Mesenchymal stem cells (MSCs) have been recognized as a therapeutic tool for various diseases due to its unique ability for tissue regeneration and immune regulation. However, poor survival during in vitro expansion and after being administrated in vivo limits its clinical uses. Accordingly, protocols for enhancing cell survivability is critical for establishing an efficient cell therapy is needed. CDDO-Me is a synthetic C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, which is known to stimulate nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway. Herein, report that CDDO-Me promoted the proliferation of MSCs and increased colony forming units (CFU) numbers. No alteration in differentiation into tri-lineage mesodermal cells was found after CDDO-Me treatment. We observed that CDDO-Me treatment reduced the cell death induced by oxidative stress, demonstrated by the augment in the expression of Nrf2-downstream genes. Lastly, CDDO-Me led to the nuclear translocation of NRF2. Our data indicate that CDDO-Me can enhance the functionality of MSCs by stimulating cell survival and increasing viability under oxidative stress.
{"title":"CDDO-Me alleviates oxidative stress in human mesenchymal stem cells","authors":"Hye Jin Cho, Tae Min Kim","doi":"10.12750/jarb.36.4.285","DOIUrl":"https://doi.org/10.12750/jarb.36.4.285","url":null,"abstract":"Mesenchymal stem cells (MSCs) have been recognized as a therapeutic tool for various diseases due to its unique ability for tissue regeneration and immune regulation. However, poor survival during in vitro expansion and after being administrated in vivo limits its clinical uses. Accordingly, protocols for enhancing cell survivability is critical for establishing an efficient cell therapy is needed. CDDO-Me is a synthetic C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, which is known to stimulate nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway. Herein, report that CDDO-Me promoted the proliferation of MSCs and increased colony forming units (CFU) numbers. No alteration in differentiation into tri-lineage mesodermal cells was found after CDDO-Me treatment. We observed that CDDO-Me treatment reduced the cell death induced by oxidative stress, demonstrated by the augment in the expression of Nrf2-downstream genes. Lastly, CDDO-Me led to the nuclear translocation of NRF2. Our data indicate that CDDO-Me can enhance the functionality of MSCs by stimulating cell survival and increasing viability under oxidative stress.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80661145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junyoung Kim, Youngwook Jung, H. Jung, Muhammad Shakeel, M. Yoon
Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcription-polymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes.
{"title":"Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes","authors":"Junyoung Kim, Youngwook Jung, H. Jung, Muhammad Shakeel, M. Yoon","doi":"10.12750/jarb.36.4.292","DOIUrl":"https://doi.org/10.12750/jarb.36.4.292","url":null,"abstract":"Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcription-polymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75701573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Traf4 is required for tight junction complex during mouse blastocyst formation","authors":"Jian Lee, I. Choi","doi":"10.12750/jarb.36.4.307","DOIUrl":"https://doi.org/10.12750/jarb.36.4.307","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78667495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
So-Yeon Jo, Yong Hwangbo, Sang-Hee Lee, H. Cheong, Dong-Ku Kim, Choon-keun Park
