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Effect of Different Kefir Grain Starter Concentration on Yield, pH, CO2 Content, and Organoleptic Properties of Buffalo Milk Kefir 不同开菲尔颗粒发酵剂浓度对水牛奶开菲尔产量、pH、CO2含量及感官特性的影响
Pub Date : 2020-05-27 DOI: 10.17728/jaft.6513
Lorentia Lydia Margareth, N. Nurwantoro, H. Rizqiati
This research was investigated the effect of different kefir grain starter concentration on yield, pH, carbon dioxide (CO2) content, and organoleptic properties of buffalo milk kefir, and to investigated the ideal kefir grain concentration for good quality of kefir. Complete randomized design used in this research with 4 treatments and 5 replications in concentration 2.5, 5, 7.5, and 10% g/v kefir grain. Buffalo milk, kefir grain, Na2CO3 solution, 1% PP solution, pH meter, burette, and analytical balance were used in this research. The yield and pH of kefir was measured. Carbon dioxide (CO2)  content was measured by Na2CO3 titration, while the organoleptic properties that included level of sourness, sour aroma, texture, and overall acceptance were done by 25 panelists. The results indicated that different kefir grain starter concentration significantly affected to the yield, pH, CO2 content, and organoleptic properties (p<0.05). The most optimal kefir grain concentration was 5% proved by yield 81.81%, pH value 4.20, CO2 content 0.43%, and proper organoleptic properties which were low level of sourness, acceptable aroma of kefir and texture. As conclusion, the concentration of grain relied on the kefir properties that may achieve in the optimum quality of kefir.
本研究研究了不同开菲尔颗粒发酵剂浓度对水牛奶开菲尔产量、pH值、二氧化碳含量和感官特性的影响,并探讨了开菲尔优质的理想开菲尔颗粒浓度。本研究采用完全随机设计,在浓度为2.5、5、7.5和10%g/v的开菲尔颗粒中进行4次处理和5次重复。本研究使用水牛奶、开菲尔谷物、Na2CO3溶液、1%PP溶液、pH计、滴定管和分析天平。测定了开菲尔的产率和pH值。二氧化碳(CO2)含量通过Na2CO3滴定法测量,而包括酸味、酸味、质地和整体接受度在内的感官特性由25名小组成员完成。结果表明,不同的开菲尔颗粒发酵剂浓度对产量、pH值、CO2含量和感官性能有显著影响(p<0.05),最适开菲尔颗粒浓度为5%,产量81.81%,pH值4.20,CO2含量0.43%,具有良好的感官性能,即酸度低、开菲尔香气好、质地好。总之,颗粒的浓度取决于开菲尔的性质,而开菲尔的特性可以实现开菲尔的最佳质量。
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引用次数: 4
Yeast Detection in Mango Flavored Kefir from Small Scale Manufacture in Semarang, Central Java, Indonesia 中爪哇三宝垄小规模生产芒果味开菲尔的酵母检测
Pub Date : 2020-05-24 DOI: 10.17728/jaft.4488
A. N. Al-Baarri, Dian Ari Setianingsih, Y. B. Pramono
This research was done to detect total yeast on small scale kefir industry in Semarang, Central Java, Indonesia. The total plate count method was used to analyze total yeast in mango flavored kefir. This research used mango flavored kefir since this products was the top demand by consumer among kefir flavored products that was produced in this manufacture. Kefir from manufacture was transferred to laboratory using container box to provide buffer in the temperature decrease. As result, total yeast in mango flavored kefir was 1,21±0,06x105 CFU/ml. This total yeast was in the range of the Standard Codex 243-2003 which required a minimum total of 104 CFU/ml. The 1 log unit differences should provide the awareness to manufacture to maintain kefir in low temperature to minimise the multiplication of total yeast resulting the appearance of unwanted flavor. As conclusion, the mango flavored kefir was analyzed in total yeast successfully and in the range of Standard Codex of kefir
