In this study, the in vitro antidiabetic, antioxidant activity and total flavonoid content (TFC) and total phenolic content (TPC) of vinegar obtained from endemic Crataegus tanacetifolia (Lam.) Pers. (Rosaceae), (hawthorn) were examined. Kemal Unlu brand hawthorn vinegar obtained from Malatya province (MS) and Temmuz Organic Farm vinegar obtained from Konya were used as study material. Their antidiabetic activity was determined by α-amylase and α-glucosidase inhibitory methods. Antioxidant activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferrous iron chelating (FCA) assays. The absorbance were read in the Elisa reader and evaluated with Excel and GraphPad programs. The MS has been found to have higher α- amylase (95.12± 3.71%) and α-glucosidase inhibitory (81.62 ± 0.33%) effects. The TS demonstrated (94.13 ± 3.85%) α-amylase and (75.35 ± 2.19%) α-glucosidase inhibitory activity, respectively. The TPC was found to be in TS (467.59 ± 6.73) mg GAE/mL MS (328.46 ± 5.50) mg GAE/mL. The TFC was found as (1.94 ± 10.36) mg CE/mL and (1.32 ± 10.96) mg CE/mL in TS and MS vinegar, respectively. The FCA was found to be in TS (33.37 ± 0.53%) MS (31.08 ± 10.87%). The DPPH radical scavenging activity was found as (73.82 ± 2.12%) in TS and (80.12 ± 4.45%) in MS. ABTS radical scavenging activity was found to be the highest in TS with (82.51± 0.78%) and in MS found as (78.65 ± 0.55%). The antidiabetic, antioxidant activity, TPC and TFC determinations of these vinegars were performed for the first time with these methods.
{"title":"Investigation of in vitro antidiabetic and antioxidant activity of hawthorn vinegar obtained from Endemic Crataegus tanacetifolia (Poir.) Pers.","authors":"Feyza Akgün, Nigar Sıla Tuğlu, Yasemin Gülbahar Açil, Nuraniye Eruygur","doi":"10.46239/ejbcs.1228402","DOIUrl":"https://doi.org/10.46239/ejbcs.1228402","url":null,"abstract":"In this study, the in vitro antidiabetic, antioxidant activity and total flavonoid content (TFC) and total phenolic content (TPC) of vinegar obtained from endemic Crataegus tanacetifolia (Lam.) Pers. (Rosaceae), (hawthorn) were examined. Kemal Unlu brand hawthorn vinegar obtained from Malatya province (MS) and Temmuz Organic Farm vinegar obtained from Konya were used as study material. Their antidiabetic activity was determined by α-amylase and α-glucosidase inhibitory methods. Antioxidant activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferrous iron chelating (FCA) assays. The absorbance were read in the Elisa reader and evaluated with Excel and GraphPad programs. The MS has been found to have higher α- amylase (95.12± 3.71%) and α-glucosidase inhibitory (81.62 ± 0.33%) effects. The TS demonstrated (94.13 ± 3.85%) α-amylase and (75.35 ± 2.19%) α-glucosidase inhibitory activity, respectively. The TPC was found to be in TS (467.59 ± 6.73) mg GAE/mL MS (328.46 ± 5.50) mg GAE/mL. The TFC was found as (1.94 ± 10.36) mg CE/mL and (1.32 ± 10.96) mg CE/mL in TS and MS vinegar, respectively. The FCA was found to be in TS (33.37 ± 0.53%) MS (31.08 ± 10.87%). The DPPH radical scavenging activity was found as (73.82 ± 2.12%) in TS and (80.12 ± 4.45%) in MS. ABTS radical scavenging activity was found to be the highest in TS with (82.51± 0.78%) and in MS found as (78.65 ± 0.55%). The antidiabetic, antioxidant activity, TPC and TFC determinations of these vinegars were performed for the first time with these methods.