Pub Date : 2020-01-01DOI: 10.35885/1684-7318-2019-3-396-401
G. Shlyakhtin, V. Tabachishin, M. V. Yermokhin
The feeding of Lacerta agilis was studied by means of the content analysis of lizards' stomachs conducted in the vicinity of Saratov City from April till September 1983, 2003 and 2007. The maximum daily diet was characteristic in July and reached 2.5–2.7 g/day, while the minimum one was in April and September (1.1–1.2 g/day). The highest and lowest numbers of food objects were recorded in May and September, respectively. Insects are the basic food objects of L. agilis, their occurrence frequency being 100% during the whole active-life period. Coleoptera representatives predominated in the diet (25.4–40.5% by number, 43.5–70.9% by weight). The fractions of Lepidoptera, Hymenoptera, Diptera, etc. were somewhat lower. Well-flying and quickly-moving insects predominated in the food spectrum of L. agilis. Rather many invertebrates with cryptic colour, poisonous, stinging and badly smelt ones were frequently met among the nutrition objects. Moreover, vegetable remains (seeds, leaves, flower fragments etc.) and various small stones to act probably as gastrolytes were found in many stomachs.
{"title":"Seasonal Diet Variations of the Sand Lizard (Lacerta agilis) (Lacertidae, Reptilia) in the Northern Lower-Volga Region","authors":"G. Shlyakhtin, V. Tabachishin, M. V. Yermokhin","doi":"10.35885/1684-7318-2019-3-396-401","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-3-396-401","url":null,"abstract":"The feeding of Lacerta agilis was studied by means of the content analysis of lizards' stomachs conducted in the vicinity of Saratov City from April till September 1983, 2003 and 2007. The maximum daily diet was characteristic in July and reached 2.5–2.7 g/day, while the minimum one was in April and September (1.1–1.2 g/day). The highest and lowest numbers of food objects were recorded in May and September, respectively. Insects are the basic food objects of L. agilis, their occurrence frequency being 100% during the whole active-life period. Coleoptera representatives predominated in the diet (25.4–40.5% by number, 43.5–70.9% by weight). The fractions of Lepidoptera, Hymenoptera, Diptera, etc. were somewhat lower. Well-flying and quickly-moving insects predominated in the food spectrum of L. agilis. Rather many invertebrates with cryptic colour, poisonous, stinging and badly smelt ones were frequently met among the nutrition objects. Moreover, vegetable remains (seeds, leaves, flower fragments etc.) and various small stones to act probably as gastrolytes were found in many stomachs.","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42969530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.35885/1684-7318-2019-3-322-334
N. V. Lapshin, M. Matantseva, S. Simonov
{"title":"Nest Site Selection and Nest Construction in the Willow Warbler (Phylloscopus trochilus L., 1758) (Sylviidae, Aves) in the Tajga Zone of Northwest Russia","authors":"N. V. Lapshin, M. Matantseva, S. Simonov","doi":"10.35885/1684-7318-2019-3-322-334","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-3-322-334","url":null,"abstract":"","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41983099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.35885/1684-7318-2019-2-174-188
D. B. Gelashvili, I. Solovyova, I. Belova, A. G. Tochilina, B. Yakimov, N. Zaznobina, E. D. Molkova, N. A. Filashikhin
indices (Shannon’s species diversity index, Simpson’s index of dominance, Margalef’s species richness index, and Pielou’s species evenness index) showed that an increase in the specific diversity of microbial communities is accompanied by a regular decrease in dominance and an increase in evenness. Analysis of the group of dominant species identified in the buses of the three route groups revealed that the species similarity of the dominant species, estimated by the value of Koch’s index, was only 0.14, which could be explained by the presence of only one common species ( Acinetobacter lwoffii) . Studies of the microbial community of urban transport present a perspective for solving the sanitary, hygienic and environmental aspects of the integrated problem of ensuring the ecological safety of the urban environment.
