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Preface JPBKP Vol. 15 No. 1 Tahun 2020 Preface JPBKP Vol. 15 No. 1 Tahun 2020
Pub Date : 2020-06-27 DOI: 10.15578/jpbkp.v15i1.686
Jurnal Pascapanen dan Bioteknologi KP
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引用次数: 0
Cover Depan JPBKP Vol. 15 No. 1 Tahun 2020 封面JPBKP第15卷第1年2020
Pub Date : 2020-06-27 DOI: 10.15578/jpbkp.v15i1.683
Jurnal Pascapanen dan Bioteknologi KP
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引用次数: 0
Preface JPBKP Vol. 14 No. 2 Tahun 2019 Preface JPBKP Vol. 14 No. 2 Tahun 2019
Pub Date : 2019-12-30 DOI: 10.15578/jpbkp.v14i2.644
Jurnal Pascapanen dan Bioteknologi KP
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引用次数: 0
Cover Belakang JPBKP Vol. 14 No. 2 Tahun 2019 JPBKP封底第14卷第2期2019年
Pub Date : 2019-12-30 DOI: 10.15578/jpbkp.v14i2.643
Jurnal Pascapanen dan Bioteknologi KP
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引用次数: 0
Cover Depan JPBKP Vol. 14 No. 2 Tahun 2019 封面德潘JPBKP第14卷第2期2019年
Pub Date : 2019-12-30 DOI: 10.15578/jpbkp.v14i2.642
Jurnal Pascapanen dan Bioteknologi KP
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引用次数: 0
Ekstraksi Unsur Hara dari Rumput Laut Sargassum sp. 从sp海藻中提取营养成分。
Pub Date : 2019-06-28 DOI: 10.15578/JPBKP.V14I1.547
Jamal Basmal, R. Saputra, Rahman Karnila, Tjipto Leksono
AbstrakPenelitian pengaruh suhu dan waktu esktraksi terhadap jumlah unsur hara makro yang ke luar dari rumput laut Sargassum sp. telah dilakukan dengan cara merendam rumput laut Sargassum sp. dalam larutan KOH 0,3% selama 5 hari, selanjutnya rumput laut diberi perlakuan waktu dan suhu ekstraksi yang berbeda yakni T1 (120 menit); T2 (180 menit); dan T3 (240 menit),  dengan suhu ekstraksi yaitu S1 (60 °C); S2 (70 °C); dan S3 (80 °C). Tujuan penelitian ini adalah untuk mendapatkan unsur hara makro dan karakteristik fisik dan mikrobiologi yang optimal dari ekstrak cair rumput laut Sargassum sp. Hasil penelitian menunjukkan bahwa perlakuan suhu dan waktu ekstraksi berpengaruh signifikan terhadap jumlah unsur hara, viskositas, angka lempeng total (ALT), fosfat,  COrganik, dan EC dari cairan ekstraksi rumput laut Sargassum sp., namun tidak berpengaruh terhadap pH, kadar nitrogen, dan kadar kaliumnya. Hasil ekstraksi terbaik ditemukan pada kombinasi perlakuan lama waktu 240 menit (4 jam) dengan suhu 80 °C, yaitu dengan jumlah larutan ekstrak yang mengandung unsur hara  sebesar 97,5%; pH 9; viskositas 3,07 cPs; ALT 2,5 x 103 Cfu/mL;  nitrogen1,64%; fosfat 197,7 ppm; kalium 205,54 ppm;  COrganik 0,55 %; EC 3,52 mS/cm dan C/N rasio 0,34%. Nutrient Extraction from Seaweed Sargassum sp. AbstractNutrient extraction from seaweed (Sargassum sp.) was  done by soaking the seaweed  in a 0.3% KOH solution during 5 days. Furthermore,  the seaweed  was  treated at various extraction times (T1 = 120 min; T2 = 180 min; and T3 = 240 min) and temperatures (S1 = 60 0C; S2 = 70 0C; and S3 = 80 0C). This study aimed to obtain optimum macro nutrients value and the physical and microbiological characteristics of the liquid extracted from seaweed Sargassum sp. The results showed that the variation of temperature and extraction time was affected significantly on the yield, viscosity, total plate count (TPC), phosphorus, Corganic, and EC of the extracted liquid from seaweed Sargassum sp., but it did not affect the pH, nitrogen and  potassium contents. The best result was found in combination of 240 min extraction time at  80 °C temperature with the yield of 97.5%; pH 9; viscosity 3.07 cPs; ALT 2.5 x 103 Cfu/mL; nitrogen 1.64%; phospat 197.7 ppm; potassium 205.54 ppm; Corganic 0.55%; EC 3.52 mS/cm and C/N ratio 0.34%.
