Onion (Allium cepa) is a valuable vegetable and a candidate for sustainable waste management in agri-food industry. The purpose of the current paper was to research the utilization of onion peels to an economical substrate for inulinase production by Yarrowia lipolytica ISF7 strain under solid state fermentation (SSF). SSF is preferred to obtain an effective and low-cost inulinase production. The medium designation was optimized using Taguchi design of experiment. For this purpose, Taguchi L9 orthogonal array layout was applied using the moisture content, initial pH and incubation time as the selected factors at three levels. The results showed that the minimum inulinase activity 22.7 U g-1 of dry substrate (ds) was determined using the 6th experimental setup while the highest inulinase activity 292.2 U gds-1 was measured from 5th experimental setup. The predicted value was determined as 311.6 U gds-1 which was closer to the obtained result (305.1 U gds-1). Consequently, an effective inulinase production can be achieved by Y. lipolytica ISF7 using onion peels as an economic substrate under SSF.
洋葱(Allium cepa)是一种有价值的蔬菜，也是农业食品工业中可持续废物管理的候选者。本论文的目的是研究利用洋葱皮作为溶脂耶氏菌ISF7固态发酵生产菊粉酶的经济底物。SSF是获得高效、低成本菊粉酶生产的首选方法。采用田口试验设计对培养基进行优化。为此，采用田口L9正交阵列布局，以水分含量、初始pH和孵育时间为3个水平的选择因素。结果表明，第6个试验装置测定的菊粉酶活性最低为22.7 U g-1，第5个试验装置测定的菊粉酶活性最高为292.2 U g-1。预测值为311.6 U gds-1，与实际结果(305.1 U gds-1)较为接近。因此，在SSF下，以洋葱皮作为经济底物的聚脂Y. ISF7可以有效地生产菊粉酶。
{"title":"Use of onion peels as an economical substrate for microbial inulinase production under solid state fermentation","authors":"Özden CANLI TAŞAR, G. Tasar","doi":"10.46239/ejbcs.1163946","DOIUrl":"https://doi.org/10.46239/ejbcs.1163946","url":null,"abstract":"Onion (Allium cepa) is a valuable vegetable and a candidate for sustainable waste management in agri-food industry. The purpose of the current paper was to research the utilization of onion peels to an economical substrate for inulinase production by Yarrowia lipolytica ISF7 strain under solid state fermentation (SSF). SSF is preferred to obtain an effective and low-cost inulinase production. The medium designation was optimized using Taguchi design of experiment. For this purpose, Taguchi L9 orthogonal array layout was applied using the moisture content, initial pH and incubation time as the selected factors at three levels. The results showed that the minimum inulinase activity 22.7 U g-1 of dry substrate (ds) was determined using the 6th experimental setup while the highest inulinase activity 292.2 U gds-1 was measured from 5th experimental setup. The predicted value was determined as 311.6 U gds-1 which was closer to the obtained result (305.1 U gds-1). Consequently, an effective inulinase production can be achieved by Y. lipolytica ISF7 using onion peels as an economic substrate under SSF.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115953924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the study, in addition to the antibacterial effect of astaxanthin on Aeromonas hydrophila and A. sobria strains, the presence of virulence genes (Aero, act, ast, and hylA) and antibiotic resistance genes (tetC and sulI) in the strains was investigated. Antibiotic profiles of the strains were also investigated as part of the study. Strains were identified by conventional biochemical tests and PCR assay using a 16S rDNA primer pair specific for A. hydrophila. According to the results of bacteriological and molecular studies, two of the six Aeromonas strains were identified as A. hydrophila and four of them as A. sobria. The Aero virulence gene and the act virulence gene were found in all strains, while the ast and hylA virulence genes were detected only in A. hydrophila strains. All strains were resistant to chloramphenicol, tetracycline, nalidixic acid, and ampicillin in the standard disk diffusion test. Although all strains showed resistance to tetracycline and moderate resistance to oxytetracycline in the antibiogram tests, tetC antibiotic resistance gene was not detected in the strains and sulI antibiotic resistance gene was not detected in the strains. In the study, acetone solutions containing 0.1 g and 0.5 g of astaxanthin were found to have an antibacterial effect on A. hydrophila strains. Acetone solutions containing 0.1 g, 0.5 g, and 1.0 g of astaxanthin showed antibacterial effects on A. sobria strains. It was found that 0.1 g, 0.5 g, and 1.0 g astaxanthin solutions prepared with methanol and distilled water had no antibacterial effects on the strains.
