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Effect of Eucalyptus globulus Oil on Growth of The Fungal Pathogen Fusarium oxysporum f. sp. albedinis, The Causal Agent of Date Palm Bayoud Disease in the Laboratory 桉叶油对枣椰病病原菌尖孢镰刀菌生长的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-01 DOI: 10.22268/ajpp-41.3.281284
Asmaa Noueichi,, Sleih Chibani, Ammar Othmani, Mariam Boukbach, Wadad Abdelaziz, Rima Mona
Noueichi, A., S. Chibani, A. Othmani, M. Boukbach, W. Abdelaziz and R. Mouna. 2023. Effect of Eucalyptus globulus Oil on Growth of The Fungal Pathogen Fusarium oxysporum f. sp. albedinis, The Causal Agent of Date Palm Bayoud Disease in the Laboratory. Arab Journal of Plant Protection, 41(3): 281-284. https://doi.org/10.22268/AJPP-41.3.281284 Fusarium disease of date palm caused by Fusarium oxysporum f. sp. albedinis, commonly known as Bayoud disease, is a serious threat to date palm cultivation in North African countries including Algeria. The inhibitory activity of eucalyptus oil (Eucalyptus globulus) was evaluated in the Plant Biology Laboratory at the University of Constantine, Algeria. The results obtained showed significant inhibition of fungal growth which varied between 17 and 60% based on the concentration used. The pathogen inhibitory effect was mainly due to the presence of monoterpene compounds which have a strong inhibiting property against fungi. The results obtained suggest that Eucalyptus essential oil is potentially a safe and effective alternative to chemical fungicides, but field evaluation for its ability to control date palm Bayoud disease is essential before recommending its use in farmers’ fields. Keywords: Date palm, Fusarium oxysporum f.sp. albedinis, essential oil, Eucalyptus globulus
陈建军,陈建军,陈建军,陈建军。2009。桉叶油对枣椰病病原菌尖孢镰刀菌生长的影响植物保护学报,41(3):281-284。https://doi.org/10.22268/AJPP-41.3.281284由镰孢镰刀菌(Fusarium oxysporum f. sp. albedinis)引起的枣树镰刀菌病,俗称Bayoud病,是阿尔及利亚等北非国家枣树种植的严重威胁。桉树油(eucalyptus globulus)的抑制活性在阿尔及利亚君士坦丁大学植物生物学实验室进行了评估。所获得的结果表明,真菌生长的抑制作用显著,根据所使用的浓度,其抑制作用在17%到60%之间。对病原菌的抑制作用主要是由于单萜化合物的存在,单萜化合物对真菌有很强的抑制作用。研究结果表明,桉树精油是一种安全有效的化学杀菌剂替代品,但在推荐在农民田间使用之前,必须对其防治枣椰树巴乌德病的能力进行现场评价。关键词:枣椰树;尖孢镰刀菌;白蜡树,精油,蓝桉
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引用次数: 0
Pathogenicity of the Fungus Beauveria bassiana and Cladosporium sp. on Different Life Stages of the Two Spotted Spider Mite, Tetranychus urticae Koch Under Laboratory Conditions 球孢白僵菌和枝孢菌对两种斑螨不同生命阶段的致病性研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-01 DOI: 10.22268/ajpp-41.3.314320
Alissar Shaabow, Amal Haj Hassan
Shaabow, A. and A. Haj Hassan. 2023. Pathogenicity of the Fungus Beauveria bassiana and Cladosporium sp. on Different Life Stages of the Two Spotted Spider Mite, Tetranychus urticae Koch Under Laboratory Conditions. Arab Journal of Plant Protection, 41(3): 314-320. https://doi.org/10.22268/AJPP-41.3.314320 This study was carried out in the entomology laboratory of the Agricultural Scientific Research Center in Lattakia, Syria, to evaluate and compare the effect of local isolates of the entomopathogenic fungus Beauveria bassiana and two isolates of the fungus Cladosporium spp. on different life stages of the two-spotted spider mite Tetranychus urticae during October 2021.Conidial suspension for each fungal isolate were applied at a concentration of 1×107 conidia/ml, on the different stages of mites (adults, nymphs, eggs), using the direct spraying method. The effects of each isolate were significantly different between stages. The results obtained showed that the most affected stage was the adults stage, with a death rate of 100% when treated with B. bassiana isolate, whereas it reached 72.5 and 58.5% when the two isolates of Cladosporium sp. were used. The egg stage was less sensitive to infection with fungi, where the death rate reached 59% for one of the two isolates of Cladosporium sp., 45.13% for the second isolate and 38.25% for the B. bassiana isolate. The half lethal time (LT50) was 3.82 days for the B. bassiana isolate on the adults stage, whereas isolate 2 of Cladosporium spp. was more effective on nymph and egg stages, with LT50 reached 7.77 and 8.68 days, respectively. Keywords: Tetranychus urticae, biological control, Beauveria bassiana, Cladosporium spp
