Pub Date : 2023-10-04DOI: 10.29413/abs.2023-8.4.18
T. N. Iureva, O. V. Pisarevskaya
The aim . To present a clinical case of a patient with corneal edema caused by undiagnosed iridocorneal endothelial syndrome after SMILE surgery. Iridocorneal endothelial syndrome is characterized by abnormal proliferation of the corneal endothelium beyond the Schwalbe line, blockade of the iridocorneal angle, specific changes in the shape of the pupil and the formation of synechiae between adjacent structures of the anterior chamber angle of the eye, which is accompanied by the development of pretrabecular retention of intraocular aqueous humour, increased intraocular pressure (IOP) and subsequent development of glaucoma in 46–82 % of patients. One of the forms of the disease is Chandler’s syndrome, which clinically in the initial stages may be accompanied by the formation of only moderate ectropion. At the same time, endothelial dysfunction is characterized by a significant decrease in the number of cells, a change in their shape and the appearance of epithelioid cells with a hyper-reflective nucleus (the so-called “ICE (iridocorneal endothelial) cells”), with a violation of their natural pumping function. The article presents a clinical case of a patient who underwent a standard examination and symptomatic treatment after laser keratorefractive surgery for myopia correction. Low visual acuity, diffuse corneal edema, IOP decompensation of unclear genesis were observed in one operated eye. Conclusion . Keratorefractive operations can act as a trigger that stimulates the transition of the latent form into a clinically developed pathological process, which causes a decrease in corrected visual acuity due to the formation of corneal edema and glaucoma optic neuropathy.
{"title":"Corneal edema caused by undiagnosed iridocorneal endothelial syndrome after SMILE","authors":"T. N. Iureva, O. V. Pisarevskaya","doi":"10.29413/abs.2023-8.4.18","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.18","url":null,"abstract":"The aim . To present a clinical case of a patient with corneal edema caused by undiagnosed iridocorneal endothelial syndrome after SMILE surgery. Iridocorneal endothelial syndrome is characterized by abnormal proliferation of the corneal endothelium beyond the Schwalbe line, blockade of the iridocorneal angle, specific changes in the shape of the pupil and the formation of synechiae between adjacent structures of the anterior chamber angle of the eye, which is accompanied by the development of pretrabecular retention of intraocular aqueous humour, increased intraocular pressure (IOP) and subsequent development of glaucoma in 46–82 % of patients. One of the forms of the disease is Chandler’s syndrome, which clinically in the initial stages may be accompanied by the formation of only moderate ectropion. At the same time, endothelial dysfunction is characterized by a significant decrease in the number of cells, a change in their shape and the appearance of epithelioid cells with a hyper-reflective nucleus (the so-called “ICE (iridocorneal endothelial) cells”), with a violation of their natural pumping function. The article presents a clinical case of a patient who underwent a standard examination and symptomatic treatment after laser keratorefractive surgery for myopia correction. Low visual acuity, diffuse corneal edema, IOP decompensation of unclear genesis were observed in one operated eye. Conclusion . Keratorefractive operations can act as a trigger that stimulates the transition of the latent form into a clinically developed pathological process, which causes a decrease in corrected visual acuity due to the formation of corneal edema and glaucoma optic neuropathy.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135645490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-04DOI: 10.29413/abs.2023-8.4.19
I. V. Lev, N. M. Agarkov, A. E. Kopylov
Background . The development of diabetic retinopathy is favoured by immunological factors such as interleukins (IL) and chemokines. However, analysis of blood interleukins in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus, who have biological age acceleration, has not yet been presented in publications. The aim of the research . To study the content of blood interleukins in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus, who have an excess of biological age over chronological age. Materials and methods . 241 patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus were examined in a clinical setting. Biological age acceleration over chronological age was found in 148 patients, biological and chronological age concorded in 51 patients. The content of interleukins in the blood was studied in all patients using an enzyme-linked immunoassay. Results . The concentration of blood interleukins in patients with biological age exceeding chronological, compared with patients aged 45–59 years with concordance of biological and chronological age, was statistically significantly different for most blood interleukins and especially for IL-6, the concentration of which was 20.8 ± 1,2 pg/ml versus 3.9 ± 0.6 pg/ml, respectively (p < 0.001). IL-13, IL-17 were significantly increased among patients with biological age acceleration over chronological; their concentrations were 2.1 ± 0.4 and 16.5 ± 0.6 pg/ml versus 0.5 ± 0.2 and 7.9 ± 0.7 pg/ml in the comparison group (p < 0.001). In contrast, IL-4 and IL-10 levels were higher in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus and with concordance of biological and chronological age. Conclusion . IL-6, IL-8, IL-13, IL-17, IL-4 and IL-10 may serve as markers of biological age dissociation in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus.
