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Correlation between high molecular weight gluten subunits composition and bread-making quality in Brazilian wheat 巴西小麦高分子量面筋亚基组成与面包品质的相关性
Pub Date : 1997-12-01 DOI: 10.1590/S0100-84551997000400019
I. Schuster, M. A. Souza, A. Cardoso, C. Sediyama, M. Moreira
Bread-making quality is one of the most important targets in the genetic improvement of wheat. Although extensive analyses of quality traits such as farinography, sodium dodecyl sulfate (SDS) sedimentation, alveography, and baking are made in breeding programs, these analyses require high amounts of seeds which are obtained only in late generations. In this experiment the statistical correlations between the high molecular weight subunit of glutenin and bread-making quality measured by alveograph, farinograph and SDS sedimentation were evaluated. Seventeen wheat genotypes were grown under the same conditions, each producing about 1 kg of seeds for the evaluations. The high molecular weight (HMW) glutenin subunits were analyzed by SDS-PAGE. Statistical correlations were highly significant between HMW glutenin subunits and alveograph and SDS sedimentation. These results indicate the possibility of manipulating major genes for wheat seed quality by coupling traditional breeding with non-destructive single seed analysis. Only half seed is necessary to perform the SDS-PAGE analysis. Therefore, the other half seed can be planted to generate the progeny. Seed yield and SDS sedimentation were statistically correlated, indicating the possibility of simultaneous selection for both traits
面包品质是小麦遗传改良的重要指标之一。虽然在育种计划中进行了大量的质量性状分析,如粉质谱、十二烷基硫酸钠(SDS)沉降、牙槽图和烘烤,但这些分析需要大量的种子,而这些种子只能在后期获得。本实验对谷蛋白高分子量亚基与面包品质的统计相关性进行了评价。在相同的条件下种植了17种小麦基因型,每种基因型生产约1公斤用于评估的种子。SDS-PAGE分析了高分子量谷蛋白亚基。HMW谷蛋白亚基与肺泡沉降和SDS沉降之间具有高度的统计学相关性。这些结果表明,将传统育种与非破坏性单粒分析相结合,可以操纵影响小麦种子品质的主要基因。只需要一半种子就可以进行SDS-PAGE分析。因此,可以种植另一半种子来产生后代。籽粒产量与SDS沉降呈显著的统计学相关性,表明两种性状可能同时被选择
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引用次数: 10
Production of recombinant antigens in plants for animal and human immunization - a review 植物中用于动物和人免疫的重组抗原的制备综述
Pub Date : 1997-12-01 DOI: 10.1590/S0100-84551997000400024
E. Hansen
Plants present a cost effective production system for high value proteins. There is an increasing world demand for cheap vaccines that can be readily administered to the population, especially in economically less developed regions. A promising concept is the production of vaccines in plants that could be grown locally. Expression of antigenic peptides in the palatable parts of plants can lead to the production of edible active vaccines. Two major strategies are: i) to express antigens in transgenic plants, and ii) to produce antigenic peptides on the surface of plant viruses that could be used to infect host plants. This review considers the experimental data and early results for both strategies, and discusses the potential and problems of this new technology
植物提供了一种成本效益高的高价值蛋白质生产系统。世界对廉价疫苗的需求日益增加,这些疫苗可以方便地提供给人口,特别是在经济欠发达地区。一个很有前景的概念是在当地种植的植物中生产疫苗。抗原肽在植物可食用部分的表达可导致可食用活疫苗的生产。两种主要策略是:1)在转基因植物中表达抗原;2)在植物病毒表面产生抗原肽,用于感染寄主植物。本文回顾了这两种策略的实验数据和早期结果,并讨论了这种新技术的潜力和问题
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引用次数: 5
Image analysis and definition of nuclear phenotypes 核表型的图像分析和定义
Pub Date : 1997-12-01 DOI: 10.1590/S0100-84551997000400031
B. Vidal
Some basic topics concerned with the extraction of textural and geometric information from cell nucleus images as well as description and characterization of chromatin supraorganization and consequent classification of nuclear phenotypes are presented.
