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Cleaning and Sanitizing in Produce Facilities: Identifying Compliance Gaps and Associated Training Needs, Opportunities and Preferences 生产设施的清洁和消毒:识别合规差距和相关的培训需求、机会和偏好
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-23-011
J. Kovacevic
Inspections of fresh produce operations for compliance with the Produce Safety Rule (PSR) have identified cleaning and sanitizing (C/S) as a significant challenge. To better understand C/S practices in the produce industry a national survey was administered over a 3-week period in summer 2020 to fresh produce operations in U.S. Survey responses (n = 162) represented 135 produce operations from 18 different states. The most common materials reported for harvesting containers (n = 50 responses) were plastic (80%), wood (30%), cardboard (16%), and canvas (12%), while stainless steel (81%) and plastic (71%) were most commonly used for post-harvest equipment (n = 42 responses). Bleach (40/63), quaternary ammonium compounds (32/63), and peracetic acid (26/63) were the most commonly reported sanitizers. Respondents indicated there is a need for resources on principles and practical implementation of C/S, identification of hazards and prioritizing C/S activities, establishing a C/S program, and verification of C/S effectiveness, in English, Spanish and other languages. High turnover and seasonal workforce (37/65) and no time to C/S (19/65) were indicated as major barriers for improving C/S in a facility. The results of the survey helped guide a virtual C/S workshop in 2021, and an in-person workshop currently in development.
对新鲜农产品操作是否符合《农产品安全规则》(PSR)的检查已将清洁和消毒(C/S)确定为一项重大挑战。为了更好地了解农产品行业的C/S实践,我们在2020年夏季对美国的新鲜农产品运营进行了为期3周的全国性调查。调查回复(n = 162)代表了来自18个不同州的135家生产运营。收获容器最常用的材料(n = 50份回复)是塑料(80%)、木材(30%)、纸板(16%)和帆布(12%),而不锈钢(81%)和塑料(71%)最常用于收获后设备(n = 42份回复)。漂白剂(40/63)、季铵化合物(32/63)和过氧乙酸(26/63)是最常见的消毒剂。受访者表示,需要以英语、西班牙语和其他语言提供关于C/S的原则和实际实施、识别危害和优先安排C/S活动、建立C/S计划和验证C/S有效性的资源。高流失率和季节性劳动力(37/65)以及没有时间达到C/S(19/65)被认为是改善设施C/S的主要障碍。调查结果有助于指导2021年的虚拟C/S研讨会,以及目前正在开发的现场研讨会。
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引用次数: 1
Combined Effects of Sanitizers and UV-C Light on Listeria monocytogenes Biofilm Growth and Survivability on Produce-Harvesting Materials Used in the Tree Fruit Production Industry 杀菌剂和UV-C光对果树生产用采收材料上单核细胞增生李斯特菌生物膜生长和存活的联合影响
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-22-037
V. Trinetta
Listeria monocytogenes is an aggressive biofilm former that can establish and persist in food processing environments. Commonly associated with ready-to-eat and dairy products, this pathogenic bacterium has recently been increasingly linked to fresh produce outbreaks. Equipment used during harvesting and handling of produce can provide a niche environment for biofilm growth and persistence. Based on a survey conducted among stakeholders in the tree fruit production industry, three favored materials for storing and harvesting produce were identified: nylon, wood, and plastic. The purpose of this study was to investigate the application of the generally recognized as safe sanitizers lactic acid, thymol, and silver citric acid (SDC) and UV-C light alone or in combination for 2 or 5 min on different food-contact surfaces used during tree fruit harvesting and storing. Multistrain L. monocytogenes biofilms were grown in a Centers for Disease Control and Prevention biofilm reactor for 96 h on wood, nylon, and polycarbonate coupons at 20 ± 2°C. After each treatment, coupons were neutralized and the remaining cells were enumerated. Results showed that the most effective treatment was the simultaneous use of UV-C light and SDC (4-log reduction) and that the least effective treatment was UV-C light alone (P < 0.05). The type of material was found to play a significant role in the efficacy of the sanitizers (P < 0.05). This study demonstrates the ability of L. monocytogenes to grow and form biofilms on different surfaces and contributes to an understanding of the response of this food safety threat against antimicrobial intervention strategies.