A. Shaabow和A. Haj Hassan. 2023。球孢白僵菌和枝孢菌对两种斑螨不同生命阶段的致病性研究植物保护学报,41(3):314-320。https://doi.org/10.22268/AJPP-41.3.314320本研究于2021年10月在叙利亚拉塔基亚农业科学研究中心昆虫学实验室开展,评估和比较当地分离的昆虫病原真菌球孢白僵菌和两株真菌枝孢子菌对两斑叶螨不同生命阶段的影响。每个真菌分离物的分生孢子悬浮液以1×107分生孢子/ml的浓度施用于不同阶段的螨(成虫、若虫、卵),采用直接喷洒法。各菌株在不同阶段的效果有显著差异。结果表明,以球孢白僵菌为主要侵染期,用球孢白僵菌分离株处理成虫的死亡率为100%,而用枝孢菌分离株处理成虫的死亡率分别为72.5%和58.5%。卵期对真菌感染不太敏感,两株枝孢杆菌的死亡率分别为59%、45.13%和38.25%。球孢白僵菌分离物对成虫期的半致死时间(LT50)为3.82 d,而枝孢菌分离物2对若虫期和卵期的半致死时间(LT50)分别为7.77和8.68 d。关键词:荨麻疹叶螨;生物防治;球孢白僵菌
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引用次数: 0
Parasitoid Wasps of Green Lacewing, Chrysoperla carnea in Cotton Fields in Hama Governorate, Syria: New Records and Some Biological Aspects 叙利亚哈马省棉田绿草蛉寄生蜂的新记录及其生物学意义
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-01 DOI: 10.22268/ajpp-41.3.306313
Mounir Nabhan, Ziad Shiekh Khamis, Baraah Hawis
Nabhan, M., Z.Sh. Khamis and B. Hawis. 2023. Parasitoid Wasps of Green Lacewing, Chrysoperla carnea in Cotton Fields in Hama Governorate, Syria: New Records and Some Biological Aspects. Arab Journal of Plant Protection, 41(3): 306-313. https://doi.org/10.22268/AJPP-41.3.306313 This study aimed to identify parasitoid species of Chrysoperla carnea stages, and to evaluate their effect on predator’s population in the field. Three hymenopteran parasitoid species of this predator were collected and identified. Telenomus acrobates Giard (Hymenoptera: Scelionidae) was found to parasitize the eggs, and was previously recorded in northern Syria. Two other species parasitized the larva-pupa stage of the predator, and are recorded for the first time in Syria in this study. The first species is Baryscapus chrysopae (Crawford, 1915) (Hymenoptera: Eulophidae), which showed a parasitism rate of 23.08% and 28.75% of the total collected eggs in 2017 and 2018 seasons, respectively, and 2-14 parasitoids individuals (males and females) emerged from each pupa. The second species was Perilampus chrysopae (Crawford, 1914) (Hymenoptera, Perilampidae), which showed a parasitism rate of 5.13% and 23.81% of the total larvae collected in the two seasons, respectively, and one parasitoid individual (male or female) emerged from each pupa. Keywords: Chrysoperla carnea, Baryscapus chrysopae, Perilampus chrysopa, Telenomus acrobates, new record, Syria
Nabhan, M., Z.Sh。哈米斯和B.夏威夷。2023。叙利亚哈马省棉田绿草蛉寄生蜂的新记录及其生物学意义。植物保护学报,41(3):306-313。https://doi.org/10.22268/AJPP-41.3.306313本研究旨在鉴定金蝶各阶段的寄生蜂种类,并评价其对野外捕食者种群的影响。收集并鉴定了三种膜翅目寄生蜂。在叙利亚北部曾发现过寄生于卵上的背茧蜂(膜翅目:茧蜂科)。另外两种寄生在捕食者的幼虫-蛹阶段,本研究在叙利亚首次记录。第1种为茧蜂(Baryscapus chrysopae, Crawford, 1915)(膜翅目:茧蜂科),2017年和2018年茧蜂的寄生率分别为23.08%和28.75%,每蛹可孵化出2 ~ 14只雌雄寄生蜂。第2种为茧蜂(perampus chrysopae, Crawford, 1914)(膜翅目,茧蜂科),2个季节寄生蜂的寄生率分别为5.13%和23.81%,每个蛹均有1只雌雄寄生蜂。关键词:大黄菊,大黄菊,大黄菊,大黄菊,新记录,叙利亚
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引用次数: 0
The Mutating Effect of Microwave Irradiation on Spores and Crystal Protein Formation of Iraqi Bacillus thuringiensis kurstaki KS3 微波辐照对伊拉克苏云金芽孢杆菌KS3孢子和晶体蛋白形成的突变效应
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-01 DOI: 10.22268/ajpp-041.3.285291
Khlood Abedalelah Alkhafaji, Falah Hanash Nahar, Samira Auda Khlaywi, Mohammed Abedalrahem Abedalla, Ahmed Jaafar Feaath, Ameera Alwan Mezban, Sabreen Abedalhadi Saleh
