Tomoaki Shigano, Y. Hatakeyama, N. Nishimoto, M. Watanabe, Yuuichi Yamamoto, A. Wijonarko, T. Ohbayashi, H. Iwano
The efficacy of biological control agents has recently been attracting attention because it is now thought to be possible to protect plants from insect pests with minimal effects on the environment. However, many microbial control agents may also affect beneficial insects, such as silkworms and honey bees. For agents to be practical, it is necessary for them not to have an effect on beneficial insects. Microsporidia are obligate parasitic protozoa. Approximately 1,300-1,500 species belonging to 187 genera have been isolated (Vávra and Lukeš, 2013), and most of them infect a variety of insects. It is anticipated that they will be utilized as microbial control agents with selective effects against insect pests because of their host specificity. However, many microsporidia species that infect insect pests of Lepidoptera also infect beneficial insects, such as silkworms. Nosema bombycis is a highly virulent microorganism causing pébrine disease in the silkworm, Bombyx mori, and damage due to this disease has recently been reported worldwide (Hukuhara, 2011). To utilize microsporidia for the control of insect pests, it is necessary to select more host-specific strains with no influence on silkworms. To determine the actual conditions of microsporidian infection in a field population of lepidopteran insects, we conducted a study to isolate and analyze entomopathogenic microsporidia from the common cutworm Spodoptera litura in Chichijima, Ogasawara islands. Chichijima has not been previously explored for microsporidia. We periodically collected male moths of the lepidopteran insect pest S. litura using sex pheromone traps. After detecting microsporidian spores from S. litura moths, we attempted to group the microsporidian strains by spore size comparison and phylogenetic analysis using small-subunit ribosomal RNA (SSU rRNA) sequencing.
生物防治剂的功效最近引起了人们的关注，因为现在认为它可以在对环境影响最小的情况下保护植物免受害虫的侵害。然而，许多微生物控制剂也可能影响益虫，如蚕和蜜蜂。为了使药剂具有实用性，它们必须对益虫不产生影响。小孢子虫是专性寄生原生动物。已分离出187属约1300 - 1500种(Vávra and Lukeš i, 2013)，其中大多数感染多种昆虫。由于其对宿主的特异性，可望作为具有选择性害虫防治作用的微生物防治剂加以利用。然而，许多感染鳞翅目害虫的微孢子虫也感染有益昆虫，如蚕。家蚕微孢子虫是一种高毒力微生物，可引起家蚕(Bombyx mori)的病，最近在世界范围内报道了这种疾病造成的损害(Hukuhara, 2011)。为了利用微孢子虫防治害虫，有必要选择对家蚕无影响的寄主特异性菌株。为了确定鳞翅目昆虫野外种群微孢子虫感染的实际情况，我们对小笠原岛Chichijima常见的斜纹夜蛾(Spodoptera litura)进行了昆虫病原性小孢子虫的分离和分析研究。以前没有在Chichijima研究过微孢子虫。采用性信息素诱捕器定期采集鳞翅目害虫斜纹夜蛾的雄蛾。在检测到斜纹月蛾的微孢子虫孢子后，我们试图通过孢子大小比较和小亚基核糖体RNA (SSU rRNA)测序对微孢子虫菌株进行分类。
{"title":"Variety and diversity of microsporidia isolated from the common cutworm Spodoptera litura in Chichijima, Ogasawara Islands","authors":"Tomoaki Shigano, Y. Hatakeyama, N. Nishimoto, M. Watanabe, Yuuichi Yamamoto, A. Wijonarko, T. Ohbayashi, H. Iwano","doi":"10.11416/JIBS.84.3_069","DOIUrl":"https://doi.org/10.11416/JIBS.84.3_069","url":null,"abstract":"The efficacy of biological control agents has recently been attracting attention because it is now thought to be possible to protect plants from insect pests with minimal effects on the environment. However, many microbial control agents may also affect beneficial insects, such as silkworms and honey bees. For agents to be practical, it is necessary for them not to have an effect on beneficial insects. Microsporidia are obligate parasitic protozoa. Approximately 1,300-1,500 species belonging to 187 genera have been isolated (Vávra and Lukeš, 2013), and most of them infect a variety of insects. It is anticipated that they will be utilized as microbial control agents with selective effects against insect pests because of their host specificity. However, many microsporidia species that infect insect pests of Lepidoptera also infect beneficial insects, such as silkworms. Nosema bombycis is a highly virulent microorganism causing pébrine disease in the silkworm, Bombyx mori, and damage due to this disease has recently been reported worldwide (Hukuhara, 2011). To utilize microsporidia for the control of insect pests, it is necessary to select more host-specific strains with no influence on silkworms. To