Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300009
C. Grisolia, Í. Ferrari
O herbicida metolachlor foi avaliado quanto ao seu potencial genotoxico. Em camundongos nao houve inducao de micronucleos nos eritrocitos policromaticos de medula ossea; entretanto, com a dose mais forte de 40 mg/kg peso por 48 h, observaram-se efeitos citotoxicos atraves da queda acentuada na porcentagem de eritrocitos policromaticos em relacao aos normocromaticos. O metolachlor tambem nao induziu aberracoes cromossomicas em linfocitos humanos quando tratados in vitro, porem na concentracao de 2,0 mg/ml de meio ocorreu queda no indice mitotico, demonstrando a toxidez do tratamento. Para se estudar os efeitos genotoxicos de possiveis metabolitos do metolachlor, ratos Wistar foram tratados com duas doses de 50 mg/kg com um intervalo de 24 h. Uma hora apos o ultimo tratamento, seus plasmas foram coletados atraves de puncao cardiaca e adicionados as culturas de linfocitos humanos. Os resultados tambem demonstraram efeitos citotoxicos mas nao de mutagenicidade
{"title":"In vitro and in vivo studies demonstrate non-mutagenicity of the herbicide metolachlor","authors":"C. Grisolia, Í. Ferrari","doi":"10.1590/S0100-84551997000300009","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300009","url":null,"abstract":"O herbicida metolachlor foi avaliado quanto ao seu potencial genotoxico. Em camundongos nao houve inducao de micronucleos nos eritrocitos policromaticos de medula ossea; entretanto, com a dose mais forte de 40 mg/kg peso por 48 h, observaram-se efeitos citotoxicos atraves da queda acentuada na porcentagem de eritrocitos policromaticos em relacao aos normocromaticos. O metolachlor tambem nao induziu aberracoes cromossomicas em linfocitos humanos quando tratados in vitro, porem na concentracao de 2,0 mg/ml de meio ocorreu queda no indice mitotico, demonstrando a toxidez do tratamento. Para se estudar os efeitos genotoxicos de possiveis metabolitos do metolachlor, ratos Wistar foram tratados com duas doses de 50 mg/kg com um intervalo de 24 h. Uma hora apos o ultimo tratamento, seus plasmas foram coletados atraves de puncao cardiaca e adicionados as culturas de linfocitos humanos. Os resultados tambem demonstraram efeitos citotoxicos mas nao de mutagenicidade","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127915755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300015
M. Penteado, P. García, M. P. Vega
Isozyme patterns and their genetic control in three Centrosema species are described. Seven isozymatic systems (aspartate aminotransferase, glucose-6-phosphate isomerase, phosphoglucomutase, anodal peroxidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and isocitrate dehydrogenase) were studied in 18 populations and several breeding lines of C. acutifolium, C. brasilianum and C. pubescens, using starch gel electrophoresis techniques. All systems, except glucose-6-phosphate isomerase, are described for the first time in these species. A total of 17 isozyme loci were scored; this represents the largest set of Mendelian loci known up to now in Centrosema species. Isozyme polymorphism and variability within and between populations and species were relatively high and allowed discrimination among species
{"title":"Isozyme markers and genetic variability in three species of Centrosema (Leguminosae)","authors":"M. Penteado, P. García, M. P. Vega","doi":"10.1590/S0100-84551997000300015","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300015","url":null,"abstract":"Isozyme patterns and their genetic control in three Centrosema species are described. Seven isozymatic systems (aspartate aminotransferase, glucose-6-phosphate isomerase, phosphoglucomutase, anodal peroxidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and isocitrate dehydrogenase) were studied in 18 populations and several breeding lines of C. acutifolium, C. brasilianum and C. pubescens, using starch gel electrophoresis techniques. All systems, except glucose-6-phosphate isomerase, are described for the first time in these species. A total of 17 isozyme loci were scored; this represents the largest set of Mendelian loci known up to now in Centrosema species. Isozyme polymorphism and variability within and between populations and species were relatively high and allowed discrimination among species","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133501148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300024
M. Quoirin, F. Aragão, E. Rech, D. Oliveira
We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues
{"title":"Transient expression of a reporter gene introduced by bioballistic bombardment into Racosperma mangium (Leguminosae family) tissues","authors":"M. Quoirin, F. Aragão, E. Rech, D. Oliveira","doi":"10.1590/S0100-84551997000300024","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300024","url":null,"abstract":"We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115247901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300027
B. Beiguelman
