Pub Date : 2023-12-01DOI: 10.52418/moldovan-med-j.66-2.23.08
Valeriu David, V. Fulga, V. Petrovici, Ecaterina Carpenco, L. Saptefrati
Background: A normal maternal-fetal system is essential for the functioning of the placenta during placentation and the establishment of the maternalembryofetal vascular circulation. The angiopoietin/TIE pathway is involved in vascular morphogenesis through regulation, survival and maturation of endothelial cells concomitant with vascular remodeling. Deregulation of pro-angiogenic factor secretion and expression is associated with disruption of vascular morphogenesis, reduction of vascular bed and installation of primary placental insufficiency. The aim: Evaluation of Ang 1 immunoexpression in early term compromised pregnancies in the context of chorio-villary circulatory dysfunction in primary placental insufficiency. Material and methods: Abortion product from 61 patients (stagnant pregnancies – 39 cases, early miscarriage – 8 cases, control group – 14 cases of pregnancies solved on social indications/ desire) were immunohistochemically evaluated with the marker for anti-Ang1 and anti-CD31. Results: The villous syncytiotrophoblast was the most immunoreactive area. Most of cases of the pregnancies terminated on social indications/ desire were anti-Ang1 negative. The levels of anti-Ang1 immunoexpression were statistically significantly different in case of syncytiotrophoblast of early miscarriages and abortions terminated on social indications/ desire. The highest chorio-villous vascular density was noticed in the abortions on social indications/ desire and early misscariages group. Conclusions: The placental period is characterized by a weak angiogenic Ang1 differentiated cellular environment in the chorio-villous germinal site in the group of short term compromised pregnancies. The selectively immunoexpressed cellular profile statistically significantly correlates with placental vascular index and chorio-villous vascular density in stagnant pregnancies.
{"title":"Ang1 immunoexpression vs vascular profile in chorio-villous germinative status in early term compromised pregnancies","authors":"Valeriu David, V. Fulga, V. Petrovici, Ecaterina Carpenco, L. Saptefrati","doi":"10.52418/moldovan-med-j.66-2.23.08","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.66-2.23.08","url":null,"abstract":"Background: A normal maternal-fetal system is essential for the functioning of the placenta during placentation and the establishment of the maternalembryofetal vascular circulation. The angiopoietin/TIE pathway is involved in vascular morphogenesis through regulation, survival and maturation of endothelial cells concomitant with vascular remodeling. Deregulation of pro-angiogenic factor secretion and expression is associated with disruption of vascular morphogenesis, reduction of vascular bed and installation of primary placental insufficiency. The aim: Evaluation of Ang 1 immunoexpression in early term compromised pregnancies in the context of chorio-villary circulatory dysfunction in primary placental insufficiency. Material and methods: Abortion product from 61 patients (stagnant pregnancies – 39 cases, early miscarriage – 8 cases, control group – 14 cases of pregnancies solved on social indications/ desire) were immunohistochemically evaluated with the marker for anti-Ang1 and anti-CD31. Results: The villous syncytiotrophoblast was the most immunoreactive area. Most of cases of the pregnancies terminated on social indications/ desire were anti-Ang1 negative. The levels of anti-Ang1 immunoexpression were statistically significantly different in case of syncytiotrophoblast of early miscarriages and abortions terminated on social indications/ desire. The highest chorio-villous vascular density was noticed in the abortions on social indications/ desire and early misscariages group. Conclusions: The placental period is characterized by a weak angiogenic Ang1 differentiated cellular environment in the chorio-villous germinal site in the group of short term compromised pregnancies. The selectively immunoexpressed cellular profile statistically significantly correlates with placental vascular index and chorio-villous vascular density in stagnant pregnancies.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":"47 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139189858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.52418/moldovan-med-j.66-2.23.02
Elena Buga, Eugen Buga, D. Uncuta
Background: One of the most important steps in endodontic treatment is the preparation of a uniform space, the use of endodontic irrigant and creating space for the endodontic filling. Therefore, improving endodontic treatment techniques is one of the most important tasks of modern dentistry. The success and effectiveness of endodontic treatment is largely determined by the quality of chemo-mechanical root canal treatment. In recent years there has been a major emphasis on the use of rotary mechanical instrumentation systems, namely the use of nickel-titanium (NiTi) alloys, which due to their properties represent a favorable flexibility in the instrumentation of difficult anatomies. Material and methods: 20 patients were examined and treated. They were divided into 2 groups: for the treatment of 6 patients was applied the Protaper Universal rotary system. In the second group of 14 patients to whom mechanical instrumentation was applied, were 7 patients following the Protaper Next and the other 7 Dc Taper with different pulpal and periodontal diseases. Results: Mechanical instrumentation was performed on different nosological entities, the prevalence being acute pulpitis. Among instrumented teeth, the prevalence was on the side of pluriradicular teeth. Conclusions: The practical application of different NiTi rotary systems has to be determined individually, as each clinical case has its own practical properties. But the success of an endodontic treatment depends not only on the rotary instruments used and, on the method, chosen, but also on the practitioner’s experience, detailed knowledge of the properties and step-by-step instrumentation protocols.
