Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.544
Leili Osanloo, H. Zeighami, F. Haghi, R. Shapouri, Rasoul Shokri
{"title":"Multidrug-Resistant Strains of Acinetobacter baumannii from Intensive Care Unit Patients Show Genetic Diversity and Distribution of Genes Associated with Aminoglycoside Resistance","authors":"Leili Osanloo, H. Zeighami, F. Haghi, R. Shapouri, Rasoul Shokri","doi":"10.30699/jambs.30.143.544","DOIUrl":"https://doi.org/10.30699/jambs.30.143.544","url":null,"abstract":"","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41467150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.570
Z. Khajali, Nasibeh Mohammadi, Raheleh Kaviani, H. Pouraliakbar, N. Parsa
10.30699/jambs.30.143.570 We herein describe a 34-year-old woman with a persistent left superior vena cava (PLSVC) impinging on the posterior wall of the left atrium, subdivided by a membrane at the distal part of the impingement. In this patient, a much dilated coronary sinus due to the PLSVC resulted in a membrane-like structure in the posterior wall of the left atrium with the appearance of the cor triatriatum in transthoracic echocardiography (ie, a pseudo cor triatriatum ). Although the PLSVC is a common malformation of the superior systemic vein, its aforementioned presentation is rare.
{"title":"Pseudo Cor Triatriatum Sinistrum due to Dilated Coronary Sinus in Association with Supramitral Ring","authors":"Z. Khajali, Nasibeh Mohammadi, Raheleh Kaviani, H. Pouraliakbar, N. Parsa","doi":"10.30699/jambs.30.143.570","DOIUrl":"https://doi.org/10.30699/jambs.30.143.570","url":null,"abstract":"10.30699/jambs.30.143.570 We herein describe a 34-year-old woman with a persistent left superior vena cava (PLSVC) impinging on the posterior wall of the left atrium, subdivided by a membrane at the distal part of the impingement. In this patient, a much dilated coronary sinus due to the PLSVC resulted in a membrane-like structure in the posterior wall of the left atrium with the appearance of the cor triatriatum in transthoracic echocardiography (ie, a pseudo cor triatriatum ). Although the PLSVC is a common malformation of the superior systemic vein, its aforementioned presentation is rare.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49439791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.477
Sepideh Mirzaei, Kiavash Hushmandi, M. Entezari, A. Bahonar, M. Raei, M. Akbari
10.30699/jambs.30.143.477 Background & Objective: Mesenchymal stem cells (MSCs) promote metastasis in colorectal cancer; however, the mechanism underlying this process is not fully understood. Epithelial to mesenchymal transition (EMT) is a key step in tumor acquisition of metastatic phenotype. We aimed to investigate the effect of MSCs on the expression of EMT markers, as well as cancer stem cell markers in HT-29 colorectal cancer cells. Materials & Methods: MSCs were isolated from bone marrow tissue, and their multi potency was confirmed. The HT-29 cell line was prepared and co-cultured with MSCs for 3 days using 6-well transwell co-culture plates (membrane pore size: 0.4 µm). Cell morphology was observed by inverted microscopy. The expression levels of EMT-related genes, namely E- cadherin, Vimentin, and β -catenin, were investigated by the RT-qPCR method. Also, the surface expression levels of CD44 and CD133 cancer stem cell markers were analyzed by flow cytometry. Results: The co-culture of HT-29 cells with bone marrow-derived MSCs resulted in changes in cell morphology from epithelial to mesenchymal forms. The expression of mesenchymal stem cell markers, namely Vimentin and β -catenin, were significantly increased (2.25 and 1.83 folds, respectively), while the expression of the epithelial marker, E-cadherin, was reduced (0.3 folds). The expression of CD133 was also increased (51.5%). Conclusion: Tumor-resident mesenchymal stem cells can promote colorectal cancer metastasis inducing EMT as well as increasing cancer stem cell frequency in the tumor microenvironment. It seems that direct contact between MSCs and colorectal cancer cells is not required for the interaction. Our findings may help scientists to find effective strategies against cancer metastasis by targeting tumor-resident MSCs.
