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Preparation and application of oil-in-water structure linseed oil- EGCG nanoemulsion 水包油结构亚麻籽油-EGCG 纳米乳液的制备与应用
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116318
Dongchao Xie , Yuan Wang , Yingying Zhou, Yan Zhu, Yahui Zhang, Haihua Zhang, Qizhen Du, Peng Jin

The oil–in–water nanoemulsion of linseed oil and EGCG (LOE-NE) was successfully constructed by ultrasonic treatment. Optimized formula successfully prepared LOE–NE with excellent physicochemical characterization, average particle diameter 160–180 nm, PDI <0.2, Zeta potential ≈−60. Results showed that oil–water ratio had significant effect on the stability of LOE-NE but EGCG did not. Comprehensive characterization and nutritional contribution showed that the emulsion with 1:9 oil–water ratio and 1% EGCG was the best formula. The preferred LOE-NE at low temperature were conducive to reducing the EGCG loss and inhibiting its browning during long-term storage. Further application of the LOE-NE to high–oil foods indicated that addition of 10% LOE-NE and 0.1% neohesperidin dihydrochalcone (NHDC) to salad sauce could improve the sensory flavor, significantly enhancing the antibacterial and antioxidant activities and prolonging its quality stability. Moreover, the addition of LOE-NE can slow down the accumulation of acrylamide and reduce the loss of representative unsaturated fatty acids in the high–temperature baking of mooncakes. These findings will provide new methods and ideas for expanding the food application of high-nutrition linseed oil, and can offer theoretical support and application guidance for the practical development and utilization of nanoemulsion.

通过超声波处理,成功构建了亚麻籽油和EGCG的水包油纳米乳液(LOE-NE)。优化配方成功制备的 LOE-NE 具有优异的理化特性,平均粒径为 160-180 nm,PDI 为 0.2,Zeta 电位≈-60。结果表明,油水比对 LOE-NE 的稳定性有显著影响,但 EGCG 没有影响。综合表征和营养成分显示,油水比为 1:9 和 EGCG 含量为 1%的乳液是最佳配方。低温条件下的 LOE-NE 更有利于减少 EGCG 的损失,并抑制其在长期储存过程中的褐变。LOE-NE 在高油食品中的进一步应用表明,在沙拉酱中添加 10% 的 LOE-NE 和 0.1% 的新橙皮甙二氢查尔酮(NHDC)可改善感官风味,显著提高抗菌和抗氧化活性,延长其质量稳定性。此外,在月饼的高温烘焙过程中,添加 LOE-NE 可以减缓丙烯酰胺的积累,减少代表性不饱和脂肪酸的损失。这些研究结果将为拓展高营养亚麻籽油的食品应用提供新的方法和思路,为纳米乳液的实际开发和利用提供理论支持和应用指导。
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引用次数: 0
Are the sensory qualities of prestigious traditional varieties of beans preserved in commercial canned products? A comprehensive sensorial and chemical study on Ganxet beans 商业罐头产品是否保留了著名传统豆类品种的感官品质?对甘蔗豆的感官和化学综合研究
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116413
Salvador Roselló , Joaquin Beltran , Ana Rivera , Laura López-Mas , Roser Romero del Castillo , Joan Casals

The commercial production of legume cans often involves exposing products to elevated temperatures, albeit with potential alterations to sensory traits. For premium products such as the Ganxet bean, which holds a Protected Designation of Origin (PDO) quality label, these adverse effects may influence consumer acceptance. This study investigated sensory and chemical differences between commercial cans and traditionally cooked Ganxet beans. A market survey enabled the identification of 15 commercial Ganxet cans, which were profiled for different physicochemical traits. Five were selected to represent maximum variability and were compared to two traditionally cooked references using standardized descriptive sensory and compositional analyses (volatile and non-volatile fractions). Commercial cans presented significantly higher off-flavor intensity and product darkening, while no significant changes were observed for textural traits. Commercial cans exhibited a broad diversity in their volatile profile and significantly lower content of distinctive non-volatile molecules (citric acid, sucrose, raffinose, and stachyose). A multivariate PLS regression model related off-flavor present in commercial cans with (E,E)-2,4-heptadienal, (E)-2-octenal, 1-nonanol (enhancers), and glutamic, malic, (E,E)-2,4-nonadienal, stachyose, sucrose, and glucose (inhibitors). The deviation of commercial Ganxet cans from PDO sensory quality requirements highlights the necessity for innovations in cooking protocols. These innovations are essential for producing high-quality cans from prestigious bean varieties.

