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The impact of pressure-shift freezing on the three-dimensional network structure and properties of myofibrillar proteins in surimi gel derived from freshwater fish 变压冷冻对淡水鱼鱼糜凝胶中肌纤维蛋白三维网络结构和特性的影响
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-18 DOI: 10.1016/j.ifset.2024.103673
Sinan Zhang , Maninder Meenu , Ting Xiao , Lihui Hu , Junde Ren , Hosahalli S. Ramaswamy , Yong Yu

Freezing treatment can prolong the shelf life of surimi products, but it will lead to deterioration of surimi gel quality. This study compared the effects of three different processing methods — non-freezing processing (control), conventional air freezing (CAF), and pressure-shift freezing (PSF) — on the three-dimensional (3D) network structure of grass carp surimi gel. After PSF treatment, the pores in the 3D gel network structure were denser compared to the control group which facilitates interaction of water and network structure. Furthermore, the ultraviolet and fluorescence spectral analysis indicated that PSF treatment mitigates the decline in stability of the tertiary structure of myofibrillar protein (MP) during freezing. The microstructure of MP showed that PSF treatment promotes the cross-linking of MP to form protein aggregates and then promotes the gelation of MP.

Industrial relevance

PSF can increase the commodity value of low-value freshwater fish products and has potential in the development of new products of surimi gels. This study reported a novel idea and theoretical basis for the application of PSF technology in the frozen food and aquatic product processing industry.

冷冻处理可以延长鱼糜产品的保质期,但会导致鱼糜凝胶质量下降。本研究比较了非冷冻处理(对照组)、传统空气冷冻(CAF)和移压冷冻(PSF)三种不同处理方法对草鱼鱼糜凝胶三维网络结构的影响。经 PSF 处理后,三维凝胶网络结构中的孔隙比对照组更密集,这有利于水与网络结构的相互作用。此外,紫外光谱和荧光光谱分析表明,PSF 处理可减轻冷冻过程中肌纤维蛋白三级结构稳定性的下降。MP的微观结构表明,PSF处理可促进MP交联形成蛋白质聚集体,进而促进MP的凝胶化。工业相关性PSF可提高低值淡水鱼产品的商品价值,并具有开发鱼糜凝胶新产品的潜力。本研究为 PSF 技术在冷冻食品和水产品加工业中的应用提供了新的思路和理论依据。
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引用次数: 0
Orally self-disintegrating milk protein-based puffs enriched with in-process generated short chain galacto-oligosaccharides by supercritical fluid extrusion 通过超临界流体挤压法在以牛奶蛋白为基础的口腔自崩解泡芙中添加加工过程中产生的短链半乳糖-低聚糖
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-18 DOI: 10.1016/j.ifset.2024.103667
Jessica A. Uhrin, Syed S.H. Rizvi

This study aimed to design and evaluate orally self-disintegrating puffs that are nutritionally enhanced via in-process polymerization of galactose into galacto-oligosaccharides (GOS). Lactose hydrolyzed (LHSMP) or unhydrolyzed skim milk powder (SMP) was combined with 80% protein milk protein concentrate (MPC) powder and a calcium chelator, then processed using supercritical fluid extrusion (SCFX) with carbon dioxide (CO2) to produce protein-rich orally self-disintegrating puffs. Disintegration results indicate the MPC90 and MPC70-SMP20 puffs had faster disintegration rates (3.20 and 3.84%/sec) than the commercial puffs (3.12%/sec). Galactose in LHSMP as a precursor to formation of polymeric GOS during extrusion was also investigated. The low pH and controlled temperature minimize Maillard browning and enhanced the polymerization of galactose into GOS. The MPC60-LHSMP30 puffs had the highest content of short chain GOS (2.3 wt%). The current study provides first valuable insights for the utilization of LHSMP to produce orally self-disintegrating GOS enriched protein puffs.

