Pub Date : 2024-02-27DOI: 10.55262/fabadeczacilik.1411353
Ege Arzuk, Fuat Karakuş, Gökay Albayrak, Ali Ergüç, İclal Tan, Ecrin Atiş
Inflammation is an initial biological process that involves the activation of the immune system in response to injury, infection or exposure to toxic agents. During this process, cytokines, small proteins produced by immune cells, play a vital role in regulating the immune response. Inflammatory cytokines, including interleukins, tumor necrosis factor-α, nitric oxide, and interferon-gamma, initiate the immune response and promote inflammation. Natural products are frequently a source of potential anti-inflammatory compounds, and screening natural products can lead to the discovery of novel bioactive compounds. The present study aimed to investigate the effects of n-hexane extract from Tanacetum argenteum subsp. argenteum on the lipopolysaccharide-induced inflammatory response in human macrophages THP-1 cell. Cells were incubated with different concentrations of n-hexane extract, and the inhibitor effects of the extract exposure on various cytokine secretions were determined. The findings demonstrated that n-hexane extract dramatically decreased the levels of interleukin‑6, interleukin‑1β, and tumor necrosis factor‑α in differentiated THP-1 cells, indicating the remarkable anti-inflammatory potential of the extract. Furthermore, in silico analysis of toxicogenomic data revealed the interactions between the active component of the n-hexane extract and interleukin-6, interleukin-1β, and tumor necrosis factor.
{"title":"Effect of n-Hexane Extract from Tanacetum argenteum (Lam.) Willd. subsp. argenteum on the Secretion of Proinflammatory Cytokines in THP-1 Cell Line","authors":"Ege Arzuk, Fuat Karakuş, Gökay Albayrak, Ali Ergüç, İclal Tan, Ecrin Atiş","doi":"10.55262/fabadeczacilik.1411353","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1411353","url":null,"abstract":"Inflammation is an initial biological process that involves the activation of the immune system in response to injury, infection or exposure to toxic agents. During this process, cytokines, small proteins produced by immune cells, play a vital role in regulating the immune response. Inflammatory cytokines, including interleukins, tumor necrosis factor-α, nitric oxide, and interferon-gamma, initiate the immune response and promote inflammation. Natural products are frequently a source of potential anti-inflammatory compounds, and screening natural products can lead to the discovery of novel bioactive compounds. The present study aimed to investigate the effects of n-hexane extract from Tanacetum argenteum subsp. argenteum on the lipopolysaccharide-induced inflammatory response in human macrophages THP-1 cell. Cells were incubated with different concentrations of n-hexane extract, and the inhibitor effects of the extract exposure on various cytokine secretions were determined. The findings demonstrated that n-hexane extract dramatically decreased the levels of interleukin‑6, interleukin‑1β, and tumor necrosis factor‑α in differentiated THP-1 cells, indicating the remarkable anti-inflammatory potential of the extract. Furthermore, in silico analysis of toxicogenomic data revealed the interactions between the active component of the n-hexane extract and interleukin-6, interleukin-1β, and tumor necrosis factor.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140425057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-26DOI: 10.55262/fabadeczacilik.1429111
Melike Hacer Özkan
The effect of the gut microbiota metabolite trimethylamine-(TMA) in isolated vessels is unknown yet. Previously TMAO, the hepatic oxidation product of TMA, at 3 mM has been shown to inhibit endothelium-dependent vasorelaxations of isolated arteries only after 24-hour-interactions. In this study, the effects of TMA (at 1 mM) on endothelium-dependent relaxations with acute (1 or 4 hours) and longer (24 hours) incubation periods were evaluated in superior mesenteric arteries of rat. Acute exposure to TMA of 1 hour significantly inhibited acetylcholine-stimulated endothelium-derived hyperpolarizing (EDH) type relaxations, and this inhibition gradually intensified as the incubation period was prolonged to 4, and 24 hours. The area under the curves (AUCs) of the relaxation-response curves after 1 and 24 hours of TMA incubation were found significantly different compared to each other, whereas similar AUC values were obtained after 4, and 24 hours of incubations. Contractile responses to phenylephrine, and nitric oxide (NO)-mediated relaxations of acetylcholine were similar in arteries before and after pretreatment with TMA for 24 hours. These data indicate that TMA selectively inhibits EDH-type relaxations in rat isolated mesenteric arteries. Although the inhibitory effect of TMA intensifies over time, it appears to be more pronounced during acute incubation periods. The findings strengthen the evidence that TMA is a more toxic metabolite on vascular tone than TMAO.
