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Postbiotics for improving food quality and safety: From mechanistic insights to industrial applications 提高食品质量和安全的后生物制剂:从机械洞察到工业应用
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.lwt.2026.119066
Sarika Vishnu Pawar, Shlomo Sela Saldinger, Moshe Shemesh
Growing consumer demand for nutritious, safe, and sustainable foods is accelerating the shift toward green preservation technologies and bio-based additives. Among natural microbial derivatives, postbiotics—the non-viable microbial cells, metabolites, and cell components released after cell lysis—are emerging as stable and safe bioprotective agents. Unlike live probiotics or prebiotics, postbiotics are environmentally resilient, free from infectivity risks, and retain their functional bioactivity under industrial processing conditions. Their antimicrobial, antioxidant, and immunomodulatory properties support their broad application in food safety enhancement and biopreservation. This review brings together current insights into the composition, bioactivity, and functionality of postbiotics across diverse food systems, emphasizing recent advances in their characterization and quantification and our understanding of the mechanisms by which they act. It also critically evaluates major challenges related to strain specificity, substrate influences, analytical standardization, regulatory alignment, and mechanistic insights regarding postbiotic functionality, underscoring key scientific research gaps that must be addressed to enable their full-scale industrial adoption. Despite these limitations, postbiotics offer a promising, sustainable alternative for food safety and preservation and warrant further in-depth research and development to enable their successful commercialization.
消费者对营养、安全和可持续食品日益增长的需求正在加速向绿色保鲜技术和生物基添加剂的转变。在天然微生物衍生物中,后生物制剂——非活的微生物细胞、代谢物和细胞裂解后释放的细胞成分——正在成为稳定和安全的生物保护剂。与活的益生菌或益生元不同,后益生菌具有环境弹性,没有感染风险,并在工业加工条件下保持其功能性生物活性。其抗菌、抗氧化和免疫调节特性支持其在食品安全和生物保存方面的广泛应用。这篇综述汇集了目前对不同食物系统中后生物制剂的组成、生物活性和功能的见解,强调了它们的表征和量化的最新进展以及我们对它们作用机制的理解。它还批判性地评估了与菌株特异性、底物影响、分析标准化、监管一致性和关于后生物功能的机制见解相关的主要挑战,强调了必须解决的关键科学研究差距,以使其能够全面的工业应用。尽管存在这些限制,后生物制剂为食品安全和保存提供了一个有前途的、可持续的替代方案,值得进一步深入研究和开发,以使其成功商业化。
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引用次数: 0
Quality of farmed Atlantic halibut (Hippoglossus hippoglossus); a comparative study of sub-chilling in refrigerated saltwater vs traditional ice chilling 养殖大西洋大比目鱼的质量冷冻盐水亚冷与传统冰冷的比较研究
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119057
Trond Løvdal , Frida Bårdsen , Bjørn Tore Rotabakk , Atle Foss , Børre Erstad , Bjorn Roth
Atlantic halibut (Hippoglossus hippoglossus) were slaughtered and sub-chilled in refrigerated seawater (˗1.5 °C) for 24 h prior to packaging in expanded polystyrene (EPS) boxes with or without ice. These were compared to halibut traditionally packaged directly in EPS boxes with ice, without prior sub-chilling. All boxes were stored at 4 °C for 24 h and thereafter at 0 °C. The microbial growth in halibut flesh was analysed by traditional plate count methods and skin samples were analysed for total bacteria and selected spoilage bacteria by quantitative Polymerase Chain Reaction. Quality Index Method (QIM) was performed on whole halibut as a proxy for sensorial quality. After 21 days of storage, total bacteria count in traditionally iced halibut was log 7.07 colony forming units (cfu) per gram, significantly higher than both sub-chilled groups; 5.60 (without ice) and 5.56 cfu/g (with ice). Hence, microbial shelf-life for both sub-chilled groups were estimated to 23 days, compared to 17 days for traditionally stored halibut, based on linear regression for reaching log 6 bacteria per gram. QIM-analysis after 22 days of storage revealed that sub-chilled halibut had significantly lower QIM-score than traditionally iced halibut. This included smell, physical appearance (eyes and skin), and mucus.