本研究旨在设计和评估通过在加工过程中将半乳糖聚合成半乳寡糖(GOS)来增强营养的口服自溶泡芙。将水解乳糖(LHSMP)或未水解脱脂奶粉(SMP)与 80% 蛋白质含量的浓缩牛奶蛋白(MPC)粉和钙螯合剂混合,然后使用二氧化碳(CO2)超临界流体挤压(SCFX)工艺加工,生产出富含蛋白质的口服自崩解泡芙。崩解结果表明,MPC90 和 MPC70-SMP20 泡芙的崩解率(3.20%/秒和 3.84%/秒)高于商用泡芙(3.12%/秒)。此外,还对 LHSMP 中的半乳糖作为挤压过程中形成聚合物 GOS 的前体进行了研究。低 pH 值和受控温度最大程度地减少了马氏褐变,并促进了半乳糖聚合成 GOS。MPC60-LHSMP30 膨化食品的短链 GOS 含量最高(2.3 wt%)。目前的研究首次为利用 LHSMP 生产口服自崩解 GOS 富集蛋白泡芙提供了宝贵的见解。
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引用次数: 0
Separation of milk fat using silicon carbide support ceramic membranes with different pore sizes 使用不同孔径的碳化硅支撑陶瓷膜分离牛奶脂肪
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-17 DOI: 10.1016/j.ifset.2024.103671
Tobias Dons , Victor Candelario , Ulf Andersen , Lilia Ahrné

The effect of pore size and type of material on the separation of fat from raw milk using hydrophilic silicon carbide (SiC) support ceramic membranes has not previously been reported in literature. The separation performance of the fat globules (MFGs) showed 98% for SiC 0.5 μm, 92% for SiC 1.4 μm, 90% fat for ZrO2-SiC 0.06 μm, while the permeate had a fat % ranging from 0.1 to 0.6% (w/w). The MFGs were kept highly intact with well distributed proteins and phospholipids in the MFG membrane. The total filtration time at 50 °C to reach maximum VCR was 134 min for ZrO2 (VCR 3), 148 min for SiC 0.5 μm and 16 min for SiC 1.4 μm (VCR4). Further, all membranes showed a fully recoverable water permeability indicating no irreversible fouling.

Industrial relevance

Microfiltration may be an alternative process to fat separation from raw milk. In this study, for the first time was demonstrated that effective milk fat separation, and retentate and permeate streams with variable characteristics, in terms of composition and integrity of the milk fat globules can be obtained by using silicon carbide support membranes with different sizes and materials. The results obtained, provide new insights for industrial use of membrane technology to separate milk.

使用亲水性碳化硅(SiC)支撑陶瓷膜分离原料奶中的脂肪时,孔径大小和材料类型对其分离效果的影响此前尚未见文献报道。碳化硅 0.5 μm 的脂肪球(MFGs)分离率为 98%,碳化硅 1.4 μm 的分离率为 92%,ZrO2-碳化硅 0.06 μm 的脂肪分离率为 90%,而渗透物的脂肪含量为 0.1% 至 0.6%(重量比)。MFG 保持高度完整,蛋白质和磷脂在 MFG 膜上分布均匀。在 50 °C 条件下,达到最大 VCR 的总过滤时间分别为:ZrO2 134 分钟(VCR 3)、SiC 0.5 μm 148 分钟和 SiC 1.4 μm 16 分钟(VCR 4)。此外,所有膜都显示出完全可恢复的透水性,表明没有不可逆的污垢。这项研究首次证明,使用不同尺寸和材料的碳化硅支撑膜可以有效分离牛奶脂肪,并获得在牛奶脂肪球的组成和完整性方面具有不同特性的回流液和渗透液。所获得的结果为工业上使用膜技术分离牛奶提供了新的思路。
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引用次数: 0
Photothermal pasteurization of paprika powder by ultra-high irradiance blue (405 nm) light: Impact on Salmonella inactivation and quality attributes 用超高辐照度蓝光(405 纳米)对辣椒粉进行光热杀菌:对沙门氏菌灭活和质量属性的影响
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-16 DOI: 10.1016/j.ifset.2024.103672
Martha Minor , Sergio I. Martínez-Monteagudo , Luis Sabillón

