Pub Date : 2018-11-07DOI: 10.5584/jiomics.v8i4.221
Feiyu Yang, Yun Zou, C. Ni, Chenggong Zhang, Fenjin Sun, Xiaoliang Yuan, Rong Wang, Wenbin Liu
{"title":"An Automated Magnetic Dispersive Solid-phase Extraction Method for Detection of Cocaine and its Metabolites in Human Urine","authors":"Feiyu Yang, Yun Zou, C. Ni, Chenggong Zhang, Fenjin Sun, Xiaoliang Yuan, Rong Wang, Wenbin Liu","doi":"10.5584/jiomics.v8i4.221","DOIUrl":"https://doi.org/10.5584/jiomics.v8i4.221","url":null,"abstract":"","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45270582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-22DOI: 10.5584/JIOMICS.V8I3.236
I. Aquila, M. Sacco, S. Gratteri, Roberto Raffaele, P. Ricci
Post Mortem Interval (PMI) estimation is one of the most important questions the forensic pathologist must answer. To date, it is not possible to establish exactly the hour of death, but only to calculate a period (PMI), during which death most likely occurred. In the forensic field, several laboratory methods can be used to perform this calculation more accurately. However, there is still no biomarker that is universally validated and accepted by the forensic community. In the literature, researches about the application of proteomics for forensic purposes are on the increase. Proteomics is a branch of molecular biology that allows the systematic identification of the proteome from a quantitative and qualitative point of view. Below, we propose the operating model of an experimental study currently underway at the Department of Legal Medicine of the University of Catanzaro. The model is based on taking of peripheral blood samples from patients who died at the Intensive Care Unit (AO “Mater Domini” of Catanzaro). The proposed operating model has several advantages including the evaluation, for the first time, of human biological samples from the exact moment of death. The analysis would allow to identify new potential biomarkers expressed in peripheral blood and validate the forensic application of markers already known in the literature. The knowledge of the exact moment of death (time 0) would allow us to evaluate the proteomic profile more accurately on the human model, overstepping the limits of some extrinsic variables evidenced in the literature. Normal 0 14 false false false IT X-NONE X-NONE
{"title":"THE FORENSIC APPLICATION OF PROTEOMICS FOR THE STUDY OF THE TIME OF DEATH: AN OPERATIVE EXPERIMENTAL MODEL FOR PMI ESTIMATION","authors":"I. Aquila, M. Sacco, S. Gratteri, Roberto Raffaele, P. Ricci","doi":"10.5584/JIOMICS.V8I3.236","DOIUrl":"https://doi.org/10.5584/JIOMICS.V8I3.236","url":null,"abstract":"Post Mortem Interval (PMI) estimation is one of the most important questions the forensic pathologist must answer. To date, it is not possible to establish exactly the hour of death, but only to calculate a period (PMI), during which death most likely occurred. In the forensic field, several laboratory methods can be used to perform this calculation more accurately. However, there is still no biomarker that is universally validated and accepted by the forensic community. In the literature, researches about the application of proteomics for forensic purposes are on the increase. Proteomics is a branch of molecular biology that allows the systematic identification of the proteome from a quantitative and qualitative point of view. Below, we propose the operating model of an experimental study currently underway at the Department of Legal Medicine of the University of Catanzaro. The model is based on taking of peripheral blood samples from patients who died at the Intensive Care Unit (AO “Mater Domini” of Catanzaro). The proposed operating model has several advantages including the evaluation, for the first time, of human biological samples from the exact moment of death. The analysis would allow to identify new potential biomarkers expressed in peripheral blood and validate the forensic application of markers already known in the literature. The knowledge of the exact moment of death (time 0) would allow us to evaluate the proteomic profile more accurately on the human model, overstepping the limits of some extrinsic variables evidenced in the literature. Normal 0 14 false false false IT X-NONE X-NONE","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5584/JIOMICS.V8I3.236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49495589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-30DOI: 10.5584/jiomics.v8i2.254
José Capelo
{"title":"VOL 8, No 2: SPECIAL ISSUE No 1 (2018)","authors":"José Capelo","doi":"10.5584/jiomics.v8i2.254","DOIUrl":"https://doi.org/10.5584/jiomics.v8i2.254","url":null,"abstract":"","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43269253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.5584/JIOMICS.V8I1.204
O. Alzate
Analysis of the proteome of any Bacillus thuringiensis strain should provide important information about mechanisms of infection, interactions with host organisms, and very importantly, about the molecular mechanisms by which the bacterium is able to survive under non-favorable conditions. In order to address these important issues, we analyzed the proteome of the crystal-forming Bacillus thuringiensis strain BGSC-4AW1 (var. andalousiensis ). This proteomic analysis reveals the presence of important proteins for cell survival and cell proliferation associated with the exosporium/coat/crystal complexes. Although, at the present time, we cannot discriminate among the specific sub proteomes associated with these developmental stages, it is clear that this information should be useful for mapping the cellular mechanisms involved in cell survival and adaptation to detrimental environmental conditions. It is also clear that the proteomic analysis offers a wider window into the potential biotechnological applications of Bt .
