S. Druzhinina, M. Loginova, D. N. Smirnova, I. P. Obukhov, K. O. Yaroshenko, E. Poponina, E. Nazarova, N. Isaeva, I. Paramonov
The use of mesenchymal stem cells (MSCs), which have a pronounced immunomodulatory activity, is a promising direction in the development of biomedical cell preparations (BMCPs). In oncohematology, the use of BMCPs containing MSCs is aimed at supporting hematopoiesis during cotransplantation with hematopoietic stem cells (HSCs) and suppressing immune conflicts during allogeneic unrelated transplantation and severe autoimmune processes. An obligatory stage of registration of BMCPs is confirmation of the identity of the MSC cell line (CL), which includes the establishment of morphological characteristics, evaluation of the expression of specific markers and proteins, and confirmation of the genetic stability of CL during cultivation. Determination of markers of genetic stability is possible using various methods, however, according to the recommendations of the American National Standardization Institute, the standard is the analysis of short tandem repeats (STR analysis). The purpose of the study is to develop an algorithm for determining the authenticity of BMCPs containing MSCs, including STR analysis. Material and methods. Identification of MSC cells in BMCP was performed according to the criteria of the International Society for Cell Therapy. Viable cells were counted in a Goryaev chamber. Immunophenotypic characteristics of MSCs were determined by flow cytometry. The level of production of specific proteins was assessed using enzyme immunoassay. Genetic stability markers were identified by STR analysis. Results and discussion. The methods were tested in triplicate for ten BMCP samples to confirm the reproducibility and reliability of the results. The developed algorithm for determining the authenticity of BMCP has a high accuracy, as it includes the STR analysis technique, which makes it possible to identify 19 polymorphic STR markers located on different alleles. Using the method will allow BMCP manufacturers to go through the procedure of state registration of drugs.
{"title":"Algorithm for determining the authenticity of biomedical cell preparations containing mesenchymal stem cells","authors":"S. Druzhinina, M. Loginova, D. N. Smirnova, I. P. Obukhov, K. O. Yaroshenko, E. Poponina, E. Nazarova, N. Isaeva, I. Paramonov","doi":"10.18699/ssmj20230109","DOIUrl":"https://doi.org/10.18699/ssmj20230109","url":null,"abstract":"The use of mesenchymal stem cells (MSCs), which have a pronounced immunomodulatory activity, is a promising direction in the development of biomedical cell preparations (BMCPs). In oncohematology, the use of BMCPs containing MSCs is aimed at supporting hematopoiesis during cotransplantation with hematopoietic stem cells (HSCs) and suppressing immune conflicts during allogeneic unrelated transplantation and severe autoimmune processes. An obligatory stage of registration of BMCPs is confirmation of the identity of the MSC cell line (CL), which includes the establishment of morphological characteristics, evaluation of the expression of specific markers and proteins, and confirmation of the genetic stability of CL during cultivation. Determination of markers of genetic stability is possible using various methods, however, according to the recommendations of the American National Standardization Institute, the standard is the analysis of short tandem repeats (STR analysis). The purpose of the study is to develop an algorithm for determining the authenticity of BMCPs containing MSCs, including STR analysis. Material and methods. Identification of MSC cells in BMCP was performed according to the criteria of the International Society for Cell Therapy. Viable cells were counted in a Goryaev chamber. Immunophenotypic characteristics of MSCs were determined by flow cytometry. The level of production of specific proteins was assessed using enzyme immunoassay. Genetic stability markers were identified by STR analysis. Results and discussion. The methods were tested in triplicate for ten BMCP samples to confirm the reproducibility and reliability of the results. The developed algorithm for determining the authenticity of BMCP has a high accuracy, as it includes the STR analysis technique, which makes it possible to identify 19 polymorphic STR markers located on different alleles. Using the method will allow BMCP manufacturers to go through the procedure of state registration of drugs.","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41868970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. A. Gaponov, E. G. Dmitrieva, Ya. S. Malov, A. Iakimov
