The data on medicinal plants with antimicrobial properties are reviewed. The study has been carried out on their ability to exhibit antimicrobial and deodorizing effects on Staphylococcus aureus, Echerihia coli, and the feasibility of developing a disinfectant based on tormenta, kalgan, cranberry and vivicle. The antimicrobial effect of these ethanol 40 % tinctures on a plant basis has been established. Among the investigated extracts, the most pronounced effect of Arctostaphylos uva-ursi L. is caused by the death of Staphylococcus aureus with a growth delay of 26 ± 1.7 mm, and Echerihia coli with a growth delay of 29 ± 0.5. The tincture made from Potentilla erecta rhizomata has antimicrobial effect against Staphylococcus aureus with a diameter of growth retardation of 29 ± 2.1 mm and Echerihia coli 27 ± 0.7 mm. Symphytum officinale also has a pronounced antibacterial activity, inhibiting the growth of Staphylococcus aureus to 24 ± 1.7 mm, and Echerihia coli – 17 ± 0.3 mm. The ethanol extract made on the basis of Geranium had the least detrimental effect. The obtained results provide an opportunity for further study of the pharmacological properties of these medicinal plants and the development of a disinfectant based on them. Potentilla erecta rhizomata, a perennial herbaceous plant of the rose family, has attracted significant interest as valuable medicinal raw material due to its high levels of tannins, triterpenoids, tormentosides, and astringent compounds found in its rhizomes and other valuable organic compounds. Preparations based on tormentil provide bactericidal, bitter, anti-inflammatory, expectorant, and choleretic effects. Symphytum officinale, a perennial herbaceous plant belonging to the borage family, has numerous beneficial components in its root, such as alkaloids, allantoin, amino acids, tannins, gallic acid, and digallic acid, among others. The shoots and leaves contain protein, fiber, fat, non-nitrogenous, and extractive substances. Arctostaphylos uva-ursi L is a genus of evergreen, stiff-leaved shrubs in the heath family. The main active compounds are phenolic compounds. Infusions and decoctions of bearberry shoots and leaves exhibit antimicrobial, anti-inflammatory, and diuretic effects. Geranium is a plant species of the Geranium genus in the Geraniaceae family. The plant contains essential oil, flavonoids, carvacrol, borneol, and tannins. Preparations based on geranium have antibacterial, hypotensive, anticonvulsant, and sedative properties. Since the investigated plants contain a large number of diverse bioactive substances with antibacterial properties, the aim of this study is to evaluate the effects of ethanolic extracts on Staphylococcus aureus, a conditionally pathogenic microorganism causing staphylococcal infections, and Escherichia coli, one of the main types of bacteria residing in the lower parts of the mammalian intestine, collectively known as intestinal flora.
{"title":"Analysis of antimicrobial properties of medicinal plants","authors":"A. M. Khyl, S. Peredera","doi":"10.32718/nvlvet11023","DOIUrl":"https://doi.org/10.32718/nvlvet11023","url":null,"abstract":"The data on medicinal plants with antimicrobial properties are reviewed. The study has been carried out on their ability to exhibit antimicrobial and deodorizing effects on Staphylococcus aureus, Echerihia coli, and the feasibility of developing a disinfectant based on tormenta, kalgan, cranberry and vivicle. The antimicrobial effect of these ethanol 40 % tinctures on a plant basis has been established. Among the investigated extracts, the most pronounced effect of Arctostaphylos uva-ursi L. is caused by the death of Staphylococcus aureus with a growth delay of 26 ± 1.7 mm, and Echerihia coli with a growth delay of 29 ± 0.5. The tincture made from Potentilla erecta rhizomata has antimicrobial effect against Staphylococcus aureus with a diameter of growth retardation of 29 ± 2.1 mm and Echerihia coli 27 ± 0.7 mm. Symphytum officinale also has a pronounced antibacterial activity, inhibiting the growth of Staphylococcus aureus to 24 ± 1.7 mm, and Echerihia coli – 17 ± 0.3 mm. The ethanol extract made on the basis of Geranium had the least detrimental effect. The obtained results provide an opportunity for further study of the pharmacological properties of these medicinal plants and the development of a disinfectant based on them. Potentilla erecta rhizomata, a perennial herbaceous plant of the rose family, has attracted significant interest as valuable medicinal raw material due to its high levels of tannins, triterpenoids, tormentosides, and astringent compounds found in its rhizomes and other valuable organic compounds. Preparations based on tormentil provide bactericidal, bitter, anti-inflammatory, expectorant, and choleretic effects. Symphytum officinale, a perennial herbaceous plant belonging to the borage family, has numerous beneficial components in its root, such as alkaloids, allantoin, amino acids, tannins, gallic acid, and digallic acid, among others. The shoots and leaves contain protein, fiber, fat, non-nitrogenous, and extractive substances. Arctostaphylos uva-ursi L is a genus of evergreen, stiff-leaved shrubs in the heath family. The main active compounds are phenolic compounds. Infusions and decoctions of bearberry shoots and leaves exhibit antimicrobial, anti-inflammatory, and diuretic effects. Geranium is a plant species of the Geranium genus in the Geraniaceae family. The plant contains essential oil, flavonoids, carvacrol, borneol, and tannins. Preparations based on geranium have antibacterial, hypotensive, anticonvulsant, and sedative properties. Since the investigated plants contain a large number of diverse bioactive substances with antibacterial properties, the aim of this study is to evaluate the effects of ethanolic extracts on Staphylococcus aureus, a conditionally pathogenic microorganism causing staphylococcal infections, and Escherichia coli, one of the main types of bacteria residing in the lower parts of the mammalian intestine, collectively known as intestinal flora.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48520903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Krivenko, I. Rublenko, S. Rublenko, V. Koziy, R. Shaganenko, O. Gorbatiuk
Currently, the most common ophthalmic pathology in cats is inflammatory processes. Pathologies are observed frequently and regardless of breed, sex, and age. The causes of the disease are the prevalence of infections affecting the visual analyzer, inadequate care of their pets by the owners, untimely visits to a veterinarian, etc. It is essential to diagnose the cause of inflammation of the ocular mucosa in time, as knowing the etiology of the disease is necessary to prescribe effective treatment, which can lead to complications. Bacteriological tests play an important role, allowing us not only to identify the pathogen but also to determine its sensitivity or resistance to a particular drug, to prescribe effective treatment in time, to reduce the economic costs of treatment, the treatment period, etc. Most of the microorganisms that can be found in the conjunctival sac are non-pathogenic, although some of them are opportunistic. The study was conducted at the VetExpert veterinary center on cats of all ages and sexes with signs of conjunctivitis during 2022–2023. Out of 473 cats examined, cases of eye disease were detected in 105 cats or 22.1 % of the total number of ophthalmopathology cases. According to the results of the research, it was found that conjunctivitis was caused by Staphylococcus aureus, Staphylococcus epidermidis, Nonhaemolytic Streptococcus, and Pseudomonas aeruginosa. In treating conjunctivitis of bacterial etiology, topical application of the drug and systemic antibiotic therapy should be prescribed. In treating cats with bacterial conjunctivitis, systemic drugs, and antibiotic therapy should be prescribed. Antibiotics and antibacterial drugs should be prescribed only based on the results of bacteriological examination and antibioticogramа.
