi antioksidan makroalga dari Pantai Gunungkidul telah banyak diteliti, namun penelitian yang menelaah korelasi antara kandungan pigmen dan aktivitas antioksidan makroalga dari wilayah ini masih terbatas. Berdasarkan hal tersebut, penelitian ini bertujuan untuk mengkaji korelasi kandungan pigmen serta potensi antioksidan beberapa jenis makroalga yang berasal dari Pantai Gunungkidul, Yogyakarta. Enam spesies mewakili kelompok makroalga hijau, merah, dan coklat digunakan pada penelitian ini, yaitu Codium tomentosum, Ulva lactuca, Gelidium corneum, Palisada perforata, Turbinaria ornata, dan Sargassum aquifolium. Hasil pengukuran kandungan pigmen menunjukkan bahwa klorofil a tertinggi terdapat pada jenis U. lactuca, klorofil b tertinggi pada C. tomentosum, dan total karotenoid tertinggi pada T. ornata. Dari keenam spesies yang diteliti, T. ornata menunjukkan potensi antioksidan terbaik dalam menangkap radikal bebas DPPH dengan persentase penghambatan radikal DPPH 79,11±0,41% pada konsentrasi 500 ppm. Analisis korelasi Pearson menunjukkan bahwa potensi antioksidan makroalga berkorelasi positif dengan kandungan total karotenoid (R2=0,806; p<0,05), dan tidak berkorelasi dengan kandungan klorofilnya (R2=0,044; p>0,05). Hasil studi ini menunjukkan bahwa karotenoid dari berbagai jenis makroalga merupakan kelompok pigmen yang potensial untuk dikembangkan sebagai nutrasetikal antioksidan. ABSTRACTMacroalgae are marine biota that contains various types of potential compounds, including pigments. Natural pigments of macroalgae have shown to produce various beneficial biological activities, including antioxidant. Although information regarding the pigment content and antioxidant property of macroalgae from Gunungkidul Coast has been widely studied, research on the correlation between pigment content and its antioxidant activity of macroalgae from this region is still limited. This study aimed to examine the correlation between pigment content of several species of macroalgae from Gunungkidul, Yogyakarta, and its antioxidant potential. Six species representing the green, red, and brown macroalgae were used in this study i.e. Codium tomentosum, Ulva lactuca, Gelidium corneum, Palisada perforata, Turbinaria ornata, and Sargassum aquifolium Result of pigment content concentration showed that the highest concentration of chlorophyll a was in U. lactuca, while chlorophyll b was in C. tomentosum, and the highest total carotenoid content was in T. ornata. Of the six species assessed, the brown macroalgae T. ornata showed the best antioxidant potential in DPPH radical scavenging with a percentage of DPPH inhibition 79.11±0.41% at 500 ppm. The Pearson correlation analysis showed that the antioxid","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48859466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-04DOI: 10.15578/JPBKP.V16I1.681
Jamal Basmal, N. Nurhayati
Caulerpa racemosa mengandung serat, vitamin, antioksidan, polisakarida sulfat, sulfat bebas, dan mineral sehingga dapat dimanfaatkan sebagai pangan fungsional. Namun, cairan C. racemosa mudah mengalami kemunduran mutu selama proses penyimpanan dan transportasi, sehingga perlu dipertahankan mutunya dalam bentuk bulir. Perlakuan yang diberikan dalam proses penyalutan adalah variasi konsentrasi Na-alginat 0,4%; 0,6% dan 0,8% dan variasi larutan Ca-laktat 0,4%; 0,6% dan 0,8% yang diulang sebanyak tiga kali. Tujuan penelitian ini untuk menentukan kombinasi konsentrasi Na-alginat dan Ca-laktat terbaik dalam mempertahankan mutu bulir cairan C. racemosa. Hasil penelitian menunjukkan bahwa cairan C. racemosa mengandung mineral, serat pangan, dan asam amino baik esensial maupun non esensial dengan kadar air cairan C. racemosa