Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.402
R. Lestari, Nour Athiroh Abdoes Sjakoer, N. Mubarakati
Hypertension is a chronic disease that is most often found in society. Hypertension is associated with increased production of superoxide radicals and endothelial dysfunction, which causes oxidative stress characterized by increased levels of MDA. Mango mistletoe contains secondary metabolic compounds such as flavonoids which act as natural antioxidants. The use of antioxidants from manga parasites is an alternative to neutralize the increase in MDA in the body and neutralize free radicals. This study aims to determine the levels of MDA in hypertensive rats (DOCA-Salt) exposed to Mango mistletoe Methanolic Extract (EMBM) for 14 days. This study used the true experimental design method with completely randomized design on 50 male Wistar rats, differentiated into 5 treatments, namely control (-), control (+), treatment 1 dose 50 mg / KgBB, treatment 2 doses of 100 mg / KgBB and treatment 3 doses 200 mg / KgBW. Each treatment had 5 replications. Data analysis used oneway ANOVA and post hoc tests, namely JAMOVI version 1.1.9.0 which was used to determine differences in pulmonary MDA levels between groups and pulmonarymalondialdehyde (MDA) were analyzed by spectrophotometry. The results of this study indicated that pulmonary MDA levels in hypertensive rats (Doca salt) exposed to EMBM showed very significant differences between all groups. This is evidenced by the p value analysis that is <0.05, namely <0.001 so that it can be concluded that EMBM can reduce MDA levels in rats Hypertension. This effect is controlled by EMBM at a dose of 50 mg / KgBB which is the optimum dose in reducing pulmonarymalondialdehyd. Keywords : Hypertension, Mango mistletoe, MDA ABSTRAK Hipertensi merupakan penyakit kronik yang paling kerap ditemukan di masyarakat. Hipertensi berkaitan dengan peningkatan produksi radikal superoksida dan disfungsi endotel, yang menyebabkan stress oksidatif ditandai dengan meningkatnya kadar MDA. Benalu mangga mengandung senyawa metabolic sekunder seperti flavanoid yang berperan sebagai antioksidan alami. Penggunaan antioksidan dari benalu mangga menjadi salah satu alternatif untuk menetralisir terjadinya peningkatan MDA dalam tubuh dan menetralkan radikal bebas. Penelitian ini bertujuan untuk mengetahui kadar MDA pada tikus hipertensi (DOCA-Garam) yang dipapar Ekstrak Metanolik Benalu Mangga (EMBM) selama 14 hari. Penelitian ini menggunakan metode true eksperimental design dengan Rancangan Acak Lengkap pada 50 ekor tikus wistar jantan, dibedakan menjadi 5 perlakuan yaitru control (-), control (+), perlakuan 1 dosis 50 mg/KgBB, perlakuan 2 dosis 100 mg/KgBB dan perlakuan 3 dosis 200 mg/KgBB. Setiap perlakuan terdapat 5 kali ulangan. Analisis data menggunakan uji ANOVA one way dan post hoc yaitu JAMOVI dengan versi 1.1.9.0 yang digunakan untuk mengetahui perbedaan kadar MDA paru antar kelompok dan pulmonarymalondialdehyde (MDA) dianalisis dengan spektrofotometri. Hasil penelitian ini menunjukan bahwa kadar MDA paru pada tikus hipertensi (Doca