豆类罐头的商业化生产通常需要将产品暴露在高温下,尽管这可能会改变产品的感官特征。对于像甘蔗豆这样拥有原产地名称保护(PDO)质量标签的优质产品来说,这些不利影响可能会影响消费者的接受程度。本研究调查了商业罐装甘蔗豆和传统烹饪甘蔗豆之间的感官和化学差异。通过市场调查,确定了 15 种商业甘赛特罐头,并对其进行了不同理化特性的分析。通过标准化的描述性感官和成分分析(挥发性和非挥发性部分),将其中五种罐头与两种传统烹饪的参考罐头进行了比较。商用罐头的异味强度和产品变暗程度明显较高,而在质地特征方面没有观察到明显变化。商用罐头的挥发性特征具有广泛的多样性,而独特的非挥发性分子(柠檬酸、蔗糖、棉子糖和水苏糖)的含量则明显较低。一个多变量 PLS 回归模型将商品罐中的异味与(E,E)-2,4-庚二烯醛、(E)-2-辛烯醛、1-壬醇(增强剂)以及谷氨酸、苹果酸、(E,E)-2,4-壬二烯醛、水苏糖、蔗糖和葡萄糖(抑制剂)联系起来。商用甘赛特罐头与 PDO 感官质量要求的偏差突出表明了对烹饪规程进行创新的必要性。这些创新对于利用著名豆类品种生产优质罐头至关重要。
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引用次数: 0
Antioxidant potential of pomegranate peel and acorn cupule extracts: A comparative analysis in simulated In vitro digestion of uncured dry sausages 石榴皮和橡子壳提取物的抗氧化潜力:模拟未腌制干香肠体外消化的比较分析
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116371
Guadalupe Lavado, Ramón Cava

The study investigated the effects of incorporating quercetin and varying amounts of polyphenols (0, 1.25, and 12.5 mg/digestion) from two natural sources, pomegranate peel (PPE) and acorn cupule (ACE), on lipid and protein oxidation during gastrointestinal digestion of uncured dry sausages. Quercetin significantly reduced levels of lipid and protein oxidation markers, as well as the levels of PPE and ACE added, compared to the control. The antioxidant effects of PPE and ACE were dose-dependent. The greater the amount of added polyphenols (12.5 vs. 1.25 mg/digest), the lower the oxidation. ACE exhibited a greater antioxidant effect than PPE, reducing the formation of malondialdehyde in the digests. Conversely, PPE demonstrated a stronger antioxidant effect than ACE in terms of protein oxidation, decreasing the formation of carbonyl groups in the digests. Our study underscores the critical need to address the potential health risks associated with consuming uncured dry sausages, emphasizing the remarkable antioxidant capabilities of pomegranate peel extract and acorn cupule extract, which offer significant protective effects against lipid and protein oxidation during digestion, highlighting the importance of promoting consumer awareness regarding antioxidant-rich dietary choices to mitigate harmful compound formation.