Alkhafaji, K.A., F.H. Nahar, S.A. Khlaywi, M.A. Abedallah, A.J. Feaath, A.A. Mezban. and S.A. Saleh. 2023. The Mutating Effect of Microwave Irradiation on Spores and Crystal Protein Formation of Iraqi Bacillus thuringiensis kurstaki KS3. Arab Journal of Plant Protection, 41(3): 285-291. https://doi.org/10.22268/AJPP-041.3.285291 Bacillus thuringiensis is the most important biological control agent that is used in fields and stores against insect pests of agricultural importance. This research was conducted to study the effect of microwave radiation on sporogenesis and crystal protein production by the Iraqi bacterium B. thuringiensis KS3 strain. The bacterium was enriched by Lauria bertani broth (LB) pH 7.0 for spore formation. Spore suspension was microwave irradiated at 1000 Watt for 5, 10 and 15 seconds. Spore inactivation rate for each time period of microwave treatment was calculated and the macroscopic differences were examined. Crystal protein and spore production were estimated for irradiated and nonirradiated bacterial cultures. Viable spores in the control treatment was 3×108 viable spores/ml, and decreased after 5, 10, and 15 s of microwave treatment to 3×107 , 2×106 and 2×104 viable spores/ml, respectively. The reduction of spore viability reached to 49.263% 15 seconds after treatment. The appearance of colonies on the top of nutrient agar (NA) were almost similar with the control, with no differences in color, margin and surface of treated colonies following the three time periods treatment. Colonies with dense color, smaller in size and with straight margin appeared on congo red culture for all treatments in contrast to the control. Microscopic examination showed that treated bacilli were similar regarding their shape, diameter and arrangement, however,smaller bacterial cell size following 15 s irradiation treatment was observed. Control culture after 72 h started to form spores, whereas after 24 h, irradiated spores for 5, 10 and 15 s formed 75, 90 and 90% viable spores, respectively. Crystal protein reached the highest concentration after 72 h in control culture, whereas it varied based on treatment period. The production of viable spores from microwave irradiated isolates increased about one logarithmic cycle compared with the control culture of B. thuringiensis KS3.The peak of the UV spectrum of Crystal protein extracts was recognized at 255-280 nm and the peak of the curve indicated the protein concentration at a given wavelength. Differences were recognized in the UV light wavelength range of 220-235 nm. Keywords: Sporogenesis, Physical effect, Delta toxin.
Alkhafaji, k.a., F.H. Nahar, S.A. Khlaywi, M.A. Abedallah, A.J. Feaath, A.A. Mezban。和S.A. Saleh. 2023。微波辐照对伊拉克苏云金芽孢杆菌KS3孢子和晶体蛋白形成的突变效应植物保护学报,41(3):285-291。https://doi.org/10.22268/AJPP-041.3.285291苏云金芽孢杆菌是最重要的生物防治剂,用于田间和仓库防治重要的农业害虫。本试验研究了微波辐射对伊拉克苏云金芽孢杆菌KS3菌株孢子发生和晶体蛋白生产的影响。用pH 7.0的月桂肉汤(LB)培养细菌,培养孢子形成。将孢子悬浮液以1000瓦微波辐照5、10和15秒。计算了微波处理各时间段的孢子失活率,并考察了宏观差异。对辐照和非辐照细菌培养物的晶体蛋白和孢子产量进行了估计。对照处理的活孢子为3×108活孢子/ml,微波处理5 s、10 s和15 s后分别降至3×107、2×106和2×104活孢子/ml。处理15 s后,孢子活力降低49.263%。在营养琼脂(NA)上菌落的外观与对照基本相似,经过3个时间的处理,菌落的颜色、边缘和表面没有差异。与对照相比,刚果红培养中所有处理的菌落颜色浓密,大小较小,边缘直。镜检显示,处理后的细菌在形状、直径和排列上相似,但辐照15s后细菌细胞尺寸变小。对照培养72 h后开始形成孢子,照射5、10和15 s后孢子分别在24 h后形成75%、90%和90%的活孢子。晶体蛋白在对照培养72 h后达到最高浓度,但随处理时间的不同而变化。与对照培养的苏云金芽孢杆菌KS3相比,微波辐照分离株的活孢子产量增加了约一个对数周期。晶体蛋白提取物的紫外光谱峰在255 ~ 280 nm处,曲线峰表示该波长处的蛋白浓度。在220 ~ 235 nm紫外光波长范围内发现了差异。关键词:孢子发生,物理效应,德尔塔毒素
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引用次数: 0
A Taxonomic Study of Some Species of Hyperparasitoids Attacking the Primary Parasitoid Microterys nietneri of the Citricola Scale Insect, Coccus pseudomagnoliarum in Syria 叙利亚柠檬酸蚧虫pseudomagnoliarum的几种高寄生蜂攻击初级寄生蜂的分类学研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-01 DOI: 10.22268/ajpp-41.3.292305
Alaa Turki Saleh, Abdul Nabi Mohamad Basheer