从细胞核图像中提取纹理和几何信息以及染色质超组织的描述和表征以及随后的核表型分类提出了一些基本主题。
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引用次数: 9
Genetic variability in salt tolerance during germination of Stylosanthes humilis H.B.K. and association between salt tolerance and isozymes 矮茎柱花萌发耐盐遗传变异及其同工酶的关系
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300014
M. B. Lovato, P. Martins
A variacao na tolerância salina na germinacao dentro de seis populacoes naturais de Stylosanthes humilis, provenientes de tres regioes ecogeograficas do Estado de Pernambuco, Mata (clima tropical umido), Agreste e Sertao (clima tropical semiarido), foi determinada submetendo-se sementes para germinar em NaCl 201 mM. Os resultados mostraram diferencas significativas entre familias de todas as populacoes para porcentagem de germinacao e de cinco populacoes para velocidade de germinacao, com excecao da populacao Tamandare (Mata). As populacoes das regioes semiaridas mostraram altos coeficientes de variacao genetica, sendo as do Agreste maiores que as do Sertao. As populacoes da Mata apresentaram baixos coeficientes de variacao genetica. Os coeficientes de determinacao genotipica foram altos para todas as populacoes, com excecao de Tamandare, tanto para porcentagem de germinacao (³ 0,89), como para velocidade de germinacao (³ 0,79), indicando a possibilidade de selecao para tolerância salina na germinacao nessas populacoes. Foram tambem realizadas analises de isoenzimas de esterases e peroxidases e estabelecidas correlacoes entre tolerância salina e frequencias alelicas dessas populacoes. A analise de familias sensiveis e tolerantes ao sal de populacoes do Agreste mostrou uma associacao de alelos de um loco de peroxidase com a tolerância salina durante a germinacao na populacao proveniente de Caruaru
目标在宽容同时在六populacoes germinacao Stylosanthes自然平和,从三个地区ecogeograficas伯南布哥州,杀(疏umido)、矮小和Sertao(疏semiarido),确定提交种子发芽在氯化钠201毫米。结果显示显著差异的家庭所有populacoes之间比例的germinacao和五populacoes germinacao速度,除了塔曼达雷人(马塔)。半干旱地区的种群表现出较高的遗传变异系数,其中野生种群的遗传变异系数大于Sertao种群的遗传变异系数。森林种群的遗传变异系数较低。除大小、发芽率(0.89³)和发芽率(0.79³)外,所有群体的基因型决定系数均较高,表明这些群体的萌发具有耐盐性选择的可能性。对酯酶和过氧化物酶的同工酶进行了分析,并建立了这些人群的盐耐受性和等位基因频率之间的相关性。对野生种群敏感和耐盐科的分析表明,在萌发过程中,过氧化物酶位点的等位基因与耐盐性有关
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引用次数: 12
Estimated frequency of B-chromosomes and population density of Astyanax scabripinnis paranae in a small stream 小流中新疆Astyanax scabripinensis的b染色体频率和种群密度估计
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300004
F. Porto‐Foresti, C. Oliveira, E. L. Maistro, F. Foresti
A study was made of the frequency of B-chromosomes and the population density of Astyanax scabripinnis paranae (Pisces, Characidae, Tetragonopterinae) from three stretches of the Cascatinha stream (Botucatu, SP). In the first stretch the population was estimated to be about 212 individuals and among 35 karyotyped specimens, 23 carried one macro B-chromosome; in the second stretch the population was estimated to be about 650 individuals and among 20 specimens karyotyped, two possessed one macro B-chromosome; in the third stretch the population was estimated to be about 107 individuals and among 10 specimens karyotyped, one carried one macro B-chromosome. A significant difference was observed in the frequency of macro B-chromosomes in females (57%) and males (8.7%) (P = 0.0001). These data suggest that the B-chromosome frequency and the populational density are not directly related. The hypothesis of the existence of some adaptive effect conferred by the B-chromosomes to the specimens from the first stretch of the Cascatinha stream is presented and discussed