将大西洋大比目鱼(Hippoglossus Hippoglossus)屠宰后,置于冷冻海水(2.7°C)中冷冻24小时,然后用发泡聚苯乙烯(EPS)盒装入或不装冰。将这些比目鱼与传统上直接包装在带有冰的EPS盒中的比目鱼进行比较,而无需事先进行亚冷却。所有盒子在4°C下保存24 h,然后在0°C下保存。采用传统的平板计数法对大比目鱼肉中的微生物生长情况进行了分析,并用定量聚合酶链式反应对皮肤样品中的总细菌和选定的腐败细菌进行了分析。采用质量指数法(QIM)对整个比目鱼进行感官质量评价。贮藏21 d后,传统冷藏大比目鱼菌落形成单位(cfu)为每克7.07个,显著高于亚冷藏组;5.60(不加冰)和5.56 cfu/g(加冰)。因此,根据线性回归,两个亚冷藏组的微生物保质期估计为23天,而传统储存的大比目鱼为17天,达到每克log 6个细菌。贮藏22天后的qim分析显示,亚冷藏比目鱼的qim评分明显低于传统冷藏比目鱼。这包括气味、外表(眼睛和皮肤)和粘液。
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引用次数: 0
Effect of air frying instead of deep frying on the quality and crust characteristics of French fries 空气煎炸代替油炸对薯条品质及脆皮特性的影响
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119059
Yunyun Zheng , Rana Muhammad Aadil , Guanyu Fang , Qingjian Shen , Zhiping He , Xingquan Liu , Cheng Zhang
The widespread consumption of French fries across diverse global populations is tempered by notable health concerns associated with their high oil content. This study investigated the impact of air frying, as an alternative to deep frying, on the quality and crust characteristics of French fries. In addition, differences in heat and mass transfer efficiency between air and deep frying were analyzed using the effective moisture diffusion coefficient (Deff), microstructural observations, and crust characteristics. The results indicated that compared with the oil content of 44.8 % obtained from deep frying, air frying (140 °C) for 5–25 min resulted in an oil content of ∼1.2 %. Furthermore, fries air fried for 20 min exhibited comparable moisture content (54.14 %), color, and texture to deep-fried (180 °C, 4 min) fries. Further analysis revealed that although the Deff during deep frying was five times higher than that during air frying (1.7184 × 10−7 m2/s), no significant difference was observed in the crust characteristics between the two frying methods. Consequently, these findings provide valuable insights to inform the development of effective strategies for substituting deep frying with air frying during the preparation of low-fat fried potato products.
炸薯条在全球不同人群中的广泛消费,因其高含油量引起的显著健康担忧而受到抑制。本研究调查了空气油炸作为油炸的替代品,对炸薯条的质量和脆皮特性的影响。此外,利用有效水分扩散系数(Deff)、微观结构观察和地壳特征分析了空气和油炸的传热传质效率差异。结果表明,与油炸获得的44.8%的含油量相比,空气油炸(140°C) 5-25分钟的含油量为~ 1.2%。此外,空气油炸20分钟的薯条显示出与油炸(180°C, 4分钟)薯条相当的水分含量(54.14%),颜色和质地。进一步的分析表明,虽然油炸时的脆度是空气油炸时的5倍(1.7184 × 10−7 m2/s),但两种油炸方式的脆度特征没有显著差异。因此,这些发现提供了有价值的见解,为制定在制备低脂油炸土豆产品时用空气油炸代替油炸的有效策略提供了信息。
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引用次数: 0
Preparation of a novel corn gluten meal hydrolysate against Helicobacter pylori: Structural characterization, screening and anti-adhesive mechanism analysis 新型抗幽门螺杆菌玉米蛋白粉水解产物的制备:结构表征、筛选及抗粘接机理分析
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119061
Guanlong Li , Yongchao Xie , Quanxin Wang , Zhengfei Miao , Zhe Wang , Xiaolan Liu , Xiqun Zheng
In recent years, the number of people infected with Helicobacter pylori has increased rapidly, which has become a serious public health problem. Although the effect of antibiotic treatment is remarkable, the emergence of strain resistance and side effects associated with antibiotic use highlight the need to develop safe and novel adjunctive methods for the prevention and treatment of H. pylori. In this study, a novel corn gluten meal hydrolysate with high anti-adhesive activity against H. pylori and peptide recovery was prepared. First, the corn gluten meal was hydrolyzed with Neutrase, and the resulting Neutrase hydrolysate (CGMH1) and precipitate were collected via centrifugation. Then, the precipitate was used as raw material and further hydrolyzed with Alcalase to yield the hydrolysate (CGMH2). The combination of CGMH1 and CGMH2 yielded the hydrolysate (CGMH4), which exhibits potent anti-adhesive activity against H. pylori and high peptide recovery. The anti-adhesive activity and peptide recovery rate of CGMH4 were 45.86 ± 0.35 % and 72.01 %, respectively, which lays a solid technical foundation