{"title":"Antibiotic resistance of microflora to drugs in case of conjunctivitis of cats of bacterial origin","authors":"N. Krivenko, I. Rublenko, S. Rublenko, V. Koziy, R. Shaganenko, O. Gorbatiuk","doi":"10.32718/nvlvet11004","DOIUrl":"https://doi.org/10.32718/nvlvet11004","url":null,"abstract":"Currently, the most common ophthalmic pathology in cats is inflammatory processes. Pathologies are observed frequently and regardless of breed, sex, and age. The causes of the disease are the prevalence of infections affecting the visual analyzer, inadequate care of their pets by the owners, untimely visits to a veterinarian, etc. It is essential to diagnose the cause of inflammation of the ocular mucosa in time, as knowing the etiology of the disease is necessary to prescribe effective treatment, which can lead to complications. Bacteriological tests play an important role, allowing us not only to identify the pathogen but also to determine its sensitivity or resistance to a particular drug, to prescribe effective treatment in time, to reduce the economic costs of treatment, the treatment period, etc. Most of the microorganisms that can be found in the conjunctival sac are non-pathogenic, although some of them are opportunistic. The study was conducted at the VetExpert veterinary center on cats of all ages and sexes with signs of conjunctivitis during 2022–2023. Out of 473 cats examined, cases of eye disease were detected in 105 cats or 22.1 % of the total number of ophthalmopathology cases. According to the results of the research, it was found that conjunctivitis was caused by Staphylococcus aureus, Staphylococcus epidermidis, Nonhaemolytic Streptococcus, and Pseudomonas aeruginosa. In treating conjunctivitis of bacterial etiology, topical application of the drug and systemic antibiotic therapy should be prescribed. In treating cats with bacterial conjunctivitis, systemic drugs, and antibiotic therapy should be prescribed. Antibiotics and antibacterial drugs should be prescribed only based on the results of bacteriological examination and antibioticogramа.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45519661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Snails are a delicacy that people have been eating for centuries. Products made from this meat contain few calories per 100 grams and fill the human body with useful elements. They can be prepared by cooking, namely escargot with various fillings (Burgundy snails, Catalan snails, Asian snails, snails with porcini mushrooms), and also, now a new type of product containing m gastropod mollusks – puffs with snails. If determining the quality and nutritional value of meat, it is necessary to determine indicators, if there is doubt about its suitability, which characterize the chemical composition, freshness, as well as the determination of microbiological contamination. The purpose of this work was to determine the features and practical aspects of examination of gastropod molluscs – snails. The studies highlighted in the scientific publications of Ukrainian scientists are significant, but they relate to the determination of the degree of freshness of snail meat by various methods and the organization of snail farming in various ways. Snails are a delicacy, dietary product of high commercial value and belong to the few types of food products. They are subject to mandatory veterinary and sanitary examination. A special feature of examination of snails is microbiological and bacteriological types of control to establish their general safety. In the course of research, first of all, the organoleptic indicators of molluscs, which reflect their freshness, are determined. Next, they conduct research on the presence of pathogenic microflora, determine the presence of radionuclides, conduct bacteriological research. In addition to the mollusk itself, the water with which they are irrigated, as well as the soil – the permanent habitat of snails – are also examined for the presence of helminth eggs. It was established that it is necessary to carry out laboratory control of each batch of snail meat for the content of microorganisms, such as MAFAnM, BGKP (coliforms), coagulase-positive staphylococcus (S. aureus), bacteria of the genus Proteus, pathogenic microorganisms, including salmonella, Listeria monocytogenes, radionuclides – Cs-137 and Sr-90 and helminths (nematodes).