{"title":"Modern nickel-titanium rotary systems in endodontic treatment","authors":"Elena Buga, Eugen Buga, D. Uncuta","doi":"10.52418/moldovan-med-j.66-2.23.02","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.66-2.23.02","url":null,"abstract":"Background: One of the most important steps in endodontic treatment is the preparation of a uniform space, the use of endodontic irrigant and creating space for the endodontic filling. Therefore, improving endodontic treatment techniques is one of the most important tasks of modern dentistry. The success and effectiveness of endodontic treatment is largely determined by the quality of chemo-mechanical root canal treatment. In recent years there has been a major emphasis on the use of rotary mechanical instrumentation systems, namely the use of nickel-titanium (NiTi) alloys, which due to their properties represent a favorable flexibility in the instrumentation of difficult anatomies. Material and methods: 20 patients were examined and treated. They were divided into 2 groups: for the treatment of 6 patients was applied the Protaper Universal rotary system. In the second group of 14 patients to whom mechanical instrumentation was applied, were 7 patients following the Protaper Next and the other 7 Dc Taper with different pulpal and periodontal diseases. Results: Mechanical instrumentation was performed on different nosological entities, the prevalence being acute pulpitis. Among instrumented teeth, the prevalence was on the side of pluriradicular teeth. Conclusions: The practical application of different NiTi rotary systems has to be determined individually, as each clinical case has its own practical properties. But the success of an endodontic treatment depends not only on the rotary instruments used and, on the method, chosen, but also on the practitioner’s experience, detailed knowledge of the properties and step-by-step instrumentation protocols.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":"13 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139192105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.52418/moldovan-med-j.66-2.23.11
Ecaterina Carpenco, V. Fulga, L. Saptefrati, L. Sinitina, Valeriu David
Background: The angiopoietin/TIE system is one of the signalling pathways that regulate vascular development and remodelling during morphogenesis of the placental complex. Deregulation of the vascularization process is associated with primary placental insufficiency through destabilization of the maternal-fetal functional system. Disruption of expression of pro-angiogenic factors is associated with reduction of the microcirculatory bed and installation of circulatory dysfunction. The aim: Evaluation of Ang2 immunoexpression in early term compromised uterine pregnancies in the context of chorio-villous circulatory dysfunction in primary placental insufficiency. Material and methods: Abortion product from 67 patients with early term compromised pregnancies (stagnant pregnancies – 43 cases, early miscarriages – 11 cases, control batch that included 13 cases of pregnancies solved on social indications) were immunohistochemically evaluated with the marker for anti-Ang2 and anti-CD31. Results: The majority of sites analyzed were Ang2 negative, with the exception of syncytiotrophoblast, that reached the highest score (+3) in most cases. A maximum placental vascularisation index was achieved in the control group. The stagnant pregnacies batch has registered the lowest vascular density mean. Conclusions: The placentation period is characterized by a weak Ang2 cellular environment in the chorio-villous germinal site in the group of short-term compromised pregnancies, except a highly positive syncytiotrophoblast. The immunoexpression profile in vascular endothelium correlates statistically significantly with placental vascularization index and chorio-villous vascular density in stagnant pregnancies.