sebesar 97,77%±0,02% dan setelah ditambah air dengan rasio 1:4, kadar airnya menjadi 99,6%±0,61%. Perlakuan konsentrasi Ca-laktat berpengaruh terhadap kadar air dan kadar abu bulir yang dihasilkan, sementara kadar serat kasar bulir dipengaruhi oleh perbedaan konsentrasi Na-alginat. Nilai terbaik berdasarkan kadar serat ditemukan pada perlakuan kombinasi Na-alginat 0,6% dan Ca-laktat 0,8% sebesar 4,05% dengan kadar air 97,22% dan kadar abu 0,65%.ABSTRACTCaulerpa racemosa contains fiber, vitamins, antioxidants, sulfated polysaccharides, free sulfates, and minerals so that it can be used as functional food. However, C. racemosa liquid deteriorates easily during storage and transportation, therefore it is necessary to maintain the quality of C. racemosa liquid in encapsulated form as grain. The treatments given in encapsulation process were variation of Na-alginat concentration i.e. 0.4%; 0.6% and 0.8%, and variation of Ca-lactate solution i.e. 0.4%; 0.6% and 0.8% which were repeated three times. The aim of the study was to determine the best combination of Na-alginate and Ca-lactate concentrations in maintaining the quality of encapsulated C. racemosa liquid grain. The results showed that C. racemosa liquid contained minerals, dietary fiber and amino acids, both essential and non-essential, with moisture content of 97.77±0.02%. After added with water with a ratio of 1:4, the moisture content of C. racemosa liquid was 99.6%±0.61%. The treatment of Ca-lactate concentration affected the moisture content and ash content of the liquid grain, while the crude fiber content was affected by Na-alginate concentration. The best value based on fiber content was found in the combination treatment of 0.6% Na-alginate and 0.8% Ca-lactate (A6K8) at 4.05%, with moisture content of 97.22% and an ash content of 0.65%.
{"title":"Kualitas Bulir Cairan Caulerpa racemosa yang Disalut dengan Na-Alginat dan Ca-Laktat","authors":"Jamal Basmal, N. Nurhayati","doi":"10.15578/JPBKP.V16I1.681","DOIUrl":"https://doi.org/10.15578/JPBKP.V16I1.681","url":null,"abstract":"Caulerpa racemosa mengandung serat, vitamin, antioksidan, polisakarida sulfat, sulfat bebas, dan mineral sehingga dapat dimanfaatkan sebagai pangan fungsional. Namun, cairan C. racemosa mudah mengalami kemunduran mutu selama proses penyimpanan dan transportasi, sehingga perlu dipertahankan mutunya dalam bentuk bulir. Perlakuan yang diberikan dalam proses penyalutan adalah variasi konsentrasi Na-alginat 0,4%; 0,6% dan 0,8% dan variasi larutan Ca-laktat 0,4%; 0,6% dan 0,8% yang diulang sebanyak tiga kali. Tujuan penelitian ini untuk menentukan kombinasi konsentrasi Na-alginat dan Ca-laktat terbaik dalam mempertahankan mutu bulir cairan C. racemosa. Hasil penelitian menunjukkan bahwa cairan C. racemosa mengandung mineral, serat pangan, dan asam amino baik esensial maupun non esensial dengan kadar air cairan C. racemosa sebesar 97,77%±0,02% dan setelah ditambah air dengan rasio 1:4, kadar airnya menjadi 99,6%±0,61%. Perlakuan konsentrasi Ca-laktat berpengaruh terhadap kadar air dan kadar abu bulir yang dihasilkan, sementara kadar serat kasar bulir dipengaruhi oleh perbedaan konsentrasi Na-alginat. Nilai terbaik berdasarkan kadar serat ditemukan pada perlakuan kombinasi Na-alginat 0,6% dan Ca-laktat 0,8% sebesar 4,05% dengan kadar air 97,22% dan kadar abu 0,65%.ABSTRACTCaulerpa racemosa contains fiber, vitamins, antioxidants, sulfated polysaccharides, free sulfates, and minerals so that it can be used as functional food. However, C. racemosa liquid deteriorates easily during storage and transportation, therefore it is necessary to maintain the quality of C. racemosa liquid in encapsulated form as grain. The treatments given in encapsulation