A new record of host fish Gymnogobius sp. for glochidia of Pronodularia japanensis , and the availability of host species for the mussel at three paddy field ditches in the Kanto area of Central Japan Abstract This study investigates the availability of host species for glochidia of the unionid mussel Pronodularia japanensis at three paddy field ditches of Kyu-Sasagawa River ( Fujioka City, Gunma Prefecture ) , Okanogouyousui ditch ( Fujioka City, Gunma Prefecture ) , and Nagarakuyousui ditch ( Kawajima Town, Saitama Prefecture ) in the Kanto area in Central Japan. The number of glochidia that infected the fishes, the sites infected, and the glochidial encystment rate were determined using formalin-fixed specimens collected from each ditch. Chestnut gobies, Gymnogobius sp., that were infected with glochidia collected from the Okanogouyousui ditch were cultured in a tank ( 6 L, 24–27 ℃ ) for 10 days to study the appearance of the juvenile detached from the fish body. The major host species of glochidia was different in each study area. At Kyu-Sasagawa River and Okanogouyousui ditch, the glochidia were parasitic on only a few species such as Misgurnus anguillicaudatus and Opsariichthys platypus . At Nagarakuyousui ditch, the glochidia were parasitic on several fish species, such as Op. platypus, Oryzias sp., Rhinogobius sp., and Cyprinus carpio . Of three juveniles detached from the gobies, one survived in a test tube ( 30 mL ) for over 62 days at 21 ℃ . Gymnogobius sp. was identified as a suitable new host for the glochidia of P. japanensis .
{"title":"A new record of host fish Gymnogobius sp. for glochidia of Pronodularia japanensis , and the availability of host species for the mussel at three paddy field ditches in the Kanto area of Central Japan","authors":"T. Itoh, Y. Saitoh, Y. Satoh, Kazuo Ito","doi":"10.3739/RIKUSUI.77.281","DOIUrl":"https://doi.org/10.3739/RIKUSUI.77.281","url":null,"abstract":"A new record of host fish Gymnogobius sp. for glochidia of Pronodularia japanensis , and the availability of host species for the mussel at three paddy field ditches in the Kanto area of Central Japan Abstract This study investigates the availability of host species for glochidia of the unionid mussel Pronodularia japanensis at three paddy field ditches of Kyu-Sasagawa River ( Fujioka City, Gunma Prefecture ) , Okanogouyousui ditch ( Fujioka City, Gunma Prefecture ) , and Nagarakuyousui ditch ( Kawajima Town, Saitama Prefecture ) in the Kanto area in Central Japan. The number of glochidia that infected the fishes, the sites infected, and the glochidial encystment rate were determined using formalin-fixed specimens collected from each ditch. Chestnut gobies, Gymnogobius sp., that were infected with glochidia collected from the Okanogouyousui ditch were cultured in a tank ( 6 L, 24–27 ℃ ) for 10 days to study the appearance of the juvenile detached from the fish body. The major host species of glochidia was different in each study area. At Kyu-Sasagawa River and Okanogouyousui ditch, the glochidia were parasitic on only a few species such as Misgurnus anguillicaudatus and Opsariichthys platypus . At Nagarakuyousui ditch, the glochidia were parasitic on several fish species, such as Op. platypus, Oryzias sp., Rhinogobius sp., and Cyprinus carpio . Of three juveniles detached from the gobies, one survived in a test tube ( 30 mL ) for over 62 days at 21 ℃ . Gymnogobius sp. was identified as a suitable new host for the glochidia of P. japanensis .","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"77 1","pages":"281-291"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69925897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Ueno, K. Takamura, N. Kondo, S. Okuda, Kako Ohbayashi
The family Chironomidae is a very abundant group of benthic macroinvertebrates in freshwater environments. In Japan, 1206 morphospecies of Chironomidae have been recorded. We have determined DNA barcodes for 74 species. Among them, 13 species were excluded from discussion, because they were represented by only a single specimen. In 61 species, the morphospecies matched a single species unit in the phylogenetic tree of partial cytochrome c oxidase subunit I (COI) DNA sequences. However, a morphospecies was divided into two species units in three species and two morphospecies were united into a single species unit. These species are commonly found in ditches, ponds, or lakes, so may bias biodiversity monitoring in these water bodies. We have to redefine these species based on morphological as well as molecular phylogenetic features.
