Brian Enrique Rojo-Ruvalcaba, T. García-Cobián, S. Pascoe-González, T. I. Campos-Bayardo, Luz María Guzmán-García, María Cristina Gil-Gálvez, Zyanya Escobar-Millán, Eduardo Huerta-García, T. Garcia-Iglesias
: Uncontrolled proliferation and death resistance are two hallmarks of cancer cells. Breast cancer (BC) has the highest incidence and mortality in women worldwide and national; subtype triple negative (TN) is the most aggressive type because it is not susceptible to conventional therapy. Since 2004, the World Health Organization (WHO) approved use of alternative treatments as adjuvants based on evidence of its benefits. Since then, different natural alternatives to treat cancer have been studied, including Origanum vulgare ( Ov ) and carvacrol (Crv), one of its main compounds. However, cytotoxic potential of these products has not been studied in this subtype of BC. The objective of this study was to evaluate the cytotoxic effect of Ov and Crv on TN BC cell line (HCC-70). Lethal dose 50 was determined on control cell line HaCaT through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays with Ov and Crv stimulus in different doses and concentrations; the found dose was used on the HCC-70 cell line. The results were evaluated by ANOVA, finding that Ov reduced the proliferation by 94.05% ± 0.11 ( p < 0.001) and Crv by 93.43% ± 0.21 ( p < 0.001) compared to untreated cells. This suggests that both Ov and Crv present a powerful cytotoxic effect against the HCC-70 cell line and are promising options that should receive further study.
{"title":"DOSE-DEPENDENT CYTOTOXICITY OF THE ORIGANUM VULGARE AND CARVACROL ON TRIPLE NEGATIVE BREAST CANCER CELL LINE (HCC-70).","authors":"Brian Enrique Rojo-Ruvalcaba, T. García-Cobián, S. Pascoe-González, T. I. Campos-Bayardo, Luz María Guzmán-García, María Cristina Gil-Gálvez, Zyanya Escobar-Millán, Eduardo Huerta-García, T. Garcia-Iglesias","doi":"10.3390/iecn2020-07000","DOIUrl":"https://doi.org/10.3390/iecn2020-07000","url":null,"abstract":": Uncontrolled proliferation and death resistance are two hallmarks of cancer cells. Breast cancer (BC) has the highest incidence and mortality in women worldwide and national; subtype triple negative (TN) is the most aggressive type because it is not susceptible to conventional therapy. Since 2004, the World Health Organization (WHO) approved use of alternative treatments as adjuvants based on evidence of its benefits. Since then, different natural alternatives to treat cancer have been studied, including Origanum vulgare ( Ov ) and carvacrol (Crv), one of its main compounds. However, cytotoxic potential of these products has not been studied in this subtype of BC. The objective of this study was to evaluate the cytotoxic effect of Ov and Crv on TN BC cell line (HCC-70). Lethal dose 50 was determined on control cell line HaCaT through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays with Ov and Crv stimulus in different doses and concentrations; the found dose was used on the HCC-70 cell line. The results were evaluated by ANOVA, finding that Ov reduced the proliferation by 94.05% ± 0.11 ( p < 0.001) and Crv by 93.43% ± 0.21 ( p < 0.001) compared to untreated cells. This suggests that both Ov and Crv present a powerful cytotoxic effect against the HCC-70 cell line and are promising options that should receive further study.","PeriodicalId":320592,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease","volume":"262 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121426354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Correa-Rodríguez, G. Pocovi-Gerardino, Irene Medina-Martínez, Sara Del Olmo-Romero, N. Ortego-Centeno, B. Rueda-Medina
: Systemic lupus erythematosus (SLE) is a chronic disease characterized by inflammatory response and abnormal autoimmune disease. Vitamin D is essential in phosphorus-calcium metabolism, has immunosuppressive properties, and is considered a therapeutic option. Controversy exists about the role of this vitamin in the pathogenesis of SLE. Thus, the aim of this study was to investigate the influence of the dietary intake of vitamin D and its supplementation in a cohort of patients with SLE. A cross-sectional study including a total 285 patients with SLE was conducted (248 females and 26 males; mean age 46.99 ± 12.89 years). The SLE disease activity index (SLEDAI-2K) and the SLICC/ACR damage index (SDI) were used to assess disease activity and disease-related damage, respectively. Levels of C-reactive protein (CRP; mg/dL), homocysteine (Hcy; mol/L), anti-double stranded DNA antibodies (anti-dsDNA) (IU/mL), complement C3 (mg/dL), and complement C4 (mg/dL), among other biochemical markers, were measured. The dietary intake of vitamin D and the intake of vitamin D supplement were obtained via a 24-h patient diary. A share of 57.1% of the patients took vitamin D supplements and the average of dietary vitamin D was 2.08 ± 2.94 μ g/day. Note that 98.2% of patients did not reach the recommended dietary intakes for vitamin D intake. Multivariate regression analysis revealed that clinical and laboratory variables are not significantly affected by vitamin D intake levels after adjusting for age, gender, energy intake, and medical treatment (immunosuppressants, corticosteroids, and antimalarials). Patients with SLE who took vitamin D supplements had significantly higher serum complement C3 levels compared to patients who did not take them after adjusting for covariates (110.28 ± 30.93 vs. 107.38 ± 24.18; p = 0.018). Our findings suggest a potential impact of supplementation of vitamin D on the activity of SLE. Future longitudinal research on SLE patients, including intervention trials, are required to validate these preliminary data.
{"title":"Vitamin D supplementation is association with disease activity in systemic lupus erythematosus patients","authors":"María Correa-Rodríguez, G. Pocovi-Gerardino, Irene Medina-Martínez, Sara Del Olmo-Romero, N. Ortego-Centeno, B. Rueda-Medina","doi":"10.3390/iecn2020-07013","DOIUrl":"https://doi.org/10.3390/iecn2020-07013","url":null,"abstract":": Systemic lupus erythematosus (SLE) is a chronic disease characterized by inflammatory response and abnormal autoimmune disease. Vitamin D is essential in phosphorus-calcium metabolism, has immunosuppressive properties, and is considered a therapeutic option. Controversy exists about the role of this vitamin in the pathogenesis of SLE. Thus, the aim of this study was to investigate the influence of the dietary intake of vitamin D and its supplementation in a cohort of patients with SLE. A cross-sectional study including a total 285 patients with SLE was conducted (248 females and 26 males; mean age 46.99 ± 12.89 years). The SLE disease activity index (SLEDAI-2K) and the SLICC/ACR damage index (SDI) were used to assess disease activity and disease-related damage, respectively. Levels of C-reactive protein (CRP; mg/dL), homocysteine (Hcy; mol/L), anti-double stranded DNA antibodies (anti-dsDNA) (IU/mL), complement C3 (mg/dL), and complement C4 (mg/dL), among other biochemical markers, were measured. The dietary intake of vitamin D and the intake of vitamin D supplement were obtained via a 24-h patient diary. A share of 57.1% of the patients took vitamin D supplements and the average of dietary vitamin D was 2.08 ± 2.94 μ g/day. Note that 98.2% of patients did not reach the recommended dietary intakes for vitamin D intake. Multivariate regression analysis revealed that clinical and laboratory variables are not significantly affected by vitamin D intake levels after adjusting for age, gender, energy intake, and medical treatment (immunosuppressants, corticosteroids, and antimalarials). Patients with SLE who took vitamin D supplements had significantly higher serum complement C3 levels compared to patients who did not take them after adjusting for covariates (110.28 ± 30.93 vs. 107.38 ± 24.18; p = 0.018). Our findings suggest a potential impact of supplementation of vitamin D on the activity of SLE. Future longitudinal research on SLE patients, including intervention trials, are required to validate these preliminary data.","PeriodicalId":320592,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115092862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Grain legumes, which are commonly referred to as pulses, are staple foods in many parts of the world, but are infrequently consumed in most economically developed countries where the obesity pandemic is prominent. However, even in low pulse consuming countries such as the United States, there are sub-groups of individuals who consume large amounts of pulses. Systematic reviews of population studies indicate that pulse consumers have a lower risk for developing obesity. To determine whether these population-based findings could be modeled in preclinical systems in which such findings can be deconstructed, we used rat and mouse models of dietary induced obesity and reported that lipid accumulation was inhibited. In this study, we examined the relationship between inhibition of fat