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Comparartive Analysis of Clarias gariepinus Fingerlings Reared in Pond and Cage Systems in Umuagwo, Imo State, Nigeria 尼日利亚伊莫州乌穆阿格沃池塘和笼养克拉利亚鱼鱼种的比较分析
Pub Date : 2023-03-14 DOI: 10.4314/jas.v37i1.10
C. Onwuka, L. E. Osuagwu, L. O. Igboachu
A study was conducted to compare performance of Clarias gariepinus reared in bamboo cage and concrete pond systems. The cage system measured 2.5m by 2.5m x 2m while the pond system measured 10m x 10m x 15m in length, width and depth respectively. The experiment was conducted between March and August 2019. The cage system composed of a predator barrier net constructed with galvanized angle rod and ½ copper wire mesh. Fish net was made up of ½ netting materials (210 D/9), nylon twine (210 D/9 and 210 D/8) and ropes (10mm PE and 4mm PE, as house in which the fish fingerlings were stocked. The stocked fish were held captive in the fish net cage while water flowed through it. This was assigned Treatment 1 (T1) while the concrete system was assigned Treatment 2 (T2). Each of the treatments was stocked with 1,500 Clarias fingerlings of 10g average weight and managed simultaneously for a period of 184 days. Data were analysed using analysis of variance. Results showed 83.3% and 96.6% survival rates in cage and pond systems respectively. Average weight at harvest per fish ranged between 332.6g and 415.7g for cage system and 250.6g and 350.8g for pond system. The survival rate in cage culture (83.3%) was low due to high water current, as cage was floated, hence fingerlings could not maximize feed intake at the initial stage. Results of weight frequency showed varying degrees of importance. For the cage culture the percentage weights varied between 12% in the group belonging to 251 – 300g in size having 150 number of fish to 21.6% in the group of 401 – 451g size with 270 fish specimen while in pond culture it varied between 3.45% in 401 – 451g size with 50 fish to 41.38% in 201 – 250g size having 600 fish samples. Inference from these results indicated that cage system had bigger fish at harvest than the pond system. From the results, the cage fish culture is more profitable in terms of weight and biomass. However, adequate care should be taken to ensure maximum utilization of feed in cage culture system. 
对竹笼和混凝土池两种饲养方式下的加里宾Clarias的生产性能进行了比较研究。笼子系统的尺寸为2.5m × 2.5m × 2m,池塘系统的长度、宽度和深度分别为10m × 10m × 15m。该实验于2019年3月至8月进行。笼子系统由一个由镀锌角杆和半铜丝网构成的捕食者屏障网组成。鱼网由半网材料(210 D/9)、尼龙绳(210 D/9和210 D/8)和绳索(10毫米聚乙烯和4毫米聚乙烯)组成,作为放养鱼种的小屋。放养的鱼被关在鱼网笼子里,水从里面流过。这被指定为治疗1 (T1),而混凝土系统被指定为治疗2 (T2)。每个处理放养平均体重为10g的克拉丽鱼鱼种1500尾,同时饲养184 d。采用方差分析对数据进行分析。结果网箱和池塘的成活率分别为83.3%和96.6%。收获时每条鱼的平均重量在网箱系统的332.6g和415.7g之间,池塘系统的250.6g和350.8g之间。网箱养殖时,由于网箱是浮动的,水流大,鱼种在初期不能最大限度地采食量,成活率较低(83.3%)。权重频率的结果显示出不同程度的重要性。网箱养殖的百分比重量在251 - 300g组的12%(150条鱼)和401 - 451g组的21.6%(270条鱼)之间变化,而池塘养殖的百分比重量在401 - 451g组的3.45%(50条鱼)和201 - 250g组的41.38%(600条鱼)之间变化。从这些结果推断,网箱系统在收获时比池塘系统有更大的鱼。从结果来看,网箱养鱼在重量和生物量方面更有利可图。然而,在网箱养殖系统中,应充分注意确保饲料的最大利用率。
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引用次数: 0
Preliminary study on the utilization of aqueous bark extracts of Khaya ivorensis in diets of Clarias gariepinus fingerlings 卡依树树皮水提物在加里皮Clarias gariepinus鱼种饲料中的应用初步研究
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.13