{"title":"Morphospecies does not match DNA barcoding results in a some common Japanese chironomid species","authors":"R. Ueno, K. Takamura, N. Kondo, S. Okuda, Kako Ohbayashi","doi":"10.3739/RIKUSUI.78.27","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.27","url":null,"abstract":"The family Chironomidae is a very abundant group of benthic macroinvertebrates in freshwater environments. In Japan, 1206 morphospecies of Chironomidae have been recorded. We have determined DNA barcodes for 74 species. Among them, 13 species were excluded from discussion, because they were represented by only a single specimen. In 61 species, the morphospecies matched a single species unit in the phylogenetic tree of partial cytochrome c oxidase subunit I (COI) DNA sequences. However, a morphospecies was divided into two species units in three species and two morphospecies were united into a single species unit. These species are commonly found in ditches, ponds, or lakes, so may bias biodiversity monitoring in these water bodies. We have to redefine these species based on morphological as well as molecular phylogenetic features.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3739/RIKUSUI.78.27","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69926744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiocesium from the Fukushima Nuclear Accident that had been deposited on a lake sediment surface was used for an error evaluation of sediment core sampling through disturbance and loss of surface sediments. Sampling was performed in two lakes ( Kasumigaura and Nakanuma lakes ) with different sediment properties using two different methods: the HR-type core sampler and scuba diving. Radiocesium concentrations in the sediment cores changed greatly over the approximate range of depth of 0 to 20 cm, suggesting that radiocesium was usable for an evaluation of sediment sampling. The vertical profiles and inventories of radiocesium taken by the core sampler were similar to those taken by scuba divers, indicating that the disturbance and loss of surface sediments by the core sampler were less pronounced than with collection by divers. These results suggest the successful sampling of undisturbed sediment samples by the HR-type core sampler and the applicability of radiocesium to the assessment of error in sediment core sampling.
{"title":"Assessment of error in sediment core sampling in lakes using radiocesium derived from the Fukushima Nuclear Accident","authors":"Arai Hiroyuki, Fukushima Takehiko, O. Yuichi","doi":"10.3739/RIKUSUI.78.67","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.67","url":null,"abstract":"Radiocesium from the Fukushima Nuclear Accident that had been deposited on a lake sediment surface was used for an error evaluation of sediment core sampling through disturbance and loss of surface sediments. Sampling was performed in two lakes ( Kasumigaura and Nakanuma lakes ) with different sediment properties using two different methods: the HR-type core sampler and scuba diving. Radiocesium concentrations in the sediment cores changed greatly over the approximate range of depth of 0 to 20 cm, suggesting that radiocesium was usable for an evaluation of sediment sampling. The vertical profiles and inventories of radiocesium taken by the core sampler were similar to those taken by scuba divers, indicating that the disturbance and loss of surface sediments by the core sampler were less pronounced than with collection by divers. These results suggest the successful sampling of undisturbed sediment samples by the HR-type core sampler and the applicability of radiocesium to the assessment of error in sediment core sampling.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"67-74"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69926951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic relationships of 33 genera of the subfamily Orthocladiinae in Japan","authors":"K. Kawai, H. Saito, Takuma Anno","doi":"10.3739/RIKUSUI.78.45","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.45","url":null,"abstract":"","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"45-50"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3739/RIKUSUI.78.45","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69926837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The salinity tolerance of freshwater unionid mussels species Inversiunio jokohamensis, Hyriopsis schlegeli, Pronodularia japanensis, Cristaria plicata, C. tenuis , and Sinanodonta sp. reared in tanks was investigated. For adult mussels, the water in the tanks was adjusted to various brackish water salinity levels ( 3, 6, 10, and 12 psu ) . All adult individuals of I. jokohamensis died at salinities above 6 psu. When the salinity was gradually raised from 3-6 to 6-8 psu, almost all adult individuals of I. jokohamensis, H. schlegeli, C. tenuis , and Sinanodonta sp. died, but those of P. japanensis and C. plicata survived. When the salinity was raised from 8 to 10 psu, all adult individuals died. On the other hand, when the salinity levels in tanks were raised from 0-3 to 11-33 psu and lowered from 11-33 to 0 psu until the juveniles fell off from the host ( 186-236 h ) , several glochidia of C. plicata and C. tenuis on the host fish survived and metamorphosed. Thus, the results of the present study show that adult mussels cannot survive in brackish water above salinity levels of 6-8 psu; however, glochidia on a host can survive at high salinities. We also considered the possibility of migration of mussels to other geographical areas via brackish water or the sea.
