Pub Date : 2019-07-31DOI: 10.24198/pjd.vol31no2.15531
E. H. Jubhari, Zulkarnain Wahid Dangkeng, Effendy Dangkeng
Introduction: Denture users with smoking habit often complain about colour changes in their dentures, especially on the base part. Polyamide microcrystalline is a thermoplastic denture base material and does not undergo many colour changes. The purpose of this study was to analyse the effect of filter cigarettes smoke exposure on the colour of polyamide microcrystalline materials. Methods: This study used posttest only control group design — Plate-Shaped sample with a size of 2 x 2 x 0.5 cm3. Nine samples as controls were immersed without exposure to the cigarette smoke, and 9 samples were immersed in artificial saliva with exposure to the filter cigarette smoke by six cycles, where one cycle was in the form of smoke exposure from one cigarette at 30-minute intervals. Cigarette exposure lasts for 21 days using a smoking simulation tool. Every seven days of exposure, colours were evaluated using a DSLR camera. The photos were then analysed with Adobe™ Photoshop in the CIELab colour space. The results were analysed afterwards by a one-way ANOVA variant test, and advance difference tests were analysed using a Post-Hoc test. Results: There was a significant colour change after exposure (p < 0.05), but the control group did not experience discolouration. The highest rate of colour change occurred in the second week, while in the third week the colour changes still occurred but tend to be stable. Conclusion: Exposure to the filter cigarette smoke changes the colour of polyamide microcrystalline denture base.Keywords: Polyamide microcrystalline, filter cigarette smoke, discolouration, denture base
简介：有吸烟习惯的义齿使用者经常抱怨他们的假牙颜色变化，尤其是底部。聚酰胺微晶是一种热塑性义齿基托材料，不会发生很多颜色变化。本研究的目的是分析过滤嘴香烟烟雾暴露对聚酰胺微晶材料颜色的影响。方法：本研究采用仅测试后的对照组设计——尺寸为2 x 2 x 0.5 cm3的板状样本。将9个样品作为对照，在不暴露于香烟烟雾的情况下浸泡，将9个样本在暴露于过滤嘴香烟烟雾的人造唾液中浸泡6个周期，其中一个周期是以30分钟间隔从一支香烟暴露烟雾的形式。使用吸烟模拟工具，香烟暴露持续21天。每暴露7天，使用单反相机对颜色进行评估。然后用Adobe对这些照片进行分析™ CIELab色彩空间中的Photoshop。随后通过单因素方差分析变异检验对结果进行分析，并使用Post Hoc检验对提前差异检验进行分析。结果：接触后有明显的颜色变化（p<0.05），但对照组没有变色。最高的颜色变化发生在第二周，而在第三周，颜色变化仍然发生，但趋于稳定。结论：暴露于过滤嘴香烟烟雾会改变聚酰胺微晶义齿基托的颜色。关键词：聚酰胺微晶、过滤嘴烟、变色、义齿基托
{"title":"Discolouration of polyamide microcrystalline denture base plates after exposure to filter cigarette smoke","authors":"E. H. Jubhari, Zulkarnain Wahid Dangkeng, Effendy Dangkeng","doi":"10.24198/pjd.vol31no2.15531","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.15531","url":null,"abstract":"Introduction: Denture users with smoking habit often complain about colour changes in their dentures, especially on the base part. Polyamide microcrystalline is a thermoplastic denture base material and does not undergo many colour changes. The purpose of this study was to analyse the effect of filter cigarettes smoke exposure on the colour of polyamide microcrystalline materials. Methods: This study used posttest only control group design — Plate-Shaped sample with a size of 2 x 2 x 0.5 cm3. Nine samples as controls were immersed without exposure to the cigarette smoke, and 9 samples were immersed in artificial saliva with exposure to the filter cigarette smoke by six cycles, where one cycle was in the form of smoke exposure from one cigarette at 30-minute intervals. Cigarette exposure lasts for 21 days using a smoking simulation tool. Every seven days of exposure, colours were evaluated using a DSLR camera. The photos were then analysed with Adobe™ Photoshop in the CIELab colour space. The results were analysed afterwards by a one-way ANOVA variant test, and advance difference tests were analysed using a Post-Hoc test. Results: There was a significant colour change after exposure (p < 0.05), but the control group did not experience discolouration. The highest rate of colour change occurred in the second week, while in the third week the colour changes still occurred but tend to be stable. Conclusion: Exposure to the filter cigarette smoke changes the colour of polyamide microcrystalline denture base.Keywords: Polyamide microcrystalline, filter cigarette smoke, discolouration, denture base","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48708852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.24198/pjd.vol31no2.21155
A. Azhari, G. Gianto, S. Suprijanto