accumulation and changes in the gut associated microbiome in male C57BL/6 mice fed either a high fat diet with casein as the protein source or that diet formulation in which one of four pulses (chickpea, common bean, dry pea, or lentil) was substituted to provide 70% dietary protein with the remainder provided by casein. The seeds of each pulse were soaked, cooked, and then freeze-dried and milled; the resulting powder was used for diet formulation. Mice were ad libitum fed over the 17-week duration of the feeding trial. Cecal content was obtained at necropsy and immediately snap frozen in liquid nitrogen. Extracted genomic DNA was processed for 16s rRNA sequencing on an Illumina system. Significant differences were observed between each pulse and the high fat control diet in microbial phylogenetic diversity (p < 0.001) and accumulation of lipid in adipose depots (p < 0.01). Differences among pulses were also observed in both metrics. Microbiome alpha and beta diversity metrics, differences in abundance for each detected taxon among treatment groups, and their relationships to changes in lipid accumulation in adipose storage depots are reported.
{"title":"Effect of pulse consumption on obesity and the metagenome","authors":"H. Thompson, T. Weir, J. McGinley, E. Neil","doi":"10.3390/iecn2020-07009","DOIUrl":"https://doi.org/10.3390/iecn2020-07009","url":null,"abstract":"Grain legumes, which are commonly referred to as pulses, are staple foods in many parts of the world, but are infrequently consumed in most economically developed countries where the obesity pandemic is prominent. However, even in low pulse consuming countries such as the United States, there are sub-groups of individuals who consume large amounts of pulses. Systematic reviews of population studies indicate that pulse consumers have a lower risk for developing obesity. To determine whether these population-based findings could be modeled in preclinical systems in which such findings can be deconstructed, we used rat and mouse models of dietary induced obesity and reported that lipid accumulation was inhibited. In this study, we examined the relationship between inhibition of fat accumulation and changes in the gut associated microbiome in male C57BL/6 mice fed either a high fat diet with casein as the protein source or that diet formulation in which one of four pulses (chickpea, common bean, dry pea, or lentil) was substituted to provide 70% dietary protein with the remainder provided by casein. The seeds of each pulse were soaked, cooked, and then freeze-dried and milled; the resulting powder was used for diet formulation. Mice were ad libitum fed over the 17-week duration of the feeding trial. Cecal content was obtained at necropsy and immediately snap frozen in liquid nitrogen. Extracted genomic DNA was processed for 16s rRNA sequencing on an Illumina system. Significant differences were observed between each pulse and the high fat control diet in microbial phylogenetic diversity (p < 0.001) and accumulation of lipid in adipose depots (p < 0.01). Differences among pulses were also observed in both metrics. Microbiome alpha and beta diversity metrics, differences in abundance for each detected taxon among treatment groups, and their relationships to changes in lipid accumulation in adipose storage depots are reported.","PeriodicalId":320592,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129977001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Metabolic-based therapies such as nutritional ketosis have been proven effective for seizure disorders and various acute and chronic neurological pathologies. In a healthy brain, glucose is the primary metabolic fuel for cells. However, neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), seizure disorders, and traumatic brain injury (TBI) are associated with impaired glucose transport and metabolism and with mitochondrial dysfunction leading to energy deficit. Therapeutic ketosis can be considered as a form of metabolic therapy by providing alternative energy substrates. In addition, ketosis leads to metabolic adaptations that improve brain metabolism, restore mitochondrial ATP production, decrease reactive oxygen species production, reduce inflammation, and increase the activity of neurotrophic factors. Moreover, the synaptic activity between neurons is also stabilized through the increase of Szent-Györgyi–Krebs cycle intermediates, antioxidant effects, increased GABA-to-glutamate ratio, and activation of ATP-sensitive potassium channels.