高血压是一种常见于社会的慢性疾病。高血压与超氧自由基产生增加和内皮功能障碍有关,导致以MDA水平升高为特征的氧化应激。芒果槲寄生含有次生代谢化合物,如类黄酮,作为天然抗氧化剂。使用来自漫画寄生虫的抗氧化剂是一种替代方案,可以中和体内MDA的增加和中和自由基。本研究旨在测定暴露于芒果槲寄生甲醇提取物(EMBM) 14天的高血压大鼠(DOCA-Salt)的丙二醛水平。本研究采用完全随机设计的真实验设计方法,将50只雄性Wistar大鼠分为对照(-)、对照(+)5个处理,处理1剂量为50 mg / KgBW,处理2剂量为100 mg / KgBW,处理3剂量为200 mg / KgBW。每个处理有5个重复。数据分析采用单因素方差分析和事后检验,即JAMOVI版本1.1.9.0测定各组间肺丙二醛(MDA)水平的差异,分光光度法分析肺丙二醛(MDA)。本研究结果表明,EMBM处理的高血压大鼠(Doca盐)肺MDA水平在各组间存在非常显著的差异。p值分析结果<0.05,即<0.001,可以得出EMBM可以降低高血压大鼠MDA水平的结论。EMBM在50mg / KgBB的剂量下控制了这种效应,这是减少肺丙二醛的最佳剂量。关键词:高血压,芒果槲寄生,丙二醛Hipertensi berkaitan dengan peningkatan produksi radial superoksida dan disfungsi endotel, yang menyebabkan stress oksidatif ditandai dengan meningkatnya kadar MDA。黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物、黄酮类化合物。彭古那安反奥克西坦达里贝纳鲁曼加曼加迪萨拉萨萨萨萨替代性的untuk menetralisir terjadinya peningkatan MDA dalam tubuh dan menetralkan radialbebas。Penelitian ini bertujuan untuk mengetahui kadar MDA pada tikus hipertensi (doc - garam) yang dipapar Ekstrak Metanolik Benalu Mangga (EMBM) selama 14 hari。Penelitian ini menggunakan metode真正eksperimental设计dengan Rancangan Acak Lengkap 50篇ekor tikus纯种jantan, dibedakan menjadi 5 perlakuan yaitru控制(-)、控制(+),perlakuan 1剂量50毫克/ KgBB perlakuan 2剂量100毫克/ KgBB丹perlakuan 3剂量200毫克/ KgBB。设置perlakuan terdapat 5卡利乌兰干。分析数据为单因素方差分析(ANOVA),单因素方差分析(post - hoc yitu JAMOVI dengan vers1.1.9.0),单因素方差分析(dengan)。中文翻译:中文翻译:Hasil penelitian ini menunjukan bahwa kadar MDA paru pada tikus hipertensi (Doca- Garam) yang dipapar EMBM menunjukan perbedaan yang sangat nyata antara semua perkelompok。分析p值yitu < 0.05 yitu < 0.001 sehinga dapat dispulpulkan bahwa EMBM dapat menurunkan kadar MDA pagadtikus。Hipertensi。Efek ini dikendalikan oleh EMBM剂量50 mg/KgBB yang merupakan剂量最佳dalam menurunkan kadar MDA ParuKata Kunci: Hipertensi, Benalu Mangga, MDA
{"title":"Pengaruh Ekstrak Benalu Mangga Terhadap Kadar Malondealdehid (MDA) pada Tikus Hipertensi yang Dipapar DOCA- Garam","authors":"R. Lestari, Nour Athiroh Abdoes Sjakoer, N. Mubarakati","doi":"10.33474/e-jbst.v8i2.402","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.402","url":null,"abstract":"Hypertension is a chronic disease that is most often found in society. Hypertension is associated with increased production of superoxide radicals and endothelial dysfunction, which causes oxidative stress characterized by increased levels of MDA. Mango mistletoe contains secondary metabolic compounds such as flavonoids which act as natural antioxidants. The use of antioxidants from manga parasites is an alternative to neutralize the increase in MDA in the body and neutralize free radicals. This study aims to determine the levels of MDA in hypertensive rats (DOCA-Salt) exposed to Mango mistletoe Methanolic Extract (EMBM) for 14 days. This study used the true experimental design method with completely randomized design on 50 male Wistar rats, differentiated into 5 treatments, namely control (-), control (+), treatment 1 dose 50 mg / KgBB, treatment 2 doses of 100 mg / KgBB and treatment 3 doses 200 mg / KgBW. Each treatment had 5 replications. Data analysis used oneway ANOVA and post hoc tests, namely JAMOVI version 1.1.9.0 which was used to determine differences in pulmonary MDA levels between groups and pulmonarymalondialdehyde (MDA) were analyzed by spectrophotometry. The results of this study indicated that pulmonary MDA levels in hypertensive rats (Doca salt) exposed to EMBM showed