Pub Date : 2023-05-04DOI: 10.29413/abs.2023-8.2.14
I. Pendyurin, I. Vasilyev, I. S. Kopylov
The aim. To analyze the results of surgical treatment of sacral schwannomas with their extension into lower pelvis.Materials and methods. We analyzed the clinical results of surgical treatment of 25 patients with sacral schwannomas, including those with ventral extension and growth into pelvic cavity. All patients were operated at the Clinic of Neurosurgery of the Novosibirsk Research Institute of Traumatology and Orthopedics named after Ya.L. Tsivyan for a 10-year period. The analysis of literature describing prevalence, classification, diagnosis and surgical treatment of sacral tumors and schwannomas in particular was carried out. The features of the course, diagnosis and surgical treatment of sacral schwannomas and accepted surgical methods for their treatment are described.Results. The average follow-up period of the patients in our study was 61.6 ± 2 months. Compared with the initial indicators of patients in the postoperative period (1 year after the surgery), a good clinical result with the regression of symptoms was registered in 17 (68 %) out of 25 patients, and 10 (40 %) of them had a significant regression of neurological disorders. 3 (12 %) operated patients had a deterioration of neurological disorders in the form of aggravation of sensitive disorders; one patient had an aggravation of pelvic organ dysfunction. Radical removal of the tumor was achieved in 24 (96 %) out of 25 cases; subtotal resection was performed in 1 (4 %) patient. The number of relapses and continued growth of tumors in our series was 12 % (3 out of 25 patients). The mortality rate was 0 %. The complications were registered in 3 (12 %) cases and manifested as wound liquorrhea in cases of using posterior approach.Conclusion. The degree of radicality of tumor resection and clinical outcomes is directly related to the selected surgical approach.
{"title":"The results of surgical treatment of sacral schwannomas with extension into pelvic cavity","authors":"I. Pendyurin, I. Vasilyev, I. S. Kopylov","doi":"10.29413/abs.2023-8.2.14","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.14","url":null,"abstract":"The aim. To analyze the results of surgical treatment of sacral schwannomas with their extension into lower pelvis.Materials and methods. We analyzed the clinical results of surgical treatment of 25 patients with sacral schwannomas, including those with ventral extension and growth into pelvic cavity. All patients were operated at the Clinic of Neurosurgery of the Novosibirsk Research Institute of Traumatology and Orthopedics named after Ya.L. Tsivyan for a 10-year period. The analysis of literature describing prevalence, classification, diagnosis and surgical treatment of sacral tumors and schwannomas in particular was carried out. The features of the course, diagnosis and surgical treatment of sacral schwannomas and accepted surgical methods for their treatment are described.Results. The average follow-up period of the patients in our study was 61.6 ± 2 months. Compared with the initial indicators of patients in the postoperative period (1 year after the surgery), a good clinical result with the regression of symptoms was registered in 17 (68 %) out of 25 patients, and 10 (40 %) of them had a significant regression of neurological disorders. 3 (12 %) operated patients had a deterioration of neurological disorders in the form of aggravation of sensitive disorders; one patient had an aggravation of pelvic organ dysfunction. Radical removal of the tumor was achieved in 24 (96 %) out of 25 cases; subtotal resection was performed in 1 (4 %) patient. The number of relapses and continued growth of tumors in our series was 12 % (3 out of 25 patients). The mortality rate was 0 %. The complications were registered in 3 (12 %) cases and manifested as wound liquorrhea in cases of using posterior approach.Conclusion. The degree of radicality of tumor resection and clinical outcomes is directly related to the selected surgical approach.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84564526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.29413/abs.2023-8.2.22
A. N. Zharikov, V. Lubyanskiy, A. Aliev, V. Seroshtanov, K. E. Vlasov
Background. Enteroatmospheric fistulas (EAF) that occur during the use of the “open abdomen” surgical tactics are a complex surgical pathology with a high mortality rate.The aim. To assess the effectiveness of treatment of various forms of enteroatmospheric fistulas in patients with postoperative peritonitis using vacuum aspiration technology.Methods. We assessed the results of the surgical treatment of 46 patients with EAF in the late course of postoperative peritonitis (PP). Three clinical and morphological groups were distinguished: group 1 (n = 24) – EAF in small wounds of the anterior abdominal wall; group 2 (n = 15) – EAF opening into limited cavities; group 3 (n = 7) – EAF opening into laparostoma wounds. In group 1, a fistula was formed using continuous aspiration devices or VAC systems. In group 2, we used continuous aspiration of intestinal contents from the cavity. In group 3, laparostoma was treated using vacuum devices with isolation of the intestinal fistula and simulation of a floating enterostoma.Results. Group 3 of patients with EAF was characterized by a high flow rate (1224.2 ± 210.3 ml), duration of treatment (87.3 ± 12.5 day), extensive laparostoma (335.4 ± 14.3 сm2), high mortality rate (57.1 %). The best results of treatment were obtained in groups 1 and 2. The flow rate was 675.8 ± 154.3 and 541.3 ± 114.1 ml, the duration of treatment was 2 or 3 times less (37.7 ± 6.1 and 26.4 ± 5.2 days), the mortality rate was 8.3 % and 6.7 % respectively.Conclusion. EAF that occur when using the “open abdomen” surgical tactics due to the impossibility of their isolation in extensive wounds of the anterior abdominal wall are complicated clinical and morphological forms. For their treatment, it is advisable to use VAC systems, aimed at the treatment of both the anterior abdominal wall wound itself and the intestinal fistula opening into it for its gradual extraterritorialization by modeling a floating enterostoma in a vacuum device.
