Pub Date : 2021-01-01DOI: 10.5376/MPB.2021.12.0019
Ningning Song, Huihui Liang, Yi An, Shenglong Bai, Feifei Ma, Zhen Zhang, Hao Li, Yun Zhou, Guang-yan Guo, Chunpeng Song
KNOTTED-like homeodomain ( KNOX ) gene family is a transcription factor encoding homeobox protein, which plays an important role in plant growth and morphogenesis. However, little information is available on the KNOX gene family in wheat ( Triticum aestivum L.). In this study, 36 KNOX genes with KNOX1 or KNOX2 domain distributed on 18 chromosomes were identified from wheat genome using bioinformatics methods. The phylogenetic evolution, gene structure, protein domains, cis-acting elements and gene expression patterns were analyzed in the present study. Based on the phylogenetic tree, the 36 TaKNOX genes were divided into two major subclasses, Class I and Class II, and the two subclasses were further divided into five evolutionary branches. Most TaKNOX genes contain four typical conserved protein domains: KNOX1, KNOX2, ELK and HOX. Some cis-acting elements are associated with hormonal, plant development and stress in TaKNOX promoters. The analysis result of transcriptome data from wheat different tissue showed that Class I KNOX genes had obvious tissue specificity, while Class II KNOX genes expressed widely in different wheat tissues. The study results provide important information for future analysis of the regulation and functions of the TaKNOX gene family.
{"title":"Identification and Bioinformatics Analysis of KNOX Gene Family in Wheat (Triticum aestivum L.)","authors":"Ningning Song, Huihui Liang, Yi An, Shenglong Bai, Feifei Ma, Zhen Zhang, Hao Li, Yun Zhou, Guang-yan Guo, Chunpeng Song","doi":"10.5376/MPB.2021.12.0019","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0019","url":null,"abstract":"KNOTTED-like homeodomain ( KNOX ) gene family is a transcription factor encoding homeobox protein, which plays an important role in plant growth and morphogenesis. However, little information is available on the KNOX gene family in wheat ( Triticum aestivum L.). In this study, 36 KNOX genes with KNOX1 or KNOX2 domain distributed on 18 chromosomes were identified from wheat genome using bioinformatics methods. The phylogenetic evolution, gene structure, protein domains, cis-acting elements and gene expression patterns were analyzed in the present study. Based on the phylogenetic tree, the 36 TaKNOX genes were divided into two major subclasses, Class I and Class II, and the two subclasses were further divided into five evolutionary branches. Most TaKNOX genes contain four typical conserved protein domains: KNOX1, KNOX2, ELK and HOX. Some cis-acting elements are associated with hormonal, plant development and stress in TaKNOX promoters. The analysis result of transcriptome data from wheat different tissue showed that Class I KNOX genes had obvious tissue specificity, while Class II KNOX genes expressed widely in different wheat tissues. The study results provide important information for future analysis of the regulation and functions of the TaKNOX gene family.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87201146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To explore the low temperature germination characteristics of rapeseed, 8 main cultivars were selected for germination experiments in incubators at 20°C, 16°C, 12°C and 8°C, respectively, to measure and analyze the germination potential, germination rate, root length, bud length, etc. The results showed that there was significant difference in the germination potential of tested rapeseeds at temperatures. And the low temperature of 8°C was significantly reduced the germination potential. With the decrease of germination temperature, the growth of roots and buds were inhibited significantly. There were significant differences both in temperatures and varieties. The germination potential, root length and bud length could be used as the main index to evaluate seed germination of rapeseed under low temperature. In addition, to screen varieties suitable for spring sowing as green manure, the spring sowing test was carried out. The study found that the fresh grass yield of “Huyouzao No.1” was 1712.53 kg/m2, which was much higher than other 7 varieties. Its relative root length was second only to “Huyou17” and its relative bud length next to “Huyoufei No.1” at 8°C, respectively. The results can provide a reference for early spring sowing of rapeseed as green manure.