{"title":"Ecological Structure of Public Transport Microbiocoenosis","authors":"D. B. Gelashvili, I. Solovyova, I. Belova, A. G. Tochilina, B. Yakimov, N. Zaznobina, E. D. Molkova, N. A. Filashikhin","doi":"10.35885/1684-7318-2019-2-174-188","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-2-174-188","url":null,"abstract":"indices (Shannon’s species diversity index, Simpson’s index of dominance, Margalef’s species richness index, and Pielou’s species evenness index) showed that an increase in the specific diversity of microbial communities is accompanied by a regular decrease in dominance and an increase in evenness. Analysis of the group of dominant species identified in the buses of the three route groups revealed that the species similarity of the dominant species, estimated by the value of Koch’s index, was only 0.14, which could be explained by the presence of only one common species ( Acinetobacter lwoffii) . Studies of the microbial community of urban transport present a perspective for solving the sanitary, hygienic and environmental aspects of the integrated problem of ensuring the ecological safety of the urban environment.","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45720322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.35885/1684-7318-2019-2-159-173
O. A. Bukhareva, A. Bykov
Animal a The paper considers the main natural changes associated with climatic fluctuations and economic activity in a clayey semidesert of the Volga-Ural interfluve over the past 250 years. The authors distinguish four climatic periods determined by the humideness of the territory, namely: the mid-18 th century – 1820s; 1830s – 1950s; 1960s – the end-1980s; and 1990s – 2010s. For each period, features of the area are described, related to humidification and human economic activity, and, as a result, changes in flora and fauna characteristics of the region. In the clayey semi-desert in the Trans-Volga region, only the existence and character of aquatic and near-water communities is shown to directly de-pend on climatogenic factors. Most changes in the animal world of other community types reflect various stages and forms of the economic use of the territory and are only corrected by climatic changes. The ravine forests in lake depressions existed until the 19 th century ensured the presence of a complex of ravine-forest species in the regional fauna. After the deforestation of these communities, some part of the species of this complex completely disappeared from the region, some part became synanthropes, and the other one firstly disappeared but later returned to the region, after the appearance of polydominant tree-bush communities in lake depressions and artificial forest belts and gardens on the plain. Because of the strong grazing during an arid climatic period, there was a change of steppe plant associations on the plain to desert ones, so that some steppe animal species also disappeared from the region with some desert ones having come to their place. The plowing of plain areas has led to the appearance of species in the region, which use plowing as their feeding sites. Various forms of human development of the territory have led to the arrival of a number of species from other regions. Acknowledgments : This work
{"title":"Main Trends in the Vertebrate Animal Population Dynamics in a Clayey Semidesert of the Trans-Volga Region Since the Mid-18th Century","authors":"O. A. Bukhareva, A. Bykov","doi":"10.35885/1684-7318-2019-2-159-173","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-2-159-173","url":null,"abstract":"Animal a The paper considers the main natural changes associated with climatic fluctuations and economic activity in a clayey semidesert of the Volga-Ural interfluve over the past 250 years. The authors distinguish four climatic periods determined by the humideness of the territory, namely: the mid-18 th century – 1820s; 1830s – 1950s; 1960s – the end-1980s; and 1990s – 2010s. For each period, features of the area are described, related to humidification and human economic activity, and, as a result, changes in flora and fauna characteristics of the region. In the clayey semi-desert in the Trans-Volga region, only the existence and character of aquatic and near-water communities is shown to directly de-pend on climatogenic factors. Most changes in the animal world of other community types reflect various stages and forms of the economic use of the territory and are only corrected by climatic changes. The ravine forests in lake depressions existed until the 19 th century ensured the presence of a complex of ravine-forest species in the regional fauna. After the deforestation of these communities, some part of the species of this complex completely disappeared from the region, some part became synanthropes, and the other one firstly disappeared but later returned to the region, after the appearance of polydominant tree-bush communities in lake depressions and artificial forest belts and gardens on the plain. Because of the strong grazing during an arid climatic period, there was a change of steppe plant associations on the plain to desert ones, so that some steppe animal species also disappeared from the region with some desert ones having come to their place. The plowing of plain areas has led to the appearance of species in the region, which use plowing as their feeding sites. Various forms of human development of the territory have led to the arrival of a number of species from other regions. Acknowledgments : This work","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42155849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-25DOI: 10.35885/1684-7318-2019-1-106-113
N. Gordeeva, Elizavetta K. Komarevceva
Origanum vulgare is a gynodietic species, in whose populations hermaphroditic individuals with bisexual flowers and female individuals with pistillate flowers grow to-gether. This is a holarctic forest-steppe species, a herbaceous perennial. The variability of the ratio of female to hermaphrodite individuals in 26 coenopopulations of O. vulgare from several habitats was investigated (in 8 populations by our own research in the Southern Siberia, and in 18 populations according to literature data from Eastern Europe). In all habitats the projective cover of the species was indicated by standard geobotanical methods. The plants studied have a long-root life form, therefore, a partial shoot or a partial shrub was used as a counting unit (an individual). The sexual structure of coenopopulations was studied by counting generative individuals on transects with an area of 10 to 20 m 2 . In each population the total number of individuals to be counted was at least 90. A significant positive correlation between the projective cover of O. vulgare in the phytocenosis and the proportion of female individuals in coenopopulations was found (the correlation coefficient r = 0.86, p = 0.001). ANOVA analysis has revealed no statistically significant difference in the proportion of females in 6 versions of various phytocenoses: F emp = 3.36 ( F crit5,5 = 5.1, p = 0.05). Considering features of the biomorphology and reproductive biology of various sexual individuals of O. vulgare , one can suggest that the increase of the projective cover of the species in the phytocenosis and the increase in the female individual proportion in the sexual spectrum depend on the successful growing of the seed regeneration in the phytocenosis.
