D. N. Sari, J. Ekasari, H. Nasrullah, M. Suprayudi, A. Alimuddin
Giant gourami (Osphronemus goramy) has been known to have a high ability to utilize high dietary carbohydrates. However, its molecular information related to carbohydrate metabolism is still limited. The present study elucidated Giant gourami's liver transcriptome and molecular responses to high dietary carbohydrates. Two semi-purified diets with different levels of dietary carbohydrate, normal carbohydrate (34%, N34) and high carbohydrate (53%, H53), were offered to the fish for 60 days. A total of 87,430,553 clean reads were collected and assembled into 49,034 cDNA contigs. Annotation was performed, and a total of 33,104 unigenes were obtained. High dietary carbohydrates generally down-regulated the differentially expressed genes (DEGs). The genes expression related to glycolysis and the antioxidant system were significantly upregulated (P<0.05). Meanwhile, the genes expression related to insulin signaling and gluconeogenesis were significantly downregulated (P>0.05). The results showed that Giant gourami has several adaptabilities for high carbohydrates. The upregulation of the glycolytic, gpx, and noa1 genes, downregulation of insulin signaling, and gluconeogenic genes indicated a good glucose homeostasis maintenance in Giant gourami.
{"title":"Liver Transcriptome and Molecular Responses of Giant Gourami (Osphronemus goramy) Fed with Different Dietary Carbohydrate Levels","authors":"D. N. Sari, J. Ekasari, H. Nasrullah, M. Suprayudi, A. Alimuddin","doi":"10.4194/ga494","DOIUrl":"https://doi.org/10.4194/ga494","url":null,"abstract":"Giant gourami (Osphronemus goramy) has been known to have a high ability to utilize high dietary carbohydrates. However, its molecular information related to carbohydrate metabolism is still limited. The present study elucidated Giant gourami's liver transcriptome and molecular responses to high dietary carbohydrates. Two semi-purified diets with different levels of dietary carbohydrate, normal carbohydrate (34%, N34) and high carbohydrate (53%, H53), were offered to the fish for 60 days. A total of 87,430,553 clean reads were collected and assembled into 49,034 cDNA contigs. Annotation was performed, and a total of 33,104 unigenes were obtained. High dietary carbohydrates generally down-regulated the differentially expressed genes (DEGs). The genes expression related to glycolysis and the antioxidant system were significantly upregulated (P<0.05). Meanwhile, the genes expression related to insulin signaling and gluconeogenesis were significantly downregulated (P>0.05). The results showed that Giant gourami has several adaptabilities for high carbohydrates. The upregulation of the glycolytic, gpx, and noa1 genes, downregulation of insulin signaling, and gluconeogenic genes indicated a good glucose homeostasis maintenance in Giant gourami.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45783287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Photobacterium damselae is one of the deadliest bacterial pathogens in the world of aquaculture. P. damselae has an uncharacterized protein (UniprotKb ID: A0PB24) that can enter the host cell and induce infection in fish, according to research. Domains of unknown function are proteins that have been identified experimentally but do not have a known functional or structural domain. Targeting this uncharacterized protein, we found that this protein may have link with other virulent proteins of P. damselae. So, the main objective of the study was to reveal the functional annotation of the protein and compare the inhibitory potentiality of conventional drug (Flumequine, Oxytetracycline and Florfenicol) and four potential antibacterial compounds from Syzygium aromaticum which reported previously. The uncharacterized protein was modeled, refined, and validated in this experiment. The protein-protein interaction network suggested that this uncharacterized protein may serve as a DNA/RNA hydrase and conserved domain search predict two different domains. Furthermore, the binding affinity of conventional drugs and phytochemical compounds were calculated against the protein with the docking approaches and predict S. aromaticum inhibitory compounds bind strongly with the protein. In addition, the ADMET properties of the compounds confirmed the drug likeness properties of the four compounds.