单核增生李斯特菌是一种具有侵袭性的生物膜前体,可以在食品加工环境中建立并持续存在。这种致病菌通常与即食食品和乳制品有关,最近与新鲜农产品爆发的联系越来越紧密。在收获和处理农产品过程中使用的设备可以为生物膜的生长和持久性提供一个生态位环境。根据对果树生产行业的利益相关者进行的一项调查,确定了三种最受欢迎的储存和收获材料:尼龙、木材和塑料。本研究的目的是研究公认的安全杀菌剂乳酸、百里香酚和柠檬酸银(SDC)和UV-C光单独或联合在果树收获和储存过程中不同食物接触表面上的应用。在美国疾病控制与预防中心的生物膜反应器中,在木材、尼龙和聚碳酸酯片上,在20±2°C下培养96 h。每次处理后,将细胞券中和,并对剩余细胞进行计数。结果表明,UV-C光与SDC同时使用效果最佳(4-log降低),单独使用UV-C光效果最差(P < 0.05)。材料类型对消毒效果有显著影响(P < 0.05)。这项研究证明了单核增生乳杆菌在不同表面生长和形成生物膜的能力,并有助于了解这种食品安全威胁对抗菌干预策略的反应。
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引用次数: 0
Fresh Produce Harvesting Equipment – A Review of Cleaning and Sanitizing Practices and Related Science 新鲜农产品收获设备-清洁和消毒实践及相关科学综述
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-22-023
S. Leaman
During produce harvesting operations, various types of tools, equipment, and containers have direct contact with crops. Best practices for harvesting equipment include routine cleaning and sanitation of food-contact surfaces and areas adjacent to food-contact surfaces. Studies investigating transfer of human pathogens to produce during harvesting operations have revealed numerous crop-contact points with the potential to serve as conduits for pathogen transfer, including harvesting machinery, knives, conveyors, cutting boards, harvest bins and cartons, and cleaning equipment. When these surfaces are contaminated with human pathogens, the pathogens can be transferred to crops during harvesting activities. Minimizing and controlling microbial hazards to prevent contamination of produce during harvesting operations in both indoor and outdoor settings presents challenges that require a transformative level of risk awareness and vigilance from all involved in management and operations. Although new technologies are being explored to improve equipment cleanability, prevention combined with robust cleaning and sanitizing methods remain the most critical maintenance aspects of harvesting equipment that is at low risk for contamination. As part of the produce industry’s continuous efforts to enhance the safety of harvested fresh produce crops, this review summarizes scientific findings that harvesting equipment operators can utilize to evaluate and further inform current cleaning and sanitation practices.
在农产品收割作业中,各种类型的工具、设备和容器与作物直接接触。收割设备的最佳做法包括对食物接触表面和食物接触表面附近区域进行常规清洁和卫生。调查人类病原体在收割过程中转移到农产品的研究表明,许多作物接触点有可能成为病原体转移的管道,包括收割机械、刀具、输送机、砧板、收割箱和纸箱以及清洁设备。当这些表面被人类病原体污染时,病原体可以在收割活动中转移到作物上。在室内和室外环境中,最大限度地减少和控制微生物危害,以防止农产品在收获操作过程中受到污染,这是一项挑战,需要所有参与管理和操作的人员具有变革性的风险意识和警惕性。尽管正在探索新技术来提高设备的可清洁性,但预防与强有力的清洁和消毒方法相结合仍然是低污染风险收割设备最关键的维护方面。作为农产品行业不断努力提高收获的新鲜农产品作物安全性的一部分,本综述总结了收获设备操作员可以用来评估和进一步告知当前清洁和卫生做法的科学发现。
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引用次数: 0
Salmonella Prevalence is Low in Deep Tissue Lymph Nodes of Hog Carcasses from a Pork Processing Plant in Alberta, Canada 沙门氏菌在加拿大阿尔伯塔省一家猪肉加工厂的猪尸体深层组织淋巴结中的流行率很低
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-23-005
D. Holman
Deep tissue lymph nodes (DTLNs) could be an important source of Salmonella in pork because carcass decontamination strategies have no effect on Salmonella cells that are deeply embedded and protected. The objective of this study was to determine the prevalence, concentration, and antimicrobial resistance of Salmonella in DTLNs in chilled hog carcasses as well as in ground pork. A total of 400 DTLNs were collected over a 10-month period from a commercial pork processing plant. Salmonella was detected in 2 (0.5%) of 400 DTLNs; Salmonella Uganda was detected in a DTLN from the belly and Salmonella Bovismorbificans in one DTLN from the shoulder. Salmonella Uganda was also detected in one ground pork sample. The three Salmonella isolates were susceptible to all antimicrobials tested, and no clinically significant antimicrobial resistance genes were detected in these genomes after sequencing. The prevalence of Salmonella in DTLNs in pork tissues intended for human consumption is very low and could be a minor source of contamination in the production of ground pork. These findings are important for the pork industry to assess the risks and benefits of removing DTLNs from pork cuts and trimmings.