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"1 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sentetik boyar maddeler tekstil, kozmetik, gıda, mobilya, ilaç ve otomotiv endüstrisi gibi yaygın kullanım alanları nedeniyle ticari öneme sahip endüstriyel ürünlerdir. Birçok ülkede kullanılan bu boyaların yaklaşık 10.000 çeşidi bulunmaktadır. Yıllık 700.000 ton üretim hacmine sahip olan boyar maddeler, tüm endüstriyel atık miktarının önemli bir kısmını (1/5) oluşturmaktadırlar. Tekstil endüstrisine ait deşarjların tarım alanlarına ve su kaynaklarına karışması toprak gözeneklerinin tıkanıp verimin düşmesine, içme ve sulama suyunun insan tüketimi için elverişsiz hale gelmesine neden olmaktadır. Bu çalışmada üç farklı tekstil boyasının (Süperfix Black NNX (SBNNX), Syanacryl Black XFDL (SBXFDL) ve Reaktive Blue 19 (RB19) mutajenik ve rekombinojenik etkileri in vivo olarak Drosophila kanat benek testi ile belirlenmiştir.Bu amaçla distile su negatif ve EMS pozitif kontrol grupları hazırlanmıştır. Ayrıca uygulama grupları için de farklı dozlarda her üç tekstil boyası (150, 300 ve 450 ppm) kullanılmıştır. Elde edilen verilere göre, tüm tekstil boyaları hem mutajenik hem de rekombinojenik etkili bulunmuştur. Uygulama gruplarına ait veriler, distile su kontrol grubu ile karşılaştırıldığında aralarındaki fark istatistiki olarak anlamlıdır (p SBXFDL > RB19 olarak belirlenmiştir.
合成染料是具有重要商业价值的工业产品，广泛应用于纺织、化妆品、食品、家具、制药和汽车等行业。许多国家使用的合成染料约有 10 000 种。染料的年产量为 700.000 吨，占所有工业废物的很大一部分（1/5）。纺织工业向农业区和水资源的排放会导致土壤孔隙堵塞，土壤肥力下降，不利于人类饮用和灌溉。本研究通过果蝇翅斑试验测定了三种不同纺织染料（Superfix Black NNX（SBNNX）、Syanacryl Black XFDL（SBXFDL）和 Reactivated Blue 19（RB19））在体内的致突变和重组效应。此外，处理组还使用了三种纺织品染料的不同剂量（150、300 和 450 ppm）。结果表明，所有纺织品染料都具有致突变性和致重组性。将处理组的数据与蒸馏水对照组的数据进行比较，两者之间的差异具有统计学意义（p SBXFDL > RB19.
{"title":"Endüstriyel amaçlı kullanılan bazı boyar maddelerin mutajenik etkilerinin Drosophila kanat benek testi ile in vitro olarak belirlenmesi","authors":"Handan Uysal, Hatice Çeli̇k","doi":"10.46239/ejbcs.1377815","DOIUrl":"https://doi.org/10.46239/ejbcs.1377815","url":null,"abstract":"Sentetik boyar maddeler tekstil, kozmetik, gıda, mobilya, ilaç ve otomotiv endüstrisi gibi yaygın kullanım alanları nedeniyle ticari öneme sahip endüstriyel ürünlerdir. Birçok ülkede kullanılan bu boyaların yaklaşık 10.000 çeşidi bulunmaktadır. Yıllık 700.000 ton üretim hacmine sahip olan boyar maddeler, tüm endüstriyel atık miktarının önemli bir kısmını (1/5) oluşturmaktadırlar. Tekstil endüstrisine ait deşarjların tarım alanlarına ve su kaynaklarına karışması toprak gözeneklerinin tıkanıp verimin düşmesine, içme ve sulama suyunun insan tüketimi için elverişsiz hale gelmesine neden olmaktadır. Bu çalışmada üç farklı tekstil boyasının (Süperfix Black NNX (SBNNX), Syanacryl Black XFDL (SBXFDL) ve Reaktive Blue 19 (RB19) mutajenik ve rekombinojenik etkileri in vivo olarak Drosophila kanat benek testi ile belirlenmiştir.Bu amaçla distile su negatif ve EMS pozitif kontrol grupları hazırlanmıştır. Ayrıca uygulama grupları için de farklı dozlarda her üç tekstil boyası (150, 300 ve 450 ppm) kullanılmıştır. Elde edilen verilere göre, tüm tekstil boyaları hem mutajenik hem de rekombinojenik etkili bulunmuştur. Uygulama gruplarına ait veriler, distile su kontrol grubu ile karşılaştırıldığında aralarındaki fark istatistiki olarak anlamlıdır (p SBXFDL > RB19 olarak belirlenmiştir.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the venom of the species Leiurus abdullahbayrami, known as the yellow scorpion from the Buthidae family, was applied for the first time as a natural corrosion inhibitor for iron in hydrochloric acid solution. The effectiveness of scorpion venom as an eco-friendly and natural inhibitor was determined by electrochemical methods such as electrochemical impedance spectroscopy (EIS), potentiodynamic polarization (Tafel extrapolation method) and linear polarization resistance (LPR) after an hour of