for the subsequent development of CGMH4-based products. Superdex peptide gel chromatographic separation of CGMH4 revealed that fractions less than 1 kDa had the best anti-adhesive activity. Based on the affinity specificity between peptides and H. pylori adhesins, 207 peptide sequences with potential anti-adhesive effects against H. pylori were isolated and identified from the less than 1 kDa fraction. Through the physicochemical properties of the identified peptides combined with molecular docking analysis, nine peptide sequences were further screened. Visual analysis results demonstrated that the binding energy between the screened peptides and adhesins was stronger, with hydrogen bonds and hydrophobic interactions serving as the primary driving forces for their binding. In addition, the screened peptides mainly bind to the active center of H. pylori adhesins and inhibit the interaction between these adhesins and gastric mucosal epithelial cell receptors via occupying the active site, which constitutes the primary mechanism underlying the anti-adhesive activity of CGMH4. This study provides a new strategy for the prevention and treatment of H. pylori infection.
近年来,幽门螺杆菌感染人数迅速增加,已成为严重的公共卫生问题。尽管抗生素治疗的效果是显著的,但菌株耐药性的出现和与抗生素使用相关的副作用突出表明需要开发安全和新颖的辅助方法来预防和治疗幽门螺杆菌。本研究制备了一种具有高抗幽门螺杆菌粘附活性和肽回收率的新型玉米蛋白粉水解物。首先用Neutrase对玉米蛋白粉进行水解,离心收集所得的Neutrase水解产物(CGMH1)和沉淀。然后以沉淀为原料,用Alcalase进一步水解得到水解产物(CGMH2)。CGMH1和CGMH2结合得到的水解产物(CGMH4)具有较强的抗幽门螺杆菌粘附活性和较高的肽回收率。CGMH4的抗黏附活性和肽回收率分别为45.86±0.35%和72.01%,为后续开发基于CGMH4的产品奠定了坚实的技术基础。CGMH4的Superdex肽凝胶层析表明,小于1 kDa的组分具有最佳的抗粘附活性。基于肽段与幽门螺杆菌粘附素的亲和特异性,从小于1 kDa的片段中分离鉴定出207条具有潜在抗幽门螺杆菌粘附作用的肽段序列。通过对鉴定肽的理化性质结合分子对接分析,进一步筛选出9个肽序列。可视化分析结果表明,筛选的肽与粘附素之间的结合能更强,氢键和疏水相互作用是其结合的主要驱动力。此外,筛选到的多肽主要结合到幽门螺杆菌黏附素的活性中心,通过占据活性位点抑制黏附素与胃粘膜上皮细胞受体的相互作用,这是CGMH4抗黏附活性的主要机制。本研究为预防和治疗幽门螺旋杆菌感染提供了新的策略。
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引用次数: 0
Fermented beverages enriched with quinoa or chestnut–persimmon: Nutritional, functional, and anti-nutritional assessment 富含藜麦或栗子-柿子的发酵饮料:营养、功能和抗营养评估
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119054
Giorgia Secchi , Adineh Tajmousavilangerudi , Chiara Viretto , Ali Zein Alabiden Tlais , Andrea Angeli , Urska Vrhovsek , Federico Brigante , Martina Moretton , Emanuela Betta , Iuliia Khomenko , Franco Biasioli , Pavel Solovyev , Luana Bontempo , Andrea Mancini , Elena Franciosi
In this study, chestnut-persimmon and quinoa flours were used to formulate non-dairy beverages (CNP and Q, respectively) fermented with a strain of Lacticaseibacillus casei. Fermentability (microbial counts and pH) and biochemical, polyphenolic, volatile, and nutritional profiles were evaluated using 1H NMR, UPLC-QqQ-MS/MS, and HS-SPME/GC-MS. Both matrices sustained high LAB viability and stable acidification, confirming their suitability as fermentation substrates. NMR spectroscopy revealed matrix-dependent metabolic shifts: CNP beverages retained higher sugar levels and accumulated methanol and scyllo-inositol, whereas Q beverages exhibited enhanced nucleotide release. Lactic and acetic acids increased in both beverages. Among the 21 phenolic compounds identified, chlorogenic acid isomers, mainly derived from black carrot, were predominant. Total polyphenols content remained above 80 % of baseline values, with greater stability observed in CNP, while Q retained a more diversified flavonoid profile. VOC analysis showed suppression of aldehydes in fermented CNP, associated with reduced grassy notes, whereas fermented Q accumulated lipid-derived alcohols and fatty acids, resulting in more pronounced earthy and fatty aromas. Fermentation reduced anti-nutritional factors and improved in vitro protein digestibility, particularly in CNP. Overall, these findings demonstrated that both beverages are highly fermentable, nutritionally advantageous, and compositionally distinct, offering gluten-free, nutrient-rich options with complementary functional properties.