该研究调查了在未腌制干香肠的胃肠道消化过程中,添加槲皮素和不同量的多酚(0、1.25 和 12.5 毫克/消化)对脂质和蛋白质氧化的影响,这两种多酚来自石榴皮(PPE)和橡子壳(ACE)。与对照组相比,槲皮素能明显降低脂质和蛋白质氧化标记物的水平,并降低石榴皮和橡实杯突的添加水平。PPE 和 ACE 的抗氧化作用与剂量有关。添加的多酚含量越高(12.5 与 1.25 毫克/消化物),氧化作用就越低。与 PPE 相比,ACE 的抗氧化效果更强,可减少消化物中丙二醛的形成。相反,就蛋白质氧化而言,PPE 比 ACE 表现出更强的抗氧化效果,可减少消化物中羰基的形成。我们的研究强调了解决与食用未腌制干香肠相关的潜在健康风险的迫切需要,强调了石榴皮提取物和橡子壳提取物的显著抗氧化能力,它们对消化过程中的脂质和蛋白质氧化具有显著的保护作用,强调了提高消费者对富含抗氧化剂的膳食选择的认识以减少有害化合物形成的重要性。
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引用次数: 0
A combination of high-voltage atmospheric cold plasma and cinnamaldehyde significantly increases inactivation of Salmonella enterica and Escherichia coli O157:H7 in raw pineapple juice 高压常压冷等离子体和肉桂醛的组合可显著提高生菠萝汁中肠炎沙门氏菌和大肠杆菌 O157:H7 的灭活能力
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116380
Braden Lewis , Aubrey Mendonca , Paulo Fortes-Da-Silva , Terri Boylston , Allison Little , Byron Brehm-Stecher , Shannon Coleman , Armitra Jackson-Davis

The main objective of this study was to determine the antibacterial effectiveness of high voltage atmospheric cold plasma (HVACP) alone or combined with cinnamaldehyde against S. enterica and E. coli O157:H7 in raw pineapple juice. Additional objectives were to evaluate sublethal injury in pathogen survivors and physicochemical properties of the juice. Juice with or without added cinnamaldehyde (100 μL/L) and inoculated with ∼7.0 log10 CFU/mL of S. enterica or E. coli O157:H7, was exposed to HVACP (70 kV, 60 Hz) for 0 (control), 1.0, 3.0, 5.0, and 7.0 min. HVACP or cinnamaldehyde alone failed to achieve a 5.0-log10 CFU/mL reduction of the pathogens. In contrast, reductions (log10 CFU/mL) from combined application of HVACP and cinnamaldehyde were 5.38 (S. enterica) and 5.60 (E. coli O157:H7) after 7.0 min (P < 0.05) with significant sublethal injury in S. enterica survivors (P < 0.05). The degrees Brix increased only in HVACP-treated juice without cinnamaldehyde (P < 0.05). HVACP treatment for 3 or 7 min, decreased lightness but increased chroma of the juice (P < 0.05); however, color differences were not visually detectable. The combined use of HVACP and cinnamaldehyde can enhance the microbial safety of pineapple juice with minimal changes in the physiochemical properties evaluated in this study.

本研究的主要目的是确定高压大气冷等离子体 (HVACP) 单独或与肉桂醛结合使用对生菠萝汁中肠道病毒和大肠杆菌 O157:H7 的抗菌效果。其他目标还包括评估病原体幸存者的亚致死损伤以及果汁的理化特性。将添加或不添加肉桂醛(100 μL/L)并接种了 ∼7.0 log10 CFU/mL 肠杆菌或大肠杆菌 O157:H7 的果汁暴露于 HVACP(70 kV,60 Hz),时间分别为 0(对照组)、1.0、3.0、5.0 和 7.0 分钟。单独使用 HVACP 或肉桂醛都无法使病原体减少 5.0-log10 CFU/mL。相反,在 7.0 分钟后,HVACP 和肉桂醛联合应用的减少量(log10 CFU/mL)分别为 5.38(肠球菌)和 5.60(大肠杆菌 O157:H7)(P < 0.05),肠球菌幸存者受到了显著的亚致死伤害(P < 0.05)。只有在不含肉桂醛的 HVACP 处理果汁中,Brix 度才会增加(P < 0.05)。HVACP 处理 3 或 7 分钟会降低果汁的亮度,但会增加色度(P < 0.05);不过,肉眼无法察觉颜色差异。联合使用 HVACP 和肉桂醛可提高菠萝汁的微生物安全性,而对本研究中评估的理化特性的改变却很小。
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引用次数: 0
Machine learning for authentication of black tea from narrow-geographic origins: Combination of PCA and PLS with LDA and SVM classifiers 机器学习用于鉴定产地狭窄的红茶:将 PCA 和 PLS 与 LDA 和 SVM 分类器相结合
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116401
Nahid Mohammadi , Mahnaz Esteki , Jesus Simal-Gandara

