Pub Date : 2023-04-29DOI: 10.51804/jsh.v7i1.6731.9-14
Indra Adi Wira Prasetya, Aziz Aziz, Evy Ratnasari Ekawati, Muhammad Sungging Pradana, Nadiah Al Batati, Esti Rizkiana Pratiwi
Lime (Citrus aurantifolia) is one of the herbal plants that is rich in benefits, especially as a herbal medicines. Besides of lime fruit, lime peel also has antibacterial properties such as essential oils which contain flavonoids which can inhibit bacterial growth. This research utilizes lime peel which will be extracted as a test material. The purpose of this research is to determine the antibacterial potential of lime peel extract against Vibrio sp. at various concentrations. This research used the well-diffusion method using MHA as a test medium to determine whether lime peel extract can inhibit the growth of Vibrio sp. Various concentrations and penicillin as a positive control used in this research to determine which concentration has a greatest potential for inhibiting the growth of Vibrio sp. Result of this study used qualitative analysis by comparing the clear zone size produced on the medium. The obtained result shown that 20%, 40%, 60%, 80% and 100% concentration of lime peel can forming a clear zone which mean they can inhibit the growth of Vibrio sp. The best concentration used to inhibit Vibrio sp. is 100% concentration which produce 23.7 mm clear zone on MHA medium. Lime peel extract can be used to inhibit Vibrio sp. growth based on the research result.
{"title":"Uji Potensi Antibakteri Ekstrak Kulit Jeruk Nipis Citrus aurantifolia (Christm) Swingle Terhadap Vibrio sp.","authors":"Indra Adi Wira Prasetya, Aziz Aziz, Evy Ratnasari Ekawati, Muhammad Sungging Pradana, Nadiah Al Batati, Esti Rizkiana Pratiwi","doi":"10.51804/jsh.v7i1.6731.9-14","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.6731.9-14","url":null,"abstract":"Lime (Citrus aurantifolia) is one of the herbal plants that is rich in benefits, especially as a herbal medicines. Besides of lime fruit, lime peel also has antibacterial properties such as essential oils which contain flavonoids which can inhibit bacterial growth. This research utilizes lime peel which will be extracted as a test material. The purpose of this research is to determine the antibacterial potential of lime peel extract against Vibrio sp. at various concentrations. This research used the well-diffusion method using MHA as a test medium to determine whether lime peel extract can inhibit the growth of Vibrio sp. Various concentrations and penicillin as a positive control used in this research to determine which concentration has a greatest potential for inhibiting the growth of Vibrio sp. Result of this study used qualitative analysis by comparing the clear zone size produced on the medium. The obtained result shown that 20%, 40%, 60%, 80% and 100% concentration of lime peel can forming a clear zone which mean they can inhibit the growth of Vibrio sp. The best concentration used to inhibit Vibrio sp. is 100% concentration which produce 23.7 mm clear zone on MHA medium. Lime peel extract can be used to inhibit Vibrio sp. growth based on the research result.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135847630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.51804/jsh.v7i1.6847.35-40
Gland Konay Octavianus, Abdul Chamid, Dheasy Herawati
Dengue hemorrhagic fever (DHF) is a disease transmitted by the bite of a female Aedes aegypti mosquito infected with the dengue virus. The essential oil contained in lemon grass has antioxidant activity and contains active compounds that are toxic as larvicides. The purpose of this study was to determine the effectiveness, and the effective concentration of the Ethanol and N-Hexan extracts of lemon grass (Cymbopogon ciratus) leaves. The research subjects were 300 instar III Aedes aegypti larvae containing 25 larvae at each concentration with each concentration group namely 30 ppm, 35 ppm, 40 ppm, 45 ppm, and 50 ppm, which were observed for 1 hour, 3 hours, 6 hours, and 24 hours. From the results of the ethanol extract of citronella leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm and can kill 14 Aedes aegypti larvae. Whereas in the N-Hexan extract of lemongrass leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm which can kill 11 Aedes aegypti larvae. Lemongrass leaves (Cymbopogon ciratus) are effectively used as natural larvicides. Effective concentrations are 35 ppm, 40 ppm, 45 ppm, 50 ppm for 24 hours. Keywords: Aedes aegypti, Dengue Hemorrhagic Fever, Lemongrass (Cymbopogon ciratus) leaves.