process were variation of Na-alginat concentration i.e. 0.4%; 0.6% and 0.8%, and variation of Ca-lactate solution i.e. 0.4%; 0.6% and 0.8% which were repeated three times. The aim of the study was to determine the best combination of Na-alginate and Ca-lactate concentrations in maintaining the quality of encapsulated C. racemosa liquid grain. The results showed that C. racemosa liquid contained minerals, dietary fiber and amino acids, both essential and non-essential, with moisture content of 97.77±0.02%. After added with water with a ratio of 1:4, the moisture content of C. racemosa liquid was 99.6%±0.61%. The treatment of Ca-lactate concentration affected the moisture content and ash content of the liquid grain, while the crude fiber content was affected by Na-alginate concentration. The best value based on fiber content was found in the combination treatment of 0.6% Na-alginate and 0.8% Ca-lactate (A6K8) at 4.05%, with moisture content of 97.22% and an ash content of 0.65%.","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45232092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-04DOI: 10.15578/JPBKP.V16I1.699
D. S. Zilda, Gintung Patantis, Mada Triandala Sibero, Yusro Nuri Fawzya
Agarase adalah enzim yang mampu menghidrolisis agar menjadi oligoagar yang sudah banyak diaplikasikan dalam industri kesehatan dan kosmetik. Bakteri laut merupakan mikroba yang paling banyak dilaporkan sebagai sumber untuk isolasi bakteri penghasil agarase. Penelitian ini bertujuan untuk melakukan penapisan, isolasi, dan identifikasi bakteri penghasil agarase dari sedimen laut. Sampel sedimen diambil dari pantai Pulau Bara Caddi, Sulawesi Selatan. Penapisan dilakukan menggunakan media air laut yang ditambahkan tripton 0,5%, ekstrak ragi 0,1%, dan agar 2%. Identifikasi dilakukan dengan amplifikasi gen 16S rRNA. Sebanyak 45 isolat berhasil dimurnikan, 16 diantaranya merupakan bakteri penghasil agarase. Pola zona bening yang terbentuk terlihat berbeda-beda, hal ini diduga disebabkan oleh perbedaan jenis agarase yang dihasilkan oleh masing-masing isolat. Hasil penelitian menunjukkan bahwa terdapat 4 genera bakteri yang memiliki kemiripan yang tinggi dengan 16 isolat bakteri penghasil agarase yang terdapat pada sampel sedimen yaitu Vibrio, Alteromonas, Salinivibrio, dan Marinobacter. Vibrio merupakan genus yang paling dominan diikuti oleh Alteromonas dan hanya satu isolat yang menunjukkan kesamaan dengan Salinivibrio dan Marinobacter. ABSTRACTAgarase is an enzyme that hydrolyze agar into agaro oligosaccharide which have been applied in health and cosmetic industries. Marine bacteria are the most widely reported microbes as a source for isolation of agarase-producing bacteria. This work was aimed to screen, isolate, and identify the agarase-producing bacteria from marine sediment. The sediment samples were collected from the sea around Bara Caddi Island, South Sulawesi. The screening of agarase-producing bacteria was carried out using seawater media containing 0.5% tryptone, 0.1 % yeast extract with 2 % agar. The identification of the bacteria obtained was carried out by amplification of the 16S rRNA gene. A total of 45 isolates were successfully purified, 16 of which were agarase-producing bacteria. The clear zone formed on solid medium by some isolates showed different pattern which may be caused by the type of agarase produced by each isolate. The results showed that there were 4 genera of bacteria which similar to the 16 isolates agarase-producing bacteria found in sediment samples i.e. Vibrio, Alteromonas, Salinivibrio, and Marinobacter. Vibrio is the most dominant genus followed by Alteromonas and only one isolate showed similarity to Salinivibrio and Marinobacter.