{"title":"Dissociation of biological age and blood interleukins in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus","authors":"I. V. Lev, N. M. Agarkov, A. E. Kopylov","doi":"10.29413/abs.2023-8.4.19","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.19","url":null,"abstract":"Background . The development of diabetic retinopathy is favoured by immunological factors such as interleukins (IL) and chemokines. However, analysis of blood interleukins in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus, who have biological age acceleration, has not yet been presented in publications. The aim of the research . To study the content of blood interleukins in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus, who have an excess of biological age over chronological age. Materials and methods . 241 patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus were examined in a clinical setting. Biological age acceleration over chronological age was found in 148 patients, biological and chronological age concorded in 51 patients. The content of interleukins in the blood was studied in all patients using an enzyme-linked immunoassay. Results . The concentration of blood interleukins in patients with biological age exceeding chronological, compared with patients aged 45–59 years with concordance of biological and chronological age, was statistically significantly different for most blood interleukins and especially for IL-6, the concentration of which was 20.8 ± 1,2 pg/ml versus 3.9 ± 0.6 pg/ml, respectively (p < 0.001). IL-13, IL-17 were significantly increased among patients with biological age acceleration over chronological; their concentrations were 2.1 ± 0.4 and 16.5 ± 0.6 pg/ml versus 0.5 ± 0.2 and 7.9 ± 0.7 pg/ml in the comparison group (p < 0.001). In contrast, IL-4 and IL-10 levels were higher in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus and with concordance of biological and chronological age. Conclusion . IL-6, IL-8, IL-13, IL-17, IL-4 and IL-10 may serve as markers of biological age dissociation in patients aged 45–59 years with diabetic retinopathy in type 2 diabetes mellitus.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135645491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. K. Doroshchenko, O. V. Lisak, O. V. Suntsova, Ju. S. Savinova, I. V. Kozlova
The ixodid tick Haemaphysalis concinna (Koch, 1844) is a carrier of pathogens of vector-borne diseases of viral, bacterial and protozoal etiology. This tick was previously considered an adventive relict species in the Baikal region, but has recently shown a tendency to increase its numbers and expand its habitat. The aim of the study . To generalize the available and newly received information on the distribution of the H. concinna tick in the Irkutsk region and the Republic of Buryatia; to carry out the comparative analysis of the results in order to identify the dynamics of the development of populations of this species of ixodid ticks in the Baikal region. Materials and methods . The coordinates of H. concinna detection points in our studies were obtained using a GPS navigator directly in the field. Ticks were caught during the period of their maximum activity from plants using a flannel flag. Approximate geographic coordinates of H. concinna tick detection points were established when analyzing the maps published by other researchers. Results . As a result of the generalization of our own data and data from literary sources, 52 georeferenced detection points of H. concinna were obtained on the territory of the Baikal region. A map showing the distribution of H. concinna in the territory of the Irkutsk region and the Republic of Buryatia is presented. It is shown that in a number of surveyed areas there are stable populations of this species of tick, which tend to increase in their numbers and expand their range. Conclusions . Considering these data, as well as the fact that H. concinna ticks are actively involved in the circulation of pathogens of natural focal diseases in humans and animals, we can conclude that it is necessary to systematically monitor the populations of this vector species in the Baikal region.
{"title":"Data on the distribution of the <i>Haemaphysalis concinna</i> tick in the Irkutsk region and the Republic of Buryatia","authors":"E. K. Doroshchenko, O. V. Lisak, O. V. Suntsova, Ju. S. Savinova, I. V. Kozlova","doi":"10.29413/abs.2023-8.4.9","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.9","url":null,"abstract":"The ixodid tick Haemaphysalis concinna (Koch, 1844) is a carrier of pathogens of vector-borne diseases of viral, bacterial and protozoal etiology. This tick was previously considered an adventive relict species in the Baikal region, but has recently shown a tendency to increase its numbers and expand its habitat. The aim of the study . To generalize the available and newly received information on the distribution of the H. concinna tick in the Irkutsk region and the Republic of Buryatia; to carry out the comparative analysis of the results in order to identify the dynamics of the development of populations of this species of ixodid ticks in the Baikal region. Materials and methods . The coordinates of H. concinna detection points in our studies were obtained using a GPS navigator directly in the field. Ticks were caught during the period of their maximum activity from plants using a flannel flag. Approximate geographic coordinates of H. concinna tick detection points were established when analyzing the maps published by other researchers. Results . As a result of the generalization of our own data and data from literary sources, 52 georeferenced detection points of H. concinna were obtained on the territory of the Baikal region. A map showing the distribution of H. concinna in the territory of the Irkutsk region and the Republic of Buryatia is presented. It is shown that in a number of surveyed areas there are stable populations of this species of tick, which tend to increase in their numbers and expand their range. Conclusions . Considering these data, as well as the fact that H. concinna ticks are actively involved in the circulation of pathogens of natural focal diseases in humans and animals, we can conclude that it is necessary to systematically monitor the populations of this vector species in the Baikal region.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.29413/abs.2023-8.4.14
