Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400023
Rosa Maria Lizana Ballve, H. Medina-Filho, Rita Bordignon
Broadness of leaf petiole wing (WB) was investigated as a morphological marker for screening hybrids of the very narrow-winged species Citrus limonia and C. sunki with broad-winged species C. aurantium and C. sinensis. Controlled polinizations produced over 500 reciprocal hybrids with potential in the ongoing rootstock breeding program identified by the Pgi-1 and Prxa-1 isozyme loci. Measurement ratios WB/leaf length, WB/leaf broadness and WB/petiole length identified 86 to 91% of the reciprocal hybrids produced. However, visual classification of WB was an equally efficient but much easier and faster method. It can be very useful in breeding programs when large number of plants have to be screened or when isozyme, RFLP or RAPD laboratories are not available.
{"title":"Identification of reciprocal hybrids in citrus by the broadness of the leaf petiole wing","authors":"Rosa Maria Lizana Ballve, H. Medina-Filho, Rita Bordignon","doi":"10.1590/S0100-84551997000400023","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400023","url":null,"abstract":"Broadness of leaf petiole wing (WB) was investigated as a morphological marker for screening hybrids of the very narrow-winged species Citrus limonia and C. sunki with broad-winged species C. aurantium and C. sinensis. Controlled polinizations produced over 500 reciprocal hybrids with potential in the ongoing rootstock breeding program identified by the Pgi-1 and Prxa-1 isozyme loci. Measurement ratios WB/leaf length, WB/leaf broadness and WB/petiole length identified 86 to 91% of the reciprocal hybrids produced. However, visual classification of WB was an equally efficient but much easier and faster method. It can be very useful in breeding programs when large number of plants have to be screened or when isozyme, RFLP or RAPD laboratories are not available.","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117154955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400001
Marta Elisabeth Valim-Labres, M. D. M. Porto, A. Matsumura
Nine isolates of Bipolaris sorokiniana were inoculated on three cultivars of wheat plants (susceptible, moderately resistant, resistant). Eight days after the inoculation, the isolates were recovered (27 isolates) and the following isozymatic patterns were analyzed: esterase, alkaline phosphatase, acid phosphatase, malate dehydrogenase, and superoxide dismutase. The esterase system was the most polymorphic, and the isolates recovered from the susceptible cultivar showed the highest variability. This is evidence that this cultivar exerts low selection pressure on the pathogen
{"title":"Effects of host resistance on the isozymatic patterns of Bipolaris sorokiniana (Dematiaceae, Moniliales)","authors":"Marta Elisabeth Valim-Labres, M. D. M. Porto, A. Matsumura","doi":"10.1590/S0100-84551997000400001","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400001","url":null,"abstract":"Nine isolates of Bipolaris sorokiniana were inoculated on three cultivars of wheat plants (susceptible, moderately resistant, resistant). Eight days after the inoculation, the isolates were recovered (27 isolates) and the following isozymatic patterns were analyzed: esterase, alkaline phosphatase, acid phosphatase, malate dehydrogenase, and superoxide dismutase. The esterase system was the most polymorphic, and the isolates recovered from the susceptible cultivar showed the highest variability. This is evidence that this cultivar exerts low selection pressure on the pathogen","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126676155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400005
D. Selivon, J. S. Morgante
{"title":"Reproductive isolation between Anastrepha bistrigata and A. striata (Diptera, Tephritidae)","authors":"D. Selivon, J. S. Morgante","doi":"10.1590/S0100-84551997000400005","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400005","url":null,"abstract":"","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129173059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400030
Sílvia R.P. Miranda, S. F. Fonseca, M. Figueiredo, M. Yamamoto, H. Grotto, S. Saad, F. Costa
Hb Koln resulta da substituicao do aminoacido valina por metionina na posicao 98 da cadeia da hemoglobina. Embora seja a mais frequente das hemoglobinas instaveis, possui poucas caracteristicas que permitem distinguila sem a realizacao de analise estrutural da molecula. Com o objetivo de desenvolver um procedimento alternativo a analise proteica, este trabalho descreve a identificacao da Hb Koln atraves de analise de DNA. Uma paciente branca, sexo feminino, 4 anos de idade, com ictericia desde o nascimento, foi analisada, apresentando uma banda anomala entre A2 e S em eletroforese em acetato de celulose, e migracao eletroforetica semelhante a Hb C em gel de agar. Os testes de instabilidade termica e de precipitacao pelo isopropanol foram positivos e foram observados corpusculos de Heinz no sangue periferico. Os tres exons do gene da globina foram sequenciados e uma transicao de G ® A foi identificada na primeira posicao do codon 98. Essa alteracao nao cria ou abole nenhum sitio conhecido de enzima de restricao. Desta forma, a confirmacao da mutacao foi levada a efeito atraves de hibridizacao com oligonucleotideos aleloespecificos, o que permitiu o estabelecimento de um metodo simples e rapido de identificacao de novos casos quando os dados clinicos e o padrao hematologico e eletroforetico sugerem Hb Koln.