{"title":"Pengaruh Ekstrak Ethanol dan N-Hexan Daun Serai (Cymbopogon citratus) Terhadap Larva Nyamuk Aedes aegypti","authors":"Gland Konay Octavianus, Abdul Chamid, Dheasy Herawati","doi":"10.51804/jsh.v7i1.6847.35-40","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.6847.35-40","url":null,"abstract":"Dengue hemorrhagic fever (DHF) is a disease transmitted by the bite of a female Aedes aegypti mosquito infected with the dengue virus. The essential oil contained in lemon grass has antioxidant activity and contains active compounds that are toxic as larvicides. The purpose of this study was to determine the effectiveness, and the effective concentration of the Ethanol and N-Hexan extracts of lemon grass (Cymbopogon ciratus) leaves. The research subjects were 300 instar III Aedes aegypti larvae containing 25 larvae at each concentration with each concentration group namely 30 ppm, 35 ppm, 40 ppm, 45 ppm, and 50 ppm, which were observed for 1 hour, 3 hours, 6 hours, and 24 hours. From the results of the ethanol extract of citronella leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm and can kill 14 Aedes aegypti larvae. Whereas in the N-Hexan extract of lemongrass leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm which can kill 11 Aedes aegypti larvae. Lemongrass leaves (Cymbopogon ciratus) are effectively used as natural larvicides. Effective concentrations are 35 ppm, 40 ppm, 45 ppm, 50 ppm for 24 hours. Keywords: Aedes aegypti, Dengue Hemorrhagic Fever, Lemongrass (Cymbopogon ciratus) leaves.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135847627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-29DOI: 10.51804/jsh.v7i1.14321.47-50
Siti Nur Husnul Yusmiati, Aprilia Aly Damayanti, Christina Destri Wiwis Wijayanti, Tonny Watoyani
The body's immune system is a system that detects a wide variety of outside biological influences, organisms will protect the body from infections, bacteria, viruses to parasites. During the current pandemic, the immune system is very important to prevent exposure to COVID-19. Efforts are made to be able to sufficient intake of vitamins and vaccinations. Vitamin D is one that is needed to prevent exposure to viruses. This study aims to determine vitamin D levels in sinovac vaccine recipients as an illustration of the immune system. This scientific paper research uses primary data analytic observation method with a cross-sectional design supported by literature studies, namely by describing the results of the research in the form of a percentage (%) decrease and increase in vitamin D compared to normal values. The results of the research on vitamin D levels receiving the Sinovac vaccine found that 92% or 23 patients out of 25 patients showed vitamin D levels were still within normal values and 8% or 2 patients showed vitamin D levels below normal values. It can be concluded that some of the research samples were in the normal range so that the immune system as the response of vitamin D to vaccines is still being seen. Keywords : SINOVAC Vaccine, Vitamin D, Immune System.