{"title":"The Inhibitory Effect of Trimethylamine (TMA), an Intestinal Bacterial Metabolite, on Endothelial Vasorelaxation in Rat Mesenteric Artery","authors":"Melike Hacer Özkan","doi":"10.55262/fabadeczacilik.1429111","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1429111","url":null,"abstract":"The effect of the gut microbiota metabolite trimethylamine-(TMA) in isolated vessels is unknown yet. Previously TMAO, the hepatic oxidation product of TMA, at 3 mM has been shown to inhibit endothelium-dependent vasorelaxations of isolated arteries only after 24-hour-interactions. In this study, the effects of TMA (at 1 mM) on endothelium-dependent relaxations with acute (1 or 4 hours) and longer (24 hours) incubation periods were evaluated in superior mesenteric arteries of rat. Acute exposure to TMA of 1 hour significantly inhibited acetylcholine-stimulated endothelium-derived hyperpolarizing (EDH) type relaxations, and this inhibition gradually intensified as the incubation period was prolonged to 4, and 24 hours. The area under the curves (AUCs) of the relaxation-response curves after 1 and 24 hours of TMA incubation were found significantly different compared to each other, whereas similar AUC values were obtained after 4, and 24 hours of incubations. Contractile responses to phenylephrine, and nitric oxide (NO)-mediated relaxations of acetylcholine were similar in arteries before and after pretreatment with TMA for 24 hours. These data indicate that TMA selectively inhibits EDH-type relaxations in rat isolated mesenteric arteries. Although the inhibitory effect of TMA intensifies over time, it appears to be more pronounced during acute incubation periods. The findings strengthen the evidence that TMA is a more toxic metabolite on vascular tone than TMAO.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140428299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.55262/fabadeczacilik.1394893
Mustafa Özyeşil, Havane Tembelo
This study examines the effects of the COVID-19 pandemic on the financial performance of the Turkish pharmaceutical industry in the period between 2018 and 2022. Financial indicators in the sector were examined and analyzed through the data set covering the periods before, during and after the pandemic. The hypotheses on which the research focuses predict the impact of the pandemic process on the pharmaceutical industry. The ADF unit root tests focused on four different financial indicators in the sector and it was determined that these indicators were stationary at normal levels. Hausman test results showed that a fixed-effect model is the most suitable option for the panel data model. However, upon detecting autocorrelation and heteroscedasticity problems in the model, the Panel GLS model was applied. Structural break analysis revealed that there were unexpected changes in the periods determined as the second quarter of 2020 and the first quarter of 2022. These periods mark time periods when the effects of the COVID-19 pandemic are particularly evident. Additionally, new recorded case data of COVID-19 shows how the pandemic's impact on the industry has changed over time. It was observed that pandemic conditions became evident in April and May 2020, and February 2022 marked the highest number of cases. This study aims to evaluate the future potential of the sector by examining in detail the changes in the financial performance of the Turkish pharmaceutical industry before, during and after the pandemic.