Knowledge in interrelations between gross anatomy of the left atrial appendage (LAA) and thickness of the walls of LAA and periauricular area enables decreasing operational risks in LAA ostium occluding and «Cox-Maze» surgery for atrial fibrillation. The aim of the study was to identify significant interrelations between the macroscopic characteristics of the LAA (size, shape, number of lobes) and the parameters of the wall thickness of the LAA and its isthmus. Material and methods. The study includes 50 heart specimens of patients died from non-cardiac diseases. We examined 60 anatomical sections from 30 hearts by means Olympus SZX2-ZB10 microscope, and histological slices from 20 hearts. Results. The layers of the LAA wall were thinner than those in the isthmus. The thickness of LAA walls did not show direct correlation with the external dimensions of LAA. We found inverse correlation (Rs = – 0.4, p < 0,05) between the thickness of the myocardium, endocardium and some external sizes of LAA. The wall thickness of «chicken wing», «cauliflower» and «arrowhead» was the same. The wall of single-lobe LAA was thinner than that of two-lobed LAA (p = 0.036). The LAA isthmus wall was thinner (p = 0.03) in hearts with «cauliflower» LAA compared to hearts with LAA resembled a «chicken wing». Differences in wall thickness in LAA of various shapes were due to the degree of subepicardial fatty tissue development. Intracardiac operations should be done with the utmost care in patients with «cauliflower» LAA and single-lobe LAA to avoid damage of the LAA and periauricular area. Conclusions. The research found clinically significant interrelations between the LAA wall thickness and the number of its lobes as well as between the LAA isthmus wall thickness and LAA shape variants.
{"title":"Does the wall thickness of the left atrial appendage and its isthmus depend on their macroscopic characteristics?","authors":"A. A. Gaponov, E. G. Dmitrieva, Ya. S. Malov, A. Iakimov","doi":"10.18699/ssmj20230104","DOIUrl":"https://doi.org/10.18699/ssmj20230104","url":null,"abstract":"Knowledge in interrelations between gross anatomy of the left atrial appendage (LAA) and thickness of the walls of LAA and periauricular area enables decreasing operational risks in LAA ostium occluding and «Cox-Maze» surgery for atrial fibrillation. The aim of the study was to identify significant interrelations between the macroscopic characteristics of the LAA (size, shape, number of lobes) and the parameters of the wall thickness of the LAA and its isthmus. Material and methods. The study includes 50 heart specimens of patients died from non-cardiac diseases. We examined 60 anatomical sections from 30 hearts by means Olympus SZX2-ZB10 microscope, and histological slices from 20 hearts. Results. The layers of the LAA wall were thinner than those in the isthmus. The thickness of LAA walls did not show direct correlation with the external dimensions of LAA. We found inverse correlation (Rs = – 0.4, p < 0,05) between the thickness of the myocardium, endocardium and some external sizes of LAA. The wall thickness of «chicken wing», «cauliflower» and «arrowhead» was the same. The wall of single-lobe LAA was thinner than that of two-lobed LAA (p = 0.036). The LAA isthmus wall was thinner (p = 0.03) in hearts with «cauliflower» LAA compared to hearts with LAA resembled a «chicken wing». Differences in wall thickness in LAA of various shapes were due to the degree of subepicardial fatty tissue development. Intracardiac operations should be done with the utmost care in patients with «cauliflower» LAA and single-lobe LAA to avoid damage of the LAA and periauricular area. Conclusions. The research found clinically significant interrelations between the LAA wall thickness and the number of its lobes as well as between the LAA isthmus wall thickness and LAA shape variants.","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43155339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Pakhomova, E. L. Strokova, A. A. Korytkin, V. V. Kozhevnikov, A. F. Gusev, A. M. Zaydman
