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Salmonella Prevalence is Low in Deep Tissue Lymph Nodes of Hog Carcasses from a Pork Processing Plant in Alberta, Canada 沙门氏菌在加拿大阿尔伯塔省一家猪肉加工厂的猪尸体深层组织淋巴结中的流行率很低
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-23-005
D. Holman
Deep tissue lymph nodes (DTLNs) could be an important source of Salmonella in pork because carcass decontamination strategies have no effect on Salmonella cells that are deeply embedded and protected. The objective of this study was to determine the prevalence, concentration, and antimicrobial resistance of Salmonella in DTLNs in chilled hog carcasses as well as in ground pork. A total of 400 DTLNs were collected over a 10-month period from a commercial pork processing plant. Salmonella was detected in 2 (0.5%) of 400 DTLNs; Salmonella Uganda was detected in a DTLN from the belly and Salmonella Bovismorbificans in one DTLN from the shoulder. Salmonella Uganda was also detected in one ground pork sample. The three Salmonella isolates were susceptible to all antimicrobials tested, and no clinically significant antimicrobial resistance genes were detected in these genomes after sequencing. The prevalence of Salmonella in DTLNs in pork tissues intended for human consumption is very low and could be a minor source of contamination in the production of ground pork. These findings are important for the pork industry to assess the risks and benefits of removing DTLNs from pork cuts and trimmings.
深层组织淋巴结(DTLNs)可能是猪肉中沙门氏菌的重要来源,因为胴体去污策略对深埋和受保护的沙门氏菌细胞没有影响。本研究的目的是确定冷藏猪胴体和绞肉中DTLNs中沙门氏菌的流行率、浓度和耐药性。在10个月的时间里,从一家商业猪肉加工厂总共收集了400个DTLNs。400个DTLNs中有2个(0.5%)检出沙门氏菌;在腹部的一个DTLN中检测到乌干达沙门氏菌,在肩部的一个DTAN中检测到牛源性沙门氏菌。乌干达沙门氏菌也在一份猪肉样品中被检测到。三个沙门氏菌分离株对所有测试的抗菌药物都敏感,测序后在这些基因组中没有检测到具有临床意义的抗菌耐药性基因。供人类食用的猪肉组织中的DTLNs中沙门氏菌的流行率非常低,可能是绞肉生产中的一个次要污染源。这些发现对于猪肉行业评估从猪肉切片和辅料中去除DTLNs的风险和益处非常重要。
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引用次数: 0
Identifying Predictors of Safe Food Handling Practices among Canadian Households with Children Under Eighteen Years 在加拿大有18岁以下儿童的家庭中确定安全食品处理实践的预测因素
Q4 Medicine Pub Date : 2023-05-01 DOI: 10.4315/fpt-22-039
David Obande
Poor food handling practices at home are a common cause of foodborne illness. Children are more susceptible to foodborne illness than adults. Because children’s food safety depends on the safe food handling practices of parents and caregivers, this study aims to identify determinants of safe food handling practices among Canadian families with children under 18 years. Data for Canadian households with children (n = 294) were extracted from a larger telephone survey conducted across all Canadian provinces and territories between 2014 and 2015. Four food safety practice outcomes and six demographic variables were examined using multivariable logistics regression. Most survey participants were females (56%) who had less than a bachelor’s degree (67%) and were caring for one child (55%). Approximately 90% of caregivers reported proper hand hygiene, and 79% refrigerated leftovers within 2 h of cooking. Only 33% of caregivers reported preventing cross-contamination, and fewer reported using food thermometers for poultry cuts (13%) and hamburgers (11%). Those in the higher income and education categories were less likely to follow safe food handling practices such as hand hygiene and safe refrigeration of leftovers. This research highlights the need for food safety interventions that target Canadian families with children within certain demographic groups.