Saleh, A.T. and A.M. Basheer. 2023. A Taxonomic Study of Some Species of Hyperparasitoids Attacking the Primary Parasitoid Microterys nietneri of the Citricola Scale Insect, Coccus pseudomagnoliarum in Syria. Arab Journal of Plant Protection, 41(3): 292-305. https://doi.org/10.22268/AJPP-41.3.292305 The citricola scale insect, Coccus pseudomagnoliarum, (Kuwana) (Hemiptera: Coccomorpha: Coccidae) is an economically important pest that occurs on citrus trees along the Syrian coast. The samples of host insects and infested plant parts were collected from April to June during 2016 and 2017 from two locations (Al-Sanobar, Dabba) in Lattakia Province and brought to the Biological Control Studies and Research Center (BCSRC), Faculty of Agriculture, Damascus University. Based on the information gathered, Microterys nietneri (Motschulsky) (Hymenoptera: Encyrtidae) may play an important role as a biological control agent of citricola scale insect, but high hyperparasitoids levels reduced M. nietneri efficiency as a citricola scale parasitoid. The objective of this study was to describe and identify naturally occurring hyperparasitoids. The following parasitoids were identified and reported for the first time in Syria: Cheiloneurus claviger (Thomson), Pachyneuron muscarum (Linnaeus), and Marietta leopardine (Motschulsky). Microscopic images were provided to illustrate the morphological and morphometric characteristics of these species. Keywords: Hyperparasitoids, Citricola scale, Microterys, Syria
萨利赫,A.T.和A.M.Basheer》2023。叙利亚柠檬酸蚧虫pseudomagnoliarum的几种高寄生蜂攻击初级寄生蜂的分类学研究。植物保护学报,41(3):292-305。https://doi.org/10.22268/AJPP-41.3.292305柑橘蚧虫,pseudomagnoliarum, (Kuwana)(半翅目:Coccomorpha: Coccidae)是一种重要的经济害虫,发生在叙利亚沿海的柑橘树上。于2016年4月至2017年6月在拉塔基亚省的两个地点(Al-Sanobar, Dabba)采集寄主昆虫和被侵染植物部位样本,并将样本送到大马士革大学农学院生物防治研究中心(BCSRC)。综上所述,尼氏微蜂(Microterys nietneri)(膜翅目:蜂科)可能作为柑桔蚧的生物防治剂发挥重要作用,但高寄生蜂水平降低了尼氏微蜂作为柑桔蚧的生物防治效果。本研究的目的是描述和鉴定自然发生的类超寄生虫。在叙利亚首次发现并报道的拟寄生虫有:Cheiloneurus claviger (Thomson)、Pachyneuron muscarum (Linnaeus)和Marietta leopardine (Motschulsky)。显微图像说明了这些物种的形态和形态计量特征。关键词:超寄生物,柑桔鳞片,微孢子虫,叙利亚
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引用次数: 0
Susceptibility Assessment of Two Tomato Hybrids Against Tuta absoluta Infestation Under Greenhouse Conditions 温室条件下两个番茄杂交种对绝对图塔线虫感染的敏感性评价
Q4 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.22268/ajpp-39.4.317322
A. Abdel-Razek, N. M. Abd El-Ghany, M. Gesraha, T. Elewa, A. Moussa
Abdel-Razek, A.S., N.M. Abd El-Ghany, M.A. Gesraha, T.A. Elewa and A. Moussa. 2021. Susceptibility Assessment of Two Tomato Hybrids Against Tuta absoluta Infestation Under Greenhouse Conditions. Arab Journal of Plant Protection, 39(4): 317-322. https://doi.org/10.22268/AJPP-39.4.317322 Tuta absoluta is a major insect pest which attack tomato plant varieties in Egypt. Several control attempts were carried out to avoid major crop losses by heavy application of chemical insecticides. The aim of the present study is to assess the susceptibility of infestation of T. absoluta of two tomato varieties (Shifa and Savera F1 hybrids) under greenhouse conditions. The tomato varieties were planted in two plantation periods at the district of Kom Hamada, El-Nubaria province, El-Behira Governorate. The susceptibility tests were done by random counting of leaf samples for the presence of T. absoluta mines and larvae. Both tomato varieties showed almost the same T. absoluta infestation level. Moreover, yield assessment was carried out for the two plantation periods by taking the average fruit weight yield (Kg/acre). The tomato yield results showed that Savera F1 hybrid tomato had higher yield compared to Shifa F1 hybrid variety, but such difference was not significant, However, the yield difference of both vireties between the two planting dates was significant. Keywords: Tomato, Tuta absoluta, susceptibility, leaf-mine, larvae, yield.