{"title":"Features of snail expertise","authors":"I. Danilova, T. Danilova","doi":"10.32718/nvlvet11006","DOIUrl":"https://doi.org/10.32718/nvlvet11006","url":null,"abstract":"Snails are a delicacy that people have been eating for centuries. Products made from this meat contain few calories per 100 grams and fill the human body with useful elements. They can be prepared by cooking, namely escargot with various fillings (Burgundy snails, Catalan snails, Asian snails, snails with porcini mushrooms), and also, now a new type of product containing m gastropod mollusks – puffs with snails. If determining the quality and nutritional value of meat, it is necessary to determine indicators, if there is doubt about its suitability, which characterize the chemical composition, freshness, as well as the determination of microbiological contamination. The purpose of this work was to determine the features and practical aspects of examination of gastropod molluscs – snails. The studies highlighted in the scientific publications of Ukrainian scientists are significant, but they relate to the determination of the degree of freshness of snail meat by various methods and the organization of snail farming in various ways. Snails are a delicacy, dietary product of high commercial value and belong to the few types of food products. They are subject to mandatory veterinary and sanitary examination. A special feature of examination of snails is microbiological and bacteriological types of control to establish their general safety. In the course of research, first of all, the organoleptic indicators of molluscs, which reflect their freshness, are determined. Next, they conduct research on the presence of pathogenic microflora, determine the presence of radionuclides, conduct bacteriological research. In addition to the mollusk itself, the water with which they are irrigated, as well as the soil – the permanent habitat of snails – are also examined for the presence of helminth eggs. It was established that it is necessary to carry out laboratory control of each batch of snail meat for the content of microorganisms, such as MAFAnM, BGKP (coliforms), coagulase-positive staphylococcus (S. aureus), bacteria of the genus Proteus, pathogenic microorganisms, including salmonella, Listeria monocytogenes, radionuclides – Cs-137 and Sr-90 and helminths (nematodes).","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46316555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although teat disinfection before milking has advantages, some producers believe the use of disinfectants to be unprofitable compared to qualitative teat processing using warm water with skin drying using paper towels. The aim of the work was to compare the effect of pre-milking treatment of cows' udders with Oxy Foam and Pre-Dip on the reduction of mastitis pathogens on the skin of teats cows on large dairy farms. The study was conducted in the winter-spring period on two dairy plants with a stable-free range cow breeding system. In the first group of animals: the right anterior and the right posterior teats of the udder were processed using disinfectant Oxy Foam and the left anterior and the left posterior teats were washed using warm water (control). Pre-milking treatment of the studied teats using disinfectant included foam application, keeping it for 30 seconds, and further wiping dry using disposable paper towels. In the second group of animals: the right teats were treated with Pre-Dip with the help of a cup by immersion with a subsequent exposure of 15 seconds. Swish from the skin of udder teats were taken using sterile tampons before the processing before milking and repeatedly after the end of the milking process. The ordinary treatment using warm water and drying using paper towels was found to reduce the number of microorganisms on the teat skin by 2.5 times (Р ≤ 0.001), while the use of disinfectant Oxy Foam – by 6.4 times (Р ≤ 0.001), and Pre-Dip – 4.8 times (Р ≤ 0.001). Herewith, treatment with water reduced the amount of hemolytic staphylococci on the teat skin – by 1.9 times (Р ≤ 0.01), disinfection using Oxy Foam – by 4.4 times (Р ≤ 0.001) to 13.2 ± 3.14 CFU/cm3 of the wash, and with Pre-Dip – 4 times (Р ≤ 0.001) to 14.6 ± 3.05 CFU/cm3 of wash. After using warm water, the number of pathogenic streptococcus reduced by 1.8 times (Р ≤ 0.01), and by 2.5 times (Р ≤ 0.001), when using Oxy Foam and Pre-Dip – 2.1 times (Р ≤ 0.001) to 7.5–8.9 CFU/cm3 of the wash. Microorganisms of Escherichia kind were well-removed with the use of ordinary -pre-milking treatment and were distinguished from the cow teat skin in single cases in the number of only a few cultures. Therefore, Oxy Foam based on hydrogen peroxide and lactic acid turned out to be a more effective means for treating the udder of cows before milking, compared to Pre-Dip with a content of 0.15 % of free iodine. Further research will be aimed at creating new domestic ecologically safe means for the pre-milking treatment of cow udders.
{"title":"Comparison of the effectiveness of the means “Oxy Foam” and “Pre-Dip” in reducing the causative agents of mastitis on the skin of cows' teats","authors":"Y. Perkiy, T. Trukhanovych","doi":"10.32718/nvlvet11015","DOIUrl":"https://doi.org/10.32718/nvlvet11015","url":null,"abstract":"Although teat disinfection before milking has advantages, some producers believe the use of disinfectants to be unprofitable compared to qualitative teat processing using warm water with skin drying using paper towels. The aim of the work was to compare the effect of pre-milking treatment of cows' udders with Oxy Foam and Pre-Dip on the reduction of mastitis pathogens on the skin of teats cows on large dairy farms. The study was conducted in the winter-spring period on two dairy plants with a stable-free range cow breeding system. In the first group of animals: the right anterior and the right posterior teats of the udder were processed using disinfectant Oxy Foam and the left anterior and the left posterior teats were washed using warm water (control). Pre-milking treatment of the studied teats using disinfectant included foam application, keeping it for 30 seconds, and further wiping dry using disposable paper towels. In the second group of animals: the right teats were treated with Pre-Dip with the help of a cup by immersion with a subsequent exposure of 15 seconds. Swish from the skin of udder teats were taken using sterile tampons before the processing before milking and repeatedly after the end of the milking process. The ordinary treatment using warm water and drying using paper towels was found to reduce the number of microorganisms on the teat skin by 2.5 times (Р ≤ 0.001), while the use of disinfectant Oxy Foam – by 6.4 times (Р ≤ 0.001), and Pre-Dip – 4.8 times (Р ≤ 0.001). Herewith, treatment with water reduced the amount of hemolytic staphylococci on the teat skin – by 1.9 times (Р ≤ 0.01), disinfection using Oxy Foam – by 4.4 times (Р ≤ 0.001) to 13.2 ± 3.14 CFU/cm3 of the wash, and with Pre-Dip – 4 times (Р ≤ 0.001) to 14.6 ± 3.05 CFU/cm3 of wash. After using warm water, the number of pathogenic streptococcus reduced by 1.8 times (Р ≤ 0.01), and by 2.5 times (Р ≤ 0.001), when using Oxy Foam and Pre-Dip – 2.1 times (Р ≤ 0.001) to 7.5–8.9 CFU/cm3 of the wash. Microorganisms of Escherichia kind were well-removed with the use of ordinary -pre-milking treatment and were distinguished from the cow teat skin in single cases in the number of only a few cultures. Therefore, Oxy Foam based on hydrogen peroxide and lactic acid turned out to be a more effective means for treating the udder of cows before milking, compared to Pre-Dip with a content of 0.15 % of free iodine. Further research will be aimed at creating new domestic ecologically safe means for the pre-milking treatment of cow udders.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48239139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal welfare is a multifaceted issue of international and state policy, which requires a socio-economic, religious, and cultural approach, must consider international trade features, and must correspond to the vector of development of modern society. The new global plans and challenges of the animal protection community are to help feed the world healthily and sustainably that takes face the needs of people and is safe for animals. The protection and provision of animal welfare depend on the species differences of agricultural and productive animals, which are determined by physiological, biochemical, and behavioral factors, as well as on the differences between climatic zones, which are analyzed in the article. Modern animal husbandry systems must meet the latest knowledge about stress and be based on the science of ethology, comply with the concept of the “Five freedoms” of the welfare of productive animals, based on which a system of clear standards of animal welfare has been developed and implemented as a component of national and international public policy, commerce, and trade. However, animal welfare science continues to develop and deepen our understanding of the interaction between humankind and agricultural species by integrating ethological, physiological, and biochemical methods, methods of assessing the genotype, and its interaction with the environment into the systems for assessing the quality of animal welfare. The assessment includes measuring the artificial impact of farm conditions, microclimate, etc., on the general animal’s welfare and the interaction between individuals in the middle of a limited population. An important factor that has affected animal welfare recently is the restrictions introduced due to the COVID-19 pandemic, which affected feed supply chains, animal transportation, and limited access to high-quality veterinary services. In recent years, Ukraine has been actively implementing the international experience of animal welfare regulation into domestic legal acts. It continues harmonizing with the European Union's legal system, which covers animals' welfare during their keeping, transportation, and slaughter. A key element on the way to the practical application of the principles of animal welfare is the training of specialists – doctors of veterinary medicine, who would be aware of the importance and necessity of such approaches in their daily professional activities.