{"title":"Quantitative proteomic analysis of the Bacillus thuringiensis BGSC-4AW1 strain (serovar andalousiensis)","authors":"O. Alzate","doi":"10.5584/JIOMICS.V8I1.204","DOIUrl":"https://doi.org/10.5584/JIOMICS.V8I1.204","url":null,"abstract":"Analysis of the proteome of any Bacillus thuringiensis strain should provide important information about mechanisms of infection, interactions with host organisms, and very importantly, about the molecular mechanisms by which the bacterium is able to survive under non-favorable conditions. In order to address these important issues, we analyzed the proteome of the crystal-forming Bacillus thuringiensis strain BGSC-4AW1 (var. andalousiensis ). This proteomic analysis reveals the presence of important proteins for cell survival and cell proliferation associated with the exosporium/coat/crystal complexes. Although, at the present time, we cannot discriminate among the specific sub proteomes associated with these developmental stages, it is clear that this information should be useful for mapping the cellular mechanisms involved in cell survival and adaptation to detrimental environmental conditions. It is also clear that the proteomic analysis offers a wider window into the potential biotechnological applications of Bt .","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5584/JIOMICS.V8I1.204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45470013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-01DOI: 10.5584/jiomics.v8i1.240
Martha Fabiola Garcia Alvarez
The purpose of the multidisciplinary investigation was to analyze the participation of violence in homicidal women (Ferro, 2012:121-129), from the criminological aspects; By means of the field work done at the Preventive and Female Rehabilitation Center of Puente Grande, Jalisco, Mexico (2008-2014).In methodology, criminological, sociological and law research methods were handled; with interviews with female inmates accused of homicide; including their life history, using a sample of 25%, and analyzing the psychobiological and socio-legal variables.In the discussion and results, there is influence of psychobiological and social aspects in the violent behavior of women, and who has had a life history impregnated with violence, mainly by the family, patron (Lima, 1991) who repeat with children and/or against those who come to affect them, feeling threatened in their integrity. In this complex analysis there are several indicators and external and internal agents that encourage women to apply violence. This entails an interfactorial and multifactorial relationship between related causalities, as in the study of criminal Victimology (Marchiori, 2000).It is concluded that violent acts carried out by homicides come from a combination of multifactorial aspects, in addition to the change of role from victim to victim, in 90% of thecases, which brings with it psychological and social problems in the behavior of The homicidas, being added in some of them the mental illnesses and with a high degree of aggressiveness.
{"title":"Indicative of Violence in Homicidal Women","authors":"Martha Fabiola Garcia Alvarez","doi":"10.5584/jiomics.v8i1.240","DOIUrl":"https://doi.org/10.5584/jiomics.v8i1.240","url":null,"abstract":"The purpose of the multidisciplinary investigation was to analyze the participation of violence in homicidal women (Ferro, 2012:121-129), from the criminological aspects; By means of the field work done at the Preventive and Female Rehabilitation Center of Puente Grande, Jalisco, Mexico (2008-2014).In methodology, criminological, sociological and law research methods were handled; with interviews with female inmates accused of homicide; including their life history, using a sample of 25%, and analyzing the psychobiological and socio-legal variables.In the discussion and results, there is influence of psychobiological and social aspects in the violent behavior of women, and who has had a life history impregnated with violence, mainly by the family, patron (Lima, 1991) who repeat with children and/or against those who come to affect them, feeling threatened in their integrity. In this complex analysis there are several indicators and external and internal agents that encourage women to apply violence. This entails an interfactorial and multifactorial relationship between related causalities, as in the study of criminal Victimology (Marchiori, 2000).It is concluded that violent acts carried out by homicides come from a combination of multifactorial aspects, in addition to the change of role from victim to victim, in 90% of thecases, which brings with it psychological and social problems in the behavior of The homicidas, being added in some of them the mental illnesses and with a high degree of aggressiveness.","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5584/jiomics.v8i1.240","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70838972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-30DOI: 10.5584/JIOMICS.V8I1.226
Matteo Nioi, P. Napoli, F. Paribello, R. Demontis, F. Giorgio, E. Porru, M. Fossarello, E. d’Aloja