Introduction: The use of panoramic x-ray radiographs with analogue film negatives is still widely used. Contrast differences in the radiograph are determined by the level of light transmitted from the viewer to the film negative and visual perception of the radiologist, which can cause differences and errors of interpretation. This study was aimed to compare the quality of scanners produced by the transmission and reflection modes objectively with direct digital imaging standard. Methods: This study used a descriptive comparative design with purposive sampling method. Radiographic analysis was conducted towards 30 analogue radiographs of Radiology laboratory of Universitas Padjadjaran Dental Hospital. Digitisation was performed using a scanner with setting modes (reflection and transmission, 8-bit intensity resolution and spatial resolution of 800 dpi). Digitising the negative radiographs of analogue films into digital radiographs will get a range of percentage pixels. Histogram of radiographs with a percentage of pixels in the grey level range 0 - 64 exceeds 50%, including "under-exposed", in the grey level range 192 - 255 exceeding 50%, including "over-exposed". The evaluation was carried out to observe the radiograph exposure homogeneity by dividing the radiograph into 4 parts. Each part was calculated as the mean pixel value; then the standard deviation was searched for the four mean values (STDMI - standard deviation mean intensity) determined the homogeneity. Results: Digitising type of radiograph reflection was included under-exposed because the percentage of pixels in the grey level range 0 - 64 was 86.94%. STDMI consecutive reflection = 8.03, transmission = 18.05, direct digital imaging = 20.86. Conclusion: Digitisation with scanner transmission mode is the best result objectively because it approaches the quality value of direct digital imaging standard.Keywords: Objective quality, homogeneity, reflection, transmission
{"title":"Comparison of the quality of the result of digitising panoramic analogue films using transmission and reflection scanner mode with direct digital imaging standard","authors":"A. Azhari, G. Gianto, S. Suprijanto","doi":"10.24198/pjd.vol31no2.21155","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.21155","url":null,"abstract":"Introduction: The use of panoramic x-ray radiographs with analogue film negatives is still widely used. Contrast differences in the radiograph are determined by the level of light transmitted from the viewer to the film negative and visual perception of the radiologist, which can cause differences and errors of interpretation. This study was aimed to compare the quality of scanners produced by the transmission and reflection modes objectively with direct digital imaging standard. Methods: This study used a descriptive comparative design with purposive sampling method. Radiographic analysis was conducted towards 30 analogue radiographs of Radiology laboratory of Universitas Padjadjaran Dental Hospital. Digitisation was performed using a scanner with setting modes (reflection and transmission, 8-bit intensity resolution and spatial resolution of 800 dpi). Digitising the negative radiographs of analogue films into digital radiographs will get a range of percentage pixels. Histogram of radiographs with a percentage of pixels in the grey level range 0 - 64 exceeds 50%, including \"under-exposed\", in the grey level range 192 - 255 exceeding 50%, including \"over-exposed\". The evaluation was carried out to observe the radiograph exposure homogeneity by dividing the radiograph into 4 parts. Each part was calculated as the mean pixel value; then the standard deviation was searched for the four mean values (STDMI - standard deviation mean intensity) determined the homogeneity. Results: Digitising type of radiograph reflection was included under-exposed because the percentage of pixels in the grey level range 0 - 64 was 86.94%. STDMI consecutive reflection = 8.03, transmission = 18.05, direct digital imaging = 20.86. Conclusion: Digitisation with scanner transmission mode is the best result objectively because it approaches the quality value of direct digital imaging standard.Keywords: Objective quality, homogeneity, reflection, transmission","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47432426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.24198/pjd.vol31no2.22863
Dias Mareta Kusumaningrum, S. Tjahajawati, Ervin Rizali