immersion. Four different concentrations were determined for the green and natural inhibitor scorpion venom in 1.0 M HCl, and it was observed that the corrosion of iron in these solutions was significantly inhibited. In general, the inhibition efficiency was above 80%. According to the potentiodynamic polarization data, it has been determined that the Leiurus abdullahbayrami venom acts as a cathodic-type inhibitor on the Fe surface. Finally, the surface images of the iron electrodes in 1.0 M HCl solutions without and with Leiurus abdullahbayrami venom after 1 h immersion were examined by field emission scanning electron microscope (FE-SEM), it was concluded that the surface containing scorpion venom had a flatter compared to the uninhibited surface.
在这项研究中，首次将 Buthidae 科黄蝎 Leiurus abdullahbayrami 的毒液用作盐酸溶液中铁的天然缓蚀剂。浸泡一小时后，通过电化学阻抗光谱（EIS）、电位极化（Tafel 外推法）和线性极化电阻（LPR）等电化学方法测定了蝎毒作为环保型天然缓蚀剂的有效性。在 1.0 M HCl 溶液中测定了四种不同浓度的绿色天然抑制剂蝎毒，观察到这些溶液对铁的腐蚀有明显的抑制作用。一般来说，抑制效率在 80% 以上。根据电位极化数据，可以确定雷蛇毒在铁表面起着阴极型抑制剂的作用。最后，用场发射扫描电子显微镜（FE-SEM）观察了铁电极在 1.0 M HCl 溶液中浸泡 1 小时后的表面图像（不含蝎毒和含蝎毒），结果表明含蝎毒的表面比未受抑制的表面更平整。
{"title":"Application of a new inhibitor for the corrosion of iron in acidic solution: Electrochemical effect of a scorpion venom","authors":"D. Özkır, O. Seyyar","doi":"10.46239/ejbcs.1373150","DOIUrl":"https://doi.org/10.46239/ejbcs.1373150","url":null,"abstract":"In this study, the venom of the species Leiurus abdullahbayrami, known as the yellow scorpion from the Buthidae family, was applied for the first time as a natural corrosion inhibitor for iron in hydrochloric acid solution. The effectiveness of scorpion venom as an eco-friendly and natural inhibitor was determined by electrochemical methods such as electrochemical impedance spectroscopy (EIS), potentiodynamic polarization (Tafel extrapolation method) and linear polarization resistance (LPR) after an hour of immersion. Four different concentrations were determined for the green and natural inhibitor scorpion venom in 1.0 M HCl, and it was observed that the corrosion of iron in these solutions was significantly inhibited. In general, the inhibition efficiency was above 80%. According to the potentiodynamic polarization data, it has been determined that the Leiurus abdullahbayrami venom acts as a cathodic-type inhibitor on the Fe surface. Finally, the surface images of the iron electrodes in 1.0 M HCl solutions without and with Leiurus abdullahbayrami venom after 1 h immersion were examined by field emission scanning electron microscope (FE-SEM), it was concluded that the surface containing scorpion venom had a flatter compared to the uninhibited surface.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Free diving is a popular sport because of many features such as sustainability, eco-friendly and challenges to nature. Due to increased interest in recent years, the number of competitions is also increasing gradually. On the other hand, scientific reports on the understanding of longer breath-holding mechanisms and metabolisms are still unclear. To provide contributions on this phenomenon, glutathione peroxidase was selected as a model enzyme because of its critical importance in breath-holding. The enzymes from both human and free diving animals were compared by using bioinformatics tools such as ProtParam, Swiss-Model, Clustal Omega and the results are discussed in the present paper. In conclusion, the specific amino acid sequences can be considered in the selection of elite free divers for international competitions to get the best results. However, it should be noted that special training methods should also be applied to have better breath-holding capacities.
