Abe Shinichi, K. Yamamoto, Y. An, Jun-David Saeki, Itagaki Takashi, H. Kofujita, Koichi Suzuki
{"title":"CO2 anesthesia enhances infection rate of Cordyceps militaris (Hypocreales: Clavicipitaceae) on pupae of the silkworm, Bombyx mori","authors":"Abe Shinichi, K. Yamamoto, Y. An, Jun-David Saeki, Itagaki Takashi, H. Kofujita, Koichi Suzuki","doi":"10.11416/JIBS.83.3_077","DOIUrl":"https://doi.org/10.11416/JIBS.83.3_077","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":"77-81"},"PeriodicalIF":0.0,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.83.3_077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of Residues Essential for Catalytic Activity of a Bombyx mori Arginase","authors":"M. Hossain, S. Nagaoka, K. Yamamoto","doi":"10.11416/JIBS.83.2_047","DOIUrl":"https://doi.org/10.11416/JIBS.83.2_047","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":"47-51"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Etiology of Flacherie, One of the Great Scourges of Sericulture","authors":"T. Hukuhara","doi":"10.11416/JIBS.83.2_025","DOIUrl":"https://doi.org/10.11416/JIBS.83.2_025","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"p143 -mediated rRNA degradation in AcMNPV-infected BM-N cells is not associated with viral DNA replication","authors":"Rina Hamajima, Michihiro Kobayashi, M. Ikeda","doi":"10.11416/JIBS.83.1_019","DOIUrl":"https://doi.org/10.11416/JIBS.83.1_019","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Expression of a BmBR-C Z2/3-isoform encoding a Z2-class protein with an out-of-frame Z3-class specific sequence in the 3'UTR","authors":"Y. Nishita","doi":"10.11416/JIBS.83.1_013","DOIUrl":"https://doi.org/10.11416/JIBS.83.1_013","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Kaneko, K. Kawashita, Hiroki Matsumura, C. Katagiri, N. Ogawa, K. Shirai, Y. Banno
{"title":"Moisture Permeability of Cocoon Shells: Application of Thermogravimetrical method to Small Biological Samples","authors":"F. Kaneko, K. Kawashita, Hiroki Matsumura, C. Katagiri, N. Ogawa, K. Shirai, Y. Banno","doi":"10.11416/JIBS.83.2_041","DOIUrl":"https://doi.org/10.11416/JIBS.83.2_041","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"6 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.83.2_041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Masashi Yuasa, Akitoshi Kitamura, T. Maoka, Takashi Sakudoh, T. Shimada, K. Tsuchida
{"title":"Astaxanthin and lutein compete for accumulation into the middle silk gland via Yellow cocoon gene (C)-dependent control and produce a red cocoon of Bombyx mori","authors":"Masashi Yuasa, Akitoshi Kitamura, T. Maoka, Takashi Sakudoh, T. Shimada, K. Tsuchida","doi":"10.11416/JIBS.83.1_001","DOIUrl":"https://doi.org/10.11416/JIBS.83.1_001","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Sahin Polan, K. Ito, K. Miyamoto, T. Yokoyama, O. Ninagi
{"title":"Linkage analysis of the resistance gene expressed dominantly against the Bt insecticide Cry1Ac in the Silkworm Bombyx mori","authors":"Mohammad Sahin Polan, K. Ito, K. Miyamoto, T. Yokoyama, O. Ninagi","doi":"10.11416/JIBS.83.2_033","DOIUrl":"https://doi.org/10.11416/JIBS.83.2_033","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Yamamoto, H. Ichinose, Y. Aso, Miyako Udono, Y. Katakura
The cytochrome P450 (CYP) superfamily is a highly diversified set of heme-containing proteins that is found in all domains of life, including bacteria, fungi, plants, and animals (Werck-Reichhart and Feyereisen, 2000). CYPs are associated with the catalysis of hydroxylation, dealkylation, and epoxidation (Guengerich, 2001). Through these reactions, CYPs play an important role in the metabolism of xenobiotics, such as pesticides, drugs, pollutants, and naturally occurring toxins, as well as endogenous compounds, including vitamins and steroids (Inouye and Sakaki, 2001; Nebert and Russell, 2002). In insects, CYPs are also involved in the metabolism of hormones and a wide variety of chemicals of natural or synthetic origin, such as plant secondary metabolites and insecticides (Capdevila and Falck, 2001; Capdevila et al., 2002; Kikuta et al., 2002; Oyekan et al., 1998). However, there is little information on the molecular characteristics of CYPs in Lepidoptera, and the relationship between CYPs and insecticide metabolism in the silkmoth has not yet been elucidated. During the course of our studies to identify insecticideinduced mRNAs in the silkmoth, we revealed the mRNA levels of the CYPs that were expressed following the exposure of the silkmoth to insecticides. Previously, we characterized the CYP mRNAs (cyp4M5, cyp6AB4, cyp9A20, and cyp9A22) induced with chemical exposure using microarray experiments (Yamamoto et al., 2010). In this study, we further investigated the different CYPs induced in the silkmoth, Bombyx mori. The expression patterns of CYP mRNAs were examined in response to insecticide treatments by quantitative PCR (qPCR). Many insect pests of agricultural importance are lepidopterans. As the silkmoth is a model animal for lepidopteran insects, it is important to understand its capacity for detoxification, and also the role played by the induced CYPs in the current study. This knowledge can then be applied to other pests.