Abdel-Razek, a.s., N.M. Abd El-Ghany, M.A. Gesraha, T.A. Elewa和A. Moussa. 2021。温室条件下两种番茄杂交种对赤霉病的敏感性评价。植物保护学报,39(4):317-322。https://doi.org/10.22268/AJPP-39.4.317322绝对番茄螟是埃及番茄品种的主要害虫。为避免大量使用化学杀虫剂造成重大作物损失,进行了几次防治尝试。本研究旨在评价两个番茄品种(Shifa和Savera F1杂交品种)在温室条件下对赤霉病的易感性。这些番茄品种在El-Nubaria省和El-Behira省的Kom Hamada区分两个种植期种植。通过随机计数叶片样品,对绝对田鼠雷和幼虫的存在进行敏感性试验。两种番茄品种的侵染程度几乎相同。另外,以平均单果重产量(Kg/亩)对两个种植期进行产量评价。番茄产量结果表明,沙维拉F1杂交番茄产量高于世发F1杂交品种,但差异不显著,但两个品种在两个播种日期的产量差异显著。关键词:番茄,赤眼蜂,易感性,叶螨,幼虫,产量
{"title":"Susceptibility Assessment of Two Tomato Hybrids Against Tuta absoluta Infestation Under Greenhouse Conditions","authors":"A. Abdel-Razek, N. M. Abd El-Ghany, M. Gesraha, T. Elewa, A. Moussa","doi":"10.22268/ajpp-39.4.317322","DOIUrl":"https://doi.org/10.22268/ajpp-39.4.317322","url":null,"abstract":"Abdel-Razek, A.S., N.M. Abd El-Ghany, M.A. Gesraha, T.A. Elewa and A. Moussa. 2021. Susceptibility Assessment of Two Tomato Hybrids Against Tuta absoluta Infestation Under Greenhouse Conditions. Arab Journal of Plant Protection, 39(4): 317-322. https://doi.org/10.22268/AJPP-39.4.317322 Tuta absoluta is a major insect pest which attack tomato plant varieties in Egypt. Several control attempts were carried out to avoid major crop losses by heavy application of chemical insecticides. The aim of the present study is to assess the susceptibility of infestation of T. absoluta of two tomato varieties (Shifa and Savera F1 hybrids) under greenhouse conditions. The tomato varieties were planted in two plantation periods at the district of Kom Hamada, El-Nubaria province, El-Behira Governorate. The susceptibility tests were done by random counting of leaf samples for the presence of T. absoluta mines and larvae. Both tomato varieties showed almost the same T. absoluta infestation level. Moreover, yield assessment was carried out for the two plantation periods by taking the average fruit weight yield (Kg/acre). The tomato yield results showed that Savera F1 hybrid tomato had higher yield compared to Shifa F1 hybrid variety, but such difference was not significant, However, the yield difference of both vireties between the two planting dates was significant. Keywords: Tomato, Tuta absoluta, susceptibility, leaf-mine, larvae, yield.","PeriodicalId":37670,"journal":{"name":"Arab Journal of Plant Protection","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42762425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnosis and Inhibition of the Virulence Factor Phaseolenone of the Pathogenic Fungus Macrophomina phaseolina Using Some Chemical and Biological Methods 几种化学和生物学方法诊断和抑制菜绿巨噬菌毒力因子Phaseolenone
Q4 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.22268/ajpp-039.4.252256
Aouf Al Jbory, A. Hasan
Al-Jbory, A.A.A. and A.A Hasan. 2021. Diagnosis and Inhibition of the Virulence Factor Phaseolenone of the Pathogenic Fungus Macrophomina phaseolina Using Some Chemical and Biological Methods. Arab Journal of Plant Protection, 39(4): 252-256. https://doi.org/10.22268/AJPP-039.4.252256 Results obtained in this study confirmed the efficacy of some chemical and biological factors in reducing the concentration of Phaseolenone toxin produced by the fungal pathogen Macrophomina phaseolina grown on potato dextrose broth medium. The toxin concentrations were measured in different treatments using high performance liquid chromatography (HPLC). The citric acid treatment at 5% concentration surpassed the other treatments (lactic acid, acetic acid, SDS salts, EDTA, bacterial filtrates of Pseudomonas fluorescens and Bacillus subtilis). Citric acid treatment reduced by 5% the toxin concentration to 1.74 microgram/ml compared to 6.94 microgram/ml for the control treatment. This reduction in Phaseolenone concentration was also noticed through the reduction of the area under the curve which amounted to 1.684% for the citric acid treatment compared with 6.814% for the control treatment. Keywords: Phaseolenone, Macrophomina Phaseolina, charcoal rot, mycotoxin.