{"title":"Modern approaches to ensure the welfare of productive animals","authors":"Y. Veremchuk","doi":"10.32718/nvlvet11007","DOIUrl":"https://doi.org/10.32718/nvlvet11007","url":null,"abstract":"Animal welfare is a multifaceted issue of international and state policy, which requires a socio-economic, religious, and cultural approach, must consider international trade features, and must correspond to the vector of development of modern society. The new global plans and challenges of the animal protection community are to help feed the world healthily and sustainably that takes face the needs of people and is safe for animals. The protection and provision of animal welfare depend on the species differences of agricultural and productive animals, which are determined by physiological, biochemical, and behavioral factors, as well as on the differences between climatic zones, which are analyzed in the article. Modern animal husbandry systems must meet the latest knowledge about stress and be based on the science of ethology, comply with the concept of the “Five freedoms” of the welfare of productive animals, based on which a system of clear standards of animal welfare has been developed and implemented as a component of national and international public policy, commerce, and trade. However, animal welfare science continues to develop and deepen our understanding of the interaction between humankind and agricultural species by integrating ethological, physiological, and biochemical methods, methods of assessing the genotype, and its interaction with the environment into the systems for assessing the quality of animal welfare. The assessment includes measuring the artificial impact of farm conditions, microclimate, etc., on the general animal’s welfare and the interaction between individuals in the middle of a limited population. An important factor that has affected animal welfare recently is the restrictions introduced due to the COVID-19 pandemic, which affected feed supply chains, animal transportation, and limited access to high-quality veterinary services. In recent years, Ukraine has been actively implementing the international experience of animal welfare regulation into domestic legal acts. It continues harmonizing with the European Union's legal system, which covers animals' welfare during their keeping, transportation, and slaughter. A key element on the way to the practical application of the principles of animal welfare is the training of specialists – doctors of veterinary medicine, who would be aware of the importance and necessity of such approaches in their daily professional activities.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44529520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There are practically no disinfectants on the market of veterinary drugs that fully meet the requirements. This is due to the significant diversity and peculiarity of the structure of microorganisms and their ability to form resistance during long-term use of the same disinfectants. This is one of the reasons for the active search for effective active substances, the development of new drugs, and their registration, contributing to quite intensive changes in the range of disinfectants on the market. The work aimed to analyze the production, determine the share of domestic and imported disinfectants, determine the main active substances, and the range of disinfectants registered in Ukraine. The research material was a list of disinfectants registered in Ukraine used in animal husbandry, veterinary and humane medicine, and the food industry. It was established that from 2018 to 2022, 66 domestic and imported disinfectants were registered in Ukraine. Imported disinfectants are produced mainly in Great Britain, France, Belgium, Spain, Germany, Israel, Slovenia, Denmark, and the Netherlands. From among domestic companies, PP “Kronos Agro”, LLC “BioTestLab”, LLC “SANFORT-P”, LLC “Brovapharma”, LLC “Ukrvetprompostach”, LLC “Ukrainian-Polish joint enterprise “ZVK”, LLC “VetAgro” are engaged in the production of disinfectants, LLC “Inter-Syntez”, LLC “Ukrainian Chemical Technologies LTD”, LLC “GREENPAKS”, LLC NVK “Globus”, LLC “Vetsyntez”, PF “Termit”, LLC “ABM-Trade”, LLC “Dezsystema”, LLC “Tandem-2002”, NVPP “DEZO” and LLC “UKRTEK KO”. It was determined that manufacturers often use chlorine and oxygen as active ingredients, and the number of products with one active ingredient is relatively insignificant. Most often, the active substance of modern disinfectants is quaternary ammonium salts in combination with aldehydes. Their number is 33.4 % of all registered means. This means combining QAS and acids and QAS and oxygen compounds takes 3.2 times less time. Means containing other combinations of active substances are from 1.5 to 4.6 %. It was established that the leader in the production of imported “cleansing probiotics” (PIP – Probiotics In Progress) is the British company “Ingenious Probiotics”, which produces 66.7 % of registered products. Three companies produce this kind of disinfectant in our country; their assortment includes 23 names, and the largest share belongs to the company “Sirion”.