very significant differences between all groups. This is evidenced by the p value analysis that is <0.05, namely <0.001 so that it can be concluded that EMBM can reduce MDA levels in rats Hypertension. This effect is controlled by EMBM at a dose of 50 mg / KgBB which is the optimum dose in reducing pulmonarymalondialdehyd. \u0000Keywords : Hypertension, Mango mistletoe, MDA \u0000ABSTRAK \u0000Hipertensi merupakan penyakit kronik yang paling kerap ditemukan di masyarakat. Hipertensi berkaitan dengan peningkatan produksi radikal superoksida dan disfungsi endotel, yang menyebabkan stress oksidatif ditandai dengan meningkatnya kadar MDA. Benalu mangga mengandung senyawa metabolic sekunder seperti flavanoid yang berperan sebagai antioksidan alami. Penggunaan antioksidan dari benalu mangga menjadi salah satu alternatif untuk menetralisir terjadinya peningkatan MDA dalam tubuh dan menetralkan radikal bebas. Penelitian ini bertujuan untuk mengetahui kadar MDA pada tikus hipertensi (DOCA-Garam) yang dipapar Ekstrak Metanolik Benalu Mangga (EMBM) selama 14 hari. Penelitian ini menggunakan metode true eksperimental design dengan Rancangan Acak Lengkap pada 50 ekor tikus wistar jantan, dibedakan menjadi 5 perlakuan yaitru control (-), control (+), perlakuan 1 dosis 50 mg/KgBB, perlakuan 2 dosis 100 mg/KgBB dan perlakuan 3 dosis 200 mg/KgBB. Setiap perlakuan terdapat 5 kali ulangan. Analisis data menggunakan uji ANOVA one way dan post hoc yaitu JAMOVI dengan versi 1.1.9.0 yang digunakan untuk mengetahui perbedaan kadar MDA paru antar kelompok dan pulmonarymalondialdehyde (MDA) dianalisis dengan spektrofotometri. Hasil penelitian ini menunjukan bahwa kadar MDA paru pada tikus hipertensi (Doca","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121090081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.515
Reza Anindita, Afrinia Eka Sari
One of the causes of the failure of anemia prevention therapy in pregnant women with the potential for stunting is the side effects of nausea and vomiting from Blood Supplement Tablets so most pregnant women choose not to consume iron supplements given by the government for free. This has opened up opportunities for the discovery of product innovations for probiotic drinks from jalembi juice (red guava, lemon, melon, beetroot) with a starter Lactobacillus plantarum to be developed as a candidate for a health drink to replace anti-anemia anemia for pregnant women. The purpose of this study was to test the total lactic acid bacteria (LAB), pH, and temperature of jalembi juice probiotic drink with L. plantarum starter. This research method was carried out by calculating the total amount of LAB of L. plantarum using the Total Plate Count (TPC), pH, and temperature of the jalembi juice probiotic drink. Data analysis was carried out using a quantitative descriptive approach. The results of this study indicated that the total LAB of L. plantarum contained in the jalembi juice probiotic drink was 1.7 x 108 CFU/ml with an average pH and temperature of 3.38 and 22.4 ºC respectively. This study concludes that the total LAB in the jalembi juice probiotic drink with L.plantarum starter according to the recommended standards can have an effect on body health, which is around 108 -109 CFU/ml.