determine the actual conditions of microsporidian infection in a field population of lepidopteran insects, we conducted a study to isolate and analyze entomopathogenic microsporidia from the common cutworm Spodoptera litura in Chichijima, Ogasawara islands. Chichijima has not been previously explored for microsporidia. We periodically collected male moths of the lepidopteran insect pest S. litura using sex pheromone traps. After detecting microsporidian spores from S. litura moths, we attempted to group the microsporidian strains by spore size comparison and phylogenetic analysis using small-subunit ribosomal RNA (SSU rRNA) sequencing.","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and characterization of a new type of cytosolic sulfotransferase in the silkworm Bombyx mori","authors":"K. Yamamoto, Ming-Cheh Liu","doi":"10.11416/JIBS.84.3_063","DOIUrl":"https://doi.org/10.11416/JIBS.84.3_063","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"63-68"},"PeriodicalIF":0.0,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kaori Yamada, Akinori Yamada, Y. Kawanishi, R. Gurung, Takeshi Sasaki, G. Tokuda, H. Maekawa
Transposable elements (TEs) are present in the genomes of almost all organisms. TEs are classified as either class I or II depending on their mobility mechanism. Class I TEs are retrotransposons that move via a copy– paste mechanism with an RNA intermediate, whereas class II TEs are DNA transposons that move via a cut– paste mechanism as a DNA fragment. Mariner, also referred to as Mos1, is a DNA transposon that was first reported in Drosophila mauritiana (Jacobson et al., 1986; Medhora et al., 1991). It carries a gene encoding a transposase that facilitates self-transposition. Mariner is also present in closely related Drosophila species but is clearly absent from D. melanogaster. A sequence homologous to mariner (Hcmar1) was isolated from the moth Hyalophora cecropia and designated as a mariner-like element (MLE) (Lidholm et al., 1991). At present, MLEs have been isolated from a vast range of phylogenetically distant organisms, such as protozoa (Silva et al., 2005), insects (Robertson, 1993), marine invertebrates (Halaimia-Toumi et al., 2004), and mammals (Auge-Gouillou et al., 1995). According to the current classification, MLEs belong to the Tc1-mariner family, which is a member of the larger IS630-Tc1-mariner superfamily (Shao and Tu, 2001). MLEs can be divided further into five subfamilies: mauritiana, cecropia, mellifera/capitata, irritans, and elegans/briggsae (Robertson and MacLeod, 1993) or into 15 subfamilies (Rouault et al., 2009). The full length of an MLE is approximately 1300 base pairs (bp). This transposon contains one intronless open reading frame (ORF) that encodes a transposase of approximately 340 amino acids, and it is flanked by terminal inverted repeats (TIRs) of approximately 30 bp (Jacobson et al., 1986; Hartl, 1989). MLE transposases that catalyze the transpositional reaction comprise an N-terminal TIR-binding domain with a helix– turn–helix (HTH) motif and a C-terminal catalytic domain with a unique DD(34)D catalytic motif (Shao and Tu, 2001; Plasterk and van Luenen, 2002). In addition, two highly conserved amino acid motifs, WVPHEL and YSPDLAP, are present in the mariner family (Robertson, 1993; Robertson and MacLeod, 1993). In particular, WVPHEL may participate in the formation of the mariner transposase dimer interface (Auge-Gouillou et al., 2005; Liu and Chalmers, 2013). The high sequence similarity of MLEs and their nonuniform distribution, both within genomes and among taxa, strongly suggest that horizontal transfer (HT) has occurred among species (Robertson, 1993; Lohe et al., 1995; Lampe et al., 2003; Rouleux-Bonnin et al., 2005; Casse et al., 2006). The general model of the MLE lifecycle and that of other DNA transposons suggests that a single copy initially invades a genome, which produces multiple copies via duplicative transposition or an unMariner-like elements (MLEs) are DNA transposons that are prevalent in a wide range of eukaryotic genomes and are considered to be inserted into their host genomes via h