A. N. Sumin, A. V. Shcheglova, M. I. Anichkova, D. N. Fedorova, K. A. Shabalina
Recently, it has become common to identify type D personality, which is predisposed to the development of psychological distress. Negative behavioral characteristics of individuals with type D personality contribute not only to the development of cardiovascular diseases, but also to other comorbid pathologies that can influence the progression and prognosis of coronary heart disease. The aim of the study . To identify clinical and psychological correlations with type D personality in patients with chronic coronary syndrome. Methods . The study included 113 patients (68 men and 45 women; median age – 64 years) admitted for planned percutaneous coronary intervention at the Research Institute for Complex Issues of Cardiovascular Diseases (Kemerovo, Russian Federation). Based on the results of the DS-14 test, patients were divided into two groups: patients with type D personality (n = 40) and patients without this type (n = 73). Results . In patients with chronic coronary syndrome with type D personality, compared with patients without this type, concomitant diabetes mellitus (35 % and 15 %, respectively; p = 0.018), signs of diastolic dysfunction of left (Е/е’ ratio 7.1 [6.48; 8.0] and 5.0 [4.55; 5.74], respectively; p = 0.0038) and right (Et/At ratio – 0.8 [0.66; 1.35] and 1.38 [1.28; 1.63], respectively; p = 0.014) ventricles were more often diagnosed. Correlation analysis revealed associations of diabetes mellitus with type D personality (r = 0.243; p = 0.011), severity of negative excitability (r = 0.253; p = 0.008) and social suppression (r = 0.224; p = 0.020), as well as association of ankle-brachial index (ABI) with the severity of negative excitability (r = 0.393; p = 0.004) and social suppression (r = 0.414; p = 0.002). Conclusion . In patients having chronic coronary syndrome with type D personality, concomitant diabetes mellitus, as well as left and right ventricular filling disorders are more often detected. Correlation analysis revealed associations of diabetes mellitus with type D personality and its subscales; the ABI level was associated with subscales of type D personality, but not with the level of anxiety and depression.
{"title":"Clinical and psychological correlations with type D personality in patients with chronic coronary syndrome","authors":"A. N. Sumin, A. V. Shcheglova, M. I. Anichkova, D. N. Fedorova, K. A. Shabalina","doi":"10.29413/abs.2023-8.4.14","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.14","url":null,"abstract":"Recently, it has become common to identify type D personality, which is predisposed to the development of psychological distress. Negative behavioral characteristics of individuals with type D personality contribute not only to the development of cardiovascular diseases, but also to other comorbid pathologies that can influence the progression and prognosis of coronary heart disease. The aim of the study . To identify clinical and psychological correlations with type D personality in patients with chronic coronary syndrome. Methods . The study included 113 patients (68 men and 45 women; median age – 64 years) admitted for planned percutaneous coronary intervention at the Research Institute for Complex Issues of Cardiovascular Diseases (Kemerovo, Russian Federation). Based on the results of the DS-14 test, patients were divided into two groups: patients with type D personality (n = 40) and patients without this type (n = 73). Results . In patients with chronic coronary syndrome with type D personality, compared with patients without this type, concomitant diabetes mellitus (35 % and 15 %, respectively; p = 0.018), signs of diastolic dysfunction of left (Е/е’ ratio 7.1 [6.48; 8.0] and 5.0 [4.55; 5.74], respectively; p = 0.0038) and right (Et/At ratio – 0.8 [0.66; 1.35] and 1.38 [1.28; 1.63], respectively; p = 0.014) ventricles were more often diagnosed. Correlation analysis revealed associations of diabetes mellitus with type D personality (r = 0.243; p = 0.011), severity of negative excitability (r = 0.253; p = 0.008) and social suppression (r = 0.224; p = 0.020), as well as association of ankle-brachial index (ABI) with the severity of negative excitability (r = 0.393; p = 0.004) and social suppression (r = 0.414; p = 0.002). Conclusion . In patients having chronic coronary syndrome with type D personality, concomitant diabetes mellitus, as well as left and right ventricular filling disorders are more often detected. Correlation analysis revealed associations of diabetes mellitus with type D personality and its subscales; the ABI level was associated with subscales of type D personality, but not with the level of anxiety and depression.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. A. Kulikov, N. V. Nizyaeva, T. V. Sukhacheva, R. A. Serov, N. B. Tikhonova, T. V. Fokina, A. P. Milovanov, T. N. Belousova, E. R. Milyutina, L. M. Mikhaleva