{"title":"Comparison of glutathione peroxidase-1 in free divers with their counterparts: A model study for sports informatics","authors":"Levent Çavaş, Elif Ci̇reli̇, Osman Ateş","doi":"10.46239/ejbcs.1251033","DOIUrl":"https://doi.org/10.46239/ejbcs.1251033","url":null,"abstract":"Free diving is a popular sport because of many features such as sustainability, eco-friendly and challenges to nature. Due to increased interest in recent years, the number of competitions is also increasing gradually. On the other hand, scientific reports on the understanding of longer breath-holding mechanisms and metabolisms are still unclear. To provide contributions on this phenomenon, glutathione peroxidase was selected as a model enzyme because of its critical importance in breath-holding. The enzymes from both human and free diving animals were compared by using bioinformatics tools such as ProtParam, Swiss-Model, Clustal Omega and the results are discussed in the present paper. In conclusion, the specific amino acid sequences can be considered in the selection of elite free divers for international competitions to get the best results. However, it should be noted that special training methods should also be applied to have better breath-holding capacities.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139317164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The development of anti-malarial drugs is of great importance due to the detrimental effects of this disease all around the world. In recent years, bioinformatics tools provide considerable contributions to develop new small molecules which have important bioactivities against many bio-targets. However, biases in the methodologies or aims of the studies in which in silico tools are used may reveal problematic cases. Hoslundal, hoslundin, and hoslunddiol were proposed by Shadrack et al. (2016) to inhibit Plasmodium falciparum lactate dehydrogenase (Pf-LDH) to fight malaria. But these molecules may have potential to inhibit mammalian LDHs. To investigate whether these molecules have inhibitions on mammalian LDHs or not, we studied a comprehensive and comparative molecular docking studies as described in the present paper. According to the results, the vina scores of hoslundal without NADH for Pf-LDH, HM-LDH, HH-LDH were found as -7.5, -7.6 and -8.2 kJ/mol, respectively. Moreover, multiple sequence alignment analysis reveals high similarities among sequences. In the light of molecular studies, hoslundal were found to be connected to Pf-LDH, HM-LDH, HH-LDH (31, 26, 34), (2, -7, 154), (11, 41, 54), respectively. In conclusion, novel small molecules which are developed via in silico tools could show excellent activities against bio-targets of the pathogenic microorganisms. However, it should not be forgotten that active site of the enzymes is conserved, therefore, after a possible proposal of small molecule, its molecular docking and also Swiss-ADME studies should be necessarily carried out.