This study investigates the feasibility of using UV–Vis spectroscopy coupled with machine learning methods to authenticate tea samples based on their geographical origins in a narrow longitudinal strip (200 km). Several preprocessing methods, such as standard normal variate (SNV), auto-scaling, multiplicative scatter correction (MSC), mean centring (MC), first derivative, and their combinations, were applied to eliminate the noninformative information. The partial least squares-linear discriminant analysis (PLS-LDA) model using first derivative spectra represented the following results, including 98.0% sensitivity, 99.5% specificity, and a mean accuracy of 98.0%. The support vector machine (PLS-SVM) classifier using first derivative spectra represented 94.0% sensitivity, 98.6% specificity, and a mean accuracy of 94.0%. The satisfactory results of the models depicted that the chemical components of tea, such as polyphenols, chlorogenic and fatty acids that absorb UV radiation are the chemical markers that can discriminate tea samples based on their geographical origin. Therefore, UV–Vis spectral fingerprinting combined with machine learning methods could be a practical, feasible, and simple method for classifying tea based on their geographical origins in a narrow longitudinal strip.

本研究探讨了利用紫外可见光谱与机器学习方法相结合,根据狭长纵带(200 千米)上茶叶的地理来源鉴定茶叶样品的可行性。应用了几种预处理方法,如标准正态变异(SNV)、自动缩放、乘法散度校正(MSC)、均值中心化(MC)、一阶导数及其组合,以消除非信息信息。使用一阶导数光谱的偏最小二乘法线性判别分析(PLS-LDA)模型代表了以下结果,包括 98.0% 的灵敏度、99.5% 的特异性和 98.0% 的平均准确度。使用一阶导数光谱的支持向量机(PLS-SVM)分类器的灵敏度为 94.0%,特异度为 98.6%,平均准确率为 94.0%。这些模型令人满意的结果表明,茶叶中吸收紫外线辐射的化学成分,如茶多酚、绿原酸和脂肪酸,是可以根据茶叶的地理来源区分茶叶样品的化学标记。因此,紫外可见光谱指纹图谱与机器学习方法相结合,可以成为一种实用、可行且简单的方法,用于根据窄纵带茶叶的地理产地进行分类。
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引用次数: 0
Evaluation of different roasting condition on yield, physico-chemical characteristics, and antioxidant activity of cold-pressed sacha inchi (Plukenetia volubilis) oil 评估不同焙烧条件对冷压沙棘油的产量、理化特性和抗氧化活性的影响
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116343
Yung-Jia Chan , Chien-Shan Chiu , Po-Hsien Li , Wen-Chien Lu

Sacha inchi (Plukenetia volubilis) seed oil, which is extracted from sacha inchi seeds, may possibly establish a nutritive source which prosperous of nutraceutical value. It is paramount to assess the oil production potential of industrial oilseeds for manufacturing applications. Roasting is a key procedure for preparing oils of plant origin, which leads to crucial changes in the oil quality. With the aim of evaluating new sources for the industrial production of edible oil, the effect of different temperatures (60, 80, 100, and 120 °C) and times (10, 20, and 30 min) of roasting as an important pretreatment was evaluated on the yield, physical-chemical and qualitative properties of cold-pressed sacha inchi seed oil. Results showed that the oil yield, acid value (AV), peroxide value (PV), p-anisidine value, and oil stability index (OSI) increased, whereas the iodine value (IV) and conjugated diene (CD) content decreased with treatment temperature and time. There were no obvious different in the fatty acid composition. Prolonged roasting temperature and time increased tocopherol, phytosterol, squalene, total phenolic content (TPC), and antioxidant activity. Current findings indicated that thermal processing may have positive effect towards the quality and nutritional content of sacha inchi seed oil.