{"title":"Experience in surgical treatment of enteroatmospheric fistulas in the late period of postoperative peritonitis","authors":"A. N. Zharikov, V. Lubyanskiy, A. Aliev, V. Seroshtanov, K. E. Vlasov","doi":"10.29413/abs.2023-8.2.22","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.22","url":null,"abstract":"Background. Enteroatmospheric fistulas (EAF) that occur during the use of the “open abdomen” surgical tactics are a complex surgical pathology with a high mortality rate.The aim. To assess the effectiveness of treatment of various forms of enteroatmospheric fistulas in patients with postoperative peritonitis using vacuum aspiration technology.Methods. We assessed the results of the surgical treatment of 46 patients with EAF in the late course of postoperative peritonitis (PP). Three clinical and morphological groups were distinguished: group 1 (n = 24) – EAF in small wounds of the anterior abdominal wall; group 2 (n = 15) – EAF opening into limited cavities; group 3 (n = 7) – EAF opening into laparostoma wounds. In group 1, a fistula was formed using continuous aspiration devices or VAC systems. In group 2, we used continuous aspiration of intestinal contents from the cavity. In group 3, laparostoma was treated using vacuum devices with isolation of the intestinal fistula and simulation of a floating enterostoma.Results. Group 3 of patients with EAF was characterized by a high flow rate (1224.2 ± 210.3 ml), duration of treatment (87.3 ± 12.5 day), extensive laparostoma (335.4 ± 14.3 сm2), high mortality rate (57.1 %). The best results of treatment were obtained in groups 1 and 2. The flow rate was 675.8 ± 154.3 and 541.3 ± 114.1 ml, the duration of treatment was 2 or 3 times less (37.7 ± 6.1 and 26.4 ± 5.2 days), the mortality rate was 8.3 % and 6.7 % respectively.Conclusion. EAF that occur when using the “open abdomen” surgical tactics due to the impossibility of their isolation in extensive wounds of the anterior abdominal wall are complicated clinical and morphological forms. For their treatment, it is advisable to use VAC systems, aimed at the treatment of both the anterior abdominal wall wound itself and the intestinal fistula opening into it for its gradual extraterritorialization by modeling a floating enterostoma in a vacuum device.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88142220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.29413/abs.2023-8.2.21
V. Panteleev, M. Nartaylakov, I. Z. Salimgareev, A. Petrov
Rationale. Alveococcosis is a rare disease, its diagnosis and treatment depend on surgical techniques, equipment and clinical experience. The aim. To develop a diagnostic algorithm and compare the results of surgical treatment of patients with liver alveococcosis in different periods of time.Materials and methods. At the first stage, we carried out a retrospective analysis (1995–2007) of 33 patients with alveococcosis (a comparison group). At the second stage, a prospective clinical study (2008–2021) was performed on 39 patients (the main group). The number of patients was determined in accordance with the inclusion and exclusion criteria, and the study groups were comparable in age, sex, parasite localization (p > 0.05). For the names of operations, the WHO classification of alveococcosis was used.Results. In the main group, there is an increase in the applicability of: enzyme immunoassay; ultrasound and computed tomography; biopsy. Complications decreased by 2.7 times from 54.6 % in the comparison group to 20.6 % in the main group (χ2 = 8.97; df = 1; p = 0.003). The average duration of operations, as well as the average volume of blood loss in the comparison group and the main group were, respectively: with atypical resection – 220.4 and 180.2 min (p = 0.003), 640.1 and 480.0 ml (p = 0.005); with anatomical resection – 296.2 and 247.2 min (p = 0.002), 1450.2 and 1150.3 ml (p = 0.018); with cytoreductive resection – 230.2 and 200.1 min (p = 0.004), 860.3 and 670.4 ml (p = 0.001). There were 13 (39 %) cytoreductive resections in the comparison group, and 3 (8 %) in the main group (χ2 = 4.74; df = 1; p = 0.029).Conclusion. Timely diagnosis of alveococcosis leads to an increase in the number of radical resections, and modern surgical technologies and equipment can reduce the time of surgery, blood loss and the number of complications.