{"title":"Possible inhibitory effects of hoslundal, hoslundin and hoslunddiol on human lactate dehydrogenases: a bioinformatics proof","authors":"Yagmur Bi̇lgi̇n, Yasir Yalnizoğlu, Levent Çavaş","doi":"10.46239/ejbcs.1281018","DOIUrl":"https://doi.org/10.46239/ejbcs.1281018","url":null,"abstract":"The development of anti-malarial drugs is of great importance due to the detrimental effects of this disease all around the world. In recent years, bioinformatics tools provide considerable contributions to develop new small molecules which have important bioactivities against many bio-targets. However, biases in the methodologies or aims of the studies in which in silico tools are used may reveal problematic cases. Hoslundal, hoslundin, and hoslunddiol were proposed by Shadrack et al. (2016) to inhibit Plasmodium falciparum lactate dehydrogenase (Pf-LDH) to fight malaria. But these molecules may have potential to inhibit mammalian LDHs. To investigate whether these molecules have inhibitions on mammalian LDHs or not, we studied a comprehensive and comparative molecular docking studies as described in the present paper. According to the results, the vina scores of hoslundal without NADH for Pf-LDH, HM-LDH, HH-LDH were found as -7.5, -7.6 and -8.2 kJ/mol, respectively. Moreover, multiple sequence alignment analysis reveals high similarities among sequences. In the light of molecular studies, hoslundal were found to be connected to Pf-LDH, HM-LDH, HH-LDH (31, 26, 34), (2, -7, 154), (11, 41, 54), respectively. In conclusion, novel small molecules which are developed via in silico tools could show excellent activities against bio-targets of the pathogenic microorganisms. However, it should not be forgotten that active site of the enzymes is conserved, therefore, after a possible proposal of small molecule, its molecular docking and also Swiss-ADME studies should be necessarily carried out.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the peroxidase-like activity of macroporous carbon manufactured using a silica template was investigated. The nanozyme activity of macroporous carbon was compared to commercial graphene oxide. The field emission scanning electron microscopy image of carbon revealed macroporous morphology. The nanozyme activity was studied via the catalytic oxidation of chromogenic substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) in the presence of hydrogen peroxide and the oxidized form of ABTS with a green color can be visualized by the eyes. Without functionalization and enzyme utilization, the fabricated macroporous carbon demonstrated green color development, indicating its peroxidase activity probably due to the large surface area and, thus, abundant active sites present on the surface. The oxygen-containing functional groups formed during carbonization act as active sites and can play a pivotal role in the peroxidase-mimicking activity.
{"title":"Makro gözenekli karbonun peroksidaz mimik aktivitesi","authors":"Bekir Çakiroğlu","doi":"10.46239/ejbcs.1215182","DOIUrl":"https://doi.org/10.46239/ejbcs.1215182","url":null,"abstract":"In this study, the peroxidase-like activity of macroporous carbon manufactured using a silica template was investigated. The nanozyme activity of macroporous carbon was compared to commercial graphene oxide. The field emission scanning electron microscopy image of carbon revealed macroporous morphology. The nanozyme activity was studied via the catalytic oxidation of chromogenic substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) in the presence of hydrogen peroxide and the oxidized form of ABTS with a green color can be visualized by the eyes. Without functionalization and enzyme utilization, the fabricated macroporous carbon demonstrated green color development, indicating its peroxidase activity probably due to the large surface area and, thus, abundant active sites present on the surface. The oxygen-containing functional groups formed during carbonization act as active sites and can play a pivotal role in the peroxidase-mimicking activity.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139347593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article is based on Geometridae species collected from Ardahan, Bayburt, Erzincan, Erzurum, Gumushane and Kars provinces in 2021. Geometridae species were collected using insect net and light traps. All collected samples were prepared and diagnosed in the laboratory. As a result, 4 subfamilies, 23 genera and 27 species were determined. Photos of all diagnosed species and material examined were presented. In addition, 7 from Ardahan, 8 from Bayburt, 5 from Erzincan, 5 from Erzurum, 8 from Gümüşhane and 3 from Kars were determined for the first time in this study Geometridae species.