The aim . To carry out a comparative morphological characteristic of the uteroplacental area with abnormal placentation – pl. accreta, pl. increta, pl. percreta. Materials and methods . The study included 47 patients with atypical placentation; the comparison group included 10 healthy pregnant women with uterine scar after a previous caesarean section. A histological study of uteroplacental area samples was performed with hematoxylin and eosin, methylene blue staining. An immunohistochemical study with primary antibodies to cytokeratin 7 (CK7), Hif2a, vascular endothelial growth factor, α-SMA was carried out. The differences between the compared values were considered to be statistically significant at p < 0.05. The results of the study . Pl. accreta was determined in 12 (25.5 %), pl. increta – in 30 (63.9 %), pl. percreta – in 5 (10.6 %) patients. In all patients of the main group, the decidua was completely or partially absent in the area of abnormal placentation or was replaced by an uneven layer of fetal fibrinoid. Cases when placental villi unevenly penetrated into the thickness of myometrium in the form of “tongues” or “coves” bordered by fetal fibrinoid and often located intermuscularly were defined as pl. increta (n = 26). Cases with the placental villi ingrowth to the serous membrane were considered as pl. percreta (n = 5). In cases with deep variants of ingrowth (pl. increta and pl. percreta) (n = 31), the villi were visualized in the lumen of the vessels and the thinning of the lower uterine segment with the presence of stretched muscle bundles was revealed. Aseptic necrosis of the myometrium was found: in 2 (16.7 %) of 12 women with pl. accreta, in 26 (86.7 %) of 30 women with pl. increta and in 5 (100 %) women with pl. percreta. There were no areas of necrosis in the myometrium of the women of comparison group. Conclusion . The appearance and increase of myometrial necrosis zones in response to an increase in the depth of placental villus ingrowth were detected. Myometrial necrosis zones could be the cause of activation of angiogenic factors and an important stimulus for the development of abnormal vascularization in placenta accreta spectrum.
{"title":"Comparative morphological characteristics of the uteroplacental area in abnormal placentation","authors":"I. A. Kulikov, N. V. Nizyaeva, T. V. Sukhacheva, R. A. Serov, N. B. Tikhonova, T. V. Fokina, A. P. Milovanov, T. N. Belousova, E. R. Milyutina, L. M. Mikhaleva","doi":"10.29413/abs.2023-8.4.8","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.8","url":null,"abstract":"The aim . To carry out a comparative morphological characteristic of the uteroplacental area with abnormal placentation – pl. accreta, pl. increta, pl. percreta. Materials and methods . The study included 47 patients with atypical placentation; the comparison group included 10 healthy pregnant women with uterine scar after a previous caesarean section. A histological study of uteroplacental area samples was performed with hematoxylin and eosin, methylene blue staining. An immunohistochemical study with primary antibodies to cytokeratin 7 (CK7), Hif2a, vascular endothelial growth factor, α-SMA was carried out. The differences between the compared values were considered to be statistically significant at p < 0.05. The results of the study . Pl. accreta was determined in 12 (25.5 %), pl. increta – in 30 (63.9 %), pl. percreta – in 5 (10.6 %) patients. In all patients of the main group, the decidua was completely or partially absent in the area of abnormal placentation or was replaced by an uneven layer of fetal fibrinoid. Cases when placental villi unevenly penetrated into the thickness of myometrium in the form of “tongues” or “coves” bordered by fetal fibrinoid and often located intermuscularly were defined as pl. increta (n = 26). Cases with the placental villi ingrowth to the serous membrane were considered as pl. percreta (n = 5). In cases with deep variants of ingrowth (pl. increta and pl. percreta) (n = 31), the villi were visualized in the lumen of the vessels and the thinning of the lower uterine segment with the presence of stretched muscle bundles was revealed. Aseptic necrosis of the myometrium was found: in 2 (16.7 %) of 12 women with pl. accreta, in 26 (86.7 %) of 30 women with pl. increta and in 5 (100 %) women with pl. percreta. There were no areas of necrosis in the myometrium of the women of comparison group. Conclusion . The appearance and increase of myometrial necrosis zones in response to an increase in the depth of placental villus ingrowth were detected. Myometrial necrosis zones could be the cause of activation of angiogenic factors and an important stimulus for the development of abnormal vascularization in placenta accreta spectrum.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polycystic ovary syndrome (PCOS) is considered a common endocrine disorder among women of reproductive age, and the associated health risks persist throughout life. At the same time, there is a wide range of variations in the incidence of the syndrome (4–21 %), which in turn is explained by the influence of the study population characteristics, including ethnicity and race, as well as the applied diagnostic criteria. The aim of the study . To systematize the available data on the prevalence of polycystic ovary syndrome using the Rotterdam 2003 criteria in a population of women of reproductive age. Materials and methods . The search for information was carried out using Internet resources (PubMed, EMBASE, Google Scholar, eLibrary). Literature sources for the period 1990–2023 were analyzed. As a result, the article presents current data on the prevalence of PCOS using the Rotterdam 2003 definitions, the features of the PCOS incidence in hospital and non-selective (medically unbiased) populations, as well as in various ethnic groups. The review also discusses current guidelines for conducting studies on the PCOS prevalence. Conclusion . The latest guidelines on the diagnosis and management of patients with PCOS, published in 2018, propose to consider the provisions adopted in Rotterdam as the basis for the diagnosis of the syndrome; at the same time, the need to take into account racial and age characteristics is noted.