摘要分析了提取温度和时间对马尾藻外部宏观物质量的影响。通过将马尾藻在0.3%KOH溶液中浸泡5天,然后用不同的提取时间和提取温度T1(120分钟)进行海藻提取;T2(180分钟);T3(240分钟),提取温度为S1(60°C);S2(70°C);和S3(80°C)。本研究的目的是获得海藻提取物马尾藻的最佳宏观、物理和微生物特性。研究结果表明,温度和提取时间处理显著影响海藻提取液马尾藻中海藻元素的含量、粘度、总酶(ALT)、磷酸盐和有机物以及EC。,但它不影响pH、氮和钾。最佳提取结果是在80°C下进行240分钟(4小时)的长时间处理,即提取物中天然元素含量为97.5%;pH 9;粘度3.07 cPs;ALT 2.5 x 103 Cfu/mL;[UNK]氮1.64%;磷酸盐197,7ppm;钾205,54ppm;[UNK]有机0,55%;EC 3,52 mS/cm,C/N比0,34%。从海藻马尾藻中提取营养。从海藻(马尾藻属)中提取营养是通过将海藻浸泡在0.3%的KOH溶液中5天来完成的。此外,在不同的提取时间(T1=120分钟;T2=180分钟;T3=240分钟)和温度(S1=60℃;S2=70℃;S3=80℃)下处理[UNK]海藻[UNK]。本研究旨在获得海藻马尾藻提取液的最佳宏观营养价值以及物理和微生物特性。结果表明,温度和提取时间的变化对海藻马尾草提取液的产量、粘度、总平板数(TPC)、磷、有机质和EC有显著影响。,-但不影响pH、氮和[UNK]钾含量。最佳结果是在[UNK]80°C温度下提取240min,产率为97.5%;pH 9;粘度3.07cPs;ALT 2.5 x 103 Cfu/mL;氮1.64%;磷197.7ppm;钾205,54ppm;有机质0.55%;EC为3.52mS/cm,C/N比为0.34%。
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引用次数: 1
Aktivitas Antioksidan dan Antikolesterol Ekstrak Rusip 活性抗氧化剂和提取物抗胆固醇
Pub Date : 2019-06-28 DOI: 10.15578/JPBKP.V14I1.568
Rinto Rinto, S. Lestari, Nanda Anggia Putri
AbstrakRusip merupakan produk fermentasi hasil perikanan yang dihasilkan oleh bakteri asam laktat yang mengandung peptida bioaktif. Penelitian ini bertujuan untuk mengetahui rendemen, kadar peptida, serta aktivitas antioksidan dan antikolesterol ekstrak rusip. Penelitian ini menggunakan dua jenis rusip, yaitu rusip A yang memiliki aktivitas antioksidan terbaik dan rusip B yang memiliki aktivitas antikolesterol terbaik didasarkan pada hasil penelitian sebelumnya. Ekstraksi dilakukan dengan metode maserasi tunggal menggunakan aquabides, dilanjutkan dengan fraksinasi berdasarkan perbedaan berat molekul. Uji antioksidan dilakukan dengan metode 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) dan uji antikolesterol dilakukan dengan metode penghambatan aktivitas enzim HMG-KoA reduktase. Hasil penelitian memperlihatkan fraksi F1 (berat molekul > 10 kDa) memiliki rendemen yang paling tinggi baik pada rusip A maupun rusip B dengan rendemen berturut-turut sebesar 16,61% dan 14,14%. Kadar peptida tertinggi rusip A dan rusip B terdapat pada fraksi E (ekstrak utuh) yaitu masing-masing sebesar 1,22% dan 1,25%. Aktivitas antioksidan tertinggi pada rusip A terdapat pada fraksi F3 (berat molekul < 1 kDa) dengan nilai hambatan sebesar 62,90% pada dosis 1 mg/mL dan aktivitas antikolesterol tertinggi terdapat pada fraksi F2 (berat molekul 1-10 kDa) dengan nilai inhibisi sebesar 50% pada dosis 5 mg/mL. Aktivitas antikolesterol dan antioksidan fraksi rusip tersebut tergolong rendah dibanding produk fermentasi ikan lainnya. Antioxidant and Anticholesterol Activity of Rusip ExtractAbstractRusip is a fermented product which is produced by lactic acid bacteria containing bioactive peptide. The purpose of this research was to study the yield, peptide content, antioxidant and