{"title":"Contributions to the fauna of Geometridae in the north eastern provinces","authors":"M. Koyuncu, Murat Kütük, Mehmet Yaran","doi":"10.46239/ejbcs.1291057","DOIUrl":"https://doi.org/10.46239/ejbcs.1291057","url":null,"abstract":"This article is based on Geometridae species collected from Ardahan, Bayburt, Erzincan, Erzurum, Gumushane and Kars provinces in 2021. Geometridae species were collected using insect net and light traps. All collected samples were prepared and diagnosed in the laboratory. As a result, 4 subfamilies, 23 genera and 27 species were determined. Photos of all diagnosed species and material examined were presented. In addition, 7 from Ardahan, 8 from Bayburt, 5 from Erzincan, 5 from Erzurum, 8 from Gümüşhane and 3 from Kars were determined for the first time in this study Geometridae species.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139350690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışmada Hatay ili Kırıkhan-Reyhanlı bölgesi tarım topraklarının molibden içeriğinin belirlenmesi ve toprak içerisindeki bazı ağır metaller ile ilişkilerinin saptanması amaçlanmıştır. Bu amaç için Kırıkhan-Reyhanlı bölgesi tarım topraklarını temsil edecek şekilde iki farklı derinlik (0-20 ve 20-40 cm) ve 30 ayrı noktadan olmak üzere toplamda 60 toprak örneği alınmıştır. Alınan toprak örneklerinde; Kadmiyum (Cd), Cobalt (Co), Krom (Cr), Nikel (Ni), Bakır (Cu), Demir (Fe) ve Molibden (Mo) içerikleri belirlenmiştir. Araştırma sonuçlarına göre; toprakların Cd içerikleri 0.009-0.041 µg/kg; Co içeriği 0.011-0.317 µg/kg; Cr 0.008-0.187 µg/kg, Ni içerikleri 0.787-6.211 ppm; Cu içerikleri 1.11-3.77 ppm; Fe içerikleri 2.80-15.09 ve Mo içerikleri 0.006-0.101 µg/kg arasında bulunmuştur. Toprakların Mo ile Co ve Ni içerikleri arasında pozitif önemli ilişkiler belirlenirken Cr içeriği ile negatif önemli ilişkiler belirlenmiştir. Aynı zamanda Cd ile Co ve Ni; Co ile Ni ve Cu ile Fe aralarında ise pozitif önemli ilişkiler belirlenmiştir. Bölge topraklarının ağır metal içerikleri sınır değerler ile karşılaştırıldığında herhangi bir ağır metal kirliliğine rastlanmamıştır.
{"title":"Kırıkhan–Reyhanlı Bölgesi Tarım Topraklarının Molibden İçeriği ve Topraktaki Bazı Ağır Metaller ile İlişkilerinin Belirlenmesi","authors":"M. Yalçin","doi":"10.46239/ejbcs.1320487","DOIUrl":"https://doi.org/10.46239/ejbcs.1320487","url":null,"abstract":"Bu çalışmada Hatay ili Kırıkhan-Reyhanlı bölgesi tarım topraklarının molibden içeriğinin belirlenmesi ve toprak içerisindeki bazı ağır metaller ile ilişkilerinin saptanması amaçlanmıştır. Bu amaç için Kırıkhan-Reyhanlı bölgesi tarım topraklarını temsil edecek şekilde iki farklı derinlik (0-20 ve 20-40 cm) ve 30 ayrı noktadan olmak üzere toplamda 60 toprak örneği alınmıştır. Alınan toprak örneklerinde; Kadmiyum (Cd), Cobalt (Co), Krom (Cr), Nikel (Ni), Bakır (Cu), Demir (Fe) ve Molibden (Mo) içerikleri belirlenmiştir. Araştırma sonuçlarına göre; toprakların Cd içerikleri 0.009-0.041 µg/kg; Co içeriği 0.011-0.317 µg/kg; Cr 0.008-0.187 µg/kg, Ni içerikleri 0.787-6.211 ppm; Cu içerikleri 1.11-3.77 ppm; Fe içerikleri 2.80-15.09 ve Mo içerikleri 0.006-0.101 µg/kg arasında bulunmuştur. Toprakların Mo ile Co ve Ni içerikleri arasında pozitif önemli ilişkiler belirlenirken Cr içeriği ile negatif önemli ilişkiler belirlenmiştir. Aynı zamanda Cd ile Co ve Ni; Co ile Ni ve Cu ile Fe aralarında ise pozitif önemli ilişkiler belirlenmiştir. Bölge topraklarının ağır metal içerikleri sınır değerler ile karşılaştırıldığında herhangi bir ağır metal kirliliğine rastlanmamıştır.