Pub Date : 2021-02-16DOI: 10.4194/2459-1831-v5_2_01
S. Sarjito, A. Sabdono
Indonesian shrimp cultures are threatened by vibriosis. Some traditional brackish water ponds remained along the north coast of Central Java after the disease outbreaks destroyed the shrimp culture. This study aimed to discover the Vibrio diversity associated with shrimp vibriosis in traditional brackish water ponds. An exploratory method with purposive sampling was used in this study. Twenty-four shrimps presumably infected with vibriosis were collected from two district regions on the north coast of Central Java in July–September 2018. The bacteria associated in shrimp vibriosis were isolated from the telson and inner part of the hepatopancreas with TCBS medium. Forty-one bacteria associated with shrimp vibriosis were obtained, and then repetitive-polymerase chain reaction (rep-PCR) was performed to obtain Vibrio strains. On the basis of rep-PCR results, five respresentative strains were selected for further study. The results of 16S rDNA sequence analysis showed that the JKP03, JKP05, JKP19, JKM01, and JKM06 isolates were closely related to Vibrio rotiferianus, Vibrio diabolicus, Vibrio parahaemolyticus, Vibrio alginolyticus, and Shewanella algae, respectively. Vibrio biodiversity in shrimp vibriosis was high. These results confirmed that traditional shrimp farming was susceptible to vibriosis. Therefore, control methods such as vaccines, probiotics, and immunostimulant formulas must be developed to prevent and control the outbreak of shrimp vibriosis in traditional brackish water pond.
{"title":"Associated Vibrio Species in Shrimp Vibriosis from Traditional Brackish Water Pond in the North Coastal of Central Java, Indonesia","authors":"S. Sarjito, A. Sabdono","doi":"10.4194/2459-1831-v5_2_01","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_01","url":null,"abstract":"Indonesian shrimp cultures are threatened by vibriosis. Some traditional brackish water ponds remained along the north coast of Central Java after the disease outbreaks destroyed the shrimp culture. This study aimed to discover the Vibrio diversity associated with shrimp vibriosis in traditional brackish water ponds. An exploratory method with purposive sampling was used in this study. Twenty-four shrimps presumably infected with vibriosis were collected from two district regions on the north coast of Central Java in July–September 2018. The bacteria associated in shrimp vibriosis were isolated from the telson and inner part of the hepatopancreas with TCBS medium. Forty-one bacteria associated with shrimp vibriosis were obtained, and then repetitive-polymerase chain reaction (rep-PCR) was performed to obtain Vibrio strains. On the basis of rep-PCR results, five respresentative strains were selected for further study. The results of 16S rDNA sequence analysis showed that the JKP03, JKP05, JKP19, JKM01, and JKM06 isolates were closely related to Vibrio rotiferianus, Vibrio diabolicus, Vibrio parahaemolyticus, Vibrio alginolyticus, and Shewanella algae, respectively. Vibrio biodiversity in shrimp vibriosis was high. These results confirmed that traditional shrimp farming was susceptible to vibriosis. Therefore, control methods such as vaccines, probiotics, and immunostimulant formulas must be developed to prevent and control the outbreak of shrimp vibriosis in traditional brackish water pond.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49497858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-09DOI: 10.4194/2459-1831-V5_1_05
Muradiye Karasu Ayata, S. Karakuş, M. Gaffaroğlu
The aim of this study was to determine chromosomal characteristics of Rhodeus amarus (Block, 1782) from Turkey by conventional procedures (Giemsa, C-banding and Ag-NOR staining). Metaphase chromosomes were obtained from the head kidney cells. The diploid number was found as 48 and the fundamental number as 76. Chromosomes were morphologically characterized as metacentric (four pairs), submetacentric (10 pairs) and subtelo-acrocentric (10 pairs). C-bands were found to occur on the pericentromeric regions of most of the chromosomes and a single AgNOR was observed on Silver stained metaphases. The results may expand the knowledge on chromosomal features of bitterlings.