A. Alimi, G. Igili, O.K. Adeboyejo, N. Ikenweiwe
Mortality of fish seeds is recognised as one of the major factors responsible for serious economic losses in fish culture business. A preliminary investigation on the effects of aqueous extracts of dried African mahogany, Khaya ivorensis bark on the growth, survival, haematology and histology of Clarias gariepinus fingerlings was conducted. A total of 135 C. gariepinus fingerlings (1.41±0.03 g/fish) were randomly allocated into three treatments, T1 (Control), T2 and T3, and fed with three different diets (40% CP) D1, D2 and D3 containing 0g/L, 25g/L and 50g/L aqueous extracts of K. ivorensis bark respectively. The experimental fish were fed ad-libitum for a period of 28 days in rectangular plastic containers (15x25x30cm3 ) and water exchange was done daily. Experimental data were analyzed for statistical differences in the growth, survival indices, haematology and histology of experimental fishes using analysis of variance (ANOVA). There was no significant difference (p>0.05) in mean weight gain of Control (2.91±0.68g) and T2 (2.20±0.20g) but T3 (1.79±0.39g) was significantly low. There was a significant difference in the survival (S) of experimental fish with the S (%) values of 73.67±13.50, 68.67±16.50 and 57.00±10.00 for T2, T3 and control respectively, no significant different in the feed conversion ratio (p>0.05) in the experimental groups. The haematological characteristics of all experimental fish were not significantly different, though the packed cell volume, PCV (%), haemoglobin count, HB (g/dl) and red blood cell, RBC (million/mm) were in the order T2˃T3˃Control having mean values of 22.71±4.5˃21.67±5.1˃19.67±1.53 (PCV); 22.67±4.51˃2.67±6.51˃19.67±1.53 (HB) and 2.09±0.47>1.99±0.57>1.73±0.13 (RBC) for T2, T3 and control respectively. The white blood cell, (No/mm) was highest in T1 (4.03±0.25) and least in T3 (3.67±1.35). Although there was no degeneration, the histological characteristics of liver of the experimental fish ranged from moderately diffused to fewer hepatocytes when compared to the normal liver, of the control. It is therefore concluded that Khaya ivorensis bark can be included in the diet of Clarias gariepinus fingerlings at 25g/L aqueous extract for good growth and high survival rates of fish under culture Keywords: Medicinal herbs, Extracts, African catfish, Fish culture
鱼类种子的死亡被认为是造成养鱼业严重经济损失的主要因素之一。本文初步研究了非洲红木树皮水提物对克拉丽亚鱼(Clarias gariepinus)鱼种生长、存活、血液学和组织学的影响。试验选取135尾(1.41±0.03 g/尾)加肋棘鱼鱼种,随机分为T1(对照)、T2和T3 3个处理,分别饲喂含0g/L、25g/L和50g/L树皮水提物的D1、D2和D3 3种不同饲粮(40% CP)。试验鱼在矩形塑料容器(15x25x30cm3)中自由饲喂,为期28天,每天换水。采用方差分析(ANOVA)对实验鱼的生长、存活指标、血液学和组织学进行统计学差异分析。对照组(2.91±0.68g)与T2组(2.20±0.20g)的平均增重无显著差异(p>0.05), T3组(1.79±0.39g)显著低于对照组(2.91±0.68g)。试验鱼的存活率(S)在T2、T3和对照组分别为73.67±13.50、68.67±16.50和57.00±10.00,差异有统计学意义(p>0.05),饲料系数在试验组间无显著差异(p>0.05)。所有实验鱼的血液学特征无显著差异,但堆积细胞体积、PCV(%)、血红蛋白计数、HB (g/dl)和红细胞、RBC(百万/mm)均为T2、T3、Control,平均值为22.71±4.5、21.67±5.1、19.67±1.53 (PCV);T2、T3和对照组HB分别为22.67±4.51、2.67±6.51、19.67±1.53、2.09±0.47>1.99±0.57>1.73±0.13。白细胞(No/mm) T1最高(4.03±0.25),T3最低(3.67±1.35)。虽然没有变性,但与对照组的正常肝脏相比,实验鱼肝脏的组织学特征从中度扩散到较少的肝细胞。综上所述,在饲料中添加25g/L水提物时,可提高鱼的生长和成活率。关键词:药材,提取物,非洲鲶鱼,鱼类养殖
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引用次数: 0
Microbiological properties of water and sediment of New Calabar River, Port Harcourt, Nigeria 尼日利亚哈科特港新卡拉巴尔河水和沉积物的微生物特性