PURPOSE: The aim of this study was to describe by means of a portable Optical Coherence Tomography (OCT) the postmortem ocular findings in two cases of forensic interest. CASE 1. A 41-year-old Caucasian man, dead from a gunshot in the head, was found inside his car. Time of death was precisely assessed from the testimony of eye witnesses. The body was transported at the Morgue of Medico-Legal Institute at the University of Cagliari for autopsy. OCT scans of cornea, anterior chamber and retina were performed at the 6th, 12th and 24th hour postmortem without change eyelid opening status. Corneal examination showed a progressive tendency of tissue to thickening. From a morphological point of view, we also observed a progressive formation of waves in the posterior stroma and in the endothelium [we decided to call this phenomenon, described by our group for first time, Nioi-Napoli sign (NN sign)]. An ongoing modification in reflectivity between anterior (hyperreflective) and posterior (hypo-reflective) segment of the corneal stroma was detecetd and a progressive decrease in amplitude of anterior chamber, mainly related to changes in tissue curvature. The retinal tissue showed since the first scan an increasing trend of retinal oedema togheter with a diffuse vasal depletion. It was also detachable the formation of a scleral tache noir. CASE 2. A 42-year-old woman, dead from myocardial infarction, underwent an autopsy at the Medico-Legal Institute at University of Cagliari (as suspected case of medical malpractice). Exact time of death was deduced by certificate of death drawn up by the emergency medical team. Scans of cornea, retina and anterior chamber were executed at the 24th, 36th and 48th hour postmortem. From the first scan an enhancement of corneal thickness, if compared to the physiological in vivo ranges, was detected; a change in corneal curvature was observed while no images form the retinal tissue was achieved. Late scans evidenced a progressive corneal endothelial exfoliation, and an enhancement of posterior stroma waving. CONCLUSION Portable OCT may be an useful device to observe and to record postmortem ocular changes. 1,2,3 Its use could represent an important tool to study the early and the late modification of ocular tissues, with a special interest in the forensic scenario as post mortem interval estimation (PMI estimation) and in ophthalmology (viability of cornea for transplantation purposes).
{"title":"Use of Optical Coherence Tomography on detection of postmortem Ocular findings: pilot data from two cases.","authors":"Matteo Nioi, P. Napoli, F. Paribello, R. Demontis, F. Giorgio, E. Porru, M. Fossarello, E. d’Aloja","doi":"10.5584/JIOMICS.V8I1.226","DOIUrl":"https://doi.org/10.5584/JIOMICS.V8I1.226","url":null,"abstract":"PURPOSE: The aim of this study was to describe by means of a portable Optical Coherence Tomography (OCT) the postmortem ocular findings in two cases of forensic interest. CASE 1. A 41-year-old Caucasian man, dead from a gunshot in the head, was found inside his car. Time of death was precisely assessed from the testimony of eye witnesses. The body was transported at the Morgue of Medico-Legal Institute at the University of Cagliari for autopsy. OCT scans of cornea, anterior chamber and retina were performed at the 6th, 12th and 24th hour postmortem without change eyelid opening status. Corneal examination showed a progressive tendency of tissue to thickening. From a morphological point of view, we also observed a progressive formation of waves in the posterior stroma and in the endothelium [we decided to call this phenomenon, described by our group for first time, Nioi-Napoli sign (NN sign)]. An ongoing modification in reflectivity between anterior (hyperreflective) and posterior (hypo-reflective) segment of the corneal stroma was detecetd and a progressive decrease in amplitude of anterior chamber, mainly related to changes in tissue curvature. The retinal tissue showed since the first scan an increasing trend of retinal oedema togheter with a diffuse vasal depletion. It was also detachable the formation of a scleral tache noir. CASE 2. A 42-year-old woman, dead from myocardial infarction, underwent an autopsy at the Medico-Legal Institute at University of Cagliari (as suspected case of medical malpractice). Exact time of death was deduced by certificate of death drawn up by the emergency medical team. Scans of cornea, retina and anterior chamber were executed at the 24th, 36th and 48th hour postmortem. From the first scan an enhancement of corneal thickness, if compared to the physiological in vivo ranges, was detected; a change in corneal curvature was observed while no images form the retinal tissue was achieved. Late scans evidenced a progressive corneal endothelial exfoliation, and an enhancement of posterior stroma waving. CONCLUSION Portable OCT may be an useful device to observe and to record postmortem ocular changes. 1,2,3 Its use could represent an important tool to study the early and the late modification of ocular tissues, with a special interest in the forensic scenario as post mortem interval estimation (PMI estimation) and in ophthalmology (viability of cornea for transplantation purposes).","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5584/JIOMICS.V8I1.226","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45912434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-07DOI: 10.5584/jiomics.v8i1.219
Juan Bernardo Montoya-Mogollón, Sonia Maria Troitiño Rodriguez