多囊卵巢综合征(PCOS)被认为是育龄妇女中一种常见的内分泌紊乱，其相关的健康风险贯穿一生。与此同时，该综合征的发病率有很大的差异(4 - 21%)，这反过来又可以通过研究人群特征的影响来解释，包括种族和种族，以及应用的诊断标准。研究的目的。利用2003年鹿特丹标准对育龄妇女多囊卵巢综合征患病率的现有数据进行系统化。材料和方法。利用互联网资源(PubMed, EMBASE, Google Scholar, library)进行信息搜索。分析了1990-2023年期间的文献来源。因此，本文介绍了使用鹿特丹2003年定义的多囊卵巢综合征患病率的当前数据，多囊卵巢综合征在医院和非选择性(医学无偏见)人群以及不同种族群体中的发病率特征。本综述还讨论了目前开展多囊卵巢综合征患病率研究的指导方针。结论。2018年发布的最新PCOS患者诊断和管理指南建议考虑将鹿特丹会议通过的规定作为该综合征诊断的基础;同时，注意到必须考虑到种族和年龄特征。
{"title":"Prevalence of polycystic ovary syndrome in a population of women of reproductive age using Rotterdam 2003 criteria (literature review)","authors":"L. M. Lazareva","doi":"10.29413/abs.2023-8.4.7","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.7","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is considered a common endocrine disorder among women of reproductive age, and the associated health risks persist throughout life. At the same time, there is a wide range of variations in the incidence of the syndrome (4–21 %), which in turn is explained by the influence of the study population characteristics, including ethnicity and race, as well as the applied diagnostic criteria. The aim of the study . To systematize the available data on the prevalence of polycystic ovary syndrome using the Rotterdam 2003 criteria in a population of women of reproductive age. Materials and methods . The search for information was carried out using Internet resources (PubMed, EMBASE, Google Scholar, eLibrary). Literature sources for the period 1990–2023 were analyzed. As a result, the article presents current data on the prevalence of PCOS using the Rotterdam 2003 definitions, the features of the PCOS incidence in hospital and non-selective (medically unbiased) populations, as well as in various ethnic groups. The review also discusses current guidelines for conducting studies on the PCOS prevalence. Conclusion . The latest guidelines on the diagnosis and management of patients with PCOS, published in 2018, propose to consider the provisions adopted in Rotterdam as the basis for the diagnosis of the syndrome; at the same time, the need to take into account racial and age characteristics is noted.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"203 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.29413/abs.2023-8.4.10
T. A. Bairova, O. A. Ershova, A. Yu. Sambyalova, E. V. Belyaeva, V. V. Sinkov, L. V. Rychkova
Background . Obesity is a significant social problem among the population of the world. The leptin gene (LEP) is currently considered as a potential candidate gene influencing metabolic disorders associated with predisposition to overweight and obesity. Leptin plays an important role in body weight homeostasis by influencing food intake and energy expenditure and maintaining constant energy stores. A defect in the leptin gene may be one of the causes of obesity and, as a result, of various obesity-associated pathologies. The aim of the study . To search for single-nucleotide polymorphisms (SNP) of the leptin gene in adolescents with different weight status. Methods . The study involved 20 adolescents aged 11–17 years with normal body weight and overweight/obesity. Research methods: assessment of clinical status with anthropometry; Sanger sequencing of the leptin gene fragment localized in the intron of this gene – (5’-AGCCTTGTTTTCATCATCTGGA, 3’-TGGGAGGAATCGCTCTCAGA). We also carried out bioinformatic processing of sequencing results. Results . As a result of the study, the optimal conditions for amplification of the 891 bps leptin gene region were selected for the above mentioned primer pair of the LEP gene (s16_L891, s16_R891). Based on the results of sequencing, 45 single nucleotide substitutions of the LEP gene were identified, including 23 single nucleotide substitutions which were not previously registered in GenBank. In the group of adolescents with overweight and obesity, 14 unregistered single nucleotide substitutions of the LEP gene and 13 registered SNPs were identified in the GenBank database. In the group of adolescents with normal body weight, these SNPs were not found.