anticholesterol of rusip extract. This study used two types of rusip, i.e. rusip  A which had the best antioxidant activity and rusip B that had the best anticholesterol activity based on the results of previous studies. Rusip was extracted by single maceration methode used aquabidest and continued with fractionation based on differences in molecular weight. Antioxidant assay was conducted using 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) methode and anticholesterol assay by inhibition of HMG-CoA reductase enzyme methode. The result showed that F1 fraction (molecular weight > 10 kDa) had the highest yield both in the rusip A and rusip B, i.e 16.61% and 14.14% respectively. The highest peptide content of the rusip A and rusip B was obtained from E fraction (whole extract), i.e. 1.22% and 1.25% respectively. The rusip fraction with highest antioxidant activity of rusip A was F3 fraction (molecular weight < 1 kDa) that had inhibition of 62.90% (concentration of 1 mg/mL) and the highest activity of anticholesterol was F2 fraction (molecular weight 1-10 kDa) with inhibition of 50% (concentration of 5 mg/mL). The antioxidant and anticholesterol activity of the rusip extract w
abstrarusip是一种含有生物活性肽的乳酸细菌产生的渔业发酵产品。本研究的目的是了解路苏普提取物中的灵敏度、肽水平、抗氧化剂和抗胆固醇活性。这项研究使用两种类型的rusip A,它有最好的抗氧化剂活性,而rusip B有最好的抗胆固醇活性是基于之前的研究结果。提取方法是用aquabides进行的,然后根据分子重量差异进行calcal3。抗氧化剂试验采用了2,2 ' -azino-bis(3-甲基苯丙胺-6-硫酸酸)和抗胆固醇测试,使用抑制苗木- koa酶分解酶的方法进行。研究表明,F1的分数(分子重量> 10 kDa)在rusip A和rusip B中具有最高的表单,其连续表单为16.61%和14.14%。rusip A和rusip B的最高多肽水平存在于E(完整提取)中,分别为1.22%和1.25%。rusip A中抗氧化的最高活性为F3(分子含量为< 1 kDa), 1毫克/mL的抑制值为62.90%,最高的抗胆固醇活性为F2(分子重量为1-10 kDa),剂量为5毫克/mL的抑制值为50%。rusip的抗胆固醇和抗氧化剂活性与其他鱼类发酵产品相比较低。体外抗氧化剂和抗胆固醇活性是一种经过乳酸、接触生物活性肽而制成的发酵细菌。这项研究的目的是研究先验、污染、抗氧化剂和抗抑郁药。这个研究用了两种类型的rusip, i.e. rusip A有最好的抗氧化剂活动,而rusip B有最好的抗胆固醇活动基于预科研究的建议。Rusip是由一种使用的aquabidest方法进行的,并根据分子重量不同的不同继续使用框架。抗氧化剂阿西诺被采用2.2 ' -azino- bus(3-甲基苯丙胺-6-硫酸)(ABTS)的方法和抗胆固醇的ascid由HMG-CoA减少酶卫理公会抑制。据报道,F1框架(分子重量> 10公里)的结果显示,在rusip A和rusip B, i. 161%和14%的尊重中,最重要的是最终的屈服。rusip A和rusip B的终极浸浸性被排除在E框架中,i. 1.22%和1.25%的尊重。和最高antioxidant活动《rusip fraction of rusip A是F3 fraction(分子重量< 1 kDa),以至于有62页。90%的抑制(1 mg / mL)的双臀和anticholesterol之最高活动是F2 fraction(分子)到10 kDa)和50%的抑制(5 mg / mL)的双臀。rusip extract的抗氧化物和抗胆固醇活性对其他fermenproducts很低。
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引用次数: 1
Kandungan Logam Berat Merkuri pada Ikan Tuna (Yellowfin dan Bigeye) dan Tuna-Like (Swordfish) Hasil Tangkapan dari Samudera Hindia dan Samudera Pasifik 金枪鱼和金枪鱼的重金属汞含量来自印度洋和太平洋
Pub Date : 2019-06-28 DOI: 10.15578/JPBKP.V14I1.572
Tri Handayani, M. S. Maarif, Etty Riani, N. Djazuli