本研究以栗子-柿子粉和藜麦粉为原料,用一株干酪乳杆菌发酵制成非乳饮料(CNP和Q)。利用1H NMR、UPLC-QqQ-MS/MS和HS-SPME/GC-MS对发酵性(微生物计数和pH值)、生化、多酚、挥发性和营养成分进行评估。这两种基质都保持了较高的LAB活力和稳定的酸化,证实了它们作为发酵底物的适用性。核磁共振波谱揭示了基质依赖性代谢变化:CNP饮料保留了更高的糖含量和积累的甲醇和三基肌醇,而Q饮料显示出增强的核苷酸释放。两种饮料中的乳酸和乙酸含量都有所增加。21种酚类化合物中以绿原酸同分异构体居多,主要来源于黑胡萝卜。总多酚含量保持在基线值的80%以上,在CNP中观察到更大的稳定性,而Q保留了更多样化的类黄酮谱。挥发性有机化合物分析表明,发酵的CNP中醛类物质受到抑制,草味减少,而发酵的Q积累了脂质衍生的醇和脂肪酸,导致更明显的泥土和脂肪香味。发酵降低了抗营养因子,提高了体外蛋白质消化率,特别是在CNP中。总的来说,这些发现表明这两种饮料都具有高度可发酵性,营养优势,成分独特,提供无麸质,营养丰富的选择,并具有互补的功能特性。
{"title":"Fermented beverages enriched with quinoa or chestnut–persimmon: Nutritional, functional, and anti-nutritional assessment","authors":"Giorgia Secchi ,&nbsp;Adineh Tajmousavilangerudi ,&nbsp;Chiara Viretto ,&nbsp;Ali Zein Alabiden Tlais ,&nbsp;Andrea Angeli ,&nbsp;Urska Vrhovsek ,&nbsp;Federico Brigante ,&nbsp;Martina Moretton ,&nbsp;Emanuela Betta ,&nbsp;Iuliia Khomenko ,&nbsp;Franco Biasioli ,&nbsp;Pavel Solovyev ,&nbsp;Luana Bontempo ,&nbsp;Andrea Mancini ,&nbsp;Elena Franciosi","doi":"10.1016/j.lwt.2026.119054","DOIUrl":"10.1016/j.lwt.2026.119054","url":null,"abstract":"<div><div>In this study, chestnut-persimmon and quinoa flours were used to formulate non-dairy beverages (CNP and Q, respectively) fermented with a strain of <em>Lacticaseibacillus casei</em>. Fermentability (microbial counts and pH) and biochemical, polyphenolic, volatile, and nutritional profiles were evaluated using <sup>1</sup>H NMR, UPLC-QqQ-MS/MS, and HS-SPME/GC-MS. Both matrices sustained high LAB viability and stable acidification, confirming their suitability as fermentation substrates. NMR spectroscopy revealed matrix-dependent metabolic shifts: CNP beverages retained higher sugar levels and accumulated methanol and scyllo-inositol, whereas Q beverages exhibited enhanced nucleotide release. Lactic and acetic acids increased in both beverages. Among the 21 phenolic compounds identified, chlorogenic acid isomers, mainly derived from black carrot, were predominant. Total polyphenols content remained above 80 % of baseline values, with greater stability observed in CNP, while Q retained a more diversified flavonoid profile. VOC analysis showed suppression of aldehydes in fermented CNP, associated with reduced grassy notes, whereas fermented Q accumulated lipid-derived alcohols and fatty acids, resulting in more pronounced earthy and fatty aromas. Fermentation reduced anti-nutritional factors and improved <em>in vitro</em> protein digestibility, particularly in CNP. Overall, these findings demonstrated that both beverages are highly fermentable, nutritionally advantageous, and compositionally distinct, offering gluten-free, nutrient-rich options with complementary functional properties.</div></div>","PeriodicalId":382,"journal":{"name":"LWT - Food Science and Technology","volume":"241 ","pages":"Article 119054"},"PeriodicalIF":6.6,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of proteases combined with heat treatment for protein stabilisation of white wines improves their foaming properties 利用蛋白酶结合热处理来稳定白葡萄酒的蛋白质,可以改善白葡萄酒的起泡性能
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119055
Nadia Gregori , Jordi Gombau , Arnau Just-Borràs , Dolores Pérez , Jose Maria Heras , Fernando Tarin , Francesca Fort , Joan Miquel Canals , Fernando Zamora
