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Serosurvey of schmallenberg virus infection in sheep in Abruzzo, Italy: Short report 意大利阿布鲁佐绵羊施马伦贝格病毒感染的血清调查:简短报告
Pub Date : 2019-11-11 DOI: 10.15761/ahdvs.1000166
Minozzi G, Martino C, Ianni A, Palazzo F, D. Mg, Polli M, Di Paolo G, Giammarino A, Martino G
Infection with Schmallenberg virus (SBV) causes congenital musculoskeletal and vertebral malformations as well as neurological failures in fetuses of several ruminant species. In this study 1038 sheep samples from 10 flocks in the provinces of Chieti, Teramo and Pescara in Italy have been tested for antibodies against SBV by ELISA test. The purpose of the study was to ascertain the extent of SBV infections in sheep in Italy. The results of the ELISA test identified at least one positive animal in 9 of the 10 sheep flocks tested, and a mean within-flock prevalence of 8.57%. Furthermore, large variability of positive animals between flocks was observed (0 and 42.5%). These results demonstrate that SBV was endemic in this region and there could be is a risk of novel SBV infections in the following lambing season, raising serious concerns about its so rapid and pervasive spread. *Correspondence to: Minozzi G, Department of Veterinary Medicine, Università degli Studi di Milano, Milan, Italy, Tel: +390250318035; E-mail: giulietta.minozzi@unimi.it
施马伦伯格病毒(SBV)感染会导致几种反刍动物胎儿的先天性肌肉骨骼和脊椎畸形以及神经功能衰竭。本研究采用ELISA法检测了意大利基耶蒂省、特拉莫省和佩斯卡拉省10个羊群的1038只羊的SBV抗体。该研究的目的是确定意大利绵羊中SBV感染的程度。ELISA检测结果显示,在10个检测羊群中,9个羊群中至少有1只阳性动物,平均群内流行率为8.57%。此外,观察到不同畜群之间阳性动物的差异很大(0 ~ 42.5%)。这些结果表明,SBV在该地区是地方性的,并且在接下来的产羔季节可能存在新型SBV感染的风险,引起了对其如此迅速和广泛传播的严重关注。*通讯:Minozzi G,米兰大学兽医学系,米兰,意大利,电话:+390250318035;电子邮件:giulietta.minozzi@unimi.it
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引用次数: 0
Effects of an inactivated vaccine for bovine mycoplasmosis on calves naturally affected with Mycoplasma bovis 牛支原体病灭活疫苗对自然感染牛支原体小牛的影响
Pub Date : 2019-08-13 DOI: 10.15761/ahdvs.1000161
R. Nicholas, G. Loria, S. Catania, R. Piccinini
Three autogenous vaccine trials were carried out on farms where Mycoplasma bovis had been identified as a major pathogen. The first trial was carried on a veal farm in the Lombardia region of northern Italy. Vaccine, prepared by saponising an M bovis strain taken from the farm some months before, was given as single inoculation to 24 calves on arrival while 19 were left unvaccinated. Six months later calves were sent to the abattoir where lungs were examined for gross pathological lesions. The mean weight of the vaccinated group was higher though not significantly so and mean lesion scores were similar; however the percentage of vaccinated calves with severe lung lesions and pleuritis was lower than in the non vaccinates. A second trial was carried out in northern England on a farm where monthly batches of male calves from a nearby dairy herd were reared under a feedlot system. One group of 27 calves were vaccinated with a saponised strain of M bovis isolated from the lungs of a pneumonic calf on the farm prior to the start of the trial. A second batch of 25 calves was left unvaccinated. The groups of calves were monitored for nine months prior to slaughter and records kept of antibiotic usage and mortality. The mortality rates in the vaccinated