对卡斯卡蒂尼亚河(Botucatu, SP)三个河段的Astyanax scabripinnis paranae(双鱼座、鱼科、四爪蝶科)的b染色体频率和种群密度进行了研究。在第一期种群估计有212个个体,35个核型标本中有23个携带一条大b染色体;第二期种群估计有650个个体,20个核型标本中有2个具有一条大b染色体;在第三个种群中,估计有107个个体,在10个核型标本中,有一个携带一条大b染色体。女性(57%)和男性(8.7%)的宏b染色体频率差异有统计学意义(P = 0.0001)。这些数据表明,b染色体频率与种群密度没有直接关系。提出并讨论了b染色体对卡斯卡廷哈河第一支流的标本存在某种适应性作用的假设
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引用次数: 29
Clastogenic activity of integerrimine determined in mouse micronucleus assays 小鼠微核法测定胆碱的致裂活性
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300008
M. C. Gimmler-Luz, B. Erdtmann
The pyrrolizidine alkaloid integerrimine, obtained from Senecio brasiliensis, was tested by acute dosing at two concentrations (18.75 and 37.5 mg/kg), and at different times, to establish its ability to induce micronuclei in mouse erythrocytes. This alkaloid was able to increase the frequency of micronucleated polychromatic erythrocytes in both, bone marrow and peripheral blood erythrocytes
采用两种浓度(18.75 mg/kg和37.5 mg/kg)和不同时间的急性给药方法,对巴西塞内西奥(Senecio brasiliensis)中吡罗里西啶类生物碱integerrimine进行了实验,以确定其在小鼠红细胞中诱导微核的能力。这种生物碱能够增加骨髓和外周血红细胞中微核多染红细胞的频率
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引用次数: 3
Synthesis of stocks and phenotypic effects of dwarf and bantam sex-linked major genes in egg-type chickens 蛋型鸡矮秆和矮秆性连锁主基因的合成及其表型效应
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300003
R. Custódio
Neste trabalho descreve-se a obtencao de seis planteis experimentais, segregantes para os alelos ligados ao sexo, dwarf (dw), bantam (dwB) e normal (dw+), em galinhas para ovos. Comparacoes entre desempenhos entre planteis hemizigoticos para genes maiores no locus dwarf tambem foram efetuadas com relacao a comprimento de canela medida na 16a semana de idade, peso do corpo adulto, producao de ovos e maturidade sexual. Machos heterozigotos (K dw+/ k dwB) foram acasalados com femeas normais (dw+) e com femeas bantam (dwB). As progenies femeas, nos referidos acasalamentos, foram denominadas de D6.a e D6.b, respectivamente. Galos 7/8 Sebright e 1/8 dw-Leghorn heterozigotos (dwB dw) foram acasalados com tres grupos de galinhas de diferentes genotipos (dw+, dw e dwB). As progenies das maes normais (dw+), dwarf (dw) e bantam (dwB) foram denominadas de planteis D4.b, D4.c e D4.d, respectivamente. As seguintes conclusoes foram relatadas: a) a reducao no peso corporal adulto de femeas dwarf, em todos os planteis, em relacao a femeas bantam, foi menor que a relatada em relacao a galinhas normais do tipo ovo; b) a diferenca entre a taxa de postura de galinhas normais e suas irmas bantam nao foi estatisticamente significativa em femeas dw+ (filhas de maes dw+), pesando 1897 g. Porem, em galinhas dwB com peso medio de 1646 g (filhas de maes dwB), a diferenca entre a taxa de postura de galinhas normais e de suas irmas bantam foi significativa (P < 0,05). Portanto, o alelo dwB pode ter um efeito semelhante, porem menor sobre a taxa de postura do que seu alelo dwarf; c) a diferenca entre maturidade sexual de galinhas normais e bantam, filhas de maes maiores ou menores, nao foi estatisticamente significativa, muito embora a postura do primeiro ovo tenha ocorrido 2,9 dias mais tarde em frangas dwB menores; d) o comprimento de canela associado ao peso adulto possibilitou a discriminacao precisa entre bantams e dwarfs.