{"title":"Clinical Applications of Ketogenic Diet-Induced Ketosis in Neurodegenerative and Metabolism-Related Pathologies","authors":"R. Pilla","doi":"10.3390/iecn2020-06982","DOIUrl":"https://doi.org/10.3390/iecn2020-06982","url":null,"abstract":": Metabolic-based therapies such as nutritional ketosis have been proven effective for seizure disorders and various acute and chronic neurological pathologies. In a healthy brain, glucose is the primary metabolic fuel for cells. However, neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), seizure disorders, and traumatic brain injury (TBI) are associated with impaired glucose transport and metabolism and with mitochondrial dysfunction leading to energy deficit. Therapeutic ketosis can be considered as a form of metabolic therapy by providing alternative energy substrates. In addition, ketosis leads to metabolic adaptations that improve brain metabolism, restore mitochondrial ATP production, decrease reactive oxygen species production, reduce inflammation, and increase the activity of neurotrophic factors. Moreover, the synaptic activity between neurons is also stabilized through the increase of Szent-Györgyi–Krebs cycle intermediates, antioxidant effects, increased GABA-to-glutamate ratio, and activation of ATP-sensitive potassium channels.","PeriodicalId":320592,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126975606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Pérez-Cano, M. Castell, Patricia Ruiz-Iglesias, Raquel Gomez-Bris, M. Massot-Cladera, M. J. Rodríguez-Lagunas
Acute high-intensity exercise can impair the immune system, and lead to oxidative stress. Cocoa intake might help in protecting against oxidative damage and impaired immune functioning. The aim of this study was to establish the effect of cocoa and cocoa fibre on the oxidative status and the immunoglobulin (Ig) production of rats following a bout of acute exercise on a treadmill. The production of reactive oxygen species (ROS) by macrophages and the concentration of serum and mucosal Ig was assessed 16 h after the running session. Exercise increased ROS production and decreased the serum IgG concentration and the salivary gland IgM content. A cocoa fibre-enriched diet prevented the increased ROS production and the reduction in salivary IgM induced by exercise, although it decreased the IgA content in serum and the salivary glands. Overall, cocoa, by means of its fibre content, can partially prevent the alterations in ROS and Ig production induced by a single session of intensive running exercise.
{"title":"Cocoa and cocoa fibre intake modulate reactive oxygen species and immunoglobulin production in rats submitted to acute running exercise","authors":"F. Pérez-Cano, M. Castell, Patricia Ruiz-Iglesias, Raquel Gomez-Bris, M. Massot-Cladera, M. J. Rodríguez-Lagunas","doi":"10.3390/iecn2020-06990","DOIUrl":"https://doi.org/10.3390/iecn2020-06990","url":null,"abstract":"Acute high-intensity exercise can impair the immune system, and lead to oxidative stress. Cocoa intake might help in protecting against oxidative damage and impaired immune functioning. The aim of this study was to establish the effect of cocoa and cocoa fibre on the oxidative status and the immunoglobulin (Ig) production of rats following a bout of acute exercise on a treadmill. The production of reactive oxygen species (ROS) by macrophages and the concentration of serum and mucosal Ig was assessed 16 h after the running session. Exercise increased ROS production and decreased the serum IgG concentration and the salivary gland IgM content. A cocoa fibre-enriched diet prevented the increased ROS production and the reduction in salivary IgM induced by exercise, although it decreased the IgA content in serum and the salivary glands. Overall, cocoa, by means of its fibre content, can partially prevent the alterations in ROS and Ig production induced by a single session of intensive running exercise.","PeriodicalId":320592,"journal":{"name":"Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115236020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：