Al Jbory、A.A.A.和A.A.Hasan。2021.使用一些化学和生物学方法诊断和抑制致病真菌大磷虾的毒力因子Phaseolenone。《阿拉伯植物保护杂志》,39(4):252-256。https://doi.org/10.22268/AJPP-039.4.252256本研究的结果证实了一些化学和生物因素在降低由生长在马铃薯葡萄糖肉汤培养基上的真菌病原体Phaphomina phaseolina产生的Phaseolenone毒素浓度方面的功效。使用高效液相色谱法(HPLC)测量不同处理中的毒素浓度。5%浓度的柠檬酸处理超过了其他处理(乳酸、乙酸、SDS盐、EDTA、荧光假单胞菌和枯草芽孢杆菌的细菌滤液)。柠檬酸处理将毒素浓度降低了5%,达到1.74微克/毫升,而对照处理为6.94微克/ml。Phaseolenone浓度的降低也通过曲线下面积的减少而被注意到,与对照处理的6.814%相比,柠檬酸处理的曲线下面积为1.684%。关键词:菜豆酮,菜豆大孔菌,炭腐病,真菌毒素。
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引用次数: 0
Effect of Olive Mill Wastewater of in the Control of Olive Verticillium Wilt Caused by Verticillium dahlia Kleb 橄榄厂废水防治大丽花黄萎的效果
Q4 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.22268/ajpp-39.4.273280
Khiyam Muhrez, M. Tawil, Bassema Barhoum
Muhrez, K.A., M.Z. Tawil and B. Barhoum. 2021. Effect of Olive Mill Wastewater of in the Control of Olive Verticillium Wilt Caused by Verticillium dahlia Kleb. Arab Journal of Plant Protection, 39(4): 273-280. https://doi.org/10.22268/AJPP-39.4.273280 This research aimed to evaluate the application of olive mill wastewater in controlling Verticillium dahliae the causal agent of olive wilt disease on olive trees. This study was carried out on 2 years old olive local variety (Khderi) plants planted in pots after it has been artificial inoculated with V. dahliae, and severity was recorded within 90 days. Olive mill wastewater (omww) efficacy was estimated at the end of the experiment. Severity was increased from 8.89% before treatment to 28.89% 60 days after treatment, and decreased to 13.33% 90 days after treatment with omww 450 ml/l. Disease severity increased from 11.11% before treatment to 37.78% 60 days after treatment and decreased to 35.56% 90 days after treatment with omww 300 ml/l. Whereas, disease severity increased from 17.78% before treatment to 40% 90 days after treatment with azoxystrobin compared to the inoculated control where disease severity increased from 13.33% before treatment to 100% 90 days after treatment. Omww 450 ml/l had the highest efficacy of 80% followed by azoxystrobin 70%, and omww 300 ml/l 57.33%. It can be concluded that olive mill wastewater has a potential application as a bio-fungicide for the control of olive verticillium wilt disease. Keywords: Olive mill waste waters, Verticillium dahlia, olive, Fungicide.