基本原理。肺泡球菌病是一种罕见的疾病，其诊断和治疗依赖于手术技术、设备和临床经验。的目标。目的:建立肝肺泡球菌病的诊断算法，比较不同时期肝肺泡球菌病的手术治疗结果。材料和方法。在第一阶段，我们对33例肺泡球菌病患者(对照组)进行了回顾性分析(1995-2007)。第二阶段，对39例患者(主要组)进行前瞻性临床研究(2008-2021)。根据纳入和排除标准确定患者人数，各研究组在年龄、性别、寄生虫定位等方面具有可比性(p < 0.05)。手术名称采用WHO肺泡球菌病分类。在主组中，酶免疫分析法的适用性增加;超声和计算机断层扫描;活组织检查。并发症发生率由对照组的54.6%降至主组的20.6%，降低了2.7倍(χ2 = 8.97;df = 1;p = 0.003)。对照组和主组的平均手术时间和平均出血量分别为:非典型切除- 220.4和180.2 min (p = 0.003)， 640.1和480.0 ml (p = 0.005);解剖切除- 296.2和247.2 min (p = 0.002)， 1450.2和1150.3 ml (p = 0.018);细胞减少切除术- 230.2和2000.1 min (p = 0.004)， 860.3和670.4 ml (p = 0.001)。对照组13例(39%)，主组3例(8%)，差异有统计学意义(χ2 = 4.74;df = 1;p = 0.029)。肺泡球菌病的及时诊断导致根治性切除的数量增加，现代手术技术和设备可以减少手术时间、出血量和并发症的数量。
{"title":"Diagnosis and comparative analysis of surgical treatment of patients with liver alveococcosis","authors":"V. Panteleev, M. Nartaylakov, I. Z. Salimgareev, A. Petrov","doi":"10.29413/abs.2023-8.2.21","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.21","url":null,"abstract":"Rationale. Alveococcosis is a rare disease, its diagnosis and treatment depend on surgical techniques, equipment and clinical experience. The aim. To develop a diagnostic algorithm and compare the results of surgical treatment of patients with liver alveococcosis in different periods of time.Materials and methods. At the first stage, we carried out a retrospective analysis (1995–2007) of 33 patients with alveococcosis (a comparison group). At the second stage, a prospective clinical study (2008–2021) was performed on 39 patients (the main group). The number of patients was determined in accordance with the inclusion and exclusion criteria, and the study groups were comparable in age, sex, parasite localization (p > 0.05). For the names of operations, the WHO classification of alveococcosis was used.Results. In the main group, there is an increase in the applicability of: enzyme immunoassay; ultrasound and computed tomography; biopsy. Complications decreased by 2.7 times from 54.6 % in the comparison group to 20.6 % in the main group (χ2 = 8.97; df = 1; p = 0.003). The average duration of operations, as well as the average volume of blood loss in the comparison group and the main group were, respectively: with atypical resection – 220.4 and 180.2 min (p = 0.003), 640.1 and 480.0 ml (p = 0.005); with anatomical resection – 296.2 and 247.2 min (p = 0.002), 1450.2 and 1150.3 ml (p = 0.018); with cytoreductive resection – 230.2 and 200.1 min (p = 0.004), 860.3 and 670.4 ml (p = 0.001). There were 13 (39 %) cytoreductive resections in the comparison group, and 3 (8 %) in the main group (χ2 = 4.74; df = 1; p = 0.029).Conclusion. Timely diagnosis of alveococcosis leads to an increase in the number of radical resections, and modern surgical technologies and equipment can reduce the time of surgery, blood loss and the number of complications.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75577946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-04DOI: 10.29413/abs.2023-8.2.16
J. Malisheva, T. Iureva, N. Volkova, J. V. Kursakova, S. Kolesnikov
The aim. To assess the dynamics of cytokine content in tear fluid of primary openangle glaucoma (POAG) patients at various terms after non-penetrating deep sclerectomy (NPDS) in relation to the functional state of the outflow tracts.Material and methods. We carried out prospective examination of 65 patients with advanced stage of primary open-angle glaucoma after NPDS. Depending on the course of the postoperative period and the conditions for achieving the hypotensive effect of NPDS, all patients were divided into three groups: group 1 – with the optimal hypotensive effect; group 2 – with the conditional hypotensive effect; group 3 – with no hypotensive effect after NPDS. The intraocular pressure and the concentration of TGF-β, MMP-9, IL-6, IL-8, VEGF-A (121 and 165) in the tear fluid were studied using ELISA method in pre- and postoperative period. We studied the outflow tracts using optical coherence tomography and carried out ultrastructural analysis of filtering blebs tissue.Results. In group 1, the minimum initial concentrations of IL-6, IL-8 and TGF-β in the tear fluid and their moderate increase in the postoperative period; high concentrations of MMP-9 at all stages and an increase in VEGF-A by 2 months after NPDS were registered. In group 2, there was a high level of VEGF-A in the tear fluid before and 2 months after NPDS, an increase in TGF-β, IL-6 and IL-8 in the tear fluid in the early period with their suppression in the late period, as well as an increase in MMP-9 in the early postoperative period. Group 3 had maximum concentrations of TGF-β and IL-8 in the tear fluid initially and in the early postoperative period, suppression of MMP-9 in the tear fluid 2 weeks after and of VEGF-A 2 months after NPDS.Conclusion. Initially high concentrations of IL-6, IL-8, and TGF-β in the tear fluid and the suppression of MMP-9 and VEGF-A in the postoperative period contribute to the surgical failure of the NPDS.