市场上几乎没有完全符合要求的兽药消毒剂。这是由于微生物结构的显著多样性和特殊性以及它们在长期使用同一消毒剂期间形成耐药性的能力。这是积极寻找有效活性物质、开发新药及其注册的原因之一，导致市场上消毒剂的范围发生了相当大的变化。这项工作旨在分析生产情况，确定国产和进口消毒剂的份额，确定主要活性物质，以及在乌克兰注册的消毒剂的范围。研究材料是在乌克兰注册的用于畜牧业、兽医和人道医学以及食品工业的消毒剂清单。据了解，2018年至2022年，乌克兰共登记了66种国产和进口消毒剂。进口消毒剂主要在英国、法国、比利时、西班牙、德国、以色列、斯洛文尼亚、丹麦、荷兰等国家生产。从国内公司中，PP“Kronos Agro”，LLC“BioTestLab”，LLC“SANFORT-P”，LLC“Brovapharma”，LLC“Ukrvetprompostach”，LLC“乌克兰-波兰合资企业”ZVK”，LLC“VetAgro”从事消毒剂生产，LLC“Inter-Syntez”，LLC“乌克兰化学技术有限公司”，LLC“GREENPAKS”，LLC NVK“Globus”，LLC“Vetsyntez”，PF“Termit”，LLC“ABM-Trade”，LLC“Dezsystema”，LLC“Tandem-2002”，NVPP“DEZO”和LLC“UKRTEK KO”。结果表明，生产厂家经常使用氯和氧作为有效成分，而使用一种有效成分的产品数量相对较少。大多数情况下，现代消毒剂的活性物质是季铵盐与醛的结合。他们的数量占所有登记平均数的33.4%。这意味着QAS与酸、QAS与氧化合物结合的时间减少了3.2倍。含有其他活性物质组合的平均值为1.5%至4.6%。确定了进口“清洁益生菌”(PIP - probiotics in Progress)生产的领导者是英国公司“Ingenious probiotics”，其生产的注册产品占66.7%。我国有三家公司生产这种消毒剂;他们的分类包括23个名字，最大的份额属于“Sirion”公司。
{"title":"Retrospective analysis of production of main active ingredients and assortment of disinfectants in Ukraine","authors":"V. Myronchuk, R. Peleno","doi":"10.32718/nvlvet11011","DOIUrl":"https://doi.org/10.32718/nvlvet11011","url":null,"abstract":"There are practically no disinfectants on the market of veterinary drugs that fully meet the requirements. This is due to the significant diversity and peculiarity of the structure of microorganisms and their ability to form resistance during long-term use of the same disinfectants. This is one of the reasons for the active search for effective active substances, the development of new drugs, and their registration, contributing to quite intensive changes in the range of disinfectants on the market. The work aimed to analyze the production, determine the share of domestic and imported disinfectants, determine the main active substances, and the range of disinfectants registered in Ukraine. The research material was a list of disinfectants registered in Ukraine used in animal husbandry, veterinary and humane medicine, and the food industry. It was established that from 2018 to 2022, 66 domestic and imported disinfectants were registered in Ukraine. Imported disinfectants are produced mainly in Great Britain, France, Belgium, Spain, Germany, Israel, Slovenia, Denmark, and the Netherlands. From among domestic companies, PP “Kronos Agro”, LLC “BioTestLab”, LLC “SANFORT-P”, LLC “Brovapharma”, LLC “Ukrvetprompostach”, LLC “Ukrainian-Polish joint enterprise “ZVK”, LLC “VetAgro” are engaged in the production of disinfectants, LLC “Inter-Syntez”, LLC “Ukrainian Chemical Technologies LTD”, LLC “GREENPAKS”, LLC NVK “Globus”, LLC “Vetsyntez”, PF “Termit”, LLC “ABM-Trade”, LLC “Dezsystema”, LLC “Tandem-2002”, NVPP “DEZO” and LLC “UKRTEK KO”. It was determined that manufacturers often use chlorine and oxygen as active ingredients, and the number of products with one active ingredient is relatively insignificant. Most often, the active substance of modern disinfectants is quaternary ammonium salts in combination with aldehydes. Their number is 33.4 % of all registered means. This means combining QAS and acids and QAS and oxygen compounds takes 3.2 times less time. Means containing other combinations of active substances are from 1.5 to 4.6 %. It was established that the leader in the production of imported “cleansing probiotics” (PIP – Probiotics In Progress) is the British company “Ingenious Probiotics”, which produces 66.7 % of registered products. Three companies produce this kind of disinfectant in our country; their assortment includes 23 names, and the largest share belongs to the company “Sirion”.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44447761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Zaika, T. Kot, S. Guralska, Z. Khomenko, A. Dubovyi
Heat stress as a result of hyperthermia and dehydration of the body due to natural or artificial environmental factors negatively affects the well-being of birds. Heat stress factor can lead to significant economic losses in poultry farms due to a decrease in egg production, egg fertilization, hatching and viability of young animals, morphological indicators of eggs, an increase in bird mortality during maintenance and transportation, the occurrence of immunosuppression and metabolic disorders. Pathomorphological changes in the internal organs of birds under heat stress are classified as degenerative changes, necrosis, circulatory disorders, changes in tissue fluid, growth disorders, and inflammation. The adrenal gland affects the number of functional and metabolic processes in the body of birds, its hormones ensure its resistance to heat stress. In the current global warming climate, the study of the morphology of the adrenal gland of birds due to the action of a thermal stress factor is an urgent problem of poultry farming, since its solution contributes to the scientific justification of technologies for growing, using and treating birds. The aim of the study was to identify morphological changes in the adrenal gland of white leghorn chickens aged 3 months under heat stress. Anatomical, morphometric, microscopic and statistical research methods were used in the course of the study. It was found that the indicators of absolute mass and linear dimensions of the adrenal glands of chickens under heat stress increased relative to such indicators in clinically healthy chickens. Microscopically, circulatory disorders (edema, hyperemia, hemorrhages, congestion, thrombosis) were recorded in the adrenal glands of chickens, which on the 4th day of exposure to the heat stress factor were accompanied by activation of regenerative processes (an increase in the number of fibroblasts under the capsule) and secretory activity of endocrinocytes (an increase in the size of interrenal and suprarenal cells). Intense and prolonged stress of the adrenal glands of chickens caused by heat stress led to the withdrawal of their plastic and energy resources, and as a result caused the development of adrenal deficiency on Day 8, which was observed destructive and dystrophic changes in the interrenal and supraranal cells. The obtained data can be used to develop morphofunctional diagnostic and prognostic criteria for the state of both the adrenal glands and the avian organ as a whole under heat stress. In the future of further research-to study the features of the content and localization of nucleic acids, proteins, carbohydrates, lipids in the adrenal glands of chickens under heat stress.
