Pub Date : 2023-01-01DOI: 10.5376/mpb.2023.14.0016
Yongmeng Gao, Jun Xie, Xiaoduo Lu, Haiyan Li
{"title":"ZmIQD27 Interacts with ZmCaMs and ZmKLCRs in Maize","authors":"Yongmeng Gao, Jun Xie, Xiaoduo Lu, Haiyan Li","doi":"10.5376/mpb.2023.14.0016","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0016","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"124 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80204398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5376/mpb.2023.14.0002
Fuan Niu, Huangwei Chu, Bin Sun, Jihua Zhou, An-peng Zhang, Yiwen Huang, Yao Li, Yao Yao, Can Cheng, Liming Cao
{"title":"Molecular Marker Assisted Breeding of a New Japonica Hybrid Rice","authors":"Fuan Niu, Huangwei Chu, Bin Sun, Jihua Zhou, An-peng Zhang, Yiwen Huang, Yao Li, Yao Yao, Can Cheng, Liming Cao","doi":"10.5376/mpb.2023.14.0002","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0002","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"27 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72602673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese water chestnut ( Eleocharis dulcis ) is an important characteristic vegetable that grows in shallow waters. In order to develop simple sequence repeat (SSR) molecular markers for genetics research on Eleocharis dulcis , simplified genome sequencing by restriction-site associated DNA sequencing (RAD-seq), SSR search and primer design were performed. A total of 5039 SSR loci were detected, from which 4 137 pairs of primer were designed. Among them, the proportion of tri-nucleotides motifs was the highest (1 894), accounting for 45.89% of the total loci; followed by di-nucleotide with 1 406, accounting for 34.07%.. A total of 100 pairs of randomly selected primers were verified. 93% of the primers were successfully amplified. To assess the polymorphism of these primers, two varieties of Chinese water chestnuts were used to amplify the SSR marker using 83 pairs of primers. The 83 pairs of primers generated a total of 232 fragments. A total of 128 fragments were polymorphic. 60 pairs of primers out of 83 pairs generated at least one polymorphic band, and the ratio of polymorphic primers was 72.28%. These SSR markers developed from RAD tags were proved to be effective, which will provide a useful tool for genetic diversity research and molecular breeding in Chinese water chestnuts.
{"title":"Development of Polymorphic SSR Markers in Chinese Water Chestnut Based on RAD-seq","authors":"Lianfang He, Weiqing Dong, Zuyang Qiu, Huiping Jiang, Lili Liu, Qi Chen, Shiyu Huang","doi":"10.5376/mpb.2023.14.0018","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0018","url":null,"abstract":"Chinese water chestnut ( Eleocharis dulcis ) is an important characteristic vegetable that grows in shallow waters. In order to develop simple sequence repeat (SSR) molecular markers for genetics research on Eleocharis dulcis , simplified genome sequencing by restriction-site associated DNA sequencing (RAD-seq), SSR search and primer design were performed. A total of 5039 SSR loci were detected, from which 4 137 pairs of primer were designed. Among them, the proportion of tri-nucleotides motifs was the highest (1 894), accounting for 45.89% of the total loci; followed by di-nucleotide with 1 406, accounting for 34.07%.. A total of 100 pairs of randomly selected primers were verified. 93% of the primers were successfully amplified. To assess the polymorphism of these primers, two varieties of Chinese water chestnuts were used to amplify the SSR marker using 83 pairs of primers. The 83 pairs of primers generated a total of 232 fragments. A total of 128 fragments were polymorphic. 60 pairs of primers out of 83 pairs generated at least one polymorphic band, and the ratio of polymorphic primers was 72.28%. These SSR markers developed from RAD tags were proved to be effective, which will provide a useful tool for genetic diversity research and molecular breeding in Chinese water chestnuts.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135651976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5376/mpb.2023.14.0013
F. Peng, Pinghua Fan, Kaikai Zhu, Pengpeng Tan, Yongrong Ling
{"title":"Identification of Gene Family in Pecan (Carya illinoinensis) and Its Expression in Graft Healing","authors":"F. Peng, Pinghua Fan, Kaikai Zhu, Pengpeng Tan, Yongrong Ling","doi":"10.5376/mpb.2023.14.0013","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0013","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73495341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5376/mpb.2023.14.0011
Min Wang, Jinxiu Chen, Xiang Tai, Xiao-Feng Zhu, Cancan Zhu, Tianyue Bo
WRKY gene family encodes a large transcription factor family that play critical roles in various physiological processes. However, a systematic analysis of the WRKY transcription factor family has not been reported in pecan ( Carya illinoinensis ). In this study, a total of 89 putative pecan WRKY genes ( CiWRKYs ), named CiWRKY1-89 , were identified from the whole genome of pecan. Most of WRKY domain sequences in CiWRKY proteins were “WRKYGQK”, but there were “WRKYGKK” in CiWRKY19, CiWRKY27 and CiWRKY88, and “WKKYGQK” in CiWRKY85. All CiWRKYs were unevenly distributed on 16 chromosomes, the largest number of genes on chromosome 3. Based on phylogenetic analysis, the 89 putative CiWRKYs could be classified into three major groups. The CiWRKY genes shared similar exon-intron distribution, and conserved motifs within the same subgroups. Expression profiles indicated that CiWRKY16 , CiWRKY30 , CiWRKY42 , CiWRKY62 , and CiWRKY69 genes were involved in flower differentiation and development, and majority of CiWRKYs genes were differentially expressed during embryo development. The present study provides reference for further comparative genomics and functional studies of this important class of transcriptional regulators in pecan.