{"title":"Functional Annotation of Uncharacterized Protein from Photobacterium damselae subsp. piscicida (Pasteurella piscicida) and Comparison of Drug Target Between Conventional Medicine and Phytochemical Compound Against Disease Treatment in Fish: An In-silico Approach","authors":"Sk Injamamul Islam, M. Jahan","doi":"10.4194/ga453","DOIUrl":"https://doi.org/10.4194/ga453","url":null,"abstract":"Photobacterium damselae is one of the deadliest bacterial pathogens in the world of aquaculture. P. damselae has an uncharacterized protein (UniprotKb ID: A0PB24) that can enter the host cell and induce infection in fish, according to research. Domains of unknown function are proteins that have been identified experimentally but do not have a known functional or structural domain. Targeting this uncharacterized protein, we found that this protein may have link with other virulent proteins of P. damselae. So, the main objective of the study was to reveal the functional annotation of the protein and compare the inhibitory potentiality of conventional drug (Flumequine, Oxytetracycline and Florfenicol) and four potential antibacterial compounds from Syzygium aromaticum which reported previously. The uncharacterized protein was modeled, refined, and validated in this experiment. The protein-protein interaction network suggested that this uncharacterized protein may serve as a DNA/RNA hydrase and conserved domain search predict two different domains. Furthermore, the binding affinity of conventional drugs and phytochemical compounds were calculated against the protein with the docking approaches and predict S. aromaticum inhibitory compounds bind strongly with the protein. In addition, the ADMET properties of the compounds confirmed the drug likeness properties of the four compounds.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46496552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper reports the mitogenome of Potamon fluviatile collected from the Maltese islands, representing the first such study on species from the subfamily Potaminae. The mitogenome was analysed through next-generation sequencing and annotated. The genome was found to contain 37 genes that include 13 protein coding genes, 22 tRNA genes, two rRNA genes and a non-coding region. The gene order was compared to that of other Potamidae species, while protein coding and rRNA genes were used to evaluate the phylogenetic position of Potamon with other species of freshwater crabs. This study contributes to the genetic resources available for the genus Potamon.
{"title":"The Mitochondrial Genome of the Freshwater Crab Potamon fluviatile, the First Sequenced Representative of the Subfamily Potaminae and Its Phylogenetic Position within Potamidae","authors":"A. Vella, N. Vella","doi":"10.4194/ga460","DOIUrl":"https://doi.org/10.4194/ga460","url":null,"abstract":"This paper reports the mitogenome of Potamon fluviatile collected from the Maltese islands, representing the first such study on species from the subfamily Potaminae. The mitogenome was analysed through next-generation sequencing and annotated. The genome was found to contain 37 genes that include 13 protein coding genes, 22 tRNA genes, two rRNA genes and a non-coding region. The gene order was compared to that of other Potamidae species, while protein coding and rRNA genes were used to evaluate the phylogenetic position of Potamon with other species of freshwater crabs. This study contributes to the genetic resources available for the genus Potamon.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48761547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, identification of the cytochrome c oxidase subunit I (COI) gene region in mitochondrial DNA of the Diplostomum specimens, which are parasitic in the eyes of Capoeta umbla fish naturally distributed in Karakaya Dam Lake, Keban Dam Lake and Lake Hazar, (Elazığ province) was performed. A total of 8 haplotypes were determined using sequence analysis of the mtCOI gene region and phylogenetic trees were constructed by determining the genetic distance based on these haplotypes by the Maximum Likelihood (ML), Neighbor Joining (NJ), Minimum Evolution (ME) and Bayes methods. As a result, the lowest genetic distance was 0,00209 and the highest genetic distance was 0,01264. Phylogenetic analysis revealed a closer relationship between haplotype 3 and haplotype 4. It has been evaluated that all the samples studied belong to Diplostomum spathaceum and that the parasite may have been spread by the main hosts between study sites.
{"title":"Identification and Molecular Investigation of Diplostomum in Capoeta umbla Caught from Freshwater Sources, Turkey","authors":"Sibel Doğan Barata, M. Dörücü, Murad Gürses","doi":"10.4194/ga454","DOIUrl":"https://doi.org/10.4194/ga454","url":null,"abstract":"In this study, identification of the cytochrome c oxidase subunit I (COI) gene region in mitochondrial DNA of the Diplostomum specimens, which are parasitic in the eyes of Capoeta umbla fish naturally distributed in Karakaya Dam Lake, Keban Dam Lake and Lake Hazar, (Elazığ province) was performed. A total of 8 haplotypes were determined using sequence analysis of the mtCOI gene region and phylogenetic trees were constructed by determining the genetic distance based on these haplotypes by the Maximum Likelihood (ML), Neighbor Joining (NJ), Minimum Evolution (ME) and Bayes methods. As a result, the lowest genetic distance was 0,00209 and the highest genetic distance was 0,01264. Phylogenetic analysis revealed a closer relationship between haplotype 3 and haplotype 4. It has been evaluated that all the samples studied belong to Diplostomum spathaceum and that the parasite may have been spread by the main hosts between study sites.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43446399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fish meal and fish oil have been the mostly used ingredients (protein and lipid respectively) in aquaculture feeds due to their excellent source of well-balanced amino acid profile and n-3 long chain polyunsaturated fatty acids (LC-PUFA) respectively. However, due to escalating prices and fluctuation in production, there are calls for alternative protein and lipid sources to be used. Plant based ingredients have been recognized as alternatives that could enhance growth performance as well as reduction in cost of production. The regulation of muscle growth by plant based lipids and protein is not fully elucidated. Growth performance and muscle growth of fish is affected by diets. Acceleration of growth through alteration of genes by diets can be useful as it can reduce cost and decrease time of production. The objective of this review is to discuss the genes that affect muscle growth of fish and how plant based ingredients affect these genes.