{"title":"ANALISIS KADAR VITAMIN D PADA PENERIMA VAKSIN SINOVAC SEBAGAI GAMBARAN SISTEM IMUNITAS","authors":"Siti Nur Husnul Yusmiati, Aprilia Aly Damayanti, Christina Destri Wiwis Wijayanti, Tonny Watoyani","doi":"10.51804/jsh.v7i1.14321.47-50","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.14321.47-50","url":null,"abstract":"The body's immune system is a system that detects a wide variety of outside biological influences, organisms will protect the body from infections, bacteria, viruses to parasites. During the current pandemic, the immune system is very important to prevent exposure to COVID-19. Efforts are made to be able to sufficient intake of vitamins and vaccinations. Vitamin D is one that is needed to prevent exposure to viruses. This study aims to determine vitamin D levels in sinovac vaccine recipients as an illustration of the immune system. This scientific paper research uses primary data analytic observation method with a cross-sectional design supported by literature studies, namely by describing the results of the research in the form of a percentage (%) decrease and increase in vitamin D compared to normal values. The results of the research on vitamin D levels receiving the Sinovac vaccine found that 92% or 23 patients out of 25 patients showed vitamin D levels were still within normal values and 8% or 2 patients showed vitamin D levels below normal values. It can be concluded that some of the research samples were in the normal range so that the immune system as the response of vitamin D to vaccines is still being seen. Keywords : SINOVAC Vaccine, Vitamin D, Immune System.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134952237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-29DOI: 10.51804/jsh.v7i1.6845.22-27
Muhammad Sungging Pradana, Titania Wahyu Eka Dinata, Abdul Chamid
Head lice (Pediculus humanus capitis) commonly infecting children in most countries. Soursop leaves (Annona muricata Linn) contain acetogenin compounds. The acetogenin compound functions as an anti feedent, some people also use olive oil to kill head lice, because olive oil contains flavonoids. The purpose of this study was to see the potential of soursop leaf extract with olive oil to kill head lice. This type of research is experimental. The method used to obtain soursop leaf extract is the maceration method. The results of giving soursop leaf extract (Annona muricata Linn) with olive oil (Olea eruopaea) on the mortality of head lice (Pediculus humanus capitis) in controls showed no dead head lice, 10% concentration of 4 dead head lice, 20% concentration 6 dead head lice, 30% concentration of 8 dead head lice observed every 15 minutes. Based on these results, it can be seen that the combination of soursop leaf extract and olive oil has the potential to kill head lice.Keywords: Soursop leaf extract, olive oil, head lice
{"title":"Uji Potensi Ekstrak Daun Sirsak (Annona muricata linn) Dengan Minyak Zaitun Terhadap Daya Bunuh Kutu Rambut (Pediculus humanus capitis)","authors":"Muhammad Sungging Pradana, Titania Wahyu Eka Dinata, Abdul Chamid","doi":"10.51804/jsh.v7i1.6845.22-27","DOIUrl":"https://doi.org/10.51804/jsh.v7i1.6845.22-27","url":null,"abstract":"Head lice (Pediculus humanus capitis) commonly infecting children in most countries. Soursop leaves (Annona muricata Linn) contain acetogenin compounds. The acetogenin compound functions as an anti feedent, some people also use olive oil to kill head lice, because olive oil contains flavonoids. The purpose of this study was to see the potential of soursop leaf extract with olive oil to kill head lice. This type of research is experimental. The method used to obtain soursop leaf extract is the maceration method. The results of giving soursop leaf extract (Annona muricata Linn) with olive oil (Olea eruopaea) on the mortality of head lice (Pediculus humanus capitis) in controls showed no dead head lice, 10% concentration of 4 dead head lice, 20% concentration 6 dead head lice, 30% concentration of 8 dead head lice observed every 15 minutes. Based on these results, it can be seen that the combination of soursop leaf extract and olive oil has the potential to kill head lice.Keywords: Soursop leaf extract, olive oil, head lice","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134952236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-07DOI: 10.51804/jsh.v3i2.598.9-16
Handono Fatkhur Rahman, N. Sholihah, S. Nugroho
Increased intradialysis weight gain increases the risk of cardiovascular death. Limiting fluid intake was very important to do because the kidneys cannot function normally. An increased intradialysis weight gain over 5% of dry weight can cause any complication. An internal factor that affects a person in performing self-care on fluid intake restriction, in this case is self-efficacy. The purpose of this study to determine the relationship of self-efficacy and self-care fluid intake with increased intradialysis weight gain. This research is a quantitative research, with cross sectional approach. Number of samples selected with random sampling was 112 respondents, primary data collection with questionnaires and weight scale. Statistical analysis used pearson product moment and multiple linier regression. The result of pearson product moment analysis shows p-value of self-efficay was 0,000 and p-value of self-care fluid intake was 0,038 means that each variables has a statistically significant relationship with increased intradialisis weight gain. The result of multiple linier regression analysis shows coefficient of determination value 0,138, means 13,8% self-efficacy and self-care fluid intake variables determine increased intradialysis weight gain, while 86,2% is determined by other variables not included in this research. Conclusion from this research is there is relationship between self-efficacy and self-care fluid intake to increased intradialysis weight gain with negative correlation coefficient, Means the stronger self-efficay and the better behavior of self-care fluid intake of hemodialysis clients will decrease the increase in intradialisis weight gain.