本研究的目的是通过常规方法(Giemsa, c -带和Ag-NOR染色)确定土耳其Rhodeus amarus (Block, 1782)的染色体特征。从头肾细胞中获得中期染色体。二倍体数为48，基数为76。染色体形态上分别为4对、10对和10对。c带出现在大多数染色体的中心周围区域，在银色染色中期观察到单个AgNOR。这一结果可能会扩大对苦鱼染色体特征的认识。
{"title":"Karyology of Rhodeus amarus (Block, 1782) (Teleostei, Acheilognathidae) from Turkey","authors":"Muradiye Karasu Ayata, S. Karakuş, M. Gaffaroğlu","doi":"10.4194/2459-1831-V5_1_05","DOIUrl":"https://doi.org/10.4194/2459-1831-V5_1_05","url":null,"abstract":"The aim of this study was to determine chromosomal characteristics of Rhodeus amarus (Block, 1782) from Turkey by conventional procedures (Giemsa, C-banding and Ag-NOR staining). Metaphase chromosomes were obtained from the head kidney cells. The diploid number was found as 48 and the fundamental number as 76. Chromosomes were morphologically characterized as metacentric (four pairs), submetacentric (10 pairs) and subtelo-acrocentric (10 pairs). C-bands were found to occur on the pericentromeric regions of most of the chromosomes and a single AgNOR was observed on Silver stained metaphases. The results may expand the knowledge on chromosomal features of bitterlings.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48575437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.4194/2459-1831-v5_2_02
B. Khamnamtong, Sirikan Prasertlux, Sirithorn Janpoom, S. Klinbunga
The basic information on genetic diversity and population structure is essential for the construction of appropriate management schemes leading to sustainable fisheries of the blue swimming crab (Portunus pelagicus). Here, genetic heterogeneity of P. pelagicus (N=174) was examined by single-strand conformational polymorphism (SSCP) analysis of mitochondrial cytochrome c oxidase subunit I (PpCOI270). Seven SSCP genotypes were found across all investigated samples. The average genetic distance between pairs of geographic samples was 0.0014-0.7247. Significant geographic heterogeneity (P<0.05) and restricted levels of female gene flow between paired samples (0.03-1.60 individuals per generation) were observed except between Chanthaburi - Prachuap Kriri Khan and Ranong - Krabi (P>0.05; 6.54 and 16.17 individuals per generation) located in the same coastal regions. Therefore, the gene pool of P. pelagicus in Thai waters was genetically differentiated to different stocks even though it is biologically regarded as a potential dispersal species. Five geographic samples of P. pelagicus in Thai waters could be differentiated to three genetic stocks; Chanthaburi and Suratthani (stock A), Prachuap Khiri Khan (stock B) and Ranong and Krabi (stock C).