{"title":"Ang2 immunoexpression vs vascular profile in chorio-villous germinative status in early term compromised pregnancies","authors":"Ecaterina Carpenco, V. Fulga, L. Saptefrati, L. Sinitina, Valeriu David","doi":"10.52418/moldovan-med-j.66-2.23.11","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.66-2.23.11","url":null,"abstract":"Background: The angiopoietin/TIE system is one of the signalling pathways that regulate vascular development and remodelling during morphogenesis of the placental complex. Deregulation of the vascularization process is associated with primary placental insufficiency through destabilization of the maternal-fetal functional system. Disruption of expression of pro-angiogenic factors is associated with reduction of the microcirculatory bed and installation of circulatory dysfunction. The aim: Evaluation of Ang2 immunoexpression in early term compromised uterine pregnancies in the context of chorio-villous circulatory dysfunction in primary placental insufficiency. Material and methods: Abortion product from 67 patients with early term compromised pregnancies (stagnant pregnancies – 43 cases, early miscarriages – 11 cases, control batch that included 13 cases of pregnancies solved on social indications) were immunohistochemically evaluated with the marker for anti-Ang2 and anti-CD31. Results: The majority of sites analyzed were Ang2 negative, with the exception of syncytiotrophoblast, that reached the highest score (+3) in most cases. A maximum placental vascularisation index was achieved in the control group. The stagnant pregnacies batch has registered the lowest vascular density mean. Conclusions: The placentation period is characterized by a weak Ang2 cellular environment in the chorio-villous germinal site in the group of short-term compromised pregnancies, except a highly positive syncytiotrophoblast. The immunoexpression profile in vascular endothelium correlates statistically significantly with placental vascularization index and chorio-villous vascular density in stagnant pregnancies.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":"104 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139195491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.52418/moldovan-med-j.66-2.23.14
S. Odobescu, O. Grosu, Lilia Rotaru, V. Vovc, Ion Moldovanu
Background: The Functional Neurology Research Unit (FNRU) was created in 1992 at the State University of Medicine and Pharmacy of the Republic of Moldova. The activity of the scientific unit was essentially based on the concept of Functional Neurology, which was published in an article in the prestigious Italian journal Functional Neurology (1998) and continues to be further developed by Professors I. Moldovanu and V. Vovc. Conclusions: Physiological aspects in the researches carried out by collaborators of the FNRU concerned research of breathing pattern, autonomic parameters of suprasegmental and segmental level (research of heart rate variability, segmental vegetative samples, etc.), reactivity of cerebral vessels to modeled hypocapnia, trigeminal, somesthetic, visual, auditory evoked potentials in patients with chronic headache, etc. To conclude, the scientific objectives of the research carried out by the collaborators of the Functional Neurology Research Unit during 30 years of activity – the development of the problem of identifying and using the huge, so far still insufficiently researched possibilities of the “reserves” of the human brain for therapeutic purposes (treatment of chronic pain, movement disorders as a result of organic brain lesions, autonomic disorders, etc.) in the context of a new, modern and advanced understanding of the relationship and interaction of the human brain, psyche and body within a social background which is the essence of the functional neurology.