本研究是在印度尼西亚中爪哇岛三宝垄的小规模开菲尔工业中检测总酵母。采用菌落总数法对芒果味开菲尔中的总酵母进行了分析。本研究使用了芒果口味的开菲尔,因为在该制造厂生产的开菲尔口味产品中,这种产品是消费者的最大需求。使用容器箱将生产的Kefir转移到实验室,以在温度下降时提供缓冲。结果表明,芒果味开菲尔酵母总量为1,21±0,06x105CFU/ml。该酵母总量在标准法典243-2003的范围内,该标准法典要求最低总量为104 CFU/ml。1个对数单位的差异应使制造商意识到在低温下保持开菲尔,以最大限度地减少总酵母的繁殖,从而出现不想要的风味。结果表明,芒果味开菲尔的酵母总量分析结果符合开菲尔标准法典的要求
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引用次数: 1
Effect of Lesser Yam Flour Addition on Water Content, Texture, Crude Fiber Content, and Reducing Sugar Content of Donut Made from Wheat Flour 添加小山药粉对小麦粉制甜甜圈含水量、质地、粗纤维含量和还原糖含量的影响
Pub Date : 2020-04-06 DOI: 10.17728/jaft.7364
Indri Iriana Putri, B. Dwiloka, V. P. Bintoro
This research aimed to determine the addition of lesser yam flour on water content, texture, crude fiber content, and reducing sugar content of donut made from wheat flour. This research was arranged using completely randomized design with 5 treatments and 4 replications consisted of 5 different lesser yam flour concentrations (0–30%) from total flour that had been used. Water content, texture, and crude fiber content were analyzed using Analysis of Variance (ANOVA) at 5% significance level and reducing sugar content was analyzed using regression equation. The result showed the addition of lesser yam flour can reduce water content. However, it can increase texture, crude fiber content, and reducing sugar content. As conclusion, the concentration of lesser yam flour affected the water content, texture, crude fiber, and reducing sugar in donut.
本研究旨在测定小山药粉的加入对小麦粉制成甜甜圈的含水量、质构、粗纤维含量和还原糖含量的影响。本试验采用完全随机设计,5个处理,4个重复,使用5种不同的山药粉浓度(0 ~ 30%)。水分、质构和粗纤维含量采用5%显著性水平的方差分析(ANOVA),还原糖含量采用回归方程。结果表明,添加少量山药粉可以降低水的含量。然而,它可以增加质地,粗纤维含量和还原糖含量。综上所述,山药粉的浓度对甜甜圈的含水量、质地、粗纤维和还原糖均有影响。
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引用次数: 0
The Functional Properties of Buffalo skin Gelatin Extracted Using Crude Acid Protease from Cow’s Abomasum 牛胃粗酸性蛋白酶提取水牛皮明胶的功能特性
Pub Date : 2019-12-05 DOI: 10.17728/jaft.6464
S. Mulyani, Y. Pranoto, F. Setyabudi, A. M. Legowo, U. Santoso
The study was investigated the functional properties of buffalo skin gelatine. Gelatine was extracted from swamp buffalo skin using crude acid protease from cow’s abomasum (CAPC) in concentration variation 0; 2.5; 5; and 7.5 U/mg. The temperature to hydrolysis included at 28 °C, 37°C and 40°C. The emulsion activity index (EAI), Emulsion stability index (ESI), foaming expansion (FE) and foaming stability (FS) were investigated. The interaction between CAPC concentration and hydrolysis temperature has a significant effect (P <0.05) on the emulsion activity index (EAI), emulsion stability index (ESI), foaming expansion (FE) and foaming stability (FS). The highest EAI was obtained in CAPC concentration of 5 U /mg, hydrolysis temperature of 40°C, which was 12.04 m2/g. The higher concentration of CAPC decreased the ESI. The hydrolysis temperature of 40°C produces higher FE than 28°C and 40°C. The highest FE is obtained at CAPC 5U/mg, 37°C hydrolysis temperature, which is 102.93%. The FS values range from 44.91-55.00%. This value is higher than commercial gelatin (bovine skin gelatin) which is 34.90%. The conclusion of this study is that buffalo skin gelatin with the best functional properties was obtained using CAPC 5 U/mg, the hydrolysis temperature of 40°C.