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.19
B. B. Otene, K. Chukwu, F.S Pepple
Microbiological characteristics of water and sediment of New Calabar, River, Port Harcourt, Nigeria were investigated. Water and sediment samples were collected from three stations {Aluu, ARAC (Station 1; Choba Bridge (Station 2) and Rumuokparali (Station 3)} on a monthly basis between February and April, 2020 and analyzed using standard methods. The results showed that Total Heterotrophic Bacteria in water (THBw) ranged between 2.20 x 102 and 2.6x104 cfu/ml with the overall mean value of 7,313.33 + 1039.09 cfu/ml while that of sediment (THBs) ranged between 3.1 x 103 and 3.9 x 104 cfu/g with the overall mean value of 42,566.67 + 71199cfu /g. The THB counts were higher in sediment than in water with Station 2 significantly different from other stations. Total Coliform Bacteria (TCB) in water ranged between 1.1 x 103 cfu/ml and 2.9 x 103 cfu/ml with the mean value of 1988.89±532.55cfu/ml while that of sediment ranged between 1.3 x 103 cfu/g and 2.5 x 104 cfu/g with the mean value of 9422.22 ±9595.41cfu/g. TCB counts in water showed no significant difference across the stations at p<0.05 but Station 2 statistically differed from Stations 1 and 3 in the sediment. Morphological and biochemical tests showed that 8 bacteria species were isolated from sediment and and 9 specimens were recovered from water. Some isolated specimens were: Bacillus spp, Staphylococcus. sp, Bacillus cereus and Bacillus subtilis while Escherichia coli was the only bacterium found in water but absent in sediment. Two species: Staphylococcus spp and Bacillus subtilis dominated the study area. Fungal counts in water ranged between 3.0 x102 and 2.6 x 103 cfu/ml with the overall mean value of 1911.11±67.51cfu/ml while that of sediment ranged between 1.7 x101 and 3x102 with the overall mean value of 173.00±78.52 cfu/g. Fungi counts showed significant difference (p < 0.05) across the stations in water and sediment. Macroscopic and microscopic characterization of fungi showed that 6 fungi species were recovered in water and sediment. These included Aspergillus niger, Rhizopus oligospora and Pennicillium species. The species Fusarium solani and Aspergillus flavor were found in Station 1 but absent in Stations 2 and 3. It could be observed that microbial counts in both water and sediment exceeded the 1.0x102 cfu/ml and 0 cfu/ml recommended levels by WHO and EPA respectively. Thus, the study area may be under threat and the water may be unsafe for human consumption. Keywords: Microbiological Properties, Sediment, Water, New Calabar River, Port Harcourt
研究了尼日利亚哈科特港新卡拉巴尔河水体和沉积物的微生物特征。从三个站点收集了水和沉积物样本{Aluu, ARAC(站1;选取2020年2 - 4月间的长叶桥(2号站)和鲁木克帕拉利(3号站),采用标准方法进行分析。结果表明:水体总异养菌(THBw)数量在2.20 × 102 ~ 2.6 × 104 cfu/ml之间,总体平均值为7313.33 + 1039.09 cfu/ml;沉积物总异养菌(THBs)数量在3.1 × 103 ~ 3.9 × 104 cfu/g之间,总体平均值为42566.67 + 71199cfu /g。沉积物中THB计数高于水中,2号站位与其他站位差异显著。水体总大肠菌群(Total Coliform Bacteria, TCB)在1.1 × 103 cfu/ml ~ 2.9 × 103 cfu/ml之间,平均值为1988.89±532.55cfu/ml;沉积物总大肠菌群(Total Coliform Bacteria, TCB)在1.3 × 103 cfu/g ~ 2.5 × 104 cfu/g之间,平均值为9422.22±9595.41 1cfu/g。水体中TCB计数在各站点间差异不显著(p<0.05),而沉积物中2站与1站和3站差异有统计学意义。形态学和生化试验表明,从沉积物中分离到8种细菌,从水中回收到9种标本。部分分离标本有:芽孢杆菌、葡萄球菌。大肠杆菌是水中唯一的细菌,沉积物中没有大肠杆菌。研究区以葡萄球菌和枯草芽孢杆菌两种为主。水中真菌计数范围为3.0 × 102 ~ 2.6 × 103 cfu/ml,总体平均值为1911.11±67.51cfu/ml;沉积物真菌计数范围为1.7 × 101 ~ 3 × 102,总体平均值为173.00±78.52 cfu/g。水体和沉积物中真菌数量在各站点间差异显著(p < 0.05)。真菌的宏观和微观表征表明,在水体和沉积物中发现了6种真菌。其中包括黑曲霉、少孢子根霉和青霉。1号站有枯萎菌和风味曲霉,2号站和3号站没有。水和沉积物中的微生物数量分别超过了WHO和EPA建议的1.0x102 cfu/ml和0 cfu/ml。因此,研究区域可能受到威胁,水可能对人类消费不安全。关键词:微生物特性,沉积物,水,新卡拉巴尔河,哈科特港