背景：功能神经学研究组（FNRU）于 1992 年在摩尔多瓦共和国国立医药大学成立。该科研小组的活动主要基于功能神经学的概念，该概念发表在意大利著名杂志《功能神经学》（1998 年）上，并由 I. Moldovanu 教授和 V. Vovc 教授继续进一步发展。结论：由 FNRU 的合作者开展的研究中的生理方面涉及呼吸模式研究、上节段和节段水平的自律神经参数（心率变异性研究、节段植物样本等）、脑血管对模拟低碳酸血症的反应、慢性头痛患者的三叉神经、躯体感觉、视觉、听觉诱发电位等。总之，功能神经学研究室的合作者们在 30 年的活动中所开展的研究的科学目标是：在对人脑、心理和身体在社会背景下的关系和相互作用有了新的、现代的和先进的理解的背景下，发现和利用人脑 "储备 "的巨大的、迄今仍未得到充分研究的可能性来达到治疗目的（治疗慢性疼痛、脑器质性病变引起的运动障碍、自律神经失调等），而这正是功能神经学的精髓所在。
{"title":"History of the creation and activity of the Functional Neurology Research Unit over 30 years","authors":"S. Odobescu, O. Grosu, Lilia Rotaru, V. Vovc, Ion Moldovanu","doi":"10.52418/moldovan-med-j.66-2.23.14","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.66-2.23.14","url":null,"abstract":"Background: The Functional Neurology Research Unit (FNRU) was created in 1992 at the State University of Medicine and Pharmacy of the Republic of Moldova. The activity of the scientific unit was essentially based on the concept of Functional Neurology, which was published in an article in the prestigious Italian journal Functional Neurology (1998) and continues to be further developed by Professors I. Moldovanu and V. Vovc. Conclusions: Physiological aspects in the researches carried out by collaborators of the FNRU concerned research of breathing pattern, autonomic parameters of suprasegmental and segmental level (research of heart rate variability, segmental vegetative samples, etc.), reactivity of cerebral vessels to modeled hypocapnia, trigeminal, somesthetic, visual, auditory evoked potentials in patients with chronic headache, etc. To conclude, the scientific objectives of the research carried out by the collaborators of the Functional Neurology Research Unit during 30 years of activity – the development of the problem of identifying and using the huge, so far still insufficiently researched possibilities of the “reserves” of the human brain for therapeutic purposes (treatment of chronic pain, movement disorders as a result of organic brain lesions, autonomic disorders, etc.) in the context of a new, modern and advanced understanding of the relationship and interaction of the human brain, psyche and body within a social background which is the essence of the functional neurology.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":"21 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139194209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The aim of the study was to breakdown the relationship and its nature between the two pathological entities arterial hypertension (AHT) and SARS-CoV-2 infection. The analysis of existing literature on both topics has been conducted. Sources including journals, books, existing publications and online platforms like Google Academic, PubMed, HINARI were used as search engines. Additionally, guidelines and circulars from European Society of Cardiology, American Heart Association and other respective bodies were also referred to. Data from large meta-analyses and clinical studies were included to bring out the relationship study. Pathophysiological breakdown of the two entities, contradicting proposals regarding treatment, new treatment modalities and de novo onset of AHT in post-Covid infection were included to delineate the relationship between the two pathological entities. Conclusions: Findings emphasize that the role of arterial hypertension in SARS-CoV-2 infection is mediated through its effect on the regulation of Renin Angiotensin Aldosteron System (RAAS), inflammation and immune responses. De-novo arterial hypertension was also reported in post SARS-CoV-2 infection patients. Though some initial studies hypothesized that RAAS inhibitors may add to clinical adversities, most studies afterward disproved the same and in fact revealed a protective role of the same. Angiotensin Converting Enzyme 2 (ACE2) is proposed as a treatment option in SARS-CoV-2 infection.