家里处理食物的方法不好是引起食源性疾病的常见原因。儿童比成年人更容易患食源性疾病。由于儿童的食品安全取决于父母和照顾者的安全食品处理做法,本研究旨在确定加拿大有18岁以下儿童的家庭中安全食品处理行为的决定因素。加拿大有孩子家庭(n=294)的数据来自2014年至2015年间在加拿大所有省份和地区进行的一项更大规模的电话调查。使用多变量物流回归检验了四个食品安全实践结果和六个人口统计学变量。大多数调查参与者是女性(56%),她们没有学士学位(67%),并且正在照顾一个孩子(55%)。大约90%的护理人员表示手部卫生良好,79%的护理人员在烹饪后2小时内将剩菜冷藏。只有33%的护理人员报告说他们防止了交叉污染,很少有人报告说他们在家禽切块(13%)和汉堡(11%)中使用了食品温度计。那些收入较高和受教育程度较高的人不太可能遵循安全的食品处理做法,如手部卫生和剩菜的安全冷藏。这项研究强调了针对特定人口群体中有孩子的加拿大家庭的食品安全干预措施的必要性。
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引用次数: 0
Produce Safety Alliance Training: Long-Term Behavioral Change Study in the North Central Region 农产品安全联盟培训:中北部地区长期行为改变研究
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-034
Arlene E. Enderton
Starting in 2016, the Produce Safety Alliance (PSA) Grower Training has been offered as the only Food and Drug Administration–approved course to meet the Food Safety Modernization Act Produce Safety Rule educational requirements for produce growers. This study interviewed farmers four to six years after training to measure changes made. Other studies have measured change one year after training. The most common types of changes this study’s participants made were in the areas of health and hygiene and preventing cross-contamination. These changes did not differ by farm size. The authors expected farmers would have made more difficult changes, given the greater amount of time between taking the training and the study. However, the greatest barriers to making food safety changes—limited time, money, and labor availability—persisted for study participants. This study also found that human capital was the most effective resource that helped farms make food safety changes. Recommendations based on the study include prioritizing states' federally funded Cooperative Agreement Program *Author for correspondence: Phone: +1 319.559.2193; Email: arlene@iastate.edu (CAP) funds to pay for staff, taking advantage of the three ways to offer the PSA Grower Training, and investing in ways to help overcome the main barriers that growers identified: time, money, and labor constraints.
从2016年开始,农产品安全联盟(PSA)种植者培训已成为食品和药物管理局批准的唯一课程,以满足《食品安全现代化法案》对农产品种植者的生产安全规则教育要求。这项研究采访了培训后4到6年的农民,以衡量所取得的变化。其他研究测量了训练一年后的变化。这项研究的参与者所做的最常见的改变是在健康和卫生以及防止交叉污染方面。这些变化没有因农场规模而异。这组作者预计,考虑到接受培训和进行研究之间的时间间隔更长,农民会做出更困难的改变。然而,对研究参与者来说,做出食品安全改变的最大障碍是有限的时间、金钱和劳动力可用性。该研究还发现,人力资本是帮助农场进行食品安全变革的最有效资源。基于该研究的建议包括优先考虑各州联邦资助的合作协议计划*通信作者:电话:+1 319.559.2193;电子邮件:arlene@iastate.edu (CAP)资金支付员工,利用提供PSA种植者培训的三种方式,并投资于帮助种植者克服主要障碍的方法:时间、金钱和劳动力限制。
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引用次数: 0
Characterization of Escherichia coli Isolates from Agricultural Water on Kansas and Missouri Fresh Produce Farms by Whole-Genome Sequencing 堪萨斯州和密苏里州新鲜农产品农场农业用水大肠杆菌全基因组测序鉴定
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-038
V. Trinetta
Contaminated agricultural water has been a source of pathogenic Escherichia coli in recent produce-related outbreaks. The purpose of this study was to characterize E. coli isolates from agricultural water sources by using whole-genome sequencing (WGS) to better understand contamination routes. Groundwater and surface water samples were collected quarterly from five farms in Missouri and Kansas over a 1-year period. Samples were tested for generic E. coli by using U.S. Environmental Protection Agency Method 1603, and presumptive E. coli colonies were isolated. In total, 570 isolates were analyzed by PCR, with 191 of these isolates confirmed as E. coli. WGS was completed using an Illumina MiSeq system. The de novo genome assemblies were obtained with Shovill pipeline version 0.9. The NCBI Pathogen Detection system was used to identify antimicrobial resistance (AMR) genes. The prevalence of E. coli was higher during spring and summer than winter. A diverse serotype pool was observed where more than 53% of isolates could be linked to a bovine source as the potential animal host. An AMR analysis showed that 100% of isolates carried at least two antimicrobial resistance genes. Recognizing the diversity of E. coli may help guide agricultural water assessments as proposed in the new agricultural water rule Food Safety Modernization Act Produce Safety Rule.