{"title":"Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics","authors":"So-Yeon Jo, Yong Hwangbo, Sang-Hee Lee, H. Cheong, Dong-Ku Kim, Choon-keun Park","doi":"10.12750/jarb.36.4.239","DOIUrl":"https://doi.org/10.12750/jarb.36.4.239","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88560370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doo Kim, Ji-Hwan Lee, G. Jang, Eun-Jeong Choi, Jin-Ju Kim, Jian Lee, J. Son
{"title":"The studies on characteristics of return to estrus in postpartum dairy cattle","authors":"Doo Kim, Ji-Hwan Lee, G. Jang, Eun-Jeong Choi, Jin-Ju Kim, Jian Lee, J. Son","doi":"10.12750/jarb.36.4.230","DOIUrl":"https://doi.org/10.12750/jarb.36.4.230","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90741335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assisted reproductive technologies (ARTs) have applications in a variety of fields including human clinics and basic science research (Mochida, 2020). In animals such as mice, ART is important for the conservation of genetic resources and the generation of genetically engineered animals (Hasegawa et al., 2017). Many ARTs have been developed, such as cryopreservation of sperm and embryos, superovulation, in vitro fertilization (IVF), embryo culture, and embryo transfer (ET) (Ha et al., 2013; Lee et al, 2015; Hasegawa et al., 2017; Lee et al., 2018; Barsuren et al., 2019; Chio et al., 2019; Kim and Park, 2019; Jung et al., 2020). The success of several ARTs such as cryopreservation or IVF and the production of genetically engineered mice is dependent upon the ability of successful pregnancy after ET, therefore ET is an indispensable related to ART in mice (Lamas et al., 2020). Many factors could affect successful pregnancy after ET, such as age or weight of the recipients, pseudopregnancy of recipients, the type of anesthetic and analgesic, genetic backgrounds of the embryo and recipient strains, embryo stage and quality, the number of transferred embryos, the genetic modifications of embryos, and the surgical technique (Bradford, 1979; Ertzeid and Storeng, 2001; Mahabir et al., 2018; Lamas et al., 2020). For conventional ET procedures, the preparation of pseudopregnant recipients is inefficient. Females at the Hormone induced recipients for embryo transfer in mice Yeonmi Lee and Eunju Kang* Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea Center for Embryo and Stem Cell Research, CHA Advanced Research Institute, CHA University, Seongnam 13488, Korea Original Article J Anim Reprod Biotechnol 2021;36:247-252 pISSN: 2671-4639 • eISSN: 2671-4663 https://doi.org/10.12750/JARB.36.4.247 JARB Journal of Animal Reproduction and Biotechnology
辅助生殖技术(ARTs)在包括人类诊所和基础科学研究在内的各个领域都有应用(Mochida, 2020)。在小鼠等动物中，ART对于遗传资源的保护和基因工程动物的产生非常重要(Hasegawa et al.， 2017)。许多人工授精技术已经被开发出来，例如精子和胚胎的低温保存、超排卵、体外受精(IVF)、胚胎培养和胚胎移植(ET) (Ha ET al.， 2013;Lee et al, 2015;Hasegawa et al.， 2017;Lee et al.， 2018;Barsuren等人，2019;Chio等人，2019;Kim and Park, 2019;Jung et al.， 2020)。低温保存或体外受精等几种ART的成功以及基因工程小鼠的产生取决于ET后成功妊娠的能力，因此ET是小鼠ART不可或缺的相关因素(Lamas ET al.， 2020)。许多因素可能影响体外受精后的成功妊娠，如受体的年龄或体重、受体的假妊娠、麻醉剂和镇痛药的类型、胚胎的遗传背景和受体品系、胚胎阶段和质量、移植胚胎的数量、胚胎的遗传修饰和手术技术(Bradford, 1979;Ertzeid and Storeng, 2001;Mahabir et al.， 2018;Lamas et al.， 2020)。对于传统的ET程序，假妊娠受体的准备是低效的。李延美，姜恩珠* CHA大学生命科学学院生物医学科学系，城南13488,CHA大学高级研究所韩国胚胎与干细胞研究中心，城南13488，韩国原创文章J动物生殖生物技术2021;36:247-252 pISSN: 2671-4639•eISSN: 2671-4663 https://doi.org/10.12750/JARB.36.4.247 JARB动物生殖与生物技术杂志