{"title":"Covid-19 infection and arterial hypertension: the relationship between the two entities","authors":"Svetlana Gavriliuc, Abhinand Sanalkumar, Sneha Jeevan, Ghenadii Calin, Diana Sasu","doi":"10.52418/moldovan-med-j.66-2.23.12","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.66-2.23.12","url":null,"abstract":"Background: The aim of the study was to breakdown the relationship and its nature between the two pathological entities arterial hypertension (AHT) and SARS-CoV-2 infection. The analysis of existing literature on both topics has been conducted. Sources including journals, books, existing publications and online platforms like Google Academic, PubMed, HINARI were used as search engines. Additionally, guidelines and circulars from European Society of Cardiology, American Heart Association and other respective bodies were also referred to. Data from large meta-analyses and clinical studies were included to bring out the relationship study. Pathophysiological breakdown of the two entities, contradicting proposals regarding treatment, new treatment modalities and de novo onset of AHT in post-Covid infection were included to delineate the relationship between the two pathological entities. Conclusions: Findings emphasize that the role of arterial hypertension in SARS-CoV-2 infection is mediated through its effect on the regulation of Renin Angiotensin Aldosteron System (RAAS), inflammation and immune responses. De-novo arterial hypertension was also reported in post SARS-CoV-2 infection patients. Though some initial studies hypothesized that RAAS inhibitors may add to clinical adversities, most studies afterward disproved the same and in fact revealed a protective role of the same. Angiotensin Converting Enzyme 2 (ACE2) is proposed as a treatment option in SARS-CoV-2 infection.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":"48 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52418/moldovan-med-j.65-2.22.08
Ivan Civirjic, Serghei Sandru, O. Arnaut, Natalia Cernei, Victoria Moghildea
Background: Analysis and evaluation of the multitude of parameters that impact and mirror clinical evolution of COVID-19 infection. Narrative literature review type of study. Bibliographic search of the PubMed database, applying the keywords: ”SARS-CoV-2”, ”COVID-19”, ”risk score”, ”laboratory parameters”, ”pathophysiology”, ”cytokine storm”, ”imaging evaluation”, “outcomes”, “clinical evolution”, which were combined with each other. There were selected English-language publications, in extenso, published in recognized journals from March 2020. Priority in the analysis was given to articles of critical synthesis of literature, randomized studies, those with large samples of patients. One of the clinically important symptoms that reflects severe or critical clinical evolution is persistent fever during the time. The presence of comorbidities, especially associated with obesity, represents a high risk of severe evolution. Proinflammatory, prothrombotic and systemic endothelial damage processes are represented by changes in platelet count, lymphocytes, neutrophil / lymphocyte ratio, C-reactive protein, D-dimers, fibrinogen, procalcitonin, urea, creatinine, ALS, AST, interleukin-6 and serum ferritin. Bacterial and fungal infections negatively influence clinical evolution. Common prediction scores have low value in COVID-19 patients and need adaptation. Imaging evaluation identifies the type of lung injury and correlates with the severity degree and outcome. Conclusions: COVID-19 disease caused by SARS-CoV-2 virus includes a multitude of pathophysiological changes that through its mechanism represent a systemic nosology. The complete analysis of all the factors and parameters that can influence its clinical evolution is a basic component of the decision-making steps and treatment approach.
{"title":"Factors to consider when assessing the severity of COVID-19","authors":"Ivan Civirjic, Serghei Sandru, O. Arnaut, Natalia Cernei, Victoria Moghildea","doi":"10.52418/moldovan-med-j.65-2.22.08","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.65-2.22.08","url":null,"abstract":"Background: Analysis and evaluation of the multitude of parameters that impact and mirror clinical evolution of COVID-19 infection. Narrative literature review type of study. Bibliographic search of the PubMed database, applying the keywords: ”SARS-CoV-2”, ”COVID-19”, ”risk score”, ”laboratory parameters”, ”pathophysiology”, ”cytokine storm”, ”imaging evaluation”, “outcomes”, “clinical evolution”, which were combined with each other. There were selected English-language publications, in extenso, published in recognized journals from March 2020. Priority