This paper aims at the application of Digital Diplomatic and Digital Forensics Science in digital-born records, in order to guarantee its authenticity in institutional routines and processes, but also as a source of proof in possible legal and juridical scenarios. In view of the vast and fruitful discussions focused on the complexity of the digital records in Archival Science and Information Science (I.S.), there is a gap to manage and preserve the digital records, keeping them reliable, accurate and authentic in systems that have the same conditions. This gap makes it impossible to preserve records in the long term due, firstly, to the fragility of the systems where they are stored and, secondly, to the constant risks of obsolescence of hardware and software that occur on a day by day. Digital Diplomatic Science assists this process by determining the form and content of the record to evidence its legal-diplomatic authenticity and establish its historical value. Digital Forensic Science, in turn, provides support for the chain of custody to remain intact, regardless of the medium on which the digital record is fixed. The following questions is raised: Is it possible to apply the practice of law and the Digital Forensics in the area of the Archival Science and information science, guaranteeing the authenticity of the born-digital record. In addition, in which way does the junction between Digital Diplomatic Science and Digital Forensic Science guarantee the preservation and preservation of the born-digital records. To answer such questions, this research proposes to link five areas of knowledge following the scientific experiences conducted at the University of British Columbia in Canada by the InterPares (International Research on Permanent Authentic Records) group. Areas of knowledge that are offering interesting results for the preservation not only of the record, but also of the social memory: 1.Digital Forensics: discipline that joint Forensics Science with Computer Science to analyze the digital evidence. 2. Diplomatics Science: Science to analyze the form and structure to the records. 3. Archival Science: Science to involves the whole of the principles, policies, strategies, and activities designed to ensure the physical and technological stabilization of records for the purpose of extending indefinitely their life and protecting the accuracy and authenticity of and maintaining the accessibility to their intellectually content [1] . 4. Information Science: Science to analyze the information in different contexts. The research will carried out in a theoretical and qualitative way, taking into account the literature shared in the site Digital Records Forensics Project, coordinated by the author Luciana Duranti of the InterPares group, and the bibliography produced in the country of the areas of knowledge already referenced. The results obtained will contribute to the realization of alternative researches in document conservation within the A
{"title":"The Diplomatic and Digital Forensic Science in Born-Digital Records: The Quest for Authenticity","authors":"Juan Bernardo Montoya-Mogollón, Sonia Maria Troitiño Rodriguez","doi":"10.5584/jiomics.v8i1.219","DOIUrl":"https://doi.org/10.5584/jiomics.v8i1.219","url":null,"abstract":"This paper aims at the application of Digital Diplomatic and Digital Forensics Science in digital-born records, in order to guarantee its authenticity in institutional routines and processes, but also as a source of proof in possible legal and juridical scenarios. In view of the vast and fruitful discussions focused on the complexity of the digital records in Archival Science and Information Science (I.S.), there is a gap to manage and preserve the digital records, keeping them reliable, accurate and authentic in systems that have the same conditions. This gap makes it impossible to preserve records in the long term due, firstly, to the fragility of the systems where they are stored and, secondly, to the constant risks of obsolescence of hardware and software that occur on a day by day. Digital Diplomatic Science assists this process by determining the form and content of the record to evidence its legal-diplomatic authenticity and establish its historical value. Digital Forensic Science, in turn, provides support for the chain of custody to remain intact, regardless of the medium on which the digital record is fixed. The following questions is raised: Is it possible to apply the practice of law and the Digital Forensics in the area of the Archival Science and information science, guaranteeing the authenticity of the born-digital record. In addition, in which way does the junction between Digital Diplomatic Science and Digital Forensic Science guarantee the preservation and preservation of the born-digital records. To answer such questions, this research proposes to link five areas of knowledge following the scientific experiences conducted at the University of British Columbia in Canada by the InterPares (International Research on Permanent Authentic Records) group. Areas of knowledge that are offering interesting results for the preservation not only of the record, but also of the social memory: 1.Digital Forensics: discipline that joint Forensics Science with Computer Science to analyze the digital evidence. 