在最近与农产品相关的疫情中,受污染的农业用水一直是致病性大肠杆菌的来源。本研究的目的是通过使用全基因组测序(WGS)来表征来自农业水源的大肠杆菌分离株,以更好地了解污染途径。在一年的时间里,每季度从密苏里州和堪萨斯州的五个农场采集地下水和地表水样本。使用美国环境保护局方法1603对样品进行通用大肠杆菌检测,并分离出推定大肠杆菌菌落。总共对570个分离株进行了PCR分析,其中191个分离株被确认为大肠杆菌。WGS是使用Illumina MiSeq系统完成的。使用Shovill管道版本0.9获得从头基因组组装。NCBI病原体检测系统用于鉴定抗微生物耐药性(AMR)基因。春季和夏季大肠杆菌的流行率高于冬季。观察到一个不同的血清型库,其中超过53%的分离株可能与作为潜在动物宿主的牛源有关。AMR分析显示,100%的分离株携带至少两个抗微生物耐药性基因。认识到大肠杆菌的多样性可能有助于指导新的农业用水规则《食品安全现代化法案》《生产安全规则》中提出的农业用水评估。
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引用次数: 0
Increasing Food Safety Preparedness of Small and Emerging Food Businesses with Targeted Food Safety Training 通过有针对性的食品安全培训提高小型和新兴食品企业的食品安全准备
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-031
A. Kinchla
There is strong consumer demand for local, value- added food products; however, these smaller scale food manufacturing operations tend to face more challenges in understanding and complying with food safety regulations, specifically, the Food Safety Modernization Act (FSMA) Preventive Controls for Human Food (PCHF) rule. An eight- module educational program was developed to introduce food safety throughout the product development life cycle and included concepts related to the FSMA PCHF rule. The program was offered in person (n = 2) and virtually (n = 6) to 143 participants in total, who were mostly early-stage food developers. Most (74%, n = 90) had little to no prior food safety-related training and experience. The majority (93%, n = 90) agreed they will apply the knowledge and skills learned in the course to food applications, such as developing food safety plans, label review, and good manufacturing practices. Self-rated level understanding for each module ranged from 4.4 to 4.6 on a 5-point Likert scale. Overall, participants felt better prepared to conduct various food safety activities after taking the course, supporting the need for non-mandatory training opportunities to enhance FSMA PCHF regulatory compliance. These types of training may be important for small-scale operations to improve the learning outcome and regulatory compliance.
