Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.05.006
Armughan S. Khan MBBS, Anam Khan MD, Zarrin Hossein-Zadeh MD, Lawra Murray CT, MS (ASCP), Karen Chau MBA, CT(ASCP), Atif Khan MD, Xinmin Zhang MD, Rubina S. Cocker MD
Introduction
Primary vitreoretinal lymphoma (PVRL) is a rare, aggressive, and intraocular non-Hodgkin lymphoma, typically manifesting as diffuse large B-cell lymphoma (95%). Vitreous fluid cytology is the gold standard for diagnosis; however, its utility is limited by poor preservation and low cellularity. Recent studies indicate that myeloid differentation primary response protein 88 (MYD88) mutation analysis is more sensitive and accurate on low-cellularity or poorly preserved samples. The incidence of PVRL has reportedly tripled with an annual average of ∼50 cases in the United States. Delayed diagnosis can lead to mortality within 2 years, underscoring the need for improved diagnostic methods.
Materials and methods
We conducted a 5-year retrospective study of vitreous samples from 3 tertiary centers. A cytopathologist and a hematopathologist reviewed the samples and classified them as “negative,” “atypical,” or “positive.” Whole slide imaging (WSI) was incorporated to quantify atypical lymphocytes using an arbitrary cutoff (≥25% considered positive; <25% considered atypical) and to document necrosis and apoptosis. Ancillary tests included flow cytometry, immunohistochemistry (IHC), and MYD88 mutation analysis.
Results
Of the 226 samples, 214 were diagnosed as negative, 6 as atypical, and 6 as positive. WSI enhanced the diagnosis by precisely quantifying atypical lymphocytes. Flow cytometry was conclusive in 2 of 8 cases, IHC in 7 of 8, and MYD88 analysis in 4 of 5 cases.
Conclusions
While cytology remains the gold standard, a combination of WSI, targeted IHC, and MYD88 analysis enhances diagnostic precision in paucicellular samples. Flow cytometry should be reserved for cases with high cellularity and strong clinical suspicion.
{"title":"Optimal utilization of paucicellular vitreous sample for diagnosis of primary vitreoretinal lymphoma","authors":"Armughan S. Khan MBBS, Anam Khan MD, Zarrin Hossein-Zadeh MD, Lawra Murray CT, MS (ASCP), Karen Chau MBA, CT(ASCP), Atif Khan MD, Xinmin Zhang MD, Rubina S. Cocker MD","doi":"10.1016/j.jasc.2025.05.006","DOIUrl":"10.1016/j.jasc.2025.05.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Primary vitreoretinal lymphoma (PVRL) is a rare, aggressive, and intraocular non-Hodgkin lymphoma, typically manifesting as diffuse large B-cell lymphoma (95%). Vitreous fluid cytology is the gold standard for diagnosis; however, its utility is limited by poor preservation and low cellularity. Recent studies indicate that myeloid differentation primary response protein 88 (MYD88) mutation analysis is more sensitive and accurate on low-cellularity or poorly preserved samples. The incidence of PVRL has reportedly tripled with an annual average of ∼50 cases in the United States. Delayed diagnosis can lead to mortality within 2 years, underscoring the need for improved diagnostic methods.</div></div><div><h3>Materials and methods</h3><div>We conducted a 5-year retrospective study of vitreous samples from 3 tertiary centers. A cytopathologist and a hematopathologist reviewed the samples and classified them as “negative,” “atypical,” or “positive.” Whole slide imaging (WSI) was incorporated to quantify atypical lymphocytes using an arbitrary cutoff (≥25% considered positive; <25% considered atypical) and to document necrosis and apoptosis<span>. Ancillary tests included flow cytometry, immunohistochemistry (IHC), and MYD88 mutation analysis.</span></div></div><div><h3>Results</h3><div>Of the 226 samples, 214 were diagnosed as negative, 6 as atypical, and 6 as positive. WSI enhanced the diagnosis by precisely quantifying atypical lymphocytes. Flow cytometry was conclusive in 2 of 8 cases, IHC in 7 of 8, and MYD88 analysis in 4 of 5 cases.</div></div><div><h3>Conclusions</h3><div>While cytology remains the gold standard, a combination of WSI, targeted IHC, and MYD88 analysis enhances diagnostic precision in paucicellular samples. Flow cytometry should be reserved for cases with high cellularity and strong clinical suspicion.</div></div>","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Pages 317-326"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.06.002
Sinchita Roy-Chowdhuri MD, PhD , Christine N. Booth MD , Jonas J. Heymann MD , Elizabeth Jenkins MS , Joshua R. Menke MD , Sara E. Monaco MD , Ritu Nayar MD , Michiya Nishino MD, PhD , Roberto Ruiz-Cordero MD , Donna K. Russell MEd, CT (ASCP) , Anjali Saqi MD, MBA , Kaitlin E. Sundling MD, PhD , Michael J. Thrall MD , Vanda F. Torous MD , Christopher J. VandenBussche MD, PhD , M. Lisa Zhang MD , Momin T. Siddiqui MD , Paul A. VanderLaan MD, PhD
Introduction
Clinically acquired cytopathology and small biopsy specimens provide essential diagnostic and predictive biomarker information that underlies precision medicine and patient care decisions. Biomarker testing using immunochemistry, in situ hybridization, and molecular analysis is routinely used to inform therapeutic decisions and monitor therapy. Cytopathology and small biopsy specimen collection, handling, and processing vary across different practices and are frequently determined by individual laboratory preference. Thus, clinical specimens are subject to different preanalytical variables that can impact downstream nucleic acid quality and protein antigenicity, compromising the reliability of the ancillary testing results.