{"title":"Genética, Ética e Estado: (Genetics, Ethics and State)","authors":"B. Beiguelman","doi":"10.1590/S0100-84551997000300027","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300027","url":null,"abstract":"","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123371745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300023
L. Chaves, J. B. M. Filho
Prediction of variety composite means was shown to be feasible without diallel crossing the parental varieties. Thus, the predicted mean for a quantitative trait of a composite is given by: Yk = a1 SVj + a2STj + a3 - a4, with coefficients a1 = (n - 2k)/k2(n - 2); a2 = 2n(k - 1)/k2(n - 2); a3 = n(k - 1)/k(n - 1)(n - 2); and a4 = n2(k - 1)/k(n - 1)(n - 2); summation is for j = 1 to k, where k is the size of the composite (number of parental varieties of a particular composite) and n is the total number of parent varieties. Vj is the mean of varieties and Tj is the mean of topcrosses (pool of varieties as tester), and and are the respective average values in the whole set. Yield data from a 7 x 7 variety diallel cross were used for the variety means and for the "simulated" topcross means to illustrate the proposed procedure. The proposed prediction procedure was as effective as the prediction based on Yk = - ( -)/k, where and refer to the mean of hybrids (F1) and parental varieties, respectively, in a variety diallel cross. It was also shown in the analysis of variance that the total sum of squares due to treatments (varieties and topcrosses) can be orthogonally partitioned following the reduced model Yjj = m + ½(vj + vj) + + hj+ hj, thus making possible an F test for varieties, average heterosis and variety heterosis. Least square estimates of these effects are also given
{"title":"Predicting variety composite means without diallel crossing","authors":"L. Chaves, J. B. M. Filho","doi":"10.1590/S0100-84551997000300023","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300023","url":null,"abstract":"Prediction of variety composite means was shown to be feasible without diallel crossing the parental varieties. Thus, the predicted mean for a quantitative trait of a composite is given by: Yk = a1 SVj + a2STj + a3 - a4, with coefficients a1 = (n - 2k)/k2(n - 2); a2 = 2n(k - 1)/k2(n - 2); a3 = n(k - 1)/k(n - 1)(n - 2); and a4 = n2(k - 1)/k(n - 1)(n - 2); summation is for j = 1 to k, where k is the size of the composite (number of parental varieties of a particular composite) and n is the total number of parent varieties. Vj is the mean of varieties and Tj is the mean of topcrosses (pool of varieties as tester), and and are the respective average values in the whole set. Yield data from a 7 x 7 variety diallel cross were used for the variety means and for the \"simulated\" topcross means to illustrate the proposed procedure. The proposed prediction procedure was as effective as the prediction based on Yk = - ( -)/k, where and refer to the mean of hybrids (F1) and parental varieties, respectively, in a variety diallel cross. It was also shown in the analysis of variance that the total sum of squares due to treatments (varieties and topcrosses) can be orthogonally partitioned following the reduced model Yjj = m + ½(vj + vj) + + hj+ hj, thus making possible an F test for varieties, average heterosis and variety heterosis. Least square estimates of these effects are also given","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124918556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300010
V. E. R. D. Celis, D. N. Gassen, M. Santos-Colares, Alice Kalisz de Oliveira, V. Valente
We examined the chromosome set of the aphid species Sitobion avenae, Schizaphis graminum and Methopolophium dirhodum by means of conventional staining and C, NOR, AluI and HaeIII banding methods. These species are considered important pests to several plants of economic interest in Brazil. No variation was observed in the number of chromosomes of S. avenae, whereas there was intraspecific variation in the other two species. Interspecific differences in the response to the banding treatments were observed. Whereas these techniques allowed the identification of several S. graminum chromosome pairs, only the AluI treatment was capable of inducing differential staining in the M. dirhodum chromosomes and no clear patterns emerged when the S. avenae preparations were treated
{"title":"Chromosome studies in southern Brazilian wheat pest aphids Sitobion avenae, Schizaphis graminum, and Methopolophium dirhodum (Homoptera: Aphididae)","authors":"V. E. R. D. Celis, D. N. Gassen, M. Santos-Colares, Alice Kalisz de Oliveira, V. Valente","doi":"10.1590/S0100-84551997000300010","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300010","url":null,"abstract":"We examined the chromosome set of the aphid species Sitobion avenae, Schizaphis graminum and Methopolophium dirhodum by means of conventional staining and C, NOR, AluI and HaeIII banding methods. These species are considered important pests to several plants of economic interest in Brazil. No variation was observed in the number of chromosomes of S. avenae, whereas there was intraspecific variation in the other two species. Interspecific differences in the response to the banding treatments were observed. Whereas these techniques allowed the identification of several S. graminum chromosome pairs, only the AluI treatment was capable of inducing differential staining in the M. dirhodum chromosomes and no clear patterns emerged when the S. avenae preparations were treated","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121276116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300006