本研究描述了6个分离性等位基因、矮人等位基因(dw)、矮人等位基因(dwB)和正常等位基因(dw+)的实验容器。比较了16周龄肉桂长度、成体体重、产蛋量和性成熟度等性状,并对矮座大基因半裂肉鸡的性能进行了比较。杂合子雄性(K dw+/ K dwB)与正常雌性(dw+)和矮腿雌性(dwB)交配。在这些交配中,雌性后代被称为D6。a和D6。b分别。7/8 Sebright和1/8 dw-Leghorn杂合子鸡(dwB dw)与3组不同基因型(dw+、dw和dwB)鸡交配。正常母马(dw+)、矮母马(dw)和矮母马(dwB)的后代被称为D4母马。b, D4.c和D4。d分别。报告了以下结论:a)与矮腿雌鸡相比,所有鸡群中矮腿雌鸡的成年体重下降均小于正常蛋型鸡的成年体重下降;b)与正常蛋型鸡相比,所有鸡群中矮腿雌鸡的成年体重下降均小于正常蛋型鸡的成年体重下降b)体重1897 g的dw+母鸡(dw+母鸡的女儿)的正常母鸡和幼仔的产蛋率差异无统计学意义。而在平均体重为1646 g的dwB母鸡(maes dwB的女儿)中,正常母鸡的产蛋率与bantam姐妹母鸡的产蛋率差异显著(P < 0.05)。因此,dwB等位基因对产蛋率的影响可能与dwarf等位基因相似,但影响较小;c)正常母鸡和矮母鸡(大母鸡和小母鸡的女儿)性成熟度的差异没有统计学意义,尽管小母鸡dwB的第一个蛋是在2.9天后下蛋的;d)与成虫体重相关的肉桂长度可以准确区分矮人和矮人。
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引用次数: 1
Chromosome number and secondary constriction variation in 51 accessions of a citrus germplasm bank 柑橘种质库51份材料的染色体数目和次生缩窄变异
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300021
M. Guerra, A. Pedrosa, A. E. B. E. Silva, M. T. M. Cornélio, K. G. B. Santos, W. S. Filho
The mitotic chromosomes of 51 citrus accessions from the Centro Nacional de Pesquisa em Mandioca e Fruticultura Tropical, Cruz das Almas, BA, Brazil, were analyzed. The sample included representatives of 20 Citrus species, one of Poncirus and seven hybrids. All accessions showed 2n = 18 without any evidence of numerical variation. The most clearly variable karyotype feature was the number and position of secondary constrictions (SECs). In 19 accessions the SECs were not identified, mainly due to the degree of chromatin condensation. In the remainder they varied in number from one to three per karyotype. They were found in the proximal region of one of the three largest chromosome pairs, in the terminal/subterminal region of a smaller chromosome or, more seldom, terminally in a larger chromosome. Only in a few cases were such constrictions observed simultaneously in both homologues of the same chromosome pair. The high variability of this karyotype feature may be due to the activation of this region in the previous interphase but may also indicate a high structural variability and heterozygosity of citrus germplasms
对来自巴西鲁兹达斯阿尔马斯省国家热带果树种植中心的51份柑橘有丝分裂染色体进行了分析。样本包括柑橘20个品种的代表,柑桔1个品种和7个杂交种。所有材料均显示2n = 18,无数值变化迹象。最明显的变异核型特征是次级缢痕(SECs)的数量和位置。在19份材料中,主要由于染色质凝结的程度,未鉴定出SECs。在其余的核型中,它们的数量从1到3不等。它们被发现在三个最大的染色体对之一的近端区域,在一个较小的染色体的末端/亚末端区域,或更罕见的,在一个较大的染色体的末端。只有在少数情况下,在同一对染色体的两个同源物中同时观察到这种收缩。这种核型特征的高变异性可能是由于该区域在前间期的激活,但也可能表明柑橘种质的高结构变异性和杂合性
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引用次数: 36
Plant height reduction in populations of triticale (X triticosecale Wittmack) by induced mutations and artificial crosses 诱导突变和人工杂交降低小黑麦群体株高的研究
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300020
