To improve sorghum yield in the milling process, researchers are still working to determine the appropriate method to eliminate the epidermis part of the grain precisely. This research aimed to examine the feasibility of using fluorescence spectroscopy and imaging as a sensor to monitor the milling process by differentiating between whole and milled grains. Fluorescence properties of sorghum in the seed form and after the milled form were reported. For each evaluation, five measurements of the Emission and Excitation Matrix (EEM) fluorescence were carried out through 5 samples taken from different milling replications. The red variety, Suri4, had a lower yield of 45 %, and it was necessary 10 times for polishing to remove the hard external layers. However, white and pale yellow varieties (KD4 and Bioguma) obtained the same yield, although the polishing times were different. The EEM fluorescence spectra showed distinct differences between the three sorghum varieties, even between KD4 and Bioguma, which had the most similar behavior. Two characteristic EEM peaks were identified for Bioguma (Ex280/Em330 and Ex365/Em450 nm) and KD4 (Ex280/Em330 and Ex280/Em450 nm) varieties. However, in the case of Suri4 variety, only one was reported (Ex365/Em450 nm). In this sense, the results demonstrate that both fluorescence spectroscopy and imaging are promising tools for differentiating between sorghum varieties and monitoring the milling process. In particular, image features extracted from fluorescence images under 420 nm excitation provided the clearest separation between varieties and their milling status.
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