{"title":"Salinity tolerance of six adults ( Inversiunio jokohamensis , Hyriopsis schlegeli , Pronodularia japanensis , Cristaria plicata , Cristaria tenuis , and Sinanodonta sp.) and two glochidia ( Cristaria plicata and Cristaria tenuis ) of unionid mussels","authors":"T. Itoh, W. Kakino, Tadashi Kitano, H. Kohno","doi":"10.3739/RIKUSUI.78.87","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.87","url":null,"abstract":"The salinity tolerance of freshwater unionid mussels species Inversiunio jokohamensis, Hyriopsis schlegeli, Pronodularia japanensis, Cristaria plicata, C. tenuis , and Sinanodonta sp. reared in tanks was investigated. For adult mussels, the water in the tanks was adjusted to various brackish water salinity levels ( 3, 6, 10, and 12 psu ) . All adult individuals of I. jokohamensis died at salinities above 6 psu. When the salinity was gradually raised from 3-6 to 6-8 psu, almost all adult individuals of I. jokohamensis, H. schlegeli, C. tenuis , and Sinanodonta sp. died, but those of P. japanensis and C. plicata survived. When the salinity was raised from 8 to 10 psu, all adult individuals died. On the other hand, when the salinity levels in tanks were raised from 0-3 to 11-33 psu and lowered from 11-33 to 0 psu until the juveniles fell off from the host ( 186-236 h ) , several glochidia of C. plicata and C. tenuis on the host fish survived and metamorphosed. Thus, the results of the present study show that adult mussels cannot survive in brackish water above salinity levels of 6-8 psu; however, glochidia on a host can survive at high salinities. We also considered the possibility of migration of mussels to other geographical areas via brackish water or the sea.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"87-96"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69927073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sato, Y. Saga, Toshiaki Esumi, Y. Nojiri, Y. Saki, Kenji Kato, Shogo Sugahara, H. Kamiya
COD ( Mn ) is used as an indicator of organic compounds in public waters. Analytical methods for evaluating COD ( Mn ) are simple but entail several problems, such as oxidation rate, and are therefore doubted as appropriate indicators of organic compounds in public waters. In this research, we prepared various aqueous solutions of saccharides and/or amino acids and measured COD and TOC simultaneously. The measured values of TOC were close to the theoretical value for both compounds, and almost all organic carbons in each solution were detectable by TOC equipment. On the other hand, the COD oxidation rate in both the saccharide and amino acid solutions decreased according to the increase in the solutions ’ concentration, and there were also significant differences in the decomposition rate in each compound. The relationship between field data on COD and TOC in Shinji and Nakaumi lakes may be approximated by a quadratic expression, but the approximated curve did not pass the original point. This result indicates that there are some organic compounds that can be decomposed by TOC equipment but not by COD analytical methods, and the decomposition of COD was insufficient in high concentrations in environmental water. Therefore, we consider COD not to be an appropriate indicator of the organic compounds in public waters; instead, TOC should be used.