{"title":"Spectrum Variability of Origanum vulgare L. (Lamiaceae, Magnoliopsida)","authors":"N. Gordeeva, Elizavetta K. Komarevceva","doi":"10.35885/1684-7318-2019-1-106-113","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-1-106-113","url":null,"abstract":"Origanum vulgare is a gynodietic species, in whose populations hermaphroditic individuals with bisexual flowers and female individuals with pistillate flowers grow to-gether. This is a holarctic forest-steppe species, a herbaceous perennial. The variability of the ratio of female to hermaphrodite individuals in 26 coenopopulations of O. vulgare from several habitats was investigated (in 8 populations by our own research in the Southern Siberia, and in 18 populations according to literature data from Eastern Europe). In all habitats the projective cover of the species was indicated by standard geobotanical methods. The plants studied have a long-root life form, therefore, a partial shoot or a partial shrub was used as a counting unit (an individual). The sexual structure of coenopopulations was studied by counting generative individuals on transects with an area of 10 to 20 m 2 . In each population the total number of individuals to be counted was at least 90. A significant positive correlation between the projective cover of O. vulgare in the phytocenosis and the proportion of female individuals in coenopopulations was found (the correlation coefficient r = 0.86, p = 0.001). ANOVA analysis has revealed no statistically significant difference in the proportion of females in 6 versions of various phytocenoses: F emp = 3.36 ( F crit5,5 = 5.1, p = 0.05). Considering features of the biomorphology and reproductive biology of various sexual individuals of O. vulgare , one can suggest that the increase of the projective cover of the species in the phytocenosis and the increase in the female individual proportion in the sexual spectrum depend on the successful growing of the seed regeneration in the phytocenosis.","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42147798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-25DOI: 10.35885/1684-7318-2019-1-47-60
S. Sapelnikov, I. Sapelnikova
The catastrophic decline in the number of the speckled ground squirrel (a previously numerous, now-threatened species) requires the adoption of urgent measurements to protect and restore its local populations. The paper presents the results of our observations of the development of pups in the postnatal period, which is necessary for the development of an integrated approach combining the creation of semi-natural reserve populations of the species in natural mega-enclosures with raising litters in ex situ conditions. Pregnant speckled ground squirrel females (n = 8) mated in nature (in situ) were captured at the Kosyrovsky cemetery (Lipetsk City) in April 2017. The females were observed from their trapping until delivery, pups (n = 32) were observed from their birth to 64–77 days. The survival rate of pups was 59%. The obtained data on the dynamics of body mass and the timing of the appearance of some morphological features allow using the devised scale to estimate the age from birth to one month of age with an accuracy of two–three days. After the age of one month when pups start feeding by their own, their keeping in cages favors fat accumulation, and at the age of 7–8 weeks the weight of the animals from cages and nature becomes similar with the further better advance of the first ones. Further improvements in the methods of keeping pregnant females and their litters ex situ are necessary for more successful growing squirrel pups during the first weeks of their life.