从沙棘籽中提取的沙棘籽油可能是一种营养来源,具有丰富的营养保健价值。评估工业油籽的产油潜力对于生产应用至关重要。焙烧是制备植物油的关键步骤,会导致油质发生重要变化。为了评估工业化生产食用油的新来源,研究人员评估了不同温度(60、80、100 和 120 °C)和不同时间(10、20 和 30 分钟)的焙烧作为重要预处理对冷榨山茶籽油的产量、物理化学和质量特性的影响。结果表明,随着处理温度和时间的升高,出油率、酸值(AV)、过氧化值(PV)、对甲氧基苯胺值和油稳定性指数(OSI)都有所提高,而碘值(IV)和共轭二烯(CD)含量则有所下降。脂肪酸组成没有明显差异。延长烘烤温度和时间可增加生育酚、植物甾醇、角鲨烯、总酚含量(TPC)和抗氧化活性。目前的研究结果表明,热加工可能会对沙棘籽油的质量和营养成分产生积极影响。
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引用次数: 0
Physicochemical characteristics and microbial diversity of sous vide scallops (Chlamys farreri) during chilled storage 冷藏期间带子(Chlamys farreri)的理化特性和微生物多样性
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-01 DOI: 10.1016/j.lwt.2024.116437
Meiqi Gu, Chuanhai Tu, Huili Jiang, Ting Li, Ning Xu, Shanshan Shui, Soottawat Benjakul, Bin Zhang
This study investigated the impact of sous vide (SV) cooking methods on the physical and chemical characteristics as well as the microbial composition of scallops () over a 30 days period of refrigerated storage. Scallop treatments were divided into two groups: CS (scallops vacuum-packed and then steamed for 30 min at 75 °C) and CK (scallops steamed for 15 min at 100 °C). With the increase in refrigeration time, the total volatile basic nitrogen (TVBN), pH, cooking loss, microbial content, and malondialdehyde (MDA) content gradually increased, while the water-soluble protein extraction rate and the myofibrillar protein (MP) extraction rate of the CS and CK samples significantly decreased. Notably, the SV steaming treatment effectively reduced microbial degradation of proteins, improved water retention of scallop samples, and maintained high MP extraction as compared to CK samples (MP content ranged from 1.09% to 2.50% in the CK group and 1.75%–3.33% in the CS group). During the late stages of refrigeration, conventionally cooked scallops (CK) were dominated by and , with the ability to produce biogenic amines, while SV steamed scallops were dominated by . Furthermore, a link was discovered between the microbial community and the physicochemical parameters.
