Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-2-2
S. Pechkovskaya, N. Filatova
{"title":"Phylogenetic analysis of the protein homologs of hemoxygenases HO-1 and HO-2 inferred from the transcriptomes of dinoflagellates","authors":"S. Pechkovskaya, N. Filatova","doi":"10.21685/1680-0826-2021-15-2-2","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-2-2","url":null,"abstract":"","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67888818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-1-2
I. Pozdnyakov, A. Zolotarev, S. Karpov
The aphelids, intracellular parasitoids of algae, have a life cycle similar to zoosporic fungi Chytridiomycota and Blastocladiomycota, and are positioned as a sister clade to all fungi on the recent multigene phylogenetic tree. The fungi and aphelids might possibly have a common ancestor with a complex life cycle somewhat similar to modern zoosporic fungi. To investigate this possibility we have analyzed the genes that increase expression during zoosporogenesis of the blastoclad fungus Blastocladiella emersonii based on the transcriptomic data of Vieira and Gomes (2013), and described the course of sporogenesis at the molecular level. Homologs of genes from the B. emersonii gene set were found in various lineages of the Opisthokonta group, and specifically in the transcriptome of Paraphelidium tribonematis . We calculated the percentage ratios of genes that formed common functional groups and the genes with homologs in various clades of related organisms. We found that zoospore production of a blastoclad fungus is a multi-phase process, where switching of the regulatory elements takes place. The analyzed genes are distributed as follows: 81% are common for all Opisthokonta, 16% are specific for Fungi and only 3% are common to Fungi and aphelids but none are found in the Holozoa lineage. Based on these data we propose a hypothesis on the independent origin of the life cycle in Fungi and Aphelida from a polymorphic ancestor.
{"title":"Comparative analysis of zoosporogenesis’ genes of the bastoclad Blastocladiella emersonii and the aphelid Paraphelidium tribonematis reveals the new directions of evolutionary research","authors":"I. Pozdnyakov, A. Zolotarev, S. Karpov","doi":"10.21685/1680-0826-2021-15-1-2","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-1-2","url":null,"abstract":"The aphelids, intracellular parasitoids of algae, have a life cycle similar to zoosporic fungi Chytridiomycota and Blastocladiomycota, and are positioned as a sister clade to all fungi on the recent multigene phylogenetic tree. The fungi and aphelids might possibly have a common ancestor with a complex life cycle somewhat similar to modern zoosporic fungi. To investigate this possibility we have analyzed the genes that increase expression during zoosporogenesis of the blastoclad fungus Blastocladiella emersonii based on the transcriptomic data of Vieira and Gomes (2013), and described the course of sporogenesis at the molecular level. Homologs of genes from the B. emersonii gene set were found in various lineages of the Opisthokonta group, and specifically in the transcriptome of Paraphelidium tribonematis . We calculated the percentage ratios of genes that formed common functional groups and the genes with homologs in various clades of related organisms. We found that zoospore production of a blastoclad fungus is a multi-phase process, where switching of the regulatory elements takes place. The analyzed genes are distributed as follows: 81% are common for all Opisthokonta, 16% are specific for Fungi and only 3% are common to Fungi and aphelids but none are found in the Holozoa lineage. Based on these data we propose a hypothesis on the independent origin of the life cycle in Fungi and Aphelida from a polymorphic ancestor.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67888911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-3-3
A. Potekhin, I. Nekrasova, Felicitas E. Flemming
{"title":"In shadow of Holospora – The continuous quest for new Holosporaceae members","authors":"A. Potekhin, I. Nekrasova, Felicitas E. Flemming","doi":"10.21685/1680-0826-2021-15-3-3","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-3-3","url":null,"abstract":"","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67889565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-2-3
I. Pozdnyakov, M. Berdieva, V. Kalinina, S. Pechkovskaya, O. Matantseva
Summary The marine planktonic dinoflagellate Prorocentrum cordatum (syn. Prorocentrum minimum ) is a thecate species capable of ecdysis, i.e. rearrangement of the cell covering in response to different stressors. During the process of ecdysis, P. cordatum cells shed the plasma membrane and outer amphiesmal vesicle membrane, and become immotile, covered only by thecal plates and the new plasma membrane. At this stage, the cells can be considered as thecal cysts. Then, they complete ecdysis by leaving the old thecal plates and restoring motility, i.e. excyst. To elucidate the role of vesicular transport in the cell covering rearrangement, we tested the effect of brefeldin A, the inhibitor of vesicular transport from the endoplasmic reticulum to the Golgi complex widely used in cell biology, on the cellular structure of P. cordatum and its ability to ecdyse. We found out that brefeldin A blocked vesicular trafficking from the endoplasmic reticulum to the Golgi complex, since we observed disassembly of the Golgi complex in cells treated by brefeldin A at 0.1 µg/ml for 1 h. Moreover, such a treatment did not increase mortality in P. cordatum culture at the time scale of 6 h. Nevertheless, our experiments demonstrated that brefeldin A affected neither shedding of outer membranes nor discarding of thecal plates during the stressor-induced ecdysis. Based on our findings, we conclude that vesicle trafficking from the endoplasmic reticulum to the Golgi complex and further to the plasma membrane is not essential to the process of membrane and theca shedding. However, this route still can be involved in the process of new amphiesma’ maturation, which is discussed in this work.