消费者对本地增值食品有着强烈的需求;然而,这些规模较小的食品生产企业在理解和遵守食品安全法规,特别是《食品安全现代化法案》(FSMA)《人类食品预防控制》(PCHF)规则方面往往面临更多挑战。制定了一个八模块的教育计划,在整个产品开发生命周期中介绍食品安全,并包括与FSMA PCHF规则相关的概念。该项目由143名参与者亲自(n=2)和虚拟(n=6)参与,他们大多是早期食品开发人员。大多数人(74%,n=90)以前几乎没有或根本没有食品安全相关的培训和经验。大多数人(93%,n=90)同意将在课程中学到的知识和技能应用于食品应用,如制定食品安全计划、标签审查和良好生产实践。每个模块的自评水平理解在5分Likert量表上从4.4到4.6不等。总的来说,参与者在参加课程后感觉更好地准备好进行各种食品安全活动,这支持了非强制性培训机会的必要性,以提高FSMA PCHF的监管合规性。这些类型的培训对于小规模运营可能很重要,以提高学习成果和法规遵从性。
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引用次数: 0
Exploring Young Women’s Perceptions of Their Food Skills 探索年轻女性对自己饮食技巧的认知
Q4 Medicine Pub Date : 2023-04-01 DOI: 10.4315/fpt-22-032
J. Matthews
Many young adults mistakenly perceive that they have good food safety knowledge and are unlikely to experience foodborne illness. Young women’s food skills are of partic- ular importance because women are responsible for most food-related tasks in the home and many children learn food skills from their mothers. This descriptive qualitative study explored young women’s perceptions of food skills in three domains: food selection and planning, food prepara- tion, and food safety and storage. Through individual inter- views, 30 young women aged 17 to 30 years answered the following three key research questions: (i) What do food skills mean to you? (ii) How did you learn them? and (iii) In what areas are you most and least confident? Few participants mentioned food safety in their top-of- mind definition of food skills. More than half were least confident in the domain of food safety and storage. Fear prompted avoidance of cooking meat – even by those who were not vegan or vegetarian. Food skill interventions or curricula should emphasize food safety and storage so that young adults can reap the dietary and financial benefits of preparing all types of food. Consistent with others’ recom- mendations, the two most important food safety topics for educating young adults should be (i) cross-contamination and sanitation procedures and (ii) safe times and tempera- tures for cooking or storing food.
许多年轻人错误地认为他们有良好的食品安全知识,不太可能经历食源性疾病。年轻妇女的饮食技巧尤其重要,因为妇女在家里负责大多数与食物有关的工作,许多孩子从母亲那里学习饮食技巧。这项描述性定性研究探讨了年轻女性在三个领域对食品技能的看法:食品选择和规划,食品制备,食品安全和储存。通过个人访谈,30名年龄在17至30岁之间的年轻女性回答了以下三个关键研究问题:(i)食物技能对你意味着什么?(ii)你是怎么学会的?(3)你在哪些方面最自信,哪些方面最不自信?很少有参与者提到食品安全是他们对食品技能的首要定义。超过一半的人对食品安全和储存领域最缺乏信心。恐惧促使人们避免烹饪肉类——即使是那些不是素食主义者或素食主义者。食品技能干预措施或课程应强调食品安全和储存,以便年轻人能够从准备各种食品中获得饮食和经济上的好处。与其他人的建议一致,教育年轻人的两个最重要的食品安全主题应该是(i)交叉污染和卫生程序以及(ii)烹饪或储存食物的安全时间和温度。
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引用次数: 0
Risk Management Options to Reduce Human Salmonellosis Cases Due to Consumption of Raw Poultry 减少因食用生禽导致的人类沙门氏菌病病例的风险管理选择
Q4 Medicine Pub Date : 2023-03-01 DOI: 10.4315/fpt-22-035
A. Cohn
Salmonella cases due to cross-contamination by, or consumption of, raw poultry continue to be a major public health concern. Processors have yet to identify an effective “kill step” in raw poultry production, and food safety interventions may target many compartments of the supply chain, from breeder and grandparent flocks to consumer cooking practices, complicating the prioritization of specific areas to effectively manage risk. Moreover, raw poultry can be contaminated by diverse Salmonella serovars, ranging from multidrug resistant Salmonella Infantis to pansusceptible Salmonella Kentucky sequence type 152, which has substantially reduced likelihood of causing human disease. “Farm-to-table” risk models help assess the public health impact of different Salmonella risk management strategies and thereby inform policy priorities. This article provides an overview of risk management practices that should be considered and evaluated in Salmonella risk assessments, including risk management strategies focusing on (i) preharvest; (ii) slaughter and further processing; (iii) consumer product handling; and (iv) regulatory approaches. Data and model needs to allow assessment of these risk management strategies are also discussed. The information presented here represents a critical step in ensuring that future Salmonella risk assessment and risk management efforts represent a comprehensive systems approach and consider all potential options for Salmonella risk reduction.