{"title":"Genetic Differentiation of the Blue Swimming Crab Portunus pelagicus Along the Coastal Thai Waters Revealed by SSCP Analysis of Cytochrome c Oxidase Subunit I","authors":"B. Khamnamtong, Sirikan Prasertlux, Sirithorn Janpoom, S. Klinbunga","doi":"10.4194/2459-1831-v5_2_02","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_02","url":null,"abstract":"The basic information on genetic diversity and population structure is essential for the construction of appropriate management schemes leading to sustainable fisheries of the blue swimming crab (Portunus pelagicus). Here, genetic heterogeneity of P. pelagicus (N=174) was examined by single-strand conformational polymorphism (SSCP) analysis of mitochondrial cytochrome c oxidase subunit I (PpCOI270). Seven SSCP genotypes were found across all investigated samples. The average genetic distance between pairs of geographic samples was 0.0014-0.7247. Significant geographic heterogeneity (P<0.05) and restricted levels of female gene flow between paired samples (0.03-1.60 individuals per generation) were observed except between Chanthaburi - Prachuap Kriri Khan and Ranong - Krabi (P>0.05; 6.54 and 16.17 individuals per generation) located in the same coastal regions. Therefore, the gene pool of P. pelagicus in Thai waters was genetically differentiated to different stocks even though it is biologically regarded as a potential dispersal species. Five geographic samples of P. pelagicus in Thai waters could be differentiated to three genetic stocks; Chanthaburi and Suratthani (stock A), Prachuap Khiri Khan (stock B) and Ranong and Krabi (stock C).","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70421919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.4194/2459-1831-v5_1_04
О. Bielikova, S. Тarasjuk, A. Mruk, O. Zaloilo, A. Didenko
The genetic structure of rainbow trout farmed in Ukraine were characterized based on microsatellite loci. The selected set of SSR-markers (Simple-sequence repeatsmarkers) had a high degree of polymorphism that allowed determining the specificity of each local stock (average PIC value = 0.785 ± 0.034). The microsatellite analysis of rainbow trout from the studied stocks showed a high level of genetic diversity (uHe = 0.825 ± 0.030, PIC = 0.785 ± 0.034, I = 1.836 ± 0.127). The level of allelic diversity of the selected loci was high, where the average number of alleles per locus was 7.833, the effective number of alleles per locus was 5.687. The range of amplicon sizes of the studied loci and private alleles for each local group was determined. The cluster analysis showed the presence of three clusters. The range of genetic variability was presented in the graphical interpretation of the principal coordinates analysis (PCoA). Based on unbiased genetic distances, the Kharkiv and Transcarpathian local stocks were found to be the closest, while the Chernivtsi stock was the most distant from them.
{"title":"Microsatellite-Based Analysis of Genetic Diversity and Population Structure of Rainbow Trout (Oncorhynchus mykiss) Cultured in Ukraine","authors":"О. Bielikova, S. Тarasjuk, A. Mruk, O. Zaloilo, A. Didenko","doi":"10.4194/2459-1831-v5_1_04","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_1_04","url":null,"abstract":"The genetic structure of rainbow trout farmed in Ukraine were characterized based on microsatellite loci. The selected set of SSR-markers (Simple-sequence repeatsmarkers) had a high degree of polymorphism that allowed determining the specificity of each local stock (average PIC value = 0.785 ± 0.034). The microsatellite analysis of rainbow trout from the studied stocks showed a high level of genetic diversity (uHe = 0.825 ± 0.030, PIC = 0.785 ± 0.034, I = 1.836 ± 0.127). The level of allelic diversity of the selected loci was high, where the average number of alleles per locus was 7.833, the effective number of alleles per locus was 5.687. The range of amplicon sizes of the studied loci and private alleles for each local group was determined. The cluster analysis showed the presence of three clusters. The range of genetic variability was presented in the graphical interpretation of the principal coordinates analysis (PCoA). Based on unbiased genetic distances, the Kharkiv and Transcarpathian local stocks were found to be the closest, while the Chernivtsi stock was the most distant from them.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48891203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-25DOI: 10.4194/2459-1831-V4_2_04
S. Klinbunga, Sirikan Prasertlux, Sirithorn Janpoom, Puttawan Romgmung, B. Khamnamtong
Genes involved in ovarian development of the giant tiger shrimp (Penaeus monodon) were isolated by cDNA-AFLP analysis. In total, 75 primer combinations were screened against cDNA of different stages of ovaries (premature ovaries of juveniles and previtellogenic, vitellogenic and mature ovaries of wild intact and eyestalk-ablated broodstock; N = 6 for each group). Eighteen cDNA-AFLP fragments were cloned and sequenced. Nucleotide sequences of ten fragments significantly matched EF-1α (7e63), DEAD box ATP-dependent RNA helicase 48 (PmDdx48; 8e-61), tyrosine phosphatase n9 (1e-42), autophagy related protein Atg4-like protein (3e-36), nuclear pore complex protein nup154 (6e-20), GK14382 gene product (1e-20), L-3-hydroxyacryl coenzyme A dehydrogenase short chain (8e-13) and three different hypothetical proteins. The remaining fragments did not match any previously deposited sequence (E-value > 10-4). The full-length cDNA of PmDdx48 was successfully characterized. It was 1646 bp containing an open reading frame (ORF) of 1209 bp corresponding to 402 amino acids. PmDdx48 was up-regulated in late vitellogenic and mature ovaries of wild intact broodstock and in mature ovaries of wild eyestalk-ablated broodstock (P<0.05). Unilateral eyestalk ablation resulted in down-regulation of PmDdx48 in late vitellogenic and mature ovaries compared to that of wild intact broodstock (P<0.05).