Fábio Pandini, F. Carvalho, J. F. B. Neto
Induced mutations by gamma radiation (0, 5, 10, 20 and 40 kR doses) and reciprocal crosses were tested as mechanisms of enhancing genetic variability for plant height in two triticale cultivars, BR4 and EMBRAPA18. The reciprocal crosses and all doses of radiation showed similar increase in genetic amplitude for this trait, being suitable for increasing variability in breeding programs. Genotypes showed different responses as the gamma ray doses were increased, expressing shorter plant height. The decision of using induced mutations or artificial crosses depends on the resources available and the selection method to be used
以2个小黑麦品种BR4和embrapap18为材料,研究了γ辐射(0、5、10、20和40 kR剂量)和互交诱导突变增强株高遗传变异的机制。互反杂交和所有剂量的辐射均显示出该性状遗传幅度的相似增加,适合于增加育种方案中的变异性。随着γ射线剂量的增加,基因型表现出不同的反应,表达出较短的株高。选择诱导突变还是人工杂交取决于可利用的资源和选择方法
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引用次数: 14
Estimates of genetic and phenotypic parameters in a segregant population of rice irrigated by continuous flooding 连续洪水灌溉水稻分离群体遗传和表型参数的估计
Pub Date : 1997-09-01 DOI: 10.1590/S0100-84551997000300013
Patrícia Guimarães Santos, A. Soares, M. Ramalho
Estimates of genetic and phenotypic parameters were obtained by using data from families of a recurrent selection program in rice. An experiment using population CNA-IRAT 4ME/1/1 was conducted at two locations (Lambari and Cambuquira) in the State of Minas Gerais, Brazil. At Lambari, families S0:2 and S0:3 were assessed during crop seasons 1992/1993 and 1993/1994, respectively. In the Cambuquira trial, only S0:3 families were tested in 1993/1994. The experimental design was a 10 x 10 lattice with three replications. The following traits were assessed: grain yield (GY), mean number of days to flowering (FL), plant height (PH), and the incidence of neck blast (NB) caused by Pyricularia grisea and grain staining (GS) caused by Drechslera oryzae. This population proved to be promising for recurrent selection, as it had high average yield and genetic variability. Heritability estimates obtained using variance components were generally greater than estimates of realized heritability, and heritability obtained by parent-offspring regression
遗传和表型参数的估计是通过使用水稻循环选择计划的家庭数据获得的。利用种群CNA-IRAT 4ME/1/1在巴西米纳斯吉拉斯州的两个地点(Lambari和Cambuquira)进行了试验。在Lambari,分别在1992/1993年和1993/1994年作物季节评估了第0:2和第0:3户。在Cambuquira试验中,1993/1994年只有50:3个家庭接受了测试。实验设计为10 × 10格子,重复3次。评价了稻瘟病病的籽粒产量(GY)、平均开花天数(FL)、株高(PH)、稻瘟病颈瘟(NB)和稻瘟病霉(Drechslera oryzae)的籽粒染色(GS)发生率。该群体具有较高的平均产量和遗传变异性,具有良好的循环选择前景。使用方差分量获得的遗传力估计值通常大于实际遗传力估计值,以及通过亲子回归获得的遗传力估计值
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引用次数: 0
期刊
Brazilian Journal of Genetics
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