{"title":"Financial Development of the Turkish Pharmaceutical Sector During and After the Pandemic: Sector Panel Data Analysis for the Period 2018-2022","authors":"Mustafa Özyeşil, Havane Tembelo","doi":"10.55262/fabadeczacilik.1394893","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1394893","url":null,"abstract":"This study examines the effects of the COVID-19 pandemic on the financial performance of the Turkish pharmaceutical industry in the period between 2018 and 2022. Financial indicators in the sector were examined and analyzed through the data set covering the periods before, during and after the pandemic. The hypotheses on which the research focuses predict the impact of the pandemic process on the pharmaceutical industry. The ADF unit root tests focused on four different financial indicators in the sector and it was determined that these indicators were stationary at normal levels. Hausman test results showed that a fixed-effect model is the most suitable option for the panel data model. However, upon detecting autocorrelation and heteroscedasticity problems in the model, the Panel GLS model was applied. Structural break analysis revealed that there were unexpected changes in the periods determined as the second quarter of 2020 and the first quarter of 2022. These periods mark time periods when the effects of the COVID-19 pandemic are particularly evident. Additionally, new recorded case data of COVID-19 shows how the pandemic's impact on the industry has changed over time. It was observed that pandemic conditions became evident in April and May 2020, and February 2022 marked the highest number of cases. This study aims to evaluate the future potential of the sector by examining in detail the changes in the financial performance of the Turkish pharmaceutical industry before, during and after the pandemic.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140459955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.55262/fabadeczacilik.1391109
Kübra Şener, Murat Eki̇ci̇, Ekrem Murat Gönülalan, Ebru Bodur
Bu çalışmada Lamiaceae familyasından seçilmiş bitkilerin (Mentha piperita L., Salvia officinalis L., Lavandula officinalis Mill., Scutellaria orientalis L. ve Melissa officinalis L.) inflamasyon yolağında önemli bir yeri olduğu bilinen lipoksijenaz-12/15 (LOX-12/15), siklooksijenaz-2 (COX-2) ve asetilkolinesteraz (AChE) aktiviteleri üzerine etkileri araştırılmıştır. İnflamasyon sürecinde yan etkileri fazla bulunan inhibitör ilaçlara alternatif olmaları açısından bu bitki ektstelerinin etkinlikleri incelenmiştir. Ekstrelerin metabolomik profilleri GC-MS ve LC-qTOF-MS yöntemleriyle tanımlanmıştır. Ekstrelerin antioksidan potansiyelleri, DPPH+ radikal süpürücü etki ve TAC yöntemleriyle araştırılmıştır. DPPH+ radikalinin zamana bağlı süpürücü etkileri incelendiğinde, ekstrelerin zamana ve konsantrasyona bağlı olarak etkilerinin değiştiği bulunmuştur. Ekstrelerin 12-15-LOX, COX-2 ve AChE enzimleri üzerindeki potansiyel inhibitör etkileri incelenmiş ve elde edilen sonuçların metabolomik profilleri korelasyon analizi ile karşılaştırılmıştır. Elde edilen sonuçlar, ekstrelerin potansiyel anti-inflamatuar ajanlar olarak kullanıma sahip olduğunu düşündürmektedir.
{"title":"Comparative Study of The Anti-Inflammatory Pathway Enzyme Activities of Selected Plant Extracts from Lamiaceae Family","authors":"Kübra Şener, Murat Eki̇ci̇, Ekrem Murat Gönülalan, Ebru Bodur","doi":"10.55262/fabadeczacilik.1391109","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1391109","url":null,"abstract":"Bu çalışmada Lamiaceae familyasından seçilmiş bitkilerin (Mentha piperita L., Salvia officinalis L., Lavandula officinalis Mill., Scutellaria orientalis L. ve Melissa officinalis L.) inflamasyon yolağında önemli bir yeri olduğu bilinen lipoksijenaz-12/15 (LOX-12/15), siklooksijenaz-2 (COX-2) ve asetilkolinesteraz (AChE) aktiviteleri üzerine etkileri araştırılmıştır. İnflamasyon sürecinde yan etkileri fazla bulunan inhibitör ilaçlara alternatif olmaları açısından bu bitki ektstelerinin etkinlikleri incelenmiştir. Ekstrelerin metabolomik profilleri GC-MS ve LC-qTOF-MS yöntemleriyle tanımlanmıştır. Ekstrelerin antioksidan potansiyelleri, DPPH+ radikal süpürücü etki ve TAC yöntemleriyle araştırılmıştır. DPPH+ radikalinin zamana bağlı süpürücü etkileri incelendiğinde, ekstrelerin zamana ve konsantrasyona bağlı olarak etkilerinin değiştiği bulunmuştur. Ekstrelerin 12-15-LOX, COX-2 ve AChE enzimleri üzerindeki potansiyel inhibitör etkileri incelenmiş ve elde edilen sonuçların metabolomik profilleri korelasyon analizi ile karşılaştırılmıştır. Elde edilen sonuçlar, ekstrelerin potansiyel anti-inflamatuar ajanlar olarak kullanıma sahip olduğunu düşündürmektedir.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140467968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-29DOI: 10.55262/fabadeczacilik.1360277
