Environmental and climatic fluctuations as well as abiotic stress factors affect agricultural production and cause a loss in quality and yield. It is important to find alternative solutions for the sustainability of agricultural production to feed the increasing population. Salt stress is one of the most devastating abiotic stress factors and tomato production is also affected by salt stress since it needs extensive irrigation for high yield. The exogenous application of some plant inducers showed promising results in the induction and improvement of plant tolerance to stress factors. Chitosan (2-amino-2-deoxy-b-D-glucosamine), one of the organic compounds, is getting significant attention in agriculture with its potential. Here, we evaluated the potential of chitosan application for salt stress tolerance on tomato. 0.03% and 0.05% chitosan solutions were applied as a foliar spray to the plant and salt tolerance improvement were investigated under untreated (0 mM NaCl) and 100 mM NaCl conditions. The growth-related (root and shoot diameters, above and below-ground biomass, number of leaves and branches, and plant height), photosynthetic parameters (chlorophyll a, b, total carotenoid content), and ion leakage were investigated. According to the results, chitosan application improves plant development in both untreated and salt-stress conditions and improved plant growth. Also, photosynthetic parameters showed that the application of chitosan increased chlorophyll contents under untreated conditions. Our result suggests that the application of chitosan may have a promising effect on salt stress tolerance and further research may shed light on its molecular mechanisms.
环境和气候波动以及非生物胁迫因素影响农业生产，造成质量和产量损失。重要的是为农业生产的可持续性寻找替代解决方案，以养活不断增长的人口。盐胁迫是最具破坏性的非生物胁迫因素之一，番茄生产也受到盐胁迫的影响，因为番茄需要大量灌溉才能高产。一些植物诱导剂的外源应用在诱导和提高植物对胁迫因子的耐受性方面显示出良好的效果。壳聚糖(2-氨基-2-脱氧-b- d -葡萄糖胺)是一种有机化合物，因其在农业上的巨大潜力而备受关注。本文对壳聚糖在番茄耐盐胁迫中的应用潜力进行了评价。以0.03%和0.05%的壳聚糖溶液作为叶面喷雾，在0 mM NaCl处理和100 mM NaCl处理下，考察了其耐盐性的提高。研究了生长相关指标(根、茎直径、地上、地下生物量、叶、枝数、株高)、光合参数(叶绿素a、b、类胡萝卜素总含量)和离子泄漏。结果表明，壳聚糖在未处理和盐胁迫条件下均能促进植物发育，促进植物生长。此外，光合参数表明，在未经处理的条件下，壳聚糖的施用增加了叶绿素含量。研究结果表明，壳聚糖的应用可能对盐胁迫耐受性有很好的影响，进一步的研究可能会揭示其分子机制。
{"title":"Alleviation of Salt Stress with Chitosan Foliar Application and Its Effects on Growth and Development in Tomato (Solanum lycopersicum L.)","authors":"N. Özkurt, Y. Bektas","doi":"10.19159/tutad.1168393","DOIUrl":"https://doi.org/10.19159/tutad.1168393","url":null,"abstract":"Environmental and climatic fluctuations as well as abiotic stress factors affect agricultural production and cause a loss in quality and yield. It is important to find alternative solutions for the sustainability of agricultural production to feed the increasing population. Salt stress is one of the most devastating abiotic stress factors and tomato production is also affected by salt stress since it needs extensive irrigation for high yield. The exogenous application of some plant inducers showed promising results in the induction and improvement of plant tolerance to stress factors. Chitosan (2-amino-2-deoxy-b-D-glucosamine), one of the organic compounds, is getting significant attention in agriculture with its potential. Here, we evaluated the potential of chitosan application for salt stress tolerance on tomato. 