{"title":"Efikasi Diri, Perawatan Diri Asupan Cairan, Peningkatan Berat Badan Intradialisis Klien Hemodialisis","authors":"Handono Fatkhur Rahman, N. Sholihah, S. Nugroho","doi":"10.51804/jsh.v3i2.598.9-16","DOIUrl":"https://doi.org/10.51804/jsh.v3i2.598.9-16","url":null,"abstract":"Increased intradialysis weight gain increases the risk of cardiovascular death. Limiting fluid intake was very important to do because the kidneys cannot function normally. An increased intradialysis weight gain over 5% of dry weight can cause any complication. An internal factor that affects a person in performing self-care on fluid intake restriction, in this case is self-efficacy. The purpose of this study to determine the relationship of self-efficacy and self-care fluid intake with increased intradialysis weight gain. This research is a quantitative research, with cross sectional approach. Number of samples selected with random sampling was 112 respondents, primary data collection with questionnaires and weight scale. Statistical analysis used pearson product moment and multiple linier regression. The result of pearson product moment analysis shows p-value of self-efficay was 0,000 and p-value of self-care fluid intake was 0,038 means that each variables has a statistically significant relationship with increased intradialisis weight gain. The result of multiple linier regression analysis shows coefficient of determination value 0,138, means 13,8% self-efficacy and self-care fluid intake variables determine increased intradialysis weight gain, while 86,2% is determined by other variables not included in this research. Conclusion from this research is there is relationship between self-efficacy and self-care fluid intake to increased intradialysis weight gain with negative correlation coefficient, Means the stronger self-efficay and the better behavior of self-care fluid intake of hemodialysis clients will decrease the increase in intradialisis weight gain.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126708985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-07DOI: 10.51804/jsh.v3i2.599.17-21
Fines Dwi Martanda
Margarine is a product of emulsion, both semisolid and liquid made from vegetable fats. The composition of margarine includes fats, water, vitamins A and vitamin E as well as other ingredients. At SNI 7388:2009 on the maximum limit of microbial contamination there are some bacteria that are often found in foodstuffs margarine including Salmonella sp. and Staphylococcus aureus. Salmonellasp. bacteria can lead to deman enteric, diarrhea, nausea, stomach cramps. Staphylococcus aureusbacteria are the most frequently used agents to cause skin sepsis, enteric infections, septic and pneumonia. The purpose of research is to know the contamination of Salmonella sp. and Staphylococcus aureus as well as ALT bacteria in margarine. The method used is laboratory experiments. From the results of the identification of 10 samples of Salmonella sp. and Staphylococcus aureus bacteria. As well as the result ALT bacteria meet the quality requirements of margarine.
{"title":"IDENTIFIKASI Salmonella sp. Dan Staphylococcus aureus Serta HITUNG JUMLAH TOTAL BAKTERI PADA MARGARIN","authors":"Fines Dwi Martanda","doi":"10.51804/jsh.v3i2.599.17-21","DOIUrl":"https://doi.org/10.51804/jsh.v3i2.599.17-21","url":null,"abstract":"Margarine is a product of emulsion, both semisolid and liquid made from vegetable fats. The composition of margarine includes fats, water, vitamins A and vitamin E as well as other ingredients. At SNI 7388:2009 on the maximum limit of microbial contamination there are some bacteria that are often found in foodstuffs margarine including Salmonella sp. and Staphylococcus aureus. Salmonellasp. bacteria can lead to deman enteric, diarrhea, nausea, stomach cramps. Staphylococcus aureusbacteria are the most frequently used agents to cause skin sepsis, enteric infections, septic and pneumonia. The purpose of research is to know the contamination of Salmonella sp. and Staphylococcus aureus as well as ALT bacteria in margarine. The method used is laboratory experiments. From the results of the identification of 10 samples of Salmonella sp. and Staphylococcus aureus bacteria. As well as the result ALT bacteria meet the quality requirements of margarine.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115192317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-07DOI: 10.51804/jsh.v3i2.601.37-41
Nurfadilah Nurfadilah, Anton Yuntarso, Dheasy Herawati
Carbohydrates are a source of energy needed by the body because carbohydrates are an important food component. According to SNI (Standar Nasional Indonesia), carbohydrate content in food must not be less than 60%. In this study, researchers wanted to compare the method of determining carbohydrate content according to SNI and according to AOAC (Non-SNI), both methods used Luff Schoorl, in the SNI method there was an addition of CH3COOH, whereas the Non-SNI method did not add CH3COOH. In this study a sample of a mixture of glucose, sucrose and NaCl was used. From the research results obtained indicate that there is a difference between the SNI method and Non-SNI method, where the Non-SNI method is better used at a 10% sample concentration with 250X dilution and 20% sample concentration at 500x dilution, whereas on the SNI method is better used at 30% sample concentration with 500X dilution, and the accuracy value obtained is 94.14% to 97.23%.