{"title":"Prospective assessment of cytokines and regulatory proteins concentration in the tear fluid of POAG patients with various hypotensive effects after non-penetrating deep sclerectomy","authors":"J. Malisheva, T. Iureva, N. Volkova, J. V. Kursakova, S. Kolesnikov","doi":"10.29413/abs.2023-8.2.16","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.16","url":null,"abstract":"The aim. To assess the dynamics of cytokine content in tear fluid of primary openangle glaucoma (POAG) patients at various terms after non-penetrating deep sclerectomy (NPDS) in relation to the functional state of the outflow tracts.Material and methods. We carried out prospective examination of 65 patients with advanced stage of primary open-angle glaucoma after NPDS. Depending on the course of the postoperative period and the conditions for achieving the hypotensive effect of NPDS, all patients were divided into three groups: group 1 – with the optimal hypotensive effect; group 2 – with the conditional hypotensive effect; group 3 – with no hypotensive effect after NPDS. The intraocular pressure and the concentration of TGF-β, MMP-9, IL-6, IL-8, VEGF-A (121 and 165) in the tear fluid were studied using ELISA method in pre- and postoperative period. We studied the outflow tracts using optical coherence tomography and carried out ultrastructural analysis of filtering blebs tissue.Results. In group 1, the minimum initial concentrations of IL-6, IL-8 and TGF-β in the tear fluid and their moderate increase in the postoperative period; high concentrations of MMP-9 at all stages and an increase in VEGF-A by 2 months after NPDS were registered. In group 2, there was a high level of VEGF-A in the tear fluid before and 2 months after NPDS, an increase in TGF-β, IL-6 and IL-8 in the tear fluid in the early period with their suppression in the late period, as well as an increase in MMP-9 in the early postoperative period. Group 3 had maximum concentrations of TGF-β and IL-8 in the tear fluid initially and in the early postoperative period, suppression of MMP-9 in the tear fluid 2 weeks after and of VEGF-A 2 months after NPDS.Conclusion. Initially high concentrations of IL-6, IL-8, and TGF-β in the tear fluid and the suppression of MMP-9 and VEGF-A in the postoperative period contribute to the surgical failure of the NPDS.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89725312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-03DOI: 10.29413/abs.2023-8.2.11
A. Akhova, A. Tkachenko
Background. Some stress responses contribute to the formation of bacterial antibiotic resistance, including the soxRS oxidative defense regulon. Elevation of reactive oxygen species production and oxidative stress was detected in bacterial cells exposed to various environmental stresses. It can be supposed that a stress-mediated increase in the level of reactive oxygen species will activate the expression of the soxRS regulon genes, which may provide pre-adaptation to antibiotics.The aim. To study changes in the expression of soxRS regulon genes in Escherichia coli cells exposed to NaCl, acetic acid, and heating.Materials and methods. Gene expression was measured in cells bearing reporter gene fusions (soxS::lacZ, nfo::lacZ). An overnight broth culture was diluted in fresh LB broth to OD600 = 0.1 and cultivated at 37 °C without stirring until OD600 = 0.3, then the stressors were applied.Results. Exposure to NaCl and acetic acid activated the expression of soxRS regulon genes, while heating caused a decrease in gene expression. An increase in the expression level was observed in cells subjected to stresses of low intensity (which did not cause a decrease in the number of colony-forming units (CFU) by the 4th hour of exposure compared to the beginning of the stress exposure) and medium intensity (which caused a 10-fold decrease in the number of CFU), whereas high-intensity stresses (which caused a decrease in the number of CFU by more than 10 times), regardless of their nature, were accompanied by a decrease in the expression of the soxRS regulon genes.Conclusion. Under the conditions studied, only the osmotic stress caused by the addition of NaCl was accompanied by a significant activation of the soxRS regulon genes. Sublethal exposure to NaCl, causing an increase in the expression of soxRS regulon genes by 2–2.5 times, may provide pre-adaptation of bacteria to the factors that this regulon is aimed at counteracting, including antibacterial drugs.