{"title":"Uji Angka Lempeng Total (ALT) dan PH pada Produk Inovasi Minuman Probiotik Jalembi (Jambu Merah, Lemon, Melon, Bit) dengan Starter Lactobacillus plantarum","authors":"Reza Anindita, Afrinia Eka Sari","doi":"10.33474/e-jbst.v8i2.515","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.515","url":null,"abstract":"One of the causes of the failure of anemia prevention therapy in pregnant women with the potential for stunting is the side effects of nausea and vomiting from Blood Supplement Tablets so most pregnant women choose not to consume iron supplements given by the government for free. This has opened up opportunities for the discovery of product innovations for probiotic drinks from jalembi juice (red guava, lemon, melon, beetroot) with a starter Lactobacillus plantarum to be developed as a candidate for a health drink to replace anti-anemia anemia for pregnant women. The purpose of this study was to test the total lactic acid bacteria (LAB), pH, and temperature of jalembi juice probiotic drink with L. plantarum starter. This research method was carried out by calculating the total amount of LAB of L. plantarum using the Total Plate Count (TPC), pH, and temperature of the jalembi juice probiotic drink. Data analysis was carried out using a quantitative descriptive approach. The results of this study indicated that the total LAB of L. plantarum contained in the jalembi juice probiotic drink was 1.7 x 108 CFU/ml with an average pH and temperature of 3.38 and 22.4 ºC respectively. This study concludes that the total LAB in the jalembi juice probiotic drink with L.plantarum starter according to the recommended standards can have an effect on body health, which is around 108 -109 CFU/ml.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134916208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gram positive and negative bacteria have always been a problem for the survival of living things in a high threshold as normal flora. Telang flower contains anthocyanins which are efficacious as antioxidants and antibacterials so that kombucha has a high potential to be fermented by kombucha as the latest biotechnology product innovation in improving the immune system with the addition of a concentration of Baduy honey Product SR12 which has the potential to inhibit the growth of pathogens. This study aims to produce information regarding the concentration of Baduy honey product SR12 which has the potential as a gram-positive and gram-negative antibacterial. The research design used was a randomized block design which was divided into 2 factors. Factor I is the Concentration of Telang Flower Kombucha Sugar (Clitoria ternatea L) solution in the first fermentation (20%, 30%, and 40%) and factor II is the concentration of baduy honey product SR12, namely (20%, 30%, and 40%). Each treatment was repeated 3 times. The results obtained were processed using statistical analysis using one-way ANOVA at the 95% level. If the data from the research results have significant differences, ideally it can be followed up using a post hoc test. The results obtained in this study were the concentration of Baduy honey Product SR12 was positively correlated in preventing the growth of pathogens in both gram-positive and gram-negative bacteria. The conclusion in this study is that kombucha flower telang has the ability as a gram positive and negative antibacterial and can also be developed as the latest biotechnology product innovation. Telang flower kombucha fermentation with a concentration of Baduy honey Product SR12 of 40% had the highest antibacterial activity when compared to fermented telang flower kombucha at concentrations of Baduy honey Product SR12 with a concentration of 20% and 30%. The average diameter of the inhibition zone produced by Staphylococcus aureus bacteria is 20.08 mm in the strong category, Staphylococcus epidermidis 17.98 mm in the strong category, Pseudomonas aeruginosa 17.27 mm in the strong category, and Escherichia coli 16.59 mm strong category.
{"title":"Fermentasi Bunga Telang (Clitoria ternatea L) Dengan Penambahan Madu Baduy Produk SR12 Sebagai Inovasi Bioteknologi Kombucha","authors":"Mu’jijah Mu’jijah, Nurullah Asep Abdilah, Firman Rezaldi, Kusumiyati Kusumiyati, Diyan Yunanto Setyaji, M. Fadillah","doi":"10.33474/e-jbst.v8i2.496","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.496","url":null,"abstract":"Gram positive and negative bacteria have always been a problem for the survival of living things in a high threshold as normal flora. Telang flower contains anthocyanins which are efficacious as antioxidants and antibacterials so that kombucha has a high potential to be fermented by kombucha as the latest biotechnology product innovation in improving the immune system with the addition of a concentration of Baduy honey Product SR12 which has the potential to inhibit the growth of pathogens. This study aims to produce information regarding the concentration of Baduy honey product SR12 which has the potential as a gram-positive and gram-negative antibacterial. The research design used was a randomized block design which was divided into 2 factors. Factor I is the Concentration of Telang Flower Kombucha Sugar (Clitoria ternatea L) solution in the first