Introduction: Salivary pH, viscosity and volume play an important role in maintaining tooth and oral tissue integrity. The purpose of this research was to evaluate the difference of young adult smokers and non-smokers saliva reviewed by its pH, viscosity and volume. Methods: The research sample of 28 smokers and 24 non-smokers. Saliva was collected by spitting method then the pH, viscosity and volume was measured. The data was analyze using independent sample t-test and chi-square. Results: The result indicated that there was a significant difference in the mean value of salivary pH on smokers and non-smokers with p-value 0.000 (p < 0.05). There was a significant difference in the mean value of salivary viscosity on smokers and non-smokers with p-value 0.000 (p < 0.05.) There was a significant difference in the mean value of salivary volume on smokers and non-smokers with p-value 0.000 (p < 0.05). Conclusion: The conclusion of the research showed that salivary pH of smokers was lower than non-smokers, salivary viscosity of smokers was higher than non-smokers and salivary volume of smokers was fewer than non-smokers.Keywords: Young adult, smokers, non-smokers, saliva, salivary pH, salivary viscosity, salivary volume
{"title":"Differences of young adult smokers and non-smokers saliva reviewed by salivary pH, viscosity, and volume","authors":"Dias Mareta Kusumaningrum, S. Tjahajawati, Ervin Rizali","doi":"10.24198/pjd.vol31no2.22863","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.22863","url":null,"abstract":"Introduction: Salivary pH, viscosity and volume play an important role in maintaining tooth and oral tissue integrity. The purpose of this research was to evaluate the difference of young adult smokers and non-smokers saliva reviewed by its pH, viscosity and volume. Methods: The research sample of 28 smokers and 24 non-smokers. Saliva was collected by spitting method then the pH, viscosity and volume was measured. The data was analyze using independent sample t-test and chi-square. Results: The result indicated that there was a significant difference in the mean value of salivary pH on smokers and non-smokers with p-value 0.000 (p < 0.05). There was a significant difference in the mean value of salivary viscosity on smokers and non-smokers with p-value 0.000 (p < 0.05.) There was a significant difference in the mean value of salivary volume on smokers and non-smokers with p-value 0.000 (p < 0.05). Conclusion: The conclusion of the research showed that salivary pH of smokers was lower than non-smokers, salivary viscosity of smokers was higher than non-smokers and salivary volume of smokers was fewer than non-smokers.Keywords: Young adult, smokers, non-smokers, saliva, salivary pH, salivary viscosity, salivary volume","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48473260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.24198/pjd.vol31no2.17956
Chrisni Oktavia Jusup, E. S. Soemantri, Endah Mardiati, I. A. Evangelina
Introduction: The base surface roughness of slot bracket can affect friction in tooth movement. There are ISO-certified and non-ISO-certified stainless steel brackets on the market. Thus orthodontists must be careful in choosing the right bracket. This study was aimed to evaluate the differences of the surface roughness of ISO-certified and non-ISO-certified Roth 0.022 inch stainless steel bracket with the parameter of S a (average roughness). Methods: This research was a laboratory observational. Samples were taken randomly as many as 32 brackets divided into two groups, each consisted of two bracket brands. Surface roughness measurements were performed using an atomic force microscope (AFM), then the data were analysed by ANOVA test (p < 0.05) and Post-Hoc analysis. Results: The ISO-certified bracket has an S a value smaller than the non-ISO-certified bracket. There was a significant difference in the surface roughness of the ISO-certified and non-ISO-certified slot bracket base (p < 0.05). Conclusion: The ISO-certified bracket has a smoother base surface than the non-ISO-certified bracket base.Keywords: Surface roughness, stainless steel slot bracket, atomic force microscope