{"title":"Tibet The Seed Germination Characteristics Under Low Temperature and Spring Sowing Study of 8 Rapeseed Varieties","authors":"Jifeng Zhu, Xirong Zhou, Jianxia Jiang, Junying Zhang, Li Yanli, Liyong Yang, Weirong Wang, Meiyan Jiang","doi":"10.5376/MPB.2021.12.0016","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0016","url":null,"abstract":"To explore the low temperature germination characteristics of rapeseed, 8 main cultivars were selected for germination experiments in incubators at 20°C, 16°C, 12°C and 8°C, respectively, to measure and analyze the germination potential, germination rate, root length, bud length, etc. The results showed that there was significant difference in the germination potential of tested rapeseeds at temperatures. And the low temperature of 8°C was significantly reduced the germination potential. With the decrease of germination temperature, the growth of roots and buds were inhibited significantly. There were significant differences both in temperatures and varieties. The germination potential, root length and bud length could be used as the main index to evaluate seed germination of rapeseed under low temperature. In addition, to screen varieties suitable for spring sowing as green manure, the spring sowing test was carried out. The study found that the fresh grass yield of “Huyouzao No.1” was 1712.53 kg/m2, which was much higher than other 7 varieties. Its relative root length was second only to “Huyou17” and its relative bud length next to “Huyoufei No.1” at 8°C, respectively. The results can provide a reference for early spring sowing of rapeseed as green manure.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81990067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5376/MPB.2021.12.0009
Ling-ling Xu, Yi Wang, Xiaolong Yuan, B. Lu, Jia-bo Hao, Yu Zhang, Jun-Hui Ma, Fei Wang
In order to grasp the germplasm resources of walnut in Yunnan province, the genetic diversity and relationship of 21 walnuts varieties commonly planted in Yunnan province were analyzed using SSR fluorescent labeling technology. The results showed that the average number of allelic loci of 12 pairs of primers used in 21 walnut species samples was 7.916 7. The mean number of alleles, Shannon Wiener index (I) and polymorphic information content (PIC) per locus were 4.3516, 1.643 6 and 0.705 5. Twelve pairs of polymorphism information content (PIC) were higher than 0.25, indicating that the 12 pairs of primers had good polymorphism in the samples. In the phylogenetic analysis, the 21 walnut varieties were divided into 7 subgroups, indicating that the 21 walnut varieties had rich genetic diversity, extensive gene exchange and complex genetic background in the breeding process.
{"title":"Genetic Diversity and Relationship Analysis of 21 Walnut Varieties Based on SSR Markers","authors":"Ling-ling Xu, Yi Wang, Xiaolong Yuan, B. Lu, Jia-bo Hao, Yu Zhang, Jun-Hui Ma, Fei Wang","doi":"10.5376/MPB.2021.12.0009","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0009","url":null,"abstract":"In order to grasp the germplasm resources of walnut in Yunnan province, the genetic diversity and relationship of 21 walnuts varieties commonly planted in Yunnan province were analyzed using SSR fluorescent labeling technology. The results showed that the average number of allelic loci of 12 pairs of primers used in 21 walnut species samples was 7.916 7. The mean number of alleles, Shannon Wiener index (I) and polymorphic information content (PIC) per locus were 4.3516, 1.643 6 and 0.705 5. Twelve pairs of polymorphism information content (PIC) were higher than 0.25, indicating that the 12 pairs of primers had good polymorphism in the samples. In the phylogenetic analysis, the 21 walnut varieties were divided into 7 subgroups, indicating that the 21 walnut varieties had rich genetic diversity, extensive gene exchange and complex genetic background in the breeding process.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76744519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5376/mpb.2021.12.0033
Jiajia Li, Xiangyi Li, Lei Wang, Shiping Wang