2. Diplomatics Science: Science to analyze the form and structure to the records. 3. Archival Science: Science to involves the whole of the principles, policies, strategies, and activities designed to ensure the physical and technological stabilization of records for the purpose of extending indefinitely their life and protecting the accuracy and authenticity of and maintaining the accessibility to their intellectually content [1] . 4. Information Science: Science to analyze the information in different contexts. The research will carried out in a theoretical and qualitative way, taking into account the literature shared in the site Digital Records Forensics Project, coordinated by the author Luciana Duranti of the InterPares group, and the bibliography produced in the country of the areas of knowledge already referenced. The results obtained will contribute to the realization of alternative researches in document conservation within the A","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5584/jiomics.v8i1.219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43414648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01Epub Date: 2018-04-16DOI: 10.5584/jiomics.v8i1.216
V Lopes de Andrade, M L Mateus, M Aschner, Ap Marreilha Dos Santos
Chronic occupational exposures to low levels of metal mixtures necessitates biomonitoring of exposed workers. However, a single biomarker (BM) is rarely sufficient to ascertain the exposure of an individual to a complex mixture, with multiparameter analysis of the same sample considered recently as a preferred approach. Porphyrins are formed as intermediates of heme biosynthesis and different metals can exert their effects at different points of this metabolic pathway, leading to changed urinary porphyrins excretion profiles. The aim of this work was to develop a model that could serve to identify, on an individual basis, multiple metal exposure resulting from mining work, by using urinary porphyrin profiles. Urine samples of workers were obtained from a Portuguese mining company and a non-occupationally exposed group was used as control. The levels of uro-, hepta-, hexa-, penta-, copro- and protoporphyrins were determined by HPLC. It was observed that only heptaporphyrin levels in miners were significantly (p<0.05) different from controls. However, when the concentrations of all porphyrins were combined by binary logistic regression, their ability to discriminate between miners and controls was higher than each one of the porphyrins alone, as indicated by a greater curve' area under a ROC curve. Moreover, when the combined porphyrins were used to calculate the probability of each subject fit in the occupationally exposed group, 83% of 47 individuals were correctly identified with respect to their type of exposure. These results suggest that the integration of the urinary porphyrin profile is a promising tool for the detection of subjects exhibiting biochemical modifications due to occupational exposure to metals in mines.
{"title":"Assessment of occupational exposures to multiple metals with urinary porphyrin profiles.","authors":"V Lopes de Andrade, M L Mateus, M Aschner, Ap Marreilha Dos Santos","doi":"10.5584/jiomics.v8i1.216","DOIUrl":"https://doi.org/10.5584/jiomics.v8i1.216","url":null,"abstract":"<p><p>Chronic occupational exposures to low levels of metal mixtures necessitates biomonitoring of exposed workers. However, a single biomarker (BM) is rarely sufficient to ascertain the exposure of an individual to a complex mixture, with multiparameter analysis of the same sample considered recently as a preferred approach. Porphyrins are formed as intermediates of heme biosynthesis and different metals can exert their effects at different points of this metabolic pathway, leading to changed urinary porphyrins excretion profiles. The aim of this work was to develop a model that could serve to identify, on an individual basis, multiple metal exposure resulting from mining work, by using urinary porphyrin profiles. Urine samples of workers were obtained from a Portuguese mining company and a non-occupationally exposed group was used as control. The levels of uro-, hepta-, hexa-, penta-, copro- and protoporphyrins were determined by HPLC. It was observed that only heptaporphyrin levels in miners were significantly (p<0.05) different from controls. However, when the concentrations of all porphyrins were combined by binary logistic regression, their ability to discriminate between miners and controls was higher than each one of the porphyrins alone, as indicated by a greater curve' area under a ROC curve. Moreover, when the combined porphyrins were used to calculate the probability of each subject fit in the occupationally exposed group, 83% of 47 individuals were correctly identified with respect to their type of exposure. These results suggest that the integration of the urinary porphyrin profile is a promising tool for the detection of subjects exhibiting biochemical modifications due to occupational exposure to metals in mines.</p>","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5584/jiomics.v8i1.216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36926494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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