fermentation (20%, 30%, and 40%) and factor II is the concentration of baduy honey product SR12, namely (20%, 30%, and 40%). Each treatment was repeated 3 times. The results obtained were processed using statistical analysis using one-way ANOVA at the 95% level. If the data from the research results have significant differences, ideally it can be followed up using a post hoc test. The results obtained in this study were the concentration of Baduy honey Product SR12 was positively correlated in preventing the growth of pathogens in both gram-positive and gram-negative bacteria. The conclusion in this study is that kombucha flower telang has the ability as a gram positive and negative antibacterial and can also be developed as the latest biotechnology product innovation. Telang flower kombucha fermentation with a concentration of Baduy honey Product SR12 of 40% had the highest antibacterial activity when compared to fermented telang flower kombucha at concentrations of Baduy honey Product SR12 with a concentration of 20% and 30%. The average diameter of the inhibition zone produced by Staphylococcus aureus bacteria is 20.08 mm in the strong category, Staphylococcus epidermidis 17.98 mm in the strong category, Pseudomonas aeruginosa 17.27 mm in the strong category, and Escherichia coli 16.59 mm strong category.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114954920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.497
L. Wulandari, Khotibul Umam
Kirinyuh (Chromolaena odorata) is one of the wild plants found that is difficult to eradicate because it has a high defense, so this plants is considered a weed plant that is detrimental to farmers and planters. However, the kirinyuh plant has the potential to be developed as an antibacterial. One of the natural ingredients that show pharmacological effects is flavonoids. This study aims to determine the antibacterial activity, and the effective concentration of kirinyuh extract in inhibiting the growth of Enterobacter sakazakii, Salmonellla typi, and Listeria monocytogenes bacteria. To get the extract used extraction method by maceration of immersion using 96% ethanol as solvent. The concentration of kirinyuh extract used was 20%, 40%, 60%, 80%, and 100%. While the positive control was used Ampicillin and negative control was used ethanol 96%, and for testing the antibacterial activity was carried out using the well difsusion method. The results of the antibacterial activity test were analyzed using ANOVA with a 95% confidance level and continued with Duncan’s Multiple Range Test (DMRT). The results showed that the extract concentrations of 20%, 40%, 60%, 80%, and 100% had given inhibitory activity to the growth of the test bacteria. At a concentration of 100%, kirinyuh extract was more effective as an antibacterial against bacteria Enterobacter sakazakii, Salmonella typi, and Listeria monocytogenes bacteria compared to other concentrations. The increase in the concentration of kirinyuh extract showed the larger the diameter of the inhibition zone for bacterial growth. The results of the inhibitory power test of kirinyuh extract against bacteria Enterobacter sakazakii with an average value of the inhibition zone of 6,66 mm, and against Salmonella typi the average value of the inhibition xone was 4,83 mm, and Listeria moncytogenes with an average value of 5, 60 mm.
Kirinyuh (Chromolaena odorata)是一种很难根除的野生植物,因为它有很高的防御能力,所以这种植物被认为是一种杂草植物,对农民和种植者有害。然而,麒麟柳植物具有开发抗菌药物的潜力。黄酮类化合物是一种具有药理作用的天然成分。本研究旨在测定麒龙提取物的抑菌活性,以及其抑制阪崎肠杆菌、型沙门氏菌和单核增生李斯特菌生长的有效浓度。以96%乙醇为溶剂,采用浸渍法提取。使用的麒麟柳提取物浓度分别为20%、40%、60%、80%和100%。以氨苄西林为阳性对照,96%乙醇为阴性对照,采用孔扩散法检测其抑菌活性。抗菌活性试验结果采用方差分析(ANOVA)进行分析,置信水平为95%,并继续采用Duncan 's Multiple Range test (DMRT)进行分析。结果表明,20%、40%、60%、80%和100%的提取物浓度对实验菌的生长均有抑制作用。当浓度为100%时,与其他浓度相比,麒麟柳提取物对阪崎肠杆菌、型沙门氏菌和单核增生李斯特菌的抑菌效果更好。随着麒麟柳提取物浓度的增加,细菌生长抑制带的直径越大。结果表明,基林柳提取物对阪崎肠杆菌的平均抑制带为6.66 mm,对型沙门氏菌的平均抑制带为4.83 mm,对单核细胞增生李斯特菌的平均抑制带为5.60 mm。
{"title":"Potensi Ekstrak Daun Kirinyuh (Chromolaena odorata) dalam Menghambat Bakteri Patogen (E. sakazakii, S. typi, dan L. monocytogenes)","authors":"L. Wulandari, Khotibul Umam","doi":"10.33474/e-jbst.v8i2.497","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.497","url":null,"abstract":"Kirinyuh (Chromolaena odorata) is one of the wild plants found that is difficult to eradicate because it has a high defense, so this plants is considered a weed plant that is detrimental to farmers and planters. However, the kirinyuh plant has the potential to be developed as an antibacterial. One of the natural ingredients that show pharmacological effects is flavonoids. This study aims to determine the antibacterial activity, and the effective concentration of kirinyuh extract in inhibiting the growth of Enterobacter sakazakii, Salmonellla typi, and Listeria monocytogenes bacteria. To get the extract used extraction method by maceration of immersion using 96% ethanol as solvent. The concentration of kirinyuh extract used was 20%, 40%, 60%, 80%, and 100%. While the positive control was used Ampicillin and negative control was used ethanol 96%, and for testing the antibacterial activity was carried out using the well difsusion method. The results of the antibacterial activity test were analyzed using ANOVA with a 95% confidance level