The neural crest has long attracted the attention of evolutionary biologists and, more recently, clinical specialists, as research in recent decades has significantly expanded the boundaries of knowledge about the involvement of neural crest and neural crest cells in the development of human pathology. The neural crest and neural crest cells are a unique evolutionarily based embryonic structure. Its discovery completely changed the view of the process of embryogenesis. Knowledge of neural crest development sheds light on many of the most «established» questions of developmental biology and evolution. Our article will reflect on the historical stages of the discovery and study of the neural crest and the impact of this discovery on entrenched ideas about germ layer specificity and the theory of germ layers – the reasoning of the neural crest as the fourth germ layer. The aim of this review is to describe the history of the discovery and study of neural crest and neural crest cells based on an analysis of the literature. In writing this article, an analysis of the scientific literature was conducted using the search terms «neural crest», «neural crest cells», «neural crest cell morphology», «germinal layers» and «embryonic development» in the computer databases PubMed, Scopus, Web of Science, and eLibrary. The depth of the analytical search corresponds to the period of the discovery of the neural crest and the first mention of the neural crest as an embryonic morphological structure in the scientific literature. The information presented confirms the high interest of research scientists and clinical specialists in the study of neural crest and neural crest cells. The involvement of neural crest cells in the formation of somatic and musculoskeletal pathologies has received particular attention in recent decades. The literature sources are represented by 169 full-text manuscripts and monographs mainly in English. Conclusions. Neural crest and neural crest cells are unique evolutionary structures. Regularities of formation, reasons which condition migration, differentiation, interaction of neural crest cells with other structures during embryogenesis as well as their potential, which is realized in postnatal period, continue to be the subject of research up to now.
神经嵴长期以来一直吸引着进化生物学家的注意，最近，临床专家的注意，因为近几十年来的研究已经显著地扩展了关于神经嵴和神经嵴细胞在人类病理发展中的参与的知识边界。神经嵴和神经嵴细胞是一种独特的基于进化的胚胎结构。它的发现彻底改变了人们对胚胎发生过程的看法。神经嵴发育的知识揭示了许多最“既定”的发育生物学和进化问题。我们的文章将反思神经嵴的发现和研究的历史阶段，以及这一发现对根深蒂固的关于生殖层特异性和生殖层理论的影响-神经嵴作为第四生殖层的推理。这篇综述的目的是在文献分析的基础上描述神经嵴和神经嵴细胞的发现和研究的历史。在撰写本文时，使用检索词“神经嵴”、“神经嵴细胞”、“神经嵴细胞形态学”、“生发层”和“胚胎发育”在计算机数据库PubMed、Scopus、Web of Science和library中进行了科学文献分析。分析研究的深度与神经嵴的发现和科学文献中首次提到神经嵴作为胚胎形态结构的时期相对应。所提供的信息证实了研究科学家和临床专家对神经嵴和神经嵴细胞研究的高度兴趣。近几十年来，神经嵴细胞参与躯体和肌肉骨骼病变的形成受到了特别的关注。文献来源以169篇以英文为主的全文手稿和专著为代表。结论。神经嵴和神经嵴细胞是一种独特的进化结构。神经嵴细胞在胚胎发育过程中的形成规律、迁移、分化的原因、与其他结构的相互作用及其在出生后实现的潜能，至今仍是研究的课题。
{"title":"History of the study of the neural crest (review)","authors":"N. Pakhomova, E. L. Strokova, A. A. Korytkin, V. V. Kozhevnikov, A. F. Gusev, A. M. Zaydman","doi":"10.18699/ssmj20230102","DOIUrl":"https://doi.org/10.18699/ssmj20230102","url":null,"abstract":"The neural crest has long attracted the attention of evolutionary biologists and, more recently, clinical specialists, as research in recent decades has significantly expanded the boundaries of knowledge about the involvement of neural crest and neural crest cells in the development of human pathology. The neural crest and neural crest cells are a unique evolutionarily based embryonic structure. Its discovery completely changed the view