{"title":"Evaluation of the basic surface roughness of an ISO-certified and non-ISO-certified slot bracket with an atomic force microscope","authors":"Chrisni Oktavia Jusup, E. S. Soemantri, Endah Mardiati, I. A. Evangelina","doi":"10.24198/pjd.vol31no2.17956","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.17956","url":null,"abstract":"Introduction: The base surface roughness of slot bracket can affect friction in tooth movement. There are ISO-certified and non-ISO-certified stainless steel brackets on the market. Thus orthodontists must be careful in choosing the right bracket. This study was aimed to evaluate the differences of the surface roughness of ISO-certified and non-ISO-certified Roth 0.022 inch stainless steel bracket with the parameter of S a (average roughness). Methods: This research was a laboratory observational. Samples were taken randomly as many as 32 brackets divided into two groups, each consisted of two bracket brands. Surface roughness measurements were performed using an atomic force microscope (AFM), then the data were analysed by ANOVA test (p < 0.05) and Post-Hoc analysis. Results: The ISO-certified bracket has an S a value smaller than the non-ISO-certified bracket. There was a significant difference in the surface roughness of the ISO-certified and non-ISO-certified slot bracket base (p < 0.05). Conclusion: The ISO-certified bracket has a smoother base surface than the non-ISO-certified bracket base.Keywords: Surface roughness, stainless steel slot bracket, atomic force microscope","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48997016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.24198/pjd.vol31no2.15362
Ira Artilia
Introduction: Gypsum, Portland, and apatite cement, and gypsum have been developed as dental materials for a long time. Similarity of the three materials leads to some relevant approaches to improve the characteristic of dental materials. Water reducing agent is commonly used in detergent or soap and also used as a conventional method in the cement industry to increase the handling and mechanical property. Cement paste mixed with water reducing agent is more flowable (ease handling), and the set mass is harder (mechanical property increased). However, it has a problem with biomaterial cement due to the biocompatibility. This water reducing agent is harmful to living tissue. Therefore, a new approach will be demonstrated in this research. Methods: In this study, the hydrophilicity of cement powder was modified by ozone gas treatment. Ozone gas treatment will be applied to modify the hydrophilicity of cement particles; therefore, it can act similar to the water reducing agent. Results: The hydrophilicity of gypsum, Portland, and apatite cement powder was significantly increased after ozone gas treatment. The hydrophilicity improvement of cement powder increased the ability of water to interact with the cement powder. The benefit is the flowability improvement of cement paste. Therefore, the manipulation index would also be increased. The mechanical property would be increased because the water added for manipulation was decreasing. Conclusion: Ozone gas treatment could improve the hydrophilicity of gypsum, Portland, and apatite cement powder.Keywords: Gypsum, Portland, apatite cement, hydrophilic, ozone
{"title":"Modification of surface hydrophilicity of dental materials by ozone","authors":"Ira Artilia","doi":"10.24198/pjd.vol31no2.15362","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.15362","url":null,"abstract":"Introduction: Gypsum, Portland, and apatite cement, and gypsum have been developed as dental materials for a long time. Similarity of the three materials leads to some relevant approaches to improve the characteristic of dental materials. Water reducing agent is commonly used in detergent or soap and also used as a conventional method in the cement industry to increase the handling and mechanical property. Cement paste mixed with water reducing agent is more flowable (ease handling), and the set mass is harder (mechanical property increased). However, it has a problem with biomaterial cement due to the biocompatibility. This water reducing agent is harmful to living tissue. Therefore, a new approach will be demonstrated in this research. Methods: In this study, the hydrophilicity of cement powder was modified by ozone gas treatment. Ozone gas treatment will be applied to modify the hydrophilicity of cement particles; therefore, it can act similar to the water reducing agent. Results: The hydrophilicity of gypsum, Portland, and apatite cement powder was significantly increased after ozone gas treatment. The hydrophilicity improvement of cement powder increased the ability of water to interact with the cement powder. The benefit is the flowability improvement of cement paste. Therefore, the manipulation index would also be increased. The mechanical property would be increased because the water added for manipulation was decreasing. Conclusion: Ozone gas treatment could improve the hydrophilicity of gypsum, Portland, and apatite cement powder.Keywords: Gypsum, Portland, apatite cement, hydrophilic, ozone","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44289500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.24198/pjd.vol31no2.18995