{"title":"Morphological changes in the adrenal glands of chickens under heat stress","authors":"S. Zaika, T. Kot, S. Guralska, Z. Khomenko, A. Dubovyi","doi":"10.32718/nvlvet11010","DOIUrl":"https://doi.org/10.32718/nvlvet11010","url":null,"abstract":"Heat stress as a result of hyperthermia and dehydration of the body due to natural or artificial environmental factors negatively affects the well-being of birds. Heat stress factor can lead to significant economic losses in poultry farms due to a decrease in egg production, egg fertilization, hatching and viability of young animals, morphological indicators of eggs, an increase in bird mortality during maintenance and transportation, the occurrence of immunosuppression and metabolic disorders. Pathomorphological changes in the internal organs of birds under heat stress are classified as degenerative changes, necrosis, circulatory disorders, changes in tissue fluid, growth disorders, and inflammation. The adrenal gland affects the number of functional and metabolic processes in the body of birds, its hormones ensure its resistance to heat stress. In the current global warming climate, the study of the morphology of the adrenal gland of birds due to the action of a thermal stress factor is an urgent problem of poultry farming, since its solution contributes to the scientific justification of technologies for growing, using and treating birds. The aim of the study was to identify morphological changes in the adrenal gland of white leghorn chickens aged 3 months under heat stress. Anatomical, morphometric, microscopic and statistical research methods were used in the course of the study. It was found that the indicators of absolute mass and linear dimensions of the adrenal glands of chickens under heat stress increased relative to such indicators in clinically healthy chickens. Microscopically, circulatory disorders (edema, hyperemia, hemorrhages, congestion, thrombosis) were recorded in the adrenal glands of chickens, which on the 4th day of exposure to the heat stress factor were accompanied by activation of regenerative processes (an increase in the number of fibroblasts under the capsule) and secretory activity of endocrinocytes (an increase in the size of interrenal and suprarenal cells). Intense and prolonged stress of the adrenal glands of chickens caused by heat stress led to the withdrawal of their plastic and energy resources, and as a result caused the development of adrenal deficiency on Day 8, which was observed destructive and dystrophic changes in the interrenal and supraranal cells. The obtained data can be used to develop morphofunctional diagnostic and prognostic criteria for the state of both the adrenal glands and the avian organ as a whole under heat stress. In the future of further research-to study the features of the content and localization of nucleic acids, proteins, carbohydrates, lipids in the adrenal glands of chickens under heat stress.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47252724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Yevstafieva, O. Kasianenko, J. Negreba, B. Kyrychko, V. Levytska, K. Havryk
The genus Trichuris is a well-known group of nematodes whose species parasitize humans and many domestic animals, including carnivores, cattle, small ruminants and pigs. These parasites in the embryonic stages of development are quite resistant to adverse environmental conditions, which poses a threat of significant spread of trichurosis in livestock farms. The aim of the work was to determine the ovicidal effectiveness of the Hermecid-VS disinfectant (Vetsintez LLC, Ukraine) on the eggs of Trichuris spp. nematodes isolated from cattle under experimental conditions. To determine the densivasive activity, the disinfectant was used in 0.1 %, 0.25 % and 0.5 % concentrations for different exposures – 10, 30 and 60 minutes. The test-culture of trichurises eggs was obtained from the feces of sick animals using the flotation method and subsequent washing in a physiological solution. According to the research results, it was established that the Hermecid-VS preparation has a high level of ovicidal efficiency against the eggs of Trichuris spp., which parasitize cattle, at a concentration of 0.25 % for exposure of 60 minutes and 0.5 % for exposures of 10–60 minutes . Under these regimes, the disinfectant ensured 100 % death of trichurises eggs at various stages of their development in vitro. Also, a high level of ovicidal efficiency was found when using Hermecid-VS at a concentration of 0.25 % for 10 min (90.91 %) and 30 min (96.59 %) exposures. A satisfactory level of ovicidal efficiency was obtained using the agent at a concentration of 0.1 % for exposures of 10, 30 and 60 minutes, where the indicators of disinfestation activity were at the level of 63.64 %, 68.18 % and 76.14 %, respectively. The obtained research results allow us to recommend the Hermecid-VS disinfectant in 0.25 % concentration for 60-minute exposure and 0.5 % concentration for 10-minute exposure for disinfestation as a component of measures to combat and prevent cattle trichurosis, as well as with for the purpose of maintaining veterinary-sanitary and epizootic well-being in livestock farms.