of the process of embryogenesis. Knowledge of neural crest development sheds light on many of the most «established» questions of developmental biology and evolution. Our article will reflect on the historical stages of the discovery and study of the neural crest and the impact of this discovery on entrenched ideas about germ layer specificity and the theory of germ layers – the reasoning of the neural crest as the fourth germ layer. The aim of this review is to describe the history of the discovery and study of neural crest and neural crest cells based on an analysis of the literature. In writing this article, an analysis of the scientific literature was conducted using the search terms «neural crest», «neural crest cells», «neural crest cell morphology», «germinal layers» and «embryonic development» in the computer databases PubMed, Scopus, Web of Science, and eLibrary. The depth of the analytical search corresponds to the period of the discovery of the neural crest and the first mention of the neural crest as an embryonic morphological structure in the scientific literature. The information presented confirms the high interest of research scientists and clinical specialists in the study of neural crest and neural crest cells. The involvement of neural crest cells in the formation of somatic and musculoskeletal pathologies has received particular attention in recent decades. The literature sources are represented by 169 full-text manuscripts and monographs mainly in English. Conclusions. Neural crest and neural crest cells are unique evolutionary structures. Regularities of formation, reasons which condition migration, differentiation, interaction of neural crest cells with other structures during embryogenesis as well as their potential, which is realized in postnatal period, continue to be the subject of research up to now.","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44919541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to establish the age and sex patterns of the topography of the ascending and descending colons of children and adolescents according to intravital imaging data. Material and methods. Computed tomograms of the abdomen of 88 children and adolescents without visible abdominal organs pathology were analyzed. The surveyedwere divided into 4 age groups: periods of early, first and second childhood, adolescence. The study was performed on 16-slice computed tomographs with the determination of the distances from the ascending and descending colon to the surface of the body, neighboring organs and anatomical structures of the abdomen, skeletotopia. The data obtained were subjected to variation-statistical processing with the determination of the median, the values of the 25th and 75th percentiles and the significance of differences according to the Mann – Whitney U test. Results and discussion. The article presents the topographic and anatomical characteristics of the ascending and descending colons of children and adolescents and establishes the patterns of change, taking into account the sex and age of the examined. Conclusions. A significant increase in the distance from the ascending colon to the right ureter, jejunum and ileum, and quadratus lumborum was determined. The distance from the body surface to the ascending colon increased with age along all the studied lines. The proximal part of the ascending colon among all those examined was mostly defined from the intervertebral disc LIV–LV to the intervertebral disc LV–SI, and the hepatic flexure of the colon was defined at the level from LI to the intervertebral disc LII–LIII. A significant increase in the distance from the descending colon to the left ureter, pancreas and quadratus lumborum was found. The distance from the body surface to the descending colon increased with age along the anterior median, left middle axillary, and left scapular lines. The splenic flexure of the colon was located at the level from LI to the intervertebral disc LI–LII, and the distal part of the descending colon was located at the level from LIV to the intervertebral disc LIV–LV.