由生家禽交叉污染或食用生家禽引起的沙门氏菌病例仍然是一个主要的公共卫生问题。加工商尚未确定生禽生产中有效的“屠宰步骤”,食品安全干预措施可能针对供应链的许多环节,从饲养员和祖辈鸡群到消费者的烹饪做法,这使确定特定领域的优先级以有效管理风险复杂化。此外,生家禽可能受到多种沙门氏菌血清型的污染,从耐多药的婴儿沙门氏菌到全敏感的肯塔基沙门氏菌序列152型,这大大降低了引起人类疾病的可能性。“从农场到餐桌”风险模型有助于评估不同沙门氏菌风险管理战略对公共卫生的影响,从而为政策重点提供信息。本文概述了沙门氏菌风险评估中应考虑和评估的风险管理实践,包括侧重于(1)收获前的风险管理策略;(ii)屠宰及进一步加工;(iii)消费品处理;(四)监管方法。本文还讨论了评估这些风险管理策略所需的数据和模型。这里提供的信息是确保未来沙门氏菌风险评估和风险管理工作的关键一步,代表了一种全面的系统方法,并考虑了减少沙门氏菌风险的所有潜在选择。
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引用次数: 0
Face Masks as Sources of Cross-Contamination during Food Preparation 口罩是食品制备过程中的交叉污染源
Q4 Medicine Pub Date : 2023-03-01 DOI: 10.4315/fpt-22-030
J. Acuff
Amid the COVID-19 pandemic, mask-wearing has become a common practice in the foodservice industry to prevent the spread of respiratory diseases. Like kitchen utensils, a mask may serve as a vehicle for cross-contamination of pathogens during food handling. The objective of this study was to quantify cross-contamination between tasks of handling contaminated chicken and chopping lettuce. Chicken breasts were inoculated with a high or a low level of nonpathogenic Escherichia coli surrogates (ca. 6 or 4 log CFU/ml) and sliced for 1, 5, or 10 min. During slicing, duplicate, single-use medical masks were touched each minute. One mask was immediately sampled, but the second mask was used to contaminate lettuce by touching the mask each minute while chopping the lettuce for 5 min. E. coli were enumerated from the second mask and lettuce. Masks touched while slicing both high- and low-inoculated chicken showed significant contamination (0.8–4.9 log CFU/cm2) after each slicing scenario of 1, 5, or 10 min (P > 0.05). Lettuce was significantly contaminated regardless of inoculation level (1.0–3.2 log CFU/g). Slicing time was a significant factor in some cases (P < 0.05), whereas inoculation level was not (P > 0.05). Data indicate masks can be a source of cross-contamination if not replaced appropriately.