{"title":"Expression of the soxRS regulon in bacterial cells exposed to various stress factors","authors":"A. Akhova, A. Tkachenko","doi":"10.29413/abs.2023-8.2.11","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.11","url":null,"abstract":"Background. Some stress responses contribute to the formation of bacterial antibiotic resistance, including the soxRS oxidative defense regulon. Elevation of reactive oxygen species production and oxidative stress was detected in bacterial cells exposed to various environmental stresses. It can be supposed that a stress-mediated increase in the level of reactive oxygen species will activate the expression of the soxRS regulon genes, which may provide pre-adaptation to antibiotics.The aim. To study changes in the expression of soxRS regulon genes in Escherichia coli cells exposed to NaCl, acetic acid, and heating.Materials and methods. Gene expression was measured in cells bearing reporter gene fusions (soxS::lacZ, nfo::lacZ). An overnight broth culture was diluted in fresh LB broth to OD600 = 0.1 and cultivated at 37 °C without stirring until OD600 = 0.3, then the stressors were applied.Results. Exposure to NaCl and acetic acid activated the expression of soxRS regulon genes, while heating caused a decrease in gene expression. An increase in the expression level was observed in cells subjected to stresses of low intensity (which did not cause a decrease in the number of colony-forming units (CFU) by the 4th hour of exposure compared to the beginning of the stress exposure) and medium intensity (which caused a 10-fold decrease in the number of CFU), whereas high-intensity stresses (which caused a decrease in the number of CFU by more than 10 times), regardless of their nature, were accompanied by a decrease in the expression of the soxRS regulon genes.Conclusion. Under the conditions studied, only the osmotic stress caused by the addition of NaCl was accompanied by a significant activation of the soxRS regulon genes. Sublethal exposure to NaCl, causing an increase in the expression of soxRS regulon genes by 2–2.5 times, may provide pre-adaptation of bacteria to the factors that this regulon is aimed at counteracting, including antibacterial drugs.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75670836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. MicroRNAs are short (20–22 nucleotides) non-coding RNAs that can posttranscriptionally regulate gene expression and are considered a regulator of the innate immunity system. Previously, many papers were published on the prediction of the interaction of the single-stranded (+)RNA virus SARS-CoV-2 with human microRNAs, as well as on the profile of circulating microRNAs in patients with COVID-19 of varying severity. However, no works are analyzing the possible contribution of miRNAs circulating in blood plasma to the severity of COVID-19.The aim. To study the features of the blood plasma microRNA profile of patients with different severity of the new coronavirus infection COVID-19 and to evaluate the possibility of microRNA interaction with the SARS-CoV-2 genome.Materials and methods. The results of NGS sequencing of plasma miRNAs of 3 recovered and 8 deceased patients with a highly severe form of COVID-19 were studied. Differentially presented microRNAs were determined using bioinformatics methods, and their binding sites with the SARS-CoV-2 genome were predicted.Results. This study demonstrates that in patients who have recovered from a highly severe form of COVID-19, the level of hsa-miR-19b-3p in the blood plasma is significantly increased. This microRNA makes up about 1.5 % of all circulating microRNAs and can bind to SARS-CoV-2 regions encoding proteins that suppress intracellular immunity mechanisms (NSP3, NSP9). In addition, this miRNA can stimulate the functional activity and proliferation of cytotoxic T-lymphocytes, one of the critical components of acquired cellular immunity against SARS-CoV-2.Conclusion. The results of the study can be used in the development of antiviral drugs based on RNA interference, as well as in the development of predictive test systems to optimize the tactics of treating patients with COVID-19.