{"title":"Hormone induced recipients for embryo transfer in mice","authors":"Yeonmi Lee, Eunju Kang","doi":"10.12750/jarb.36.4.247","DOIUrl":"https://doi.org/10.12750/jarb.36.4.247","url":null,"abstract":"Assisted reproductive technologies (ARTs) have applications in a variety of fields including human clinics and basic science research (Mochida, 2020). In animals such as mice, ART is important for the conservation of genetic resources and the generation of genetically engineered animals (Hasegawa et al., 2017). Many ARTs have been developed, such as cryopreservation of sperm and embryos, superovulation, in vitro fertilization (IVF), embryo culture, and embryo transfer (ET) (Ha et al., 2013; Lee et al, 2015; Hasegawa et al., 2017; Lee et al., 2018; Barsuren et al., 2019; Chio et al., 2019; Kim and Park, 2019; Jung et al., 2020). The success of several ARTs such as cryopreservation or IVF and the production of genetically engineered mice is dependent upon the ability of successful pregnancy after ET, therefore ET is an indispensable related to ART in mice (Lamas et al., 2020). Many factors could affect successful pregnancy after ET, such as age or weight of the recipients, pseudopregnancy of recipients, the type of anesthetic and analgesic, genetic backgrounds of the embryo and recipient strains, embryo stage and quality, the number of transferred embryos, the genetic modifications of embryos, and the surgical technique (Bradford, 1979; Ertzeid and Storeng, 2001; Mahabir et al., 2018; Lamas et al., 2020). For conventional ET procedures, the preparation of pseudopregnant recipients is inefficient. Females at the Hormone induced recipients for embryo transfer in mice Yeonmi Lee and Eunju Kang* Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea Center for Embryo and Stem Cell Research, CHA Advanced Research Institute, CHA University, Seongnam 13488, Korea Original Article J Anim Reprod Biotechnol 2021;36:247-252 pISSN: 2671-4639 • eISSN: 2671-4663 https://doi.org/10.12750/JARB.36.4.247 JARB Journal of Animal Reproduction and Biotechnology","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86494186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yesong Kim, J. Yun, Seon Jeong Moon, J. Seong, H. Kong
A number of Korean Chicken breeds were registered in Domestic Animal Diversity Information System (DAD-IS, http://dad.fao.org/) of the Food and Agriculture Organization (FAO). Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. Therefore, this study aimed to analysis the genetic diversity and relationship of 22 Korean Chicken breeds using 12 microsatellite (MS) markers. The mean number of alleles for each variety was 5.52, ranging from a 3.75 (Leghorn F; NF) to a 7.0 (Ross). The most diverse breed was the Hanhyup3 (HCC), which had the highest expected heterozygosity (H Exp ) (0.754) and polymorphic information content (PIC) (0.711). The NF was the least diverse population, having the lowest H Exp (0.467) and PIC (0.413). As a result of the principal coordinates analysis (PCoA) and factorial correspondence analysis (FCA) confirmed that Hy-line Brown (HL) and Lohmann Brown (LO) are very close to each other and that Leghorn and Rhode Island Red (RIR) are clearly distinguished from other groups. Thus, the reliability and power of identification using 12 types of MS markers were improved, and the genetic diversity and probability of individual discrimination were confirmed through statistical analysis. This study is expected to be used as basic data for the identification of Korean chicken breeds, and our results indicated that these multiplex PCR marker sets will have considerable applications in population genetic structure analysis.