转座因子(te)存在于几乎所有生物体的基因组中。根据其流动机制，te可分为第I类或第II类。I类te是逆转录转座子，通过RNA中间体的复制粘贴机制移动，而II类te是DNA转座子，通过剪切粘贴机制作为DNA片段移动。Mariner，也被称为Mos1，是一种DNA转座子，首次在毛里求斯果蝇中被报道(Jacobson et al.， 1986;Medhora et al.， 1991)。它携带一个基因编码转座酶，促进自我转座。水手也存在于密切相关的果蝇物种中，但在D. melanogaster中显然不存在。从盲蛾Hyalophora cecropia中分离到一个与mariner同源的序列(Hcmar1)，并将其命名为mariner-like element (MLE) (Lidholm et al.， 1991)。目前，mle已从广泛的系统发育上遥远的生物中分离出来，如原生动物(Silva等人，2005)、昆虫(Robertson, 1993)、海洋无脊椎动物(Halaimia-Toumi等人，2004)和哺乳动物(Auge-Gouillou等人，1995)。根据目前的分类，mle属于Tc1-mariner家族，它是更大的IS630-Tc1-mariner超家族的成员(Shao and Tu, 2001)。MLEs可进一步分为5个亚科:毛里求斯亚科、cecropia亚科、mellifera/capitata亚科、irritans亚科和elegans/briggsae亚科(Robertson and MacLeod, 1993)或15个亚科(Rouault et al.， 2009)。MLE的全长约为1300个碱基对(bp)。该转座子包含一个编码约340个氨基酸转座酶的无内含子开放阅读框(ORF)，其两侧有约30 bp的末端倒置重复序列(tir) (Jacobson et al.， 1986;哈特尔,1989)。催化转位反应的MLE转座酶包括一个带有螺旋-转螺旋(HTH)基序的n端tir结合域和一个带有独特的DD(34)D催化基序的c端催化域(Shao and Tu, 2001;Plasterk and van Luenen, 2002)。此外，水手家族中存在两个高度保守的氨基酸基序，wvhel和YSPDLAP (Robertson, 1993;罗伯逊和麦克劳德，1993)。特别是，wvhel可能参与mariner转座酶二聚体界面的形成(Auge-Gouillou et al.， 2005;Liu and Chalmers, 2013)。MLEs的高序列相似性及其在基因组内和分类群间的不均匀分布强烈表明物种间发生了水平转移(HT) (Robertson, 1993;Lohe et al.， 1995;Lampe et al.， 2003;Rouleux-Bonnin et al.， 2005;Casse et al.， 2006)。MLE生命周期的一般模型和其他DNA转座子的生命周期模型表明，单个拷贝最初侵入基因组，通过复制转位产生多个拷贝，或者unmariner样元件(MLEs)是DNA转座子，普遍存在于真核生物基因组中，被认为是通过水平转移插入宿主基因组的。本研究对蜘蛛目、膜翅目和鳞翅目3目18种的MLEs进行了系统发育分析，并将其与以往报道的蜜蜂亚科MLEs进行了比较。序列相似性较高(92.78%)。此外，从四个不同的物种获得的mle包含一个完整或几乎完整的开放阅读框，编码一个假定的转座酶。这些mle与其各自宿主物种之间的系统发育差异以及mle的高序列相似性清楚地表明，物种之间的水平转移比以前报道的mle发生了。在分析本研究的基础上，讨论了它们可能的水平转移模式。
{"title":"Widespread distribution and evolutionary patterns of mariner-like elements among various spiders and insects","authors":"Kaori Yamada, Akinori Yamada, Y. Kawanishi, R. Gurung, Takeshi Sasaki, G. Tokuda, H. Maekawa","doi":"10.11416/JIBS.84.2_029","DOIUrl":"https://doi.org/10.11416/JIBS.84.2_029","url":null,"abstract":"Transposable elements (TEs) are present in the genomes of almost all organisms. TEs are classified as either class I or II depending on their mobility mechanism. Class I TEs are retrotransposons that move via a copy– paste mechanism with an RNA intermediate, whereas class II TEs are DNA transposons that move via a cut– paste mechanism as a DNA fragment. Mariner, also referred to as Mos1, is a DNA transposon that was first reported in Drosophila mauritiana (Jacobson et al., 1986; Medhora et al., 1991). It carries a gene encoding a transposase that facilitates self-transposition. Mariner is also present in closely related Drosophila species but is clearly absent from D. melanogaster. A sequence homologous to mariner (Hcmar1) was isolated from the moth Hyalophora cecropia and designated as a mariner-like element (MLE) (Lidholm et al., 1991). At present, MLEs have been isolated from a vast range of phylogenetically distant organisms, such as protozoa (Silva et al., 2005), insects (Robertson, 1993), marine invertebrates (Halaimia-Toumi et al., 2004), and mammals (Auge-Gouillou et al., 1995). According to the current classification, MLEs belong to the Tc1-mariner family, which is a member of the larger IS630-Tc1-mariner superfamily (Shao and Tu, 2001). MLEs can be divided further into five subfamilies: mauritiana, cecropia, mellifera/capitata, irritans, and elegans/briggsae (Robertson and MacLeod, 1993) or into 15 subfamilies (Rouault et al., 2009). The full length of an MLE is approximately 1300 base pairs (bp). This transposon contains one intronless open reading frame (ORF) that encodes a transposase of approximately 340 amino acids, and it is flanked by terminal inverted repeats (TIRs) of approximately 30 bp (Jacobson et al., 1986; Hartl, 1989). MLE transposases that catalyze the transpositional reaction comprise an N-terminal TIR-binding domain with a helix– turn–helix (HTH) motif and a C-terminal catalytic domain with a unique DD(34)D catalytic motif (Shao and Tu, 2001; Plasterk and van Luenen, 2002). In addition, two highly conserved amino acid motifs, WVPHEL and YSPDLAP, are present in the mariner family (Robertson, 1993; Robertson and