{"title":"Postnatal Development of Speckled Ground Squirrel (Spermo-philus suslicus Guld, 1770) (Sciuridae, Mammalia) in Captivity","authors":"S. Sapelnikov, I. Sapelnikova","doi":"10.35885/1684-7318-2019-1-47-60","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-1-47-60","url":null,"abstract":"The catastrophic decline in the number of the speckled ground squirrel (a previously numerous, now-threatened species) requires the adoption of urgent measurements to protect and restore its local populations. The paper presents the results of our observations of the development of pups in the postnatal period, which is necessary for the development of an integrated approach combining the creation of semi-natural reserve populations of the species in natural mega-enclosures with raising litters in ex situ conditions. Pregnant speckled ground squirrel females (n = 8) mated in nature (in situ) were captured at the Kosyrovsky cemetery (Lipetsk City) in April 2017. The females were observed from their trapping until delivery, pups (n = 32) were observed from their birth to 64–77 days. The survival rate of pups was 59%. The obtained data on the dynamics of body mass and the timing of the appearance of some morphological features allow using the devised scale to estimate the age from birth to one month of age with an accuracy of two–three days. After the age of one month when pups start feeding by their own, their keeping in cages favors fat accumulation, and at the age of 7–8 weeks the weight of the animals from cages and nature becomes similar with the further better advance of the first ones. Further improvements in the methods of keeping pregnant females and their litters ex situ are necessary for more successful growing squirrel pups during the first weeks of their life.","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48095341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-25DOI: 10.35885/1684-7318-2019-1-28-46
A. Mischenko, O. Sukhanova, V. Melnikov, P. N. Amosov
1 Институт проблем экологии и эволюции им. А. Н. Северцова РАН Россия, 119071, Москва, Ленинский просп., 33 2 Русское общество сохранения и изучения птиц Россия, 125009, Москва, Большая Никитская, 6 3 Ивановский государственный университет Россия, 153002, Иваново, просп. Ленина, 136 4 Санкт-Петербургская государственная академия ветеринарной медицины Россия, 196084, Санкт-Петербург, Черниговская, 5 E-mail: almovs@mail.ru
{"title":"Meadow Birds at the Waning Traditional Pasture Animal Husbandry","authors":"A. Mischenko, O. Sukhanova, V. Melnikov, P. N. Amosov","doi":"10.35885/1684-7318-2019-1-28-46","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-1-28-46","url":null,"abstract":"1 Институт проблем экологии и эволюции им. А. Н. Северцова РАН Россия, 119071, Москва, Ленинский просп., 33 2 Русское общество сохранения и изучения птиц Россия, 125009, Москва, Большая Никитская, 6 3 Ивановский государственный университет Россия, 153002, Иваново, просп. Ленина, 136 4 Санкт-Петербургская государственная академия ветеринарной медицины Россия, 196084, Санкт-Петербург, Черниговская, 5 E-mail: almovs@mail.ru","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47863107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-24DOI: 10.35885/1684-7318-2019-1-90-105
E. Khramova, A. Lugovskaya, O. Tarasov
An attempt was made to assess the status of the natural environment in the area of the Eastern Ural radioactive trace (EURT) by bioindication methods. With examples of Potentilla fruticosa plants grown at three sites in the radionuclide 90Sr and 137Cs contamination gradient, in the course of our long-term (2005-2013) survey, it was established that as the irradiation level raised, the leaf size and the leafstalk length decreased, the index of the fluctuating asymmetry of the terminal lobe leaf of increased regardless of the period of vegetation and the year of observation. The morphological characteristics (the length, width and area of the leaf blade and those of the leaf terminal lobe, and the leafstalk length) were measured using digital image analysis, MapInfo software. Statistically significant differences in the morphological characteristics of the leaf were noted only in the most contaminated samples in comparison to the reference one. As the radiation load raised, the inter-annual variability of the leafstalk length increased from low to high level regardless of the vegetation period. When estimating the fluctuating asymmetry of the final leaf fraction, statistically significant differences were found for the plants from the samples exposed to radiation in comparison to the control one. Using the fluctuating asymmetry index, the environment quality was characterized as critical for the EURT impact areas while this index was normal for the background.