calves were about 15% compared to 28% in unvaccinated calves; however there was no difference in the number of times calves were treated for respiratory disease. A third batch of calves, treated with oxytetracycline on arrival because a number were already showing respiratory signs, had a lower mortality rate and fewer subsequent treatments. A third trial was carried out on a milk veal unit in the Veneto region of Italy. A group of 19 calves were vaccinated on arrival with a saponised isolate taken earlier from the farm; a similar number of calves of the same batch were left unvaccinated. After 6 months animals were routinely processed at the abattoir and lungs inspected. Results showed that vaccinated calves had higher mean body weights and although similar mean lung scores there was a smaller percentage of vaccinated calves with severe lung lesions and pleuritis. *Correspondence to: Nicholas RAJ, The Oaks, Nutshell Lane, Farnham, Surrey, UK, E-mail: robin.nicholas@btinternet.com
在牛支原体被确定为主要病原体的农场进行了三次自体疫苗试验。第一次试验是在意大利北部伦巴第地区的一个小牛肉农场进行的。疫苗是将几个月前从农场提取的牛分枝杆菌菌株皂化后制成的,在24头小牛抵达时一次性接种,其余19头小牛未接种。六个月后,小牛被送到屠宰场,在那里检查肺部的大体病理病变。接种疫苗组的平均体重较高,但差异不显著,平均病变评分相似;然而,接种疫苗的小牛患严重肺部病变和胸膜炎的比例低于未接种疫苗的小牛。第二次试验是在英格兰北部的一个农场进行的,该农场在饲养场系统下饲养每月一批的来自附近奶牛群的雄性小牛。在试验开始前,一组27头小牛接种了从该农场一只患肺炎的小牛的肺中分离出来的皂化的牛分枝杆菌菌株。第二批25头小牛没有接种疫苗。屠宰前对犊牛组进行了9个月的监测,并保存了抗生素使用和死亡率的记录。接种疫苗的小牛死亡率约为15%,而未接种疫苗的小牛死亡率为28%;然而,小牛接受呼吸道疾病治疗的次数没有差异。第三批小牛在抵达时接受了土霉素治疗,因为一些小牛已经出现呼吸症状,死亡率较低,后续治疗也较少。第三次试验是在意大利威尼托地区的一个小牛肉单位进行的。一组19头小牛在抵达时接种了早些时候从农场提取的皂化分离物;同一批次中,同样数量的小牛没有接种疫苗。6个月后,动物在屠宰场进行常规处理并检查肺部。结果表明,接种疫苗的小牛平均体重较高,尽管平均肺评分相似,但接种疫苗的小牛中有严重肺部病变和胸膜炎的比例较小。*通信:Nicholas RAJ, The Oaks,果壳巷,法纳姆,萨里,英国,E-mail: robin.nicholas@btinternet.com
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引用次数: 0
Directors of veterinary services in the Anglo-Egyptian Sudan: Sampson Charles Jenkin Bennett (Assistant Director and Senior Research Officer 1936-1944), 1925-1944 英埃苏丹兽医服务主任:桑普森·查尔斯·詹金·贝内特(1936-1944年助理主任兼高级研究干事),1925-1944年
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000167
R. T. Wilson
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引用次数: 1
Study on the prevalence of bovine fasciolosis in hirna and its suroundings, western hararghe, Ethiopia 埃塞俄比亚哈拉河西部赫纳及其周边地区牛片形吸虫病流行病学研究
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000158
Girma Y, Delelegn M
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantic, is one the most prevalent helminthes infections of ruminants in different parts of the district. It is one of the most important parasitic diseases that hamper livestock production in Ethiopia. Fasciolosis in cattle is considered to be of a great economic importance by both species of Fasciola. In Ethiopia, there is still a gap for many potential sites of the country and information is not available to review country wide prevalence and economic significance. A cross-sectional study was conducted with the objective of; to determining the prevalence of bovine fasciolosis in Hirna and its surrounding in western Hararghe based on coprological examinations. A total of 384 faecal samples were collected from different sites of the district. The faecal examination shows that out of the examined animals, 75(19.5%) were positive for fasciolosis. Fasciolosis is a common parasitic disease in