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Parasitological And Immunopathological Responses Balb/C Mice With Concomitant Schistosoma Mansoni And Plasmodium Berghei Infections 曼氏血吸虫和伯氏疟原虫合并感染Balb/C小鼠的寄生虫学和免疫病理反应
Pub Date : 2008-12-31 DOI: 10.5580/1f37
M. Kanyugo, H. Ozwara, W. T. Mutahi, D. Yole
Schistosomiasis and malaria often occur in the same area. This study investigated the effect in BALB/c mice harbouring a schistosomiasis-malaria co-infection. Mice infected with S. mansoni were divided into three groups, which were super-infected at weeks 4, 5, and 6 post-infection with P. berghei. There were uninfected and single-infected controls. Sampling was at day 4 and 7 post-super-infection. The following assays were carried out: parasitaemia, perfusion for worm recovery, IgG ELISA, gross and histopathology. Co-infected mice had: higher malaria parasitemia, higher IgG responses, lower worm counts, smaller granulomas compared to the single-infected mice. This study showed that co-infections with schistosome and malaria parasites aggravated malaria severity, as shown by increased parasitemia and severe gross pathology of liver and spleen in the coinfected mice. In contrast, malaria conferred protection to S. mansoni infection, as demonstrated by reduced worm counts and granuloma sizes and high schistosme-specific IgG levels in the co-infected mice.
血吸虫病和疟疾常发生在同一地区。本研究考察了在携带血吸虫病-疟疾合并感染的BALB/c小鼠中的作用。将感染曼氏梭菌的小鼠分为三组,分别在感染柏氏梭菌后第4、5、6周进行超感染。对照组分为未感染组和单一感染组。在超感染后第4天和第7天取样。进行了寄生虫血症、灌流虫虫恢复、IgG ELISA、大体和组织病理学检测。与单一感染小鼠相比,联合感染小鼠有:较高的疟疾寄生虫血症,较高的IgG反应,较低的蠕虫计数,较小的肉芽肿。本研究表明,血吸虫和疟疾寄生虫的共感染加重了疟疾的严重程度,表现为共感染小鼠的寄生虫血症增加,肝脏和脾脏的大体病理严重。相比之下,疟疾对曼氏梭菌感染具有保护作用,这可以通过在共感染小鼠中减少蠕虫数量和肉芽肿大小以及高血吸虫特异性IgG水平来证明。
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引用次数: 10
Deforestation and Malaria in sub-Saharan Africa: an overview 撒哈拉以南非洲的森林砍伐和疟疾:概述
Pub Date : 2008-12-31 DOI: 10.5580/2204
C. Uneke
Malaria remains the most complex and overwhelming health problem in sub-Saharan Africa. The disease is compounded by the generally poor social, environmental and economic conditions in the region. Most sub-Saharan African countries are experiencing unprecedented rate of population growth which has led to uncontrolled and unsustainable exploitation of natural resources, especially the forests resources. Through the process of forest clearing, deforestation alters every element of local ecosystems such as microclimate, soil, and aquatic conditions, and most significantly, the ecology of local flora and fauna, including malaria vectors. Mosquitoes are highly sensitive to environmental changes because of deforestation: their survival, density, and distribution are dramatically influenced by small changes in environmental conditions, such as temperature, humidity, and the availability of suitable breeding sites. Changes in mosquito ecology and human behaviour patterns in deforested regions influence the transmission of malaria and deforestation has therefore been shown to increase the risk of malaria transmission in sub-Saharan. Because deforestation is a process that cannot be readily controlled for a variety of political and economic reasons, investigations and assessments of possible impacts of future deforestation will be crucial to minimize the ecological degradation caused by human activities and to control epidemics of malaria.