Yenni Hendriani Praptiwi, Natanael Dwi Prayitno, S. Sukmasari
Introduction: Molar Incisor Hypomineralisation (MIH) is an enamel defect with white, yellow or black colour due to minerals insufficient during tooth development. The abnormality of prismatic enamel structures and tooth hypersensitivity on patients with MIH may disturb the success rate of restoration. The objective of this research was to determine the prevalence of MIH in primary school children. Methods: A cross-sectional study with purposive sampling on 619 primary school children at Pasteur Urban Village of Bandung City, Indonesia. All dentition were scored using the European Association of Paediatric Dentist (EAPD) MIH scoring sheet, and oral hygiene status assessment with simplified OHI. All data were descriptively described using distribution table. Results: Distribution of MIH on total of 619 children were scored as follows: 0,1,1a, 2, 2a, 3, 4, 5, 6, 7, 8, 9,10 (0 = free enamel defect; 6, 7, and 8 = alteration to non MIH; 1 -5 and 9 -10 = MIH) with frequencies 417(67.36%), 39(6.30%), 60(.65%), 13(2.10%), 0(0.00%), 7(1.13%), 0(0.00%), 4(0.65%), 0(0.00%), 26(4.20%), 58(9.36%), 0(0.00%), and 49(7.91%) respectively. Conclusion: Prevalence of children with MIH in primary school is quite high (19% (118 out of 619 participants)). The highest prevalence is found at the 11 years old group with 49 participants (36.84%), while the prevalence in boys (83 (62.4%)) is found to be higher than girls (50 (37.59%)).Keywords: Prevalence, Molar Incisor Hypomineralisation (MIH), primary school children
{"title":"Prevalence of Molar Incisors Hypomineralisation (MIH) in primary school children","authors":"Yenni Hendriani Praptiwi, Natanael Dwi Prayitno, S. Sukmasari","doi":"10.24198/pjd.vol31no2.18995","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.18995","url":null,"abstract":"Introduction: Molar Incisor Hypomineralisation (MIH) is an enamel defect with white, yellow or black colour due to minerals insufficient during tooth development. The abnormality of prismatic enamel structures and tooth hypersensitivity on patients with MIH may disturb the success rate of restoration. The objective of this research was to determine the prevalence of MIH in primary school children. Methods: A cross-sectional study with purposive sampling on 619 primary school children at Pasteur Urban Village of Bandung City, Indonesia. All dentition were scored using the European Association of Paediatric Dentist (EAPD) MIH scoring sheet, and oral hygiene status assessment with simplified OHI. All data were descriptively described using distribution table. Results: Distribution of MIH on total of 619 children were scored as follows: 0,1,1a, 2, 2a, 3, 4, 5, 6, 7, 8, 9,10 (0 = free enamel defect; 6, 7, and 8 = alteration to non MIH; 1 -5 and 9 -10 = MIH) with frequencies 417(67.36%), 39(6.30%), 60(.65%), 13(2.10%), 0(0.00%), 7(1.13%), 0(0.00%), 4(0.65%), 0(0.00%), 26(4.20%), 58(9.36%), 0(0.00%), and 49(7.91%) respectively. Conclusion: Prevalence of children with MIH in primary school is quite high (19% (118 out of 619 participants)). The highest prevalence is found at the 11 years old group with 49 participants (36.84%), while the prevalence in boys (83 (62.4%)) is found to be higher than girls (50 (37.59%)).Keywords: Prevalence, Molar Incisor Hypomineralisation (MIH), primary school children","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41667501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-18DOI: 10.24198/pjd.vol31no2.18540
Zakiyya Ulpiyah, A. D. Shita, M. A. Wahyukundari