{"title":"The importance of TOC as an indicator of organic compounds in public waters","authors":"S. Sato, Y. Saga, Toshiaki Esumi, Y. Nojiri, Y. Saki, Kenji Kato, Shogo Sugahara, H. Kamiya","doi":"10.3739/RIKUSUI.78.59","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.59","url":null,"abstract":"COD ( Mn ) is used as an indicator of organic compounds in public waters. Analytical methods for evaluating COD ( Mn ) are simple but entail several problems, such as oxidation rate, and are therefore doubted as appropriate indicators of organic compounds in public waters. In this research, we prepared various aqueous solutions of saccharides and/or amino acids and measured COD and TOC simultaneously. The measured values of TOC were close to the theoretical value for both compounds, and almost all organic carbons in each solution were detectable by TOC equipment. On the other hand, the COD oxidation rate in both the saccharide and amino acid solutions decreased according to the increase in the solutions ’ concentration, and there were also significant differences in the decomposition rate in each compound. The relationship between field data on COD and TOC in Shinji and Nakaumi lakes may be approximated by a quadratic expression, but the approximated curve did not pass the original point. This result indicates that there are some organic compounds that can be decomposed by TOC equipment but not by COD analytical methods, and the decomposition of COD was insufficient in high concentrations in environmental water. Therefore, we consider COD not to be an appropriate indicator of the organic compounds in public waters; instead, TOC should be used.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"59-65"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3739/RIKUSUI.78.59","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69927255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shogo Sugahara, H. Kamiya, Toshiaki Esumi, Y. Tabayashi, Y. Seike
We calculated the changes in the amount of water, total nitrogen ( TN ) , and total phosphorus ( TP ) flowing into Lake Shinji, through a hypothetical flood control channel, using the flow rate data from 1984 to 2013 and L-Q equations developed for the Hii River. Our estimates indicate that in the presence of such a channel, the total water flow into Lake Shinji would be reduced by 1.6%, and TN and TP loads would decrease by 2.8% and 8.9%, respectively. This implies that the concentration of TP has a greater dependence on the flow rate than the concentration of TN. From 2003 to 2014, the ratio of TN to TP at the center of Lake Shinji was 10 to 1. Our results suggest that the operation of a flood control channel would increase this ratio and may cause diatoms to overtake the blue-green algae as the dominant algae in Lake Shinji.
{"title":"Reduction of nutrient loads to Lake Shinji by flood control channel","authors":"Shogo Sugahara, H. Kamiya, Toshiaki Esumi, Y. Tabayashi, Y. Seike","doi":"10.3739/RIKUSUI.77.315","DOIUrl":"https://doi.org/10.3739/RIKUSUI.77.315","url":null,"abstract":"We calculated the changes in the amount of water, total nitrogen ( TN ) , and total phosphorus ( TP ) flowing into Lake Shinji, through a hypothetical flood control channel, using the flow rate data from 1984 to 2013 and L-Q equations developed for the Hii River. Our estimates indicate that in the presence of such a channel, the total water flow into Lake Shinji would be reduced by 1.6%, and TN and TP loads would decrease by 2.8% and 8.9%, respectively. This implies that the concentration of TP has a greater dependence on the flow rate than the concentration of TN. From 2003 to 2014, the ratio of TN to TP at the center of Lake Shinji was 10 to 1. Our results suggest that the operation of a flood control channel would increase this ratio and may cause diatoms to overtake the blue-green algae as the dominant algae in Lake Shinji.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"77 1","pages":"315-320"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69926297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kondo, S. Okuda, Kako Ohbayashi, R. Ueno, K. Takamura
The insect group Chironomidae is used as an environmental indicator for freshwater ecosystems because of its wide distribution and species richness. The morphological identification of chironomids requires expert skill; to this end, the application of DNA barcoding for species identification is a powerful tool. We applied DNA barcoding to chironomid specimens using different preservative conditions and several protocols for extraction and sequencing of DNA and found that the success of sequencing was affected by the condition of specimens and protocols applied. Purification using a silica-membrane filter yielded significantly high DNA quality and was deemed suitable for both old and valuable specimens. On the other hand, rough extraction without any purification and extraction without membrane filters both achieved sufficient DNA quality and sequencing success rates to be applied to wellpreserved and large specimens. For old or air-dried samples, however, rough extraction requires great care because it significantly reduces the efficiency of sequencing. DNA extracted from old or poorly preserved specimens tended to be fragmented and was not successfully sequenced, but using alternative PCR primers targeting a shorter region can improve the success rate. Dried wing specimens on prepared slides and pupal exuviae collected from the water surface were also subjected to DNA extraction and subsequent sequencing, which achieved 18.0% and 41.7% success rates, respectively. Such success rates may be insufficient for some purposes of DNA barcoding, but match particular objectives, for example, a field study with less work by collecting exuviae.