{"title":"The Regulation of Brassinolide Biosynthesis and Physiological Effects in Grape Berries","authors":"Jiajia Li, Xiangyi Li, Lei Wang, Shiping Wang","doi":"10.5376/mpb.2021.12.0033","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0033","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"26 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72448074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5376/mpb.2021.12.0029
Lei Zhou, Erjing Li, Huashan Xu, Zhijue Chen, Guocai Yang, Kai Liu, A. You
{"title":"Genotyping of 10 Grain Shape Genes in High Quality Xianindica Varieties by Molecular Marker","authors":"Lei Zhou, Erjing Li, Huashan Xu, Zhijue Chen, Guocai Yang, Kai Liu, A. You","doi":"10.5376/mpb.2021.12.0029","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0029","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77518007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5376/mpb.2020.11.0003
Yu-Huang Liao, Fangfang Wang, Xi Zhu, Ning Zhang, H. Si
Mitogen-activated protein kinase (MAPK) cascade reaction is one of important and complex signal networks involved in plant growth and development, hormone and stress response. As one of its main members, mitogen-activated protein kinase kinase (MAPKK) is located in the middle of the cascade reaction and plays a key role in signal collection and divergence. There were shown that potato ( Solanum tuberosum L.) StMAPKK1 (PGSC0003DMT400000744) gene responded to drought stress. Therefore, StMAPKK1 gene was firstly selected to screen its interacting protein. In this study, the bait vector pGBKT7-StMAPKK1 was constructed by homologous recombination and used to screen potato cDNA library by yeast ( Saccharomyces cerevisiae ) two-hybrid system. Five StMAPKK1-interacting proteins, hydrolase (hydrolyzing O-glycosyl compound), RING-H2 subgroup RHE protein, cyanate hydratase, ARF GTPase activator, and a C2 domain-containing protein, were obtained through this screening and were identificated by bioinformatics analysis, and the interaction was verified by small-scale hybridization verification. The results provided theoretical basis for further study on the signal pathway and biological function of potato StMAPKK1.
丝裂原活化蛋白激酶(Mitogen-activated protein kinase, MAPK)级联反应是参与植物生长发育、激素和胁迫反应的重要而复杂的信号网络之一。作为其主要成员之一，丝裂原活化蛋白激酶(MAPKK)位于级联反应的中间，在信号的收集和发散中起着关键作用。有研究表明马铃薯(Solanum tuberosum L.)StMAPKK1 (PGSC0003DMT400000744)基因响应干旱胁迫。因此，首先选择StMAPKK1基因筛选其相互作用蛋白。本研究通过同源重组构建诱饵载体pGBKT7-StMAPKK1，利用酵母双杂交系统对马铃薯cDNA文库进行筛选。筛选得到5个与stmapkk1相互作用的蛋白，分别是水解o -糖基化合物(hydrolase)、RING-H2亚群RHE蛋白、氰酸酯水合酶、ARF GTPase激活剂和一个C2结构域蛋白，通过生物信息学分析进行鉴定，并通过小规模杂交验证相互作用。该结果为进一步研究马铃薯StMAPKK1的信号通路和生物学功能提供了理论依据。
{"title":"Screening and Bioinformatics Analysis of Proteins Interacting with StMAPKK1 in Potato by Yeast Two-Hybrid System","authors":"Yu-Huang Liao, Fangfang Wang, Xi Zhu, Ning Zhang, H. Si","doi":"10.5376/mpb.2020.11.0003","DOIUrl":"https://doi.org/10.5376/mpb.2020.11.0003","url":null,"abstract":"Mitogen-activated protein kinase (MAPK) cascade reaction is one of important and complex signal networks involved in plant growth and development, hormone and stress response. As one of its main members, mitogen-activated protein kinase kinase (MAPKK) is located in the middle of the cascade reaction and plays a key role in signal collection and divergence. There were shown that potato ( Solanum tuberosum L.) StMAPKK1 (PGSC0003DMT400000744) gene responded to drought stress. Therefore, StMAPKK1 gene was firstly selected to screen its interacting protein. In this study, the bait vector pGBKT7-StMAPKK1 was constructed by homologous recombination and used to screen potato cDNA library by yeast ( Saccharomyces cerevisiae ) two-hybrid system. Five StMAPKK1-interacting proteins, hydrolase (hydrolyzing O-glycosyl compound), RING-H2 subgroup RHE protein, cyanate hydratase, ARF GTPase activator, and a C2 domain-containing protein, were obtained through this screening and were identificated by bioinformatics analysis, and the interaction was verified by small-scale hybridization verification. The results provided theoretical basis for further study on the signal pathway and biological function of potato StMAPKK1.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"2000 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88316396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5376/MPB.2021.12.0017