疟疾仍然是撒哈拉以南非洲最复杂和最严重的健康问题。该地区普遍较差的社会、环境和经济条件使该疾病更加严重。大多数撒哈拉以南非洲国家正在经历前所未有的人口增长速度,这导致了对自然资源,特别是森林资源的不受控制和不可持续的开发。通过砍伐森林的过程,森林砍伐改变了当地生态系统的每一个要素,如小气候、土壤和水生条件,最重要的是,改变了当地动植物的生态,包括疟疾病媒。由于森林砍伐,蚊子对环境变化高度敏感:它们的生存、密度和分布受到环境条件的微小变化的极大影响,例如温度、湿度和适宜繁殖地点的可用性。森林砍伐地区蚊子生态和人类行为模式的变化影响疟疾的传播,因此,森林砍伐已被证明增加了撒哈拉以南地区疟疾传播的风险。由于森林砍伐是一个由于各种政治和经济原因无法轻易控制的过程,因此,对未来森林砍伐可能产生的影响进行调查和评估对于尽量减少人类活动造成的生态退化和控制疟疾流行病至关重要。
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引用次数: 8
Field Trial Tests On The Efficacy And Residual Effects Of Bistar® 10wp On Mosquitoes And Other Household Arthropod Pests Bistar®10wp对蚊虫和其他家庭节肢动物害虫的药效和残留效果的田间试验
Pub Date : 2008-12-31 DOI: 10.5580/9e7
N. Ozumba, A. Onyido, E. Nwosu, C. Ekwunife, E. S. Amadi
A field test on the efficacy and residual effect of Bistar® 10WP on mosquitoes and other household arthropod pests was conducted at Agu-Owa and Ugbo-Oghe communities in Enugu East Local Government Area of Enugu State, Nigeria. Eighteen four-bedroom apartments noncontiguous with each other and infested with bedbugs and other homestead arthropod pests were selected for the studies from the two communities. In each area, Bistar® 10WP was sprayed in the inside wall surfaces of 3 four-bedroom apartments, lamba cyhalothrin (ICON), was sprayed in another set of 3 four-bedroom apartments and the last 3 houses were used as controls. Pre-and post insecticide application vector monitoring were carried out in the two areas to determine the homestead vector abundance. Insecticide bioassay was carried out to determine the residuality of Bistar® 10WP. Pre-insecticide application vector studies revealed 532 homestead arthropod pests with cockroaches 293(55.08%) and bedbugs 201(37.78%) constituting the bulk of the collections. Post-spray vector evaluation revealed no homestead arthropod in the sprayed areas except in the controls. Bioassay studies showed that Bistar® 10WP compared well with ICON in its efficacy and residuality. Bistar® 10WP was highly accepted by the people as it was odourless and non-irritating to the body and sense organs. More studies were recommended on its efficacy in controlling aquatic and field pests like Glossina species.
在尼日利亚埃努古州埃努古东部地方政府区的aguu - owa和Ugbo-Oghe社区进行了Bistar®10WP对蚊虫和其他家庭节肢动物害虫的药效和残留效果的现场试验。研究人员从两个社区中选择了18套四居室公寓作为研究对象,这些公寓互不相连,而且臭虫和其他家庭节肢动物害虫猖獗。在每个区域,将Bistar®10WP喷洒在3个四卧室公寓的内墙表面,在另一组3个四卧室公寓喷洒氯氟氰菊酯(ICON),最后3个房子作为对照。在两个地区进行了施药前后病媒生物监测,确定了两个地区的病媒生物丰度。采用杀虫剂生物测定法测定Bistar®10WP的残留。蚊媒调查结果显示,共捕获家蝇节肢动物532只,其中蜚蠊293只(55.08%),臭虫201只(37.78%)。喷施后媒介生物评价结果显示,除对照区外,喷施区未发现宅基地节肢动物。生物测定研究表明,Bistar®10WP在功效和残留方面优于ICON。Bistar®10WP因其无气味,对身体和感觉器官无刺激而受到人们的高度认可。建议进一步研究其对水田害虫如绿蝇的防治效果。
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引用次数: 3