{"title":"Isolation of Differentially Expressed Transcripts by cDNA-AFLPaba and Expression Analysis of DEAD Box ATP-Dependent RNA Helicase 48 in Ovaries of the Giant Tiger Shrimp Penaeus monodon","authors":"S. Klinbunga, Sirikan Prasertlux, Sirithorn Janpoom, Puttawan Romgmung, B. Khamnamtong","doi":"10.4194/2459-1831-V4_2_04","DOIUrl":"https://doi.org/10.4194/2459-1831-V4_2_04","url":null,"abstract":"Genes involved in ovarian development of the giant tiger shrimp (Penaeus monodon) were isolated by cDNA-AFLP analysis. In total, 75 primer combinations were screened against cDNA of different stages of ovaries (premature ovaries of juveniles and previtellogenic, vitellogenic and mature ovaries of wild intact and eyestalk-ablated broodstock; N = 6 for each group). Eighteen cDNA-AFLP fragments were cloned and sequenced. Nucleotide sequences of ten fragments significantly matched EF-1α (7e63), DEAD box ATP-dependent RNA helicase 48 (PmDdx48; 8e-61), tyrosine phosphatase n9 (1e-42), autophagy related protein Atg4-like protein (3e-36), nuclear pore complex protein nup154 (6e-20), GK14382 gene product (1e-20), L-3-hydroxyacryl coenzyme A dehydrogenase short chain (8e-13) and three different hypothetical proteins. The remaining fragments did not match any previously deposited sequence (E-value > 10-4). The full-length cDNA of PmDdx48 was successfully characterized. It was 1646 bp containing an open reading frame (ORF) of 1209 bp corresponding to 402 amino acids. PmDdx48 was up-regulated in late vitellogenic and mature ovaries of wild intact broodstock and in mature ovaries of wild eyestalk-ablated broodstock (P<0.05). Unilateral eyestalk ablation resulted in down-regulation of PmDdx48 in late vitellogenic and mature ovaries compared to that of wild intact broodstock (P<0.05).","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46231710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-27DOI: 10.4194/2459-1831-v4_2_02
S. Klinbunga, Sasithorn Petkorn, Narumon Phaonakrop, Sirithorn Janpoom, Sirikan Prasertlux, Puttawan Rongmung, S. Roytrakul, P. Menasveta, B. Khamnamtong
Testicular proteome of wild and domesticated 14- and 18-month-old broodstock of the giant tiger shrimp Penaeus monodon was examined. Among 344 differentially expressed proteins identified, 11 proteins (e.g. p97/VCP-binding protein p135, lipoxygenase homology domains 1 and dipeptidyl-peptidase, accounting for 4.95% of proteins with known functions) were found in wild broodstock but not in domesticated broodstock while 152 (68.47%) proteins were commonly found in all groups of samples. Reproduction-related proteins such as vasa-like protein, Wee1-like protein kinase, serine/threonine protein kinase, mitogen activated protein kinase kinase 2, GTP-binding protein alpha subunit, seven membrane helix receptor, nuclear receptor subfamily 3, were identified. To examine a possible role of the signal transduction system in development of testes of P. monodon, the expression level of testicular serine/arginine rich-protein kinase 3 (PmSrpk3) mRNA of domesticated juveniles and broodstock was examined and it was significantly lower than that of wild P. monodon broodstock (P<0.05). PmSrpk3 expression was significantly induced by serotonin (P<0.05) but not progesterone (P>0.05) injection. The expression profiles of PmSrpk3 indicated reduced reproductive maturation of domesticated male P. monodon and exogenous administration of serotonin may be applied for promoting the testicular development of captive P. monodon