本研究调查了在 30 天的冷藏贮存期内,苏式蒸煮(SV)方法对扇贝()的物理和化学特性以及微生物组成的影响。扇贝处理分为两组:CS 组(扇贝真空包装,然后在 75 °C 下蒸煮 30 分钟)和 CK 组(扇贝在 100 °C 下蒸煮 15 分钟)。随着冷藏时间的延长,CS 和 CK 样品的总挥发性碱基氮(TVBN)、pH 值、蒸煮损失、微生物含量和丙二醛(MDA)含量逐渐增加,而水溶性蛋白提取率和肌纤维蛋白(MP)提取率则明显下降。值得注意的是,与 CK 样品相比,SV 蒸煮处理有效地减少了蛋白质的微生物降解,提高了扇贝样品的保水性,并保持了较高的 MP 提取率(CK 组的 MP 含量为 1.09% 至 2.50%,CS 组为 1.75% 至 3.33%)。在冷藏后期,传统烹饪的扇贝(CK)中主要是Ⅴ和Ⅴ,具有产生生物胺的能力,而 SV 蒸扇贝中主要是Ⅴ和Ⅴ。此外,还发现了微生物群落与理化参数之间的联系。
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引用次数: 0
Effects of Eucommia ulmoides leaf powder on the gelatinization, rheology, and gel properties of sweet potato starch 杜仲叶粉对甘薯淀粉糊化、流变学和凝胶特性的影响
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-30 DOI: 10.1016/j.lwt.2024.116428
Sihai Han, Yueyun Wu, Shuming Chen, Jian Wang, Zhouya Bai, Peiyan Li, Chonghui Yue, Denglin Luo
This study aimed to explore the feasibility of employing Eucommia ulmoides leaf powder (ELP), which is a novel medicinal and food homologous material, to improve the physicochemical properties of sweet potato starch (SPS) gel. The effects of ELP on the gelatinization, rheology, and gel properties of SPS were investigated. The Brabander viscosity test results showed that ELP could reduce the peak viscosity, trough viscosity, final viscosity, and setback value of SPS. The rheological tests showed that the SPS-ELP gels had shear-thinning behavior, and that the ELP could decrease the apparent viscosity, shear stress, G′, G″ of SPS-ELP gels and make the gels exhibit stronger liquid-like behavior. The X-ray diffraction pattern indicated that the ELP could decrease the relative crystallinity and retard retrogradation of SPS-ELP gels, which was cofirmed by microscopic structure analysis. The ELP had a significant effect on the color difference of the gels, resulting in a yellow-green color. The addition of ELP also reduced the hardness of SPS-ELP gels, and increased the median particle size D50, volume mean diameter D [4,3], and area mean diameter D [3,2] of gel particle size distribution. These findings suggest that ELP has a good potential in improving starch gel properties.
本研究旨在探讨杜仲叶粉(ELP)这一新型药食同源材料用于改善甘薯淀粉(SPS)凝胶理化性质的可行性。研究了 ELP 对 SPS 的糊化、流变和凝胶特性的影响。Brabander 粘度测试结果表明,ELP 能降低 SPS 的峰值粘度、谷值粘度、最终粘度和后退值。流变测试表明,SPS-ELP凝胶具有剪切稀化行为,ELP能降低SPS-ELP凝胶的表观粘度、剪切应力、G′、G″,并使凝胶表现出更强的液态行为。X 射线衍射图样表明,ELP 能降低 SPS-ELP 凝胶的相对结晶度并延缓其逆变,这一点在显微结构分析中得到了证实。ELP 对凝胶的色差有明显影响,使凝胶呈现黄绿色。添加 ELP 还降低了 SPS-ELP 凝胶的硬度,增加了凝胶粒度分布的中值粒径 D50、体积平均直径 D [4,3]和面积平均直径 D [3,2]。这些发现表明,ELP 在改善淀粉凝胶性能方面具有良好的潜力。
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引用次数: 0
Development and verification of crossing priming amplification on rapid detection of virulent and viable L. monocytogenes: In-depth analysis on the target and further application on food screening 交叉引物扩增技术的开发与验证,用于快速检测单核细胞增生症病毒和存活的单核细胞增生症:目标深入分析及在食品筛查中的进一步应用
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-30 DOI: 10.1016/j.lwt.2024.116422
Junyan Liu, Zhufang Xiang, Tengyi Huang, Zhenbo Xu, Qin Ma, Lei Yuan, Thanapop Soteyome
Virulent is responsible for listeriosis, a highly fatal foodborne illness that impairs human health. One of the major causes of its pathogenicity is the carriage of virulence factor, listeriolysin O, encoded by gene. Herein, the specificity and conservativity of gene sequences were analyzed, with most conservative region selected. Then, a crossing priming amplification (CPA) assay targeting conservative region was established under an isothermal condition (63 °C) to identify virulent within 60 min. Forty-four reference strains including two standard strains and forty-two strains of other species were selected to assess sensitivity and specificity. The CPA assay showed strong specificity and sensitivity (genomic DNA at 5.66 pg/μL). The practicality of the CPA assay was further confirmed in four artificially contaminated rice-flour products with limit of detection at 10 CFU/mL. In addition, a PMA-CPA assay was developed to direct detect viable cells with rice-flour products as a model system. In conclusion, the developed CPA method has advantages of high sensitivity, specificity and quick measurement in the detection of virulent , showing considerably feasible promise for future food safety.