0.03% and 0.05% chitosan solutions were applied as a foliar spray to the plant and salt tolerance improvement were investigated under untreated (0 mM NaCl) and 100 mM NaCl conditions. The growth-related (root and shoot diameters, above and below-ground biomass, number of leaves and branches, and plant height), photosynthetic parameters (chlorophyll a, b, total carotenoid content), and ion leakage were investigated. According to the results, chitosan application improves plant development in both untreated and salt-stress conditions and improved plant growth. Also, photosynthetic parameters showed that the application of chitosan increased chlorophyll contents under untreated conditions. Our result suggests that the application of chitosan may have a promising effect on salt stress tolerance and further research may shed light on its molecular mechanisms.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48786864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xanthine oxidase (XO), in purine metabolism is a flavoprotein containing molybdenum with a key role. It has biological functions such as regeneration of NAD+, iron absorption and mobilization, reduction of nitrates. In this study, xanthine oxidase enzyme was purified by Sepharose-4B-L-tyrosine-4-aminobenzamidine dihydrochloride gel according to affinity chromatography technique and immobilization on glutaraldehyde was investigated. XO purified by ammonium sulfate precipitation and affinity chromatography was obtained with an 11.5 % yield and 694.04 degrees of purity. The purity of XO was confirmed by SDS-PAGE and a single band of around 150 kDa was observed. Kinetic constants (KM and VMax) of the enzyme were determined 1.67x10-4 M and 0.56 U/mL.min respectively by using xanthine compound as a substrate. The in vitro effects of NH4F, NH4Cl, CaCl2, ZnCl2, HgCl2, Hg(NO3)2.H2O compounds and commercially named colchicum dispert, commonly used in the treatment of gout disease in the clinic, were investigated. The IC50 values of compounds showing inhibition effect were determined. Afterward XO was immobilized on glutaraldehyde, which was used as a solid support material. The highest XO activity was observed in the sample of the immobilized enzyme at a rate of 6 % glutaraldehyde. The kinetic constants (KM and VMax) of the immobilized enzyme were determined as 5.18x10-4 M and 0.73 U/mL.min respectively. These values revealed that the catalytic activity of the free enzyme was higher than the immobilized enzyme.
{"title":"Purification of Xanthine Oxidase and Investigation of Its Immobilization with Glutaraldehyde","authors":"Yeşim Kaya, S. Işık, S. Uzunoğlu, M. Kaya","doi":"10.19159/tutad.1084383","DOIUrl":"https://doi.org/10.19159/tutad.1084383","url":null,"abstract":"Xanthine oxidase (XO), in purine metabolism is a flavoprotein containing molybdenum with a key role. It has biological functions such as regeneration of NAD+, iron absorption and mobilization, reduction of nitrates. In this study, xanthine oxidase enzyme was purified by Sepharose-4B-L-tyrosine-4-aminobenzamidine dihydrochloride gel according to affinity chromatography technique and immobilization on glutaraldehyde was investigated. XO purified by ammonium sulfate precipitation and affinity chromatography was obtained with an 11.5 % yield and 694.04 degrees of purity. The purity of XO was confirmed by SDS-PAGE and a single band of around 150 kDa was observed. Kinetic constants (KM and VMax) of the enzyme were determined 1.67x10-4 M and 0.56 U/mL.min respectively by using xanthine compound as a substrate. The in vitro effects of NH4F, NH4Cl, CaCl2, ZnCl2, HgCl2, Hg(NO3)2.H2O compounds and commercially named colchicum dispert, commonly used in the treatment of gout disease in the clinic, were investigated. The IC50 values of compounds showing inhibition effect were determined. Afterward XO was immobilized on glutaraldehyde, which was used as a solid support material. The highest XO activity was observed in the sample of the immobilized enzyme at a rate of 6 % glutaraldehyde. The kinetic constants (KM and VMax) of the immobilized enzyme were determined as 5.18x10-4 M and 0.73 U/mL.min respectively. These values revealed that the catalytic activity of the free enzyme was higher than the immobilized enzyme.