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引用次数: 0
Cockroach (Periplaneta Americana) as fishmeal replacer in diets of African catfish (Clarias gariepinus) Juveniles 美洲大蠊在非洲鲶鱼幼鱼饲料中的替代鱼粉作用
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.11
S. O. Sule, A. Sotolu, S. Yakubu, R. Mohammed
The utilization of insects as feed has witnessed dramatic inclusion in livestock and aquaculture diets. The farming of cockroach (Periplaneta americana) is gaining acceptance among industries. However, research on its use in livestock and fish nutrition is scanty. This study aimed at investigating the growth of Clarias gariepinus juveniles fed cockroach insect meal (CIM) as fishmeal (FM) replacer. One hundred and fifty Clarias gariepinus juveniles (6.29 ± 0.06 g) were stocked at 10 fish/tank/replicate and fed 40% crude protein diets formulated thus: Diet 1 (0% CIM; control), Diet 2 (25% CIM), Diet 3 (50% CIM), Diet 4 (75% CIM) and Diet 5 (100% CIM). Fish were fed at 5% of their body weight twice daily for 56-day experimental period. The results showed that final weight, mean weight gain, specific growth rate, protein efficiency and food conversion ratios of fish fed 25 and 50% insect meal-based diets were not significantly different (p>0.05) from those treated with the control diet. Survival rates were not significantly different in all dietary treatments. Quadratic regression analysis revealed that insect meal can be used at up to 58% inclusion level in diets of C. gariepinus juveniles for optimal growth. Keywords: Amino acid, Blattodea, fish-nutrition, growth performance, C. gariepinus
利用昆虫作为饲料已被大幅度地纳入牲畜和水产养殖饲料中。蟑螂(美洲大蠊)的养殖正在各行业中得到认可。然而,关于其在牲畜和鱼类营养中的应用的研究很少。本试验旨在研究以蟑螂昆虫粉(CIM)代替鱼粉(FM)对加里宾Clarias gariepinus幼鱼的生长情况。试验以10尾/缸/重复的方式放养150尾(6.29±0.06 g)甲尾Clarias gariepinus幼鱼,投喂40%粗蛋白质饲料,配制如下:对照组)、日粮2 (25% CIM)、日粮3 (50% CIM)、日粮4 (75% CIM)和日粮5 (100% CIM)。试验期56 d,按体重的5%饲喂,每天2次。结果表明:饲喂25%和50%昆虫粉饲料的鱼的末重、平均增重、特定生长率、蛋白质效率和食物转化率与对照饲料无显著差异(p>0.05)。不同饮食处理的存活率无显著差异。二次回归分析表明,在饲料中添加58%的昆虫粉可达到最佳生长效果。关键词:氨基酸,虾蛄,鱼类营养,生长性能,鱼蛄
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引用次数: 1
Survival rate, growth and condition index of tank-raised Clarias gariepinus under different salinities 不同盐度条件下池养金鱼的存活率、生长状况及状态指数
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.20
E. Ayim, S. Ameh, C. B. Ndome, E. Okon
The study evaluated varying levels of salinity on survival, growth and condition index of tank raised Clarias gariepinus for ten weeks. A total of 120 healthy C. gariepinus fingerlings having a bulk weight and average length of 131.73 ± 0.85 g and 5.06 ± 0.24 cm respectively were procured from the hatchery complex of University of Calabar fish farm and used for the study. Sea water was collected from Ibeno Beach and diluted with borehole water to obtain various salinity concentrations of ≤1, ≤5, ≤10 and ≤15 ppt. This represented four treatments(Tanks A to D) and each treatment had three replicates. Evaluation of