and continued with Duncan’s Multiple Range Test (DMRT). The results showed that the extract concentrations of 20%, 40%, 60%, 80%, and 100% had given inhibitory activity to the growth of the test bacteria. At a concentration of 100%, kirinyuh extract was more effective as an antibacterial against bacteria Enterobacter sakazakii, Salmonella typi, and Listeria monocytogenes bacteria compared to other concentrations. The increase in the concentration of kirinyuh extract showed the larger the diameter of the inhibition zone for bacterial growth. The results of the inhibitory power test of kirinyuh extract against bacteria Enterobacter sakazakii with an average value of the inhibition zone of 6,66 mm, and against Salmonella typi the average value of the inhibition xone was 4,83 mm, and Listeria moncytogenes with an average value of 5, 60 mm.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126083220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.510
Blasius Atini, Yoseph Nahak Seran, Lusia Naimnule
Tubuhue Village is a village located in North Central Timor Regency with a large rice field area. This large rice field area was used as a research location to determine the level of distribution and the role of entomophagous insects as a biological control in the agroecosystem. The benefits of the value of entomophagous insects as a biological control for life encourage humans to maintain their sustainability and diversity. However, there is a possibility that the balance of entomophagous insects' ecosystem as a biological control in the area is disturbed because chemical pesticides damage the habitat they occupy. Knowing the diversity of entomophagous insects as biological controllers in rice fields is the aim of the study. Fafibola North Central Timor Regency and environmental aspects that affect it. This research took place in April – June 2022 in the Fafibola rice field area of North Central Timor Regency. The data collection technique in this study is insect nets. Data analysis used the Shannon Wiener Diversity Formula to measure entomophagous insects' types and levels of diversity. The diversity index of all species is in the medium category.
{"title":"Serangga Entomofagus Sebagai Biological Control di Areal Persawahan Fafibola Kabupaten Timor Tengah Utara","authors":"Blasius Atini, Yoseph Nahak Seran, Lusia Naimnule","doi":"10.33474/e-jbst.v8i2.510","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.510","url":null,"abstract":"Tubuhue Village is a village located in North Central Timor Regency with a large rice field area. This large rice field area was used as a research location to determine the level of distribution and the role of entomophagous insects as a biological control in the agroecosystem. The benefits of the value of entomophagous insects as a biological control for life encourage humans to maintain their sustainability and diversity. However, there is a possibility that the balance of entomophagous insects' ecosystem as a biological control in the area is disturbed because chemical pesticides damage the habitat they occupy. Knowing the diversity of entomophagous insects as biological controllers in rice fields is the aim of the study. Fafibola North Central Timor Regency and environmental aspects that affect it. This research took place in April – June 2022 in the Fafibola rice field area of North Central Timor Regency. The data collection technique in this study is insect nets. Data analysis used the Shannon Wiener Diversity Formula to measure entomophagous insects' types and levels of diversity. The diversity index of all species is in the medium category.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122534171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.521
S. Tridesianti
Poultry livestock are generally given feed in the form of corn kernels where the corn kernels contain high carbohydrates but have deficiencies of several amino acids. Amino acid deficiency can be overcome by increasing the protein content in the corn kernels. One way to increase the protein content in corn kernels is to carry out a fermentation process in corn flour solution. Therefore, this study aims to determine the effect of corn starch solution to increase dissolved protein levels in Saccharomyces cerevisiae biomass through a fermentation process. This study used a completely randomized design consisting of 8 treatments and 3 replications. The treatment volumes of corn flour solution were 0 mL, 5 mL, 10 mL, 15 mL, 20 mL, 25 mL, 30 mL and 35 mL. Data analysis used the calculation of variance and the BJND test. The results showed that the highest average dissolved protein content and total cell biomass of S. cerevisiae in the 15 ml treatment were 13.52%/g and 15.63 x 107 CFU/mL, the lowest average was in the 35 ml corn flour solution treatment, namely 0. 90%/gr and 3.01 x 107 CFU/mL. Based on the research, it can be concluded that corn flour solution can increase the dissolved protein content in S. cerevisiae biomass up to volume 15 mL/2 mL fermenter through the fermentation process. Based on the results of this study, it can be used as a basis for considering the use of S. cerevisiae fermenters to increase the nutritional value of corn seed-based animal feed.