{"title":"Penapisan dan Identifikasi Bakteri Penghasil Agarase dari Sampel Sedimen Laut Bara Caddi, Sulawesi Selatan","authors":"D. S. Zilda, Gintung Patantis, Mada Triandala Sibero, Yusro Nuri Fawzya","doi":"10.15578/JPBKP.V16I1.699","DOIUrl":"https://doi.org/10.15578/JPBKP.V16I1.699","url":null,"abstract":"Agarase adalah enzim yang mampu menghidrolisis agar menjadi oligoagar yang sudah banyak diaplikasikan dalam industri kesehatan dan kosmetik. Bakteri laut merupakan mikroba yang paling banyak dilaporkan sebagai sumber untuk isolasi bakteri penghasil agarase. Penelitian ini bertujuan untuk melakukan penapisan, isolasi, dan identifikasi bakteri penghasil agarase dari sedimen laut. Sampel sedimen diambil dari pantai Pulau Bara Caddi, Sulawesi Selatan. Penapisan dilakukan menggunakan media air laut yang ditambahkan tripton 0,5%, ekstrak ragi 0,1%, dan agar 2%. Identifikasi dilakukan dengan amplifikasi gen 16S rRNA. Sebanyak 45 isolat berhasil dimurnikan, 16 diantaranya merupakan bakteri penghasil agarase. Pola zona bening yang terbentuk terlihat berbeda-beda, hal ini diduga disebabkan oleh perbedaan jenis agarase yang dihasilkan oleh masing-masing isolat. Hasil penelitian menunjukkan bahwa terdapat 4 genera bakteri yang memiliki kemiripan yang tinggi dengan 16 isolat bakteri penghasil agarase yang terdapat pada sampel sedimen yaitu Vibrio, Alteromonas, Salinivibrio, dan Marinobacter. Vibrio merupakan genus yang paling dominan diikuti oleh Alteromonas dan hanya satu isolat yang menunjukkan kesamaan dengan Salinivibrio dan Marinobacter. ABSTRACTAgarase is an enzyme that hydrolyze agar into agaro oligosaccharide which have been applied in health and cosmetic industries. Marine bacteria are the most widely reported microbes as a source for isolation of agarase-producing bacteria. This work was aimed to screen, isolate, and identify the agarase-producing bacteria from marine sediment. The sediment samples were collected from the sea around Bara Caddi Island, South Sulawesi. The screening of agarase-producing bacteria was carried out using seawater media containing 0.5% tryptone, 0.1 % yeast extract with 2 % agar. The identification of the bacteria obtained was carried out by amplification of the 16S rRNA gene. A total of 45 isolates were successfully purified, 16 of which were agarase-producing bacteria. The clear zone formed on solid medium by some isolates showed different pattern which may be caused by the type of agarase produced by each isolate. The results showed that there were 4 genera of bacteria which similar to the 16 isolates agarase-producing bacteria found in sediment samples i.e. Vibrio, Alteromonas, Salinivibrio, and Marinobacter. Vibrio is the most dominant genus followed by Alteromonas and only one isolate showed similarity to Salinivibrio and Marinobacter.","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43988052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-04DOI: 10.15578/JPBKP.V16I1.696
Endar Marraskuranto, M. Nursid, Swestri Utami, Iriani Setyaningsih, Kustiariyah Tarman
Caulerpa racemosa merupakan rumput laut hijau yang mengandung senyawa aktif yang berpotensi sebagai antibakteri dan antioksidan. Perbedaan profil fitokimia dan bioaktivitas suatu ekstrak dipengaruhi oleh kepolaran pelarut ekstraksi. Penelitian ini bertujuan untuk mempelajari profil fitokimia, aktivitas antibakteri dan antioksidan ekstrak C. racemosa. Ekstrak diperoleh dari sampel segar C. racemosa yang dimaserasi menggunakan pelarut n-heksana, etil asetat, dan metanol. Uji antibakteri dilakukan dengan metode Resazurin Microtitter Assay (REMA) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Aktivitas antioksidan diuji menggunakan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH) dan Ferric Reducing Antioxidant Power (FRAP). Hasil penapisan fitokimia memperlihatkan bahwa ekstrak n-heksana mengandung alkaloid dan triterpenoid, sedangkan ekstrak etil asetat dan metanol mengandung alkaloid, flavonoid, tanin, fenol, dan steroid. Aktivitas antibakteri terbaik ditunjukkan oleh ekstrak etil asetat dengan nilai Minimum Inhibitory Concentration (MIC) terhadap E. coli sebesar 250 mg/mL. Ekstrak etil asetat C. racemosa juga memperlihatkan aktivitas antioksidan terbaik dengan nilai IC50 DPPH sebesar 110,7 mg/mL dan nilai FRAP sebesar 96,68 mmol Fe (II)/g. ABSTRACTCaulerpa racemosa is a green seaweed that contains active compounds that are potential for antibacterial and antioxidant. The phytochemical constituents and bioactivity of an extract depend on the extraction solvent polarity. This study aimed to determine the phytochemical profiles, antibacterial and antioxidant activity of C. racemosa extracts. Extracts were obtained from the fresh C. racemosa samples that were macerated with n-hexane, ethyl acetate, and methanol. Antibacterial assay was tested by Resazurin Microtiter Assay (REMA) method against Escherichia coli and Staphylococcus aureus. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) method. Phytochemical screening showed that n-hexane extract contained alkaloid and triterpenoid, while ethyl acetate and methanol extracts contained alkaloids, flavonoids, tannins, phenols, and steroids. The most active extract in the antibacterial assay was ethyl acetate extract with a Minimum Inhibitory Concentration (MIC) value of 250 mg/mL against E. coli. Ethyl acetate extract of C. racemosa also showed the best antioxidant activity where the IC50 of DPPH value was 110.7 mg/mL and the FRAP value was 96.68 mmol Fe(II)/g.
