{"title":"Functions of kinesin-1 motor protein in the telotrophic meroistic ovary of red flour beetle, Tribolium castaneum","authors":"Taiki Sunada, E. Kotani, Y. Kaneko, K. Takaki","doi":"10.11416/JIBS.89.1_017","DOIUrl":"https://doi.org/10.11416/JIBS.89.1_017","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.89.1_017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63989495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mai Morishita, H. Mon, J. M. Lee, T. Kusakabe, Maximiano Corrêa Cassal, C. Yasunaga–Aoki, K. Iiyama
{"title":"Toxin complex is a major virulence determinant of Enterobacter sp. 532 against the silkworm, Bombyx mori","authors":"Mai Morishita, H. Mon, J. M. Lee, T. Kusakabe, Maximiano Corrêa Cassal, C. Yasunaga–Aoki, K. Iiyama","doi":"10.11416/JIBS.88.1_021","DOIUrl":"https://doi.org/10.11416/JIBS.88.1_021","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.88.1_021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63989208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenta Tomihara, Katsuya Satta, T. Shimada, T. Kiuchi
{"title":"CRISPR/Cas9-mediated somatic mutation of the sex-linked translucent (os) gene in the silkworm, Bombyx mori","authors":"Kenta Tomihara, Katsuya Satta, T. Shimada, T. Kiuchi","doi":"10.11416/JIBS.88.2_031","DOIUrl":"https://doi.org/10.11416/JIBS.88.2_031","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and characterization of superoxide dismutase in silkworm seminal fluid","authors":"S. Nagaoka, K. Yamamoto","doi":"10.11416/JIBS.88.2_039","DOIUrl":"https://doi.org/10.11416/JIBS.88.2_039","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"88 1","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.88.2_039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroki Yamada, Nao Niimi, M. Itoh, Y. Inoue, Yasuko Kato
{"title":"The Eye-loss mutant, eym is a new allele of eyg, lacking an enhancer required for eye development in Drosophila","authors":"Hiroki Yamada, Nao Niimi, M. Itoh, Y. Inoue, Yasuko Kato","doi":"10.11416/JIBS.88.3_065","DOIUrl":"https://doi.org/10.11416/JIBS.88.3_065","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haruka Nakamura, Y. Hatakeyama, Reina Arai, Yuji Imura, Moe Takahashi, H. Iwano
{"title":"Germination properties of spores on entomopathogenic microsporidia, Trachipleistophora haruka isolated from Spodoptera litura","authors":"Haruka Nakamura, Y. Hatakeyama, Reina Arai, Yuji Imura, Moe Takahashi, H. Iwano","doi":"10.11416/JIBS.88.2_027","DOIUrl":"https://doi.org/10.11416/JIBS.88.2_027","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63989254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), is an agriculturally crucial animal for silk production. Approximately 820 silkworm mutants have been deposited and categorized according to the color of the pupa, skin color, patterns of the larva, and color of the eggs (http://shigen.nig.ac.jp/silkwormbase/top.jsp). The phenotypes available for B. mori have been used in genetic studies. The recent focus in silkworm research has been on using the silkworm as an animal model in drug discovery and development instead of mice or other animals to confirm drug efficacy, studying the function of silkworm proteins, and utilizing it as an expression host for recombinant proteins. However, the cell cycle, including initiation of DNA replication, has not yet been investigated in B. mori. As an initial step toward understanding this process for this species, we attempted to identify factors involved in DNA replication. Replication factor C (RFC) fulfills essential functions in DNA replication and repair of eukaryotic cells (Mossi and Hubscher, 1998). Eukaryotes have five RFC subunits, designated RFC1 to RFC5, and all of them contribute to RFC activity and chromosome replication (Ohta et al., 2002). These subunits belong to the AAA+ protein family (Ohta et al., 2002) and feature seven well-conserved motifs, the RFC boxes II through VIII, which are crucial for the RFC complex formation (Mossi and Hubscher). Previously, we identified a minichromosome maintenance protein in silkworms, which is one of the proteins involved in DNA replication (Yamamoto et al., 2010). In the process of identifying components important for DNA replication in this species, a cDNA encoding RFC, another DNA replication factor, was obtained. In the present study, sequence alignment and the corresponding phylogenetic tree identified the RFC cDNA as a homologue of the B. mori RFC subunit 2 (bmRFC2). Furthermore, the expression pattern of the RNA encoding bmRFC2 was determined by real-time PCR.