在新冠肺炎大流行期间,戴口罩已成为餐饮业防止呼吸道疾病传播的常见做法。与厨房用具一样,口罩也可以作为食物处理过程中病原体交叉污染的载体。本研究的目的是量化处理受污染鸡肉和切生菜任务之间的交叉污染。用高或低水平的非致病性大肠杆菌替代物(约6或4 log CFU/ml)接种鸡胸肉,并切片1、5或10分钟。在切片过程中,每分钟接触重复的一次性医用口罩。一个口罩被立即取样,但第二个口罩被用来污染生菜,因为在切生菜5分钟时,每分钟都要触摸口罩。从第二个面罩和生菜中计数大肠杆菌。在每次切片1、5或10分钟后,切片高接种和低接种鸡肉时接触的口罩显示出显著污染(0.8–4.9 log CFU/cm2)(P>0.05)。无论接种水平如何,生菜都会受到显著污染(1.0–3.2 log CFU/g)。在某些情况下,切片时间是一个重要因素(P<0.05),而接种水平则不是(P>0.05)。数据表明,如果不适当更换口罩,口罩可能会成为交叉污染的来源。
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引用次数: 0
Food Safety Knowledge and Practices among Household Food Handlers in Mangweni Village of Mpumalanga Province in South Africa 南非普马兰加省Mangweni村家庭食品加工商的食品安全知识与实践
Q4 Medicine Pub Date : 2023-03-01 DOI: 10.4315/fpt-22-025
Tirhani Asnath Masia
The study aimed to assess food safety knowledge and practices among household food handlers in Mangweni village, South Africa. A quantitative cross-sectional study was conducted on 120 households selected using systematic random sampling. Women over 18 years of age who were responsible for preparing food in the selected households were eligible for inclusion in the study. A simple random sample was used to select 15 from the 120 food handlers to observe their food handling practices. SPSS version 27 was used to run the descriptive statistics. Food handlers were between 19 and 43 years of age, with a mean age of 29.9 ± 1.39. Most food handlers had high school (58.3%) or university or college (33.3%) educations. Levels of food safety knowledge were found to be poor (44.5%), good (53.9%), and excellent (1.6%). Our findings show the need for an intervention to improve food safety knowledge and practices among food handlers.
这项研究旨在评估南非Mangweni村家庭食品经营者的食品安全知识和做法。采用系统随机抽样对120户家庭进行了定量横断面研究。在选定的家庭中负责准备食物的18岁以上的妇女有资格参加这项研究。使用简单的随机样本从120名食品处理人员中选择15名,观察他们的食品处理实践。使用SPSS 27版进行描述性统计。食品管理员年龄在19至43岁之间,平均年龄为29.9±1.39岁。大多数食品管理员受过高中(58.3%)或大学或学院(33.3%)教育。食品安全知识水平分为差(44.5%)、好(53.9%)和优(1.6%)。我们的研究结果表明,有必要采取干预措施来提高食品经营者的食品安全知识和做法。
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引用次数: 0
Food Allergy Knowledge and Attitudes of Owners and Managers of Independently Owned Restaurants in Metro Orlando, Florida 佛罗里达州奥兰多市区独立餐厅老板和经理的食物过敏知识和态度
Q4 Medicine Pub Date : 2023-03-01 DOI: 10.4315/fpt-22-029
Anirudh Naig
Owners and managers (n = 102) of independently owned restaurants in Metro Orlando, FL, completed a web-based questionnaire that assessed food allergy knowledge and attitudes. Participants were confident in their knowledge of food allergens (4.28 ± 0.63). The attitude item with the lowest mean score was “not all restaurants needed to comply with food allergy policies” (2.70 ± 1.26), suggesting participants believed compliance with food allergy policies was critical in all restaurants. Eighty (78.4%) participants correctly answered all 10 knowledge questions. The mean knowledge score was 7.7 of 10 possible points. Florida requires foodservice workers to be knowledgeable about food allergies but does not mandate food allergy training or certification. It is in the best interest of foodservice establishments to train workers on food allergies to safeguard customer health.
佛罗里达州奥兰多市独立餐厅的老板和经理(n = 102)完成了一份基于网络的问卷,评估食物过敏知识和态度。参与者对食物过敏原的知识有信心(4.28±0.63)。平均得分最低的态度项是“并非所有餐馆都需要遵守食物过敏政策”(2.70±1.26),表明参与者认为遵守食物过敏政策对所有餐馆都至关重要。80名(78.4%)参与者正确回答了所有10个知识问题。平均知识得分为7.7分(满分10分)。佛罗里达州要求餐饮服务人员了解食物过敏,但不要求进行食物过敏培训或认证。对工作人员进行食物过敏方面的培训,以保障顾客的健康,符合餐饮服务机构的最大利益。
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引用次数: 0
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Food Protection Trends
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