{"title":"The role of circulating miR-19b miRNA in predicting the outcome of COVID-19","authors":"M. Shkurnikov, S. Kolesnikov","doi":"10.29413/abs.2023-8.2.3","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.3","url":null,"abstract":"Background. MicroRNAs are short (20–22 nucleotides) non-coding RNAs that can posttranscriptionally regulate gene expression and are considered a regulator of the innate immunity system. Previously, many papers were published on the prediction of the interaction of the single-stranded (+)RNA virus SARS-CoV-2 with human microRNAs, as well as on the profile of circulating microRNAs in patients with COVID-19 of varying severity. However, no works are analyzing the possible contribution of miRNAs circulating in blood plasma to the severity of COVID-19.The aim. To study the features of the blood plasma microRNA profile of patients with different severity of the new coronavirus infection COVID-19 and to evaluate the possibility of microRNA interaction with the SARS-CoV-2 genome.Materials and methods. The results of NGS sequencing of plasma miRNAs of 3 recovered and 8 deceased patients with a highly severe form of COVID-19 were studied. Differentially presented microRNAs were determined using bioinformatics methods, and their binding sites with the SARS-CoV-2 genome were predicted.Results. This study demonstrates that in patients who have recovered from a highly severe form of COVID-19, the level of hsa-miR-19b-3p in the blood plasma is significantly increased. This microRNA makes up about 1.5 % of all circulating microRNAs and can bind to SARS-CoV-2 regions encoding proteins that suppress intracellular immunity mechanisms (NSP3, NSP9). In addition, this miRNA can stimulate the functional activity and proliferation of cytotoxic T-lymphocytes, one of the critical components of acquired cellular immunity against SARS-CoV-2.Conclusion. The results of the study can be used in the development of antiviral drugs based on RNA interference, as well as in the development of predictive test systems to optimize the tactics of treating patients with COVID-19.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75585871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A systematic review and meta-analysis of data on the problem of enhanced recovery in urology was performed. Inclusion criteria – clinical trials of the enhanced recovery program in urology.The aim of the study was to assess the significance of the enhanced recovery program (ERP) in the provision of surgical care in the “urology” profile.Materials and methods. The systematic review was performed according to the guidelines for the presentation of systematic reviews and meta-analyses by PRISMA. The registration number in the international system Prospero was received (CRD42022358982). The review included 364 studies. Studies in urologic oncology were excluded from the meta-analysis. The meta-analysis included 15 studies involving 2293 subjects. A comparison was made between the application of ERP and the standard treatment protocol.Results. The use of ERP leads to an expected two-fold reduction in the duration of postoperative length of hospitalization (OR = –1.96; 95% CI: –2.56÷–1.36; p < 0.00001). The reduction in the duration of hospitalization with the use of ERP in urology does not lead to the increased risk of readmission or re-operation (p = 0.35). The risks of developing postoperative complications ≥ Class 2 by Clavien – Dindo classification were comparable in both groups (p = 0.13). The use of ERP increases the expected success of the treatment by 1.74 times (OR = 1.74; 95% CI: 1.08–2.79; p = 0.02). With the use of ERP in reconstructive urology, a significantly lower risk of complications was established (p = 0.02).Conclusion. The ERP program allows you to reduce the time and cost of treatment, reduce the likelihood of re-hospitalization and achieve better treatment results. The use of ERP is not accompanied by increased risk of complications > Class 2.