{"title":"Comparative Proteomics for Identification of ReproductionRelated Proteins in Testes of the Giant Tiger Shrimp Penaeus monodon","authors":"S. Klinbunga, Sasithorn Petkorn, Narumon Phaonakrop, Sirithorn Janpoom, Sirikan Prasertlux, Puttawan Rongmung, S. Roytrakul, P. Menasveta, B. Khamnamtong","doi":"10.4194/2459-1831-v4_2_02","DOIUrl":"https://doi.org/10.4194/2459-1831-v4_2_02","url":null,"abstract":"Testicular proteome of wild and domesticated 14- and 18-month-old broodstock of\u0000the giant tiger shrimp Penaeus monodon was examined. Among 344 differentially\u0000expressed proteins identified, 11 proteins (e.g. p97/VCP-binding protein p135,\u0000lipoxygenase homology domains 1 and dipeptidyl-peptidase, accounting for 4.95% of\u0000proteins with known functions) were found in wild broodstock but not in domesticated\u0000broodstock while 152 (68.47%) proteins were commonly found in all groups of\u0000samples. Reproduction-related proteins such as vasa-like protein, Wee1-like protein\u0000kinase, serine/threonine protein kinase, mitogen activated protein kinase kinase 2,\u0000GTP-binding protein alpha subunit, seven membrane helix receptor, nuclear receptor\u0000subfamily 3, were identified. To examine a possible role of the signal transduction\u0000system in development of testes of P. monodon, the expression level of testicular\u0000serine/arginine rich-protein kinase 3 (PmSrpk3) mRNA of domesticated juveniles and\u0000broodstock was examined and it was significantly lower than that of wild P. monodon\u0000broodstock (P<0.05). PmSrpk3 expression was significantly induced by serotonin\u0000(P<0.05) but not progesterone (P>0.05) injection. The expression profiles of PmSrpk3\u0000indicated reduced reproductive maturation of domesticated male P. monodon and\u0000exogenous administration of serotonin may be applied for promoting the testicular\u0000development of captive P. monodon","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42188425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-24DOI: 10.4194/2459-1831-v4_2_01
Tuğba Teker, G. Albayrak, T. Akaylı, Cigdem Urku
Nine lactococcal adhesin and two pneumococcal virulence genes were characterized in twenty Lactococcus garvieae strains obtained from rainbow trout, and also one human isolate by PCR. Findings showed that all strains carried adhPsaA (522 bp), LPxTG-1 (947 bp), adhCI (490 bp), and purB (864 bp) while some of the strains had adhPav (1048 bp), LPxTG-2 (767 bp), LPxTG-3 (231 bp), adhCII (732 bp), adh (398 bp) and SP_0121 (966 bp). High nucleotide homologies (85-99%) of adhCI, adhCII, adh, adhPav, adhPsaA, LPxTG-1, LPxTG-3 involved in bacterial adhesion were determined between L. garvieae strains and reference Lg2. Genes containing polymorphisms among strains were not considered to be directly involved in bacterial pathogenesis. The amplification of LPxTG-3 only in fish isolates showed that it might be responsible for coding the host-specific virulence factor. However, undetermined amplicons demonstrated that LPxTG-4 could not be used as a host-specific gene marker. Pneumococcal purB and SP_0121 have been experimentally detected in L. garvieae genome for the first time. Consequently, both purB and SP_0121 can be used as a virulence marker for L. garvieae. Findings provided valuable knowledge about L. garvieae pathogenesis and, will contribute to identify the appropriate genomic targets to develop new therapeutics against the lactococcosis.