This study aimed to evaluate the inactivation efficacy of ultra-high irradiance blue (405 nm) LED treatments against Salmonella spp. in paprika powder and develop an alternative Weibull model to describe the inactivation kinetics. Paprika samples were inoculated with a Salmonella cocktail at a ratio of 1 mL/100 g (∼8.7 log CFU/g), acclimated for 7 days at 22 °C, and irradiated with blue LED at a fixed distance (5 cm) using different irradiances (548, 697, and 842 mW/cm2) and exposure times (60, 120, 180, and 240 s). Sterilization-like temperatures of up to 131.7 °C were observed in paprika after 240 s at 842 mW/cm2. Reduction levels in Salmonella counts ranged from 2.1 to 7.8 logs after 240 s. A modified Weibull model satisfactorily described the Salmonella inactivation kinetics (Radj2 > 0.98). Compared to control samples, a significant decrease in redness (a* values) and higher DPPH scavenging activity were observed in treated paprika powders.

Industrial relevance: The obtained results indicated that UHI LED technology could serve as an alternative dry pasteurization method to improve the microbiological safety of spices. This would open further possibilities, including the use of this technology to disinfect food-contact surfaces in dry operations. These findings are thus important in supporting the development and optimization of LED technology for food safety applications in the food industry. Blue light has a shorter penetration depth, which may limit the size and shape of products that can be pasteurized with UHI blue LED technology.

本研究旨在评估超高辐照度蓝光(405 nm)LED 处理对辣椒粉中沙门氏菌的灭活效果,并建立一个可供选择的 Weibull 模型来描述灭活动力学。以 1 mL/100 g(∼8.7 log CFU/g)的比例在辣椒粉样品中接种沙门氏菌鸡尾酒,在 22 °C 下驯化 7 天,然后使用不同的辐照度(548、697 和 842 mW/cm2)和照射时间(60、120、180 和 240 s)在固定距离(5 cm)内照射蓝光 LED。在 842 mW/cm2辐照 240 秒后,红辣椒的灭菌温度高达 131.7 °C。240 秒后,沙门氏菌数量减少了 2.1 到 7.8 logs 不等。改良的 Weibull 模型对沙门氏菌灭活动力学进行了满意的描述(Radj2 > 0.98)。与对照样品相比,经处理的辣椒粉的红度(a*值)显著降低,DPPH清除活性更高:研究结果表明,UHI LED 技术可作为干式巴氏杀菌法的替代方法,提高香料的微生物安全性。这将带来更多的可能性,包括在干法操作中使用该技术对与食品接触的表面进行消毒。因此,这些发现对于支持开发和优化 LED 技术在食品工业中的食品安全应用非常重要。蓝光的穿透深度较短,这可能会限制使用超高温蓝光 LED 技术进行巴氏杀菌的产品的尺寸和形状。
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引用次数: 0
Micro-nano bubble water technology: Sustainable solution for the postharvest quality and safety management of fresh fruits and vegetables – A review 微纳米气泡水技术:新鲜水果和蔬菜采后质量与安全管理的可持续解决方案 - 综述
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-15 DOI: 10.1016/j.ifset.2024.103665
Harold K. Malahlela , Zinash A. Belay , Rebogile R. Mphahlele , Oluwafemi James Caleb

Public health concern on carcinogenic residue from chlorine-based disinfectant-by-product on ready-to-eat fresh/fresh-cut produce, and the increase in microbial resistance to chlorine disinfection, has heightened the need for alternative water-based microbial decontaminant. Activated water systems are emerging as alternatives to chlorine-based disinfectants in the fresh/fresh-cut produce industry for the cleaning and decontamination. This review provides a comprehensive description of the micro-nano bubble (MNBs) technology. This includes discussion on variety of existing generation methods of MNBs and the physico-chemical properties of MNBs. The factors affecting MNBs efficacy and stability in solution (such as gas source/type and bubble sizes) were discussed. In addition, recent postharvest applications of MNBs in fruits and vegetables (FVs) and the impact on overall quality attributes were summarized. The need for more MNBs investigations for postharvest management of FFVs quality and safety was highlighted to encourage the scale up and commercial application of the technology in the fresh/fresh-cut produce industry.