{"title":"Hb Köln [ a 2 b 298(FG5) val-met] identified by DNA analysis in a Brazilian family","authors":"Sílvia R.P. Miranda, S. F. Fonseca, M. Figueiredo, M. Yamamoto, H. Grotto, S. Saad, F. Costa","doi":"10.1590/S0100-84551997000400030","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400030","url":null,"abstract":"Hb Koln resulta da substituicao do aminoacido valina por metionina na posicao 98 da cadeia da hemoglobina. Embora seja a mais frequente das hemoglobinas instaveis, possui poucas caracteristicas que permitem distinguila sem a realizacao de analise estrutural da molecula. Com o objetivo de desenvolver um procedimento alternativo a analise proteica, este trabalho descreve a identificacao da Hb Koln atraves de analise de DNA. Uma paciente branca, sexo feminino, 4 anos de idade, com ictericia desde o nascimento, foi analisada, apresentando uma banda anomala entre A2 e S em eletroforese em acetato de celulose, e migracao eletroforetica semelhante a Hb C em gel de agar. Os testes de instabilidade termica e de precipitacao pelo isopropanol foram positivos e foram observados corpusculos de Heinz no sangue periferico. Os tres exons do gene da globina foram sequenciados e uma transicao de G ® A foi identificada na primeira posicao do codon 98. Essa alteracao nao cria ou abole nenhum sitio conhecido de enzima de restricao. Desta forma, a confirmacao da mutacao foi levada a efeito atraves de hibridizacao com oligonucleotideos aleloespecificos, o que permitiu o estabelecimento de um metodo simples e rapido de identificacao de novos casos quando os dados clinicos e o padrao hematologico e eletroforetico sugerem Hb Koln.","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127055940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400022
A. R. Neto, L. L. Nass, J. B. M. Filho
Temperate and temperate x tropical germplasms were introduced into the maize breeding of the Department of Genetics (ESALQ-USP). Twenty populations designated as P-1 to P-20 and a check population were evaluated in a randomized complete block design with two replications at Piracicaba, SP, Brazil. Plots were 2.0 m long, spaced 1.0 m apart, with 10 plants per plot after thinning. The following traits were evaluated: PH, plant height; EH, ear height; TB, tassel branch number; KW, 100 kernels weight; EL, ear length; ED, ear diameter; KR, kernels per row, and RN, number of rows of kernels. The means of the exotic populations showed a variation in performance for all traits. The population means were greater than check means for KW, EL, and KR. Populations P-9, P-10, P-12, and P-13 showed low relative ear placement values (EH/PH index), indicating that these materials could contribute to lower ear placement in local populations. P-1 and P-9 showed a high potential to reduce TB. The phenotypic correlation coefficients among populations were positive for most combinations, except for RN with PH, EH, TB, and KW. Predictions of 19 composite means were obtained for all traits.