in the analysis was given to articles of critical synthesis of literature, randomized studies, those with large samples of patients. One of the clinically important symptoms that reflects severe or critical clinical evolution is persistent fever during the time. The presence of comorbidities, especially associated with obesity, represents a high risk of severe evolution. Proinflammatory, prothrombotic and systemic endothelial damage processes are represented by changes in platelet count, lymphocytes, neutrophil / lymphocyte ratio, C-reactive protein, D-dimers, fibrinogen, procalcitonin, urea, creatinine, ALS, AST, interleukin-6 and serum ferritin. Bacterial and fungal infections negatively influence clinical evolution. Common prediction scores have low value in COVID-19 patients and need adaptation. Imaging evaluation identifies the type of lung injury and correlates with the severity degree and outcome. Conclusions: COVID-19 disease caused by SARS-CoV-2 virus includes a multitude of pathophysiological changes that through its mechanism represent a systemic nosology. The complete analysis of all the factors and parameters that can influence its clinical evolution is a basic component of the decision-making steps and treatment approach.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48919563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52418/moldovan-med-j.65-2.22.01
Valeriu David, V. Petrovici, L. Saptefrati, V. Fulga, Ecaterina Carpenco, Elena Frant
Background: The molar and non-molar lesions are determined during histomorphological examination and treated as inconclusive. The establishing of a marker by immunohistochemical investigations could influence the accuracy of the morphopathological diagnosis. The aim is evaluation of p57 immunoexpression in the trophoblastic germ compartment in molar and non-molar pregnancies. Material and methods: Abortion products from 15 patients with hydatidiform mole, 18 pregnancies solved on social indications and 16 short-term disordered pregnancies were evaluated depending on the immunoexpresion of p57. Results: The hydatidiform mole was classified based on anti-p57 immunoexpression into: complete hydatidiform mole – 8 cases (negative immunoexpression or expression in ˂10% of villous cytotrophoblast) and partial hydatidiform mole – 7 cases (positive expression in ˃10% of the villous cytotrophoblast). Basal deciduous and extravillous cytotrophoblasts were positive in 100% of cases and served as internal control. Hepatocytes were used as negative control. In the control group, the positive immunoexpression was attested in >10% of cases in the villous trophoblast. Conclusions: Differential immunoexpression of p57 protein in the germinal cytotrophoblast allows subclassification of molar pathology into complete and partial forms, while not allowing the differentiation between partial hydatidiform mole and non-molar lesions. Immunohistochemical evaluation of p57kip2 protein in molar and non-molar pathology is useful in differential diagnosis as a complementary method.
{"title":"Evaluation of p57 expression in early disordered pregnancies with molar status vs non-molar ones","authors":"Valeriu David, V. Petrovici, L. Saptefrati, V. Fulga, Ecaterina Carpenco, Elena Frant","doi":"10.52418/moldovan-med-j.65-2.22.01","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.65-2.22.01","url":null,"abstract":"Background: The molar and non-molar lesions are determined during histomorphological examination and treated as inconclusive. The establishing of a marker by immunohistochemical investigations could influence the accuracy of the morphopathological diagnosis. The aim is evaluation of p57 immunoexpression in the trophoblastic germ compartment in molar and non-molar pregnancies. Material and methods: Abortion products from 15 patients with hydatidiform mole, 18 pregnancies solved on social indications and 16 short-term disordered pregnancies were evaluated depending on the immunoexpresion of p57. Results: The hydatidiform mole was classified based on anti-p57 immunoexpression into: complete hydatidiform mole – 8 cases (negative immunoexpression or expression in ˂10% of villous cytotrophoblast) and partial hydatidiform mole – 7 cases (positive expression in ˃10% of the villous cytotrophoblast). Basal deciduous and extravillous cytotrophoblasts were positive in 100% of cases and served as internal control. Hepatocytes were used as negative control. In the control group, the positive immunoexpression was attested in >10% of cases in the villous trophoblast. Conclusions: Differential immunoexpression of p57 protein in the germinal cytotrophoblast allows subclassification of molar pathology into complete and partial forms, while not allowing the differentiation between partial hydatidiform mole and non-molar lesions. Immunohistochemical evaluation of p57kip2 protein in molar and non-molar pathology is useful in differential diagnosis as a complementary method.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48696516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52418/moldovan-med-j.65-2.22.05