公众对即食生鲜/鲜切农产品中氯基消毒剂副产品的致癌残留物的担忧,以及微生物对氯消毒的抗药性的增加,加剧了对替代性水基微生物去污剂的需求。活性水系统作为氯基消毒剂的替代品,正逐渐出现在新鲜/鲜切农产品行业的清洁和去污过程中。本综述全面介绍了微纳米气泡(MNBs)技术。其中包括对现有各种 MNB 生成方法和 MNB 物理化学特性的讨论。还讨论了影响 MNB 在溶液中的功效和稳定性的因素(如气源/类型和气泡大小)。此外,还总结了 MNBs 最近在水果和蔬菜(FVs)中的采后应用及其对整体质量属性的影响。会议强调,需要对采后管理果蔬的质量和安全进行更多的 MNBs 研究,以鼓励在新鲜/鲜切农产品行业扩大该技术的规模和商业应用。
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引用次数: 0
Identification of turntable function in a solid-state microwave heating process with diverse frequency-shifting strategies applied 确定固态微波加热过程中的转盘功能,并采用不同的频率转换策略
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-14 DOI: 10.1016/j.ifset.2024.103670
Ran Yang, Jiajia Chen

The turntable has been used in conventional magnetron-based microwave ovens to improve the heating uniformity. Meanwhile, with the emerging solid-state technique, well-designed frequency-shifting strategies also show promise in achieving improved microwave outcomes without a rotating turntable. This study used modeling tools to comprehensively assess the turntable function under various conditions, where the affecting factors included vertical and horizontal positions of food, rotation status of the turntable, frequency-shifting strategy, and food configurations. Results illustrated that the elevation of food by the turntable is more critical to power efficiency and heating uniformity, while rotation mainly works to narrow the temperature difference between hot and cold spots. Moreover, the complementary-shifting strategy lessened the rotation function of the turntable. Hence, in a solid-state microwave system embedded with a proper frequency-shifting strategy, a simpler supporting component shall replace the current rotating turntable, streamlining the oven design without compromising the microwave performance.

Industrial relevance

This study comprehensively evaluated functions of the turntable, a conventional component, inside a novel microwave system design utilizing solid-state instead of the magnetron as its power source. Based on the evaluation, the well-implemented frequency strategies hold promise to achieve improved microwave performance with a simpler supporting component to elevate the food, rather than relying on a rotating turntable. These findings provide novel insights for designing the next-generation microwave ovens.

传统的磁控微波炉一直使用转盘来改善加热的均匀性。同时,随着固态技术的兴起,精心设计的移频策略也有望在不使用旋转转盘的情况下实现更好的微波效果。本研究利用建模工具全面评估了各种条件下转盘的功能,其中影响因素包括食物的垂直和水平位置、转盘的旋转状态、移频策略和食物配置。结果表明,转盘对食物的抬升对功率效率和加热均匀性更为关键,而旋转则主要起到缩小冷热点温差的作用。此外,互补转换策略减少了转盘的旋转功能。因此,在嵌入了适当移频策略的固态微波系统中,一个更简单的辅助组件将取代目前的旋转转盘,从而简化烤箱设计,同时不影响微波性能。根据评估结果,实施良好的频率策略有望提高微波性能,使用更简单的辅助部件来提升食物,而不是依赖旋转转盘。这些发现为设计下一代微波炉提供了新的见解。
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引用次数: 0
3D printing of rice starch incorporated peanut protein isolate paste: Rheological characterization and simulation of flow properties 大米淀粉与花生蛋白分离物糊状物的 3D 打印:流变表征和流动特性模拟
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-13 DOI: 10.1016/j.ifset.2024.103669
Amaresh Kadival , Jayeeta Mitra , Rajendra Machavaram , Manish Kaushal