the study site which are swampy or marshy grazing areas, prevailing favorable condition for the survival of intermediate host, snail. The tenth study site (Awujemijim, Burahisa, Gara-Qufa, Gamachis, KiraKufiss, Libu-Dukeb, Kufa-Kass, Oda-Nagaya, Oda-Belina and Reketa-Fura) prevalence rate as determined from faecal examination indicates a higher Libu-Dukeb (37.83%) followed by Oda-Belina (31.25%) and lower in Kufa-Kass (9.09%) and Burahisa (8.33%). Statistical analysis of the coprological result was made on the basis of sex and age. The influence of age on the prevalence of fasciolosis was found nil (P > 0.05). The result of the study indicated that sex has significant difference (χ2= 8.71, P< 0.05) on the prevalence of fasciolosis. Based on selected site prevalence and local factors, appropriate control strategies pertinent to local situation have been designed and forwarded so as to reduce the disease problem on livestock production. *Correspondence to: Girma Y, Ministry of Agriculture, Addis Ababa, Ethiopia, Tel: 251912906079; E-mail: girmayeshibelay@gmail.com
由肝片形吸虫和巨型片形吸虫引起的片形吸虫病是我区反刍动物最常见的寄生虫感染之一。它是阻碍埃塞俄比亚畜牧生产的最重要的寄生虫病之一。两种片形吸虫都认为牛片形吸虫病具有重要的经济意义。在埃塞俄比亚,该国的许多潜在地点仍然存在差距,而且没有资料来审查全国范围内的流行情况和经济意义。进行了横断面研究,目的是;以粪学检查为基础,确定哈拉河西部希纳及其周边地区牛片形吸虫病的流行情况。在全区不同地点共采集粪便样本384份。粪便检查结果显示,75只(19.5%)呈片吸虫病阳性。片形吸虫病是研究区常见的一种寄生虫病,研究区多为沼泽或沼泽放牧区,对中间寄主蜗牛的生存有利。第10个研究点(Awujemijim、Burahisa、Gara-Qufa、Gamachis、KiraKufiss、Libu-Dukeb、Kufa-Kass、Oda-Nagaya、Oda-Belina和Reketa-Fura)的粪便检查患病率显示,Libu-Dukeb较高(37.83%),其次是Oda-Belina (31.25%), Kufa-Kass(9.09%)和Burahisa较低(8.33%)。按性别、年龄对结果进行统计学分析。年龄对片膜虫病患病率无影响(P > 0.05)。研究结果显示,性别差异有显著性意义(χ2= 8.71, P< 0.05)。根据选定的现场流行情况和当地因素,设计并提出了适合当地情况的控制策略,以减少疾病对畜牧业生产的影响。*通讯:埃塞俄比亚亚的斯亚贝巴农业部,电话:251912906079;电子邮件:girmayeshibelay@gmail.com
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引用次数: 2
Effects of housing on rodent behavior: Wire vs. solid plastic caging 住房对啮齿动物行为的影响:金属丝与固体塑料笼
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000177
Marshall G. Miller, Donald E. Smith, B. Shukitt-Hale
Housing of laboratory animals has changed dramatically in the last 20 years; however, the effect of modern rodent housing on behavior has not been extensively evaluated and published findings are conflicting. In the present study, aged (19 mo) Sprague-Dawley rats were single-housed in either suspended wire-mesh cages or suspended plastic cages for two months. Thereafter, rats completed a battery of balance, coordination, and strength tests, including walking on a horizontal rod, planks of varying widths, and rotarod as well as clinging to an inclined screen and horizontal wire. Rats also completed a working memory task in the water maze. Following introduction to the rodent housing, plastic cage-housed rats initially lost body weight but returned to baseline when water was made available through an additional route. Although no pathologies were observed in the rats’ extremities, analysis of behavioral data showed that plastic-housed rats balanced significantly longer on medium and wide planks and were able to cling longer to an inclined screen. No housing effects were observed in results from the water maze. These findings support concerns that modernization of rodent housing may impact measures of both motor and ingestive behavior.