{"title":"Comparison of DNA extraction methods for DNA barcoding of non-biting midges","authors":"N. Kondo, S. Okuda, Kako Ohbayashi, R. Ueno, K. Takamura","doi":"10.3739/RIKUSUI.78.13","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.13","url":null,"abstract":"The insect group Chironomidae is used as an environmental indicator for freshwater ecosystems because of its wide distribution and species richness. The morphological identification of chironomids requires expert skill; to this end, the application of DNA barcoding for species identification is a powerful tool. We applied DNA barcoding to chironomid specimens using different preservative conditions and several protocols for extraction and sequencing of DNA and found that the success of sequencing was affected by the condition of specimens and protocols applied. Purification using a silica-membrane filter yielded significantly high DNA quality and was deemed suitable for both old and valuable specimens. On the other hand, rough extraction without any purification and extraction without membrane filters both achieved sufficient DNA quality and sequencing success rates to be applied to wellpreserved and large specimens. For old or air-dried samples, however, rough extraction requires great care because it significantly reduces the efficiency of sequencing. DNA extracted from old or poorly preserved specimens tended to be fragmented and was not successfully sequenced, but using alternative PCR primers targeting a shorter region can improve the success rate. Dried wing specimens on prepared slides and pupal exuviae collected from the water surface were also subjected to DNA extraction and subsequent sequencing, which achieved 18.0% and 41.7% success rates, respectively. Such success rates may be insufficient for some purposes of DNA barcoding, but match particular objectives, for example, a field study with less work by collecting exuviae.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"13-26"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69926672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DNA-sequence-based species delimitation methods were applied to larval chironomid samples collected from 20 Japanese farm ponds. This is the first trial using DNA barcoding to reveal the species composition of chironomid communites in limnological waters. A total of 72 species were delimited from mitochondria DNA cytochrome c oxidase I sequences of the samples. Of these, the number of species delimited from the total sample, bottom sediment sample, and aquatic weed sample of each pond, were 0-21, 0-15 and 3-16, respectively. Greater numbers of collected larvae resulted in more delimited species. Scientific names were assigned for 31 species.
{"title":"DNA-sequence-based species delimitation for larval chironomid samples collected from Japanese farm ponds","authors":"K. Takamura, R. Ueno, N. Kondo, Kako Ohbayashi","doi":"10.3739/RIKUSUI.78.35","DOIUrl":"https://doi.org/10.3739/RIKUSUI.78.35","url":null,"abstract":"DNA-sequence-based species delimitation methods were applied to larval chironomid samples collected from 20 Japanese farm ponds. This is the first trial using DNA barcoding to reveal the species composition of chironomid communites in limnological waters. A total of 72 species were delimited from mitochondria DNA cytochrome c oxidase I sequences of the samples. Of these, the number of species delimited from the total sample, bottom sediment sample, and aquatic weed sample of each pond, were 0-21, 0-15 and 3-16, respectively. Greater numbers of collected larvae resulted in more delimited species. Scientific names were assigned for 31 species.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"78 1","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3739/RIKUSUI.78.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69926774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inland water fishes in the Ryukyu Islands: intriguing questions","authors":"M. Nishida","doi":"10.3739/RIKUSUI.77.211","DOIUrl":"https://doi.org/10.3739/RIKUSUI.77.211","url":null,"abstract":"","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":"77 1","pages":"211-216"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69925395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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