{"title":"Potential of twenty exotic germplasms to improve Brazilian maize architecture","authors":"A. R. Neto, L. L. Nass, J. B. M. Filho","doi":"10.1590/S0100-84551997000400022","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400022","url":null,"abstract":"Temperate and temperate x tropical germplasms were introduced into the maize breeding of the Department of Genetics (ESALQ-USP). Twenty populations designated as P-1 to P-20 and a check population were evaluated in a randomized complete block design with two replications at Piracicaba, SP, Brazil. Plots were 2.0 m long, spaced 1.0 m apart, with 10 plants per plot after thinning. The following traits were evaluated: PH, plant height; EH, ear height; TB, tassel branch number; KW, 100 kernels weight; EL, ear length; ED, ear diameter; KR, kernels per row, and RN, number of rows of kernels. The means of the exotic populations showed a variation in performance for all traits. The population means were greater than check means for KW, EL, and KR. Populations P-9, P-10, P-12, and P-13 showed low relative ear placement values (EH/PH index), indicating that these materials could contribute to lower ear placement in local populations. P-1 and P-9 showed a high potential to reduce TB. The phenotypic correlation coefficients among populations were positive for most combinations, except for RN with PH, EH, TB, and KW. Predictions of 19 composite means were obtained for all traits.","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126738982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400025
M. J. Valarini, I. Otsuk, M. L. C. Vieira
Plants were regenerated from leaf-derived callus culture of Stylosanthes scabra, a polyploid legume tolerant to drought and adapted to acid soils. A total of 168 regenerants were planted out in Leonard jars in a complete randomized design. Nitrogen fixation and vegetative growth were indirectly evaluated by shoot dry weight, root dry weight, shoot N content and acetylene reduction activity. The results showed higher variation in the regenerants than in controls not submitted to tissue culture. Significant differences were found for all nitrogen fixation related-traits
{"title":"Changes in N2 fixation in Stylosanthes scabra derived from tissue culture","authors":"M. J. Valarini, I. Otsuk, M. L. C. Vieira","doi":"10.1590/S0100-84551997000400025","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400025","url":null,"abstract":"Plants were regenerated from leaf-derived callus culture of Stylosanthes scabra, a polyploid legume tolerant to drought and adapted to acid soils. A total of 168 regenerants were planted out in Leonard jars in a complete randomized design. Nitrogen fixation and vegetative growth were indirectly evaluated by shoot dry weight, root dry weight, shoot N content and acetylene reduction activity. The results showed higher variation in the regenerants than in controls not submitted to tissue culture. Significant differences were found for all nitrogen fixation related-traits","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121717132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400002
A. Balan, G. Padilla
Dois novos fagos de Streptomyces, denominados oBP1 e oBP2, foram isolados de amostras de solos tropicais. Estes fagos apresentaram uma grande faixa de hospedeiros e foram capazes de desenvolver os ciclos litico e lisogenico em diferentes especies de Streptomyces testadas. A temperatura de incubacao mostrou-se importante para o desenvolvimento do ciclo de replicacao. O aumento desta temperatura de 30oC para 37oC ou 42oC induziu o ciclo litico de oBP2 e lisogenico de oBP1 na linhagem hospedeira Streptomyces sp. WL6. Ambos os fagos apresentaram cabeca icosaedrica com talos longos e foram caracterizados em relacao a morfologia, tamanho do genoma, conteudo de G + C e curva de adsorcao
{"title":"New thermal inducible Streptomyces phages isolated from tropical soils","authors":"A. Balan, G. Padilla","doi":"10.1590/S0100-84551997000400002","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400002","url":null,"abstract":"Dois novos fagos de Streptomyces, denominados oBP1 e oBP2, foram isolados de amostras de solos tropicais. Estes fagos apresentaram uma grande faixa de hospedeiros e foram capazes de desenvolver os ciclos litico e lisogenico em diferentes especies de Streptomyces testadas. A temperatura de incubacao mostrou-se importante para o desenvolvimento do ciclo de replicacao. O aumento desta temperatura de 30oC para 37oC ou 42oC induziu o ciclo litico de oBP2 e lisogenico de oBP1 na linhagem hospedeira Streptomyces sp. WL6. Ambos os fagos apresentaram cabeca icosaedrica com talos longos e foram caracterizados em relacao a morfologia, tamanho do genoma, conteudo de G + C e curva de adsorcao","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116293822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400017
B. Goldschmidt, Denise M Nogueira, Denise Wilches Monsores, Lucia Moreno de Souza
The karyotypes of two species of Psittacidae of the genus Aratinga, Aratinga guarouba and A. acuticaudata were studied for the first time. The metaphases were obtained using a short term culture of feather pulp. These karyotypes are compared with others of the genus and their karyological relationships in the Psittaciformes are discussed
{"title":"Chromosome study in two Aratinga species (A. guarouba and A. acuticaudata) (Psittaciformes)","authors":"B. Goldschmidt, Denise M Nogueira, Denise Wilches Monsores, Lucia Moreno de Souza","doi":"10.1590/S0100-84551997000400017","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400017","url":null,"abstract":"The karyotypes of two species of Psittacidae of the genus Aratinga, Aratinga guarouba and A. acuticaudata were studied for the first time. The metaphases were obtained using a short term culture of feather pulp. These karyotypes are compared with others of the genus and their karyological relationships in the Psittaciformes are discussed","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127250109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400033
B. Beiguelman
{"title":"Genetic disease and the development of Human Genetics","authors":"B. Beiguelman","doi":"10.1590/S0100-84551997000400033","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400033","url":null,"abstract":"","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127000269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-12-01DOI: 10.1590/S0100-84551997000400010
É. M. Rabelo, J. Tata
E bem estabelecido que virtualmente todos os tecidos das larvas de anfibios sao altamente sensiveis a acao mutuamente antagonista dos hormonios tireoideanos (TH) e prolactina (PRL). Porem, nao e sabido se os tecidos do anfibio adulto respondem de uma forma semelhante a estes hormonios. Em um trabalho anterior, nos demonstramos que baixas doses de triiodotironina (T3) potenciam rapidamente e com alta intensidade a ativacao pelo estrogenio (E2) de genes silenciosos da vitelogenina (Vit), bem como a autoinducao de transcritos de receptores para estrogenio (ER) em culturas primarias de hepatocitos de Xenopus adultos. Esta resposta para o T3 e acompanhada pela ativacao da transcricao do mRNA de receptores b para o hormonio tireoideano (TRb). Atraves das tecnicas de Northern blotting e "RNase protection assay", nos agora estamos mostrando que prolactina ovina adicionada por 12 h, juntamente com 2 x 10-9 M de T3, em culturas primarias de hepatocitos adultos de Xenopus bloqueia completamente a acao de T3 na potenciacao da inducao de mRNA da Vit pelo hormonio E2. PRL tambem aboliu a autoregulacao positiva do mRNA para TRb e a ativacao cruzada da autoinducao do mRNA do ER. Assim, nos estamos mostrando pela primeira vez que a acao anti-TH da PRL, manifestada em girinos de Xenopus durante a metamorfose, e mantida no figado adulto, sugerindo que esta acao mutuamente antagonista dos dois hormonios pode ser desempenhada por mecanismos moleculares semelhantes nos tecidos de ambos os estagios do anfibio.