{"title":"Kandungan Fitokimia, Potensi Antibakteri dan Antioksidan Hasil Ekstraksi Caulerpa racemosa dengan Pelarut Berbeda","authors":"Endar Marraskuranto, M. Nursid, Swestri Utami, Iriani Setyaningsih, Kustiariyah Tarman","doi":"10.15578/JPBKP.V16I1.696","DOIUrl":"https://doi.org/10.15578/JPBKP.V16I1.696","url":null,"abstract":"Caulerpa racemosa merupakan rumput laut hijau yang mengandung senyawa aktif yang berpotensi sebagai antibakteri dan antioksidan. Perbedaan profil fitokimia dan bioaktivitas suatu ekstrak dipengaruhi oleh kepolaran pelarut ekstraksi. Penelitian ini bertujuan untuk mempelajari profil fitokimia, aktivitas antibakteri dan antioksidan ekstrak C. racemosa. Ekstrak diperoleh dari sampel segar C. racemosa yang dimaserasi menggunakan pelarut n-heksana, etil asetat, dan metanol. Uji antibakteri dilakukan dengan metode Resazurin Microtitter Assay (REMA) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Aktivitas antioksidan diuji menggunakan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH) dan Ferric Reducing Antioxidant Power (FRAP). Hasil penapisan fitokimia memperlihatkan bahwa ekstrak n-heksana mengandung alkaloid dan triterpenoid, sedangkan ekstrak etil asetat dan metanol mengandung alkaloid, flavonoid, tanin, fenol, dan steroid. Aktivitas antibakteri terbaik ditunjukkan oleh ekstrak etil asetat dengan nilai Minimum Inhibitory Concentration (MIC) terhadap E. coli sebesar 250 mg/mL. Ekstrak etil asetat C. racemosa juga memperlihatkan aktivitas antioksidan terbaik dengan nilai IC50 DPPH sebesar 110,7 mg/mL dan nilai FRAP sebesar 96,68 mmol Fe (II)/g. ABSTRACTCaulerpa racemosa is a green seaweed that contains active compounds that are potential for antibacterial and antioxidant. The phytochemical constituents and bioactivity of an extract depend on the extraction solvent polarity. This study aimed to determine the phytochemical profiles, antibacterial and antioxidant activity of C. racemosa extracts. Extracts were obtained from the fresh C. racemosa samples that were macerated with n-hexane, ethyl acetate, and methanol. Antibacterial assay was tested by Resazurin Microtiter Assay (REMA) method against Escherichia coli and Staphylococcus aureus. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) method. Phytochemical screening showed that n-hexane extract contained alkaloid and triterpenoid, while ethyl acetate and methanol extracts contained alkaloids, flavonoids, tannins, phenols, and steroids. The most active extract in the antibacterial assay was ethyl acetate extract with a Minimum Inhibitory Concentration (MIC) value of 250 mg/mL against E. coli. Ethyl acetate extract of C. racemosa also showed the best antioxidant activity where the IC50 of DPPH value was 110.7 mg/mL and the FRAP value was 96.68 mmol Fe(II)/g.","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48730188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-08DOI: 10.15578/jpbkp.v15i2.657
Ryana Tammi Putri, Linawati Hardjito, J. Santoso
Ulvan dalam hidrolisat Ulva lactuca merupakan senyawa bioaktif yang potensial di bidang biofarmakologi. Proses hidrolisis untuk memperoleh golongan senyawa ini dapat dilakukan secara kimiawi atau mikrobiologi. Proses mikrobiologi merupakan teknik yang potensial karena lebih mudah dan berbiaya rendah dibanding metode kimiawi. Namun, penelitian yang menggunakan teknik hidrolisis mikrobiologi masih sangat terbatas, terutama terhadap U. lactuca. Oleh karena itu, optimasi diperlukan agar mendapatkan teknik dengan hasil rendemen hidrolisat yang maksimal. Penelitian ini bertujuan untuk melakukan optimasi proses hidrolisis secara mikrobiologi dan menelaah aktivitas biologis ekstrak hidrolisatnya. Proses hidrolisis mikrobiologi