AbstrakKomoditi tuna dan tuna-like merupakan hasil perikanan yang memiliki nilai ekonomis bagi Indonesia. Namun, logam berat dapat terakumulasi di biota ini karena posisinya sebagai top predator.  Penelitian ini menganalisis 895 data sekunder hasil pengujian merkuri yellowfin tuna (Thunnus albacares), bigeye tuna (Thunnus obesus) dan swordfish (Xiphias gladius), yang merupakan data official control oleh Badan Karantina, Pengendalian Mutu dan Keamanan Hasil Perikanan (BKIPM). Tujuan penelitian adalah untuk menganalisis kandungan merkuri yellowfin tuna, bigeye tuna dan swordfish, serta korelasinya dengan berat ikan dan menganalisis data kontaminan merkuri yellowfin tuna, bigeye tuna dan swordfish terkait pencemaran habitat perairan asal bahan baku di Samudera Hindia dan Samudera Pasifik. Metode analisis menggunakan regresi linier dan analisis t-test. Pengujian merkuri menggunakan Atomic Absorption Spectrophotometric (AAS). Hasil analisis menunjukkan bahwa kandungan merkuri sangat bervariasi antar jenis, berat dan asal perairan. Dua jenis tuna dari kedua perairan mengandung merkuri <1,0 mg/kg, kandungan merkuri yellowfin tuna berkisar antara 0,06±0,06 mg/kg hingga 0,33±0,18 mg/kg, sedangkan bigeye tuna antara 0,05±0,02 mg/kg hingga 0,33±0,14 mg/kg. Berat kedua ikan tidak berkorelasi terhadap kandungan merkuri, namun pada bigeye tuna terdapat perbedaan konsentrasi merkuri yang signifikan antara kelompok berat 1-10 kg/ekor dengan ukuran yang lebih besar (>100 kg/ekor). Sebaliknya pada swordfish, kandungan merkuri meningkat dengan penambahan berat ikan, yang berkisar antara 0,08±0,01 mg/kg hingga 1,61±0,02 mg/kg. Terdapat 34% (>50 kg) swordfish dari Samudera Hindia yang kandungan merkuri nya melebihi batas 1,0 mg/kg. Kandungan merkuri tuna dan tuna-like hasil tangkapan di Samudera Hindia lebih tinggi dibandingkan Samudera Pasifik dan berbeda nyata, sehingga dapat mengindikasikan Samudera Hindia lebih tercemar merkuri. Study on Mercury Concentrations in Tuna (Yellowfin and Bigeye) and Tuna-like (swordfish) Caught from Indian and Pacific OceansAbstractTuna and tuna-like species are considered as highly economic fish species for Indonesia. However, as a high-tropic level predatory species, tuna may accumulate heavy metal contaminants. This study analyzed 895 secondary data of mercury levels in tuna and tuna-like species, based on official control conducted by Badan Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan (BKIPM). The objectives of this study were to analyze correlations between mercury contents in yellowfin, bigeye and swordfish with fish weight and fish habitats (Indian and Pacific Oceans). Mercury was analyzed using Atomic Absorption Spectrophotometric (AAS). The data was analyzed based on linear regression and t-test. The result found that mercury concentrations varied significantly among species, weight and fish habitats. Two tuna species from two habitats, have mercury concentration of <1.0 mg/kg. Mercury in yellowfin ranged fr