Materials and methods
Based on a recent survey by the American Society of Cytopathology there is wide variation in current practices for specimen collection and processing, reflecting a lack of consensus and standardization among cytopathology laboratories. To address this need, the American Society of Cytopathology established a special task force comprising 18 members with expertise and/or interest in ancillary studies in cytopathology and small biopsy specimens.
Results
The task force conducted a survey of existing practices in cytopathology laboratories. A scoping review was performed to identify published literature for relevant evidence focusing on specific areas of interest. The existing literature on preanalytical variables in small specimens and their impact on ancillary studies were reviewed and data were compiled to draft best practice recommendations.
Conclusions
The task force has developed these evidence-based best practice recommendations for optimizing and standardizing preanalytical variables in small specimens to ensure quality and reliability of ancillary studies.
{"title":"Optimizing cytology and small biopsy specimen processing for ancillary studies: recommendations from the American Society of Cytopathology taskforce","authors":"Sinchita Roy-Chowdhuri MD, PhD , Christine N. Booth MD , Jonas J. Heymann MD , Elizabeth Jenkins MS , Joshua R. Menke MD , Sara E. Monaco MD , Ritu Nayar MD , Michiya Nishino MD, PhD , Roberto Ruiz-Cordero MD , Donna K. Russell MEd, CT (ASCP) , Anjali Saqi MD, MBA , Kaitlin E. Sundling MD, PhD , Michael J. Thrall MD , Vanda F. Torous MD , Christopher J. VandenBussche MD, PhD , M. Lisa Zhang MD , Momin T. Siddiqui MD , Paul A. VanderLaan MD, PhD","doi":"10.1016/j.jasc.2025.06.002","DOIUrl":"10.1016/j.jasc.2025.06.002","url":null,"abstract":"<div><h3>Introduction</h3><div>Clinically acquired cytopathology and small biopsy specimens provide essential diagnostic and predictive biomarker information that underlies precision medicine and patient care decisions. Biomarker testing using immunochemistry, in situ hybridization, and molecular analysis is routinely used to inform therapeutic decisions and monitor therapy. Cytopathology and small biopsy specimen collection, handling, and processing vary across different practices and are frequently determined by individual laboratory preference. Thus, clinical specimens are subject to different preanalytical variables that can impact downstream nucleic acid quality and protein antigenicity, compromising the reliability of the ancillary testing results.</div></div><div><h3>Materials and methods</h3><div>Based on a recent survey by the American Society of Cytopathology there is wide variation in current practices for specimen collection and processing, reflecting a lack of consensus and standardization among cytopathology laboratories. To address this need, the American Society of Cytopathology established a special task force comprising 18 members with expertise and/or interest in ancillary studies in cytopathology and small biopsy specimens.</div></div><div><h3>Results</h3><div>The task force conducted a survey of existing practices in cytopathology laboratories. A scoping review was performed to identify published literature for relevant evidence focusing on specific areas of interest. The existing literature on preanalytical variables in small specimens and their impact on ancillary studies were reviewed and data were compiled to draft best practice recommendations.</div></div><div><h3>Conclusions</h3><div>The task force has developed these evidence-based best practice recommendations for optimizing and standardizing preanalytical variables in small specimens to ensure quality and reliability of ancillary studies.</div></div>","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Pages 285-308"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anal Cytology and High-Risk HPV Cotesting: Clinical Features and Histologic Correlation","authors":"Bilge Dundar MD, Yi Zhu MD, PhD, Chuan Chen MD, PhD, Meredith VandeHaar SCT(ASCP), Kristina Lewis CT(ASCP), Erin Ulrich SCT(ASCP), Amy Swanson MD, Diva Salomao MD, Yajue Huang MD, PhD","doi":"10.1016/j.jasc.2025.07.073","DOIUrl":"10.1016/j.jasc.2025.07.073","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Page S44"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145099954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.07.045