{"title":"Assessment of Possible Use of Potentilla fruticosa L. (Rosaceae, Magnoliopsida) for Bioindication of the Natural Environment Status in the Area of the Eastern Ural Radioactive Trace","authors":"E. Khramova, A. Lugovskaya, O. Tarasov","doi":"10.35885/1684-7318-2019-1-90-105","DOIUrl":"https://doi.org/10.35885/1684-7318-2019-1-90-105","url":null,"abstract":"An attempt was made to assess the status of the natural environment in the area of the Eastern Ural radioactive trace (EURT) by bioindication methods. With examples of Potentilla fruticosa plants grown at three sites in the radionuclide 90Sr and 137Cs contamination gradient, in the course of our long-term (2005-2013) survey, it was established that as the irradiation level raised, the leaf size and the leafstalk length decreased, the index of the fluctuating asymmetry of the terminal lobe leaf of increased regardless of the period of vegetation and the year of observation. The morphological characteristics (the length, width and area of the leaf blade and those of the leaf terminal lobe, and the leafstalk length) were measured using digital image analysis, MapInfo software. Statistically significant differences in the morphological characteristics of the leaf were noted only in the most contaminated samples in comparison to the reference one. As the radiation load raised, the inter-annual variability of the leafstalk length increased from low to high level regardless of the vegetation period. When estimating the fluctuating asymmetry of the final leaf fraction, statistically significant differences were found for the plants from the samples exposed to radiation in comparison to the control one. Using the fluctuating asymmetry index, the environment quality was characterized as critical for the EURT impact areas while this index was normal for the background.","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43839777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious agents have been identified as a major cause of specific types of human cancers worldwide. Several microorganisms have been identified as potential aggravators of ocular adnexal neoplasms; however, given the rarity of these neoplasms, large epidemiological studies are difficult to coordinate. This study aimed to conduct an exhaustive search for pathogenic DNA in lymphoproliferative disorders (LPD) of the ocular adnexa in a total of 70 patients who were diagnosed with LPD of the ocular adnexa between 2008 and 2013. Specimens were screened for bacterial, viral, fungal, and parasitic DNA by multiplex polymerase chain reaction (PCR) and quantitative real-time PCR. Among cases of conjunctival mucosa-associated lymphoid tissue lymphoma, human herpes virus (HHV)-6, HHV-7, chlamydia, Epstein-Barr virus (EBV) and bacterial 16S ribosomal DNA were detected. In cases of IgG4-related ocular disease, similar pathogens were detected but in a larger number of patients. Our PCR assays detected DNAs of various infectious agents in tumor specimens, especially HHV6, HHV7, and EBV, with different positive rates in various types of LPD. Chronic inflammatory stimulation or activation of oncogenes from these infectious agents might be involved in the pathogenesis of LPD of the ocular adnexa.
{"title":"Comprehensive polymerase chain reaction assay for detection of pathogenic DNA in lymphoproliferative disorders of the ocular adnexa.","authors":"Yoshihiko Usui, Narsing A Rao, Hiroshi Takase, Kinya Tsubota, Kazuhiko Umazume, Daniel Diaz-Aguilar, Takeshi Kezuka, Manabu Mochizuki, Hiroshi Goto, Sunao Sugita","doi":"10.1038/srep36621","DOIUrl":"10.1038/srep36621","url":null,"abstract":"<p><p>Infectious agents have been identified as a major cause of specific types of human cancers worldwide. Several microorganisms have been identified as potential aggravators of ocular adnexal neoplasms; however, given the rarity of these neoplasms, large epidemiological studies are difficult to coordinate. This study aimed to conduct an exhaustive search for pathogenic DNA in lymphoproliferative disorders (LPD) of the ocular adnexa in a total of 70 patients who were diagnosed with LPD of the ocular adnexa between 2008 and 2013. Specimens were screened for bacterial, viral, fungal, and parasitic DNA by multiplex polymerase chain reaction (PCR) and quantitative real-time PCR. Among cases of conjunctival mucosa-associated lymphoid tissue lymphoma, human herpes virus (HHV)-6, HHV-7, chlamydia, Epstein-Barr virus (EBV) and bacterial 16S ribosomal DNA were detected. In cases of IgG4-related ocular disease, similar pathogens were detected but in a larger number of patients. Our PCR assays detected DNAs of various infectious agents in tumor specimens, especially HHV6, HHV7, and EBV, with different positive rates in various types of LPD. Chronic inflammatory stimulation or activation of oncogenes from these infectious agents might be involved in the pathogenesis of LPD of the ocular adnexa.</p>","PeriodicalId":33231,"journal":{"name":"Povolzhskii ekologicheskii zhurnal","volume":"1 1","pages":"36621"},"PeriodicalIF":3.8,"publicationDate":"2016-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57693789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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