{"title":"Investigation of the Antibacterial Effect of Astaxanthin and the Prevalence of Virulence and Antimicrobial Resistance Genes of Aeromonas spp.","authors":"J. Korun, Aycan Ulutaş","doi":"10.46239/ejbcs.1150114","DOIUrl":"https://doi.org/10.46239/ejbcs.1150114","url":null,"abstract":"In the study, in addition to the antibacterial effect of astaxanthin on Aeromonas hydrophila and A. sobria strains, the presence of virulence genes (Aero, act, ast, and hylA) and antibiotic resistance genes (tetC and sulI) in the strains was investigated. Antibiotic profiles of the strains were also investigated as part of the study. Strains were identified by conventional biochemical tests and PCR assay using a 16S rDNA primer pair specific for A. hydrophila. According to the results of bacteriological and molecular studies, two of the six Aeromonas strains were identified as A. hydrophila and four of them as A. sobria. The Aero virulence gene and the act virulence gene were found in all strains, while the ast and hylA virulence genes were detected only in A. hydrophila strains. All strains were resistant to chloramphenicol, tetracycline, nalidixic acid, and ampicillin in the standard disk diffusion test. Although all strains showed resistance to tetracycline and moderate resistance to oxytetracycline in the antibiogram tests, tetC antibiotic resistance gene was not detected in the strains and sulI antibiotic resistance gene was not detected in the strains. In the study, acetone solutions containing 0.1 g and 0.5 g of astaxanthin were found to have an antibacterial effect on A. hydrophila strains. Acetone solutions containing 0.1 g, 0.5 g, and 1.0 g of astaxanthin showed antibacterial effects on A. sobria strains. It was found that 0.1 g, 0.5 g, and 1.0 g astaxanthin solutions prepared with methanol and distilled water had no antibacterial effects on the strains.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128455740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried out to determine the major phospholipids in selected dairy products (Beta Serum, Procream and Phospholipids-Rich Dairy products), evaluate the accuracy of the developed fat extraction method for liquid dairy samples and to compare the major phospholipids of different dairy samples obtained using the HPLC-UVvis and 31P-NMR methods. It was found that the developed fat extraction method can be used to estimate the lipid content of liquid dairy samples were still a bit satisfactory. Using the HPLC-UVvis method, it was found that the sphingomyelin (SM) consists of 2 curves in Beta Serum, Procream and Phospholipid-Rich products. The phosphatidylethanolamine (PE) separated ahead of phosphatidylcholine (PC) and then PC ahead of SM1 and SM2. The results showed that the data of the major phospholipids (PC, PE and SM) in Butter Serum, Procream and Phospholipids-Rich products as determined by the HPLC-UVvis method compared well with those of the 31P-NMR method It must be noted that the analysis temperature for the HPLC-UVvis method was at 40oC while the 31P-NMR method was at 30oC, and this might have also contributed to the slight variation of the results. Furthermore, the HPLC-UVvis method is rapid and with cheaper analysis cost compared with the 31P-NMR method.