O. Macagonova, Adrian Cociug, T. Braniste, V. Nacu
Introduction: Decellularized biomaterials derived from the biological tissues are ideal for tissue engineering applications because they mimic the biochemical composition of the native tissue. The physical and structural properties of the scaffold are important in the fields of tissue engineering and regenerative medicine. Material and methods: Study material was 20 decellularized dermal grafts. 10 samples were obtained from piglets slaughtered in the slaughterhouse. Other tissues (n=10) were received from the donor from the Human Tissue and Cell Bank of the Republic of Moldova. Extracellular matrices were obtained by decellularization with 0.5% sodium dodecyl sulfate/0.1% EDTA solution. The evaluation of the structural characteristics was carried out by the histological examination with hematoxylin and eosin, scanning electron microscopy and the quantification of the amount of deoxyribonucleic acids. Assessment of the physical characteristics included analysis of extracellular matrix volume porosity, density, and swelling rate. Results: Histological examination revealed fewer cells in decellularized tissues compared to non-decellularized ones. More than 80.5% of nucleic acids were removed from porcine matrix and 82.5% of genetic material – from decellularized human dermal structures. A mean correlation and inverse dependence of -0.43 was shown between porosity and swelling rate of decellularized dermis. Conclusions: The decellularization process significantly (P<0.05) removed the cellular components while preserving the connective three-dimensional structure of the dermal matrices clearly shown by quantification of the amount of DNA and microscopic examination of the structures.
{"title":"Structural and physical characteristics of the dermal decellularized structures evaluation","authors":"O. Macagonova, Adrian Cociug, T. Braniste, V. Nacu","doi":"10.52418/moldovan-med-j.65-2.22.05","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.65-2.22.05","url":null,"abstract":"Introduction: Decellularized biomaterials derived from the biological tissues are ideal for tissue engineering applications because they mimic the biochemical composition of the native tissue. The physical and structural properties of the scaffold are important in the fields of tissue engineering and regenerative medicine. Material and methods: Study material was 20 decellularized dermal grafts. 10 samples were obtained from piglets slaughtered in the slaughterhouse. Other tissues (n=10) were received from the donor from the Human Tissue and Cell Bank of the Republic of Moldova. Extracellular matrices were obtained by decellularization with 0.5% sodium dodecyl sulfate/0.1% EDTA solution. The evaluation of the structural characteristics was carried out by the histological examination with hematoxylin and eosin, scanning electron microscopy and the quantification of the amount of deoxyribonucleic acids. Assessment of the physical characteristics included analysis of extracellular matrix volume porosity, density, and swelling rate. Results: Histological examination revealed fewer cells in decellularized tissues compared to non-decellularized ones. More than 80.5% of nucleic acids were removed from porcine matrix and 82.5% of genetic material – from decellularized human dermal structures. A mean correlation and inverse dependence of -0.43 was shown between porosity and swelling rate of decellularized dermis. Conclusions: The decellularization process significantly (P<0.05) removed the cellular components while preserving the connective three-dimensional structure of the dermal matrices clearly shown by quantification of the amount of DNA and microscopic examination of the structures.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43404107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52418/moldovan-med-j.65-2.22.04
O. Ignatov, Adrian Cociug, O. Pascal, V. Nacu
Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest biomaterials used for scaffolds. Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed. Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24 hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete. Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results.
