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Supercritical Carbon Dioxide Extraction of Bioactive Compounds from Feijoa (Feijoa sellowiana) Leaves 超临界二氧化碳萃取飞荷叶中生物活性物质的研究
Pub Date : 2018-06-01 DOI: 10.29252/NFSR.5.3.23
M. Mousavi, Mandana Bimakr, S. M. Ghoreishi, A. Ganjloo
Background and Objectives: Supercritical fluid extraction (SFE) technique has been studied for extraction of bioactive compounds from Feijoa sellowiana leaves. Results were compared with those obtained from conventional ethanolic extraction (CE). Materials and Methods: Results: The best supercritical carbon dioxide (SC-CO2) extraction conditions was determined as 250 bar of pressure, 50 °C of temperature, and 90 min dynamic extraction time. Under these conditions, the crude extraction yield (CEY) of bioactive compounds was 38.14 ± 0.17 mg g. Spectrophotometric analysis revealed that the extract possesses strong radical scavenging activity (78.18 ± 0.12%, 74.19 ± 0.14%, 49.38 ± 0.18% inhibition of DPPH ̇, ABTS ̇, and OH ̇, respectively). The HPLC analysis revealed that gallic acid, catechin, rutin, ferulic acid, apigenin, and quercetin are the major phenolic compounds present in the extract. The CEY obtained using the best conditions of SCE process was around 70% of those obtained with CE. However, the quality of extracts regarding radical scavenging activity and bioactive phenolic content was higher than those obtained by CE. Conclusions: F. sellowiana leave is a potential source of bioactive compounds with strong radical scavenging activity and the SC-CO2 extraction can be considered as a green technique to extract bioactive compounds.
背景与目的:研究了超临界流体萃取(SFE)技术从黄飞蒿叶中提取生物活性物质。结果与常规乙醇提取法(CE)进行了比较。材料与方法:结果:超临界二氧化碳(SC-CO2)提取的最佳条件为压力250 bar,温度50℃,动态提取时间90 min。在此条件下,活性物质的粗提率(CEY)为38.14±0.17 mg g,对DPPH、ABTS、OH的抑制率分别为78.18±0.12%、74.19±0.14%、49.38±0.18%。高效液相色谱分析表明,未食子酸、儿茶素、芦丁、阿魏酸、芹菜素和槲皮素是提取物中主要的酚类化合物。采用最佳SCE工艺条件得到的CEY约为CE法的70%。然而,在自由基清除活性和生物活性酚含量方面,提取物的质量高于CE法。结论:黄连叶具有较强的自由基清除活性,是黄连叶生物活性物质的潜在来源,SC-CO2提取技术可作为提取黄连叶生物活性物质的绿色技术。
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引用次数: 9
Production of a Semi Ready-to-Eat Shrimp Soup Powder and Assessment of Its Shelf Life 一种半即食虾汤粉的生产及保质期评价
Pub Date : 1900-01-01 DOI: 10.29252/NFSR.6.1.41
Ghorban Zarehgashti, Y. Etemadian, A. Valipour, M. Rahnama, F. Khodabandeh, A. Fahim, Young Researches
Background and Objectives: Macrobrachium nipponense are wetland crustaceans. The shrimp is rich in protein and unsaturated fatty acids (FAs). Therefore, the aim of this study was to use M. nipponense as a supplement for the preparation of a new soup. Materials and Methods: Nearly 40 kg of M. nipponense were harvested from Anzali Lagoon, Northern Iran. Shrimps were washed and dipped in NaCl solution (10% w/v) with a ratio of 1 to 2 at boiling temperature for 3 min. Then, these were cooled down for 10 minutes and dried by a cabinet-type air dryer at 70 C for 6 h. Dried meats of shrimps were crushed using grinder. Nearly 4% of the crushes were combined with other ingreadients of the shrimp soup. Each 100 g of the soup powder was packed in a metalized film under vacuum and stored at room temperature for 6 months of storage. Results: Results have shown that dried shrimp meats include useful compositions such as saturated FAs (ΣSFA; 33.36 ±2.5%), monounsaturated FAs (ΣMUFA; 21±1.6%) and polyunsaturated FAs (ΣPUFA; 38.92 ±2.1%). The protein content in dried shrimp meats was high (72.74 ±1.99% of dry weight). Chemical parameters of the samples such as thiobarbituric acid (TBA), peroxide value (PV), total volatile base nitrogen (TVB-N) and free fatty acid (FFA) increased and quality of the packaged soup color decreased with increased storage time (P < 0.05). The sensory evaluation (color, odor, flavor/taste and texture) of the samples has shown that these parameters are more acceptable during the first month of storage than other months. The total number of bacteria and fungi was respectively calculated as 4.1 and 3.79 cfu/g of sample during 6 months of storage. Conclusions: In this study, use of 4% of the dried shrimp meats as supplement made a great taste in the soup. Furthermore, appropriate packaging under vacuum and use of metalized polyethylene films increased the shelf-life of the soup powder.
背景与目的:日本沼虾是湿地甲壳类动物。虾富含蛋白质和不饱和脂肪酸(FAs)。因此,本研究的目的是利用日本木参作为一种新的汤的补充制备。材料和方法:从伊朗北部的Anzali泻湖收获近40公斤日本血吸虫。将虾仁洗净,以1比2的NaCl溶液(10% w/v)浸泡在沸水中3 min,冷却10 min后,用柜式空气干燥机在70℃下干燥6 h,虾仁干肉用粉碎机粉碎。近4%的碎虾与虾汤的其他配料混合在一起。将每100g的汤粉真空包装在金属化薄膜中,在室温下保存6个月。结果:结果表明,虾干肉含有有益的成分,如饱和脂肪酸(ΣSFA;33.36±2.5%),单不饱和脂肪酸(ΣMUFA;21±1.6%)和多不饱和脂肪酸(ΣPUFA;38.92±2.1%)。虾干肉的蛋白质含量较高(占干重的72.74±1.99%)。随着贮藏时间的延长,样品的硫代巴比妥酸(TBA)、过氧化值(PV)、总挥发性碱氮(TVB-N)和游离脂肪酸(FFA)等化学参数升高,包装汤色质量下降(P < 0.05)。样品的感官评价(颜色、气味、风味/味道和质地)表明,这些参数在储存的第一个月比其他月份更容易接受。在6个月的保存期间,细菌和真菌的总数分别为4.1和3.79 cfu/g。结论:在本研究中,使用4%的虾干肉作为补充,使汤的味道很好。此外,适当的真空包装和金属化聚乙烯薄膜的使用增加了汤粉的保质期。
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引用次数: 5
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Nutrition and Food Sciences Research
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