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67783362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışma, sorgum tohumlarının GA3 ile muamale edilmesinin tuzlu koşullarda çimlenme ve fide gelişimine etkilerini belirlemek amacıyla çimlendirme dolabında, petri kapları içerisinde yürütülmüştür. Çalışmada, tohumlar 4 farklı GA3 dozunda (saf su, 100, 200, 300 ppm GA3) 24 saat bekletilmişler ve daha sonra 5 farklı tuz dozunda (saf su, 2500, 5000, 7500, 10000 ppm NaCl) çimlendirilmiştir. Çalışma bölünmüş parseller deneme deseninde 3 tekrarlamalı olarak kurulmuştur. Çalışmada, çimlenme oranı, sürgün uzunluğu, kök uzunluğu, sürgün ve kök yaş ağırlıkları ile sürgün ve kök kuru ağırlıkları incelenmiştir. Tuz ve GA3 uygulamalarının çimlenme oranı dışında incelenen diğer tüm özellikler üzerindeki etkileri istatistiki açıdan önemli bulunmuştur. Tuz*GA3 interaksiyon etkisi önemsiz bulunmakla beraber yapılan çoklu karşılaştırmalarda (Tukey) ortalamalar arasında önemli farklar ortaya çıkmıştır. Artan tuz dozunun çimlenme oranı dışındaki tüm özellikleri olumsuz etkilediği belirlenmiştir. GA3 uygulamalarıda, 200 ve 300 ppm GA3 dozlarının sürgün uzunluğunda tuzluluğun neden olduğu zararı hafiflettiği, ancak diğer özelliklerde bu etkiyi göstermediği tespit edilmiştir.
为了确定生产的影响和反馈的发展，在汽油门内进行了研究，以评估处理GA3的有害条件。在工作中，预期种子在4种不同的GA3剂量（纯水，100200300ppm GA3）下持续24小时，然后在5种不同的盐剂量（纯水、250050007500、10000ppm NaCl）下。尝试测试分区时重新生成了工作表。Çalışmada、çimlenme oranı、sürgün uzunluğu、kök uzunluå。Tuz ve GA3维吾尔语malarınınçimlenme oranıdışında incelenen diğer tümözelliklerüzerindeki etkileri istatistiki açıdanönemli bulunmuştur。Tukey*GA3相互作用对多重比较的影响产生了不相关的影响。此外，在盐剂量的分组速率之外的所有性质都是不希望的。在GA3应用中，200和300ppm的GA3剂量在延长的持续时间内降低了轨迹损伤的风险，但其他特征表明没有显示出这种效果。
{"title":"Farklı Gibberellik Asit (GA3) Uygulamalarının Farklı Tuz Yoğunluklarında Sorgum Bitkisinin (Sorghum Bicolor (L.) Moench) Çimlenme ve Fide Gelişimi Üzerine Etkileri","authors":"Cebrail Yildirim, Merve Başak, Bilal Aydinoğlu","doi":"10.19159/tutad.1128902","DOIUrl":"https://doi.org/10.19159/tutad.1128902","url":null,"abstract":"Bu çalışma, sorgum tohumlarının GA3 ile muamale edilmesinin tuzlu koşullarda çimlenme ve fide gelişimine etkilerini belirlemek amacıyla çimlendirme dolabında, petri kapları içerisinde yürütülmüştür. Çalışmada, tohumlar 4 farklı GA3 dozunda (saf su, 100, 200, 300 ppm GA3) 24 saat bekletilmişler ve daha sonra 5 farklı tuz dozunda (saf su, 2500, 5000, 7500, 10000 ppm NaCl) çimlendirilmiştir. Çalışma bölünmüş parseller deneme deseninde 3 tekrarlamalı olarak kurulmuştur. Çalışmada, çimlenme oranı, sürgün uzunluğu, kök uzunluğu, sürgün ve kök yaş ağırlıkları ile sürgün ve kök kuru ağırlıkları incelenmiştir. Tuz ve GA3 uygulamalarının çimlenme oranı dışında incelenen diğer tüm özellikler üzerindeki etkileri istatistiki açıdan önemli bulunmuştur. Tuz*GA3 interaksiyon etkisi önemsiz bulunmakla beraber yapılan çoklu karşılaştırmalarda (Tukey) ortalamalar arasında önemli farklar ortaya çıkmıştır. Artan tuz dozunun çimlenme oranı dışındaki tüm özellikleri olumsuz etkilediği belirlenmiştir. GA3 uygulamalarıda, 200 ve 300 ppm GA3 dozlarının sürgün uzunluğunda tuzluluğun neden olduğu zararı hafiflettiği, ancak diğer özelliklerde bu etkiyi göstermediği tespit edilmiştir.