MacLeod, 1993). In particular, WVPHEL may participate in the formation of the mariner transposase dimer interface (Auge-Gouillou et al., 2005; Liu and Chalmers, 2013). The high sequence similarity of MLEs and their nonuniform distribution, both within genomes and among taxa, strongly suggest that horizontal transfer (HT) has occurred among species (Robertson, 1993; Lohe et al., 1995; Lampe et al., 2003; Rouleux-Bonnin et al., 2005; Casse et al., 2006). The general model of the MLE lifecycle and that of other DNA transposons suggests that a single copy initially invades a genome, which produces multiple copies via duplicative transposition or an unMariner-like elements (MLEs) are DNA transposons that are prevalent in a wide range of eukaryotic genomes and are considered to be inserted into their host genomes via h","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"29-41"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.84.2_029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Mase, Eiji Okada, T. Iizuka, T. Miyajima, Toshio Yamamoto
{"title":"Low Tensile Strength Due to Fragile Points on Silkworm Cocoon Filaments","authors":"K. Mase, Eiji Okada, T. Iizuka, T. Miyajima, Toshio Yamamoto","doi":"10.11416/JIBS.84.2_043","DOIUrl":"https://doi.org/10.11416/JIBS.84.2_043","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.84.2_043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Gotoh, Hideto Nishikawa, K. Sahara, T. Yaginuma, T. Niimi
{"title":"A new molecular technique for determining the sex of Harmonia axyridis","authors":"H. Gotoh, Hideto Nishikawa, K. Sahara, T. Yaginuma, T. Niimi","doi":"10.11416/JIBS.84.1_009","DOIUrl":"https://doi.org/10.11416/JIBS.84.1_009","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kazuyo Enomoto, Y. Hatakeyama, N. Nishimoto, Syouhei Miyake, Hisayuki Oda, Moe Takahashi, Yuuichi Yamamoto, H. Iwano
{"title":"Analysis of taxonomic inference of 40 Bacillus thuringiensis serotypes using genome profiling","authors":"Kazuyo Enomoto, Y. Hatakeyama, N. Nishimoto, Syouhei Miyake, Hisayuki Oda, Moe Takahashi, Yuuichi Yamamoto, H. Iwano","doi":"10.11416/JIBS.84.1_001","DOIUrl":"https://doi.org/10.11416/JIBS.84.1_001","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cultural Attributes and Traditional Knowledge in Connection with the Rearing of Muga (Antheraea assama = assamensis) in the Dhemaji District of Assam, North-East India","authors":"J. Chakravorty, Mallika Gogoi, V. Meyer-Rochow","doi":"10.11416/JIBS.84.1_017","DOIUrl":"https://doi.org/10.11416/JIBS.84.1_017","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"17-28"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation of a Bead-Type Methylated Sericin Drug Delivery Carrier for the Treatment of Helicobacter pylori Infection","authors":"H. Kwak, Yeonwoo Kim, K. Lee","doi":"10.11416/JIBS.83.3_053","DOIUrl":"https://doi.org/10.11416/JIBS.83.3_053","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":"53-58"},"PeriodicalIF":0.0,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.83.3_053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuuichi Yamamoto, Y. Hatakeyama, Kazuyo Enomoto, Tomoaki Shigano, Hisayuki Oda, H. Iwano
{"title":"Isolation and characterization of Bacillus thuringiensis (Bacillaceae: Bacillales) strains from an urban environment","authors":"Yuuichi Yamamoto, Y. Hatakeyama, Kazuyo Enomoto, Tomoaki Shigano, Hisayuki Oda, H. Iwano","doi":"10.11416/JIBS.83.3_071","DOIUrl":"https://doi.org/10.11416/JIBS.83.3_071","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":"71-76"},"PeriodicalIF":0.0,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.83.3_071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete nucleotide sequence of the Eri-silkworm (Samia cynthia ricini) fibroin gene","authors":"H. Sezutsu, K. Yukuhiro","doi":"10.11416/JIBS.83.3_059","DOIUrl":"https://doi.org/10.11416/JIBS.83.3_059","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"83 1","pages":"59-70"},"PeriodicalIF":0.0,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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