研究了水牛皮明胶的功能特性。采用牛皱胃粗酸性蛋白酶(CAPC)从浓度变化为0的沼泽水牛皮中提取明胶;2.5;5.和7.5U/mg。水解温度包括28°C、37°C和40°C。考察了乳液活性指数(EAI)、乳液稳定性指数(ESI)、发泡膨胀系数(FE)和发泡稳定性(FS)。CAPC浓度与水解温度的相互作用对乳液活性指数(EAI)、乳液稳定性指数(ESI)、发泡膨胀率(FE)和发泡稳定性(FS)有显著影响(P<0.05)。当CAPC浓度为5U/mg,水解温度为40°C时,EAI最高,为12.04m2/g。CAPC浓度越高,ESI越低。40°C的水解温度产生的FE高于28°C和40°C。在CAPC5U/mg,37°C的水解温度下,获得了最高的FE,为102.93%。FS值在44.91-55.00%之间。该值高于商业明胶(牛皮明胶)的34.90%。本研究的结论是,使用CAPC5U/mg/mg,水解温度为40°C,获得了功能性最好的水牛皮明胶。
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引用次数: 4
Review on Pathogenic Bacteria Listeria monocytogenes, The Detection and The Sequencing Gene Methods Isolated from Meat Products 肉制品中单核细胞增生性李斯特菌的检测及基因测序方法综述
Pub Date : 2019-12-05 DOI: 10.17728/jaft.6460
B. Setiani, Y. B. Pramono, L. Purwitasari
A study was conducted to review on pathogenic bacteria Listeria monocytogenes, the detection and the sequencing gene methods isolated from meat products, compare selected methods that detect the presence of Listeria monocytogenes in selected raw and processed meat products. Results indicate that Listeria monocytogenenes (originally named Bacterium monocytogenes) is a gram-positive, non-sporeforming, highly mobile, rod-type, and facultative anaerobic bacterium species. It can grow under temperatures between -1.5°C to 45°C and at pH range between 4.4 and 9.4, with the optimum pH of 7. Rapid methods (PCR based and VIDAS-LDUO®) detected Listeria monocytogenes faster than the conventional method. It was also gathered that Phenotypic identification and Genotypic identification are two types of confirmation test for Listeria monocytogenes. Listeria monocytogenenes can be found in raw meat and meat product because of environmental contamination, cross contamination or error process.
对致病菌单核细胞增多性李斯特菌、从肉制品中分离的检测和基因测序方法进行了综述,比较了检测所选生加工肉制品中单核细胞增生性李斯特菌的方法。结果表明,单核细胞增多李斯特菌(原名单核细胞增生杆菌)是一种革兰氏阳性、非孢子转化、高流动性、杆状兼性厌氧菌。它可以在-1.5°C至45°C的温度下生长,在4.4至9.4的pH范围内生长,最适pH为7。快速方法(基于PCR和VIDAS-LDUO®)比传统方法更快地检测出单核细胞增多性李斯特菌。表型鉴定和基因型鉴定是单核细胞增多性李斯特菌的两种鉴定方法。由于环境污染、交叉污染或工艺错误,在生肉和肉制品中可发现单核细胞增多性李斯特菌。
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引用次数: 0
Assessment of Probiotic Properties of Lactic Acid Bacteria from Traditional Sourdoughs for Bread-Making in Turkey against Some Gut Conditions 土耳其传统面包酵母中乳酸菌对某些肠道状况的益生菌特性评价
Pub Date : 2019-10-10 DOI: 10.17728/jaft.5662
M. Doğan, I. Tekiner
This study aims to assess the probiotic properties of Lactic Acid Bacteria isolated from the traditional sourdoughs used for bread making in Turkey against some gut conditions. A total number of 29 samples from twelve provinces of Turkey were collected, and screened for the presence of lactic acid bacteria using microbiological methods. The microbiological screening yielded 148 presumptive isolates. Of them, 62.8% were characterized as lactic acid strains by VITEK® MS. Following that, the characterized isolates were subjected to probiotic property testing, including gastric acid resistance, bile resistance and hydrophobic ability. The results showed that 44.1% exceeded gastric pH resistance, 33.3% survived under gastrointestinal system bile salt conditions, and 10.8% exhibited high hydrophobicity ability. In conclusion, our study revealed that only 4.3% (1 Enterococcus faecium, 1 Lactobacillus brevis, 1 Lactobacillus pentosus, and 1 Lactobacillus plantarum) out of 93 lactic acid bacteria isolated from the traditional sourdoughs could meet all probiotic requirements against some gut conditions. 