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışmada, Şanlıurfa ekolojik şartlarında 2. ürün olarak yetiştirilen tatlı sorgum (Sorghum Bicolor var. saccharatum (L.) Mohlenbr.) genotiplerinin saplarının özsuyu alındıktan sonraya geriye kalan küspesinden elde edilen peletlerin bazı fiziksel özellikleri saptanmıştır. Çalışmada, hasat edilen bitkilerin sapları sıkılarak suyu alındıktan sonra geriye kalan küspe kurutularak pelet yapılmıştır. Elde edilen peletlerde, pelet kalitesini etkileyen nem içeriği, dayanıklılık direnci, yığın yoğunluğu, parça yoğunluğu, sertlik ve nem alma direnci gibi fiziksel özellikler belirlenmiştir. Çalışmanın iki yıllık ortalama sonuçlarına göre, nem içeriğinin % 3.44-9.48, dayanıklılık direncinin % 94.6-98.9, yığın yoğunluğunun 488-724 kg m-3, parça yoğunluğunun 1039-1305 kg m-3 ve nem alma direncinin % 9.98-16.60 değerleri arasında değiştiği belirlenmiştir. Sertlik direnci ise, genotiplere göre farklılık göstermiş olup 2016 yılında ortalama 3770 N, 2017 yılında ise ortalama 1908 N olarak belirlenmiştir. Elde edilen verilere bakıldığında, tatlı sorgum küspesinden yapılan peletler, ortam koşullarından etkilenmeyen, taşınması ve depolaması kolay yenilenebilir bir enerji kaynağı olarak değerlendirilebilir.
{"title":"Şanlıurfa Şartlarında Farklı Sorgum (Sorghum Bicolor var. saccharatum (L.) Mohlenbr.) Genotiplerinin Posasından Yapılan Peletlerin Bazı Fiziksel Özelliklerinin Belirlenmesi","authors":"Mahmut Dok, Mine Aksoy, Ayşegül Çeli̇k, C. Yücel","doi":"10.19159/tutad.1127371","DOIUrl":"https://doi.org/10.19159/tutad.1127371","url":null,"abstract":"Bu çalışmada, Şanlıurfa ekolojik şartlarında 2. ürün olarak yetiştirilen tatlı sorgum (Sorghum Bicolor var. saccharatum (L.) Mohlenbr.) genotiplerinin saplarının özsuyu alındıktan sonraya geriye kalan küspesinden elde edilen peletlerin bazı fiziksel özellikleri saptanmıştır. Çalışmada, hasat edilen bitkilerin sapları sıkılarak suyu alındıktan sonra geriye kalan küspe kurutularak pelet yapılmıştır. Elde edilen peletlerde, pelet kalitesini etkileyen nem içeriği, dayanıklılık direnci, yığın yoğunluğu, parça yoğunluğu, sertlik ve nem alma direnci gibi fiziksel özellikler belirlenmiştir. Çalışmanın iki yıllık ortalama sonuçlarına göre, nem içeriğinin % 3.44-9.48, dayanıklılık direncinin % 94.6-98.9, yığın yoğunluğunun 488-724 kg m-3, parça yoğunluğunun 1039-1305 kg m-3 ve nem alma direncinin % 9.98-16.60 değerleri arasında değiştiği belirlenmiştir. Sertlik direnci ise, genotiplere göre farklılık göstermiş olup 2016 yılında ortalama 3770 N, 2017 yılında ise ortalama 1908 N olarak belirlenmiştir. Elde edilen verilere bakıldığında, tatlı sorgum küspesinden yapılan peletler, ortam koşullarından etkilenmeyen, taşınması ve depolaması kolay yenilenebilir bir enerji kaynağı olarak değerlendirilebilir.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study presents the possibility of utilizing a passive solar system in sunny places, which collects solar energy by using polyethylene sheet to trap the long-wave thermal radiation and store it in water for warming the internal environment of beehives and its effect on nest temperatures, honey area, pollen area, sealed brood area, and the number of occupied frames. A number of six Langstroth hives containing honeybee colonies of equal strength from the species of hybrid Carniolan are divided into two groups as follows: a control group (untreated hive), and modified beehives that have been treated with the solar energy storage system. The solar energy storage system consists of an insulated wooden drawer located under the beehive's bottom board, containing sealed water bags, and is covered with polyethylene sheet. Solar