{"title":"Screening of Lactococcal Adhesion Genes and Two Pneumococcal Genes as Genetic Determinants of Virulence in Lactococcus garvieae Strains","authors":"Tuğba Teker, G. Albayrak, T. Akaylı, Cigdem Urku","doi":"10.4194/2459-1831-v4_2_01","DOIUrl":"https://doi.org/10.4194/2459-1831-v4_2_01","url":null,"abstract":"Nine lactococcal adhesin and two pneumococcal virulence genes were characterized\u0000in twenty Lactococcus garvieae strains obtained from rainbow trout, and also one\u0000human isolate by PCR. Findings showed that all strains carried adhPsaA (522 bp),\u0000LPxTG-1 (947 bp), adhCI (490 bp), and purB (864 bp) while some of the strains had\u0000adhPav (1048 bp), LPxTG-2 (767 bp), LPxTG-3 (231 bp), adhCII (732 bp), adh (398 bp)\u0000and SP_0121 (966 bp). High nucleotide homologies (85-99%) of adhCI, adhCII, adh,\u0000adhPav, adhPsaA, LPxTG-1, LPxTG-3 involved in bacterial adhesion were determined\u0000between L. garvieae strains and reference Lg2. Genes containing polymorphisms\u0000among strains were not considered to be directly involved in bacterial pathogenesis.\u0000The amplification of LPxTG-3 only in fish isolates showed that it might be responsible\u0000for coding the host-specific virulence factor. However, undetermined amplicons\u0000demonstrated that LPxTG-4 could not be used as a host-specific gene marker.\u0000Pneumococcal purB and SP_0121 have been experimentally detected in L. garvieae\u0000genome for the first time. Consequently, both purB and SP_0121 can be used as a\u0000virulence marker for L. garvieae. Findings provided valuable knowledge about L.\u0000garvieae pathogenesis and, will contribute to identify the appropriate genomic targets\u0000to develop new therapeutics against the lactococcosis.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48378506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-24DOI: 10.4194/2459-1831-v4_1_05
A. Vella, N. Vella
The freshwater crab, Potamon fluviatile inhabits a number of watercourses in Italy, the Balkan Peninsula and some neighbouring islands. In this study, P. fluviatile specimens (n = 176) were tissue sampled from different watercourses in Maltese archipelago and were genetically analysed to investigate the population structure of the species within this small geographical area. The DNA sequences analysed included 622 nucleotides from cytochrome oxidase c subunit 1 gene, and 3160 nucleotides from the sequence between the NADH dehydrogenase subunit 6 gene and the 16S rRNA gene. Results indicated the occurrence of two main clades, with most populations exhibiting different haplotype frequencies and were characterized by private haplotypes that radiate from the main haplotype of each clade. This led to significant pairwise differences between most of the sampled populations. On grouping populations based on their geographical region, it was found that there are significantly large genetic differences between regions, with AMOVA analyses showing that over 90% of the genetic variation is due to differences between distant water systems. The population structure and the lack of genetic connectivity found among populations inhabiting different valleys highlight the urgent need for targeted conservation management of this legally protected species that is fragmented in small isolated populations.