家蚕(鳞翅目:家蚕科)是生产蚕丝的重要农业动物。根据蛹的颜色、皮肤颜色、幼虫的图案和卵的颜色，大约有820种家蚕突变体被保存下来并进行分类(http://shigen.nig.ac.jp/silkwormbase/top.jsp)。家蚕的表型已被用于遗传学研究。近年来，家蚕研究的重点是利用家蚕代替小鼠或其他动物作为药物发现和开发的动物模型来证实药物的疗效，研究家蚕蛋白的功能，并利用家蚕作为重组蛋白的表达宿主。然而，包括DNA复制起始在内的细胞周期尚未在家蚕中进行研究。作为了解该物种这一过程的第一步，我们试图确定参与DNA复制的因素。复制因子C (RFC)在真核细胞的DNA复制和修复中发挥重要作用(Mossi和Hubscher, 1998)。真核生物有5个RFC亚基，分别为RFC1到RFC5，它们都参与RFC活性和染色体复制(Ohta et al.， 2002)。这些亚基属于AAA+蛋白家族(Ohta et al.， 2002)，具有7个保守性良好的基序，即RFC盒II至RFC盒VIII，它们对RFC复合物的形成至关重要(Mossi和Hubscher)。此前，我们在家蚕中发现了一种微小染色体维持蛋白，这是参与DNA复制的蛋白质之一(Yamamoto et al.， 2010)。在鉴定该物种DNA复制重要组分的过程中，获得了编码另一种DNA复制因子RFC的cDNA。在本研究中，序列比对和相应的系统发育树鉴定出RFC cDNA为家蚕RFC亚基2 (bmRFC2)的同源物。此外，通过实时PCR检测编码bmRFC2的RNA的表达模式。
{"title":"Identification and expression analysis of the replication factor C protein in the silkworm, Bombyx mori","authors":"K. Yamamoto, M. Haque, F. Saruta","doi":"10.11416/JIBS.88.1_017","DOIUrl":"https://doi.org/10.11416/JIBS.88.1_017","url":null,"abstract":"The silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), is an agriculturally crucial animal for silk production. Approximately 820 silkworm mutants have been deposited and categorized according to the color of the pupa, skin color, patterns of the larva, and color of the eggs (http://shigen.nig.ac.jp/silkwormbase/top.jsp). The phenotypes available for B. mori have been used in genetic studies. The recent focus in silkworm research has been on using the silkworm as an animal model in drug discovery and development instead of mice or other animals to confirm drug efficacy, studying the function of silkworm proteins, and utilizing it as an expression host for recombinant proteins. However, the cell cycle, including initiation of DNA replication, has not yet been investigated in B. mori. As an initial step toward understanding this process for this species, we attempted to identify factors involved in DNA replication. Replication factor C (RFC) fulfills essential functions in DNA replication and repair of eukaryotic cells (Mossi and Hubscher, 1998). Eukaryotes have five RFC subunits, designated RFC1 to RFC5, and all of them contribute to RFC activity and chromosome replication (Ohta et al., 2002). These subunits belong to the AAA+ protein family (Ohta et al., 2002) and feature seven well-conserved motifs, the RFC boxes II through VIII, which are crucial for the RFC complex formation (Mossi and Hubscher). Previously, we identified a minichromosome maintenance protein in silkworms, which is one of the proteins involved in DNA replication (Yamamoto et al., 2010). In the process of identifying components important for DNA replication in this species, a cDNA encoding RFC, another DNA replication factor, was obtained. In the present study, sequence alignment and the corresponding phylogenetic tree identified the RFC cDNA as a homologue of the B. mori RFC subunit 2 (bmRFC2). Furthermore, the expression pattern of the RNA encoding bmRFC2 was determined by real-time PCR.","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"88 1","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.88.1_017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63989150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Fujimoto, A. Okumura, A. Yoshido, Y. Yasukochi, G. Suzuki, K. Sahara
{"title":"Construction of a BAC library and selection of BACs containing orthologs of Bombyx mori genes in Stenopsyche marmorata (Trichoptera: Stenopsychidae)","authors":"T. Fujimoto, A. Okumura, A. Yoshido, Y. Yasukochi, G. Suzuki, K. Sahara","doi":"10.11416/JIBS.87.2_061","DOIUrl":"https://doi.org/10.11416/JIBS.87.2_061","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42891288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Iwamoto, Mervyn Liew Wing On, Lye Ping Ying, S. Noor, K. Takaki, E. Kotani
{"title":"Mass spectrometric profiling of N-linked glycan of recombinant Newcastle disease virus F and HN proteins produced by insect cell expression system","authors":"S. Iwamoto, Mervyn Liew Wing On, Lye Ping Ying, S. Noor, K. Takaki, E. Kotani","doi":"10.11416/JIBS.87.1_001","DOIUrl":"https://doi.org/10.11416/JIBS.87.1_001","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.87.1_001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63989240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The very late promoter-driven ptp transcription can rescue the ELA-defective phenotype of a ptp-disrupted BmNPV","authors":"S. Katsuma","doi":"10.11416/JIBS.87.1_025","DOIUrl":"https://doi.org/10.11416/JIBS.87.1_025","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63989278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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