dilakukan dengan teknik padat dan cair menggunakan kapang endofit dari tanaman Enhalus dan isolat bakteri laut dari rumput laut Eucheuma cottonii. Teknik mikrobiologis ini juga dibandingkan dengan teknik kimiawi (HCl). Optimasi dilakukan terhadap variabel durasi waktu (3, 6, 9, dan 12 hari) hidrolisis. Hasil penelitian menunjukkan bahwa rendemen metode hidrolisis cair menggunakan bantuan kapang endofit dari tanaman Enhalus (51,67%) dan isolat bakteri laut dari rumput laut E. cottonii (52,67%), lebih tinggi dan berbeda secara signifikan (p<0,05) dibandingkan teknik kimiawi (37,02%). Waktu hidrolisis terbaik yang ditemukan adalah selama tiga hari. Sementara itu, karakterisasi bioaktivitas menunjukkan bahwa hidrolisat U. lactuca memiliki potensi sebagai bahan aktif antikoagulan, tanpa adanya aktivitas sebagai antioksidan (IC50>200 ppm) maupun antibakteri (menghambat 36% Staphylococcus aureus dan Pseudomonas aeroginosa).ABSTRACTThe Ulvan in Ulva lactuca hydrolysate is a potential bioactive compound in the biopharmacology field. The hydrolysis process to obtain this class of compounds can be done in various ways, both chemically and microbiologically. The microbiological process is a potential technique because it is easier and cheaper than the chemical method. However, studies conducted with the microbiology hydrolysis technique are still very limited, especially for U. lactuca. Thus, optimation is needed to reveal an appropriate technique that produces the maximum yield. This study aimed to optimize the microbiological hydrolysis process and identify the biological activity of the hydrolyzed extract. The microbiological hydrolysis process was performed by solid and liquid techniques using microbial endophytic fungi from Enhalus plants and marine bacteria isolated from Eucheuma cottonii. The microbiology technique was compared with chemical techniques (HCl). Optimization was also carried out on the variable of hydrolysis duration (3, 6, 9, and 12 days). The results showed that yield of liquid hydrolysis method using the microbial endophytic fungi from Enhalus plants (51.67%) and marine bacteria isolated from E. cottonii (52.67%), were higher and significantly different (p<0.05) than that of chemical techniques (37.02%). Moreover, the b
{"title":"Optimasi Hidrolisis Mikrobiologi serta Bioaktivitas Antibakteri, Antioksidan, dan Antikoagulan Hidrolisat Ulva lactuca","authors":"Ryana Tammi Putri, Linawati Hardjito, J. Santoso","doi":"10.15578/jpbkp.v15i2.657","DOIUrl":"https://doi.org/10.15578/jpbkp.v15i2.657","url":null,"abstract":"Ulvan dalam hidrolisat Ulva lactuca merupakan senyawa bioaktif yang potensial di bidang biofarmakologi. Proses hidrolisis untuk memperoleh golongan senyawa ini dapat dilakukan secara kimiawi atau mikrobiologi. Proses mikrobiologi merupakan teknik yang potensial karena lebih mudah dan berbiaya rendah dibanding metode kimiawi. Namun, penelitian yang menggunakan teknik hidrolisis mikrobiologi masih sangat terbatas, terutama terhadap U. lactuca. Oleh karena itu, optimasi diperlukan agar mendapatkan teknik dengan hasil rendemen hidrolisat yang maksimal. Penelitian ini bertujuan untuk melakukan optimasi proses hidrolisis secara mikrobiologi dan menelaah aktivitas biologis ekstrak hidrolisatnya. Proses hidrolisis mikrobiologi dilakukan dengan teknik padat dan cair menggunakan kapang endofit dari tanaman Enhalus dan isolat bakteri laut dari rumput laut Eucheuma cottonii. Teknik mikrobiologis ini juga dibandingkan dengan teknik kimiawi (HCl). Optimasi dilakukan terhadap variabel durasi waktu (3, 6, 9, dan 12 hari) hidrolisis. Hasil penelitian menunjukkan bahwa rendemen metode hidrolisis cair menggunakan bantuan kapang endofit dari tanaman