{"title":"Topographic anatomy of the ascending and descending colons of children and adolescents according to intravital imaging","authors":"A. S. Lozinskiy, S. V. Chemezov","doi":"10.18699/ssmj20230111","DOIUrl":"https://doi.org/10.18699/ssmj20230111","url":null,"abstract":"The aim of the study was to establish the age and sex patterns of the topography of the ascending and descending colons of children and adolescents according to intravital imaging data. Material and methods. Computed tomograms of the abdomen of 88 children and adolescents without visible abdominal organs pathology were analyzed. The surveyedwere divided into 4 age groups: periods of early, first and second childhood, adolescence. The study was performed on 16-slice computed tomographs with the determination of the distances from the ascending and descending colon to the surface of the body, neighboring organs and anatomical structures of the abdomen, skeletotopia. The data obtained were subjected to variation-statistical processing with the determination of the median, the values of the 25th and 75th percentiles and the significance of differences according to the Mann – Whitney U test. Results and discussion. The article presents the topographic and anatomical characteristics of the ascending and descending colons of children and adolescents and establishes the patterns of change, taking into account the sex and age of the examined. Conclusions. A significant increase in the distance from the ascending colon to the right ureter, jejunum and ileum, and quadratus lumborum was determined. The distance from the body surface to the ascending colon increased with age along all the studied lines. The proximal part of the ascending colon among all those examined was mostly defined from the intervertebral disc LIV–LV to the intervertebral disc LV–SI, and the hepatic flexure of the colon was defined at the level from LI to the intervertebral disc LII–LIII. A significant increase in the distance from the descending colon to the left ureter, pancreas and quadratus lumborum was found. The distance from the body surface to the descending colon increased with age along the anterior median, left middle axillary, and left scapular lines. The splenic flexure of the colon was located at the level from LI to the intervertebral disc LI–LII, and the distal part of the descending colon was located at the level from LIV to the intervertebral disc LIV–LV.","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49264167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Shnyakin, P. G. Rudenko, A. Botov, I. A. Kazadaeva
The article presents an overview of modern scientific publications on echinococcosis and alveococcosis of the brain, which occur in 1–4 % of cases among all volumetric formations of the central nervous system. Despite the fact that these parasitic diseases are more common in endemic areas of developing countries in Asia, South America, Australia and New Zealand, isolated clinical cases are observed everywhere, including due to population migration, and they must bedifferentiated, first of all, from intracerebral cysts, abscesses, cystic tumors. Clinical manifestations of echinococcosis and alveococcosis of the brain include the development of hypertensive symptoms, focal neurological deficit, convulsive syndrome (with cortical localization of cysts). The article presents the modern possibilities of diagnostic methods (among which the main role is played by neuroimaging methods, such as multislice computed tomography and magnetic resonance imaging using contrast/paramagnet), and surgical and medical treatment of patients with echinococcosis and alveococcosis of the brain. The article also describes two own clinical observations of patients who were hospitalized in the neurosurgical department of the Regional Clinical Hospital (Krasnoyarsk).
{"title":"Echinococcosis and alveococcosis of the brain in the practice of a neurosurgeon (a review literature and clinical cases)","authors":"P. Shnyakin, P. G. Rudenko, A. Botov, I. A. Kazadaeva","doi":"10.18699/ssmj20230103","DOIUrl":"https://doi.org/10.18699/ssmj20230103","url":null,"abstract":"The article presents an overview of modern scientific publications on echinococcosis and alveococcosis of the brain, which occur in 1–4 % of cases among all volumetric formations of the central nervous system. Despite the fact that these parasitic diseases are more common in endemic areas of developing countries in Asia, South America, Australia and New Zealand, isolated clinical cases are observed everywhere, including due to population migration, and they must bedifferentiated, first of all, from intracerebral cysts, abscesses, cystic tumors. Clinical manifestations of echinococcosis and alveococcosis of the brain include the development of hypertensive symptoms, focal neurological deficit, convulsive syndrome (with cortical localization of cysts). The article presents the modern possibilities of diagnostic methods (among which the main role is played by neuroimaging methods, such as multislice computed tomography and magnetic resonance imaging using contrast/paramagnet), and surgical and medical treatment of patients with echinococcosis and alveococcosis of the brain. The article also describes two own clinical observations of patients who were hospitalized in the neurosurgical department of the Regional Clinical Hospital (Krasnoyarsk).","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41948200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taking into account the conflicting literature data and the ambiguity in the interpretation of parameters of diastolic heart function in patients with coronary heart disease, we conducted an analysis of diastolic heart function in patients after myocardial infarction at the outpatient rehabilitation stage. Aim of the study was to investigate the diagnostic information content and the feasibility of diastolic stress testing to assess the functional condition of postinfarction myocardium. Material and methods. 