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Biostatistical analysis on incidence of bacteria 细菌发病率的生物统计学分析
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000157
B. Zhao, X. Jiang, J. Cao, K. Huang
This study investigated the incidence of bacteria and their antibiogram profile urine samples collected from students of the Northwest A&F University, Yangling, Shaanxi, China. A total of thirty (30) midstream urine samples were collected and bacteria in the samples were isolated using the spread plate method after which biochemical characterization was carried out. Escherichia coli, Pseudomonas spp , Klebsiella species , Salmonella species and Proteus species were the bacterial species isolated after which Kirby-Baurer disc diffusion antimicrobial assay was used to test their sensitivity against various antibiotics, such as Gentamycin, Cotrimozaxole, Ceftriaxone, Amoxyclay, Levofloxacin and Clavatin. From the study, Proteus spp shows zones of inhibition of (7mm for Cotrimozaxole), (5mm for Ceftriaxone), 5mm (for Gentmycin), (5mm), and 4mm (for Amoxyclav). E. coli 6.2 mm (Gentamycin), 3.6 mm (Levofloxacin), and 3.6 mm (Levofloxacin) zones of inhibition respectively . Gentamycin obtained 4.00mm, Levofloxacin 2.00mm and erythromycin 1.00 mm against Pseudomonas spp . Zones of inhibition were only observed on Clavatin (2.1 mm) for Salmonella species, while Klebsiella species was however , resistance to all the antibiotics tested . The highest sensitivity was observed in Cotrimozaxole which obtained a diameter of 7mm for Proteus spp ., while the lowest zone was recorded for Pseudomonas species against Erythromycin with a diameter of 1 mm. This study revealed that most of the isolates were resistant to the antimicrobial agents the implication of antibacterial resistance as shown in the study. This study therefore recommends that proper hygiene should be observed before ingestion of food and water.
本研究调查了陕西杨凌西北农林科技大学学生尿液中细菌的发生率及其抗生素谱。采集中游尿液样本30份,采用涂布平板法分离样本中的细菌,进行生化表征。分离出大肠杆菌、假单胞菌、克雷伯氏菌、沙门氏菌和变形杆菌,采用Kirby-Baurer圆盘扩散抗菌试验检测其对庆大霉素、复方曲松、头孢曲松、阿莫克莱、左氧氟沙星和克拉瓦汀等多种抗生素的敏感性。从研究中,Proteus spp显示出(Cotrimozaxole 7mm), (Ceftriaxone 5mm), (Gentmycin 5mm), (5mm)和(amoxyclv 4mm)的抑制区。大肠杆菌分别有6.2 mm(庆大霉素)、3.6 mm(左氧氟沙星)和3.6 mm(左氧氟沙星)的抑制区。庆大霉素为4.00mm,左氧氟沙星为2.00mm,红霉素为1.00 mm。沙门氏菌仅在Clavatin (2.1 mm)上有抑制区,而克雷伯菌对所有抗生素均有耐药。Cotrimozaxole对变形杆菌的敏感性最高,直径为7mm,而假单胞菌对红霉素的敏感性最低,直径为1mm。本研究表明,大多数分离株对抗菌药物具有耐药性,研究结果表明其具有耐药性。因此,这项研究建议在摄入食物和水之前应注意适当的卫生。
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引用次数: 0
Comparative studies on squab growth performance and egg morphometrical attributes of different pigeon breeds 不同鸽种雏鸽生长性能及蛋形态计量特性的比较研究
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000189
O. Islam, Shameema Khatun, M. Famous, M. Uddin
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引用次数: 0
Bull and cow-level prevalence of paratuberculosis in Lidia’s cattle breed from the Azores 来自亚速尔群岛的利迪亚牛品种中公牛和奶牛副结核病的流行程度
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000188
J. Pacheco-Lima, Faria N Silva, L. ValadãoH, Moreira da Silva Jf
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引用次数: 0
Analyze of elements of the productive chain of fingerlings of rheophilic species: Pilot study in the Amazon region 亚马逊河流域嗜流物种鱼种生产链要素分析
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000187