growth indices and culture water parameters followed standard procedure. Results revealed significant (p < 0.05) differences with regards to food intake in the treatment Tanks A (≤1ppt), B (≤5ppt), C (≤10ppt) and D (≤15ppt). Food conversion efficiency (FCE), ratio (FCR) and protein efficiency ratio (PER) were significantly different (p < 0.05). Body weight gain were observed to be higher in Tank A with a value of 75.83± 0.07g/kg and a total length of 11.13 ±0.38cm and the lowest weight gain was recorded in Tank D with a value of 43.56 ± 0.76g/kg and a mean total length of 6.67± 0.43cm. There were significant differences (p < 0.05) in the final condition factor (K) of the fish. Hence, it could be established that C. gariepinus fingerlings can survive and be reared in 5ppt saline water without any adverse effect on growth. At salinity level above 5ppt, fish growth rate may be slow. Keywords: Survival rate, Growth, Clarias gariepinus, Salinity, Physicochemical parameters
本研究评价了不同盐度水平对10周池养Clarias gariepinus的生存、生长和状态指数的影响。从卡拉巴大学鱼场孵化场获得体重为131.73±0.85 g、平均体长为5.06±0.24 cm的健康加里滨梭子鱼鱼种120尾,用于研究。从Ibeno海滩收集海水,用钻孔水稀释,得到≤1、≤5、≤10和≤15 ppt的不同盐度浓度。这代表4个处理(池A至D),每个处理有3个重复。按标准程序评价生长指标和培养水参数。结果显示,A(≤1ppt)、B(≤5ppt)、C(≤10ppt)和D(≤15ppt)处理池的摄食量差异有统计学意义(p < 0.05)。饲料转化率(FCE)、饲料比率(FCR)和蛋白质效率(PER)差异显著(p < 0.05)。A池体增重最高,为75.83±0.07g/kg,体长为11.13±0.38cm; D池体增重最低,为43.56±0.76g/kg,体长为6.67±0.43cm。鱼的最终状态因子(K)差异显著(p < 0.05)。由此可以确定,在5ppt的盐水中饲养,加里滨沙棘鱼鱼种可以存活,对其生长无不良影响。当盐度高于5ppt时,鱼类的生长速度可能较慢。关键词:存活率,生长,克拉里巴斯,盐度,理化参数
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引用次数: 0
Socio-economic factors affecting fisherfolks in Otuocha and Ebenebe fishing communities in Anambra state, Nigeria 影响尼日利亚阿南布拉州Otuocha和Ebenebe渔业社区渔民的社会经济因素
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.15
C. C. Ikechukwu, M. Chukwurah, C. Nwankwo, H. F. Ogbonnaya, I. Edeh
Fishing is an important source of income contributing to the economy of fishing communities living around Ezu and Omamabala Rivers in Ebenebe and Otuocha areas in Anambra State, Nigeria. A total of 120 structured questionnaires were distributed to obtain the socio-economic information from the respondents. Descriptive statistic was used for the data analysis. Results from the study revealed significant differences (p < 0.05) between Ebenebe and Otuocha fishing communities, in terms of level of education attained, with no significant difference (p > 0.05) in the average monthly income earned. The study also showed that 71.7% of the respondents were males which engaged in fish farming while 28.3% were females involving in fish processing and trading, both of which were mostly within the age bracket of 30-39 years. The highest household size (35.9%) comprised of 6 members while 92.3% of the respondents had one form of education or the other. Most of the respondents (69.6%) took fishing as their major occupation while 30.4% also involved in other activities in addition to fishing operations. A 5-point likert scale was employed to record challenges of the respondents, and could be concluded that the socio-economic conditions of the fisherfolks in the study areas were not highly excellent due to lack of basic social amenities and modern fishing facilities amongst other factors. Keywords: Fisher folks, Social-Economic factors, Ebenebe, Otuocha, Anambra