Tonghua Wang, Bao Li, Yiming Guo, Yaoting Zhang, Xinhong Liu, L. Fan, Lianghuan Qu, Li-Ming Deng, Mei Li
The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability of occurrence of maternal false hybrid due to the fertility of Polima CMS (Pol CMS) line greatly affected by the ambient temperature, therefore, the detection of maternal false hybrids is the key to purity identification. In present study, the SSR markers were designed based on the flanking sequences of Pol CMS restorer gene Rfp , and application of SSR markers in hybrid seed purity test of rapeseed. The main results were as follows: the sequences of Rfp were subjected to basic local alignment search tool queries against the Brassica rapa genome to determine chromosome positions, and 32 SSR markers were developed based on the upstream and downstream sequences of Rfp . Among the 32 SSR markers, one codominant marker was polymorphic between Pol CMS sterile and restorer line, the PCR product length was 162 bp in Pol CMS sterile lines, and 184 bp in restorer lines. Based on the PCR sequence, two insert fragments (15 bp and 7 bp) were identified in Pol CMS restorer lines. The SSR marker co-segregated with plant fertility of individuals in the “fengyou737” F 2 population. The purity of “fengyou737”, “fengyou320” and “fengyou958” were identified by means of PCR-SSR markers and field-planting, and the results of two ways were highly consistent, indicated that the codominant SSR marker tightly linked to Rfp , and the marker can detect paternal and maternal false hybrids. The present findings will provide technical assistance for hybrid seed purity test and genetic diversity analysis in rapeseed.
{"title":"Development of SSR Markers Associated with Polima CMS Fertility Restorer in Brassica napus L. and Its Application","authors":"Tonghua Wang, Bao Li, Yiming Guo, Yaoting Zhang, Xinhong Liu, L. Fan, Lianghuan Qu, Li-Ming Deng, Mei Li","doi":"10.5376/MPB.2021.12.0017","DOIUrl":"https://doi.org/10.5376/MPB.2021.12.0017","url":null,"abstract":"The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability of occurrence of maternal false hybrid due to the fertility of Polima CMS (Pol CMS) line greatly affected by the ambient temperature, therefore, the detection of maternal false hybrids is the key to purity identification. In present study, the SSR markers were designed based on the flanking sequences of Pol CMS restorer gene Rfp , and application of SSR markers in hybrid seed purity test of rapeseed. The main results were as follows: the sequences of Rfp were subjected to basic local alignment search tool queries against the Brassica rapa genome to determine chromosome positions, and 32 SSR markers were developed based on the upstream and downstream sequences of Rfp . Among the 32 SSR markers, one codominant marker was polymorphic between Pol CMS sterile and restorer line, the PCR product length was 162 bp in Pol CMS sterile lines, and 184 bp in restorer lines. Based on the PCR sequence, two insert fragments (15 bp and 7 bp) were identified in Pol CMS restorer lines. The SSR marker co-segregated with plant fertility of individuals in the “fengyou737” F 2 population. The purity of “fengyou737”, “fengyou320” and “fengyou958” were identified by means of PCR-SSR markers and field-planting, and the results of two ways were highly consistent, indicated that the codominant SSR marker tightly linked to Rfp , and the marker can detect paternal and maternal false hybrids. The present findings will provide technical assistance for hybrid seed purity test and genetic diversity analysis in rapeseed.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77236986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5376/mpb.2021.12.0036
Maohua Dai, Liying Liu, Y. Li, Chao Liu, Zhenliang Wu
{"title":"Breeding of Drought resistance and Salt tolerance, Suitable for Machine Picking New Cotton Variety Hengmian HD008","authors":"Maohua Dai, Liying Liu, Y. Li, Chao Liu, Zhenliang Wu","doi":"10.5376/mpb.2021.12.0036","DOIUrl":"https://doi.org/10.5376/mpb.2021.12.0036","url":null,"abstract":"","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72884708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：