N. B. Medeiros, Marcela Cristina Flexa do Amaral, Geciele Santos Cruz, Leandro de Lima Sousa, I. Hamoy, M. Rodrigues
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引用次数: 0
Effect of feeding different levels of protein supplementation on the growth performance of young snails (Archachatina marginata) in the western highlands of Cameroon 饲喂不同水平的蛋白质对喀麦隆西部高地小蜗牛生长性能的影响
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000186
Ngwarh Perpetua Tuncha, A. T. Niba, Ndzi Roger Banla
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引用次数: 0
Correlations between states of amino acids and hematology or plasma biochemistry in calves within 24 hours after birth 犊牛出生后24小时内氨基酸状态与血液学或血浆生化的相关性
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000170
Akashi N, Azuma K, T. T, Kawamoto H, Amaha T, Yamashita M, Osaki T, Ito N, O. Y, Murahata Y, Imagawa T
The present study examined correlations between state of free amino acids and hematological or plasma biochemistry, using blood samples acquired from Japanese- black calves within 24 hours after birth. All of free amino acids demonstrated negative correlations with red blood cell counts, hemoglobins and hematocrits. The most significant positive correlation was recorded between γ -glutamyl transpeptidase and glutamic acid, with a linear regression of y=0.025 x+39.99, where x corresponds to γ -glutamyl transpeptidase and y corresponds to glutamic acid (r=0.88, p<0.001). This suggests glutamic acid concentration may relate to the degree of colostrum intake in Japanese-black newborn calves. transpeptidase; AA: Amino acid; Thr: Threonine; Val: Valine; Met: Methionine; Ile: Isoleucine; Leu: Leucine; Phe: Phenylalanine; Lys: Lysine; His: Histidine; Trp: Tryptophan; Arg: Arginine; EAA: Essential AAs; Asp: Aspartic acid; Ser: Serine; Asn: Asparagine; Glu: Glutamic acid; Gln: Glutamine; Gly: Glycine; Ala: Alanine; Tyr: Tyrosine; Pro: Proline,; Cit: Citrulline; Orn: Ornithine; NEAA: Nonessential AAs; TAA: Total AAs.
本研究利用出生后24小时内采集的日本黑犊牛血液样本,检验了游离氨基酸状态与血液学或血浆生化之间的相关性。所有的游离氨基酸都与红细胞计数、血红蛋白和红细胞比容呈负相关。γ -谷氨酰转肽酶与谷氨酸呈显著正相关,线性回归为y=0.025 x+39.99,其中x对应γ -谷氨酰转肽酶,y对应谷氨酸(r=0.88, p<0.001)。这表明谷氨酸浓度可能与日本黑犊牛初乳摄取量有关。转肽酶;AA:氨基酸;刺:苏氨酸;瓦尔:缬氨酸;遇见:蛋氨酸;Ile:异亮氨酸;低浓缩铀:亮氨酸;法:苯丙氨酸;赖氨酸:赖氨酸;他:组氨酸;Trp:色氨酸;参数:精氨酸;EAA:必要的AAs;Asp:天冬氨酸;爵士:丝氨酸;Asn:天冬酰胺;Glu:谷氨酸;Gln:谷氨酰胺;g:甘氨酸;阿拉巴马州:丙氨酸;酪氨酸,酪氨酸;职业:脯氨酸;Cit:瓜氨酸;内在的:鸟氨酸;NEAA:非必要的AAs;TAA:总aa。
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引用次数: 2
Denaturing gradient gel electrophoresis (DGGE): A tool to assess diversity of Mycoplasma species in small ruminants 变性梯度凝胶电泳(DGGE):一种评估小反刍动物支原体物种多样性的工具
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000163
G. Macaluso, A. Parco, S. Tumino, L. Galuppo, R. Puleio, N. Raj, G. Loria
Mycoplasma diseases of livestock such as contagious bovine pleuropneumonia, contagious caprine pleuropneumonia and contagious agalactia still represent major problems for animal health authorities worldwide. Many significant improvements have been seen as a result of research into these degenerate bacteria, mainly in the area of diagnosis, with the availability of specific, effective new molecular tools to detect small quantities of these pathogens (some of them highly fastidious, unculturable and often in mixed bacterial culture) directly