Prevalence of sickle cell disease in tribal adolescents of the South Gujarat region, India 印度南古吉拉特邦地区部落青少年镰状细胞病患病率
Pub Date : 2008-12-31 DOI: 10.5580/56f
Bipin Vasava, Rajesh K. Chudasama, Naresh R Godara, R. Srivastava
Objectives: to create awareness, to screen samples of school adolescents and then to reach their community through them by doing surveillance for sickle cell diseaseSettings & Design: School adolescents studying in St Xavier`s high school and Vanraj high school of Umarpada taluka of Surat district were included in the field based cross-sectional studyMethod: After taking permission from school authority, pre-test (n=881) and post-test (n=743) evaluation was done to asses their knowledge regarding SCD. Blood samples of 948 school adolescents were taken for DTT test and then for electrophoresis. Blood samples of motivated parents and friends of those adolescents found positive for DTT was taken in subsequent visit and results were communicated to them.Results: Out of 1081 adolescents, various aspects of sickle cell disease were assessed among 881 during pre-test and among 743 during post-test evaluation. Blood samples of 948 school adolescents were tested for DTT test. Prevalence of sickle cell disease was found 25.5%. Electrophoresis was done in 242 adolescents who found positive for DTT test, of which 92% found having sickle cell trait and 8% having sickle cell disease. Then electrophoresis done among 64 parents and friends and 24 (37.5%) found positive of which 14 (58%) having sickle cell trait and 10 (42%) having sickle cell disease.
目的:提高认识,筛选学校青少年样本,然后通过监测镰状细胞病,向他们的社区提供信息。环境与设计:在苏拉特区Umarpada taluka的St Xavier高中和Vanraj高中学习的学校青少年被纳入基于实地的横断面研究。在获得学校当局的许可后,进行了测试前(n=881)和测试后(n=743)评估,以评估他们对SCD的了解。对948名在校青少年进行DTT检测和电泳。这些青少年的父母和朋友在随后的访问中采集了DTT阳性的血液样本,并将结果告知他们。结果:在1081名青少年中,881名在测试前评估,743名在测试后评估中评估了镰状细胞病的各个方面。对948名在校青少年进行了DTT检测。镰状细胞病患病率25.5%。对242例DTT检测阳性的青少年进行了电泳,其中92%的青少年有镰状细胞特征,8%的青少年有镰状细胞病。对64名家长和朋友进行电泳,结果显示阳性24人(37.5%),其中镰状细胞性状14人(58%),镰状细胞病10人(42%)。
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引用次数: 7
‘Waiting Room Syndrome’ Observations from a sub district hospital in Kashmir 来自克什米尔一家分区医院的“等候室综合症”观察
Pub Date : 2008-12-31 DOI: 10.5580/1bca
Shabir A Dhar, M. F. Butt, Mohammed Ibrahim Makayee
The Sub district hospital in the area of Kangan in the state of Jammu and Kashmir treats a lot of orthopaedic morbidity related to the low back pain stemming from stemming from the degenerative spine disorders. This hospital has only one orthopaedic specialist to cater to around a daily outdoor of 100-125 patients. Patients have to wait in a queue for up to 6 hours to get an orthopaedic consultation.