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Son yıllarda bitkilerin besinsel bileşenleri, sağlık üzerindeki etkileri nedeniyle pek çok çalışmalara konu olmuştur. Bu çalışmada Moringa oleifera yaprak tozunda mineral ve yağ asidi içeriği belirlenmiştir. Element analizi için numuneler, bir mikrodalga fırında HNO3 ve HCl ile sindirildi. Numunelerde mineral konsantrasyonları İndüktif Eşleşmiş Plazma Kütle Spektrometresi (ICP-MS) ile belirlendi. Numunelerde magnezyum 6393 μg g-1, demir 927 μg g-1, çinko 138 μg g-1 ve bakır ise 6 μg g-1 olarak tespit edilmiştir. Yağ asiti ekstraksiyonu sonrası, yağ asidi ve ester profilleri için Gaz Kromatografisi Alev İyonlaşmalı Dedektörü (GC-FID) kullanılarak tanımlanmıştır. Moringa oleifera yaprağında yağ asitlerinin ana bileşenleri olarak lauric asit (C12:0), tridekanoik asit (C13:0), miristik asit (C14:0), palmitik asit (C16:0), stearik asit (C18:0), oleik asit (C18:1), linoleik asit (C18:2), gama-linolenik asit (C18:3) tayin edilmiştir.
{"title":"Moringa oleifera bitki yaprağının mineral ve yağ asidi bileşenlerinin belirlenmesi","authors":"Serpil Kiliç, Murat Kiliç","doi":"10.46239/ejbcs.1227956","DOIUrl":"https://doi.org/10.46239/ejbcs.1227956","url":null,"abstract":"Son yıllarda bitkilerin besinsel bileşenleri, sağlık üzerindeki etkileri nedeniyle pek çok çalışmalara konu olmuştur. Bu çalışmada Moringa oleifera yaprak tozunda mineral ve yağ asidi içeriği belirlenmiştir. Element analizi için numuneler, bir mikrodalga fırında HNO3 ve HCl ile sindirildi. Numunelerde mineral konsantrasyonları İndüktif Eşleşmiş Plazma Kütle Spektrometresi (ICP-MS) ile belirlendi. Numunelerde magnezyum 6393 μg g-1, demir 927 μg g-1, çinko 138 μg g-1 ve bakır ise 6 μg g-1 olarak tespit edilmiştir. Yağ asiti ekstraksiyonu sonrası, yağ asidi ve ester profilleri için Gaz Kromatografisi Alev İyonlaşmalı Dedektörü (GC-FID) kullanılarak tanımlanmıştır. Moringa oleifera yaprağında yağ asitlerinin ana bileşenleri olarak lauric asit (C12:0), tridekanoik asit (C13:0), miristik asit (C14:0), palmitik asit (C16:0), stearik asit (C18:0), oleik asit (C18:1), linoleik asit (C18:2), gama-linolenik asit (C18:3) tayin edilmiştir.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126041011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried-out: a) to develop a high-performance liquid chromatography with size exclusion column (HPLC-SEC) method for the identification of the major whey proteins from selected whey protein products; and b) use the method to estimate the relative composition of the major whey proteins in different whey protein products. An HPLC Shimadzu (LC-10AD VP liquid chromatograph) with system controller (SCL-10A VP) equipped with a pump and auto-injector (SIL-10AD VP) and UV-vis detector (SPD-10AV) was used in the identification of whey proteins in standards and whey protein products. The size exclusion column (SEC) was a Yarra 3 µm, SEC-3000 Column, 7.8 mm I.D. x 30 cm with a security guard. The HPLC-SEC method was successful in identifying the major whey proteins of the different whey protein products. The β-LG contents had the highest level among the whey proteins for all the whey protein products studied, followed by the α-LA and then IgG in both WPC products. However, the α-LA and IgG of the Procream product had almost the same level which was probably due to a different process used in WPC All the major whey proteins with the highest pump flowrate had the shortest elution times while the whey proteins with the lowest pump flowrate had the longest elution times. The optimal pump flowrate was 0.75 mL/min since it gave a faster analysis but differentiate the peaks of the different major whey proteins.