{"title":"Major Phospholipids of Selected Dairy Products as Determined by the HPLC-UVvis and 31P-NMR Methods","authors":"L. Diamante","doi":"10.46239/ejbcs.1141216","DOIUrl":"https://doi.org/10.46239/ejbcs.1141216","url":null,"abstract":"This study was carried out to determine the major phospholipids in selected dairy products (Beta Serum, Procream and Phospholipids-Rich Dairy products), evaluate the accuracy of the developed fat extraction method for liquid dairy samples and to compare the major phospholipids of different dairy samples obtained using the HPLC-UVvis and 31P-NMR methods. It was found that the developed fat extraction method can be used to estimate the lipid content of liquid dairy samples were still a bit satisfactory. Using the HPLC-UVvis method, it was found that the sphingomyelin (SM) consists of 2 curves in Beta Serum, Procream and Phospholipid-Rich products. The phosphatidylethanolamine (PE) separated ahead of phosphatidylcholine (PC) and then PC ahead of SM1 and SM2. The results showed that the data of the major phospholipids (PC, PE and SM) in Butter Serum, Procream and Phospholipids-Rich products as determined by the HPLC-UVvis method compared well with those of the 31P-NMR method It must be noted that the analysis temperature for the HPLC-UVvis method was at 40oC while the 31P-NMR method was at 30oC, and this might have also contributed to the slight variation of the results. Furthermore, the HPLC-UVvis method is rapid and with cheaper analysis cost compared with the 31P-NMR method.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129889902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried out to determine the palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA) or anandamide contents in selected fish wastes, treating the fish wastes with highest PEA and AEA with different concentration of monoethanolamine (MEA) solution, incubation temperature and time, as well as the ratio of MEA solution to fish waste to further increase its PEA and AEA contents. Based on the results of the preliminary experiment, a fractional factorial design experiments was done with 4 factors including MEA concentration, incubation time, incubation temperature and dosing ratio (MEA solution:salmon guts). The results showed that the MEA content ranged from 2.25 to 8.06 mg/g sample, the PEA content ranged from 17.4 to 300.2 µg/g sample while the AEA content ranged from 1.3 to 19.0 µg/g sample all on a wet weight basis of all the FD treated samples. The FD treated sample with the highest MEA, PEA and AEA using an MEA solution concentration of 250mM from pure MEA chemical, incubation time of 0.5 hour, incubation temperature of 6oC and a dose ratio of 6 mL MEA solution:100 g salmon guts. The MEA, PEA and AEA contents of the different samples were analysed using the Yates algorithm to determine which of the four factors were more important. The results showed that MEA, PEA and AEA contents were significantly affected by the concentration of MEA solution used in dosing the salmon guts, followed by the incubation time and then a slight effect of dosing ratio while the incubation temperature has no significant effect.
{"title":"Monoethanolamine Treatment of Fish Wastes and Salmon Guts to Increase It Palmitoylethanolamide and Anandamide Contents","authors":"L. Diamante","doi":"10.46239/ejbcs.1141865","DOIUrl":"https://doi.org/10.46239/ejbcs.1141865","url":null,"abstract":"This study was carried out to determine the palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA) or anandamide contents in selected fish wastes, treating the fish wastes with highest PEA and AEA with different concentration of monoethanolamine (MEA) solution, incubation temperature and time, as well as the ratio of MEA solution to fish waste to further increase its PEA and AEA contents. \u0000Based on the results of the preliminary experiment, a fractional factorial design experiments was done with 4 factors including MEA concentration, incubation time, incubation temperature and dosing ratio (MEA solution:salmon guts). The results showed that the MEA content ranged from 2.25 to 8.06 mg/g sample, the PEA content ranged from 17.4 to 300.2 µg/g sample while the AEA content ranged from 1.3 to 19.0 µg/g sample all on a wet weight basis of all the FD treated samples. The FD treated sample with the highest MEA, PEA and AEA using an MEA solution concentration of 250mM from pure MEA chemical, incubation time of 0.5 hour, incubation temperature of 6oC and a dose ratio of 6 mL MEA solution:100 g salmon guts. The MEA, PEA and AEA contents of the different samples were analysed using the Yates algorithm to determine which of the four factors were more important. The results showed that MEA, PEA and AEA contents were significantly affected by the concentration of MEA solution used in dosing the salmon guts, followed by the incubation time and then a slight effect of dosing ratio while the incubation temperature has no significant effect.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132951699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Binnaz Kirbiyik, B. Mazmancı, Şeyma Gülnaz Yarlılar, Naz Ugur, K. Ocakoğlu