In this study we evaluate the effectiveness of combining protease treatment with heat treatment as an alternative method to bentonite fining for achieving protein stability in white wines. Our results demonstrate that protease treatment can achieve complete protein stability or, at least, substantially reduce the amount of bentonite needed in highly unstable varieties such as Muscat of Alexandria. This reduction or even elimination of the amount of bentonite required mitigates its adverse effects. In addition to its role in achieving protein stability, protease treatment significantly enhances both the foamability (HM) and foam stability (HS) of wines. Notably, these improvements persist even when protease treatment is followed by bentonite fining. These enhancements offer a considerable advantage in the production of sparkling wines, especially in regions or with grape varieties that inherently produce base wines with limited foaming potential. Protease treatment could therefore play a valuable role in optimising the sensory and textural qualities of sparkling wines, thereby expanding production possibilities particularly in regions or with grape varieties that typically yield base wines with limited foaming capacity.
在这项研究中,我们评估了将蛋白酶处理与热处理相结合的有效性,作为膨润土精制的替代方法,以实现白葡萄酒中的蛋白质稳定性。我们的研究结果表明,蛋白酶处理可以实现完全的蛋白质稳定性,或者至少可以大大减少在高度不稳定的品种(如亚历山大的马斯喀特)中所需的膨润土的数量。这种减少甚至消除所需的膨润土量减轻了其不利影响。除了在实现蛋白质稳定性方面的作用外,蛋白酶处理还显著提高了葡萄酒的起泡性(HM)和泡沫稳定性(HS)。值得注意的是,即使在蛋白酶处理之后进行膨润土精制,这些改进仍然存在。这些改进为起泡酒的生产提供了相当大的优势,特别是在那些生产起泡潜力有限的基础酒的地区或葡萄品种。因此,蛋白酶处理可以在优化起泡酒的感官和质地方面发挥重要作用,从而扩大生产可能性,特别是在起泡能力有限的基础酒的地区或葡萄品种。
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引用次数: 0
Insight into the influence of tamarind seed polysaccharides on the rheological, structural properties and digestibility of wheat flour 罗望子多糖对小麦粉流变学、结构特性和消化率影响的研究
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119025
Qi Cui , Haoming Sun , Haotian Yin , Junjie Yi , Huang Zhang , Xueming Xu , Yongshuai Ma
This study examined the effect of various concentrations of tamarind seed polysaccharides (TSP) on the structural and rheological properties, as well as the digestibility of wheat flour. Scanning electron microscopy images revealed key structural differences: the starch granules appeared smooth and spherical, embedded within a continuous gluten network without TSP. However, with the addition of TSP, the surface of starch granules became rough and irregularly polygonal, with threads and tangles appearing around them. Rheological measurements showed that increasing TSP concentration: the values of G′ and G″ increased, indicating enhanced dough strength and viscoelasticity. During creep-recovery testing, the maximum recovery decreased from 2.807 × 10−3 Pa−1 to 7.45 × 10−4 Pa−1, reducing the dough's ability to recover its shape after deformation. Furthermore, TSP addition increased the resistant starch (RS) content of the wheat flour, rising slightly from approximately 11.04 g/100 g–13.80 g/100 g. These findings demonstrate that TSP substantially modifies the physicochemical and structural properties of wheat dough. These changes particularly the improved dough strength and increased resistant starch content, suggest potential for TSP in developing food products with tailored textures and enhanced nutritional benefits.