energy stored in water is used to reduce the variation of inside air temperatures between daylight and night time. The highest values of the honey area, pollen area, sealed brood area, and the number of occupied frames (916 cm2, 842 cm2, 3688 cm2, and 9 frames, respectively) were shown for the modified beehives at the end of March, while the lowest values (98 cm2, 219 cm2, 911 cm2, and 3 frames, respectively) were recorded for the control groups of beehives at January, respectively. In modified beehives treated with a solar energy storage system, there was a significant rise in hive temperature, honey area, pollen area, sealed brood area, and bee population. So, it is recommended to use the new modification for warming beehives.
{"title":"Warming beehives with solar energy stored in water","authors":"M. AL-RAJHİ","doi":"10.19159/tutad.1126564","DOIUrl":"https://doi.org/10.19159/tutad.1126564","url":null,"abstract":"This study presents the possibility of utilizing a passive solar system in sunny places, which collects solar energy by using polyethylene sheet to trap the long-wave thermal radiation and store it in water for warming the internal environment of beehives and its effect on nest temperatures, honey area, pollen area, sealed brood area, and the number of occupied frames. A number of six Langstroth hives containing honeybee colonies of equal strength from the species of hybrid Carniolan are divided into two groups as follows: a control group (untreated hive), and modified beehives that have been treated with the solar energy storage system. The solar energy storage system consists of an insulated wooden drawer located under the beehive's bottom board, containing sealed water bags, and is covered with polyethylene sheet. Solar energy stored in water is used to reduce the variation of inside air temperatures between daylight and night time. The highest values of the honey area, pollen area, sealed brood area, and the number of occupied frames (916 cm2, 842 cm2, 3688 cm2, and 9 frames, respectively) were shown for the modified beehives at the end of March, while the lowest values (98 cm2, 219 cm2, 911 cm2, and 3 frames, respectively) were recorded for the control groups of beehives at January, respectively. In modified beehives treated with a solar energy storage system, there was a significant rise in hive temperature, honey area, pollen area, sealed brood area, and bee population. So, it is recommended to use the new modification for warming beehives.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Palacıoğlu, G. Özer, M. Yeken, E. Ören, V. Çiftçi, H. Bayraktar
Angular leaf spot caused by Pseudocercospora griseola is a common disease in all common bean production areas of the world. The breeding and use of resistant varieties are effective and sustainable methods of combating this pathogen. In this context, different resistance genes against the pathogen were explored, and many molecular markers associated with these genes were widely used in breeding studies. This research investigated resistance sources to angular leaf spot on 64 bean varieties widely grown in Turkey using SN02, SH13, and g2303 molecular markers. The findings revealed that the most common gene among the varieties was Phg-2, followed by Phg-ON and Phg-1 genes, respectively. Concerning different gene combinations, it was determined that 17 cultivars had 1 gene, 41 cultivars had 2 genes, and 5 cultivars (Yalova-17, Boncuk, Aslan, Helda, Akman-98) had all three resistance genes. The determined resistance genes may contribute to the breeding studies to be carried out on angular leaf spot.