Drug delivery systems have been used in cancer treatment to increase drug effectiveness. The hydroxyapatite (HAP) based materials used in this area can provide drug transport to the target site without its deterioration. In this study, porous hollow hydroxyapatite microspheres (PHHMs) were produced by using the hydrothermal method. Tamoxifen (TAM) used in the treatment of breast cancer has been covalently attached to the produced microspheres. The obtained microsphere structures (tamoxifen-loaded hydroxyapatite, TAM/H) were successfully characterized by ATR-FTIR, FE-SEM, XRD, and DLS methods. The breast cancer cell line MCF-7 was used to examine the effect of the hybrid structure. The cytotoxic and genotoxic effects of TAM/H was compared with the TAM groups on MCF-7. Our results have showed that, the decrease in cell viability at 24 and 36 hours were still continued at 48 hours only in TAM/H groups. In addition, TAM/H was found to show a genotoxic affect by the increment in genetic damage index (GDI) and damaged cell percentage (DCP%). As a result, use of hydroxyapatite was suitable for the transport of TAM and that covalent binding was suitable for drug particle interaction with hybrid structure and thus controlled drug release occurred.
{"title":"Tamoxifen Delivery to Breast Cancer Cells (MCF-7) Via Hydroxyapatite Microspheres","authors":"Binnaz Kirbiyik, B. Mazmancı, Şeyma Gülnaz Yarlılar, Naz Ugur, K. Ocakoğlu","doi":"10.46239/ejbcs.1040161","DOIUrl":"https://doi.org/10.46239/ejbcs.1040161","url":null,"abstract":"Drug delivery systems have been used in cancer treatment to increase drug effectiveness. The hydroxyapatite (HAP) based materials used in this area can provide drug transport to the target site without its deterioration. In this study, porous hollow hydroxyapatite microspheres (PHHMs) were produced by using the hydrothermal method. Tamoxifen (TAM) used in the treatment of breast cancer has been covalently attached to the produced microspheres. The obtained microsphere structures (tamoxifen-loaded hydroxyapatite, TAM/H) were successfully characterized by ATR-FTIR, FE-SEM, XRD, and DLS methods. The breast cancer cell line MCF-7 was used to examine the effect of the hybrid structure. The cytotoxic and genotoxic effects of TAM/H was compared with the TAM groups on MCF-7. Our results have showed that, the decrease in cell viability at 24 and 36 hours were still continued at 48 hours only in TAM/H groups. In addition, TAM/H was found to show a genotoxic affect by the increment in genetic damage index (GDI) and damaged cell percentage (DCP%). As a result, use of hydroxyapatite was suitable for the transport of TAM and that covalent binding was suitable for drug particle interaction with hybrid structure and thus controlled drug release occurred.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"613 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116454758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Güneş ışığının etkisini azaltan özel boyalarda kullanılan serin pigmentler genellikle perilenler yardımıyla üretilir. Beyaz boyalar güneş ışığını geniş bir yelpazede yansıtırken, siyah ve diğer koyu renkler geniş bir spektrumu absorbe ettikleri için uygulandıkları malzemelerin ısınmasına etki ederler. “Serin pigmentler” (boyalar) olarak adlandırılan moleküller, koyu renkli olmalarına rağmen güneş ışınlarının NIR bölgesinde çok düşük absorpsiyon göstermeleri nedeniyle ilgi görmektedir. Bu çalışmada, 12 farklı perilen bazlı pigment kuantum hesaplamaları ile analiz edilmiş, yansımaları ve özellikleri arasındaki korelasyonlar incelenmiştir. Hesaplamalarda Hartree-Fock hesaplama yöntemi ve 3-21G temel seti kullanılarak Gaussian 9 Revision D.01 de yapılmıştır. Yardımcı ara yüzey yazılımı olarak Gaussview 5.0.8 kullanılmıştır. Sentezlenen perilenlerin karakterizasyonu Ft-IR, NMR, XRD ile yapılmış ve daha önceki çalışmalarda yayınlanmıştır. Simülasyondan elde edilen entalpi, HOMO.LUMO aralığı, simetri ve dipol momentleri karşılaştırılmış ve sonuç olarak NIR yansımasının pigmentlerin dipol momentleri ile ilişkili olduğu tespit edilmiştir.