{"title":"Brefeldin A disturbs Golgi complex integrity but does not impair ecdysis in the dinoflagellates Prorocentrum cordatum","authors":"I. Pozdnyakov, M. Berdieva, V. Kalinina, S. Pechkovskaya, O. Matantseva","doi":"10.21685/1680-0826-2021-15-2-3","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-2-3","url":null,"abstract":"Summary The marine planktonic dinoflagellate Prorocentrum cordatum (syn. Prorocentrum minimum ) is a thecate species capable of ecdysis, i.e. rearrangement of the cell covering in response to different stressors. During the process of ecdysis, P. cordatum cells shed the plasma membrane and outer amphiesmal vesicle membrane, and become immotile, covered only by thecal plates and the new plasma membrane. At this stage, the cells can be considered as thecal cysts. Then, they complete ecdysis by leaving the old thecal plates and restoring motility, i.e. excyst. To elucidate the role of vesicular transport in the cell covering rearrangement, we tested the effect of brefeldin A, the inhibitor of vesicular transport from the endoplasmic reticulum to the Golgi complex widely used in cell biology, on the cellular structure of P. cordatum and its ability to ecdyse. We found out that brefeldin A blocked vesicular trafficking from the endoplasmic reticulum to the Golgi complex, since we observed disassembly of the Golgi complex in cells treated by brefeldin A at 0.1 µg/ml for 1 h. Moreover, such a treatment did not increase mortality in P. cordatum culture at the time scale of 6 h. Nevertheless, our experiments demonstrated that brefeldin A affected neither shedding of outer membranes nor discarding of thecal plates during the stressor-induced ecdysis. Based on our findings, we conclude that vesicle trafficking from the endoplasmic reticulum to the Golgi complex and further to the plasma membrane is not essential to the process of membrane and theca shedding. However, this route still can be involved in the process of new amphiesma’ maturation, which is discussed in this work.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67889594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-3-7
R. Puzanskiy, D. Romanyuk, A. Shavarda, V. Yemelyanov, M. Shishova
{"title":"A possible molecular mechanism of Chlamydomonas reinhardtii adaptation to different trophic conditions","authors":"R. Puzanskiy, D. Romanyuk, A. Shavarda, V. Yemelyanov, M. Shishova","doi":"10.21685/1680-0826-2021-15-3-7","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-3-7","url":null,"abstract":"","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67890777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-3-1
A. Pinevich
{"title":"Boris Vasilievich Gromov (1933–2001): a microbiologist for all seasons","authors":"A. Pinevich","doi":"10.21685/1680-0826-2021-15-3-1","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-3-1","url":null,"abstract":"","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67889925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.21685/1680-0826-2021-15-4-2
I. Telesh, E. Naumenko
{"title":"The impact of nuisance planktonic invaders on pelagic communities: a review of the Baltic Sea case studies","authors":"I. Telesh, E. Naumenko","doi":"10.21685/1680-0826-2021-15-4-2","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-4-2","url":null,"abstract":"","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67890817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.21685/1680-0826-2020-14-4-3
S. Karpov, A. Vishnyakov, P. López‐García, Natalia A. Zorina, Maria Ciobanu, Victoria S. Tcvetkova, David Moreira, Ecologie Systématique Evolution
,
,
{"title":"Morphology and molecular phylogeny of Aphelidium insulamus sp. nov. (Aphelida, Opisthosporidia)","authors":"S. Karpov, A. Vishnyakov, P. López‐García, Natalia A. Zorina, Maria Ciobanu, Victoria S. Tcvetkova, David Moreira, Ecologie Systématique Evolution","doi":"10.21685/1680-0826-2020-14-4-3","DOIUrl":"https://doi.org/10.21685/1680-0826-2020-14-4-3","url":null,"abstract":",","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46237749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21685/1680-0826-2020-14-4-8