86 patients were examined at the outpatient stage of rehabilitation 6 weeks after myocardial infarction with coronary artery stenting ad hoc. The control group consisted of 10 healthy volunteers. Structural and functional examination of the heart was performed at rest and immediately after exercise using stress echocardiography. Results and discussion. Patients after myocardial infarction have a larger indexed volume of the left atrium (30.71 ± 8.88 vs. 20.49 ± 4.04 ml/m2) and an E/e` ratio (8.45 ± 3.27 vs. 6.46 ± 1.42) in comparison with the control group. 38 (62.3 %) patients with normal left ventricular (LV) ejection fraction (EF) had unimpaired LV diastolic function, 19 (31.1 %) and 2 (3.3 %) patients had type 1 and type 2 diastolic dysfunction, respectively, 2 patients (3,3 %) had an indeterminate result. Patients with reduced LV EF have a significantly lower early diastolic mitral annular velocity (e`). The diastolic stress test revealed a significant post-exercise increase in E/e` in only one patient (from 8.92 to 18.37), who also had an initially reduced EF (32 %). The stress test showed no significant changes in diastolic heart parameters after exercise in patients suffering from heart failure with preserved EF, which may indicate relatively good diastolic reserves of the heart. Conclusions. Myocardial infarction is accompanied by the presence of LV diastolic dysfunction in 53,5 % of the patients at the 6th week of the rehabilitation period. The diastolic stress test is accompanied by a rare occurrence of stress-induced diastolic dysfunction (4 %) in patients with reduced LV EF after myocardial infarction.
{"title":"Diastolic stress test in assessing functional state of postinfarction myocardium","authors":"A. M. Vorobev, V. Ruzov","doi":"10.18699/ssmj20230110","DOIUrl":"https://doi.org/10.18699/ssmj20230110","url":null,"abstract":"Taking into account the conflicting literature data and the ambiguity in the interpretation of parameters of diastolic heart function in patients with coronary heart disease, we conducted an analysis of diastolic heart function in patients after myocardial infarction at the outpatient rehabilitation stage. Aim of the study was to investigate the diagnostic information content and the feasibility of diastolic stress testing to assess the functional condition of postinfarction myocardium. Material and methods. 86 patients were examined at the outpatient stage of rehabilitation 6 weeks after myocardial infarction with coronary artery stenting ad hoc. The control group consisted of 10 healthy volunteers. Structural and functional examination of the heart was performed at rest and immediately after exercise using stress echocardiography. Results and discussion. Patients after myocardial infarction have a larger indexed volume of the left atrium (30.71 ± 8.88 vs. 20.49 ± 4.04 ml/m2) and an E/e` ratio (8.45 ± 3.27 vs. 6.46 ± 1.42) in comparison with the control group. 38 (62.3 %) patients with normal left ventricular (LV) ejection fraction (EF) had unimpaired LV diastolic function, 19 (31.1 %) and 2 (3.3 %) patients had type 1 and type 2 diastolic dysfunction, respectively, 2 patients (3,3 %) had an indeterminate result. Patients with reduced LV EF have a significantly lower early diastolic mitral annular velocity (e`). The diastolic stress test revealed a significant post-exercise increase in E/e` in only one patient (from 8.92 to 18.37), who also had an initially reduced EF (32 %). The stress test showed no significant changes in diastolic heart parameters after exercise in patients suffering from heart failure with preserved EF, which may indicate relatively good diastolic reserves of the heart. Conclusions. Myocardial infarction is accompanied by the presence of LV diastolic dysfunction in 53,5 % of the patients at the 6th week of the rehabilitation period. The diastolic stress test is accompanied by a rare occurrence of stress-induced diastolic dysfunction (4 %) in patients with reduced LV EF after myocardial infarction.","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41465827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Type 1 diabetes mellitus (DM) is one of the most prevalent endocrinological disorders of childhood. This disorder is accompanied by numerous micro- and macrovascular complications that reduce children’s life expectancy and increase healthcare costs. Central nervous system (CNS) damage in children and adolescents with type 1 DM is a fairly common but poorly studied complication. The leading role among CNS changes is played by cognitive dysfunction (CD). The pathophysiological aspects of CD in type 1 DM haven’t yet been understood completely. The three main triggers are hypoglycemia, chronic moderate hyperglycemia, and acute hyperglycemia (diabetic ketoacidosis). Evidence is presented to show the development of morphological abnormalities of the brain with progression of type 1 DM in children. Damage in the formation of white and gray matter, hippocampus, amygdala, the processes of myelination disorders have been described, which can also be the cause of CD development in this contingent of patients. Peculiarities ofclinical manifestations of CD in children and adolescents with type 1 DM depending on the localization of the brain lesion are described. A more pronounced CD was found in cases when DM debuts in early childhood (up to 5 years). Timely diagnosis and correction of CD in children and adolescents with type 1 DM can improve the therapeutic and prophylactic prognosis in this disease.