{"title":"Perilen bazlı serin pigmentlerin moleküler dinamik simulasyonu ve NIR bölgesinin yansımasında yapısal özelliklerin incelenmesi","authors":"Güray Kilinççeker, Farhad Zari̇fi̇","doi":"10.46239/ejbcs.1139843","DOIUrl":"https://doi.org/10.46239/ejbcs.1139843","url":null,"abstract":"Güneş ışığının etkisini azaltan özel boyalarda kullanılan serin pigmentler genellikle perilenler yardımıyla üretilir. Beyaz boyalar güneş ışığını geniş bir yelpazede yansıtırken, siyah ve diğer koyu renkler geniş bir spektrumu absorbe ettikleri için uygulandıkları malzemelerin ısınmasına etki ederler. “Serin pigmentler” (boyalar) olarak adlandırılan moleküller, koyu renkli olmalarına rağmen güneş ışınlarının NIR bölgesinde çok düşük absorpsiyon göstermeleri nedeniyle ilgi görmektedir. Bu çalışmada, 12 farklı perilen bazlı pigment kuantum hesaplamaları ile analiz edilmiş, yansımaları ve özellikleri arasındaki korelasyonlar incelenmiştir. Hesaplamalarda Hartree-Fock hesaplama yöntemi ve 3-21G temel seti kullanılarak Gaussian 9 Revision D.01 de yapılmıştır. Yardımcı ara yüzey yazılımı olarak Gaussview 5.0.8 kullanılmıştır. Sentezlenen perilenlerin karakterizasyonu Ft-IR, NMR, XRD ile yapılmış ve daha önceki çalışmalarda yayınlanmıştır. Simülasyondan elde edilen entalpi, HOMO.LUMO aralığı, simetri ve dipol momentleri karşılaştırılmış ve sonuç olarak NIR yansımasının pigmentlerin dipol momentleri ile ilişkili olduğu tespit edilmiştir.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122923853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Investigation various nitrogen sources effects on the production of invertase by Aspergillus niger was researched in this study. Invertase is a precious enzyme used in many industries like food, pharmacy, confectionery, invert syrup production. Taguchi design of experiment (DOE) was preferred to optimize the cultivation conditions. L16 (43) orthogonal array was selected in the current study including nitrogen source, initial pH of the medium and incubation time at four levels for statistical optimization. The data showed that optimized version of invertase production was achieved using proteose peptone, 5.5 initial pH and 3 days for incubation time. Bacto peptone had higher enzyme activity than casein and yeast extract. pH of the medium was found as the most efficient factor among nitrogen source and incubation time. Besides, percentage contribution of the nitrogen source and incubation time were indicated at similar rates (9 and 10%, respectively). The highest enzyme activity was defined as 45.87 U/ml, which was found to be closer to the predicted result (46.33 U/ml). As a conclusion, proteose peptone increased the invertase activity and use of Taguchi DOE supported quick and effective optimization.