P. Kment, J. Votýpka, A. Kostygov
1 Department of Entomology, National Museum, Prague, Czechia 2 Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice, Czechia 3 Department of Parasitology, Faculty of Science, Charles University, Prague, Czechia 4 Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia 5 Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czechia
{"title":"Pitfalls of describing new taxa in the age of on-line publications and how to get out of there","authors":"P. Kment, J. Votýpka, A. Kostygov","doi":"10.21685/1680-0826-2020-14-4-8","DOIUrl":"https://doi.org/10.21685/1680-0826-2020-14-4-8","url":null,"abstract":"1 Department of Entomology, National Museum, Prague, Czechia 2 Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice, Czechia 3 Department of Parasitology, Faculty of Science, Charles University, Prague, Czechia 4 Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia 5 Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czechia","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67888869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21685/1680-0826-2020-14-4-6
P. Safonov, Alexandr Khiutti, I. Pozdnyakov
Summary Oomycetes are a group of parasitic eukaryotic microorganisms infecting plant and animal hosts worldwide. Many of those, for example, the crop parasite Phytophthora infestans , have a considerable economic impact. Previously we revealed the unexpected diversity of ion channels that could participate in the potassium transport in oomycetes. In the present study, we investigated the effects of cesium ions, which are known to nonspecifically block the activity of membrane proteins involved in the potassium transport, on the growth of Ph. infestans mycelium and motility of its zoospores. We showed that the addition of 3–24 mM cesium chloride to the culturing medium inhibited the oomycete growth on agar plates. Moreover, the application of 6 and 12 mM CsCl to zoospores substantially reduced the velocity of their movement in a liquid medium. The results indicate an important role of potassium transport at the different stages of Ph. infestans life cycle.
卵菌是一类寄生于世界各地植物和动物宿主的真核微生物。其中许多，例如，作物寄生虫疫霉，具有相当大的经济影响。之前我们揭示了参与卵菌钾转运的离子通道的意想不到的多样性。在本研究中，我们研究了铯离子对病原菌菌丝生长及其游动孢子运动的影响，铯离子可以非特异性地阻断参与钾转运的膜蛋白的活性。结果表明，在培养基中添加3-24 mM氯化铯可抑制卵菌在琼脂板上的生长。此外，6和12 mM CsCl对游动孢子的应用大大降低了它们在液体介质中的运动速度。结果表明，钾转运在病原菌生命周期的不同阶段起着重要作用。
{"title":"Effect of cesium ions on the mycelium growth and zoospores motility in the oomycete Phytophthora infestans","authors":"P. Safonov, Alexandr Khiutti, I. Pozdnyakov","doi":"10.21685/1680-0826-2020-14-4-6","DOIUrl":"https://doi.org/10.21685/1680-0826-2020-14-4-6","url":null,"abstract":"Summary Oomycetes are a group of parasitic eukaryotic microorganisms infecting plant and animal hosts worldwide. Many of those, for example, the crop parasite Phytophthora infestans , have a considerable economic impact. Previously we revealed the unexpected diversity of ion channels that could participate in the potassium transport in oomycetes. In the present study, we investigated the effects of cesium ions, which are known to nonspecifically block the activity of membrane proteins involved in the potassium transport, on the growth of Ph. infestans mycelium and motility of its zoospores. We showed that the addition of 3–24 mM cesium chloride to the culturing medium inhibited the oomycete growth on agar plates. Moreover, the application of 6 and 12 mM CsCl to zoospores substantially reduced the velocity of their movement in a liquid medium. The results indicate an important role of potassium transport at the different stages of Ph. infestans life cycle.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67889090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：