研究了不同浓度的罗望子多糖(TSP)对小麦粉的结构、流变学特性和消化率的影响。扫描电镜图像显示了关键的结构差异:淀粉颗粒看起来光滑和球形,嵌入在一个连续的面筋网络中,没有TSP。然而,随着TSP的加入,淀粉颗粒表面变得粗糙且不规则多边形,周围出现丝状和缠结。流变学测试表明,随着TSP浓度的增加,G′和G″的值增加,表明面团强度和粘弹性增强。在蠕变恢复试验中,最大回复率从2.807 × 10−3 Pa−1下降到7.45 × 10−4 Pa−1,降低了面团变形后恢复形状的能力。此外,添加TSP提高了小麦粉的抗性淀粉(RS)含量,从11.04 g/100 g到13.80 g/100 g略有上升。这些结果表明,TSP实质上改变了小麦面团的物理化学和结构性质。这些变化,特别是面团强度的提高和抗性淀粉含量的增加,表明TSP在开发具有定制质地和增强营养价值的食品方面具有潜力。
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引用次数: 0
Effect of co-fermentation of Pediococcus pentosaceus LWQ1 sourdough and commercial yeast on the quality and flavor of Chinese steamed bread 戊sacpedococcus petosaceus LWQ1酵母与商品酵母共发酵对馒头品质和风味的影响
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119039
Liao Mao , Yongxin Liu , Ruiyan Zhang , Zhengli Guo , Na Ji , Lei Dai , Qingjie Sun , Tongjie Liu , Yang Li
Sourdough is a traditional starter for Chinese steamed bread (CSB), contributing to its quality and flavor. However, the microbial complexity of sourdough poses challenges for standardized production. Therefore, isolating dominant strains to develop defined starter cultures has become a key strategy, improving CSB quality, and ensuring flavor consistency. Pediococcus pentosaceus is one of the dominant lactic acid bacteria in sourdough, yet its application in CSB remains limited. In this study, the effects of sourdough co-fermented with commercial yeast on the quality and flavor of CSB were investigated. Compared with CSB prepared with commercial yeast alone, sourdough fermented with P. pentosaceus LWQ1 significantly increased the specific volume and the levels of immobilized and free water, without significant alterations in textural properties. Scanning electron microscopy and confocal laser scanning microscopy revealed that sourdough disrupted the continuity of the gluten network, resulting in increased dough expansion and specific volume. Flavor analysis identified 55 volatile compounds, with 1-nonen-3-one contributing most significantly to the flavor profile. Compared with CSB fermented with Limosilactobacillus reuteri, LWQ1-CSB exhibited a flavor profile more similar to the yeast-only control. This study provides a reference for the application of P. pentosaceus sourdough in CSB production.
酵母是中国馒头(CSB)的传统开胃菜,有助于其质量和风味。然而,酵母微生物的复杂性给标准化生产带来了挑战。因此,分离优势菌株开发确定的发酵剂已成为提高CSB质量和确保风味一致性的关键策略。戊糖Pediococcus penttosaceus是酵母中的优势乳酸菌之一,但其在CSB中的应用仍然有限。本研究研究了酵母与商品酵母共发酵对CSB品质和风味的影响。与单独用商品酵母制备的CSB相比,P. pentosaceus LWQ1发酵的酵母比体积显著增加,固定水和游离水含量显著增加,但结构性质没有明显变化。扫描电镜和共聚焦激光扫描显微镜显示,酵母破坏了面筋网络的连续性,导致面团膨胀和比容增加。风味分析鉴定出55种挥发性化合物,其中1-壬烯-3- 1对风味特征贡献最大。与罗伊氏干酪乳杆菌发酵的CSB相比,LWQ1-CSB的风味特征更接近于纯酵母对照。本研究为戊酸酵母在CSB生产中的应用提供了参考。
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引用次数: 0
Development of a new PMA-qPCR method for quantification of four viable lactic acid bacteria species based on novel molecular markers 基于新型分子标记的四种活性乳酸菌PMA-qPCR定量方法的建立
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.lwt.2026.119064
Jian Wu , Ling Chen , Xinyu Zhao , Jiyun Ling , Weipeng Guo , Meiqing Deng , Tingting Liu , Tingting Liang , Ning Yang , Huiyuan Chen , Qingping Wu , Yuwei Wu , Shuhong Zhang