查谟和克什米尔邦Kangan地区的分区医院治疗了许多与脊柱退行性疾病引起的腰痛有关的骨科疾病。这家医院只有一名骨科专家,每天要接待大约100-125名室外病人。病人要排6个小时的队才能得到骨科会诊。
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引用次数: 0
Urine colour as a rapid assessment indicator in evaluating the prevalence of Schistosoma haematobium infection in two endemic areas of Benue State-Nigeria. 尿色作为评估尼日利亚贝努埃州两个流行区血血吸虫感染流行率的快速评估指标。
Pub Date : 2008-12-31 DOI: 10.5580/1ccf
R. Houmsou, S. Kela, M. Suleiman, J. Ogidi
Schistosomiasis is a formidable public health problem, especially in sub-Saharan Africa where the majority of cases reside. In the context of having interest upon chemotherapy control, rapid, cheap and fast diagnostic tools and assay play an important role in assessing where treatment methods should be concentrated. We examined whether urine colour observation was correlated with intensity of infection in urinary schistosomiasis as measured by the gold-standard parasitological diagnosis. Using this tool and other proven field diagnostic (reagent strips), we examined 750 urine samples collected from school children and communities in two endemic areas of Benue State-Nigeria. Our findings demonstrate that urine colour observation was significantly associated with infection intensity (r = 0.72, p<.01). Given that parasitological examination is laborious, we showed that urine colour observation was significantly correlated with the indirect diagnosis method, Proteinuria (r = 0.75, p<.01) and Haematuria (0.52, p<.01) widely used at the present time. We suggest that urine colour observation may be useful for diagnostic purposes, and for monitoring and evaluating treatment programs over time. Furthermore, we recommend that additional research should be done to further elucidate the relationship between this technique and other diagnostic methods.
血吸虫病是一个严重的公共卫生问题,特别是在大多数病例居住的撒哈拉以南非洲。在对化疗控制感兴趣的背景下,快速、廉价和快速的诊断工具和检测方法在评估治疗方法应该集中在哪里起着重要作用。我们研究了尿色观察是否与尿血吸虫病感染强度相关,这是通过金标准寄生虫学诊断来衡量的。使用该工具和其他经过验证的现场诊断(试剂条),我们检查了从尼日利亚贝努埃州两个流行地区的学龄儿童和社区收集的750份尿液样本。我们的研究结果表明,尿色观察与感染强度显著相关(r = 0.72, p< 0.01)。鉴于寄生虫学检查费力,我们发现尿色观察与目前广泛使用的间接诊断方法、蛋白尿(r = 0.75, p< 0.01)和血尿(0.52,p< 0.01)有显著相关性。我们认为尿液颜色观察可能有助于诊断目的,并随着时间的推移监测和评估治疗方案。此外,我们建议进行进一步的研究,以进一步阐明该技术与其他诊断方法之间的关系。
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引用次数: 6
Umbilical Cord Care Practices In Konduga Local Government Area Of Borno State North - Eastern Nigeria 尼日利亚东北部博尔诺州孔杜加地方政府区的脐带护理实践
Pub Date : 2008-12-31 DOI: 10.5580/2287
J. Ambe, M. Bello, S. Yahaya, B. Omotara
Good cord care practices reduce the incidence of neonatal morbidity and mortality from neonatal infections and tetanus. While cord care practices vary from place to place, some of can be harmful to the new born. The objective of this study was to identify the practices of cord care in Konduga Local Government Area of Borno State, North-Eastern Nigeria. With the help of medical students during their rural posting, a structured questionnaire was administered to 400 mothers with particular reference to their last delivery.The traditional practices of cord care in this area include application of hot fermentation (31.5%), use of rag and lantern (19.5%), use of Vaseline (9.5%), ash / charcoal (9.3%), groundnut / palm, mangrove oil (8.3%) use of powder (6.5%) and red sand (3.5%). These practices are often harmful, because these substances are often contaminated with bacteria and spores, thus increasing the risk of infection. Very few mothers/care givers use saline water (2.0%), use spirit or disinfectant (8.5%) while in a few the cord is left alone to dry on its own (1.5%), which is a more hygienic way of cord care. Mothers need to know about these harmful practices, what they may cause and the problems associated with the use of these substances. The health care provider should be able to educate the mothers about the harmful practices of cord care. This will go a long way in reducing the morbidity and mortality in the newborns.