{"title":"Peningkatan Kadar Protein Terlarut pada Larutan Tepung Jagung Menggunakan Saccharomyces cerevisiae","authors":"S. Tridesianti","doi":"10.33474/e-jbst.v8i2.521","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.521","url":null,"abstract":"Poultry livestock are generally given feed in the form of corn kernels where the corn kernels contain high carbohydrates but have deficiencies of several amino acids. Amino acid deficiency can be overcome by increasing the protein content in the corn kernels. One way to increase the protein content in corn kernels is to carry out a fermentation process in corn flour solution. Therefore, this study aims to determine the effect of corn starch solution to increase dissolved protein levels in Saccharomyces cerevisiae biomass through a fermentation process. This study used a completely randomized design consisting of 8 treatments and 3 replications. The treatment volumes of corn flour solution were 0 mL, 5 mL, 10 mL, 15 mL, 20 mL, 25 mL, 30 mL and 35 mL. Data analysis used the calculation of variance and the BJND test. The results showed that the highest average dissolved protein content and total cell biomass of S. cerevisiae in the 15 ml treatment were 13.52%/g and 15.63 x 107 CFU/mL, the lowest average was in the 35 ml corn flour solution treatment, namely 0. 90%/gr and 3.01 x 107 CFU/mL. Based on the research, it can be concluded that corn flour solution can increase the dissolved protein content in S. cerevisiae biomass up to volume 15 mL/2 mL fermenter through the fermentation process. Based on the results of this study, it can be used as a basis for considering the use of S. cerevisiae fermenters to increase the nutritional value of corn seed-based animal feed.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131178638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.312
Ahmad Taufiq Rohman, Ari Hayati, Ratna Djuniwati Lisminingsih
According to the republic of indonesia no undang-undang 18 years 2008, about waste management waste matter especially in kota-kota throughout the country right now this has been an issue that is always discussed the need to get the attention of all parties, no exception to the environment, poor islamic university campus how students perception about waste management 3r, based how about the model student perception of the trash can exist at community college islamic university, poor how are the awareness of student participation environmental quality to maintain a healthy and clean through waste management 3r, based this research use descriptive quantitative research methods and surveys in a field. directly A survey is data collection techniques specific techniques, than the other the percentage of perception student who answered a questionnaire in rata-rata show high marks, Student perception about waste management 3r based in college islamic university shows poor perception is very high obtained the results of ( 83-86 % ), a student knowing and perceiving a model that dumpster in the islamic university campus menunujuk very high percentage of poor showing the perception is very high obtained the results of ( 83-85 % ), the awareness of the students faculty mipa against participation menunujuk very high percentage of these things that indicated the result of ( 80-89 % )
根据印度尼西亚共和国no undang-undang 18年2008,关于废物管理废物问题,特别是在kota-kota全国各地,现在这一直是一个问题,需要得到各方的关注,不例外的环境,贫穷的伊斯兰大学校园,学生如何看待废物管理3r,基于模范学生如何看待垃圾可以存在于社区学院伊斯兰大学,学生参与环境质量的意识较差,如何通过废物管理来保持健康和清洁3r,本研究基于描述性定量研究方法和实地调查。直接A调查是数据收集技术的具体技术,比其他的感知学生谁回答了一份问卷的比例显示高分,学生对垃圾管理3r基于学院伊斯兰大学的感知显示差是非常高的,获得了结果(83- 86%)。一个学生知道和感知一个模式,即在伊斯兰大学校园垃圾桶菜单的百分比非常高,表现出的感知是非常高的,获得的结果(83- 85%),学生的意识,教师反对参与菜单的百分比非常高,这些事情表明的结果(80- 89%)。