细胞色素P450 (CYP)超家族是一组高度多样化的含血红素的蛋白质,存在于所有生命领域,包括细菌、真菌、植物和动物(Werck-Reichhart和Feyereisen, 2000)。cyp与羟基化、脱烷基和环氧化的催化作用有关(Guengerich, 2001)。通过这些反应,CYPs在外源物(如农药、药物、污染物和天然毒素)以及内源性化合物(包括维生素和类固醇)的代谢中发挥重要作用(Inouye和Sakaki, 2001;Nebert和Russell, 2002)。在昆虫中,CYPs还参与激素和各种天然或合成来源的化学物质的代谢,例如植物次生代谢物和杀虫剂(Capdevila和Falck, 2001;Capdevila et al., 2002;Kikuta等人,2002;Oyekan et al., 1998)。然而,目前对鳞翅目中CYPs的分子特征了解甚少,且CYPs与家蚕体内杀虫剂代谢的关系尚不清楚。在鉴定杀虫剂诱导的家蚕mRNA的研究过程中,我们揭示了家蚕暴露于杀虫剂后表达的CYPs mRNA水平。此前,我们利用微阵列实验表征了化学暴露诱导的CYP mrna (cyp4M5、cyp6AB4、cyp9A20和cyp9A22) (Yamamoto et al., 2010)。在本研究中,我们进一步研究了在家蚕中诱导的不同CYPs。采用定量PCR (qPCR)检测了CYP mrna在杀虫剂处理下的表达模式。许多重要的农业害虫是鳞翅目。由于蚕蛾是鳞翅目昆虫的模式动物,因此了解其解毒能力以及诱导的CYPs在本研究中所起的作用非常重要。这些知识可以应用于其他害虫。
{"title":"Upregulation of cytochrome p450s following exposure of the silkmoth, Bombyx mori to insecticides","authors":"K. Yamamoto, H. Ichinose, Y. Aso, Miyako Udono, Y. Katakura","doi":"10.11416/JIBS.82.2_033","DOIUrl":"https://doi.org/10.11416/JIBS.82.2_033","url":null,"abstract":"The cytochrome P450 (CYP) superfamily is a highly diversified set of heme-containing proteins that is found in all domains of life, including bacteria, fungi, plants, and animals (Werck-Reichhart and Feyereisen, 2000). CYPs are associated with the catalysis of hydroxylation, dealkylation, and epoxidation (Guengerich, 2001). Through these reactions, CYPs play an important role in the metabolism of xenobiotics, such as pesticides, drugs, pollutants, and naturally occurring toxins, as well as endogenous compounds, including vitamins and steroids (Inouye and Sakaki, 2001; Nebert and Russell, 2002). In insects, CYPs are also involved in the metabolism of hormones and a wide variety of chemicals of natural or synthetic origin, such as plant secondary metabolites and insecticides (Capdevila and Falck, 2001; Capdevila et al., 2002; Kikuta et al., 2002; Oyekan et al., 1998). However, there is little information on the molecular characteristics of CYPs in Lepidoptera, and the relationship between CYPs and insecticide metabolism in the silkmoth has not yet been elucidated. During the course of our studies to identify insecticideinduced mRNAs in the silkmoth, we revealed the mRNA levels of the CYPs that were expressed following the exposure of the silkmoth to insecticides. Previously, we characterized the CYP mRNAs (cyp4M5, cyp6AB4, cyp9A20, and cyp9A22) induced with chemical exposure using microarray experiments (Yamamoto et al., 2010). In this study, we further investigated the different CYPs induced in the silkmoth, Bombyx mori. The expression patterns of CYP mRNAs were examined in response to insecticide treatments by quantitative PCR (qPCR). Many insect pests of agricultural importance are lepidopterans. As the silkmoth is a model animal for lepidopteran insects, it is important to understand its capacity for detoxification, and also the role played by the induced CYPs in the current study. This knowledge can then be applied to other pests.","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"82 1","pages":"33-38"},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63985693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saki Imai, Zhiqing Li, K. Iiyama, Y. Miyagawa, M. Toyoda, A. Umezawa, H. Mon, T. Kusakabe, Kaito Yoshimura, J. M. Lee
{"title":"Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system","authors":"Saki Imai, Zhiqing Li, K. Iiyama, Y. Miyagawa, M. Toyoda, A. Umezawa, H. Mon, T. Kusakabe, Kaito Yoshimura, J. M. Lee","doi":"10.11416/JIBS.82.2_039","DOIUrl":"https://doi.org/10.11416/JIBS.82.2_039","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"82 1","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"2013-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63985816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}