渔业是尼日利亚阿南布拉州埃比贝和奥图查地区埃祖河和奥马巴拉河附近渔业社区的重要收入来源,为当地经济做出了贡献。共发放了120份结构化问卷,以获取受访者的社会经济信息。采用描述性统计进行数据分析。研究结果显示,Ebenebe和Otuocha渔业社区在受教育程度方面存在显著差异(p < 0.05),而在平均月收入方面无显著差异(p < 0.05)。研究还表明,71.7%的受访者是从事养鱼的男性,28.3%的受访者是从事鱼品加工和贸易的女性,这两个群体的年龄大多在30-39岁之间。家庭人数最多的是6人(35.9%),92.3%的受访者接受过某种形式的教育。大部分回答者(69.6%)以捕鱼为主要职业,而30.4%的回答者除从事捕鱼作业外,亦从事其他活动。采用5点李克特量表记录受访者的挑战,可以得出结论,由于缺乏基本的社会设施和现代捕鱼设施等因素,研究地区渔民的社会经济条件不是很好。关键词:渔民,社会经济因素,埃比贝,奥图查,阿南布拉
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引用次数: 1
Prevalence of gastrointestinal parasites in tilapia (Oreochromis niloticus) in Port Harcourt, Rivers state, Nigeria 尼日利亚河流州哈科特港罗非鱼(Oreochromis niloticus)胃肠道寄生虫流行情况
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.16
O. A. Bubu-Davies, O. Abraham-Akosubo, A. Ilesanmi, G. Umah
The study aimed to investigate the prevalence of gastrointestinal parasites in Oreochromis niloticus sold in major markets in Port Harcourt, Rivers State, Nigeria. A total of 120 specimens of different size groups were randomly sampled from three (3) markets in Port Harcourt and examined for parasites. The overall frequency and percentage prevalence recorded was 80 (66.67%) with the highest parasitic infection obtained in Rumuokoro market samples. Out of the total fish sampled, the prevalence of gastrointestinal parasite in O. niloticus sampled from Oil Mill market was 26 (21.6%), Rumuokoro market 30 (25%) and Rumuola market 24 (20%). The overall prevalence showed significant difference (p = 0.005) in all the markets. However, percentage prevalence between sex showed no significant difference (p = 2.46), with male fishes known to have a higher prevalence of infection. The recovered parasites were protozoans>nematodes>cestodes>trematodes in order of prevalence as 40 (33.3%) > 30 (25%) >16 (13.33%) >12 (10%) respectively. Thus, protozoans showed the highest prevalence in O. niloticus. In conclusion, fish specimens were found to be infected with various gastrointestinal parasites, this may lead to reduction in productivity. Keywords: Parasites, digestive system, prevalence, Oreochromis niloticus, Port Harcourt
本研究旨在调查尼日利亚河流州哈科特港主要市场上销售的尼罗ticus消化道寄生虫的流行情况。从哈科特港的三个市场随机抽取了120个不同大小群体的样本,并检查了寄生虫。总频次和流行率为80%(66.67%),其中鲁木科罗市场样本寄生虫感染率最高。其中,油磨市场、鲁穆可罗市场和鲁穆拉市场分别有26条(21.6%)、30条(25%)和24条(20%)niloticus胃肠道寄生虫。各市场总体患病率差异有统计学意义(p = 0.005)。然而,性别之间的感染率没有显着差异(p = 2.46),已知雄性鱼具有更高的感染率。检出的寄生虫依次为原生动物b>、线虫b>、寄生虫b>、吸虫b>,患病率依次为40(33.3%)、b>、30(25%)、b>、16(13.33%)、b>、12(10%)。因此,原生动物在niloticus中患病率最高。总之,鱼类标本被发现感染了各种胃肠道寄生虫,这可能导致生产力下降。关键词:寄生虫,消化系统,流行,尼罗褐口鱼,哈考特港
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引用次数: 0
Spawning performance and survival of Clarias gariepinus fry bred under different water sources in Abakaliki Metropolis 阿巴卡利基市不同水源条件下养殖的鱼苗产卵性能及成活率
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.18
J. Awoke, E. E. Oti, C. B. Okoro