{"title":"Sequencing of a fragment of the leptin gene in adolescents with different weight status","authors":"T. A. Bairova, O. A. Ershova, A. Yu. Sambyalova, E. V. Belyaeva, V. V. Sinkov, L. V. Rychkova","doi":"10.29413/abs.2023-8.4.10","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.10","url":null,"abstract":"Background . Obesity is a significant social problem among the population of the world. The leptin gene (LEP) is currently considered as a potential candidate gene influencing metabolic disorders associated with predisposition to overweight and obesity. Leptin plays an important role in body weight homeostasis by influencing food intake and energy expenditure and maintaining constant energy stores. A defect in the leptin gene may be one of the causes of obesity and, as a result, of various obesity-associated pathologies. The aim of the study . To search for single-nucleotide polymorphisms (SNP) of the leptin gene in adolescents with different weight status. Methods . The study involved 20 adolescents aged 11–17 years with normal body weight and overweight/obesity. Research methods: assessment of clinical status with anthropometry; Sanger sequencing of the leptin gene fragment localized in the intron of this gene – (5’-AGCCTTGTTTTCATCATCTGGA, 3’-TGGGAGGAATCGCTCTCAGA). We also carried out bioinformatic processing of sequencing results. Results . As a result of the study, the optimal conditions for amplification of the 891 bps leptin gene region were selected for the above mentioned primer pair of the LEP gene (s16_L891, s16_R891). Based on the results of sequencing, 45 single nucleotide substitutions of the LEP gene were identified, including 23 single nucleotide substitutions which were not previously registered in GenBank. In the group of adolescents with overweight and obesity, 14 unregistered single nucleotide substitutions of the LEP gene and 13 registered SNPs were identified in the GenBank database. In the group of adolescents with normal body weight, these SNPs were not found.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.29413/abs.2023-8.4.11
E. A. Borodulina, Zh. P. Vasneva, E. S. Vdoushkina, B. E. Borodulin, L. V. Povalyaeva
Background . The stability of human organism for different kind of infection, including SARS-CoV-2 is significantly defined by the immune system. The mechanisms of the cellular immunity to the SARS-CoV-2 are not exactly defined and are under study. The aim . To study the features of cell immunity parameters in patients with lung damage up to 30 % in COVID-19. Material and methods . 73 people were examined during the 2020–2021 pandemic. The study group consisted of 31 patients with lung damage up to 30 % with COVID-19, the comparison group consisted of 42 people not infected with SARS-CoV-2. A complete clinical blood count was carried out using a Medonic M20 hematological analyzer (Boule Medical, Sweden), the level of lymphocyte subpopulations was determined using a FACS Calibur cytometer (BD, USA) and FITC- and phycoerythrin-labeled monoclonal antibodies (Sorbent, Russia). Differences were considered statistically significant at p < 0.05. Results . Patients with COVID-19 with lung damage according to computed tomography (CT) ≤ 30 % before the treatment had a restructuring in the ratio of lymphocyte subpopulations in 67.7 % of cases. Lymphopenia (< 1.1 × 10 9 cells/l) was detected in 34.4 % of patients: a decrease in the absolute count of CD3 + lymphocytes by 30.8 %, CD3 + CD4 + – by 35 %, CD3 + CD8 + – by 6.7 % (p < 0.05), CD16 + CD56 + natural killer (NK) cells – by 29.4 % (p = 0.009). The level of CD95 + lymphocytes in COVID-19 is 3.2 times higher than in healthy individuals. Elevated levels of HLA-DR + - (> 20 %) and CD3 + HLADR + lymphocytes (> 6 %) are recorded in 60 % and 86.7 % of patients, respectively. Elevated levels of CD19 + B lymphocytes (> 17 %) in COVID-19 are 2.6 times more common than in healthy individuals. Correlation dependences of the count of NK cells with a wide range of T lymphocyte subpopulations were revealed. Conclusion . Cellular immunity indicators in COVID-19 have a number of features that can serve as predictors of the progression of the severity of the disease.