{"title":"Identification and Expression Profiles of the WRKY Gene Family in Pecan (Carya illinoinensis)","authors":"Min Wang, Jinxiu Chen, Xiang Tai, Xiao-Feng Zhu, Cancan Zhu, Tianyue Bo","doi":"10.5376/mpb.2023.14.0011","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0011","url":null,"abstract":"WRKY gene family encodes a large transcription factor family that play critical roles in various physiological processes. However, a systematic analysis of the WRKY transcription factor family has not been reported in pecan ( Carya illinoinensis ). In this study, a total of 89 putative pecan WRKY genes ( CiWRKYs ), named CiWRKY1-89 , were identified from the whole genome of pecan. Most of WRKY domain sequences in CiWRKY proteins were “WRKYGQK”, but there were “WRKYGKK” in CiWRKY19, CiWRKY27 and CiWRKY88, and “WKKYGQK” in CiWRKY85. All CiWRKYs were unevenly distributed on 16 chromosomes, the largest number of genes on chromosome 3. Based on phylogenetic analysis, the 89 putative CiWRKYs could be classified into three major groups. The CiWRKY genes shared similar exon-intron distribution, and conserved motifs within the same subgroups. Expression profiles indicated that CiWRKY16 , CiWRKY30 , CiWRKY42 , CiWRKY62 , and CiWRKY69 genes were involved in flower differentiation and development, and majority of CiWRKYs genes were differentially expressed during embryo development. The present study provides reference for further comparative genomics and functional studies of this important class of transcriptional regulators in pecan.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86066620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Nutritional and Functional Components and Total Antioxidant Capacity of Different Momordica charantia Varieties","authors":"Hongmei Zhang, Haijun Jin, Jizhu Yu, Jiawei Cui, Lizhong He, Xiaotao Ding","doi":"10.5376/mpb.2023.14.0010","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0010","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81319221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of High Temperature and Waterlogging Stress on Cellulase Activity of Non-heading Chinese Cabbage","authors":"Hongfang Zhu, Lu Gao, Xiaoyan He, Xiaofeng Li, Dandan Xi, Yuying Zhu","doi":"10.5376/mpb.2023.14.0008","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0008","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85308916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5376/mpb.2023.14.0001
Sisi Liu, Zhongquan Qiao, Huijie Zeng, Yongxin Li, Neng Cai, Xin-min Liu, Xiaoming Wang
{"title":"Analysis on SSR Loci in Transcriptome and Development of EST-SSR Molecular Markers in Lonicera macranthoides","authors":"Sisi Liu, Zhongquan Qiao, Huijie Zeng, Yongxin Li, Neng Cai, Xin-min Liu, Xiaoming Wang","doi":"10.5376/mpb.2023.14.0001","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0001","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88773205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Sodium Nitroprusside on the Growth and Enzymes Related to Nitrogen Metabolism of Melon Seedlings under Low Temperature Stress","authors":"Yongping Zhang, Q. Diao, Yanyan Cao, Mengya Shi, Hongmei Zhang, Haijun Jin, Shouyang Jiang, Haibin Shen","doi":"10.5376/mpb.2023.14.0009","DOIUrl":"https://doi.org/10.5376/mpb.2023.14.0009","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"531 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77904530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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