{"title":"Ovicidal effectiveness of Hermecid-VS disinfectant against Trichuris spp. nematode eggs isolated from cattle","authors":"V. Yevstafieva, O. Kasianenko, J. Negreba, B. Kyrychko, V. Levytska, K. Havryk","doi":"10.32718/nvlvet11018","DOIUrl":"https://doi.org/10.32718/nvlvet11018","url":null,"abstract":"The genus Trichuris is a well-known group of nematodes whose species parasitize humans and many domestic animals, including carnivores, cattle, small ruminants and pigs. These parasites in the embryonic stages of development are quite resistant to adverse environmental conditions, which poses a threat of significant spread of trichurosis in livestock farms. The aim of the work was to determine the ovicidal effectiveness of the Hermecid-VS disinfectant (Vetsintez LLC, Ukraine) on the eggs of Trichuris spp. nematodes isolated from cattle under experimental conditions. To determine the densivasive activity, the disinfectant was used in 0.1 %, 0.25 % and 0.5 % concentrations for different exposures – 10, 30 and 60 minutes. The test-culture of trichurises eggs was obtained from the feces of sick animals using the flotation method and subsequent washing in a physiological solution. According to the research results, it was established that the Hermecid-VS preparation has a high level of ovicidal efficiency against the eggs of Trichuris spp., which parasitize cattle, at a concentration of 0.25 % for exposure of 60 minutes and 0.5 % for exposures of 10–60 minutes . Under these regimes, the disinfectant ensured 100 % death of trichurises eggs at various stages of their development in vitro. Also, a high level of ovicidal efficiency was found when using Hermecid-VS at a concentration of 0.25 % for 10 min (90.91 %) and 30 min (96.59 %) exposures. A satisfactory level of ovicidal efficiency was obtained using the agent at a concentration of 0.1 % for exposures of 10, 30 and 60 minutes, where the indicators of disinfestation activity were at the level of 63.64 %, 68.18 % and 76.14 %, respectively. The obtained research results allow us to recommend the Hermecid-VS disinfectant in 0.25 % concentration for 60-minute exposure and 0.5 % concentration for 10-minute exposure for disinfestation as a component of measures to combat and prevent cattle trichurosis, as well as with for the purpose of maintaining veterinary-sanitary and epizootic well-being in livestock farms.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47172272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the process of cryopreservation of sperm, there are violations of the ultrastructure of spermatozoa, which causes biochemical and functional changes in them. To protect spermatozoa from the negative effects of low temperatures, cryopreservation media are used, to which trace elements are added. Recently, nanoforms of trace elements have been obtained in Ukraine and experiments have been started to study their effect on the animal body. The aim of the work was to find out the effect of adding nanosuccinate of Mn, Zn and Cu to the medium for cryopreservation of ram sperm on the quality and fertilizing ability of sperm. The experiment was conducted on six clinically healthy breeder rams, aged 2–4 years. After evaluating the ejaculate from six rams aged 2–4 years, the Texel breed was divided into control and experimental groups. Control sperm samples were diluted with lactose-yolk-tris-citrate-glycerin medium (LYTCGM). Nanosuccinates of microelements were added to the medium in test samples of ram sperm in the following doses: Zn and Mn – 2.5, 5.0 and 7.5 μg/l, Cu – 1.25, 2.5 and 3.75 μg/l. Diluted sperm was packaged in straws, equilibrated for 2.5 hours and frozen. After thawing of sperm, motility, percentage of damaged spermatozoa, their survival, activity of succinate dehydrogenase (SDH) and cytochrome oxidase (CO) in sperm were determined. A dose-dependent effect of Mn, Zn, and Cu nanocitrates upon their addition to LYTCGM was established. The addition of Mn and Zn nanosuccinate at a dose of 5.0 μg/l to LYTCGM probably increases the motility of ram spermatozoa after thawing, and also reduces the percentage of spermatozoa with morphological disorders. Addition of Cu nanosuccinate in increasing doses significantly reduces spermatozoa motility in thawed ram semen, simultaneously increasing the percentage of degenerate spermatozoa. After the addition of Mn and Zn nanosuccinate at a dose of 5.0 μg/l LYTCGM, the survival rate of ram spermatozoa is probably increased, and the addition of Cu nanosuccinate in increasing doses significantly reduces the survival time of germ cells. Addition of Mn and Zn nanosuccinate at a dose of 5.0 μg/l to LYTCGM probably increases the activity of SDH and CO in sperm after thawing, and the addition of Cu nanosuccinate in increasing doses significantly reduces the activity of these enzymes. The addition of Mn and Zn nanosuccinate in lower (2.5 μg/l) and higher (7.5 μg/l) doses did not significantly affect the motility, morphological disorders and survival of spermatozoa, as well as the activity of SDH and CO in them.
{"title":"The quality and fertilizing ability of sperm after the addition of nanosuccinates of Mn, Cu, Zn to the medium for cryopreservation of ram sperm","authors":"O. Sharan, V. Stefanyk, D. Ostapiv","doi":"10.32718/nvlvet11024","DOIUrl":"https://doi.org/10.32718/nvlvet11024","url":null,"abstract":"In the process of cryopreservation of sperm, there are violations of the ultrastructure of spermatozoa, which causes biochemical and functional changes in them. To protect spermatozoa from the negative effects of low temperatures, cryopreservation media are used, to which trace elements are added. Recently, nanoforms of trace elements have been obtained in Ukraine and experiments have been started to study their effect on the animal body. The aim of the work was to find out the effect of adding nanosuccinate of Mn, Zn and Cu to the medium for cryopreservation of ram sperm on the quality and fertilizing ability of sperm. The experiment was conducted on six clinically healthy breeder rams, aged 2–4 years. After evaluating the ejaculate from six rams aged 2–4 years, the Texel breed was divided into control and experimental groups. Control sperm samples were diluted with lactose-yolk-tris-citrate-glycerin medium (LYTCGM). Nanosuccinates of microelements were added to the medium in test samples of ram sperm in the following doses: Zn and Mn – 2.5, 5.0 and 7.5 μg/l, Cu – 1.25, 2.5 and 3.75 μg/l. Diluted sperm was packaged in straws, equilibrated for 2.5 hours and frozen. After thawing of sperm, motility, percentage of damaged spermatozoa, their survival, activity of succinate dehydrogenase (SDH) and cytochrome oxidase (CO) in sperm were determined. A dose-dependent effect of Mn, Zn, and Cu nanocitrates upon their addition to LYTCGM was established. The addition of Mn and Zn nanosuccinate at a dose of 5.0 μg/l to LYTCGM probably increases the motility