{"title":"Features of peripheral blood cellular immunity parameters in patients with lung damage up to 30 % in COVID-19","authors":"E. A. Borodulina, Zh. P. Vasneva, E. S. Vdoushkina, B. E. Borodulin, L. V. Povalyaeva","doi":"10.29413/abs.2023-8.4.11","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.11","url":null,"abstract":"Background . The stability of human organism for different kind of infection, including SARS-CoV-2 is significantly defined by the immune system. The mechanisms of the cellular immunity to the SARS-CoV-2 are not exactly defined and are under study. The aim . To study the features of cell immunity parameters in patients with lung damage up to 30 % in COVID-19. Material and methods . 73 people were examined during the 2020–2021 pandemic. The study group consisted of 31 patients with lung damage up to 30 % with COVID-19, the comparison group consisted of 42 people not infected with SARS-CoV-2. A complete clinical blood count was carried out using a Medonic M20 hematological analyzer (Boule Medical, Sweden), the level of lymphocyte subpopulations was determined using a FACS Calibur cytometer (BD, USA) and FITC- and phycoerythrin-labeled monoclonal antibodies (Sorbent, Russia). Differences were considered statistically significant at p < 0.05. Results . Patients with COVID-19 with lung damage according to computed tomography (CT) ≤ 30 % before the treatment had a restructuring in the ratio of lymphocyte subpopulations in 67.7 % of cases. Lymphopenia (< 1.1 × 10 9 cells/l) was detected in 34.4 % of patients: a decrease in the absolute count of CD3 + lymphocytes by 30.8 %, CD3 + CD4 + – by 35 %, CD3 + CD8 + – by 6.7 % (p < 0.05), CD16 + CD56 + natural killer (NK) cells – by 29.4 % (p = 0.009). The level of CD95 + lymphocytes in COVID-19 is 3.2 times higher than in healthy individuals. Elevated levels of HLA-DR + - (> 20 %) and CD3 + HLADR + lymphocytes (> 6 %) are recorded in 60 % and 86.7 % of patients, respectively. Elevated levels of CD19 + B lymphocytes (> 17 %) in COVID-19 are 2.6 times more common than in healthy individuals. Correlation dependences of the count of NK cells with a wide range of T lymphocyte subpopulations were revealed. Conclusion . Cellular immunity indicators in COVID-19 have a number of features that can serve as predictors of the progression of the severity of the disease.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.29413/abs.2023-8.4.12
Yu. V. Bazhenova, E. Yu. Zorkaltseva, Yu. K. Plotnikova, O. A. Vorobeva
Background . Identification of the characteristics of the pulmonary tuberculosis process using multi-layer spiral computed tomography (MSCT) in patients with tuberculosis and HIV infection is important in the diagnosis of tuberculosis, determining the dissemination of the process and its dynamics during treatment. The aim . To determine the initial characteristics and dynamics of infiltrative tuberculosis according to MSCT in patients with and without HIV infection, with and without multidrug resistance (MDR) of Mycobacterium tuberculosis who were treated in a hospital. Materials and methods . 126 patients aged 19–59 years with tuberculosis, combined with HIV infection and without HIV infection were examined. For statistical processing, we used MS Excel (Microsoft Corp., USA) software package. Results . Patients with tuberculosis and HIV infection in comparison with patients with tuberculosis and without HIV had more expressed intoxication syndrome and respiratory impairement in the clinical picture (p < 0.00001). Patients with coinfection were more likely to suffer from alcohol (p < 0.05) and drug addiction (p < 0.001). According to MSCT, the pathological process in HIV-positive patients with pulmonary tuberculosis was more disseminated (p < 0.05), included severe intrathoracic lymphadenopathy (p < 0.0001), more common pleural lesions (p < 0.005), less common destructive changes (cavities) (p < 0.001) and outcomes in form of fibro-cavernous tuberculosis (p < 0.01). Process regression was slower in patients with tuberculosis and HIV (p < 0.005). According to MSCT, extensive lung damage, intrathoracic lymphadenopathy were more often found in patients with MDR in coinfection (p < 0.05). Cavities and fibro-cavernous tuberculosis outcomes were more common in patients with tuberculosis without HIV infection and with MDR (p < 0.05). Conclusion . MSCT provides detailed information about the pathological process in the lungs and its dynamics under the treatment of tuberculosis and HIV infection.
{"title":"Characteristics of new cases of infiltrative pulmonary tuberculosis in patients having HIV infection with multidrug resistance of the pathogen according to multi-layer spiral computed tomography","authors":"Yu. V. Bazhenova, E. Yu. Zorkaltseva, Yu. K. Plotnikova, O. A. Vorobeva","doi":"10.29413/abs.2023-8.4.12","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.12","url":null,"abstract":"Background . Identification of the characteristics of the pulmonary tuberculosis process using multi-layer spiral computed tomography (MSCT) in patients with tuberculosis and HIV infection is important in the diagnosis of tuberculosis, determining the dissemination of the process and its dynamics during treatment. The aim . To determine the initial characteristics and dynamics of infiltrative tuberculosis according to MSCT in patients with and without HIV infection, with and without multidrug resistance (MDR) of Mycobacterium tuberculosis who were treated in a hospital. Materials and methods . 