摘要金枪鱼和类似金枪鱼的商品是印度尼西亚的经济资产。然而,重金属可以在这个生物群中积累,因为它是顶级捕食者。本研究分析了来自蓝鳍金枪鱼(Thunnus albacares)、大眼金枪鱼(Thunus obesus)和箭鱼(Xiphias gladius)测试的895个二级数据,这些数据是检疫局、相互管理和管理结果安全局(BKIPM)的官方控制数据。本研究的目的是分析金枪鱼黄鳍汞、大眼金枪鱼和箭鱼的含量及其与鱼类重量的相关性,并分析金枪鱼黄鳍汞、大眼金枪鱼和旗鱼的污染数据与印度洋和太平洋土著水域栖息地污染的关系。分析方法采用线性回归和t检验分析。使用原子吸收分光光度法(AAS)进行汞测试。分析结果表明,汞含量因水的类型、重量和来源而异。这两个水域的两种金枪鱼每尾含汞100公斤)。另一方面,箭鱼体内的汞含量随着体重的增加而增加,范围从0.08±0.01 mg/kg到1.61±0.02 mg/kg。印度海有34%(>50公斤)的箭鱼汞含量超过1.0毫克/公斤的限制。印度洋的金枪鱼汞含量和金枪鱼类渔获量高于太平洋,差异很大,因此可以表明印度洋的汞污染更严重。[UNK]印度洋和太平洋捕获的金枪鱼(黄鳍金枪鱼和大眼金枪鱼)和类似金枪鱼(箭鱼)中汞浓度的研究摘要金枪鱼和类似金枪鱼的物种被认为是印度尼西亚的高度经济鱼类。然而,作为一种高热带水平的捕食性物种,金枪鱼可能会积累重金属污染物。本研究根据Badan Karantina Ikan、Pengendalian Mutu和Keamanan Hasil Perikanan(BKIPM)进行的官方控制,分析了895个金枪鱼和金枪鱼类物种汞含量的二次数据。本研究的目的是分析黄鳍鱼、大眼鱼和箭鱼的汞含量与鱼类重量和鱼类栖息地(印度洋和太平洋)之间的相关性。用原子吸收分光光度法(AAS)分析汞。数据基于线性回归和t检验进行分析。结果发现,汞浓度在物种、体重和鱼类栖息地之间存在显著差异。来自两个栖息地的两种金枪鱼,汞浓度为100公斤)。相反,在箭鱼中观察到汞含量与鱼重之间有很强的关系,其中汞浓度随着鱼重的增加而增加。汞含量在0.08±0.01毫克/公斤至1.60±0.02毫克/公斤之间,从印度洋捕获的箭鱼(重量>50公斤)中有34%的汞含量超过1.0毫克/公斤。从印度洋捕获的金枪鱼和类似金枪鱼的汞浓度明显高于从太平洋捕获的金枪鱼,这可能表明印度洋的汞污染程度高于太平洋。
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引用次数: 3
Preface JPBKP Vol. 14 No. 1 Tahun 2019 JPBKP第14卷第1期2019
Pub Date : 2019-06-28 DOI: 10.15578/jpbkp.v14i1.610
Jpbkp Jpbkp
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引用次数: 0
Penapisan Mikroalga Penghasil Karotenoid serta Studi Pengaruh Stres Nitrogen dan Fosfor terhadap Produksi B-Karoten pada Mikroalga Oocystis sp. 生长胡萝卜素的微藻促进,以及研究氮和磷对生长微藻b -胡萝卜素的影响。
Pub Date : 2019-06-28 DOI: 10.15578/JPBKP.V14I1.598
Wahida Nia Elfiza, Abdi Dharma, N. Nasir
Abstrakb-karoten merupakan karotenoid yang bermanfaat sebagai antioksidan. Penelitian ini bertujuan menapis mikroalga yang berpotensi  mengandung karotenoid tinggi dan mempelajari pengaruh nitrogen dan fosfor terhadap produktivitas biomassa, kandungan pigmen fotosintesis dan b-karoten, pada mikroalga yang diisolasi dari perairan Danau Atas, Sumatra Barat. Penapisan mikroalga penghasil karotenoid dilakukan  dengan memberikan  paparan UV-A 326 nm terhadap kultur campuran mikroalga. Hasil penelitian menunjukkan bahwa 5 dari 18 spesies mampu bertahan pada proses penapisan. Pengaruh 9 jenis medium pertumbuhan dengan kriteria: tanpa NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, Bold Basalt Medium (BBM) normal (kontrol), tanpa KH2PO4, 3x KH2PO4, 5x KH2PO4,dan10x KH2PO4 terhadap mikroalga terpilih (Oocystis sp.) diamati. Hasil menunjukkan kandungan b-karoten tertinggi  ditemukan pada perlakuan 5x KH2PO4  yaitu sebesar 0,22 % dari berat kering mikroalga, dengan produktivitas biomassa 0,0015 g/mL/hari, serta kandungan klorofil a, klorofil b dan karotenoid total yaitu 7,15 µg/mL, 0,81 µg/mL dan 6,67 µg/mL. Berdasarkan penelitian ini dapat disimpulkan medium pertumbuhan dengan kandungan 5x KH2PO4 merupakan medium yang cocok bagi