from clinical samples. The objective of the present study was to identify and differentiate Mycoplasma species of sheep, goats and cattle by a new diagnostic test based on PCR of the 16S rRNA gene with specific primers and separation of the PCR product according to primary sequence using denaturing gradient gel electrophoresis (DGGE). Detection and identification results were compared with the ones obtained by sequence analysis. The development of a PCR DGGE offers advantages of a rapid identification of many Mycoplasma species for which no specific PCR is yet available, enabling the differentiation of animal Mycoplasma species. It represents a significant improvement from conventional culture and serological tests in term of specificity and time to perform. *Correspondence to: Nicholas RAJ, The Oaks, Nutshell Lane, Farnham, Surrey, UK, E-mail: robin.nicholas@btinternet.com Received: August 20, 2019; Accepted: August 28, 2019; Published: August 30, 2019 Introduction Mycoplasmas cause a wide range of disease in both humans and animals and are commonly associated with pneumonia, arthritis, conjunctivitis, infertility and abortion. Mycoplasmas are highly fastidious, typically taking weeks to culture while many serological tests are non-specific and poorly sensitive. More recently PCRs have been used to detect Mycoplasma species, but it is not feasible to develop PCR tests for each species. Consequently, there is a significant need for a single, generic test that can both detect and differentiate Mycoplasmas. Denaturing gradient gel electrophoresis (DGGE) can theoretically detect single base mutations in DNA [1-3]. Amplicons of the same length but with different nucleotide compositions are separated in a denaturing gradient gel of polyacrylamide, based on their differential denaturation profile. During denaturation, the two strands of a DNA molecule separate or melt at a specific denaturant concentration, and the DNA sequence stops its migration in the gel. PCR DGGE allows the detection and differentiation of mixed and very small amounts of Mycoplasmas showing higher sensitivity than the conventional tests. Using this method McAuliffe et al. [4,5] enabled the differentiation of 67 Mycoplasma species of human and animal origin representing a significant improvement on current tests. Diagnosis of Mycoplasma infection can be made directly from clinical samples in less than 24 h and can even detect new and unculturable
牲畜的支原体疾病,如传染性牛胸膜肺炎、传染性山羊胸膜肺炎和传染性无乳病,仍然是世界各地动物卫生当局面临的主要问题。对这些退化细菌的研究已经取得了许多重大进展,主要是在诊断领域,有了专门的、有效的新分子工具,可以直接从临床样本中检测少量这些病原体(其中一些非常挑剔,不可培养,通常是混合细菌培养)。本研究的目的是建立一种新的绵羊、山羊和牛支原体的诊断方法,该方法基于16S rRNA基因的特异引物PCR,并利用变性梯度凝胶电泳(DGGE)对PCR产物进行一级序列分离。并将检测鉴定结果与序列分析结果进行了比较。PCR DGGE的发展提供了快速鉴定许多尚未获得特异性PCR的支原体物种的优势,使动物支原体物种的分化成为可能。在特异性和执行时间方面,它代表了传统培养和血清学测试的显着改进。*通讯:Nicholas RAJ, The Oaks,果果巷,法纳姆,萨里,英国,E-mail: robin.nicholas@btinternet.com收稿日期:2019年8月20日;录用日期:2019年8月28日;支原体在人类和动物中引起广泛的疾病,通常与肺炎、关节炎、结膜炎、不孕症和流产有关。支原体非常挑剔,通常需要数周的时间来培养,而许多血清学测试是非特异性和低敏感性的。最近,PCR已用于检测支原体种类,但不可能为每个物种开发PCR检测方法。因此,迫切需要一种既能检测支原体又能区分支原体的单一通用检测方法。变性梯度凝胶电泳(DGGE)理论上可以检测DNA的单碱基突变[1-3]。长度相同但核苷酸组成不同的扩增子根据其不同的变性特征,在聚丙烯酰胺变性梯度凝胶中分离。在变性过程中,DNA分子的两条链在特定的变性剂浓度下分离或融化,DNA序列停止在凝胶中的迁移。PCR DGGE可以检测和区分混合和非常少量的支原体,显示出比传统测试更高的灵敏度。使用这种方法,McAuliffe等人[4,5]能够区分67种来自人类和动物的支原体,这是对当前测试的重大改进。支原体感染的诊断可以在不到24小时的时间内直接从临床样本中进行,甚至可以检测到新的和不可培养的支原体。我们报道DGGE在小反刍动物致病性和非致病性支原体检测中的应用。