of ram spermatozoa after thawing, and also reduces the percentage of spermatozoa with morphological disorders. Addition of Cu nanosuccinate in increasing doses significantly reduces spermatozoa motility in thawed ram semen, simultaneously increasing the percentage of degenerate spermatozoa. After the addition of Mn and Zn nanosuccinate at a dose of 5.0 μg/l LYTCGM, the survival rate of ram spermatozoa is probably increased, and the addition of Cu nanosuccinate in increasing doses significantly reduces the survival time of germ cells. Addition of Mn and Zn nanosuccinate at a dose of 5.0 μg/l to LYTCGM probably increases the activity of SDH and CO in sperm after thawing, and the addition of Cu nanosuccinate in increasing doses significantly reduces the activity of these enzymes. The addition of Mn and Zn nanosuccinate in lower (2.5 μg/l) and higher (7.5 μg/l) doses did not significantly affect the motility, morphological disorders and survival of spermatozoa, as well as the activity of SDH and CO in them.","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43717731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article presents a study of glutathione peroxidase (GPO) isozymes content in ovarian tissue under various physiological and pathological states of cow gonads. The ovarian tissue of 12 cows in different physiological conditions were selected for research, from which homogenates were prepared. The content of isozymes was determined in the supernatant after centrifugation by electrophoresis. After electrophoresis in a 7.5 % polyacrylamide gel and specific staining of the plates according to Lin C. L. and Weydert C. J., 5 bands of catalytically active isozymes of the enzyme (GPO1, GPO2, GPO3, GPO4, and GPO5) were established. They differed in electrophoretic mobility, intensity, and area of band staining. It has been proven that depending on the physiological state of the ovaries, the content of individual isozymes in the gonad tissue changed. In particular, it was found that the content of GPO1 for “late corpus luteum” and “follicular growth” was almost the same, and was in the range of 14.2–15.6 %, and for “early corpus luteum” it was 8.7–10.1 % lower (Р < 0.01). The content of GPO2 was high in “early corpus luteum” (19.9 ± 6.42 %), and in “late corpus luteum” and “follicular growth” it was lower on 11.0–13.2 % (Р < 0.01). The content of GPO3 during “follicular growth” and “early corpus luteum” was within 50.1–51.2 %, and during “late” it decreased on 4.5–5.6 %. According to changes in the physiological state of the ovary in the tissue of “late corpus luteum”, the content of GPO4 was the highest (26.0 ± 2.40 %), and under “follicular growth” and “early corpus luteum” it was lower on 10.2 (Р < 0.05) and 12.9 % (Р < 0.01), respectively. A high content of GPO5 was established in the ovarian tissue of “follicular growth” (10.8 ± 2.00 %), which tended to decrease by 2.8 % and by 4.8 %, respectively, in “early” and “late corpus luteum”. For ovarian tissue with hypofunction, compared to physiological conditions, a probable decrease in the content of the following isozymes was registered: GPO1 by 1.2–11.3 % (Р < 0.01), GPO4 – 6.8–19.7 % (Р < 0 .01) and GPO5 – 2.7–7.5 % (Р < 0.01), while the content of GPO3, on the contrary, increased by 23.6–29.2 % (Р < 0.05–0.01). The study of the dependence of the content of enzyme isozymes on different conditions of the gonad showed that the correlation ratio for the content of GPO isozymes in ovarian tissue under physiological conditions and hypofunction: medium strength was negative for GPO2 and GPO3 (η = 0.473 and 0.530), positive for GPO5 (η = 0.481 ), and strongly positive for GPO1 and GPO4 (η = 0.740 and 0.746).
{"title":"Isozymes of glutathione peroxidase in cow ovarian tissue under different physiological and pathological states of the gonad","authors":"M. М. Akymyshyn, N. Kuzmina, D. Ostapiv","doi":"10.32718/nvlvet11021","DOIUrl":"https://doi.org/10.32718/nvlvet11021","url":null,"abstract":"The article presents a study of glutathione peroxidase (GPO) isozymes content in ovarian tissue under various physiological and pathological states of cow gonads. The ovarian tissue of 12 cows in different physiological conditions were selected for research, from which homogenates were prepared. The content of isozymes was determined in the supernatant after centrifugation by electrophoresis. After electrophoresis in a 7.5 % polyacrylamide gel and specific staining of the plates according to Lin C. L. and Weydert C. J., 5 bands of catalytically active isozymes of the enzyme (GPO1, GPO2, GPO3, GPO4, and GPO5) were established. They differed in electrophoretic mobility, intensity, and area of band staining. It has been proven that depending on the physiological state of the ovaries, the content of individual isozymes in the gonad tissue changed. In particular, it was found that the content of GPO1 for “late corpus luteum” and “follicular growth” was almost the same, and was in the range of 14.2–15.6 %, and for “early corpus luteum” it was 8.7–10.1 % lower (Р < 0.01). The content of GPO2 was high in “early corpus luteum” (19.9 ± 6.42 %), and in “late corpus luteum” and “follicular growth” it was lower on 11.0–13.2 % (Р < 0.01). The content of GPO3 during “follicular growth” and “early corpus luteum” was within 50.1–51.2 %, and during “late” it decreased on 4.5–5.6 %. According to changes in the physiological state of the ovary in the tissue of “late corpus luteum”, the content of GPO4 was the highest (26.0 ± 2.40 %), and under “follicular growth” and “early corpus luteum” it was lower on 10.2 (Р < 0.05) and 12.9 % (Р < 0.01), respectively. A high content of GPO5 was established in the ovarian tissue of “follicular growth” (10.8 ± 2.00 %), which tended to decrease by 2.8 % and by 4.8 %, respectively, in “early” and “late corpus luteum”. For ovarian tissue with hypofunction, compared to physiological conditions, a probable decrease in the content of the following isozymes was registered: GPO1 by 1.2–11.3 % (Р < 0.01), GPO4 – 6.8–19.7 % (Р < 0 .01) and GPO5 – 2.7–7.5 % (Р < 0.01), while the content of GPO3, on the contrary, increased by 23.6–29.2 % (Р < 0.05–0.01). The study of the dependence of the content of enzyme isozymes on different conditions of the gonad showed that the correlation ratio for the content of GPO isozymes in ovarian tissue under physiological conditions and hypofunction: medium strength was negative for GPO2 and GPO3 (η = 0.473 and 0.530), positive for GPO5 (η = 0.481 ), and strongly positive for GPO1 and GPO4 (η = 0.740 and 0.746).","PeriodicalId":33662,"journal":{"name":"Naukovii visnik L''vivs''kogo natsional''nogo universitetu veterinarnoyi meditsini ta biotekhnologii imeni SZ G''zhits''kogo Seriia Kharchovi tekhnologiyi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42340960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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