{"title":"Cognitive impairments in type 1 diabetes mellitus","authors":"Y. Bykov, V. Baturin","doi":"10.18699/ssmj20230101","DOIUrl":"https://doi.org/10.18699/ssmj20230101","url":null,"abstract":"Type 1 diabetes mellitus (DM) is one of the most prevalent endocrinological disorders of childhood. This disorder is accompanied by numerous micro- and macrovascular complications that reduce children’s life expectancy and increase healthcare costs. Central nervous system (CNS) damage in children and adolescents with type 1 DM is a fairly common but poorly studied complication. The leading role among CNS changes is played by cognitive dysfunction (CD). The pathophysiological aspects of CD in type 1 DM haven’t yet been understood completely. The three main triggers are hypoglycemia, chronic moderate hyperglycemia, and acute hyperglycemia (diabetic ketoacidosis). Evidence is presented to show the development of morphological abnormalities of the brain with progression of type 1 DM in children. Damage in the formation of white and gray matter, hippocampus, amygdala, the processes of myelination disorders have been described, which can also be the cause of CD development in this contingent of patients. Peculiarities ofclinical manifestations of CD in children and adolescents with type 1 DM depending on the localization of the brain lesion are described. A more pronounced CD was found in cases when DM debuts in early childhood (up to 5 years). Timely diagnosis and correction of CD in children and adolescents with type 1 DM can improve the therapeutic and prophylactic prognosis in this disease.","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41987482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. V. Verkhoturova, V. Gorbunov, S. Tsarenok, T. Aksenova, N. A. Iljamakova
{"title":"Serum bone resorption markers and 25-hydroxyvitamin D level in women of the Trans-Baikal region of the Russian and Buryat nationalities","authors":"S. V. Verkhoturova, V. Gorbunov, S. Tsarenok, T. Aksenova, N. A. Iljamakova","doi":"10.18699/ssmj20220612","DOIUrl":"https://doi.org/10.18699/ssmj20220612","url":null,"abstract":"","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44620424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. V. Raikova, N. Komleva, A. Starshov, S. Mazilov, T. A. Novikova
{"title":"Risk factors for the development of bronchopulmonary pathology in workers producing synthetic polyacrylonitrile fiber","authors":"S. V. Raikova, N. Komleva, A. Starshov, S. Mazilov, T. A. Novikova","doi":"10.18699/ssmj20220611","DOIUrl":"https://doi.org/10.18699/ssmj20220611","url":null,"abstract":"","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41705497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. V. Pavlova, L. Syromyatnikova, S. Mekhryakov, S. A. Chazov, A. Kulesh
{"title":"Paradoxical embolism with the development of ischemic stroke on the background of pulmonary embolism: comparative analysis of two clinical cases","authors":"A. V. Pavlova, L. Syromyatnikova, S. Mekhryakov, S. A. Chazov, A. Kulesh","doi":"10.18699/ssmj20220610","DOIUrl":"https://doi.org/10.18699/ssmj20220610","url":null,"abstract":"","PeriodicalId":33781,"journal":{"name":"Sibirskii nauchnyi meditsinskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46952018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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