本研究旨在评估从土耳其用于面包制作的传统酵母中分离的乳酸菌对某些肠道疾病的益生菌特性。从土耳其12个省共收集29份样本,采用微生物学方法筛选乳酸菌的存在。微生物学筛选得到148个推定分离株。其中,62.8%的菌株经VITEK®ms鉴定为乳酸菌株。随后,对鉴定的菌株进行了益生菌特性测试,包括耐胃酸、耐胆汁和疏水能力。结果表明,44.1%超过胃pH耐受性,33.3%在胃肠道胆盐条件下存活,10.8%表现出较高的疏水性。综上所述,我们的研究表明,从传统酵母中分离出的93种乳酸菌中,只有4.3%(1株屎肠球菌、1株短乳杆菌、1株戊酸乳杆菌和1株植物乳杆菌)能够满足部分肠道条件下益生菌的全部需求。
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引用次数: 0
Estimation of Shelf Life Pasta Spice for Dekke Mas Na Niura with the Accelerated Shelf Life Test (ASLT) Method Arrhenius Equation. 用加速保质期试验(ASLT)法估算Dekke Mas Na Niura意大利面香料的保质期Arrhenius方程。
Pub Date : 2019-06-21 DOI: 10.17728/JAFT.5170
Natalia Putri Erva Simbolon, B. Setiani, A. Legowo
Dekke mas na niurais a typical local food of North Sumatra made from carp which does not undergo cooking processes such as frying, steaming, burning, or boiling but is only paraded. This study aimed to estimate the shelf life oDekke mas na niura is a typical local food of North Sumatra made from carp which does not undergo cooking processes such as frying, steaming, burning, or boiling but is only paraded. This study aimed to estimate the shelf life of pasta seasonings using the Arrhenius equation model Accelerated Shelf Life Test (ASLT). This research was conducted at the Laboratory of Food Chemistry and Nutrition, Faculty of Animal Science and Agriculture, Diponegoro University, Semarang. The materials used in making seasonings dekke mas na niura are andaliman, pecan, turmeric, kecombrang / rias, onion, garlic, salt, red chili, lime, kaffir lime, galangal, and peanuts. The method used for making pasta spices is by mixing spices and grinding ingredients until smooth. Seasonings are stored at 25°C, 30°C, 35°C and 40°C for 24 hours of storage. The Analysis is carried out every 6 hours. The parameters tested were Aw value, pH value, and total bacteria (TPC). The results of the test data are then made linear regression with the making of orders 0 and order 1, then from the data, the shelf life calculation is done with the Arrhenius equation. The results showed that the highest Aw, pH, and total bacterial values were at 40°C which were stored for 24 hours. The critical point used in estimating shelf life is total bacterial order 1. Estimation of shelf life based on total bacteria is 5.6 days (25°C); 7.1 days (30°C); 5.6 days (35°C); and 5.2 days (40°C). Storage of pasta ingredients dekke mas na niura optimum at 30°C. Overall, the higher the storage temperature and storage time, the shorter the shelf life.f pasta seasonings using the Arrhenius equation model Accelerated Shelf Life Test (ASLT). This research was conducted at the Laboratory of Food Chemistry and Nutrition, Faculty of Animal Science and Agriculture, Diponegoro University, Semarang. The materials used in making seasonings dekke mas na niura are andaliman, pecan, turmeric, kecombrang / rias, onion, garlic, salt, red chili, lime, kaffir lime, galangal, and peanuts. The method used for making pasta spices is by mixing spices and grinding ingredients until smooth. Seasonings are stored at 25°C, 30°C, 35°C and 40°C for 24 hours of storage. The Analysis is carried out every 6 hours. The parameters