良好的脐带护理做法可降低新生儿感染和破伤风的发病率和死亡率。虽然脐带护理做法因地而异,但有些做法可能对新生儿有害。本研究的目的是确定尼日利亚东北部博尔诺州孔杜加地方政府地区脐带护理的做法。在医学生在农村工作期间的帮助下,对400名母亲进行了结构化问卷调查,特别提到了她们最近一次分娩的情况。该地区脐带护理的传统做法包括使用热发酵(31.5%)、使用破布和灯笼(19.5%)、使用凡士林(9.5%)、灰/木炭(9.3%)、花生/棕榈、红树林油(8.3%)、使用粉末(6.5%)和红砂(3.5%)。这些做法往往是有害的,因为这些物质往往被细菌和孢子污染,从而增加了感染的风险。很少有母亲/护理人员使用盐水(2.0%),使用酒精或消毒剂(8.5%),而在少数情况下,脐带被单独晾干(1.5%),这是一种更卫生的脐带护理方式。母亲们需要了解这些有害做法、它们可能造成的后果以及与使用这些物质有关的问题。卫生保健提供者应该能够教育母亲关于脐带护理的有害做法。这将大大有助于降低新生儿的发病率和死亡率。
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引用次数: 32
Cryptosporidium and other Intestinal Protozoans in Children with Diarrhoea in Lagos, Nigeria 尼日利亚拉各斯儿童腹泻中的隐孢子虫和其他肠道原生动物
Pub Date : 2008-12-31 DOI: 10.5580/4c4
O. Wellington, Okangba Chika, Ojuromi Teslim, Oladipo Olarinre Oladosu, Fa Adetayo, I. Godswill
As reports of protozoan intestinal parasites in a mega city such as Lagos, Nigeria are scanty and not updated, this study reports the prevalence of intestinal parasites, especially Cryptosporidium species in One hundred and five (105) children less than five years that presented with gastroenteritis in a major tertiary hospital. Standard parasitologic techniques for stool examination and special stains showed cysts and oocysts of protozoan parasites. The overall prevalence rate of intestinal parasites was 27.6%. E.histolytica/dispar and Cryptosporidium oocyst were the most prevalent (9.5% and 17.1% respectively). The age with the highest infectivity was the 4 -5 year-old children from whom the following parasites were detected: Cryptosporidium oocysts (27.6%), Giardia lamblia (4.8%) and E. histolytica/dispar (9.5%). The infectivity of protozoan intestinal parasite was significantly higher in the 4 -5 age-groups (P< 0.001). The findings of this study (especially the detection of Cryptosporidium oocysts) underscores the need to broaden the laboratory work-up in children that presents with gastroenteritis to include special staining technique so as to properly manage the condition. No intestinal helminth was detected among these children.
由于尼日利亚拉各斯等大城市原生动物肠道寄生虫的报道很少,而且没有更新,本研究报告了在一家主要三级医院就诊的105名5岁以下肠胃炎儿童中肠道寄生虫的流行情况,特别是隐孢子虫。粪便检查的标准寄生虫学技术和特殊染色显示原生动物寄生虫的囊肿和卵囊。肠道寄生虫总患病率为27.6%。溶组织芽胞杆菌和隐孢子虫卵囊感染率最高(分别为9.5%和17.1%)。传染性最高的年龄为4 ~ 5岁儿童,检出的寄生虫分别为隐孢子虫卵囊(27.6%)、贾第鞭毛虫(4.8%)和溶组织芽胞杆菌(9.5%)。4 ~ 5岁年龄组肠道原虫传染性显著增高(P< 0.001)。这项研究的发现(特别是隐孢子虫卵囊的检测)强调了扩大胃肠炎患儿的实验室检查的必要性,包括特殊的染色技术,以便适当地控制病情。未检出肠道蠕虫。
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引用次数: 11
Helicobacter pylori Infection in Patients with Functional Dyspepsia in Jamaica 牙买加功能性消化不良患者幽门螺杆菌感染
Pub Date : 2008-12-31 DOI: 10.5580/3ad
Michael G. Lee, H. Emery, D. Whittle, D. Jackson, E. Donaldson
Functional dyspepsia (FD) is a common clinical problem associated with substantial health care cost. H. pylori infection may be an etiological factor in some patients. This study determined the prevalence of H. pylori infection in Jamaican patients with FD. Thirty patients with FD seen between 2005–2007 were studied. All patients had dyspepsia and normal endoscopy of the upper gastrointestinal tract. The prevalence of H. pylori infection was 33%. The mean age was 47 years. 70% were female and resided mainly in urban areas. The most frequent presenting symptom was epigastric pain. All patients had access to piped water and refrigeration. Forty percent of infected patients had a history of alcohol consumption compared to 15% in non-infected patients. In H. pylori positive patients, 20% were cigarette smokers, compared to 5% in negative patients.