The study was conducted to compare effects of three different water sources on hatchability and survival of Clarias gariepinus fry. The water sources used were: rain (RNW), borehole (BHW) and well (WLW). The experiment involved artificial induced breeding of C. gariepinus and rearing the resultant larvae/hatchlings for 14 days to assess reproductive performance. Hatchability was estimated 24 hours after incubation, while larval survival was determined at 14 days’ post hatching. Data resulting from the experiment were subjected to one-way analysis of variance (ANOVA). Percentage hatchability in all water sources were relatively high with the highest mean value of 93.1±1.60% obtained in RNW experiment. Larval survival after 14 days’ post hatching was above average with the highest mean value of 63.7±2.41% recorded in BHW while RNW recorded the lowest mean value of 44.2±0.55%. The results of this study has demonstrated that borehole water may be a suitable water source for induced artificial spawning of the African catfish and subsequent larval rearing. Keywords: Brood Stock, Hypophysation, Propagation, Water quality, Milt, Hatching
本研究比较了3种不同水源对Clarias gariepinus鱼苗孵化率和存活率的影响。利用的水源为:雨水(RNW)、钻孔(BHW)和井(WLW)。本试验采用人工诱导繁殖的方法,饲养14 d,观察其繁殖性能。孵化后24小时估计孵化率,孵化后14天测定幼虫存活率。实验数据采用单因素方差分析(ANOVA)。各水源的孵化率均较高,RNW试验的孵化率平均值最高,为93.1±1.60%。孵化后14 d幼虫存活率高于平均水平,BHW的平均值最高,为63.7±2.41%,RNW的平均值最低,为44.2±0.55%。本研究结果表明,钻孔水可能是非洲鲶鱼人工诱导产卵和随后的幼虫饲养的合适水源。关键词:育雏,产卵,繁殖,水质,卵,孵化
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引用次数: 0
Fatty acid composition of fish feeds vended in Lagos state, Nigeria 尼日利亚拉各斯州出售的鱼饲料的脂肪酸组成
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.14
M. Ezekiel, M. M. Salaudeen, O. Esan, I. A. Uzoalu, C. Igwe
Fatty acid compositions of the lipids extracted from various fish feeds (Eco-float, Blue-crown, Uncle - frances, Triton, Stretting, Crown, Jik, Shaldac, Solace, Coppens, Vital, Local sinking, Local feed, Aqualis and Local floating) vended in Lagos State were determined. These feeds were designated as Feeds A to O respectively. Feed samples were procured from major fish feed markets and fish farms within Lagos metropolis and transported to the laboratory for analysis. Methylation was carried out after oil extraction to convert the fats to their methyl esters and the fatty acids methyl esters (FAME) were separated by gas chromatography. Fatty acids were analysed using a HP Agilent 5890 system gas chromatograph equipped with SP-2330 and a flame ionization detector (FID). Results revealed that the highest values for polyunsaturated fatty acid (PUFA), saturated fatty acid (SFA) and ∑n-3 unsaturated fatty acid (HUFA) were 58.26% in feed F, 38.7% in feed M and 3.59% in feed N respectively while the lowest values for PUFA (19.76%) and SFA (15.53%) were recorded in feeds M and J respectively. The results also showed that, though feed samples were high in monounsaturated fatty acid (MUFA) (55- 22%) and PUFA (58-19%), they were low in n-3 HUFA {eicosapentaenoic acids (EPA, 20:5n-3) and docosahexaenoic acids (DHA, 22:6n-3)} with the values of 0.3-3%. The shortfall in EPA and DHA of these feeds may imply that fish fed these feeds may be nutritionally deficient in these fatty acid compositions. Thus, further manipulation may be needed to improve the value of these feeds in terms of EPA and DHA. Keywords: Fish feeds, n-3 HUFA, n-6 HUFA, Nutritional quality, aquaculture
测定了从拉各斯州出售的各种鱼类饲料(Eco-float、Blue-crown、Uncle - frances、Triton、Stretting、Crown、Jik、Shaldac、Solace、Coppens、Vital、Local下沉、Local饲料、Aqualis和Local浮动)中提取的脂质脂肪酸组成。这些feed分别被指定为feed A到O。饲料样本是从拉各斯大都会内的主要鱼饲料市场和养鱼场采购的,并运往实验室进行分析。提取油脂后进行甲基化,将油脂转化为甲酯,并采用气相色谱法分离脂肪酸甲酯。脂肪酸分析使用HP Agilent 5890系统气相色谱仪,配备SP-2330和火焰电离检测器(FID)。结果表明,饲料F、饲料M和饲料N的多不饱和脂肪酸(PUFA)、饱和脂肪酸(SFA)和∑N -3不饱和脂肪酸(HUFA)含量最高,分别为58.26%、38.7%和3.59%,饲料M和饲料J的PUFA和SFA含量最低,分别为19.76%和15.53%。结果还表明,饲料样品中单不饱和脂肪酸(MUFA)和多聚脂肪酸(PUFA)含量较高(55 ~ 22%),但n-3 HUFA{二十碳五烯酸(EPA, 20:5n-3)和二十二碳六烯酸(DHA, 22:6n-3)}含量较低(0.3 ~ 3%)。这些饲料中EPA和DHA的不足可能意味着饲喂这些饲料的鱼可能缺乏这些脂肪酸组成的营养。因此,可能需要进一步的操作来提高这些饲料在EPA和DHA方面的价值。关键词:鱼饲料,n-3 HUFA, n-6 HUFA,营养品质,水产养殖