在过去的20年里,实验动物的居住环境发生了巨大的变化;然而,现代啮齿动物住房对行为的影响尚未得到广泛评估,已发表的研究结果相互矛盾。在本研究中,年龄(19月龄)的Sprague-Dawley大鼠被单独饲养在悬置的金属网笼或悬置的塑料笼中两个月。之后,老鼠完成了一系列的平衡、协调和力量测试,包括在水平杆、不同宽度的木板和旋转杆上行走,以及抓住倾斜的屏风和水平的电线。大鼠还在水迷宫中完成了工作记忆任务。在引入啮齿类动物住房后,塑料笼中的老鼠最初体重下降,但当通过其他途径获得水时,体重恢复到基线。虽然没有观察到老鼠的四肢出现病变,但对行为数据的分析表明,塑料饲养的老鼠在中等和宽的木板上保持平衡的时间明显更长,并且能够更长时间地抓住倾斜的屏幕。在水迷宫的结果中没有观察到住房效应。这些发现支持了啮齿动物住房现代化可能影响运动和摄食行为测量的担忧。
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引用次数: 1
Iodine, an effective substance against the COVID-19 pandemic 碘是对抗COVID-19大流行的有效物质
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000190
Rezaeipuor Mohamad
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引用次数: 0
Study of external parasitic infection in domestic dogs referred to Mazandaran veterinary clinic (Northern Iran) 伊朗北部Mazandaran兽医诊所家犬外部寄生虫感染研究
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000172
N. VahediNouri, A. Salehi, M. Masoumi, A. Mohsen
This study evaluates external parasites in domestic dogs referred to veterinary clinics of Mazandaran Province (Northern Iran). The results of this study show that 110 Dogs (36.6%) infected with external parasites than among 52 Dogs (17.3%) infected with Rhipicephalus bursa . 41 dogs (13.6%) infected with Ctenocephalides canis and 17 dogs (5.6%) infected with both of Rhipicephalus bursa and Ctenocephalides canis . Considering the importance of external parasitic infection in domestic animals and the issue of public health, necessity of hygiene, prevention and control of these factors are very important.
本研究评估了Mazandaran省(伊朗北部)兽医诊所家养狗的外部寄生虫。本研究结果显示,110只犬(36.6%)感染外源寄生虫,52只犬(17.3%)感染囊性鼻头虫。41只犬感染了棘头绦虫(13.6%),17只犬同时感染了囊状棘头绦虫和棘头绦虫(5.6%)。考虑到家畜外源寄生虫感染的重要性和公共卫生问题,对这些因素进行卫生、预防和控制的必要性非常重要。
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引用次数: 0
Effect of humic substances on the mineral composition of chicken meat 腐殖质对鸡肉矿物质组成的影响
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000168
M. Skalická, P. Nad, L. Bujňák, M. Hudák
The study was conducted to determine the concentrations of mineral substances calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn) in the thigh and breast muscle of broiler chicks after application of humic substances. Eighty-one-day-old broiler chicks were randomly divided into 4 groups of 20 animals. Group 1 (G1) was supplemented with 0.7% Humac Natur (HN), G2 with 0.7% Humac Natur Monogastric (HNM) and G3 with 0.5% HNM and control group (CG) received basal diet without any supplements. The samples of muscles were analyzed using flame atomic absorption spectrometry method (AAS). The statistically significantly higher levels of Ca and Zn (p<0.01) were found in breast and thigh muscles in G1 in comparison to the CG. An addition of the 0.7% HNM in G2 caused the significantly higher levels of the Ca (p<0.05) and Zn (p<0.01) in both types of muscles in comparison to the CG. The addition of 0.7% HNM (p<0.05) and 0.5% HNM (p<0.01) in feed significantly reduced the amount of the Cu in the breast muscle versus the addition of 0.7% HN. Significantly lower Zn contents (p<0.01) were found in the breast and thigh muscle of broilers of CG in comparison to the G1 with addition of 0.7% HN. In the group G1 was achieved a strong positive correlation in the thigh muscle between the Mg and the Zn (r=0.9660) after application of 0.7% HN. According to the results, significant positive correlation between the Zn and the Mg (r=0.8187) in the breast muscle was observed in the group G2. In conclusion, HN and HMN can be considered a good additive with a positive impact on the nutrition value of poultry meat.