tested were Aw value, pH value, and total bacteria (TPC). The results of the test data are then made linear regression with the making of orders 0 and order 1, then from the data, the shelf life calculation is done with the Arrhenius equation. The results showed that the highest Aw, pH, and total bacterial values were at 40°C which were stored for 24 hours. The critical point used in estimating shelf life is total bacterial order 1. Estimation of shelf life based on total bacteria is 5.6 days (25°C);
鲤鱼是北苏门答腊岛的一种典型的当地食物,用鲤鱼制成,不经过油炸、蒸、烧或煮等烹饪过程,只经过游行。本研究的目的是估计鲤鱼的保质期。鲤鱼是北苏门答腊岛的一种典型的当地食物,由鲤鱼制成,不经过油炸、蒸、烧、煮等烹饪过程,只经过腌制。本研究旨在利用阿伦尼乌斯方程模型加速货架期试验(ASLT)估计面食调味料的货架期。这项研究是在三宝垄Diponegoro大学动物科学与农业学院食品化学与营养实验室进行的。制作“烤马那牛油”调味料的材料有安达曼、山核桃、姜黄、香姜、洋葱、大蒜、盐、红辣椒、酸橙、酸橙、高良姜和花生。制作面食香料的方法是将香料混合并将配料研磨至光滑。调味料在25°C、30°C、35°C和40°C下保存24小时。分析每6小时进行一次。检测参数为Aw值、pH值、总菌数(TPC)。然后对试验数据的结果进行0阶和1阶的线性回归,然后根据数据用Arrhenius方程进行保质期的计算。结果表明,在40℃保存24 h时,Aw、pH和细菌总数最高。用于估计保质期的临界点是细菌总阶数1。根据细菌总数估计保质期为5.6天(25°C);7.1天(30℃);5.6天(35℃);5.2天(40°C)。意大利面食原料在30°C的温度下保存最佳。总的来说,储存温度和储存时间越高,保质期越短。使用阿伦尼乌斯方程模型加速货架期试验(ASLT)。这项研究是在三宝垄Diponegoro大学动物科学与农业学院食品化学与营养实验室进行的。制作“烤马那牛油”调味料的材料有安达曼、山核桃、姜黄、香姜、洋葱、大蒜、盐、红辣椒、酸橙、酸橙、高良姜和花生。制作面食香料的方法是将香料混合并将配料研磨至光滑。调味料在25°C、30°C、35°C和40°C下保存24小时。分析每6小时进行一次。检测参数为Aw值、pH值、总菌数(TPC)。然后对试验数据的结果进行0阶和1阶的线性回归,然后根据数据用Arrhenius方程进行保质期的计算。结果表明,在40℃保存24 h时,Aw、pH和细菌总数最高。用于估计保质期的临界点是细菌总阶数1。根据细菌总数估计保质期为5.6天(25°C);7.1天(30℃);5.6天(35℃);5.2天(40°C)。意大利面食原料在30°C的温度下保存最佳。总的来说,储存温度和储存时间越高,保质期越短。
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引用次数: 2
Total Lactic Acid Bacteria, pH Values, Soluble Protein and Antioxidant Activity of Dadih using L. casei and L. plantarum with Different Fermentation Time 不同发酵时间干酪乳杆菌和植物乳杆菌发酵大白菜的乳酸菌总数、pH值、可溶性蛋白及抗氧化活性
Pub Date : 2019-05-19 DOI: 10.17728/jaft.26
Ayu Hapsari
Dadih is a traditional fermented food of West Sumatra, Indonesia that is considered being able to provide health benefits. Fermentation starter used a combination of L. casei and L. plantarum used proper fermentation length is expected to generate optimal antioxidant activity which increases the functional properties of the dadih, it can be classified as a functional food. The purpose of the research was to find out the total lactic acid, pH values, soluble protein, and antioxidant activity using different length of fermentation with a combination starter L. casei and L. plantarum. Complete Random Design with 5 treatments and 4 replicates was used as the experimental design. The treatments given consists of T0 = incubation of dadih for 48 hours, T1 = incubation for 60 hours, T2 = incubation for 72 hours, T3 = incubation during the 84 hours, T4 = incubation for 96 hours. The results showed that long fermentation resulted in the significant changes on the total lactic acid bacteria (LAB), pH values, protein soluble, and antioxidant activity (P<0.05). As conclusion, different length of fermentation increased lactic acid bacteria and total antioxidant activity in dadih. Optimum fermentation in 72 hours length of time, this corresponds in order to produce dadih with antioxidant activity and optimal total lactic acid, pH values and protein soluble.