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引用次数: 0
Recovery rate, haematological parameters and growth responses of Heterobranchus longifilis exposed to zinc oxide nanoparticles 氧化锌纳米颗粒对长尾异枝的回收率、血液学参数和生长响应
Pub Date : 2022-06-13 DOI: 10.4314/jas.v36i2.17
S. Abdulkareem, O. D. Owolabi
The present study investigated the toxicity effects of zinc oxide nano-particles (ZnO-NPs) on haematological parameters and growth responses of Heterobranchus longifilis. Catfish juveniles were exposed to sub-lethal concentrations (0.00, 6.00, 8.00, 10.00 and 12.00 mg/l) of zinc oxide nano-particles in static renewal bioassays for 60 days. The ZnO-NPs-exposed fish were then subjected to depuration phase for 30 days to monitor the recovery ability of the fish. After each experimental stage, fish were sacrificed and blood was collected for haematological variables such as: erythrocytes {Red blood cells (RBC), packed cell volume (PCV) and haemoglobin (HB)} and leucocytes {White blood cells (WBC), platelets (PLT), lymphocyte (LYMPH) and neutrophil (NEUT)} using standard procedure. Results revealed reduction (p < 0.05) in the values of RBC counts (0.70 x 106 /µl) in 10.00 and 12.00mg/l ZnO-NPs treated fish when compared to control group (3.70 x 106 /µl). A reverse trend was observed with significantly (p < 0.05) high WBC counts (220.70 x 103 /µl) in group treated with the highest concentration (12.00mg/l) of ZnO-NPs after 60 days of exposure. The recovery experiment followed a different trend with a gradual decrease in RBC values from 2.2 x 106 /µl in 6.00mg/l to 2.00 x 106 /µl in 12.00mg/l while WBC counts increased from 182.00 x 103 /µl in 6.00 mg/l to 194.30 x 103 /µl in 12.00mg/l. The highest percentage weight gain (58%) was recorded in the control group with the lowest (26%) in the ZnO-NPs-exposed group. However, fish growth increased as the depuration period increased. The results of this findings indicated that ZnO-NPs may have some adverse effects on the physiology of H. longifilis, however, the fish showed some ability to recover from the effects over time. Keywords: Depuration, Haematology, Heterobranchus longifilis, ZnO-NPs
本文研究了氧化锌纳米颗粒(ZnO-NPs)对长鳃异枝血液学参数和生长反应的毒性影响。以亚致死浓度(0.00、6.00、8.00、10.00和12.00 mg/l)的氧化锌纳米颗粒对鲶鱼幼鱼进行静态更新生物试验60 d。然后对接触zno - nps的鱼进行30天的净化阶段,以监测鱼的恢复能力。每个实验阶段结束后,处死鱼,采集血液,按标准程序测定血液学指标,如红细胞{红细胞(RBC)、堆积细胞体积(PCV)和血红蛋白(HB)}和白细胞{白细胞(WBC)、血小板(PLT)、淋巴细胞(LYMPH)和中性粒细胞(NEUT)}。结果显示,10.00和12.00mg/l ZnO-NPs处理鱼的RBC计数值(0.70 × 106 /µl)比对照组(3.70 × 106 /µl)降低(p < 0.05)。以最高浓度(12.00mg/l) ZnO-NPs处理60 d后,WBC计数明显升高(220.70 × 103 /µl) (p < 0.05)。恢复实验遵循不同的趋势,RBC值从6.00mg/l的2.2 × 106 /µl逐渐下降到12.00mg/l的2.00 × 106 /µl,而白细胞计数从6.00mg/l的182.00 × 103 /µl增加到12.00mg/l的194.30 × 103 /µl。对照组体重增加百分比最高(58%),zno - nps暴露组体重增加百分比最低(26%)。然而,随着净化期的延长,鱼类的生长增加。研究结果表明,ZnO-NPs可能对长尾鱼的生理有一定的不利影响,但随着时间的推移,长尾鱼表现出一定的恢复能力。关键词:净化,血液学,长鳃异枝,ZnO-NPs
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引用次数: 0
期刊
Turkish Journal of Aquatic Sciences
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