深层组织淋巴结(DTLNs)可能是猪肉中沙门氏菌的重要来源,因为胴体去污策略对深埋和受保护的沙门氏菌细胞没有影响。本研究的目的是确定冷藏猪胴体和绞肉中DTLNs中沙门氏菌的流行率、浓度和耐药性。在10个月的时间里,从一家商业猪肉加工厂总共收集了400个DTLNs。400个DTLNs中有2个(0.5%)检出沙门氏菌;在腹部的一个DTLN中检测到乌干达沙门氏菌,在肩部的一个DTAN中检测到牛源性沙门氏菌。乌干达沙门氏菌也在一份猪肉样品中被检测到。三个沙门氏菌分离株对所有测试的抗菌药物都敏感,测序后在这些基因组中没有检测到具有临床意义的抗菌耐药性基因。供人类食用的猪肉组织中的DTLNs中沙门氏菌的流行率非常低,可能是绞肉生产中的一个次要污染源。这些发现对于猪肉行业评估从猪肉切片和辅料中去除DTLNs的风险和益处非常重要。
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引用次数: 0
Identifying Predictors of Safe Food Handling Practices among Canadian Households with Children Under Eighteen Years 在加拿大有18岁以下儿童的家庭中确定安全食品处理实践的预测因素
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-22-039
David Obande
Poor food handling practices at home are a common cause of foodborne illness. Children are more susceptible to foodborne illness than adults. Because children’s food safety depends on the safe food handling practices of parents and caregivers, this study aims to identify determinants of safe food handling practices among Canadian families with children under 18 years. Data for Canadian households with children (n = 294) were extracted from a larger telephone survey conducted across all Canadian provinces and territories between 2014 and 2015. Four food safety practice outcomes and six demographic variables were examined using multivariable logistics regression. Most survey participants were females (56%) who had less than a bachelor’s degree (67%) and were caring for one child (55%). Approximately 90% of caregivers reported proper hand hygiene, and 79% refrigerated leftovers within 2 h of cooking. Only 33% of caregivers reported preventing cross-contamination, and fewer reported using food thermometers for poultry cuts (13%) and hamburgers (11%). Those in the higher income and education categories were less likely to follow safe food handling practices such as hand hygiene and safe refrigeration of leftovers. This research highlights the need for food safety interventions that target Canadian families with children within certain demographic groups.