碳水化合物是人体所需能量的来源，因为碳水化合物是重要的食物成分。根据SNI (standard Nasional Indonesia)，食品中的碳水化合物含量不得低于60%。在本研究中，研究者想比较根据SNI和根据AOAC (Non-SNI)测定碳水化合物含量的方法，两种方法都使用了Luff Schoorl, SNI方法中添加了CH3COOH，而Non-SNI方法没有添加CH3COOH。在本研究中，使用了葡萄糖、蔗糖和NaCl的混合物样品。从得到的研究结果来看，SNI方法与非SNI方法存在差异，其中非SNI方法在10%的样品浓度、250倍的稀释和20%的样品浓度、500倍的稀释下使用效果较好，而SNI方法在30%的样品浓度、500倍的稀释下使用效果较好，得到的准确度值为94.14% ~ 97.23%。
{"title":"PERBANDINGAN METODE STANDAR NASIONAL INDONESIA DAN NON STANDAR NASIONAL INDONESIA DALAM PENENTUAN KADAR KARBOHIDRAT TOTAL","authors":"Nurfadilah Nurfadilah, Anton Yuntarso, Dheasy Herawati","doi":"10.51804/jsh.v3i2.601.37-41","DOIUrl":"https://doi.org/10.51804/jsh.v3i2.601.37-41","url":null,"abstract":"Carbohydrates are a source of energy needed by the body because carbohydrates are an important food component. According to SNI (Standar Nasional Indonesia), carbohydrate content in food must not be less than 60%. In this study, researchers wanted to compare the method of determining carbohydrate content according to SNI and according to AOAC (Non-SNI), both methods used Luff Schoorl, in the SNI method there was an addition of CH3COOH, whereas the Non-SNI method did not add CH3COOH. In this study a sample of a mixture of glucose, sucrose and NaCl was used. From the research results obtained indicate that there is a difference between the SNI method and Non-SNI method, where the Non-SNI method is better used at a 10% sample concentration with 250X dilution and 20% sample concentration at 500x dilution, whereas on the SNI method is better used at 30% sample concentration with 500X dilution, and the accuracy value obtained is 94.14% to 97.23%.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130831127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-07DOI: 10.51804/jsh.v3i2.600.22-32
Ary Andini
Tetracycline are antibiotics with broad-spectrum that used routinely in veterinary medicine for animal treatment and prevention from infectious diseases. But, consuming antibiotics for long time and exceeding standards can lead to accumulation of antibiotic residues in the body. Based on SNI 01-6366-2000, the maximum residual limit (BMR) of tetracycline antibiotic is 0.1 mg/kg in meat.The purpose of this study was to determine the concentration of tetracycline residues in broiler chicken meat and beef that obtained in East Surabaya namely Manyar market, Sinar Baru market, Ampengan Batu market, Tenggilis Mejoyo market, Krempyeng market, Soponyono market, and Gunung Anyar market. This study used Thin Layer Chromatography (TLC) as qualitative test and Spectrophotometric method as a quantitative test to determine the residual levels of tetracycline antibiotics. This study was conducted on March 15 onto May 2 2019. Based on the study, about 32 broiler chicken and 13 beef samples detected by the TLC method showed that had negative results, there was no containing tetracycline residues in samples. Awhile the results of the spectrophotometric method showed that as many as 31 samples of broiler chicken and 13 beef samples showed that had positive results containing residues of tetracycline antibiotics that exceed of the standard. Based on the results obtained that average concentration of tetracycline in samples of broiler chicken meat about 1,7 mg/kg and 0,8 mg/kg in beef samples. Descriptively,tetracycline residues in broiler chicken meat were higher than beef.