Shilpa Reddy DO, Kamal Khurana MD, Cindy A. Steele SCT(ASCP), Patrick Fasulo BS, CT(ASCP)
{"title":"Diagnostic Utility of Touch Imprint Cytology of Core Needle Biopsy of Lung Masses: A Comparative Analysis of CT-Guided Versus Endobronchial Ultrasound-Guided Lung Core Needle Biopsies","authors":"Shilpa Reddy DO, Kamal Khurana MD, Cindy A. Steele SCT(ASCP), Patrick Fasulo BS, CT(ASCP)","doi":"10.1016/j.jasc.2025.07.045","DOIUrl":"10.1016/j.jasc.2025.07.045","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Page S30"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.07.040
Niraj Vyas MD, MBS, Juan Xing MD
{"title":"Seven-Year Institutional Experience in Subtyping of Salivary Gland Neoplasm of Uncertain Malignant Potential","authors":"Niraj Vyas MD, MBS, Juan Xing MD","doi":"10.1016/j.jasc.2025.07.040","DOIUrl":"10.1016/j.jasc.2025.07.040","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Pages S27-S28"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.07.026
Mohammed Ahmed MD, Dianna Almaraz-Klippel MS, Gloria Sura MD, Uma Kundu MD, Wendong Yu MD, PhD, John Stewart MD, PhD, Qiong Gan MD, Savitri Krishnamurthy MD
{"title":"Prospective Multi-Reader Study for Evaluating the Feasibility of Whole Slide Imaging for Rapid Onsite Evaluation of Thyroid Fine Needle Aspiration","authors":"Mohammed Ahmed MD, Dianna Almaraz-Klippel MS, Gloria Sura MD, Uma Kundu MD, Wendong Yu MD, PhD, John Stewart MD, PhD, Qiong Gan MD, Savitri Krishnamurthy MD","doi":"10.1016/j.jasc.2025.07.026","DOIUrl":"10.1016/j.jasc.2025.07.026","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Pages S20-S21"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.07.068
Sean Byrne DO, Jason Willis CT(ASCP), MBA, Mohamad Gafeer MD, Swati Satturwar MD, Hamza Gokozan MD
{"title":"Temporal Trends of Abnormal Cervical Cytology in an Underserved Free Clinic Population: A Six-Year Experience","authors":"Sean Byrne DO, Jason Willis CT(ASCP), MBA, Mohamad Gafeer MD, Swati Satturwar MD, Hamza Gokozan MD","doi":"10.1016/j.jasc.2025.07.068","DOIUrl":"10.1016/j.jasc.2025.07.068","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Page S42"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"WHO Reporting System for Pancreaticobiliary Cytopathology: Correlation with Malignancy and Survival","authors":"Nadwa Bustami MD, Christopher Moroz MD, Vijayalakshmi Padmanabhan MBBS, MD, MPH","doi":"10.1016/j.jasc.2025.07.017","DOIUrl":"10.1016/j.jasc.2025.07.017","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Pages S15-S16"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.07.018
Kelsey McHugh MD , Maria Luisa Policarpio-Nicolas MD
{"title":"Cytologic, Clinicopathologic and Radiologic Findings of Pancreatic Solitary Fibrous Tumor: Our Institutional Experience","authors":"Kelsey McHugh MD , Maria Luisa Policarpio-Nicolas MD","doi":"10.1016/j.jasc.2025.07.018","DOIUrl":"10.1016/j.jasc.2025.07.018","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Page S16"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.1016/j.jasc.2025.07.061
Patrick Bladek MD, Kristen Bevans-Wilkins DHSc, MBA, SCT(ASCP), Julian Tan DO, Robert Cabay MD, DDS, Odile David MD
{"title":"Follow-Up Outcomes of High Grade Squamous Intraepithelial Lesion (HSIL) Cytology with High-Risk Human Papillomavirus (HR HPV) Genotypes Other Than 16/18","authors":"Patrick Bladek MD, Kristen Bevans-Wilkins DHSc, MBA, SCT(ASCP), Julian Tan DO, Robert Cabay MD, DDS, Odile David MD","doi":"10.1016/j.jasc.2025.07.061","DOIUrl":"10.1016/j.jasc.2025.07.061","url":null,"abstract":"","PeriodicalId":38262,"journal":{"name":"Journal of the American Society of Cytopathology","volume":"14 5","pages":"Page S39"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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