pertumbuhan Oocystis sp. untuk mendapatkan biomassa dengan kandungan b-karoten dan karotenoid tinggi tanpa harus menurunkan produktivitas biomassanya. Screening of Carotenoid Producing Microalgae and Study of the Effect of Nitrogen and Phosphorus Stress on the Production of b-Carotene in Microalgae Oocystis sp.Abstractb-carotene is a carotenoid that is useful as an antioxidant. Present study aims to screening high microalgae which contain carotenoids from water of Danau Atas lake (West Sumatra Province), and study the effect of nitrogen and phosphorus on biomass productivity, photosynthetic and b-carotene pigment content. Screening of carotenoid-producing microalgae was carried out by exposing UV-A 326 nm to mixed microalgae cultures. The results showed that 5 of the 18 species were able to survive in the screening process. The effect of growth medium, i.e. without NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, normal Bold Basalt Medium (BBM) (control), without KH2PO4, 3x KH2PO4, 5x KH2PO4, and 10x KH2PO4 on of selected microalgae (Oocystis sp.) was carried out. The result showed that the highest content of b-carotene of Oocystis sp. was obtained with 5x KH2PO4 which was 0.22% of the dry weight of biomass. Biomass productivity was 0.0015 g/mL/day and the chlorophyll a, chlorophyll b and total carotenoids contents were 7.15 µg/mL, 0.81 µg/mL and 6.67 µg/mL, respectively. Based on this research, 5x KH2PO4 can be concluded as a suitable medium for Oocystis sp. to obtained high b -carotene and carotenoid of Oocystis sp. without  reducing biomass productivity.
Abstrakb胡萝卜素是一种类胡萝卜素,具有抗氧化作用。本研究旨在过滤潜在的高类胡萝卜素微藻,并研究氮和磷对从西苏门答腊上湖水中分离的微藻的生物量生产力、光合作用色素和β-胡萝卜素含量的影响。类胡萝卜素衍生物的微藻过滤是通过提供针对微藻混合物培养物的UV-A 326nm的指示来进行的。研究表明,18种中有5种能够在过滤过程中存活下来。信封9种类型的生长培养基,具有标准:不含NaNO3,3x NaNO3、5x NaNO3.10x NaNO3.Bold玄武岩培养基(BBM)正常(对照),不含KH2PO4,3x KH2PO4、5x KH2PO4和10x KH2PO4对抗所选微藻(Oocystis sp.)。结果表明,5xKH2PO4中的β-胡萝卜素含量最高,即干微藻重量的0.2%,生物量为00015 g/mL/天,叶绿素a、叶绿素b和类胡萝卜素的总含量分别为7,15µg/mL、0,81µg/mL和6,67µg/mL。基于这项研究,含有5xKH2PO4的生长培养基是一种适合Oocystis sp.生长的培养基,以获得具有高β-胡萝卜素和类胡萝卜素含量的生物质,而不必降低其生物质生产率。[UNK]类胡萝卜素产生微藻的筛选和氮磷胁迫对藻体内β-胡萝卜素产生影响的研究。本研究旨在从西苏门答腊省达瑙阿塔斯湖水中筛选出含有类胡萝卜素的高含量微藻,并研究氮和磷对生物量、光合和β-胡萝卜素色素含量的影响。通过将UV-A 326 nm暴露于混合微藻培养物中来筛选产生类胡萝卜素的微藻。结果表明,在筛选过程中,18个物种中有5个能够存活。进行了生长培养基,即不含NaNO3、3x NaNO3,5x NaNO4、10x NaNO3的培养基,正常的Bold玄武岩培养基(BBM)(对照),不含KH2PO4、3x KH2PO4,5x KH2PO4和10x KH2PO4对所选微藻(Oocystis sp.)的影响。结果表明,5倍KH2PO4处理的卵孢子虫β-胡萝卜素含量最高,占生物量干重的0.22%。生物量生产力为0.0015 g/mL/天,叶绿素a、叶绿素b和类胡萝卜素总含量分别为7.15µg/mL、0.81µg/mL和6.67µg/mL。在此基础上,5xKH2PO4可以作为卵孢子虫的合适培养基,在不降低生物产量的情况下获得高b-胡萝卜素和类胡萝卜素。
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引用次数: 3
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Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan
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