{"title":"ANALISIS RESIDU ANTIBIOTIK TETRASIKLIN PADA DAGING AYAM BROIlER DAN DAGING SAPI","authors":"Ary Andini","doi":"10.51804/jsh.v3i2.600.22-32","DOIUrl":"https://doi.org/10.51804/jsh.v3i2.600.22-32","url":null,"abstract":"Tetracycline are antibiotics with broad-spectrum that used routinely in veterinary medicine for animal treatment and prevention from infectious diseases. But, consuming antibiotics for long time and exceeding standards can lead to accumulation of antibiotic residues in the body. Based on SNI 01-6366-2000, the maximum residual limit (BMR) of tetracycline antibiotic is 0.1 mg/kg in meat.The purpose of this study was to determine the concentration of tetracycline residues in broiler chicken meat and beef that obtained in East Surabaya namely Manyar market, Sinar Baru market, Ampengan Batu market, Tenggilis Mejoyo market, Krempyeng market, Soponyono market, and Gunung Anyar market. This study used Thin Layer Chromatography (TLC) as qualitative test and Spectrophotometric method as a quantitative test to determine the residual levels of tetracycline antibiotics. This study was conducted on March 15 onto May 2 2019. Based on the study, about 32 broiler chicken and 13 beef samples detected by the TLC method showed that had negative results, there was no containing tetracycline residues in samples. Awhile the results of the spectrophotometric method showed that as many as 31 samples of broiler chicken and 13 beef samples showed that had positive results containing residues of tetracycline antibiotics that exceed of the standard. Based on the results obtained that average concentration of tetracycline in samples of broiler chicken meat about 1,7 mg/kg and 0,8 mg/kg in beef samples. Descriptively,tetracycline residues in broiler chicken meat were higher than beef.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123734795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-06DOI: 10.51804/JSH.V3I2.597.1-8
Endah Prayekti, T. Sumarsono
Tofu waste is generally used for animal feed, potential for biogas production, and as a traditional food. Opportunities for the use of tofu waste are very wide because of the high nutrient content of tofu waste. Tofu waste nutrient content has the potential to be utilized as an alternative medium for mold growth. One type of mold that is often analyse in the laboratory is from the genusPenicillium. This study aims to determine the composition of an alternative growth media from tofu waste for Penicillium spp. This study is an experimental laboratory with the independent variable of tofu waste mass (0,1,2,3,4,5 gr) and sucrose mass (0,2,4,6 gr), while the dependent variable observed from this study includes the amount Penicillium spp colonies and their growth characteristics in tofu waste media. Both dependent variable observed then compared with Potato Dextrose Agar (PDA) as commercial media Tofu waste composition used in this study were (gr/100mL) : tofu waste (0, 1, 2, 3, 4, 5 gr), sucrose (0, 2, 4, 6 gr), aquadest (100mL), and agar powder (1,5gr). Statistical results using the Kruskal-Wallis Test showed the difference of the use of sucrose 2 and 6 gr in the media on thePenicillium spp colonies number. Colony morphology showed better performance at 5 gr of tofu waste mass. Morphological analysis shows the differences in morphological characters which are quite far from commercial media as a control using PDA. As conclusion, tofu waste media can be used to growPenicillium spp, but it is necessary to add some components both in the form of elements and compounds to tofu waste media to be able to display results that can approach commercial media.