本试验旨在测定施用腐殖质后肉仔鸡大腿和胸肌中钙、镁、铜、锌等矿物质的含量。选取81日龄肉鸡,随机分为4组,每组20只。1组(G1)在基础饲粮中添加0.7% Humac Natur (HN), G2组添加0.7% Humac Natur单胃(HNM), G3组添加0.5% HNM,对照组(CG)在基础饲粮中不添加任何添加物。采用火焰原子吸收光谱法(AAS)对肌肉样品进行分析。G1期乳腺和大腿肌肉Ca、Zn水平明显高于CG (p<0.01)。与CG组相比,在G2组中添加0.7% HNM使两种肌肉中Ca (p<0.05)和Zn (p<0.01)水平显著升高。饲料中添加0.7% HNM (p<0.05)和0.5% HNM (p<0.01)较添加0.7% HN显著降低了胸肌中Cu的含量。CG组肉鸡胸肌和大腿肌锌含量显著低于添加0.7% HN的G1组(p<0.01)。在G1组中,施0.7% HN后大腿肌Mg与Zn呈极显著正相关(r=0.9660)。结果显示,G2组胸肌中Zn与Mg呈显著正相关(r=0.8187)。综上所述,HN和HMN可作为一种良好的添加剂,对禽肉的营养价值有积极的影响。
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引用次数: 4
Successful treatment of iatrogenically developed cutaneous calci-fication by tetracycline in labrador retriever 四环素成功治疗拉布拉多犬医源性皮肤钙化
Pub Date : 1900-01-01 DOI: 10.15761/AHDVS.1000180
Harvima Rj, Halonen P, Pirinen E, Kolehmainen-Harvima Ks, Harvima It
An older of about 11-year-old Labrador retriever dog developed arthrosis especially in the right elbow with a traumatic loosened fragment that was removed in a small operation. The dog was treated first with conventional antiinflammatory drugs, e.g., paracetamol, paracetamol-codeine combination, meloxicam, and finally carprofen-tramadol combination. After these treatments failed to give an adequate response, 40-80 mg methylprednisolone was administered i.m. or s.c. at one to two-month intervals. Skin dermatitis with marked hair loss appeared with clinical appearance of impetigo-like purulent infection seen as multiple lesions. Therefore, cephalexin was administered orally for 4 weeks together with topical antimicrobial treatments with chlorhexidine. In addition, nodular itchy tumors developed. Skin biopsies taken both from areas of dermatitis and nodular lesions revealed marked skin calcification that was treated with oral tetracycline 500 mg bid for 2 months and thereafter 500 mg per day for another 2 months. The itch disappeared in 2 weeks, dermatitis in about 2-3 weeks, and nodular tumors disappeared, and the hair regrew in about 3 months after the end of tetracycline treatment.