{"title":"Effects of different nitrogen sources on invertase production by Aspergillus niger","authors":"Özden CANLI TAŞAR, G. Tasar","doi":"10.46239/ejbcs.1138487","DOIUrl":"https://doi.org/10.46239/ejbcs.1138487","url":null,"abstract":"Investigation various nitrogen sources effects on the production of invertase by Aspergillus niger was researched in this study. Invertase is a precious enzyme used in many industries like food, pharmacy, confectionery, invert syrup production. Taguchi design of experiment (DOE) was preferred to optimize the cultivation conditions. L16 (43) orthogonal array was selected in the current study including nitrogen source, initial pH of the medium and incubation time at four levels for statistical optimization. The data showed that optimized version of invertase production was achieved using proteose peptone, 5.5 initial pH and 3 days for incubation time. Bacto peptone had higher enzyme activity than casein and yeast extract. pH of the medium was found as the most efficient factor among nitrogen source and incubation time. Besides, percentage contribution of the nitrogen source and incubation time were indicated at similar rates (9 and 10%, respectively). The highest enzyme activity was defined as 45.87 U/ml, which was found to be closer to the predicted result (46.33 U/ml). As a conclusion, proteose peptone increased the invertase activity and use of Taguchi DOE supported quick and effective optimization.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131077179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, it was aimed to compare the antioxidant properties of the extracts obtained from the extraction of olive leaves in Hatay province prepared with different solvents. For this purpose, olive leaves were extracted using pure methyl alcohol with soxhlet and also by maceration using 60% ethanol, 70% methanol, 90% acetone (v/v) and distilled water. Total Flavonoid Content (TFC), Total Phenolic Content (TPC), Ferric Antioxidant Reducing Power (FRAP), DPPH radical scavenging avctivity, ABTS Cation Radical Scavenging activity and total sulfydryl groups by Ellman methods were used to determine the antioxidant properties of the extracts. As a result of the study, the highest TFC values per g leaf and g extract were obtained for 70% methanol maceration and soxhlet-methanol extract, respectively, while the highest TPC observed per leaf and g extract were determined in the extracts obtained with 90% acetone. In the FRAP method, the extract obtained with 60% Ethanol showed the highest activity per g leaf and g extract. Extracts obtained with Soxhlet showed the highest activity for both ABTS activity and Ellman method. In the DPPH method, the lowest EC50 value was determined in the extract obtained using 70% methanol, and it was determined that the extracts obtained with water showed the lowest performance in all antioxidant activity methods.
{"title":"Investigation of antioxidant properties of olive leave extracts from Hatay by different extraction methods","authors":"Seda Ağçam, G. Ozyilmaz","doi":"10.46239/ejbcs.1122284","DOIUrl":"https://doi.org/10.46239/ejbcs.1122284","url":null,"abstract":"In this study, it was aimed to compare the antioxidant properties of the extracts obtained from the extraction of olive leaves in Hatay province prepared with different solvents. For this purpose, olive leaves were extracted using pure methyl alcohol with soxhlet and also by maceration using 60% ethanol, 70% methanol, 90% acetone (v/v) and distilled water. Total Flavonoid Content (TFC), Total Phenolic Content (TPC), Ferric Antioxidant Reducing Power (FRAP), DPPH radical scavenging avctivity, ABTS Cation Radical Scavenging activity and total sulfydryl groups by Ellman methods were used to determine the antioxidant properties of the extracts. As a result of the study, the highest TFC values per g leaf and g extract were obtained for 70% methanol maceration and soxhlet-methanol extract, respectively, while the highest TPC observed per leaf and g extract were determined in the extracts obtained with 90% acetone. In the FRAP method, the extract obtained with 60% Ethanol showed the highest activity per g leaf and g extract. Extracts obtained with Soxhlet showed the highest activity for both ABTS activity and Ellman method. In the DPPH method, the lowest EC50 value was determined in the extract obtained using 70% methanol, and it was determined that the extracts obtained with water showed the lowest performance in all antioxidant activity methods.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115772376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adana ili Saimbeyli ilçesi ve Osmaniye ili Yarpuz Yaylası Bezelik Daz’ında 2021 yılı Nisan-Eylül ayları arasında yapılan arazi gözlem çalışmalarında Işgın Zümrütü-Minik Zümrüt (Callophrys mystaphia) tespit edilmiştir. Tür, Adana ve Osmaniye illeri için yeni kayıt niteliğindedir. Türün erkek bireyinin morfolojik tarifi ve bazı parametreleri ölçülerek çalışmamızda verilmiştir.