İ. İyİgÜndoĞdu, I. Cok
Accumulating scientific evidence shows that thyroid hormone synthesis and signaling are now recognized as one of the important targets of environmental chemicals, especially chemicals called endocrine disruptors. Endocrine disrupting chemicals (EDCs) are man-made chemicals and consist of different types of molecules, for instance some pesticides, plasticizers, flame retardants (FRs), surfactants, many of which can interfere with thyroid hormone synthesis or their actions. FRs, important members of endocrine disruptors, share similarities in their chemical structures when compared with thyroid hormones, and there is accumulating scientific findings pointing out that they may take part in the dysfunction of thyroid hormone homeostasis. The primary aim of using FRs is to minimize the risk of fire and prevention of its spreading. The potential effects of exposure to FRs on the thyroid and thyroid hormones have gained importance since they may easily migrate into the surrounding environment and are especially found in house dust. Within the framework of the results of some experimental animal and in vitro studies, as well as limited human studies researching the consequences of FRs on the thyroid system, this paper aims to make a general assessment of whether these chemicals have a role in some thyroid diseases. Although the information that FRs with endocrine disrupting properties may have an effect on thyroid hormone levels and cause disruption in the thyroid system is still in its infancy, there is emerging evidence that some members of FRs may have thyroid disrupting properties.
{"title":"Endocrine Disrupting Effects of Flame Retardants on Thyroid System","authors":"İ. İyİgÜndoĞdu, I. Cok","doi":"10.55262/fabadeczacilik.1360277","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1360277","url":null,"abstract":"Accumulating scientific evidence shows that thyroid hormone synthesis and signaling are now recognized as one of the important targets of environmental chemicals, especially chemicals called endocrine disruptors. Endocrine disrupting chemicals (EDCs) are man-made chemicals and consist of different types of molecules, for instance some pesticides, plasticizers, flame retardants (FRs), surfactants, many of which can interfere with thyroid hormone synthesis or their actions. FRs, important members of endocrine disruptors, share similarities in their chemical structures when compared with thyroid hormones, and there is accumulating scientific findings pointing out that they may take part in the dysfunction of thyroid hormone homeostasis. The primary aim of using FRs is to minimize the risk of fire and prevention of its spreading. The potential effects of exposure to FRs on the thyroid and thyroid hormones have gained importance since they may easily migrate into the surrounding environment and are especially found in house dust. Within the framework of the results of some experimental animal and in vitro studies, as well as limited human studies researching the consequences of FRs on the thyroid system, this paper aims to make a general assessment of whether these chemicals have a role in some thyroid diseases. Although the information that FRs with endocrine disrupting properties may have an effect on thyroid hormone levels and cause disruption in the thyroid system is still in its infancy, there is emerging evidence that some members of FRs may have thyroid disrupting properties.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140488373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-29DOI: 10.55262/fabadeczacilik.1361591
M. S. İçen, H. Yüce, Neşe Başak Türkmen, A. Taslidere, Dilan AŞKIN ÖZEK, S. Ünüvar