Muhrez, k.a., M.Z. Tawil和B. Barhoum, 2021。橄榄厂废水防治大丽花黄萎病的效果。植物保护学报,39(4):273-280。https://doi.org/10.22268/AJPP-39.4.273280本研究旨在评价橄榄厂废水在防治橄榄枯萎病病原菌大丽花黄萎病中的应用。本研究以2年生橄榄地方品种(Khderi)为研究对象,经大丽花弧菌人工接种后盆栽种植,90 d内记录严重程度。在试验结束时对橄榄厂废水(omww)的处理效果进行了评估。治疗前患者的严重程度为8.89%,治疗后60天为28.89%,治疗后90天为13.33%。剂量为300 ml/l的omww治疗后,疾病严重程度从治疗前的11.11%上升至治疗后60天的37.78%,90天下降至35.56%。然而,与接种对照组相比,接种azoxystrobin后90天疾病严重程度从治疗前的17.78%增加到40%,而接种对照组的疾病严重程度从治疗前的13.33%增加到治疗后90天的100%。Omww 450 ml/l的效价最高,为80%,其次为唑虫胺酯70%,Omww 300 ml/l的效价最高,为57.33%。由此可见,橄榄厂废水具有作为生物杀菌剂防治橄榄黄萎病的潜力。关键词:橄榄厂废水;大丽花黄萎病菌;橄榄;
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引用次数: 0
Induction of Systemic Resistance in Tomato Plants Against Tomato yellow leaf curl virus in Protected Cultivation Using a Local Bacterial Isolate of Bacillus subtilis 枯草芽孢杆菌地方分离株在设施栽培中诱导番茄对番茄黄叶卷曲病毒的系统抗性
Q4 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.22268/ajpp-039.4.289295
H. Ghanem, Insaf Akel, Quosai Al-Rhayeh, I. Ismail
Ghanem, H.M., E.H. Akel, Q.A. Al-Rhayeh and I.D. Ismail. 2021. Induction of Systemic Resistance in Tomato Plants Against Tomato yellow leaf curl virus in Protected Cultivation Using a Local Bacterial Isolate of Bacillus subtilis. Arab Journal of Plant Protection, 39(4): 289-295. https://doi.org/10.22268/AJPP-039.4.289295 This study was conducted at the Agricultural Scientific Research Center in Lattakia Governorate in a plastic house during the 2020/2021 growing season to evaluate the efficiency of the native bacterial isolate B.Ra.217 of Bacillus subtilis, in reducing infection of tomato plants with Tomato yellow leaf curl virus (TYLCV), by treating tomato seeds and later watering the seedlings with a suspension of the tested bacteria at a concentration of 1x109 /ml, and then measuring incidence (%) and severity of virus infection, and estimating peroxidase enzyme activity. The results showed that 30 days after inoculation with tomato leaf curl virus, a decrease in virus infection rate and severity in virus-infected and bacteria-treated plants compared with virus-infected and untreated control plants was observed. The reduction in disease incidence and severity of infection reached 26.67% and 34.28%, respectively, with significant differences between the treatments. In addition, the activity of peroxidase enzyme 7 days after infection with the virus showed an increase in plants treated with bacteria only (0.1342 μmol/mg) and those treated with bacteria and virus-infected (0.0913 μmol/mg), compared with the healthy control plants (0.0958 μmol/mg), and virus-infected and untreated with bacteria (0.0570 µmol/mg). The results also showed 15 days after infection that the enzyme activity was higher in plants treated with bacteria only (0.1592 µmol/mg) compared with the healthy control (0.1415 µmol/mg) with significant differences, and also the differences were significant with the untreated infected plants (0.1002 µmol/mg), and with inoculated and treated plants (0.1372 µmol/mg). Thus, this bacterial isolate may have an important applied role in enhancing tomato plant resistance to the virus and consequently reducing its damage. Keywords: Bacillus subtilis B.Ra.217, TYLCV, peroxidase, incidence, severity of infestation, tomato plant