{"title":"Modulation of Muscle Growth Related Genes in Fish in Response to Plant Based Ingredients","authors":"C. Ayisi, Gyamfua Afriyie, Gilbert Owusu-Afriyie","doi":"10.4194/ga413","DOIUrl":"https://doi.org/10.4194/ga413","url":null,"abstract":"Fish meal and fish oil have been the mostly used ingredients (protein and lipid respectively) in aquaculture feeds due to their excellent source of well-balanced amino acid profile and n-3 long chain polyunsaturated fatty acids (LC-PUFA) respectively. However, due to escalating prices and fluctuation in production, there are calls for alternative protein and lipid sources to be used. Plant based ingredients have been recognized as alternatives that could enhance growth performance as well as reduction in cost of production. The regulation of muscle growth by plant based lipids and protein is not fully elucidated. Growth performance and muscle growth of fish is affected by diets. Acceleration of growth through alteration of genes by diets can be useful as it can reduce cost and decrease time of production. The objective of this review is to discuss the genes that affect muscle growth of fish and how plant based ingredients affect these genes.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48858739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A new record of a marble trout mtDNA haplotype known to be restricted to Adriatic basin (called marmoratus lineage within Salmo trutta complex) has been reported from Eşen Stream in the Aegean Sea basin of southeastern Turkey, based on sequence data of the mitochondrial DNA control region. The results of this study showed a single unique haplotype from this population, called MATR1. Phylogenetic analyses of this haplotype along with other haplotypes belonging to different mitochondrial DNA lineages of the S. trutta complex confirmed the existence of the marmoratus lineage in Turkey, suggesting a possible river capture between the Adriatic and Aegean Sea basins until the last (Würmian) marine regression.
{"title":"First Record of a Novel Mitochondrial DNA Haplotype of a Marble Trout Lineage of Salmo Trutta in Turkey","authors":"F. Bardakci, Nazan Acar, T. Arslan, R. Badraoui","doi":"10.4194/ga452","DOIUrl":"https://doi.org/10.4194/ga452","url":null,"abstract":"A new record of a marble trout mtDNA haplotype known to be restricted to Adriatic basin (called marmoratus lineage within Salmo trutta complex) has been reported from Eşen Stream in the Aegean Sea basin of southeastern Turkey, based on sequence data of the mitochondrial DNA control region. The results of this study showed a single unique haplotype from this population, called MATR1. Phylogenetic analyses of this haplotype along with other haplotypes belonging to different mitochondrial DNA lineages of the S. trutta complex confirmed the existence of the marmoratus lineage in Turkey, suggesting a possible river capture between the Adriatic and Aegean Sea basins until the last (Würmian) marine regression.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70422260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fakhriyeh Omidi, H. Jafaryan, R. Patimar, M. Harsij, H. Paknejad
Heavy metals are one of the most persistent pollutants in waters, and molecular biomarkers as bioindicators could uncover early warning signals of stress suffered by organisms exposed to these pollutants. This research was conducted to explore the impact of lead contaminant on expression levels of hsp70 gene as well as protein pattern and innate immune indicators of skin mucus in Common carp as a biological model. The fish were exposed to different concentrations of Pb(NO3)2 as a source of lead (0, 7.875, 15.75, and 31.5 mg/L of lead) for 14 days. After the completion of the experiment, the liver and gill tissue plus mucus samples were collected from the fish. Next, the effects of exposure to sublethal doses of lead were investigated on expression levels of hsp70 gene, protein pattern, and innate immune indicators of skin mucus. The results indicated that the expression level of hsp70 gene significantly increased (at 7.5% & 30% LC50) in the liver tissue compared to the control group (P<0.05). The protein pattern of treatment samples was different from that of the control group. Also, in the mucus innate immune parameters, the levels of alkaline phosphatase increased significantly at high concentrations of lead contamination in comparison with the control group (P<0.05). The protein levels first revealed an ascending trend (P<0.05), and then a descending trend (at 30% LC50), though it was not a significant downward trend compared with the control group (P>0.05). The results suggested that these physiological indicators in Cyprinus carpio could be used as molecular and mucosal biomarkers for ecotoxicological studies.