Myocardial infarction (MI) is one of the leading causes of death worldwide. In this study aimed to investigate the protective effects of Pistacia palaestina Boiss (PP) leaf and fruit extracts, which are thought to have antioxidant and anti-inflammatory properties, in isoproterenol (ISO)-induced MI. 80 Spraque-Dawley rats were divided into 10 groups. The control group was given saline. PP leaf and fruit extracts at doses of 250 mg/kg and 500 mg/kg applied by gavage for 21 days. ISO 100 mg/kg subcutaneously was administered to the MI and MI-treatment groups on the 17th and 18th days of the experiment. Thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured in heart tissue. In serum, Troponin t, CK-MB; pro-inflammatory cytokine necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β) and IL-6; Anti-inflammatory IL-10 levels were determined by the ELISA method. Heart tissue was examined by haematoxylin-eosin staining. While lipid peroxidation indicator TBARS activity increased in the MI group, antioxidant enzyme activities and GSH levels decreased. While Troponin t, CK-MB, TNF-α, IL-1β and IL-6 levels increased, anti-inflammatory IL-10 levels decreased. Low and high dose PP leaf and fruit extracts significantly decreased TBARS, Troponin t, CK-MB, TNF-α, IL-1β, IL-6 levels, improved antioxidant enzyme activity, GSH and IL-10 levels. PP ameliorated cardiac biomarkers and histopathological changes in ISO-induced MI by suppressing oxidative stress and inflammation. PP extracts may play an important cardioprotective role in the treatment of MI with their antioxidant and anti-inflammatory effects.
{"title":"Protective Role of Pistacia Palaestina Boiss Fruit and Leaf Extracts in Isoproterenol-Induced Cardiac Ischemia","authors":"M. S. İçen, H. Yüce, Neşe Başak Türkmen, A. Taslidere, Dilan AŞKIN ÖZEK, S. Ünüvar","doi":"10.55262/fabadeczacilik.1361591","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1361591","url":null,"abstract":"Myocardial infarction (MI) is one of the leading causes of death worldwide. In this study aimed to investigate the protective effects of Pistacia palaestina Boiss (PP) leaf and fruit extracts, which are thought to have antioxidant and anti-inflammatory properties, in isoproterenol (ISO)-induced MI. 80 Spraque-Dawley rats were divided into 10 groups. The control group was given saline. PP leaf and fruit extracts at doses of 250 mg/kg and 500 mg/kg applied by gavage for 21 days. ISO 100 mg/kg subcutaneously was administered to the MI and MI-treatment groups on the 17th and 18th days of the experiment. Thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured in heart tissue. In serum, Troponin t, CK-MB; pro-inflammatory cytokine necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β) and IL-6; Anti-inflammatory IL-10 levels were determined by the ELISA method. Heart tissue was examined by haematoxylin-eosin staining. While lipid peroxidation indicator TBARS activity increased in the MI group, antioxidant enzyme activities and GSH levels decreased. While Troponin t, CK-MB, TNF-α, IL-1β and IL-6 levels increased, anti-inflammatory IL-10 levels decreased. Low and high dose PP leaf and fruit extracts significantly decreased TBARS, Troponin t, CK-MB, TNF-α, IL-1β, IL-6 levels, improved antioxidant enzyme activity, GSH and IL-10 levels. PP ameliorated cardiac biomarkers and histopathological changes in ISO-induced MI by suppressing oxidative stress and inflammation. PP extracts may play an important cardioprotective role in the treatment of MI with their antioxidant and anti-inflammatory effects.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140490170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.55262/fabadeczacilik.1346469
Kisan Jadhav, Shivani Gharat, Shradha B Adhalrao
Electrospinning process was used for constructing the bilayer nanofibers scaffold using drug and oil containing polymeric emulsion for synergistic activity against bacterial wound infections. The characterization was done for morphology, chemical composition, thermal behaviour, porosity, swelling ratio, drug release, in vitro permeation, ex vivo studies, and antimicrobial activity. In vitro drug permeation study, exhibited initial rapid release of MUP upto 53% in the first 6 hrs and following a slower sustained release achieving 92.17% drug release in 72 hours whereas TEO showed release in 10 hrs with initial burst release followed by sustained release achieving 85.45% release. The ex vivo skin permeation data revealed greater permeability compared to conventional films. Antibacterial activity of the bilayer nanofiber scaffold was found to be greater than that of single layer MUP and TEO scaffold as zone of inhibition of MUP-PCL-TEO-PVA bilayer scaffold was greater which indicates the synergistic activity of MUP and TEO.