家里处理食物的方法不好是引起食源性疾病的常见原因。儿童比成年人更容易患食源性疾病。由于儿童的食品安全取决于父母和照顾者的安全食品处理做法,本研究旨在确定加拿大有18岁以下儿童的家庭中安全食品处理行为的决定因素。加拿大有孩子家庭(n=294)的数据来自2014年至2015年间在加拿大所有省份和地区进行的一项更大规模的电话调查。使用多变量物流回归检验了四个食品安全实践结果和六个人口统计学变量。大多数调查参与者是女性(56%),她们没有学士学位(67%),并且正在照顾一个孩子(55%)。大约90%的护理人员表示手部卫生良好,79%的护理人员在烹饪后2小时内将剩菜冷藏。只有33%的护理人员报告说他们防止了交叉污染,很少有人报告说他们在家禽切块(13%)和汉堡(11%)中使用了食品温度计。那些收入较高和受教育程度较高的人不太可能遵循安全的食品处理做法,如手部卫生和剩菜的安全冷藏。这项研究强调了针对特定人口群体中有孩子的加拿大家庭的食品安全干预措施的必要性。
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引用次数: 0
Produce Safety Alliance Training: Long-Term Behavioral Change Study in the North Central Region 农产品安全联盟培训:中北部地区长期行为改变研究
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-034
Arlene E. Enderton
Starting in 2016, the Produce Safety Alliance (PSA) Grower Training has been offered as the only Food and Drug Administration–approved course to meet the Food Safety Modernization Act Produce Safety Rule educational requirements for produce growers. This study interviewed farmers four to six years after training to measure changes made. Other studies have measured change one year after training. The most common types of changes this study’s participants made were in the areas of health and hygiene and preventing cross-contamination. These changes did not differ by farm size. The authors expected farmers would have made more difficult changes, given the greater amount of time between taking the training and the study. However, the greatest barriers to making food safety changes—limited time, money, and labor availability—persisted for study participants. This study also found that human capital was the most effective resource that helped farms make food safety changes. Recommendations based on the study include prioritizing states' federally funded Cooperative Agreement Program *Author for correspondence: Phone: +1 319.559.2193; Email: arlene@iastate.edu (CAP) funds to pay for staff, taking advantage of the three ways to offer the PSA Grower Training, and investing in ways to help overcome the main barriers that growers identified: time, money, and labor constraints.
从2016年开始,农产品安全联盟(PSA)种植者培训已成为食品和药物管理局批准的唯一课程,以满足《食品安全现代化法案》对农产品种植者的生产安全规则教育要求。这项研究采访了培训后4到6年的农民,以衡量所取得的变化。其他研究测量了训练一年后的变化。这项研究的参与者所做的最常见的改变是在健康和卫生以及防止交叉污染方面。这些变化没有因农场规模而异。这组作者预计,考虑到接受培训和进行研究之间的时间间隔更长,农民会做出更困难的改变。然而,对研究参与者来说,做出食品安全改变的最大障碍是有限的时间、金钱和劳动力可用性。该研究还发现,人力资本是帮助农场进行食品安全变革的最有效资源。基于该研究的建议包括优先考虑各州联邦资助的合作协议计划*通信作者:电话:+1 319.559.2193;电子邮件:arlene@iastate.edu (CAP)资金支付员工,利用提供PSA种植者培训的三种方式,并投资于帮助种植者克服主要障碍的方法:时间、金钱和劳动力限制。
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引用次数: 0
Characterization of Escherichia coli Isolates from Agricultural Water on Kansas and Missouri Fresh Produce Farms by Whole-Genome Sequencing 堪萨斯州和密苏里州新鲜农产品农场农业用水大肠杆菌全基因组测序鉴定
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-038
V. Trinetta
Contaminated agricultural water has been a source of pathogenic Escherichia coli in recent produce-related outbreaks. The purpose of this study was to characterize E. coli isolates from agricultural water sources by using whole-genome sequencing (WGS) to better understand contamination routes. Groundwater and surface water samples were collected quarterly from five farms in Missouri and Kansas over a 1-year period. Samples were tested for generic E. coli by using U.S. Environmental Protection Agency Method 1603, and presumptive E. coli colonies were isolated. In total, 570 isolates were analyzed by PCR, with 191 of these isolates confirmed as E. coli. WGS was completed using an Illumina MiSeq system. The de novo genome assemblies were obtained with Shovill pipeline version 0.9. The NCBI Pathogen Detection system was used to identify antimicrobial resistance (AMR) genes. The prevalence of E. coli was higher during spring and summer than winter. A diverse serotype pool was observed where more than 53% of isolates could be linked to a bovine source as the potential animal host. An AMR analysis showed that 100% of isolates carried at least two antimicrobial resistance genes. Recognizing the diversity of E. coli may help guide agricultural water assessments as proposed in the new agricultural water rule Food Safety Modernization Act Produce Safety Rule.