一只大约11岁的拉布拉多猎犬患上了关节病,特别是在右肘,创伤性松动碎片在一次小手术中被移除。先用常规抗炎药物治疗,如扑热息痛、扑热息痛-可待因联合用药、美洛昔康、卡洛芬-曲马多联合用药。在这些治疗未能产生足够的反应后,每隔一至两个月给药40-80毫克甲基强的松龙。皮肤皮炎伴明显脱发,临床表现为脓疱样化脓性感染,多处病变。因此,头孢氨苄口服4周,并用氯己定进行局部抗菌治疗。此外,出现结节性瘙痒性肿瘤。皮炎和结节病变部位的皮肤活检显示明显的皮肤钙化,口服四环素500毫克,每次2个月,然后每天500毫克,再持续2个月。四环素治疗结束后2周瘙痒消失,2-3周皮炎消失,结节性肿瘤消失,3个月左右毛发再生。
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引用次数: 0
An in vitro rumen-mimetic continuous cultivation system for evaluating the nutritional value of micropulverized roughage based on volatile fatty acid production 基于挥发性脂肪酸产量评价微粉粗饲料营养价值的体外模拟瘤胃连续培养系统
Pub Date : 1900-01-01 DOI: 10.15761/ahdvs.1000154
H. Agematu, Takehiko Takahashi, Y. Hamano
In order to avoid ruminal acidosis, increasing the amount of energy supplied from roughage (high-cellulose diets) should be considered. The objective is to develop a novel in vitro procedure to evaluate the contribution of micropulverized roughage for which the nutritional value was increased. Ruminal bacteria collected from a Japanese shorthorn cow were continuously cultivated for over 60 days at pH 6.5 using artificial saliva and used to evaluate the nutritional value of 10 or 20 g of dried roughage. The digestion of roughage was monitored using a pH meter to detect the beginning and end of digestion, and the amounts of VFAs (i.e., acetic, propionic, and butyric acids) produced during the digestion were determined by HPLC. The nutritional value (mol-VFAs/kg-substrate; mean ± SE) of microcrystalline cellulose, Italian ryegrass silage, rice straw, alfalfa hay, and micropulverized Japanese cedar were 6.76 ± 0.25, 4.64 ± 0.10, 3.05 ± 0.20, 1.52 ± 0.09, and 0.88 ± 0.10 respectively. By micropulverizing rice straw to an average particle diameter of 20–50 μm, the value of this processed roughage was increased by 135% (4.11 ± 0.27). During cultivation, ruminal bacteria were observed to form biofilms on the surface of feed fragments. The micropulverized roughage became enveloped by these biofilms and was digested by the microbiota growing synergistically within. The digestible nutrients of 20g of roughage were completely digested by feed particleassociated bacteria within approximately 24 h. The microbiota constituted a cellulose metabolic pathway for the conversion of roughage to VFAs. The in vitro procedure measures the accessibility of cellulolytic bacteria to the cellulose of roughage and the amount of digestible cellulose contained in roughage. The procedure is suitable for evaluating the nutritional value of micropulverized roughage and will accelerate the development of it for use in animal feed. *Correspondence to: Hitosi Agematu, Akita National College of Technology, Department of Applied Chemistry, Akita, Japan, Tel: +81-18-847-6063; Fax: +81-18-847-6066; E-mail: agematu@akita-nct.ac.jp
为避免瘤胃酸中毒,应考虑增加粗饲料(高纤维素日粮)的能量供给。目的是开发一种新的体外程序来评估微粉粗饲料对营养价值增加的贡献。在pH为6.5的条件下,利用人工唾液连续培养日本短刺牛瘤胃细菌60天以上,并对10或20 g干粗饲料的营养价值进行评价。用pH计监测粗饲料的消化,检测消化的开始和结束,并通过高效液相色谱法测定消化过程中产生的VFAs(即乙酸、丙酸和丁酸)的数量。营养价值(mol-VFAs/kg-底物;微晶纤维素、意大利黑麦草青贮、水稻秸秆、苜蓿干草和杉木微粉的平均±SE分别为6.76±0.25、4.64±0.10、3.05±0.20、1.52±0.09和0.88±0.10。将秸秆微粉化至平均粒径为20 ~ 50 μm,粗料的加工值提高了135%(4.11±0.27)。在培养过程中,观察到瘤胃细菌在饲料碎片表面形成生物膜。微粉粗料被这些生物膜包裹,并被其中协同生长的微生物群消化。20g粗饲料的可消化营养物质在约24 h内被饲料颗粒相关菌完全消化。该菌群构成了一条将粗饲料转化为VFAs的纤维素代谢途径。体外程序测量纤维素分解细菌对粗饲料中纤维素的可及性以及粗饲料中可消化纤维素的含量。该方法适用于评价微粉粗饲料的营养价值,将加速其在动物饲料中的应用。*通讯对象:日本秋田国立工业大学应用化学系Agematu Hitosi,电话:+81-18-847-6063;传真:+ 81-18-847-6066;电子邮件:agematu@akita-nct.ac.jp
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引用次数: 1
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Animal Husbandry, Dairy and Veterinary Science
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