126 patients aged 19–59 years with tuberculosis, combined with HIV infection and without HIV infection were examined. For statistical processing, we used MS Excel (Microsoft Corp., USA) software package. Results . Patients with tuberculosis and HIV infection in comparison with patients with tuberculosis and without HIV had more expressed intoxication syndrome and respiratory impairement in the clinical picture (p < 0.00001). Patients with coinfection were more likely to suffer from alcohol (p < 0.05) and drug addiction (p < 0.001). According to MSCT, the pathological process in HIV-positive patients with pulmonary tuberculosis was more disseminated (p < 0.05), included severe intrathoracic lymphadenopathy (p < 0.0001), more common pleural lesions (p < 0.005), less common destructive changes (cavities) (p < 0.001) and outcomes in form of fibro-cavernous tuberculosis (p < 0.01). Process regression was slower in patients with tuberculosis and HIV (p < 0.005). According to MSCT, extensive lung damage, intrathoracic lymphadenopathy were more often found in patients with MDR in coinfection (p < 0.05). Cavities and fibro-cavernous tuberculosis outcomes were more common in patients with tuberculosis without HIV infection and with MDR (p < 0.05). Conclusion . MSCT provides detailed information about the pathological process in the lungs and its dynamics under the treatment of tuberculosis and HIV infection.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.29413/abs.2023-8.4.13
V. A. Vasilev, T. T. Karapetyan, V. A. Larionova, I. N. Solyanikova, K. B. Tsekhanovich
Background . People who previously had COVID-19 infection have an increased risk of developing cardiovascular diseases. Left ventricular diastolic dysfunction is an early marker of the development of cardiac pathology. Its early detection is important for the adequate therapy order and dynamic monitoring of patients. In this regard, it seems relevant to study the effect of a recent COVID-19 infection on the left ventricular diastolic function and transmitral blood flow parameters in apparently healthy individuals without clinical and instrumental signs of cardiovascular pathology. The aim of the study . To assess the changes in the diastolic and systolic function of the left ventricle, its anatomical parameters and transmitral blood flow parameters in two groups of apparently healthy individuals: those who had and those who had not COVID-19 infection. Materials and methods . Transthoracic echocardiography was performed according to the standard technique and its results were analyzed in 66 examined patients who were recognized as apparently healthy according to the results of regular comprehensive clinical and instrumental studies. The first group included 30 individuals who underwent an echocardiographic study before or during the COVID-19 pandemic, but did not have a coronavirus infection; the second group consisted of 36 people who recovered from COVID-19. The indicators of the presence of left ventricular diastolic dysfunction and the transmitral blood flow parameters were assessed. The study was approved at a meeting of the Medical Ethics Committee under the Ministry of Health of the Republic of Karelia and of Petrozavodsk State University (Minutes No. 47 of 01.11.2023). Results . The frequency of the left ventricular diastolic dysfunction did not differ in the first and second groups of patients. Statistically significant differences were recorded in the average flow deceleration time for both early and late filling of the left ventricle in people who had and did not have COVID-19 infection. A change in the phase structure of the transmitral blood flow may be an early manifestation of intracardiac hemodynamic disorders in people who have recovered from COVID-19.
{"title":"Left ventricular diastolic dysfunction and transmitral blood flow parameters in patients after COVID-19","authors":"V. A. Vasilev, T. T. Karapetyan, V. A. Larionova, I. N. Solyanikova, K. B. Tsekhanovich","doi":"10.29413/abs.2023-8.4.13","DOIUrl":"https://doi.org/10.29413/abs.2023-8.4.13","url":null,"abstract":"Background . People who previously had COVID-19 infection have an increased risk of developing cardiovascular diseases. Left ventricular diastolic dysfunction is an early marker of the development of cardiac pathology. Its early detection is important for the adequate therapy order and dynamic monitoring of patients. In this regard, it seems relevant to study the effect of a recent COVID-19 infection on the left ventricular diastolic function and transmitral blood flow parameters in apparently healthy individuals without clinical and instrumental signs of cardiovascular pathology. The aim of the study . To assess the changes in the diastolic and systolic function of the left ventricle, its anatomical parameters and transmitral blood flow parameters in two groups of apparently healthy individuals: those who had and those who had not COVID-19 infection. Materials and methods . Transthoracic echocardiography was performed according to the standard technique and its results were analyzed in 66 examined patients who were recognized as apparently healthy according to the results of regular comprehensive clinical and instrumental studies. The first group included 30 individuals who underwent an echocardiographic study before or during the COVID-19 pandemic, but did not have a coronavirus infection; the second group consisted of 36 people who recovered from COVID-19. The indicators of the presence of left ventricular diastolic dysfunction and the transmitral blood flow parameters were assessed. The study was approved at a meeting of the Medical Ethics Committee under the Ministry of Health of the Republic of Karelia and of Petrozavodsk State University (Minutes No. 47 of 01.11.2023). Results . The frequency of the left ventricular diastolic dysfunction did not differ in the first and second groups of patients. Statistically significant differences were recorded in the average flow deceleration time for both early and late filling of the left ventricle in people who had and did not have COVID-19 infection. A change in the phase structure of the transmitral blood flow may be an early manifestation of intracardiac hemodynamic disorders in people who have recovered from COVID-19.","PeriodicalId":32463,"journal":{"name":"Acta Biomedica Scientifica","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135745160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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