功能性消化不良(FD)是一种常见的临床问题,与大量的医疗保健费用相关。幽门螺杆菌感染可能是某些患者的病因。本研究确定了牙买加FD患者幽门螺杆菌感染的患病率。研究了2005-2007年间30例FD患者。所有患者均有消化不良,上消化道内镜检查正常。幽门螺杆菌感染率为33%。平均年龄为47岁。70%为女性,主要居住在城市地区。最常见的症状是胃脘痛。所有病人都有自来水和冰箱。40%的感染患者有饮酒史,而非感染患者的这一比例为15%。在幽门螺杆菌阳性的患者中,20%是吸烟者,而在阴性患者中,这一比例为5%。
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引用次数: 7
Potential Human Pathogens (Bacteria) and their Antibiogram in Ready–to–eat Salads sold in Calabar, South-South, Nigeria 尼日利亚南南卡拉巴尔市销售的即食沙拉中潜在的人类病原体(细菌)及其抗生素
Pub Date : 2008-12-31 DOI: 10.5580/763
S. Udo, I. Andy, A. Umo, M. Ekpo
A total of 150 readyto-eat salad samples were collected from various sources in Calabar and analyzed bacteriologically using standard cultural methods. The samples included 50 from fast food centers, 50 from wedding reception parties and 50 from birthday reception parties all within Calabar metropolis between October 2006 and January 2007. All the bacterial isolates were characterized using the API system and subjected to antimicrobial susceptibility test using the Agar diffusion method. The probable human pathogens isolated were Staphylococcus aureus 35 (41.18%), Escherichia coli 21(24.71%), Klebsiella aerogenes 11(12.94%), Salmonella Enterica var Typhimurium 3(3.53%)., and Bacillus species15(17.65%). Rates of contamination in relation to the various sources were: samples from fast food centers 40.0%, birthday party samples 52.0% and 78.0% for samples from wedding reception parties. Antimicrobial susceptibilities ranged from 63.64% to 84.85%. These findings show that ready to eat salads are commonly contaminated with potential human pathogens that carry multiple atntimicrobial resistant genes. High standards of personal hygiene, pre-processing disinfection and proper storage temperatures should always be observed during preparation and distribution of fresh vegetable and fruit salads.
从卡拉巴尔的不同来源收集了150份即食沙拉样品,并使用标准培养方法进行了细菌学分析。这些样本包括2006年10月至2007年1月期间在卡拉巴尔市的快餐店、婚宴和生日宴会各50个。采用API体系对分离菌株进行鉴定,并用琼脂扩散法进行药敏试验。检出的可能致病菌为金黄色葡萄球菌35株(41.18%)、大肠杆菌21株(24.71%)、产气克雷伯菌11株(12.94%)、伤寒沙门菌3株(3.53%)。芽孢杆菌15种(17.65%)。不同来源的污染率分别为:快餐中心的污染率为40.0%,生日聚会的污染率为52.0%,婚宴的污染率为78.0%。抗菌药物敏感性为63.64% ~ 84.85%。这些发现表明,即食沙拉通常被携带多种抗微生物抗性基因的潜在人类病原体污染。在制备和分发新鲜蔬菜和水果沙拉时,应始终遵守高标准的个人卫生、加工前消毒和适当的储存温度。
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引用次数: 22
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The Internet Journal of Tropical Medicine
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