Dadih是印度尼西亚西苏门答腊的一种传统发酵食品,被认为能够提供健康益处。发酵剂使用干酪乳杆菌和植物乳杆菌的组合,使用适当的发酵长度,有望产生最佳的抗氧化活性,从而提高dadih的功能特性,可以将其归类为功能性食品。本研究的目的是通过干酪乳杆菌和植物乳杆菌的组合发酵,了解不同发酵时间的总乳酸、pH值、可溶性蛋白质和抗氧化活性。采用5个处理和4个重复的完全随机设计作为实验设计。所给予的处理包括T0=dadih孵育48小时,T1=孵育60小时,T2=孵育72小时,T3=在84小时内孵育,T4=孵育96小时。结果表明,长时间发酵可使大麦总乳酸菌数、pH值、蛋白可溶性和抗氧化活性发生显著变化(P<0.05)。最佳发酵时间为72小时,这对应于生产具有抗氧化活性和最佳总乳酸、pH值和蛋白质可溶性的dadih。
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引用次数: 1
Multilevel Extraction for Reducing Tannin of Mangrove Fruit (Bruguiera gumnorrhiza Lamk) as a Raw Material in Mangrove Flour 以红树果实为原料,多级提取还原红树面粉中的单宁
Pub Date : 2019-05-19 DOI: 10.17728/jaft.22
S. Subandriyo
One of the kind of mangrove fruits can be used spesifically as source of food rich of carbohydrate is from Bruguiera gumnorrhiza (tancang) spesies. This kind of mangrove fruit can be explored as an alternative food and can be treated to be flour rich of carbohydrate. Therefore this kind of mangrove can be developed as source of food based on local resources, considering Indonesia is an island nation so that this kind of mangrove can be cultivated along the coast line. Extraction reducing tannin conducted by fixed variable that is comparison between solvent volume and mangrove fruit chips 3:1, while the changed variable are solvent temperature 50,60,70,80oC and time of immersion 15, 30, 45, 60 minute. The optimal result extraction tannin of mangrove fruit obtained at 80oC temperature with time of immersion 60 minutes. The resulting mangrove fruit meets the national standard of consumption flour with content of HCN 0,26 ppm and tannin 4,56 ppm.
作为富含碳水化合物的食物来源,有一种红树林水果可以特别使用,它来自于Bruguiera gumnorrhiza(tanchang)物种。这种红树林水果可以被探索作为一种替代食品,并且可以被处理成富含碳水化合物的面粉。因此,考虑到印度尼西亚是一个岛国,可以在海岸线种植这种红树林,因此可以根据当地资源开发这种红树林作为食物来源。提取还原单宁采用固定变量进行,即溶剂体积与红树林果片3:1的比较,而变化变量为溶剂温度50、60、70、80℃和浸泡时间15、30、45、60分钟。在80℃、浸泡时间60min的条件下提取红树果实单宁的最佳工艺。所得红树果符合国家食用面粉标准,其中HCN含量为0.26ppm,单宁含量为4.56ppm。
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引用次数: 1
Preservation of Snake Fruit Cultivar Pondoh (Salacca edulis Reinw.) at Cold Temperature Storage 蛇果品种蓬多(Salacca edulis Reinw.)的低温保存
Pub Date : 2019-05-02 DOI: 10.17728/JAFT.4369
A. C. D. Wratsongko, A. Legowo, A. N. Al-Baarri, M. Hadipernata, W. Broto
This study was done to preserve snake fruit cultivar Pondoh cold temperature storage. Snake fruits was obtained from Turi, Sleman, Yogyakarta and transferred to the laboratory with the aseptic condition. The sortation based on the visual method was used to collect the proper snake fruit. The cleaning using aseptic cloth was done prior to storage. The snake fruit was collected individually in refrigeration containers at temperatures of 10±5˚C. The storage was done for 30 days and it was stopped until appearance of physical damage in snake fruit. The result indicates that the storage using this method might extend the shelf-life of snake fruit until 28 days. However, snake fruit was hardly to peeled and dry. It can be concluded that cold temperature might extend the shelf-life of snake fruit.
本研究是为了保存蛇果品种Pondoh的低温贮藏。蛇果是从图里,Sleman,Yogyakarta获得的,并在无菌条件下转移到实验室。采用基于视觉方法的分类方法来收集合适的蛇果。使用无菌布清洁是在储存前进行的。蛇果被单独收集在温度为10±5˚C的冷藏容器中。储存30天,并停止储存,直到蛇果出现物理损伤。结果表明,采用该方法贮藏可使蛇果的保质期延长至28天。然而,蛇果很难去皮和干燥。可以得出结论,低温可能延长蛇果的保质期。
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引用次数: 1
期刊
Journal of Applied Food Technology
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