The recovery and quantification of viable probiotic cells from food samples are essential for verifying product efficacy and quality. The traditional culture methods are time-consuming and laborious. In this study, 998 genomic sequences of LAB were analyzed and four novel species-specific molecular markers were mined for Limosilactobacillus reuteri, Lacticaseibacillus rhamnosus, Lacticaseibacillus paracasei, and Lactobacillus acidophilus. The specificity of the genetic markers was evaluated using 15 LAB species. A new Propidium Monoazide-qPCR (PMA-qPCR) assay was developed to quantify four viable LAB species. The optimal PMA conditions were 40 μmol/L with 30 min of light exposure, 20 μmol/L with 20 min, 30 μmol/L with 20 min, and 30 μmol/L with 25 min for L. reuteri, L. rhamnosus, L. paracasei, and L. acidophilus, respectively. Four quantitative standard curves were established, demonstrating a robust correlation (R2 > 0.99) with viable cell counts over an extensive concentration range (103–108 CFU/mL). The detection limits were 8.45 × 103 CFU/mL, 4.30 × 102 CFU/mL, 7.40 × 103 CFU/mL, and 1.32 × 102 CFU/mL of pure culture for L. reuteri, L. rhamnosus, L. paracasei, and L. acidophilus, respectively. In 59 actual probiotic products, there was no significant difference between the results of PMA-qPCR and the traditional culture method (P > 0.05). The assay can accurately quantify viable bacteria in probiotic products within approximately 3 h and holds great promise for quality-control applications.
食品样品中活菌细胞的回收和定量对验证产品的功效和质量至关重要。传统的培养方法费时费力。本研究分析了998个乳酸菌基因组序列,并为罗伊氏乳杆菌、鼠李糖乳杆菌、副干酪乳杆菌和嗜酸乳杆菌挖掘了4个新的种特异性分子标记。利用15个LAB物种对遗传标记的特异性进行了评价。建立了一种新的单叠氮丙啶- qpcr (PMA-qPCR)方法,定量测定了四种活菌。罗伊氏乳杆菌、鼠李糖乳杆菌、副干酪乳杆菌和嗜酸乳杆菌的最佳PMA条件分别为光照30 min 40 μmol/L、20 min 20 μmol/L、20 min 30 μmol/L和25 min 30 μmol/L。建立了四条定量标准曲线,表明在广泛的浓度范围(103-108 CFU/mL)内,活细胞数与标准曲线有很强的相关性(R2 > 0.99)。罗伊氏乳杆菌、鼠李糖乳杆菌、副干酪乳杆菌和嗜酸乳杆菌的纯培养检出限分别为8.45 × 103 CFU/mL、4.30 × 102 CFU/mL、7.40 × 103 CFU/mL和1.32 × 102 CFU/mL。在59种实际益生菌产品中,PMA-qPCR与传统培养法的结果无显著差异(P > 0.05)。该方法可以在大约3小时内准确地定量益生菌产品中的活菌,并在质量控制应用中具有很大的前景。
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引用次数: 0
Ultrasound-assisted enzymatic extraction, characteristics, and biological activities of Benincasa hispida peel polysaccharides and their metal complexes 超声辅助酶法提取黑豆皮多糖及其金属配合物的特性及生物活性研究
IF 6.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-17 DOI: 10.1016/j.lwt.2026.119044
Cui Yang , Ruizhan Chen , Shujun Luo , Dongxue Li , Helong Bai , Wenjing Wu , Yongtang Wang , Mingze Gong
As an abundant agricultural by-product, Benincasa hispida (BH) peels hold untapped potential as a polysaccharide source, yet their composition, functional properties, and modification strategies remain underinvestigated. In contrast, research on polysaccharide-metal complexes lacks systematic comparisons of metal ion-specific effects. In this study, an acidic polysaccharide (UE-BHPP-2) was obtained via an ultrasound-assisted enzymatic extraction (UE) method, enhanced yields by 316.57 % over traditional heat reflux extraction. Polysaccharide complexes with zinc (UE-BHPP-2-Zn), iron (UE-BHPP-2-Fe), and selenium (UE-BHPP-2-Se) were prepared, with metal contents of 12.09 ± 1.95, 92.30 ± 5.04, and 0.94 ± 0.04 mg/g, respectively. The results showed that the complexes exhibit metal-specific antioxidant and hypoglycemic activities. At a concentration of 5 mg/mL, the ⋅OH scavenging activity and α-amylase inhibitory activity of the UE-BHPP-2-Zn complex were increased by 17.0 % and 2.83 %, respectively, compared with those of UE-BHPP-2. Furthermore, the complexes demonstrated good potential for trace element delivery in simulated digestion. This study confirms that metal ion complexation serves as a highly efficient strategy for polysaccharide functionalization, thereby offering a novel approach to the development of bifunctional materials that integrate dual properties of biological activity and trace element supplementation capacity.