Introduction: Porphyromonas gingivalis growth should be prevented to minimise inflammation in periodontal tissues. Antibacterial herbs need to be considered because there are side effects caused by synthetic antibacterial drugs. Namnam (Cynometra cauliflora L.) leaves are known for having antibacterial effects. The purpose of this research was analysing the inhibition potential, and the highest inhibition concentration of namnam leaves extract on the growth of Porphyromonas gingivalis. Methods: 24 samples were divided into 6 groups. The positive control group was given 0.2% chlorhexidine, and the treatment group was given various concentrations (100%, 80%, 60%, 40%, and 20%) of namnam leaves extract. The disc which spilled by various concentrations of namnam leaves extracts and 0.2% chlorhexidine was placed on a medium which has been inoculated by P. gingivalis, then incubated at 37ºC for 48 hours. The inhibition zone was measured using a vernier calliper. Results: The concentration of 100% had the highest average inhibition zone value, which was 11.43 mm. The content in namnam leaves extracts which serve as antibacterial were tannins, flavonoids, triterpenoids, saponins and quinones. Conclusion: Namnam leaves extract can inhibit the growth of P. gingivalis. 100% of namnam leaves extract has the highest antibacterial inhibition zone.Keywords: Antibacterial, Namnam leaves extract, periodontal disease, Porphyromonas gingivalis
{"title":"Inhibition of namnam (Cynometra cauliflora L.) leaves extract on the growth of Porphyromonas gingivalis","authors":"Zakiyya Ulpiyah, A. D. Shita, M. A. Wahyukundari","doi":"10.24198/pjd.vol31no2.18540","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.18540","url":null,"abstract":"Introduction: Porphyromonas gingivalis growth should be prevented to minimise inflammation in periodontal tissues. Antibacterial herbs need to be considered because there are side effects caused by synthetic antibacterial drugs. Namnam (Cynometra cauliflora L.) leaves are known for having antibacterial effects. The purpose of this research was analysing the inhibition potential, and the highest inhibition concentration of namnam leaves extract on the growth of Porphyromonas gingivalis. Methods: 24 samples were divided into 6 groups. The positive control group was given 0.2% chlorhexidine, and the treatment group was given various concentrations (100%, 80%, 60%, 40%, and 20%) of namnam leaves extract. The disc which spilled by various concentrations of namnam leaves extracts and 0.2% chlorhexidine was placed on a medium which has been inoculated by P. gingivalis, then incubated at 37ºC for 48 hours. The inhibition zone was measured using a vernier calliper. Results: The concentration of 100% had the highest average inhibition zone value, which was 11.43 mm. The content in namnam leaves extracts which serve as antibacterial were tannins, flavonoids, triterpenoids, saponins and quinones. Conclusion: Namnam leaves extract can inhibit the growth of P. gingivalis. 100% of namnam leaves extract has the highest antibacterial inhibition zone.Keywords: Antibacterial, Namnam leaves extract, periodontal disease, Porphyromonas gingivalis","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44496214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Oral ulcer lesions are one of the most common lesions in the oral cavity. The prevalence of traumatic ulcers is quite high compared to other oral lesions. Brown algae, Sargassum sp. is one of the algae contains flavonoids, vitamin A, vitamin C, iron, and calcium, which play a role in wound healing and has economic value. This study was aimed to determine the effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus). Methods: 28 Wistar strain (Rattus norvegicus) weighed 150-200 grams. The samples were divided into 4 groups (K1, K2, P1, P2); each group consisted of 7 mice. K1 group was administered with Hydroxy Propyl Methyl Cellulose (HPMC), K2 was administered with 0.2% hyaluronic acid gel, P1 was administered with 50% Sargassum sp. extract gel, and P2 was administered with 75% Sargassum sp. extract gel. In each group, the ulcer was made on the mice labial mucosa; then the ulcer diameter was measured and observed on day 1 and day 7. Results: The mean diameter ± standard deviation of traumatic ulcer calculations were K1 (1.09 ± 0.40), K2 (1.81 ± 0.67), P1 (1.39 ± 0.43) and P2 (2.16 ± 0.49) respectively. One way ANOVA test results showed significant differences in K1, K2, P1, and P2 groups. Conclusion: Ethanol extract of Sargassum sp. is effective in accelerating the traumatic ulcers healing at the concentration of 75%.Keywords: Traumatic ulcer, Sargassum sp., wound healing.