{"title":"Mesenchymal Stem Cells Trigger Epithelial to Mesenchymal Transition in the HT-29 Colorectal Cancer Cell Line","authors":"Sepideh Mirzaei, Kiavash Hushmandi, M. Entezari, A. Bahonar, M. Raei, M. Akbari","doi":"10.30699/jambs.30.143.477","DOIUrl":"https://doi.org/10.30699/jambs.30.143.477","url":null,"abstract":"10.30699/jambs.30.143.477 Background & Objective: Mesenchymal stem cells (MSCs) promote metastasis in colorectal cancer; however, the mechanism underlying this process is not fully understood. Epithelial to mesenchymal transition (EMT) is a key step in tumor acquisition of metastatic phenotype. We aimed to investigate the effect of MSCs on the expression of EMT markers, as well as cancer stem cell markers in HT-29 colorectal cancer cells. Materials & Methods: MSCs were isolated from bone marrow tissue, and their multi potency was confirmed. The HT-29 cell line was prepared and co-cultured with MSCs for 3 days using 6-well transwell co-culture plates (membrane pore size: 0.4 µm). Cell morphology was observed by inverted microscopy. The expression levels of EMT-related genes, namely E- cadherin, Vimentin, and β -catenin, were investigated by the RT-qPCR method. Also, the surface expression levels of CD44 and CD133 cancer stem cell markers were analyzed by flow cytometry. Results: The co-culture of HT-29 cells with bone marrow-derived MSCs resulted in changes in cell morphology from epithelial to mesenchymal forms. The expression of mesenchymal stem cell markers, namely Vimentin and β -catenin, were significantly increased (2.25 and 1.83 folds, respectively), while the expression of the epithelial marker, E-cadherin, was reduced (0.3 folds). The expression of CD133 was also increased (51.5%). Conclusion: Tumor-resident mesenchymal stem cells can promote colorectal cancer metastasis inducing EMT as well as increasing cancer stem cell frequency in the tumor microenvironment. It seems that direct contact between MSCs and colorectal cancer cells is not required for the interaction. Our findings may help scientists to find effective strategies against cancer metastasis by targeting tumor-resident MSCs.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46489747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.501
Sajjad Biglari, A. Biglari, S. Mazloomzadeh
10.30699/jambs.30.143.501 Background & Objective: Consanguinity increases the incidence of genetic disorders. The frequency of consanguinity varies in different societies. There was no data regarding the frequency of consanguinity in Zanjan province. This study aimed to describe the prevalence of consanguineous unions in the parents of children with genetic disorders and its related factors in Zanjan, Iran. Materials & Methods: This cross-sectional study included children with genetic diseases referring to the medical genetics clinic in Zanjan's Musavi Hospital during 2014-2018. Data including consanguineous unions in families (up to three previous generations), types of genetic illnesses, child and parents' age, parental educational level, and occupation were collected and analyzed using descriptive statistics, independent t-test, and chi-square test. Results: Of the 87 children, 41 (47.7%) were male, and 50 (59.5%) resided in urban areas. The mean age of the children was 6.5 years. The educational level of 56.6% of fathers and 50.6% of mothers were highschool diploma or higher. The parents of 44 children (51.8%) had consanguineous unions. The most common type of consanguineous union was between first cousins. The nature of the genetic disorders in 63 (72.4%) of children was molecular. The proportion of consanguinity was significantly higher in parents of children with molecular than chromosomal disorder (P<0.0001). Consanguinity had also a significant relationship with the children's age (P=0.04). Conclusion: This study's outcomes illustrate that parents of more than half of children with genetic disorders had consanguinity and the frequency of consanguinity was more common in parents of children with molecular disease than chromosomal disorder. Given that, it is necessary to avoid consanguineous marriage as much as possible, and families with a history of molecular genetic disease, should be informed of the possible consequences.