{"title":"Screening of Turkish Common Bean Varieties for Resistance Sources to Angular Leaf Spot Disease","authors":"G. Palacıoğlu, G. Özer, M. Yeken, E. Ören, V. Çiftçi, H. Bayraktar","doi":"10.19159/tutad.1126620","DOIUrl":"https://doi.org/10.19159/tutad.1126620","url":null,"abstract":"Angular leaf spot caused by Pseudocercospora griseola is a common disease in all common bean production areas of the world. The breeding and use of resistant varieties are effective and sustainable methods of combating this pathogen. In this context, different resistance genes against the pathogen were explored, and many molecular markers associated with these genes were widely used in breeding studies. This research investigated resistance sources to angular leaf spot on 64 bean varieties widely grown in Turkey using SN02, SH13, and g2303 molecular markers. The findings revealed that the most common gene among the varieties was Phg-2, followed by Phg-ON and Phg-1 genes, respectively. Concerning different gene combinations, it was determined that 17 cultivars had 1 gene, 41 cultivars had 2 genes, and 5 cultivars (Yalova-17, Boncuk, Aslan, Helda, Akman-98) had all three resistance genes. The determined resistance genes may contribute to the breeding studies to be carried out on angular leaf spot.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48872104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmed Sidar Aygören, Recep Aydinyurt, Sümeyra Uçar, Ayşe Gül Kasapoğlu, E. Yaprak, Burak Öner, Selman Muslu, Murat Isiyel, Emre Ilhan, M. Aydın, M. Turan
Plant growth and development are regulated by light, which is a significant environmental component. It is involved in seedling de-etiolation, phototropism, shadow escaping, seed germination, circadian rhythms, and blooming timing, among other reactions in the plant life cycle (collectively termed photomorphogenesis). These light responses are controlled by phytochromes, which interact with a variety of partner proteins. The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in the response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.
{"title":"Fasulyede Tuz ve Kuraklık Stresi Altında PIF Gen Ailesinin Genom Çapında Analizi ve Karakterizasyonu","authors":"Ahmed Sidar Aygören, Recep Aydinyurt, Sümeyra Uçar, Ayşe Gül Kasapoğlu, E. Yaprak, Burak Öner, Selman Muslu, Murat Isiyel, Emre Ilhan, M. Aydın, M. Turan","doi":"10.19159/tutad.1109558","DOIUrl":"https://doi.org/10.19159/tutad.1109558","url":null,"abstract":"Plant growth and development are regulated by light, which is a significant environmental component. It is involved in seedling de-etiolation, phototropism, shadow escaping, seed germination, circadian rhythms, and blooming timing, among other reactions in the plant life cycle (collectively termed photomorphogenesis). These light responses are controlled by phytochromes, which interact with a variety of partner proteins. The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in the response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oğuzhan Özdemir, Nurten Yilmaz, Mesut Gok, M. Kaya
This study aims to evaluate the volatile oil components by headspace gas chromatography/mass spectroscopy (headspace/GC-MS) analysis, the antimicrobial effect on some gram-negative/positive pathogenic microorganisms and lactic acid bacteria (by agar well diffusion, MIC test) and antioxidant capacity (DPPH, metal chelating, FRAP) of the oil extracted from Lavandula angustifolia (LA) by the clevenger method. As a result of the analysis, it was determined by headspace/GC-MS analysis that eucalyptol was the highest with the value of 22.58%, α-linalol was the second with the value of 19.44% and linalyl acetate was 14.61%. Lavandulol, essential component of Lavender, was determined by 1.39%. The highest antimicrobial effect of LA oil was found on Bacillus subtilus and MRSA (zone diameter of 24 to 22 mm, respectively), following on Staph. aureus. It was found that antibacterial effects on Staph. aureus and E. coli, Campliobacter jejuni, Salmonella poona, and lactic acid bacteria such as Lactobacillus reuteri and Lactobacillus plantarum (17, 16, 14, 14, 12, 12, 11 mm zone diameter). Therewithal, a significant antioxidative effect was determined on DPPH, FRAP and metal chelating analyzes. Also, the importance of its use in complementary medical and aroma therapy for preventive and symptomatic treatment of diseases. It is thought that present research will shed light on in vitro/in vivo studies to be carried out with LA oil.