Enhalus (51,67%) dan isolat bakteri laut dari rumput laut E. cottonii (52,67%), lebih tinggi dan berbeda secara signifikan (p<0,05) dibandingkan teknik kimiawi (37,02%). Waktu hidrolisis terbaik yang ditemukan adalah selama tiga hari. Sementara itu, karakterisasi bioaktivitas menunjukkan bahwa hidrolisat U. lactuca memiliki potensi sebagai bahan aktif antikoagulan, tanpa adanya aktivitas sebagai antioksidan (IC50>200 ppm) maupun antibakteri (menghambat 36% Staphylococcus aureus dan Pseudomonas aeroginosa).ABSTRACTThe Ulvan in Ulva lactuca hydrolysate is a potential bioactive compound in the biopharmacology field. The hydrolysis process to obtain this class of compounds can be done in various ways, both chemically and microbiologically. The microbiological process is a potential technique because it is easier and cheaper than the chemical method. However, studies conducted with the microbiology hydrolysis technique are still very limited, especially for U. lactuca. Thus, optimation is needed to reveal an appropriate technique that produces the maximum yield. This study aimed to optimize the microbiological hydrolysis process and identify the biological activity of the hydrolyzed extract. The microbiological hydrolysis process was performed by solid and liquid techniques using microbial endophytic fungi from Enhalus plants and marine bacteria isolated from Eucheuma cottonii. The microbiology technique was compared with chemical techniques (HCl). Optimization was also carried out on the variable of hydrolysis duration (3, 6, 9, and 12 days). The results showed that yield of liquid hydrolysis method using the microbial endophytic fungi from Enhalus plants (51.67%) and marine bacteria isolated from E. cottonii (52.67%), were higher and significantly different (p<0.05) than that of chemical techniques (37.02%). Moreover, the b","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42339671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-08DOI: 10.15578/jpbkp.v15i2.727
Jurnal Pascapanen dan Bioteknologi KP
{"title":"Preface JPBKP Vol. 15 No. 2 Tahun 2020","authors":"Jurnal Pascapanen dan Bioteknologi KP","doi":"10.15578/jpbkp.v15i2.727","DOIUrl":"https://doi.org/10.15578/jpbkp.v15i2.727","url":null,"abstract":"","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44540283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-08DOI: 10.15578/jpbkp.v15i2.725
Jurnal Pascapanen dan Bioteknologi KP
{"title":"Cover Depan JPBKP Vol. 15 No. 2 Tahun 2020","authors":"Jurnal Pascapanen dan Bioteknologi KP","doi":"10.15578/jpbkp.v15i2.725","DOIUrl":"https://doi.org/10.15578/jpbkp.v15i2.725","url":null,"abstract":"","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43468238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-08DOI: 10.15578/jpbkp.v15i2.726
Jurnal Pascapanen dan Bioteknologi KP
{"title":"Cover Belakang JPBKP Vol. 15 No. 2 Tahun 2020","authors":"Jurnal Pascapanen dan Bioteknologi KP","doi":"10.15578/jpbkp.v15i2.726","DOIUrl":"https://doi.org/10.15578/jpbkp.v15i2.726","url":null,"abstract":"","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":"25 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41330856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-27DOI: 10.15578/jpbkp.v15i1.685
Jurnal Pascapanen dan Bioteknologi KP
{"title":"Cover Belakang JPBKP Vol. 15 No. 1 Tahun 2020","authors":"Jurnal Pascapanen dan Bioteknologi KP","doi":"10.15578/jpbkp.v15i1.685","DOIUrl":"https://doi.org/10.15578/jpbkp.v15i1.685","url":null,"abstract":"","PeriodicalId":31542,"journal":{"name":"Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45376674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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