李斯特菌病是一种高度致命的食源性疾病,损害人类健康。其致病性的主要原因之一是携带由基因编码的毒力因子李斯特溶菌素 O。本文分析了基因序列的特异性和保守性,选择了最保守的区域。然后,在等温条件(63 °C)下建立了针对保守区的交叉引物扩增(CPA)检测方法,以在 60 分钟内鉴定毒力。为了评估灵敏度和特异性,研究人员选择了 44 株参考菌株(包括 2 株标准菌株和 42 株其他物种菌株)。CPA 检测法显示出很强的特异性和灵敏度(基因组 DNA 为 5.66 pg/μL)。在四种人为污染的米粉产品中进一步证实了 CPA 检测法的实用性,其检测限为 10 CFU/mL。此外,还开发了一种 PMA-CPA 检测法,以米粉产品为模型系统直接检测有活力的细胞。总之,所开发的 CPA 方法具有灵敏度高、特异性强、测量快速等优点,可用于检测毒性大肠杆菌、大肠杆菌毒素和大肠杆菌病毒,在未来的食品安全中具有相当大的可行性。
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引用次数: 0
Generation of wine sensory descriptors by one-step method based on free profile and their use to establish the typicity of PDO wines 根据自由曲线一步法生成葡萄酒感官描述符,并将其用于确定 PDO 葡萄酒的类型
IF 6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-27 DOI: 10.1016/j.lwt.2024.116410
Miriam Ortega-Heras, María Curiel-Fernández, Silvia Pérez-Magariño, María Luisa González-Sanjosé
The typicity of wines is related to their sensory characteristics, which allow them to be differentiated from other wines. Different sensory methods have been applied to try to detect the sensory characteristics responsible for the typicity of Protected Designation of Origin wines (PDO). However, so far, none of them has been completely successful. In this study, a new one-step method based on free profile and comparative analysis by pairs was applied to generate sensory descriptors of wines. The discriminant power of the sensory attributes generated among wines from 4 different PDO wines from the Spanish Autonomous Community of Castilla y León was evaluated. The study was carried out with wine experts and 159 white, rosé, and red wines were tasted. The judges generated 245 attributes which were reduced to 95 (11 defects, 20 visual, 48 olfactory and 16 taste and mouthfeel). A Multifactorial Analysis was carried out with the data of contingency matrices based on the frequency of citation. The results obtained showed that the sensory descriptors generated allowed the wines to be distinguished according to their origin, being the discriminant power significantly higher when the visual, olfactory and gustatory attributes were considered separately.
葡萄酒的典型性与其感官特征有关,感官特征使其能够与其他葡萄酒区分开来。为了检测原产地名称保护葡萄酒(PDO)典型性的感官特征,人们采用了不同的感官方法。然而,迄今为止,没有一种方法是完全成功的。在这项研究中,采用了一种基于自由剖面和成对比较分析的新的一步法来生成葡萄酒的感官描述符。对来自西班牙卡斯蒂利亚-莱昂自治区 4 种不同法定产区的葡萄酒所产生的感官属性的判别能力进行了评估。这项研究由葡萄酒专家进行,共品尝了 159 种白葡萄酒、桃红葡萄酒和红葡萄酒。评委们提出了 245 项属性,最后减少到 95 项(11 项缺陷、20 项视觉、48 项嗅觉、16 项味觉和口感)。对基于引用频率的或然矩阵数据进行了多因素分析。结果表明,所生成的感官描述符可以根据葡萄酒的产地对其进行区分,当分别考虑视觉、嗅觉和味觉属性时,区分度明显更高。
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LWT - Food Science and Technology
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