{"title":"Assessment of Responses of Common Carp, Cyprinus carpio, Exposed to Sub-Lethal Concentrations of Lead, by Molecular and Mucosal Biomarkers","authors":"Fakhriyeh Omidi, H. Jafaryan, R. Patimar, M. Harsij, H. Paknejad","doi":"10.4194/ga448","DOIUrl":"https://doi.org/10.4194/ga448","url":null,"abstract":"Heavy metals are one of the most persistent pollutants in waters, and molecular biomarkers as bioindicators could uncover early warning signals of stress suffered by organisms exposed to these pollutants. This research was conducted to explore the impact of lead contaminant on expression levels of hsp70 gene as well as protein pattern and innate immune indicators of skin mucus in Common carp as a biological model. The fish were exposed to different concentrations of Pb(NO3)2 as a source of lead (0, 7.875, 15.75, and 31.5 mg/L of lead) for 14 days. After the completion of the experiment, the liver and gill tissue plus mucus samples were collected from the fish. Next, the effects of exposure to sublethal doses of lead were investigated on expression levels of hsp70 gene, protein pattern, and innate immune indicators of skin mucus. The results indicated that the expression level of hsp70 gene significantly increased (at 7.5% & 30% LC50) in the liver tissue compared to the control group (P<0.05). The protein pattern of treatment samples was different from that of the control group. Also, in the mucus innate immune parameters, the levels of alkaline phosphatase increased significantly at high concentrations of lead contamination in comparison with the control group (P<0.05). The protein levels first revealed an ascending trend (P<0.05), and then a descending trend (at 30% LC50), though it was not a significant downward trend compared with the control group (P>0.05). The results suggested that these physiological indicators in Cyprinus carpio could be used as molecular and mucosal biomarkers for ecotoxicological studies.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46603519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-18DOI: 10.4194/2459-1831-v5_2_04
Shital Bhanushali, Kritika Katti, J. Ramchandani, S. Sen
We report the first barcode identification of water flea species Moina macrocopa (strain JSK1) from the Indian subcontinent. The strain isolated from a freshwater pond near Mumbai by collective efforts of CUBE (Collaborative Undergraduate Biology Education), was morphologically characterized as Moina species. Keeping up with the growing demand of affordable science in colleges and to avoid hazardous technologies, we established a home grown porcelain-abrasion DNA extraction strategy for crustaceans, and compared it against routinely used protocols employing liquid nitrogen or insect-DNA isolation kits. Our method successfully yielded high quality genomic DNA from Moina, comparable to published protocols as further analysed by restriction digestion, ligation and PCR efficiency. We also sequenced two barcoding loci namely, COI and ITS sequences, duly submitted and curated by Genbank (accession numbers MH734122.1 and MH745035.1). Molecular phylogenetic analyses identified the Indian species as Moina macrocopa and related it closely to the Russian clade. Protein prediction analyses of the variations in COI revealed that the missense mutations lie in the aliphatic membrane helices and should not affect the catalytic capacity of cytochrome oxidase. The strain JSK1 is currently disseminated and maintained collectively by CUBE students across India, towards developing it as a model system.