{"title":"Novel viewpoint by headspace/GC–MS: Volatile Oil of Lavandula angustifolia origin of Denizli Provience","authors":"Oğuzhan Özdemir, Nurten Yilmaz, Mesut Gok, M. Kaya","doi":"10.19159/tutad.1099620","DOIUrl":"https://doi.org/10.19159/tutad.1099620","url":null,"abstract":"This study aims to evaluate the volatile oil components by headspace gas chromatography/mass spectroscopy (headspace/GC-MS) analysis, the antimicrobial effect on some gram-negative/positive pathogenic microorganisms and lactic acid bacteria (by agar well diffusion, MIC test) and antioxidant capacity (DPPH, metal chelating, FRAP) of the oil extracted from Lavandula angustifolia (LA) by the clevenger method. As a result of the analysis, it was determined by headspace/GC-MS analysis that eucalyptol was the highest with the value of 22.58%, α-linalol was the second with the value of 19.44% and linalyl acetate was 14.61%. Lavandulol, essential component of Lavender, was determined by 1.39%. The highest antimicrobial effect of LA oil was found on Bacillus subtilus and MRSA (zone diameter of 24 to 22 mm, respectively), following on Staph. aureus. It was found that antibacterial effects on Staph. aureus and E. coli, Campliobacter jejuni, Salmonella poona, and lactic acid bacteria such as Lactobacillus reuteri and Lactobacillus plantarum (17, 16, 14, 14, 12, 12, 11 mm zone diameter). Therewithal, a significant antioxidative effect was determined on DPPH, FRAP and metal chelating analyzes. Also, the importance of its use in complementary medical and aroma therapy for preventive and symptomatic treatment of diseases. It is thought that present research will shed light on in vitro/in vivo studies to be carried out with LA oil.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Çalışma, klorür uygulamalarının şeker pancarı (Beta vulgaris L.) verim ve kalite değerlerine etkisini belirlemek amacıyla 2018 ve 2019 yıllarında Şeker Enstitüsü Eskişehir Deneme İstasyonunda yapılmıştır. Araştırmada şeker pancarının(Beta vulgaris L.) verim ve kalite değerlerinin ölçülmesi yanında uygulamaların bitki besin maddesi kapsamına etkisi değerlendirilmiştir. Denemeler her iki yılda da tesadüf blokları deneme desenine göre 4 tekrarlamalı olarak yürütülmüştür. Araştırma konuları klorürün 0.0, 2.0, 4.0, 6.0 ve 8.0 kg Cl da-1 uygulama düzeylerinden oluşmuştur. Birleştirilmiş varyans analiz sonuçlarına göre klorür; pancar kök verimi, şeker varlığı, sodyum kapsamı, potasyum kapsamı, zararlı azot kapsamı, arıtılmış şeker varlığı ve şeker verimi üzerine anlamlı bir etki yapmamıştır. Şeker pancarı verim ve kalite değerlerini artırmayan potasyum klorür kaynaklı klorür uygulamaları; bitkinin verim ve kalitesini düşürecek toksik bir etkide yapmamıştır. Klorürün uygulamaları ile verilen 0.0-16.8 kg da-1 arasındaki potasyum klorür gübre uygulanması orta sınıf klorür kapsayan deneme alanı topraklarında şeker pancarına toksik etki yapmadığı, verim ve kalitede değerlerinde de düşüşe neden olmadığı görülmüştür. Ayrıca klorür uygulamalarının bitkinin nitrat alımını engelleyerek azot kapsamını düşürmesiyle şeker pancarının şeker varlığında artış gerçekleşmesi stratejisinin çalışmadığı görülmüştür. Uygulamalar; şeker pancarı makro ve mikro bikri besin maddelerinden bitki N, P, K, Mg, S, Zn, Fe, Mn , Cu, ve B kapsamını değiştirmezken Ca ve Cl miktarını önemli ölçüde artırmıştır.