Ghanem,H.M.,E.H.Akel,Q.A.Al Rhayeh和I.D.Ismail。2021.使用枯草芽孢杆菌的本地细菌分离物在保护性栽培中诱导番茄植株对番茄黄叶卷曲病毒的系统抗性。《阿拉伯植物保护杂志》,39(4):289-295。https://doi.org/10.22268/AJPP-039.4.289295这项研究是在拉塔基亚省农业科学研究中心2020/2021生长季节的一个塑料屋里进行的,以评估枯草芽孢杆菌的本土细菌分离株B.Ra.217在减少番茄黄叶卷曲病毒(TYLCV)感染番茄植株方面的效率,通过处理番茄种子,然后用浓度为1x109/ml的测试细菌悬浮液浇灌幼苗,然后测量病毒感染的发生率(%)和严重程度,并估计过氧化物酶活性。结果表明,接种番茄卷叶病毒30天后,与感染病毒和未处理的对照植物相比,感染病毒和细菌处理的植物的病毒感染率和严重程度有所下降。疾病发生率和感染严重程度的降低分别达到26.67%和34.28%,两种治疗之间存在显著差异。此外,与健康对照植物(0.0958μmol/mg)、病毒感染和未经细菌处理的植物(0.0570μmol/mg。结果还显示,感染后15天,仅用细菌处理的植物的酶活性(0.1592µmol/mg)高于健康对照(0.1415µmol/mg。因此,这种细菌分离物可能在增强番茄植物对病毒的抵抗力从而减少其危害方面具有重要的应用作用。关键词:枯草芽孢杆菌B.Ra.217,TYLCV,过氧化物酶,发病率,虫害严重程度,番茄植株
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引用次数: 0
The Potency of Six Medicinal Plant Extracts Against the Stored Grain Insect Pest Sitophilus granarius L. 六种药用植物提取物对储粮害虫象虫的药效研究。
Q4 Agricultural and Biological Sciences Pub Date : 2021-12-01 DOI: 10.22268/ajpp-039.4.323328
N. Jawalkar, S. Zambare, Mohamed Izzat Al Ghannoum
Jawalkar, N.B., S.P. Zambare and M.I. Al Ghannoum. 2021. The Potency of Six Medicinal Plant Extracts Against the Stored Grain Insect Pest Sitophilus granarius L. Arab Journal of Plant Protection, 39(4): 323-328. https://doi.org/10.22268/AJPP-039.4.323328 This study was conducted on six medicinal plants viz., Vitex negundo (leaves), Xanthium strumarium, Caesalpinia bonduc, Mucuna pruriens, Moringa oleifera (seed kernels), Tagetes erecta (petals) for their bio-insecticidal activity. The powders of various parts of plants were extracted using the MARS6 microwave acid digestion system. Three different concentrations (20, 30, and 40%) of plant extracts were tested against granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae) for their bio-insecticidal activities under laboratory conditions. The results of statistical analysis showed a good performance of all plant extracts, especially at the high concentrations of the extracts, where they showed different levels of insect mortality and their developmental rate was also reduced leading to significant reduction in insect numbers. The mortality rate ranged was 0-100% with S. granarius adults (p < 0.01). There was significant correlation (R=1) between mortality rate and the concentration of plant extracts. However, the extract of Mucuna pruriens with solvents (Acetone + Petroleum Ether) showed the highest mortality rate of 100% at the three concentrations used (p < 0.01), whereas, the lowest average mortality of 43.3% (p < 0.01) was observed with Xanthium strumarium and Vitex negundo extracts with solvents (Methanol + n-Hexane) compared with 0% mortality in the control. All plant extracts have revealed insecticidal as well as propitious protective effect on grains, and it can be selected as effective control treatment after proper dose formulation to prevent weevil infestation in stored grains. Keywords: Bioinsecticide, stored grains, Sitophilus granarius, mortality, medicinal plants, MARS 6.
Jawalkar,N.B.,S.P.Zambare和M.I.Al-Ghannoum。2021.六种药用植物提取物对抗储粮害虫石榴西氏体的效力。阿拉伯植物保护杂志,39(4):323-328。https://doi.org/10.22268/AJPP-039.4.323328本研究对六种药用植物,即黄荆(叶)、苍耳子(Xanthium strumarium)、山杨(Caesalpia bonduc)、痒痒木(Mucuna stricens)、辣木(种子仁)、万寿菊(花瓣)的生物杀虫活性进行了研究。利用MARS6微波酸消化系统提取植物各部位的粉末。在实验室条件下,测试了三种不同浓度(20%、30%和40%)的植物提取物对石榴象甲(鞘翅目:Curculionidae)的生物杀虫活性。统计分析结果显示,所有植物提取物都表现良好,尤其是在高浓度提取物下,它们表现出不同程度的昆虫死亡率,其发育率也降低,导致昆虫数量显著减少。石榴子成虫的死亡率在0-100%之间(p<0.01)。死亡率与植物提取物浓度之间存在显著相关性(R=1)。然而,在使用的三种浓度下,用溶剂(丙酮+石油醚)提取的瘙痒性粘液的死亡率最高,为100%(p<0.01),而用溶剂(甲醇+正己烷)提取的苍耳和黄荆的平均死亡率最低,为43.3%(p<0.01)。所有植物提取物都显示出杀虫和良好的粮食保护作用,在适当的剂量配方后,可以选择它作为有效的防治处理,以防止储存粮食中的象甲侵扰。关键词:生物杀虫剂,储粮,谷粒鼠,死亡率,药用植物,MARS 6。
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引用次数: 0
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Arab Journal of Plant Protection
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