F. L. Dias, L. M. Antunes, C. Takahashi
Combined therapy with radiation and chemotherapy has being increasingly used in cancer treatment. The effect of combinations of taxol (0.08 mg/ml) with doxorubicin (DXR, 0.5 or 1.0 mg/ml) or gamma radiation (20 or 40 cGy) was examined in two different treatment schedules (pretreatment or simultaneous treatment) using Chinese hamster ovary (CHO) cells treated at the G2 phase of the cell cycle. The results showed that taxol did not have a radiosensitizing effect on the chromosomal aberrations induced by gamma radiation nor did it have a potentiating effect on the chromosomal aberrations induced by DXR in CHO cells treated in the G2 phase of the cell cycle
{"title":"Effect of taxol on chromosome aberrations induced by gamma radiation or by doxorubicin in Chinese hamster ovary cells","authors":"F. L. Dias, L. M. Antunes, C. Takahashi","doi":"10.1590/S0100-84551997000300006","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300006","url":null,"abstract":"Combined therapy with radiation and chemotherapy has being increasingly used in cancer treatment. The effect of combinations of taxol (0.08 mg/ml) with doxorubicin (DXR, 0.5 or 1.0 mg/ml) or gamma radiation (20 or 40 cGy) was examined in two different treatment schedules (pretreatment or simultaneous treatment) using Chinese hamster ovary (CHO) cells treated at the G2 phase of the cell cycle. The results showed that taxol did not have a radiosensitizing effect on the chromosomal aberrations induced by gamma radiation nor did it have a potentiating effect on the chromosomal aberrations induced by DXR in CHO cells treated in the G2 phase of the cell cycle","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133342640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-09-01DOI: 10.1590/S0100-84551997000300019
R. Barbieri, F. Carvalho, A. Dornelles, R. M. O. D. Cristaldo, C. Handel
Six wheat genotypes and their F1 and F2 generations were exposed to the action of Helminthosporium sativum culture filtrates to examine the genetics of hexaploid wheat resistance. The objective was to improve the efficiency of breeding programs by identifying the action and number of genes involved in the resistance. The varied response of the tested genotypes to the culture filtrates allowed division of the genotypes into four groups: resistant, moderately resistant, moderately susceptible and susceptible. This variability was detected in the progeny, suggesting that the parents have distinct genetic constitutions. Additive gene action predominated and genetic gain was shown to be possible through selection. The genetic control of the resistance trait seems to be complex because of the presence of gene interaction and the difficulty of eliminating the environmental effects. The inheritance seems to be oligogenic
{"title":"Genetics of resistance to the fungus Helminthosporium sativum in wheat: use of culture filtrates in tissue culture","authors":"R. Barbieri, F. Carvalho, A. Dornelles, R. M. O. D. Cristaldo, C. Handel","doi":"10.1590/S0100-84551997000300019","DOIUrl":"https://doi.org/10.1590/S0100-84551997000300019","url":null,"abstract":"Six wheat genotypes and their F1 and F2 generations were exposed to the action of Helminthosporium sativum culture filtrates to examine the genetics of hexaploid wheat resistance. The objective was to improve the efficiency of breeding programs by identifying the action and number of genes involved in the resistance. The varied response of the tested genotypes to the culture filtrates allowed division of the genotypes into four groups: resistant, moderately resistant, moderately susceptible and susceptible. This variability was detected in the progeny, suggesting that the parents have distinct genetic constitutions. Additive gene action predominated and genetic gain was shown to be possible through selection. The genetic control of the resistance trait seems to be complex because of the presence of gene interaction and the difficulty of eliminating the environmental effects. The inheritance seems to be oligogenic","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131510104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1997-03-01DOI: 10.1590/S0100-84551997000100007
C. L. Souza-Junior, Jim Fernandes
{"title":"Predicting the range of inbreeding depression of inbred lines in cross-pollinated populations","authors":"C. L. Souza-Junior, Jim Fernandes","doi":"10.1590/S0100-84551997000100007","DOIUrl":"https://doi.org/10.1590/S0100-84551997000100007","url":null,"abstract":"","PeriodicalId":340356,"journal":{"name":"Brazilian Journal of Genetics","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123887336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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