This study investigates the viscosity and creep behavior of peanut protein isolate (PPI) paste incorporated with rice starch (RS) for potential applications in 3D printing. The change in flow properties during the 3D printing was also studied by numerical simulation. The paste exhibited shear-thinning behavior with yield stress. The pressure predicted from the Herschel-Bulkley model showed higher values than those derived from the Power-Law model. The PPI and RS levels had negligible influence on velocity and shear rate; however, they showed greater dependence on the inflow rate. The fractional element model described the creep data well (R2 > 0.9). The addition of PPI resulted in increased viscosity, simulated pressure, and mean extrusion force, with similar effects observed for RS, except at higher PPI levels. Furthermore, the 3D-printed objects revealed that deformations were pronounced at low concentrations of PPI and RS, while resolution suffered when employing higher concentrations of PPI and RS.

Industrial relevance

Using peanut protein isolate, a plant-based high protein ingredient in 3D printing, reduces energy consumption, thereby reducing carbon footprints, offers improved functional properties, and diversifies dietary options by offering an alternative protein source from peanuts. The printing is influenced by the rheological properties of food ink. Thus, knowing the rheological properties of food ink can help researchers and industries to prepare food inks with desired properties. The observations of numerical simulation of this work have the potential to offer practical insights for designing 3D printing systems. In summary, this work has the potential to aid in improving and developing food ink for 3D printing, hence enhancing the performance of extrusion-based 3D food printers.

本研究调查了花生蛋白分离物(PPI)糊状物与大米淀粉(RS)的粘度和蠕变行为,以了解其在三维打印中的潜在应用。此外,还通过数值模拟研究了三维打印过程中流动特性的变化。糊状物表现出具有屈服应力的剪切稀化行为。Herschel-Bulkley 模型预测的压力值高于 Power-Law 模型得出的值。PPI 和 RS 水平对速度和剪切速率的影响微乎其微,但对流入速率的依赖性较大。分数元素模型很好地描述了蠕变数据(R2 > 0.9)。添加 PPI 会增加粘度、模拟压力和平均挤出力,除了 PPI 水平较高时,RS 也有类似的效果。此外,3D 打印对象显示,PPI 和 RS 浓度较低时,变形明显,而 PPI 和 RS 浓度较高时,分辨率降低。打印受食品油墨流变特性的影响。因此,了解食品油墨的流变特性有助于研究人员和工业界制备具有所需特性的食品油墨。这项工作的数值模拟观测结果有可能为三维打印系统的设计提供实用见解。总之,这项工作有望帮助改进和开发用于三维打印的食品油墨,从而提高基于挤压的三维食品打印机的性能。
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引用次数: 0
End-over-end (EoE) rotation of toroidal cans: An experimentally validated mathematical modelling study 环形罐的端对端(EoE)旋转:经实验验证的数学建模研究
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-12 DOI: 10.1016/j.ifset.2024.103668
David Alonso de Mezquia , Uxue Arrieta , M. Mounir Bou-Ali , Ozan Altin , Eda Coskun , Trond Løvdal , Dagbjørn Skipnes , Bart Van Droogenbroeck , Fabrizio Sarghini , Ferruh Erdogdu

Toroidal cans have been presented recently to improve the canning process efficiency, and previous studies focused on static and axial rotational processes. End-over-End (EoE) rotation is a significant agitation approach to increase the temperature uniformity of liquid canned products, and it would be important to observe the effect of this process combined with the toroidal cans. Therefore, in this study, this combined effect was considered to improve the process in the view of temperature uniformity. For this purpose, a computational numerical model was developed using - Star CCM+, and this model was validated with experimental data. Then, the rotational rate and headspace amount effects in a toroidal can geometry, including a liquid, were determined using this validated model. The numerical results indicated that these parameters influenced the heat transfer process with a resulting uniform temperature distribution through the EoE processing.