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引用次数: 0
Directors of Veterinary Services in the Anglo-Egyptian Sudan: Leonard Danels (Director, 16 December 1922-19 June 1924), 1913-1924 英埃苏丹兽医服务主任:伦纳德·丹尼尔斯(主任,1922年12月16日至1924年6月19日),1913-1924年
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000149
R. T. Wilson
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引用次数: 0
Biostatistical analysis on chronic disease risk 慢性疾病风险的生物统计学分析
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000160
Zhao B, Jiang X, Cao J, H. K
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引用次数: 1
Biological treatment of crop residues as an option for feed improvement in the tropics: A review 作物残茬生物处理作为热带地区饲料改良的一种选择:综述
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000176
B. Asmare
In tropical countries, roughages feeds are the major diets of ruminant animals which are poor in quality. Hence, improving the nutritive value of feeds of such kind is vital for best utilization for ultimate goal of increased animal production. Animal feeds and feeding practices can be changed by biological catalysts such as fungi with the objective to improve nutritive value and to reduce environmental waste. Biological treatment of such crop residues using white rot fungi can break the ligno- cellulose structure, liberating free cellulose and thus enhancing their feeding value. Biologically treated roughages have higher digestibility for most of the nutrients with an increase in crude protein content as compared to untreated material, besides ensuring more fermentable substrates in the rumen. In addition, treatment of low-quality animal feeds with white rot fungi species increases the protein and ash contents with a reduction of its fibrous fraction. Moreover, biological treatment roughages feed increases the feed intake, digestibility and eventually livestock production and reproduction. However, the application of biological treatment of roughages is limited by lack of biological agents such as the typical fungi or its products (enzymes) and knowledge of utilization of such agents. The other setback is reduction of weight of the final substrate after the treatment in which case a dry matter loss of substrate can be as high as 40% in prolonged incubation with the fungus. Moreover, there is lack of know how to use such technology in most of tropical Africa including Ethiopia. This review was then organized to create awareness on utilization of biological treatment as remedy for poor quality of roughages, optimizing mechanisms must be sought.
在热带国家,粗饲料是反刍动物的主要饲料,但质量较差。因此,提高这类饲料的营养价值对于提高动物产量这一最终目标的最佳利用至关重要。可以通过真菌等生物催化剂改变动物饲料和饲养方法,以提高营养价值和减少环境浪费。利用白腐菌对这类作物秸秆进行生物处理,可以破坏木质纤维素结构,释放游离纤维素,从而提高其饲用价值。与未经处理的原料相比,生物处理的粗饲料对大多数营养物质的消化率更高,粗蛋白质含量也有所增加,此外还确保了瘤胃中有更多的可发酵底物。此外,用白腐真菌处理低质量动物饲料可以增加蛋白质和灰分含量,减少纤维成分。此外,生物处理粗饲料提高了采食量和消化率,最终提高了牲畜的生产和繁殖。然而,由于缺乏诸如典型真菌或其产物(酶)等生物制剂以及对这些制剂的利用知识,粗饲料生物处理的应用受到限制。另一个挫折是处理后最终基质重量的减少,在这种情况下,在与真菌长时间孵育的情况下,基质的干物质损失可高达40%。此外,包括埃塞俄比亚在内的大多数热带非洲国家都缺乏如何使用这种技术的知识。本综述旨在提高人们对利用生物处理作为粗饲料质量差的补救措施的认识,必须寻求优化机制。
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引用次数: 3
期刊
Animal Husbandry, Dairy and Veterinary Science
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