众所周知，几乎所有的两栖动物幼虫组织都对甲状腺激素(TH)和催乳素(PRL)的相互拮抗作用高度敏感。然而，目前尚不清楚成年两栖动物的组织是否对这些激素有类似的反应。在之前的研究中，我们证明了低剂量的三碘甲状腺原氨酸(T3)可以快速、高强度地增强卵黄原氨酸(Vit)沉默基因的雌激素(E2)激活，以及雌激素受体(ER)转录本的自诱导。这种对T3的反应伴随着甲状腺激素b受体mRNA转录的激活。通过blotting北部的方法和核糖核酸酶保护试验”,我们现在显示了催乳素增加到12点,再加上2 x 10 M T3的成人肝细胞的原始文化的非洲爪蟾蜍块完全的T3的明星在potenciacao inducao mRNA的维特的激素E2。PRL还抑制了TRb mRNA的正自调节和ER mRNA自诱导的交叉激活。因此，我们首次证明PRL的抗th作用，在非洲爪蟾蝌蚪蜕变期间表现出来，并在成虫中维持，这表明这两种激素的相互拮抗作用可能是通过类似的分子机制在两栖动物的两个阶段的组织中发挥的。
{"title":"Prolactin inhibits auto- and cross-induction of thyroid hormone and estrogen receptor and vitellogenin genes in adult Xenopus (Amphibia) hepatocytes","authors":"É. M. Rabelo, J. Tata","doi":"10.1590/S0100-84551997000400010","DOIUrl":"https://doi.org/10.1590/S0100-84551997000400010","url":null,"abstract":"E bem estabelecido que virtualmente todos os tecidos das larvas de anfibios sao altamente sensiveis a acao mutuamente antagonista dos hormonios tireoideanos (TH) e prolactina (PRL). Porem, nao e sabido se os tecidos do anfibio adulto respondem de uma forma semelhante a estes hormonios. Em um trabalho anterior, nos demonstramos que baixas doses de triiodotironina (T3) potenciam rapidamente e com alta intensidade a ativacao pelo estrogenio (E2) de genes silenciosos da vitelogenina (Vit), bem como a autoinducao de transcritos de receptores para estrogenio (ER) em culturas primarias de hepatocitos de Xenopus adultos. Esta resposta para o T3 e acompanhada pela ativacao da transcricao do mRNA de receptores b para o hormonio tireoideano (TRb). Atraves das tecnicas de Northern blotting e \"RNase protection assay\", nos agora estamos mostrando que prolactina ovina adicionada por 12 h, juntamente com 2 x 10-9 M de T3, em culturas primarias de hepatocitos adultos de Xenopus bloqueia completamente a acao de T3 na potenciacao da inducao de mRNA da Vit pelo hormonio E2. PRL tambem aboliu a autoregulacao positiva do mRNA para TRb e a ativacao cruzada da autoinducao do mRNA do ER. Assim, nos estamos mostrando pela primeira vez que a acao anti-TH da PRL, manifestada em girinos de Xenopus durante a metamorfose, e mantida no figado adulto, sugerindo que esta acao mutuamente antagonista dos dois hormonios pode ser desempenhada por mecanismos moleculares semelhantes nos tecidos de ambos os estagios do anfibio.","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"410 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123964391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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