在联合国粮食及农业组织(FAO)的家畜多样性信息系统(DAD-IS, http://dad.fao.org/)中，有不少韩国鸡品种被登记。评价地方品种的遗传多样性和亲缘关系是鉴定独特和有价值遗传资源的重要因素。因此，本研究旨在利用12个微卫星(MS)标记分析22个韩国鸡品种的遗传多样性和亲缘关系。每个品种的平均等位基因数为5.52个，从3.75个(Leghorn F;NF)到7.0(罗斯)。品种多样性最高的是汉化3号(HCC)，其期望杂合度(H Exp)和多态性信息含量(PIC)分别为0.754和0.711。NF种群多样性最低，H Exp(0.467)和PIC(0.413)最低。主坐标分析(PCoA)和析因对应分析(FCA)证实了hyline Brown (HL)和Lohmann Brown (LO)的亲缘关系非常接近，Leghorn和Rhode Island Red (RIR)与其他类群有明显的区别。从而提高了12种MS标记的鉴定可靠性和鉴定能力，并通过统计分析确认了遗传多样性和个体歧视概率。本研究可作为鉴定韩国鸡品种的基础数据，结果表明多重PCR标记集在群体遗传结构分析中具有重要的应用价值。
{"title":"Genetic diversity and relationship of Korean chicken breeds using 12 microsatellite markers","authors":"Yesong Kim, J. Yun, Seon Jeong Moon, J. Seong, H. Kong","doi":"10.12750/jarb.36.3.154","DOIUrl":"https://doi.org/10.12750/jarb.36.3.154","url":null,"abstract":"A number of Korean Chicken breeds were registered in Domestic Animal Diversity Information System (DAD-IS, http://dad.fao.org/) of the Food and Agriculture Organization (FAO). Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. Therefore, this study aimed to analysis the genetic diversity and relationship of 22 Korean Chicken breeds using 12 microsatellite (MS) markers. The mean number of alleles for each variety was 5.52, ranging from a 3.75 (Leghorn F; NF) to a 7.0 (Ross). The most diverse breed was the Hanhyup3 (HCC), which had the highest expected heterozygosity (H Exp ) (0.754) and polymorphic information content (PIC) (0.711). The NF was the least diverse population, having the lowest H Exp (0.467) and PIC (0.413). As a result of the principal coordinates analysis (PCoA) and factorial correspondence analysis (FCA) confirmed that Hy-line Brown (HL) and Lohmann Brown (LO) are very close to each other and that Leghorn and Rhode Island Red (RIR) are clearly distinguished from other groups. Thus, the reliability and power of identification using 12 types of MS markers were improved, and the genetic diversity and probability of individual discrimination were confirmed through statistical analysis. This study is expected to be used as basic data for the identification of Korean chicken breeds, and our results indicated that these multiplex PCR marker sets will have considerable applications in population genetic structure analysis.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90267944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eun-Jin Kim, L. Dang, Marie Merci Nyiramana, A. Siregar, M. Woo, Chang-Woon Kim, D. Kang
The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicin-induced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.
{"title":"TRPV1 activation induces cell death of TM3 mouse Leydig cells","authors":"Eun-Jin Kim, L. Dang, Marie Merci Nyiramana, A. Siregar, M. Woo, Chang-Woon Kim, D. Kang","doi":"10.12750/jarb.36.3.145","DOIUrl":"https://doi.org/10.12750/jarb.36.3.145","url":null,"abstract":"The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicin-induced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74790350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Oh, J. Ha, J. Yi, Daehyun Kim, S. Oh, Songmi Kim, Kyudong Han, Yong-Soo Park
{"title":"Investigation of single nucleotide polymorphism in TSH-β and CaSR associated with body weight in Korean native chickens (Gray Brown)","authors":"D. Oh, J. Ha, J. Yi, Daehyun Kim, S. Oh, Songmi Kim, Kyudong Han, Yong-Soo Park","doi":"10.12750/jarb.36.3.129","DOIUrl":"https://doi.org/10.12750/jarb.36.3.129","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78404001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyeong-ju You, Ga-Yeon Kim, Seung-Yeon Kim, M. Kang
Both first authors contributed equally to this work. ABSTRACT Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl2. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 μm of CdCl2, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl2 untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl2 in HC11 cells decreased the mRNA expression of Rad51.
{"title":"DNA recombinase Rad51 is regulated with UVinduced DNA damage and the DNA mismatch repair inhibitor CdCl2 in HC11 cells","authors":"Hyeong-ju You, Ga-Yeon Kim, Seung-Yeon Kim, M. Kang","doi":"10.12750/jarb.36.3.121","DOIUrl":"https://doi.org/10.12750/jarb.36.3.121","url":null,"abstract":"Both first authors contributed equally to this work. ABSTRACT Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl2. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 μm of CdCl2, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl2 untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl2 in HC11 cells decreased the mRNA expression of Rad51.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"404 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77700103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：