{"title":"MUP-PCL-TEO-PVA Bilayer Nanofiber Scaffold Incorporated with Mupirocin and Thyme Essential Oil for Synergistic Activity Against Bacterial Wound Infections.","authors":"Kisan Jadhav, Shivani Gharat, Shradha B Adhalrao","doi":"10.55262/fabadeczacilik.1346469","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1346469","url":null,"abstract":"Electrospinning process was used for constructing the bilayer nanofibers scaffold using drug and oil \u0000containing polymeric emulsion for synergistic activity against bacterial wound infections. The \u0000characterization was done for morphology, chemical composition, thermal behaviour, porosity, \u0000swelling ratio, drug release, in vitro permeation, ex vivo studies, and antimicrobial activity. In vitro \u0000drug permeation study, exhibited initial rapid release of MUP upto 53% in the first 6 hrs and \u0000following a slower sustained release achieving 92.17% drug release in 72 hours whereas TEO showed \u0000release in 10 hrs with initial burst release followed by sustained release achieving 85.45% release. The \u0000ex vivo skin permeation data revealed greater permeability compared to conventional films. \u0000Antibacterial activity of the bilayer nanofiber scaffold was found to be greater than that of single layer \u0000MUP and TEO scaffold as zone of inhibition of MUP-PCL-TEO-PVA bilayer scaffold was greater \u0000which indicates the synergistic activity of MUP and TEO.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-04DOI: 10.55262/fabadeczacilik.1356769
Basri SATILMIŞ
Cancer stem cells play an important role in resistance to therapy, invasion, metastasis, and recurrence. CD44 is one of the well-known surface markers for hepatocellular carcinoma and its expression level is related to poor survival and a high recurrence rate. The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. The goal of the present study was to investigate whether PEITC regulates the cancer stem cell phenotype of SNU449 cells. Here, cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. Additionally, Caspase 3, CD44, Akt/mTOR, and p38/STAT3 protein expression levels were measured by western blotting. We found that compared to control confluence, gap fill, and migration rate were increased while half gap time was decreased in PEITC-treated cells. Compared to control-treated cells CD44 (3.2 fold) and p-STAT3 (2.44 fold) protein expressions were upregulated in PEITC-treated cells. Results of this study suggest that STAT3-mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells.
{"title":"Phenethyl isothiocyanate Regulates the Cancer Stem Cell Phenotype of SNU449 Hepatocellular Carcinoma Cells via STAT3-CD44 Axis","authors":"Basri SATILMIŞ","doi":"10.55262/fabadeczacilik.1356769","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1356769","url":null,"abstract":"Cancer stem cells play an important role in resistance to therapy, invasion, metastasis, and recurrence. CD44 is one of the well-known surface markers for hepatocellular carcinoma and its expression level is related to poor survival and a high recurrence rate. The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. The goal of the present study was to investigate whether PEITC regulates the cancer stem cell phenotype of SNU449 cells. Here, cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. Additionally, Caspase 3, CD44, Akt/mTOR, and p38/STAT3 protein expression levels were measured by western blotting. We found that compared to control confluence, gap fill, and migration rate were increased while half gap time was decreased in PEITC-treated cells. Compared to control-treated cells CD44 (3.2 fold) and p-STAT3 (2.44 fold) protein expressions were upregulated in PEITC-treated cells. Results of this study suggest that STAT3-mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135647380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.55262/fabadeczacilik.1354775
Merve DENİZALTI
Abstract
Chronic wounds are one of the common and serious diabetic complications that also impose a significant financial burden on society. A comprehensive treatment for chronic wounds has not yet been found and new treatment recommendations are needed. The beneficial effects of hydrogen sulfide (H2S) on wound healing have previously been demonstrated in healthy or diabetic animal models. H2S has also been found to accelerate wound closure in cells and animal models. H2S has been shown to be beneficial in diabetic wound healing, but their effect on wound healing under diabetic conditions has not yet been elucidated. In this study; we investigated the effects of H2S and reactive oxygen species (ROS) on wound healing in fibroblast under high glucose conditions. We used 2,3-bis-(2-methoxy- -nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and scratch migration assay to investigate fibroblast cell viability and wound healing migration. We showed that H2S enhanced wound healing in fibroblasts incubated with high glucose by increasing cell viability, proliferation, migration and attenuating ROS. According to our results, exogenous H2S reduced oxidative stress during wound repair. In conclusion, H2S accelerated wound healing, which may be related to the inhibition of oxidative stress.