作为一种丰富的农业副产品,Benincasa hispida (BH)果皮作为多糖来源具有未开发的潜力,但其组成、功能特性和修饰策略仍未得到充分研究。相比之下,多糖-金属配合物的研究缺乏金属离子特异性效应的系统比较。在本研究中,通过超声辅助酶萃取(UE)法获得了一种酸性多糖(UE- bhpp -2),比传统的热回流提取法的产率提高了316.57%。制备了含锌(UE-BHPP-2-Zn)、铁(UE-BHPP-2-Fe)和硒(UE-BHPP-2-Se)的多糖配合物,其金属含量分别为12.09±1.95、92.30±5.04和0.94±0.04 mg/g。结果表明,该配合物具有金属特异性抗氧化和降糖活性。在5 mg/mL浓度下,与UE-BHPP-2复合物相比,UE-BHPP-2- zn复合物的⋅OH清除活性和α-淀粉酶抑制活性分别提高了17.0%和2.83%。此外,该配合物在模拟消化中表现出良好的微量元素输送潜力。本研究证实了金属离子络合是一种高效的多糖功能化策略,从而为开发具有生物活性和补充微量元素双重特性的双功能材料提供了一种新的途径。
{"title":"Ultrasound-assisted enzymatic extraction, characteristics, and biological activities of Benincasa hispida peel polysaccharides and their metal complexes","authors":"Cui Yang ,&nbsp;Ruizhan Chen ,&nbsp;Shujun Luo ,&nbsp;Dongxue Li ,&nbsp;Helong Bai ,&nbsp;Wenjing Wu ,&nbsp;Yongtang Wang ,&nbsp;Mingze Gong","doi":"10.1016/j.lwt.2026.119044","DOIUrl":"10.1016/j.lwt.2026.119044","url":null,"abstract":"<div><div>As an abundant agricultural by-product, <em>Benincasa hispida</em> (BH) peels hold untapped potential as a polysaccharide source, yet their composition, functional properties, and modification strategies remain underinvestigated. In contrast, research on polysaccharide-metal complexes lacks systematic comparisons of metal ion-specific effects. In this study, an acidic polysaccharide (UE-BHPP-2) was obtained via an ultrasound-assisted enzymatic extraction (UE) method, enhanced yields by 316.57 % over traditional heat reflux extraction. Polysaccharide complexes with zinc (UE-BHPP-2-Zn), iron (UE-BHPP-2-Fe), and selenium (UE-BHPP-2-Se) were prepared, with metal contents of 12.09 ± 1.95, 92.30 ± 5.04, and 0.94 ± 0.04 mg/g, respectively. The results showed that the complexes exhibit metal-specific antioxidant and hypoglycemic activities. At a concentration of 5 mg/mL, the ⋅OH scavenging activity and α-amylase inhibitory activity of the UE-BHPP-2-Zn complex were increased by 17.0 % and 2.83 %, respectively, compared with those of UE-BHPP-2. Furthermore, the complexes demonstrated good potential for trace element delivery in simulated digestion. This study confirms that metal ion complexation serves as a highly efficient strategy for polysaccharide functionalization, thereby offering a novel approach to the development of bifunctional materials that integrate dual properties of biological activity and trace element supplementation capacity.</div></div>","PeriodicalId":382,"journal":{"name":"LWT - Food Science and Technology","volume":"241 ","pages":"Article 119044"},"PeriodicalIF":6.6,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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LWT - Food Science and Technology
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