{"title":"Türkiye'nin Adana ve Osmaniye illerinde Callophrys mystaphia Miller, 1913 (Lepidoptera: Lycaenidae: Eumaeini)’nın İlk Kaydı","authors":"Erol Atay, Zeynel Cebeci, Mehmet Çelik","doi":"10.46239/ejbcs.1125683","DOIUrl":"https://doi.org/10.46239/ejbcs.1125683","url":null,"abstract":"Adana ili Saimbeyli ilçesi ve Osmaniye ili Yarpuz Yaylası Bezelik Daz’ında 2021 yılı Nisan-Eylül ayları arasında yapılan arazi gözlem çalışmalarında Işgın Zümrütü-Minik Zümrüt (Callophrys mystaphia) tespit edilmiştir. Tür, Adana ve Osmaniye illeri için yeni kayıt niteliğindedir. Türün erkek bireyinin morfolojik tarifi ve bazı parametreleri ölçülerek çalışmamızda verilmiştir.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126520109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Azırak, D. Tastemir Korkmaz, S. Bilgiç, M. Özgöçmen, M. Özer
Valproic acid (VA), widely used as an antiepileptic, causes structural and functional kidney disorders. Whether thymoquinone (TQ) has a beneficial effect on valproic acid (VA)-induced nephrotoxicity has been investigated. Twenty-one male Spraque Dawley rats were grouped into control, VA, and VA + TQ groups (n=7 for per group). VA (500 mg/kg/day) and TQ (50 mg/kg/day) were applied to the rats orally for 14 days. They were euthanized on the 15th day of the treatment. The cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) gene expression levels, biochemical parameters, total antioxidant/oxidant statuses (TAS/TOS), oxidative stress index (OSI), histological and immunohistochemical analysis were performed to evaluate kidney toxicity. In the VA + TQ group, COX-1 expression levels increased, while COX-2 expression levels decreased. While the creatinine (Cr) and blood urea nitrogen (BUN) levels, production of caspase-3 (CAS-3) and NADPH oxidase-4 (NOX-4) were increased in the VA-treated group, they were decreased in VA + TQ group. Treatment with TQ against VA administration decreased TOS and OSI levels while increasing TAS. TQ protects the kidney against the toxic effects of VA.
{"title":"Thymoquinone Prevents Valproic Acid-Induced Nephrotoxicity in Rat Kidney","authors":"S. Azırak, D. Tastemir Korkmaz, S. Bilgiç, M. Özgöçmen, M. Özer","doi":"10.46239/ejbcs.1123892","DOIUrl":"https://doi.org/10.46239/ejbcs.1123892","url":null,"abstract":"Valproic acid (VA), widely used as an antiepileptic, causes structural and functional kidney disorders. Whether thymoquinone (TQ) has a beneficial effect on valproic acid (VA)-induced nephrotoxicity has been investigated. Twenty-one male Spraque Dawley rats were grouped into control, VA, and VA + TQ groups (n=7 for per group). VA (500 mg/kg/day) and TQ (50 mg/kg/day) were applied to the rats orally for 14 days. They were euthanized on the 15th day of the treatment. The cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) gene expression levels, biochemical parameters, total antioxidant/oxidant statuses (TAS/TOS), oxidative stress index (OSI), histological and immunohistochemical analysis were performed to evaluate kidney toxicity. In the VA + TQ group, COX-1 expression levels increased, while COX-2 expression levels decreased. While the creatinine (Cr) and blood urea nitrogen (BUN) levels, production of caspase-3 (CAS-3) and NADPH oxidase-4 (NOX-4) were increased in the VA-treated group, they were decreased in VA + TQ group. Treatment with TQ against VA administration decreased TOS and OSI levels while increasing TAS. TQ protects the kidney against the toxic effects of VA.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130627311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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