{"title":"The Frequency of Consanguinity and Its Related Factors in Parents of Children with Genetic Disorders","authors":"Sajjad Biglari, A. Biglari, S. Mazloomzadeh","doi":"10.30699/jambs.30.143.501","DOIUrl":"https://doi.org/10.30699/jambs.30.143.501","url":null,"abstract":"10.30699/jambs.30.143.501 Background & Objective: Consanguinity increases the incidence of genetic disorders. The frequency of consanguinity varies in different societies. There was no data regarding the frequency of consanguinity in Zanjan province. This study aimed to describe the prevalence of consanguineous unions in the parents of children with genetic disorders and its related factors in Zanjan, Iran. Materials & Methods: This cross-sectional study included children with genetic diseases referring to the medical genetics clinic in Zanjan's Musavi Hospital during 2014-2018. Data including consanguineous unions in families (up to three previous generations), types of genetic illnesses, child and parents' age, parental educational level, and occupation were collected and analyzed using descriptive statistics, independent t-test, and chi-square test. Results: Of the 87 children, 41 (47.7%) were male, and 50 (59.5%) resided in urban areas. The mean age of the children was 6.5 years. The educational level of 56.6% of fathers and 50.6% of mothers were highschool diploma or higher. The parents of 44 children (51.8%) had consanguineous unions. The most common type of consanguineous union was between first cousins. The nature of the genetic disorders in 63 (72.4%) of children was molecular. The proportion of consanguinity was significantly higher in parents of children with molecular than chromosomal disorder (P<0.0001). Consanguinity had also a significant relationship with the children's age (P=0.04). Conclusion: This study's outcomes illustrate that parents of more than half of children with genetic disorders had consanguinity and the frequency of consanguinity was more common in parents of children with molecular disease than chromosomal disorder. Given that, it is necessary to avoid consanguineous marriage as much as possible, and families with a history of molecular genetic disease, should be informed of the possible consequences.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46740661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.553
Roghayeh Rashidi, A. Afshari, H. Mollazadeh, A. Hosseini
10.30699/jambs.30.143.553 Background & Objective: Rheum turkestanicum ( R. turkestanicum ) has been known to reduce inflammation and has antioxidant properties such as protective effect in neurons. This study aimed to determine the effects of R. turkestanicum on neuronal toxicity induced by the pro-parkinsonian neurotoxin 6-hydroxydopamine (6-OHDA) in neuroblastoma SH-SY5Y cells. Materials & Methods: MTT and DNA fragmentation by PI staining (sub-G1 peak) assays were used to determine cell viability and induction of apoptosis, respectively. Fluorimetry methods measured lipid peroxidation malondialdehyde (MDA) and reactive oxygen species (ROS) levels. The amount of glutathione (GSH) and the activity of superoxide dismutase (SOD) were measured by DTNB (5, 5′ -Dithiobis (2-nitrobenzoic acid) and pyrogallol respectively. Results: Pretreatment with 12.5 to 100 μg/mL of R. turkestanicum extract for 24 hours attenuated 6- OHDA (final concentration 42.5 μg/mL) -induced cytotoxicity. Also, the pretreatment of SH-SY5Y cells with R. turkestanicum inhibited 6-OHDA-stimulated apoptosis in a dose-dependent manner. Additionally, R. turkestanicum extract repressed 6-OHDA-induced oxidative stress as measured by the MDA, ROS, GSH, and SOD levels. Conclusion: The findings suggest that R. turkestanicum extract has neuroprotective activity on 6-OHDA-induced neuronal toxicity of neuroblastoma cells.
{"title":"Rheum turkestanicum Janisch Root Extract Mitigates 6-OHDA-Induced Neuronal Toxicity Against Human Neuroblastoma SH-SY5Y Cells","authors":"Roghayeh Rashidi, A. Afshari, H. Mollazadeh, A. Hosseini","doi":"10.30699/jambs.30.143.553","DOIUrl":"https://doi.org/10.30699/jambs.30.143.553","url":null,"abstract":"10.30699/jambs.30.143.553 Background & Objective: Rheum turkestanicum ( R. turkestanicum ) has been known to reduce inflammation and has antioxidant properties such as protective effect in neurons. This study aimed to determine the effects of R. turkestanicum on neuronal toxicity induced by the pro-parkinsonian neurotoxin 6-hydroxydopamine (6-OHDA) in neuroblastoma SH-SY5Y cells. Materials & Methods: MTT and DNA fragmentation by PI staining (sub-G1 peak) assays were used to determine cell viability and induction of apoptosis, respectively. Fluorimetry methods measured lipid peroxidation malondialdehyde (MDA) and reactive oxygen species (ROS) levels. The amount of glutathione (GSH) and the activity of superoxide dismutase (SOD) were measured by DTNB (5, 5′ -Dithiobis (2-nitrobenzoic acid) and pyrogallol respectively. Results: Pretreatment with 12.5 to 100 μg/mL of R. turkestanicum extract for 24 hours attenuated 6- OHDA (final concentration 42.5 μg/mL) -induced cytotoxicity. Also, the pretreatment of SH-SY5Y cells with R. turkestanicum inhibited 6-OHDA-stimulated apoptosis in a dose-dependent manner. Additionally, R. turkestanicum extract repressed 6-OHDA-induced oxidative stress as measured by the MDA, ROS, GSH, and SOD levels. Conclusion: The findings suggest that R. turkestanicum extract has neuroprotective activity on 6-OHDA-induced neuronal toxicity of neuroblastoma cells.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46747541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.536