在最近与农产品相关的疫情中,受污染的农业用水一直是致病性大肠杆菌的来源。本研究的目的是通过使用全基因组测序(WGS)来表征来自农业水源的大肠杆菌分离株,以更好地了解污染途径。在一年的时间里,每季度从密苏里州和堪萨斯州的五个农场采集地下水和地表水样本。使用美国环境保护局方法1603对样品进行通用大肠杆菌检测,并分离出推定大肠杆菌菌落。总共对570个分离株进行了PCR分析,其中191个分离株被确认为大肠杆菌。WGS是使用Illumina MiSeq系统完成的。使用Shovill管道版本0.9获得从头基因组组装。NCBI病原体检测系统用于鉴定抗微生物耐药性(AMR)基因。春季和夏季大肠杆菌的流行率高于冬季。观察到一个不同的血清型库,其中超过53%的分离株可能与作为潜在动物宿主的牛源有关。AMR分析显示,100%的分离株携带至少两个抗微生物耐药性基因。认识到大肠杆菌的多样性可能有助于指导新的农业用水规则《食品安全现代化法案》《生产安全规则》中提出的农业用水评估。
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引用次数: 0
Increasing Food Safety Preparedness of Small and Emerging Food Businesses with Targeted Food Safety Training 通过有针对性的食品安全培训提高小型和新兴食品企业的食品安全准备
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-031
A. Kinchla
There is strong consumer demand for local, value- added food products; however, these smaller scale food manufacturing operations tend to face more challenges in understanding and complying with food safety regulations, specifically, the Food Safety Modernization Act (FSMA) Preventive Controls for Human Food (PCHF) rule. An eight- module educational program was developed to introduce food safety throughout the product development life cycle and included concepts related to the FSMA PCHF rule. The program was offered in person (n = 2) and virtually (n = 6) to 143 participants in total, who were mostly early-stage food developers. Most (74%, n = 90) had little to no prior food safety-related training and experience. The majority (93%, n = 90) agreed they will apply the knowledge and skills learned in the course to food applications, such as developing food safety plans, label review, and good manufacturing practices. Self-rated level understanding for each module ranged from 4.4 to 4.6 on a 5-point Likert scale. Overall, participants felt better prepared to conduct various food safety activities after taking the course, supporting the need for non-mandatory training opportunities to enhance FSMA PCHF regulatory compliance. These types of training may be important for small-scale operations to improve the learning outcome and regulatory compliance.
消费者对本地增值食品有着强烈的需求;然而,这些规模较小的食品生产企业在理解和遵守食品安全法规,特别是《食品安全现代化法案》(FSMA)《人类食品预防控制》(PCHF)规则方面往往面临更多挑战。制定了一个八模块的教育计划,在整个产品开发生命周期中介绍食品安全,并包括与FSMA PCHF规则相关的概念。该项目由143名参与者亲自(n=2)和虚拟(n=6)参与,他们大多是早期食品开发人员。大多数人(74%,n=90)以前几乎没有或根本没有食品安全相关的培训和经验。大多数人(93%,n=90)同意将在课程中学到的知识和技能应用于食品应用,如制定食品安全计划、标签审查和良好生产实践。每个模块的自评水平理解在5分Likert量表上从4.4到4.6不等。总的来说,参与者在参加课程后感觉更好地准备好进行各种食品安全活动,这支持了非强制性培训机会的必要性,以提高FSMA PCHF的监管合规性。这些类型的培训对于小规模运营可能很重要,以提高学习成果和法规遵从性。
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引用次数: 0
Exploring Young Women’s Perceptions of Their Food Skills 探索年轻女性对自己饮食技巧的认知
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-032
J. Matthews
Many young adults mistakenly perceive that they have good food safety knowledge and are unlikely to experience foodborne illness. Young women’s food skills are of partic- ular importance because women are responsible for most food-related tasks in the home and many children learn food skills from their mothers. This descriptive qualitative study explored young women’s perceptions of food skills in three domains: food selection and planning, food prepara- tion, and food safety and storage. Through individual inter- views, 30 young women aged 17 to 30 years answered the following three key research questions: (i) What do food skills mean to you? (ii) How did you learn them? and (iii) In what areas are you most and least confident? Few participants mentioned food safety in their top-of- mind definition of food skills. More than half were least confident in the domain of food safety and storage. Fear prompted avoidance of cooking meat – even by those who were not vegan or vegetarian. Food skill interventions or curricula should emphasize food safety and storage so that young adults can reap the dietary and financial benefits of preparing all types of food. Consistent with others’ recom- mendations, the two most important food safety topics for educating young adults should be (i) cross-contamination and sanitation procedures and (ii) safe times and tempera- tures for cooking or storing food.