为了提高罐头加工效率,最近出现了环形罐,以前的研究主要集中在静态和轴向旋转工艺上。端-端(EoE)旋转是提高液体罐头产品温度均匀性的一种重要搅拌方法,因此观察这种工艺与环形罐的结合效果非常重要。因此,在本研究中,从温度均匀性的角度考虑了这一组合效果,以改进工艺。为此,使用 - Star CCM+ 开发了一个计算数值模型,并用实验数据对该模型进行了验证。然后,利用该验证模型确定了包括液体在内的环形罐几何形状中的旋转速率和顶空量效应。数值结果表明,这些参数影响了传热过程,从而使整个 EoE 加工过程的温度分布均匀。
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引用次数: 0
Changes in properties of myofibrillar protein and myofibrillar protein gel from freshwater fish after low-temperature and high-pressure collaborative treatment 淡水鱼肌纤维蛋白和肌纤维蛋白凝胶经低温高压协同处理后的性质变化
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-10 DOI: 10.1016/j.ifset.2024.103663
Sinan Zhang , Maninder Meenu , Ting Xiao , Junde Ren , Lihui Hu , Tao Song , Hosahalli S. Ramaswamy , Yong Yu

This study investigated the effects of low-temperature and high-pressure collaborative and conventional air freezing (CAF) on myofibrillar protein (MP) characteristics from grass carp. Turbidity, solubility, and particle size analysis showed that the pressure shift freezing (PSF) treatment could inhibit the formation of large MP aggregates to a certain extent during freezing. Chemical interaction studies unveiled that PSF treatment not only mitigates damage to the inherent structure of MP during freezing but also enhances its crosslinking ability. Microstructure analysis revealed a densely woven fibrous protein network structure within the MP gel in the PSF group. MP gel with fiber shape network structure in the PSF group exhibited stronger water-holding capacity and mechanical properties compared to the CAF group having an incomplete protein network structure. This suggests that the combined application of low-temperature and high-pressure treatment holds promising potential for utilization in the surimi gel processing industry and aquatic products processing.

Industrial relevance: The quality and safety of food are closely related to the commodity value of food. Combined low-temperature and high-pressure treatment can enhance the commodity value of low-value freshwater fish products and has potential in surimi gel new product development. This study provides theoretical guidance for high-pressure and low-temperature collaborative processing and industrial production of related aquatic products.

本研究探讨了低温高压协同冷冻和传统空气冷冻(CAF)对草鱼肌纤蛋白(MP)特性的影响。浊度、溶解度和粒度分析表明,移压冷冻(PSF)处理能在一定程度上抑制冷冻过程中大的 MP 聚集体的形成。化学作用研究表明,PSF 处理不仅能减轻冷冻过程中对 MP 固有结构的破坏,还能增强其交联能力。微观结构分析表明,PSF 组的 MP 凝胶中存在密集编织的纤维状蛋白质网络结构。与蛋白质网络结构不完整的 CAF 组相比,PSF 组具有纤维状网络结构的 MP 凝胶具有更强的保水能力和机械性能。这表明,低温和高压处理的联合应用在鱼糜凝胶加工业和水产品加工中具有广阔的应用前景:食品的质量和安全与食品的商品价值密切相关。低温高压联合处理可提高低值淡水鱼产品的商品价值,在鱼糜凝胶新产品开发方面具有潜力。本研究为相关水产品的高压低温协同处理和工业化生产提供了理论指导。
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引用次数: 0
Stabilization of Pickering emulsions with bacterial cellulose nanofibrils (BCNFs) fabricated by electron beam irradiation 用电子束辐照制造的细菌纤维素纳米纤维(BCNFs)稳定皮克林乳液
IF 6.6 1区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-09 DOI: 10.1016/j.ifset.2024.103664
Yinfeng Tan , Pengrui Wu , Jiangtao Yu , Junqing Bai , Chunling Nie , Bingqian Liu , Yefan Niu , Guangsen Fan , Jianguo Wang