糖煎饼（Beta vulgaris L.）于2018年和2019年完成，以确定对质量数据和质量值的影响。Araştırmadaşeker pancarının（Beta vulgaris L.）是一位维吾尔族人，他是一位名叫kapsamına etkisi değerlendirilmiştir的女性。根据随机区块的调查，这些试验每两年重复四次。研究问题包括0.0、2.0、4.0、6.0和8.0 kg Cl da-1应用级别。合并方差遵循分析结果；人参根数据、含糖量、钠含量、钾含量、受损油含量、加糖含量和含糖量数据没有显著影响。钾氯应用不会增加糖煎饼和质量价值；该植物的数据和质量没有影响毒性作用。通过应用颜色给出的0.0至16.8 kg和1之间的氯化钾的使用表明，在氯化碳的中心类别、数据和质量值中，对糖潘卡拉没有毒性影响。人们还发现，增加糖煎饼的策略不起作用，阻止了植物的硝酸盐含量降低。应用程序；şeker pancarımakro ve mikro bikri besin maddelerinden bitki N，P，K，Mg，S，Zn，Fe，Mn，Cu，ve B kapsamıNıdeğiştirmezken Ca ve Cl miktarıNönemliölçüde artırmıştır。
{"title":"Klorürün Şeker Pancarı Verim ve Kalite Değerleri Üzerine Etkisi","authors":"A. Pişkin","doi":"10.19159/tutad.1079341","DOIUrl":"https://doi.org/10.19159/tutad.1079341","url":null,"abstract":"Çalışma, klorür uygulamalarının şeker pancarı (Beta vulgaris L.) verim ve kalite değerlerine etkisini belirlemek amacıyla 2018 ve 2019 yıllarında Şeker Enstitüsü Eskişehir Deneme İstasyonunda yapılmıştır. Araştırmada şeker pancarının(Beta vulgaris L.) verim ve kalite değerlerinin ölçülmesi yanında uygulamaların bitki besin maddesi kapsamına etkisi değerlendirilmiştir. Denemeler her iki yılda da tesadüf blokları deneme desenine göre 4 tekrarlamalı olarak yürütülmüştür. Araştırma konuları klorürün 0.0, 2.0, 4.0, 6.0 ve 8.0 kg Cl da-1 uygulama düzeylerinden oluşmuştur. Birleştirilmiş varyans analiz sonuçlarına göre klorür; pancar kök verimi, şeker varlığı, sodyum kapsamı, potasyum kapsamı, zararlı azot kapsamı, arıtılmış şeker varlığı ve şeker verimi üzerine anlamlı bir etki yapmamıştır. Şeker pancarı verim ve kalite değerlerini artırmayan potasyum klorür kaynaklı klorür uygulamaları; bitkinin verim ve kalitesini düşürecek toksik bir etkide yapmamıştır. Klorürün uygulamaları ile verilen 0.0-16.8 kg da-1 arasındaki potasyum klorür gübre uygulanması orta sınıf klorür kapsayan deneme alanı topraklarında şeker pancarına toksik etki yapmadığı, verim ve kalitede değerlerinde de düşüşe neden olmadığı görülmüştür. Ayrıca klorür uygulamalarının bitkinin nitrat alımını engelleyerek azot kapsamını düşürmesiyle şeker pancarının şeker varlığında artış gerçekleşmesi stratejisinin çalışmadığı görülmüştür. Uygulamalar; şeker pancarı makro ve mikro bikri besin maddelerinden bitki N, P, K, Mg, S, Zn, Fe, Mn , Cu, ve B kapsamını değiştirmezken Ca ve Cl miktarını önemli ölçüde artırmıştır.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67783312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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