本研究的目的是:a)建立一种高效液相色谱-粒径排除柱(HPLC-SEC)方法，用于从选定的乳清蛋白产品中鉴定主要乳清蛋白;b)利用该方法估算不同乳清蛋白制品中主要乳清蛋白的相对组成。采用岛津HPLC (LC-10AD VP)液相色谱仪，系统控制器(SCL-10A VP)配泵和自进样器(SIL-10AD VP)和紫外可见检测器(SPD-10AV)，对乳清蛋白标准品和乳清蛋白制品进行鉴定。尺寸排除柱(SEC)为Yarra 3µm, SEC-3000柱，7.8 mm id x 30 cm，配有保安。HPLC-SEC方法可以成功地鉴定不同乳清蛋白产品的主要乳清蛋白。在所有乳清蛋白产品中，β-LG含量最高，α-LA次之，IgG含量最高。而Procream产品的α-LA和IgG含量几乎相同，这可能与WPC工艺不同有关。泵送流量最大的乳清蛋白洗脱时间最短，泵送流量最小的乳清蛋白洗脱时间最长。最佳泵流量为0.75 mL/min，因为它可以更快地分析，但区分了不同主要乳清蛋白的峰。
{"title":"High Performance Liquid Chromatography with Size Exclusion Column (HPLC-SEC) Method for Identifying the Major Whey Proteins of Whey Protein Products","authors":"L. Diamante","doi":"10.46239/ejbcs.1143057","DOIUrl":"https://doi.org/10.46239/ejbcs.1143057","url":null,"abstract":"This study was carried-out: a) to develop a high-performance liquid chromatography with size exclusion column (HPLC-SEC) method for the identification of the major whey proteins from selected whey protein products; and b) use the method to estimate the relative composition of the major whey proteins in different whey protein products. \u0000An HPLC Shimadzu (LC-10AD VP liquid chromatograph) with system controller (SCL-10A VP) equipped with a pump and auto-injector (SIL-10AD VP) and UV-vis detector (SPD-10AV) was used in the identification of whey proteins in standards and whey protein products. The size exclusion column (SEC) was a Yarra 3 µm, SEC-3000 Column, 7.8 mm I.D. x 30 cm with a security guard. \u0000The HPLC-SEC method was successful in identifying the major whey proteins of the different whey protein products. \u0000The β-LG contents had the highest level among the whey proteins for all the whey protein products studied, followed by the α-LA and then IgG in both WPC products. However, the α-LA and IgG of the Procream product had almost the same level which was probably due to a different process used in WPC \u0000All the major whey proteins with the highest pump flowrate had the shortest elution times while the whey proteins with the lowest pump flowrate had the longest elution times. The optimal pump flowrate was 0.75 mL/min since it gave a faster analysis but differentiate the peaks of the different major whey proteins.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"55 80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124774504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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