{"title":"A Cost-effective DNA Isolation Strategy from Crustaceans Enables the First Molecular Phylogenetic Identification of Moina macrocopa from India","authors":"Shital Bhanushali, Kritika Katti, J. Ramchandani, S. Sen","doi":"10.4194/2459-1831-v5_2_04","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_04","url":null,"abstract":"We report the first barcode identification of water flea species Moina macrocopa (strain JSK1) from the Indian subcontinent. The strain isolated from a freshwater pond near Mumbai by collective efforts of CUBE (Collaborative Undergraduate Biology Education), was morphologically characterized as Moina species. Keeping up with the growing demand of affordable science in colleges and to avoid hazardous technologies, we established a home grown porcelain-abrasion DNA extraction strategy for crustaceans, and compared it against routinely used protocols employing liquid nitrogen or insect-DNA isolation kits. Our method successfully yielded high quality genomic DNA from Moina, comparable to published protocols as further analysed by restriction digestion, ligation and PCR efficiency. We also sequenced two barcoding loci namely, COI and ITS sequences, duly submitted and curated by Genbank (accession numbers MH734122.1 and MH745035.1). Molecular phylogenetic analyses identified the Indian species as Moina macrocopa and related it closely to the Russian clade. Protein prediction analyses of the variations in COI revealed that the missense mutations lie in the aliphatic membrane helices and should not affect the catalytic capacity of cytochrome oxidase. The strain JSK1 is currently disseminated and maintained collectively by CUBE students across India, towards developing it as a model system.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47760267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-02DOI: 10.4194/2459-1831-v5_2_03
S. Marimuthu, C. S. Raju, S. Bhassu
In recent years, shrimp aquaculture production in Southeast Asia countries was highly infected by, microsporidian parasite, EHP. Recently, shrimps from farms located in Selangor were encountering growth retardation and it highly concerned us to carry out a clinical investigation in both farms (Location A and B). EHP infected P. monodon samples were collected from both locations. A total of 43 shrimp samples were collected and diagnosed via PCR using the 18S rRNA gene. Environmental parameters were found relatively higher than the advised values. It indicates that the farms are highly infected and toxic which had led to growth retardation and mortality. However, no significant differences were observed (except water temperatures) between the locations. The phylogenetic analysis expressed EHP detected from Malaysia formed in one clade and all the reference sequences clustered based on geographical continents (Asian-Latin America). Minimum-spanning network analysis shows that Malaysia samples Location A are genetically related with other Asian EHP samples even though the total positive rate of EHP infection was lower. However, samples from Location B were not clustered within the EHP clade and shows the highest genetic variance within Malaysia and among Asian samples. This preliminary investigation results confirmed the incidence of EHP outbreak in Malaysia.
{"title":"Clinical Investigation and Molecular Diagnosis of Microsporidian Enterocytozoon hepatopenaei (EHP) in Shrimps from Selangor, Malaysia","authors":"S. Marimuthu, C. S. Raju, S. Bhassu","doi":"10.4194/2459-1831-v5_2_03","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_03","url":null,"abstract":"In recent years, shrimp aquaculture production in Southeast Asia countries was highly infected by, microsporidian parasite, EHP. Recently, shrimps from farms located in Selangor were encountering growth retardation and it highly concerned us to carry out a clinical investigation in both farms (Location A and B). EHP infected P. monodon samples were collected from both locations. A total of 43 shrimp samples were collected and diagnosed via PCR using the 18S rRNA gene. Environmental parameters were found relatively higher than the advised values. It indicates that the farms are highly infected and toxic which had led to growth retardation and mortality. However, no significant differences were observed (except water temperatures) between the locations. The phylogenetic analysis expressed EHP detected from Malaysia formed in one clade and all the reference sequences clustered based on geographical continents (Asian-Latin America). Minimum-spanning network analysis shows that Malaysia samples Location A are genetically related with other Asian EHP samples even though the total positive rate of EHP infection was lower. However, samples from Location B were not clustered within the EHP clade and shows the highest genetic variance within Malaysia and among Asian samples. This preliminary investigation results confirmed the incidence of EHP outbreak in Malaysia.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42661497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-13DOI: 10.4194/2459-1831-v5_2_05
Kanika Yadav, A. K. Verma, A. Pathak, Abhishek Awasthi
White Spot Disease is one of the most devastating diseases of shrimps. Molecular interaction between shrimp receptor protein PmCBP (Chitin binding protein of Peneaus monodon) and viral envelop protein VP24 is obligatory for binding of the White Spot Syndrome Virus to the shrimp digestive tract, and failure of this anchoring leads to an ineffectual infection. This is a first study that throws light on the molecular interaction of PmCBP-VP24 complex and provides important clues for initial steps of ingression of the virus into shrimps.
{"title":"In Silico Prediction of Molecular Interaction Within PmCBP-VP24 Complex to Understand Initial Instigation of WSSV into Shrimps","authors":"Kanika Yadav, A. K. Verma, A. Pathak, Abhishek Awasthi","doi":"10.4194/2459-1831-v5_2_05","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_05","url":null,"abstract":"White Spot Disease is one of the most devastating diseases of shrimps. Molecular interaction between shrimp receptor protein PmCBP (Chitin binding protein of Peneaus monodon) and viral envelop protein VP24 is obligatory for binding of the White Spot Syndrome Virus to the shrimp digestive tract, and failure of this anchoring leads to an ineffectual infection. This is a first study that throws light on the molecular interaction of PmCBP-VP24 complex and provides important clues for initial steps of ingression of the virus into shrimps.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"5 1","pages":"87-93"},"PeriodicalIF":0.0,"publicationDate":"2021-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41735592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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