许多年轻人错误地认为他们有良好的食品安全知识,不太可能经历食源性疾病。年轻妇女的饮食技巧尤其重要,因为妇女在家里负责大多数与食物有关的工作,许多孩子从母亲那里学习饮食技巧。这项描述性定性研究探讨了年轻女性在三个领域对食品技能的看法:食品选择和规划,食品制备,食品安全和储存。通过个人访谈,30名年龄在17至30岁之间的年轻女性回答了以下三个关键研究问题:(i)食物技能对你意味着什么?(ii)你是怎么学会的?(3)你在哪些方面最自信,哪些方面最不自信?很少有参与者提到食品安全是他们对食品技能的首要定义。超过一半的人对食品安全和储存领域最缺乏信心。恐惧促使人们避免烹饪肉类——即使是那些不是素食主义者或素食主义者。食品技能干预措施或课程应强调食品安全和储存,以便年轻人能够从准备各种食品中获得饮食和经济上的好处。与其他人的建议一致,教育年轻人的两个最重要的食品安全主题应该是(i)交叉污染和卫生程序以及(ii)烹饪或储存食物的安全时间和温度。
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引用次数: 0
Risk Management Options to Reduce Human Salmonellosis Cases Due to Consumption of Raw Poultry 减少因食用生禽导致的人类沙门氏菌病病例的风险管理选择
Q4 Medicine Pub Date : 2023-03-01 DOI: 10.4315/fpt-22-035
A. Cohn
Salmonella cases due to cross-contamination by, or consumption of, raw poultry continue to be a major public health concern. Processors have yet to identify an effective “kill step” in raw poultry production, and food safety interventions may target many compartments of the supply chain, from breeder and grandparent flocks to consumer cooking practices, complicating the prioritization of specific areas to effectively manage risk. Moreover, raw poultry can be contaminated by diverse Salmonella serovars, ranging from multidrug resistant Salmonella Infantis to pansusceptible Salmonella Kentucky sequence type 152, which has substantially reduced likelihood of causing human disease. “Farm-to-table” risk models help assess the public health impact of different Salmonella risk management strategies and thereby inform policy priorities. This article provides an overview of risk management practices that should be considered and evaluated in Salmonella risk assessments, including risk management strategies focusing on (i) preharvest; (ii) slaughter and further processing; (iii) consumer product handling; and (iv) regulatory approaches. Data and model needs to allow assessment of these risk management strategies are also discussed. The information presented here represents a critical step in ensuring that future Salmonella risk assessment and risk management efforts represent a comprehensive systems approach and consider all potential options for Salmonella risk reduction.
由生家禽交叉污染或食用生家禽引起的沙门氏菌病例仍然是一个主要的公共卫生问题。加工商尚未确定生禽生产中有效的“屠宰步骤”,食品安全干预措施可能针对供应链的许多环节,从饲养员和祖辈鸡群到消费者的烹饪做法,这使确定特定领域的优先级以有效管理风险复杂化。此外,生家禽可能受到多种沙门氏菌血清型的污染,从耐多药的婴儿沙门氏菌到全敏感的肯塔基沙门氏菌序列152型,这大大降低了引起人类疾病的可能性。“从农场到餐桌”风险模型有助于评估不同沙门氏菌风险管理战略对公共卫生的影响,从而为政策重点提供信息。本文概述了沙门氏菌风险评估中应考虑和评估的风险管理实践,包括侧重于(1)收获前的风险管理策略;(ii)屠宰及进一步加工;(iii)消费品处理;(四)监管方法。本文还讨论了评估这些风险管理策略所需的数据和模型。这里提供的信息是确保未来沙门氏菌风险评估和风险管理工作的关键一步,代表了一种全面的系统方法,并考虑了减少沙门氏菌风险的所有潜在选择。
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引用次数: 0
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