{"title":"First Population Genetic Structure Analysis of the Freshwater Crab Potamon fluviatile (Brachyura: Potamidae) Reveals Fragmentation at Small Geographical Scale","authors":"A. Vella, N. Vella","doi":"10.4194/2459-1831-v4_1_05","DOIUrl":"https://doi.org/10.4194/2459-1831-v4_1_05","url":null,"abstract":"The freshwater crab, Potamon fluviatile inhabits a number of watercourses in Italy, the\u0000Balkan Peninsula and some neighbouring islands. In this study, P. fluviatile specimens\u0000(n = 176) were tissue sampled from different watercourses in Maltese archipelago and\u0000were genetically analysed to investigate the population structure of the species within\u0000this small geographical area. The DNA sequences analysed included 622 nucleotides\u0000from cytochrome oxidase c subunit 1 gene, and 3160 nucleotides from the sequence\u0000between the NADH dehydrogenase subunit 6 gene and the 16S rRNA gene. Results\u0000indicated the occurrence of two main clades, with most populations exhibiting\u0000different haplotype frequencies and were characterized by private haplotypes that\u0000radiate from the main haplotype of each clade. This led to significant pairwise\u0000differences between most of the sampled populations. On grouping populations based\u0000on their geographical region, it was found that there are significantly large genetic\u0000differences between regions, with AMOVA analyses showing that over 90% of the\u0000genetic variation is due to differences between distant water systems. The population\u0000structure and the lack of genetic connectivity found among populations inhabiting\u0000different valleys highlight the urgent need for targeted conservation management of\u0000this legally protected species that is fragmented in small isolated populations.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45337270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-24DOI: 10.4194/2459-1831-v4_1_04
A. Islam, S. Basak, Z. Hossain
Haor is a wetland environment considered as a rich diversified fisheries resource with important role on ecology, economy and social structure. The present study was conducted to evaluate the diversity of fish and plankton communities in the Kishoregonj haor, Kishoreganj, Bangladesh. Fish samples were collected from the fishers and fish landing station for taxonomic study from July 2017 to December 2018. Fish were identified through direct observation and using morphometric and meristic characteristics. A total of 23 genera of phytoplankton belonging to 4 classes were identified. A total 8 genera of Bacillariaophyceae, 9 genera of Chlorophyceae, 3 genera of Cyanophyceae and 3 genera of Euglenophyceae class were listed from the study area. In total 3 groups of zooplankton were identified, i.e. PhylumRotifera, OrderCladocera and Sub-class-Copepoda in Kishoreganj haor. In the present study, a total of 79 species of fishes belonging to 27 families under 9 orders were recorded. Cypriniformes was found as the most dominant order comprising 31 species followed by Siluriformes (21) and Perciformes (15). The result of this study showed that the fish and plankton diversity indices in the Kishoreganj haor are good even some fish species are gradually decreasing.
{"title":"Fish and plankton biodiversity in the Kishoreganj haor, Kishoreganj, Bangladesh","authors":"A. Islam, S. Basak, Z. Hossain","doi":"10.4194/2459-1831-v4_1_04","DOIUrl":"https://doi.org/10.4194/2459-1831-v4_1_04","url":null,"abstract":"Haor is a wetland environment considered as a rich diversified fisheries resource with important role on ecology, economy and social structure. The present study was conducted to evaluate the diversity of fish and plankton communities in the Kishoregonj haor, Kishoreganj, Bangladesh. Fish samples were collected from the fishers and fish landing station for taxonomic study from July 2017 to December 2018. Fish were identified through direct observation and using morphometric and meristic characteristics. A total of 23 genera of phytoplankton belonging to 4 classes were identified. A total 8 genera of Bacillariaophyceae, 9 genera of Chlorophyceae, 3 genera of Cyanophyceae and 3 genera of Euglenophyceae class were listed from the study area. In total 3 groups of zooplankton were identified, i.e. PhylumRotifera, OrderCladocera and Sub-class-Copepoda in Kishoreganj haor. In the present study, a total of 79 species of fishes belonging to 27 families under 9 orders were recorded. Cypriniformes was found as the most dominant order comprising 31 species followed by Siluriformes (21) and Perciformes (15). The result of this study showed that the fish and plankton diversity indices in the Kishoreganj haor are good even some fish species are gradually decreasing.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47441067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-02DOI: 10.4194/2459-1831-v3_2_01
Uzma Hussain, K. Abbas, T. Ahmed, Iram Qadeer
{"title":"Microsatellite DNA Polymorphism of Channa marulius Inhabiting River Jhelum","authors":"Uzma Hussain, K. Abbas, T. Ahmed, Iram Qadeer","doi":"10.4194/2459-1831-v3_2_01","DOIUrl":"https://doi.org/10.4194/2459-1831-v3_2_01","url":null,"abstract":"","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42767878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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