{"title":"The enhanced recovery program in urology. Systematic review and meta-analysis","authors":"V. Vorobev, V. Beloborodov, A. Tukhiev","doi":"10.29413/abs.2023-8.2.7","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.7","url":null,"abstract":"A systematic review and meta-analysis of data on the problem of enhanced recovery in urology was performed. Inclusion criteria – clinical trials of the enhanced recovery program in urology.The aim of the study was to assess the significance of the enhanced recovery program (ERP) in the provision of surgical care in the “urology” profile.Materials and methods. The systematic review was performed according to the guidelines for the presentation of systematic reviews and meta-analyses by PRISMA. The registration number in the international system Prospero was received (CRD42022358982). The review included 364 studies. Studies in urologic oncology were excluded from the meta-analysis. The meta-analysis included 15 studies involving 2293 subjects. A comparison was made between the application of ERP and the standard treatment protocol.Results. The use of ERP leads to an expected two-fold reduction in the duration of postoperative length of hospitalization (OR = –1.96; 95% CI: –2.56÷–1.36; p < 0.00001). The reduction in the duration of hospitalization with the use of ERP in urology does not lead to the increased risk of readmission or re-operation (p = 0.35). The risks of developing postoperative complications ≥ Class 2 by Clavien – Dindo classification were comparable in both groups (p = 0.13). The use of ERP increases the expected success of the treatment by 1.74 times (OR = 1.74; 95% CI: 1.08–2.79; p = 0.02). With the use of ERP in reconstructive urology, a significantly lower risk of complications was established (p = 0.02).Conclusion. The ERP program allows you to reduce the time and cost of treatment, reduce the likelihood of re-hospitalization and achieve better treatment results. The use of ERP is not accompanied by increased risk of complications > Class 2.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89006053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Semenova, S. Kolesnikov, E. V. Vyrupaeva, L. Sholokhov, L. V. Rychkova, А. G. Petrova, M. R. Akhmedzyanova, M. Darenskaya, L. Kolesnikova
The aim. To assess the thyroid status and its relationship with tumor necrosis factor alpha (TNF-alpha) in post-reproductive women in the acute phase of the COVID-19 of moderate course and 12 months after the disease.Materials and methods. 85 women aged 45 to 69 years were divided into groups: women without COVID-19, not vaccinated, with no antibodies to COVID-19 (IgG) – control group (n = 15); women in the acute phase of COVID-19 of a moderate course, accompanied by pneumonia – main group (n = 57); patients from the main group who agreed to be examined 12 months after having COVID-19 (n = 14); women with IgG in blood who deny any symptoms of COVID-19 in the last 12 months – asymptomatic COVID-19 (n = 13). Using hormone replacement therapy and the presence of thyroid disease in history were the exclusion criteria from the study.Results. 75.4 % of patients with COVID-19 had euthyroidism, 12.3 % had subclinical hyperthyroidism. An increase of free thyroxine (free T4) level in women with COVID-19 as compared to the control group (p = 0.004) and the group with asymptomatic COVID-19 (p = 0.054) was found. There was no statistically significant difference in the level of thyroid stimulating hormone between the groups. The level of C-reactive protein in women with COVID-19 was naturally higher as compared to the control group (p = 0.009) and the group of asymptomatic patients (p = 0.001). A lower TNF-alpha level was found in the group of patients without clinical signs of COVID-19 as compared to the control group (p = 0.007) and the group with COVID-19 (p = 0.00007). The analysis of correlation relationships revealed a positive correlation between of free T4 and TNF-alpha levels in women with COVID-19 (r = 0.38, p = 0.004).Conclusions. The moderate course of COVID-19 in the post-reproductive women is associated with an increase of free T4 level, which positively correlates with TNF-alpha level. Twelve months after COVID-19, thyroid status in women remains at the level of the acute phase of the disease.