{"title":"Effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus)","authors":"Annisya Ristie Arwidasari, Twi Agnita Cevanti, Isidora Karsini Soewondo","doi":"10.24198/pjd.vol31no1.16513","DOIUrl":"https://doi.org/10.24198/pjd.vol31no1.16513","url":null,"abstract":"Introduction: Oral ulcer lesions are one of the most common lesions in the oral cavity. The prevalence of traumatic ulcers is quite high compared to other oral lesions. Brown algae, Sargassum sp. is one of the algae contains flavonoids, vitamin A, vitamin C, iron, and calcium, which play a role in wound healing and has economic value. This study was aimed to determine the effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus). Methods: 28 Wistar strain (Rattus norvegicus) weighed 150-200 grams. The samples were divided into 4 groups (K1, K2, P1, P2); each group consisted of 7 mice. K1 group was administered with Hydroxy Propyl Methyl Cellulose (HPMC), K2 was administered with 0.2% hyaluronic acid gel, P1 was administered with 50% Sargassum sp. extract gel, and P2 was administered with 75% Sargassum sp. extract gel. In each group, the ulcer was made on the mice labial mucosa; then the ulcer diameter was measured and observed on day 1 and day 7. Results: The mean diameter ± standard deviation of traumatic ulcer calculations were K1 (1.09 ± 0.40), K2 (1.81 ± 0.67), P1 (1.39 ± 0.43) and P2 (2.16 ± 0.49) respectively. One way ANOVA test results showed significant differences in K1, K2, P1, and P2 groups. Conclusion: Ethanol extract of Sargassum sp. is effective in accelerating the traumatic ulcers healing at the concentration of 75%.Keywords: Traumatic ulcer, Sargassum sp., wound healing.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43596825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-31DOI: 10.24198/pjd.vol31no1.16950
Z. Meilawaty, Agustin Wulan Suci Dharmayanti, Dinar Prafitasari
{"title":"COX-2 expression in neutrophils treated cassava leaf extract (Manihot Esculenta) and lipopolysaccharide (LPS) of E. coli (in vitro study)","authors":"Z. Meilawaty, Agustin Wulan Suci Dharmayanti, Dinar Prafitasari","doi":"10.24198/pjd.vol31no1.16950","DOIUrl":"https://doi.org/10.24198/pjd.vol31no1.16950","url":null,"abstract":"","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69269076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-31DOI: 10.24198/pjd.vol31no1.21236
Anindhita Cindy Felicia, V. Sugiaman, Natallia Pranata
Introduction: Various active compounds of herbal plants that are known to accelerate the wound healing process are flavonoids, tannins, and saponins. One amongst them is clove (Syzygium aromaticum), with the leaves as the most common part to be used as herbal medicine due to the antibacterial, anti-inflammatory, and antioxidant properties. However, the use of the biocompatibility of clove leaves as herbal medicine is still unknown. Therefore, this study was aimed to determine the cytotoxicity effects of clove leaves ethanol extract on the fibroblast cells and safe dosage (IC50) for medicinal use. Methods: Phytochemical tests using the Farnsworth method and viability test was performed using the laboratory experimental with post-test only control group design. The samples were divided into seven concentrations; 500, 250, 125, 62.5, 31.25, 15.63, and 7.81 μg/ml, with three repetitions (triplo) towards the fibroblast cells. Viability test was performed using MTS assay. Results: Based on the results obtained from one-way ANOVA statistical test, there was a significant difference between the percentage of cell viability and each concentration (α ≤ 0.05), with IC50 value of 344.814 μg/ml. Conclusion: Clove (Syzygium aromaticum) leaves ethanol extract showed a high percentage of cell viability at the concentrations below the IC50 value.Keywords: Clove leaves, cytotoxicity, fibroblast cell, MTS assay, periodontal diseases, viability.
{"title":"Viability of fibroblast cells on the clove (Syzygium aromaticum) leaves ethanol extract","authors":"Anindhita Cindy Felicia, V. Sugiaman, Natallia Pranata","doi":"10.24198/pjd.vol31no1.21236","DOIUrl":"https://doi.org/10.24198/pjd.vol31no1.21236","url":null,"abstract":"Introduction: Various active compounds of herbal plants that are known to accelerate the wound healing process are flavonoids, tannins, and saponins. One amongst them is clove (Syzygium aromaticum), with the leaves as the most common part to be used as herbal medicine due to the antibacterial, anti-inflammatory, and antioxidant properties. However, the use of the biocompatibility of clove leaves as herbal medicine is still unknown. Therefore, this study was aimed to determine the cytotoxicity effects of clove leaves ethanol extract on the fibroblast cells and safe dosage (IC50) for medicinal use. Methods: Phytochemical tests using the Farnsworth method and viability test was performed using the laboratory experimental with post-test only control group design. The samples were divided into seven concentrations; 500, 250, 125, 62.5, 31.25, 15.63, and 7.81 μg/ml, with three repetitions (triplo) towards the fibroblast cells. Viability test was performed using MTS assay. Results: Based on the results obtained from one-way ANOVA statistical test, there was a significant difference between the percentage of cell viability and each concentration (α ≤ 0.05), with IC50 value of 344.814 μg/ml. Conclusion: Clove (Syzygium aromaticum) leaves ethanol extract showed a high percentage of cell viability at the concentrations below the IC50 value.Keywords: Clove leaves, cytotoxicity, fibroblast cell, MTS assay, periodontal diseases, viability.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46455020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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