M. Soltan-dallal, Z. Rajabi, Sarah SHARİFİ YAZDİ, Parisa Torabi-Bonab, Alireza Monadi Sefidan, Sina Sharifi Yazdi
10.30699/jambs.30.143.536 Background & Objective: Breast milk contains nutrients such as carbohydrates, essential fatty acids, proteins, vitamins, minerals, and a source of communal bacteria with probiotic potential that is very effective in the prevention and treatment of neonatal infections. The aim of this study was the evaluation of probiotic properties of lactobacilli in breast milk and their inhibitory effect on pathogenic bacteria of the gastrointestinal tracts. Materials & Methods: In a cross-sectional descriptive study, during 10 months from January to October 2018, 100 breast milk samples were collected by referring to health centers after isolation. Lactobacilli strains were evaluated based on morphological characteristics, catalase, and hot staining tests, survival tests in acidic conditions, and bile salt tolerance to evaluate probiotic properties. Antibiotic resistance of probiotic strains and ability to inhibit pathogenic bacteria was evaluated by well method and growth inhibition zone. Results: 122 lactobacilli belonging to 12 species were identified from 100 samples of breast milk by phototypical methods. The predominant species belonging to casei and other lactobacilli were Fermentum, Plantarum, and Gasseri, respectively.The highest antibiotic resistance was related to vancomycin (63.15%). The 3 isolates L4, L14 and L16 were able to strongly inhibit all the studied gastrointestinal pathogens. Conclusion: Breast milk is a rich source of beneficial probiotic lactobacilli, which can be useful in breast milk for infants who are not breastfed to prevent neonatal infections.
{"title":"Evaluation of Probiotic Properties of Lactobacilli in Breast Milk and Their Inhibitory Effect on Pathogenic Bacteria of the Gastrointestinal Tracts","authors":"M. Soltan-dallal, Z. Rajabi, Sarah SHARİFİ YAZDİ, Parisa Torabi-Bonab, Alireza Monadi Sefidan, Sina Sharifi Yazdi","doi":"10.30699/jambs.30.143.536","DOIUrl":"https://doi.org/10.30699/jambs.30.143.536","url":null,"abstract":"10.30699/jambs.30.143.536 Background & Objective: Breast milk contains nutrients such as carbohydrates, essential fatty acids, proteins, vitamins, minerals, and a source of communal bacteria with probiotic potential that is very effective in the prevention and treatment of neonatal infections. The aim of this study was the evaluation of probiotic properties of lactobacilli in breast milk and their inhibitory effect on pathogenic bacteria of the gastrointestinal tracts. Materials & Methods: In a cross-sectional descriptive study, during 10 months from January to October 2018, 100 breast milk samples were collected by referring to health centers after isolation. Lactobacilli strains were evaluated based on morphological characteristics, catalase, and hot staining tests, survival tests in acidic conditions, and bile salt tolerance to evaluate probiotic properties. Antibiotic resistance of probiotic strains and ability to inhibit pathogenic bacteria was evaluated by well method and growth inhibition zone. Results: 122 lactobacilli belonging to 12 species were identified from 100 samples of breast milk by phototypical methods. The predominant species belonging to casei and other lactobacilli were Fermentum, Plantarum, and Gasseri, respectively.The highest antibiotic resistance was related to vancomycin (63.15%). The 3 isolates L4, L14 and L16 were able to strongly inhibit all the studied gastrointestinal pathogens. Conclusion: Breast milk is a rich source of beneficial probiotic lactobacilli, which can be useful in breast milk for infants who are not breastfed to prevent neonatal infections.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43039545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.30699/jambs.30.143.566
M. Ghoncheh, Forood Salehi
{"title":"Repair of a Scalp Defect with Punctate Skull Fenestration and Skin Grafting","authors":"M. Ghoncheh, Forood Salehi","doi":"10.30699/jambs.30.143.566","DOIUrl":"https://doi.org/10.30699/jambs.30.143.566","url":null,"abstract":"","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48815102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.30699/jambs.30.142.388
H. Khosravi, A. Doosti-Irani, H. Bouraghi, S. Nikzad
{"title":"Investigation of Gold Nanoparticles Effects in Radiation Therapy of Cancer: A Systematic Review","authors":"H. Khosravi, A. Doosti-Irani, H. Bouraghi, S. Nikzad","doi":"10.30699/jambs.30.142.388","DOIUrl":"https://doi.org/10.30699/jambs.30.142.388","url":null,"abstract":"","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48550511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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