{"title":"Thyroid status and TNF-alpha in post-reproductive women with COVID-19 and 12 months after the disease","authors":"N. Semenova, S. Kolesnikov, E. V. Vyrupaeva, L. Sholokhov, L. V. Rychkova, А. G. Petrova, M. R. Akhmedzyanova, M. Darenskaya, L. Kolesnikova","doi":"10.29413/abs.2023-8.2.4","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.4","url":null,"abstract":"The aim. To assess the thyroid status and its relationship with tumor necrosis factor alpha (TNF-alpha) in post-reproductive women in the acute phase of the COVID-19 of moderate course and 12 months after the disease.Materials and methods. 85 women aged 45 to 69 years were divided into groups: women without COVID-19, not vaccinated, with no antibodies to COVID-19 (IgG) – control group (n = 15); women in the acute phase of COVID-19 of a moderate course, accompanied by pneumonia – main group (n = 57); patients from the main group who agreed to be examined 12 months after having COVID-19 (n = 14); women with IgG in blood who deny any symptoms of COVID-19 in the last 12 months – asymptomatic COVID-19 (n = 13). Using hormone replacement therapy and the presence of thyroid disease in history were the exclusion criteria from the study.Results. 75.4 % of patients with COVID-19 had euthyroidism, 12.3 % had subclinical hyperthyroidism. An increase of free thyroxine (free T4) level in women with COVID-19 as compared to the control group (p = 0.004) and the group with asymptomatic COVID-19 (p = 0.054) was found. There was no statistically significant difference in the level of thyroid stimulating hormone between the groups. The level of C-reactive protein in women with COVID-19 was naturally higher as compared to the control group (p = 0.009) and the group of asymptomatic patients (p = 0.001). A lower TNF-alpha level was found in the group of patients without clinical signs of COVID-19 as compared to the control group (p = 0.007) and the group with COVID-19 (p = 0.00007). The analysis of correlation relationships revealed a positive correlation between of free T4 and TNF-alpha levels in women with COVID-19 (r = 0.38, p = 0.004).Conclusions. The moderate course of COVID-19 in the post-reproductive women is associated with an increase of free T4 level, which positively correlates with TNF-alpha level. Twelve months after COVID-19, thyroid status in women remains at the level of the acute phase of the disease.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80592293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-03DOI: 10.29413/abs.2023-8.2.10
S. V. Kolomencev, S. N. Yanishevskiy, I. A. Voznjouk, N. V. Tsygan, I. V. Litvinenko, E. I. Shermatyuk, O. M. Ilyina, Е. A. Kurnikova, T. V. Sergeeva
Perioperative ischemic stroke is a potentially fatal complication that greatly increases the risk of poor outcome in surgical patients. Despite the relatively low prevalence among patients undergoing non-cardiosurgical and non-neurosurgical interventions (about 0.1–1.0 %), the total number of annually developing perioperative ischemic strokes in patients of this profile is high due to the large number of operations performed in the world. Since the publication in 2014 of the last fundamental work on the prevention of perioperative stroke, approaches to primary and secondary prevention, diagnosis, conservative and reperfusion treatment of ischemic stroke have been seriously modified. The numerous changes that have taken place have created the prerequisites for revising existing approaches to providing care for perioperative ischemic stroke. In 2021, updated documents of foreign researchers/ associations on the problem of perioperative ischemic stroke in non-cardiac and nonneurosurgical patients were published. This review, which consists of two parts, presents current data that summarizes the most relevant information on this topic. The first part of the review outlines the general provisions on perioperative ischemic stroke (definition, risk factors, pathogenesis, predictive models), strategies for pre- and intraoperative prevention.
{"title":"Prevention of perioperative ischemic stroke after non-cardiac and non-neurosurgical operations in the light of the Scientific Statement and Guidelines for the Secondary Prevention of Ischemic Stroke and Transient Ischemic Attack AHA/ASA 2021 Part 1: Definition, risk factors, pathogenesis, prognosis, principles of pre- and intraoperative prevention","authors":"S. V. Kolomencev, S. N. Yanishevskiy, I. A. Voznjouk, N. V. Tsygan, I. V. Litvinenko, E. I. Shermatyuk, O. M. Ilyina, Е. A. Kurnikova, T. V. Sergeeva","doi":"10.29413/abs.2023-8.2.10","DOIUrl":"https://doi.org/10.29413/abs.2023-8.2.10","url":null,"abstract":"Perioperative ischemic stroke is a potentially fatal complication that greatly increases the risk of poor outcome in surgical patients. Despite the relatively low prevalence among patients undergoing non-cardiosurgical and non-neurosurgical interventions (about 0.1–1.0 %), the total number of annually developing perioperative ischemic strokes in patients of this profile is high due to the large number of operations performed in the world. Since the publication in 2014 of the last fundamental work on the prevention of perioperative stroke, approaches to primary and secondary prevention, diagnosis, conservative and reperfusion treatment of ischemic stroke have been seriously modified. The numerous changes that have taken place have created the prerequisites for revising existing approaches to providing care for perioperative ischemic stroke. In 2021, updated documents of foreign researchers/ associations on the problem of perioperative ischemic stroke in non-cardiac and nonneurosurgical patients were published. This review, which consists of two parts, presents current data that summarizes the most relevant information on this topic. The first part of the review outlines the general provisions on perioperative ischemic stroke (definition, risk factors, pathogenesis, predictive models), strategies for pre- and intraoperative prevention.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135111150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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