Bacterial cellulose (BC) had been considered as a promising Pickering stabilizer for its non-toxicity, high purity, and biocompatibility. The electron beam irradiation (EBI) technology had gained significant attention in the field of cellulose degradation due to its operational convenience and mild reaction conditions. Pickering emulsions using BC degraded by EBI as Pickering stabilizers were successfully prepared. Irradiated bacterial cellulose nanofibers (IBCNFs) exhibited a high aspect ratio(30–80 μm in length and 21–109 nm in width) and high absolute zeta potential (-50 mV). The optimal emulsification performance of IBCNFs was observed at an irradiation dosage of 100 kGy (IBC-100). The emulsions based on IBC-100 achieved an EI value of 96.15 ± 0.19%, while those based on IBC-500 obtained an EI value of 59.93 ± 1.47%. Stable Pickering emulsions were prepared when the concentration of IBC-100 stabilizer exceeded 0.3 wt% with a fixed oil phase of 30%, or when the rapeseed oil phase was below 50% while maintaining a fixed suspension of 0.5 wt% IBCNFs. Confocal laser scanning microscopy (CLSM) demonstrated that IBCNFs facilitated the formation of a dense interfacial layer at the oil-water interface and formed a three-dimensional network within the water phase. Which effectively hindered the aggregation and flocculation between individual oil droplets. Furthermore, rheological findings demonstrated that emulsions possessed primarily exhibited properties of elasticity, and their gel strength could be enhanced by adjusting the irradiation dosages of IBCNFs. Moreover, environmental stability experiments indicated that IBCNFs-based emulsions exhibited exceedingly high temperature (20–80 °C) and pH tolerance (4–12). Flocculation occurred under high acid (pH = 2) conditions and at high ionic levels (≥ 40 mM NaCl), without causing disruption to the emulsion droplet structure. This study provided an innovative approach to preparing cellulose nanofibers with high aspect ratio, which could be utilized as emulsion stabilizers. The study also demonstrated that Pickering emulsions based on IBCNFs exhibit excellent stability and rheological properties. IBCNFs have promising potential to serve as a natural Pickering emulsifier for stabilizing oil-in-water emulsions in various food applications, cosmetic and pharmaceutical formulations.

细菌纤维素(BC)因其无毒性、高纯度和生物相容性而被认为是一种很有前途的皮克林稳定剂。电子束辐照(EBI)技术因其操作方便、反应条件温和而在纤维素降解领域备受关注。使用经 EBI 降解的 BC 作为 Pickering 稳定剂,成功制备了 Pickering 乳液。辐照细菌纤维素纳米纤维(IBCNFs)具有高纵横比(长 30-80 μm,宽 21-109 nm)和高绝对 Zeta 电位(-50 mV)。在辐照剂量为 100 kGy(IBC-100)时,IBCNFs 的乳化性能最佳。基于 IBC-100 的乳液的 EI 值为 96.15 ± 0.19%,而基于 IBC-500 的乳液的 EI 值为 59.93 ± 1.47%。当 IBC-100 稳定剂的浓度超过 0.3 wt%,固定油相为 30%,或菜籽油相低于 50%,同时保持 0.5 wt% IBCNFs 的固定悬浮液时,可制备出稳定的 Pickering 乳剂。共焦激光扫描显微镜(CLSM)表明,IBCNFs 有助于在油水界面形成致密的界面层,并在水相中形成三维网络。这有效地阻止了单个油滴之间的聚集和絮凝。此外,流变学研究结果表明,乳液主要具有弹性特性,通过调整 IBCNFs 的辐照剂量可以增强其凝胶强度。此外,环境稳定性实验表明,基于 IBCNFs 的乳液具有极高的耐温性(20-80 °C)和耐 pH 值(4-12)。在高酸(pH = 2)和高离子水平(≥ 40 mM NaCl)条件下会发生絮凝,而不会破坏乳液液滴结构。这项研究为制备可用作乳液稳定剂的高纵横比纤维素纳米纤维提供了一种创新方法。研究还表明,基于 IBCNFs 的 Pickering 乳液具有出色的稳定性和流变特性。IBCNFs 作为一种天然皮克林乳化剂,在稳定各种食品应用、化妆品和药物配方中的水包油乳剂方面具有广阔的前景。
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Innovative Food Science & Emerging Technologies
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