{"title":"Morphological evaluation of the amniotic membrane decellularization","authors":"O. Ignatov, Adrian Cociug, O. Pascal, V. Nacu","doi":"10.52418/moldovan-med-j.65-2.22.04","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.65-2.22.04","url":null,"abstract":"Background: Biological materials derived from decellularized tissues could be a good basis for progress in regenerative medicine while maintaining the main components of the extracellular matrix. A promising scaffold for tissue-engineered is the human amniotic membrane. It is one of the oldest biomaterials used for scaffolds. Material and methods: 3 placentas were obtained through Human Tissue Bank. Under sterile condition human amniotic membrane was collected. The human amniotic membrane was treated with 0.5% of sodium dodecyl sulphate (SDS), 1% Triton for 24 and 5 hours. Amniotic membrane decellularization was also carried out in combination with ultrasound bath for 20 minutes 3 times. For morphological and structure evaluation of human amniotic membrane the scanning electron microscopy of native amniotic membrane and histology of decellularized and native amniotic membrane were performed. Results: The human amniotic membrane decellularization process with 0.5% SDS solution and 1% Triton solution showed that decellularization for 24 hours is too aggressive for human amniotic membrane structure. The decellularization for 5h with 1% Triton solution was incomplete. Conclusions: The method of decellularization with 0.5% SDS solution is more suitable for amniotic membrane decellularization and can be performed in only 5 hours. The use of ultrasound bath did not have a significant effect on the obtained results.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48499566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.52418/moldovan-med-j.65-2.22.02
Ecaterina Carpenco, V. Petrovici, L. Sinitina, V. Fulga, Valeriu David
Background: The hydatiform mole is charcaterized by a pathological proliferation of the trophoblast. Its evaluation could predict the progression to gestational trophoblastic neoplasia. The aim of the study was the analysis of the proliferative activity of the villous trophoblast in the molar vs non-molar lesions. Material and methods: p-57 and Ki-67 were evaluated by immunohistochemistry in 15 cases of hydatidiform mole and 18 cases of abortions. Results: The hydatidiform mole was divided based on the immunoexpression of anti-p57 into: complete (8 cases) and partial type (7 cases). The distribution score of Ki67 immunoreactivity in the villous cytotrophoblast was: complete mole: +3 – 8 cases; partial mole: +1 – 1 case, +2 – 3 cases, +3 – 3 cases; abortions on social indications: +1 – 6 cases, +2 – 9 cases; +3 – 2 cases. The mean and standard deviations were: 2.88±0.354; 2.29±0.756 and 1.82±0.728, respectively. The following statistical correlations were determined: complete vs partial mole (p=0.014), complete mole vs abortion (p<0.01) and overall cases of mole vs abortion (p=0.000034). Conclusions: Villous cytotrophoblast proliferative activity is high in the complete hydatidiform mole, and the immunoreactivity distribution index is highly positive and statistically true in the molar versus non-molar group. Immunohistochemical evaluation of Ki-67 in molar pathology is useful in differential diagnosis as a complementary method.
{"title":"The expression of Ki-67 marker in the hydatidiform mole","authors":"Ecaterina Carpenco, V. Petrovici, L. Sinitina, V. Fulga, Valeriu David","doi":"10.52418/moldovan-med-j.65-2.22.02","DOIUrl":"https://doi.org/10.52418/moldovan-med-j.65-2.22.02","url":null,"abstract":"Background: The hydatiform mole is charcaterized by a pathological proliferation of the trophoblast. Its evaluation could predict the progression to gestational trophoblastic neoplasia. The aim of the study was the analysis of the proliferative activity of the villous trophoblast in the molar vs non-molar lesions. Material and methods: p-57 and Ki-67 were evaluated by immunohistochemistry in 15 cases of hydatidiform mole and 18 cases of abortions. Results: The hydatidiform mole was divided based on the immunoexpression of anti-p57 into: complete (8 cases) and partial type (7 cases). The distribution score of Ki67 immunoreactivity in the villous cytotrophoblast was: complete mole: +3 – 8 cases; partial mole: +1 – 1 case, +2 – 3 cases, +3 – 3 cases; abortions on social indications: +1 – 6 cases, +2 – 9 cases; +3 – 2 cases. The mean and standard deviations were: 2.88±0.354; 2.29±0.756 and 1.82±0.728, respectively. The following statistical correlations were determined: complete vs partial mole (p=0.014), complete mole vs abortion (p<0.01) and overall cases of mole vs abortion (p=0.000034). Conclusions: Villous cytotrophoblast proliferative activity is high in the complete hydatidiform mole, and the immunoreactivity distribution index is highly positive and statistically true in the molar versus non-molar group. Immunohistochemical evaluation of Ki-67 in molar pathology is useful in differential diagnosis as a complementary method.","PeriodicalId":32733,"journal":{"name":"The Moldovan Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49527002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：