{"title":"Hidrojen Sülfür hiperglisemik koşullar altında fibroblastlarda reaktif oksijen türevlerini azaltarak yara iyileşmesini destekler","authors":"Merve DENİZALTI","doi":"10.55262/fabadeczacilik.1354775","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1354775","url":null,"abstract":"Abstract
 
 Chronic wounds are one of the common and serious diabetic complications that also impose a significant financial burden on society. A comprehensive treatment for chronic wounds has not yet been found and new treatment recommendations are needed. The beneficial effects of hydrogen sulfide (H2S) on wound healing have previously been demonstrated in healthy or diabetic animal models. H2S has also been found to accelerate wound closure in cells and animal models. H2S has been shown to be beneficial in diabetic wound healing, but their effect on wound healing under diabetic conditions has not yet been elucidated. In this study; we investigated the effects of H2S and reactive oxygen species (ROS) on wound healing in fibroblast under high glucose conditions. We used 2,3-bis-(2-methoxy- -nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and scratch migration assay to investigate fibroblast cell viability and wound healing migration. We showed that H2S enhanced wound healing in fibroblasts incubated with high glucose by increasing cell viability, proliferation, migration and attenuating ROS. According to our results, exogenous H2S reduced oxidative stress during wound repair. In conclusion, H2S accelerated wound healing, which may be related to the inhibition of oxidative stress.","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135902421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Puberte prekoks (PP), kızlarda sekonder cinsel özelliklerin sekiz yaşından önce başlamasıdır. Hipotalamus hipofiz-gonad ekseninin erken aktivasyonu nedeniyle "merkezi puberte prekoks (CPP)" meydana gelir. "Periferik puberte prekoks (PPP)" hipotalamus-hipofiz-gonad (HPG) aksına bağlı olmayan daha nadir görülen farklı bir durumdur ve periferik nedenlere bağlıdır. Metabolomiks, küçük molekül metabolitlerinin (
{"title":"Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty","authors":"Aylin BALCI ÖZYURT, Tuba REÇBER, Emirhan NEMUTLU, Derya BULUŞ, Sedef KIR, Belma KOÇER GÜMÜŞEL, Pınar ERKEKOĞLU","doi":"10.55262/fabadeczacilik.1344851","DOIUrl":"https://doi.org/10.55262/fabadeczacilik.1344851","url":null,"abstract":"Puberte prekoks (PP), kızlarda sekonder cinsel özelliklerin sekiz yaşından önce başlamasıdır. Hipotalamus hipofiz-gonad ekseninin erken aktivasyonu nedeniyle \"merkezi puberte prekoks (CPP)\" meydana gelir. \"Periferik puberte prekoks (PPP)\" hipotalamus-hipofiz-gonad (HPG) aksına bağlı olmayan daha nadir görülen farklı bir durumdur ve periferik nedenlere bağlıdır. Metabolomiks, küçük molekül metabolitlerinin (","PeriodicalId":36004,"journal":{"name":"Fabad Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135296134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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