{"title":"ANALISIS JUMLAH DAN MORFOLOGI Penicillium spp PADA MEDIA AMPAS TAHU","authors":"Endah Prayekti, T. Sumarsono","doi":"10.51804/JSH.V3I2.597.1-8","DOIUrl":"https://doi.org/10.51804/JSH.V3I2.597.1-8","url":null,"abstract":"Tofu waste is generally used for animal feed, potential for biogas production, and as a traditional food. Opportunities for the use of tofu waste are very wide because of the high nutrient content of tofu waste. Tofu waste nutrient content has the potential to be utilized as an alternative medium for mold growth. One type of mold that is often analyse in the laboratory is from the genusPenicillium. This study aims to determine the composition of an alternative growth media from tofu waste for Penicillium spp. This study is an experimental laboratory with the independent variable of tofu waste mass (0,1,2,3,4,5 gr) and sucrose mass (0,2,4,6 gr), while the dependent variable observed from this study includes the amount Penicillium spp colonies and their growth characteristics in tofu waste media. Both dependent variable observed then compared with Potato Dextrose Agar (PDA) as commercial media Tofu waste composition used in this study were (gr/100mL) : tofu waste (0, 1, 2, 3, 4, 5 gr), sucrose (0, 2, 4, 6 gr), aquadest (100mL), and agar powder (1,5gr). Statistical results using the Kruskal-Wallis Test showed the difference of the use of sucrose 2 and 6 gr in the media on thePenicillium spp colonies number. Colony morphology showed better performance at 5 gr of tofu waste mass. Morphological analysis shows the differences in morphological characters which are quite far from commercial media as a control using PDA. As conclusion, tofu waste media can be used to growPenicillium spp, but it is necessary to add some components both in the form of elements and compounds to tofu waste media to be able to display results that can approach commercial media.","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130975848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-23DOI: 10.51804/jsh.v3i1.313.45-54
Afidatul Muadifah, Helda Wika Amini, A. E. Putri, N. Latifah
Indonesia merupakan negara yang memiliki berbagai macam tanaman herbal berkhasiat obat. Salah satu jenis tanaman obat yang sering digunakan untuk pengobatan adalah rimpang kunyit. Rimpang kunyit mengandung senyawa alkaloid, tanin dan flavonoid yang telah terbukti sebagai antibakteri. Penelitian ini dilakukan secara in vitro untuk mengetahui kandungan senyawa dan aktivitas ekstrak rimpang kunyit yang dilanjutkan dengan pengujian menggunakan metode Kadar Hambat Minimum (KHM) terhadap bakteri Staphylococcus aureus. Penyarian ekstrak rimpang kunyit dilakukan dengan cara sokhletasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi dengan variasi konsentrasi 45%, 55%, 65% dan 75%. Hasil penelitian ini menunjukkan bahwa ekstrak rimpang kunyit 45% merupakan konsentrasi minimum yang memiliki aktivitas antibakteri terhadap S.aureus sebesar 12,5 mm
{"title":"AKTIVITAS ANTIBAKTERI EKSTRAK RIMPANG KUNYIT (Curcuma domestica Val) TERHADAP BAKTERI Staphylococcus aureus","authors":"Afidatul Muadifah, Helda Wika Amini, A. E. Putri, N. Latifah","doi":"10.51804/jsh.v3i1.313.45-54","DOIUrl":"https://doi.org/10.51804/jsh.v3i1.313.45-54","url":null,"abstract":"Indonesia merupakan negara yang memiliki berbagai macam tanaman herbal berkhasiat obat. Salah satu jenis tanaman obat yang sering digunakan untuk pengobatan adalah rimpang kunyit. Rimpang kunyit mengandung senyawa alkaloid, tanin dan flavonoid yang telah terbukti sebagai antibakteri. Penelitian ini dilakukan secara in vitro untuk mengetahui kandungan senyawa dan aktivitas ekstrak rimpang kunyit yang dilanjutkan dengan pengujian menggunakan metode Kadar Hambat Minimum (KHM) terhadap bakteri Staphylococcus aureus. Penyarian ekstrak rimpang kunyit dilakukan dengan cara sokhletasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi dengan variasi konsentrasi 45%, 55%, 65% dan 75%. Hasil penelitian ini menunjukkan bahwa ekstrak rimpang kunyit 45% merupakan konsentrasi minimum yang memiliki aktivitas antibakteri terhadap S.aureus sebesar 12,5 mm","PeriodicalId":326360,"journal":{"name":"Jurnal SainHealth","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114332714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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