{"title":"Persepsi Mahasiswa MIPA Terhadap Pengelolaan Sampah Padat Berbasis 3R Di Kampus Universitas Islam Malang","authors":"Ahmad Taufiq Rohman, Ari Hayati, Ratna Djuniwati Lisminingsih","doi":"10.33474/e-jbst.v8i2.312","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.312","url":null,"abstract":"According to the republic of indonesia no undang-undang 18 years 2008, about waste management waste matter especially in kota-kota throughout the country right now this has been an issue that is always discussed the need to get the attention of all parties, no exception to the environment, poor islamic university campus how students perception about waste management 3r, based how about the model student perception of the trash can exist at community college islamic university, poor how are the awareness of student participation environmental quality to maintain a healthy and clean through waste management 3r, based this research use descriptive quantitative research methods and surveys in a field. directly A survey is data collection techniques specific techniques, than the other the percentage of perception student who answered a questionnaire in rata-rata show high marks, Student perception about waste management 3r based in college islamic university shows poor perception is very high obtained the results of ( 83-86 % ), a student knowing and perceiving a model that dumpster in the islamic university campus menunujuk very high percentage of poor showing the perception is very high obtained the results of ( 83-85 % ), the awareness of the students faculty mipa against participation menunujuk very high percentage of these things that indicated the result of ( 80-89 % )","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"231 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132474615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.511
Nugroho Wibisono, Yoyon Arif Martino
Alstonia scholaris is a medicinal plant from the Apocynaceae family. Alstonia scholaris stem bark have some medicinal properties, but their effect on antidiabetics mechanisms is still unclear. Alstonia scholaris stem bark have some active compounds such as Echitamine, Beta Sitosterol, Lupeol, Alpha Amyrin Acetate, and Betulinic Acid. Present study was designed to find out the best active compounds of A. scholaris as a potential therapeutics agent against diabetes mellitus. This study involved the molecular docking of 3D structures of those active compounds into GLUT-1 receptor protein, visualized their result using Biovia Discovery Studio and pharmacokinetics profile prediction analysis using pkCSM web page. The molecular docking result show that Echitamine (-8,7 kcal/mol) have the lower energy compared to glibenclamide (-9,6 kcal/mol). Based on pkCSM web page, Echitamine were have a good pharmacokinetics profile.
{"title":"Uji Aktivitas Antidiabetes Kulit Batang Pulai (Alstonia scholaris) melalui Studi In Silico dan Prediksi Profil Farmakokinetika","authors":"Nugroho Wibisono, Yoyon Arif Martino","doi":"10.33474/e-jbst.v8i2.511","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.511","url":null,"abstract":"Alstonia scholaris is a medicinal plant from the Apocynaceae family. Alstonia scholaris stem bark have some medicinal properties, but their effect on antidiabetics mechanisms is still unclear. Alstonia scholaris stem bark have some active compounds such as Echitamine, Beta Sitosterol, Lupeol, Alpha Amyrin Acetate, and Betulinic Acid. Present study was designed to find out the best active compounds of A. scholaris as a potential therapeutics agent against diabetes mellitus. This study involved the molecular docking of 3D structures of those active compounds into GLUT-1 receptor protein, visualized their result using Biovia Discovery Studio and pharmacokinetics profile prediction analysis using pkCSM web page. The molecular docking result show that Echitamine (-8,7 kcal/mol) have the lower energy compared to glibenclamide (-9,6 kcal/mol). Based on pkCSM web page, Echitamine were have a good pharmacokinetics profile.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123007590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brick is a building material that is often used for residential walls. The bricks in this study were bricks with the addition of palm frond charcoal in the manufacturing process. The aims of this study were (1) to determine the effect of adding palm stem charcoal on the manufacture of red bricks in terms of compressive strength, water absorption (Suction rate) and apparent density (apparent density). (2) Comparing the test results with the SNI 15-2094-2000 standard. The manufacture of experimental bricks begins with adding clay with pulverized palm frond charcoal in a ratio of 100:0, 95:5, 90:10, 85:15 and 80:20, then molded, dried, burned and tested for new mechanical properties. brick. In this study, the compressive strength of bricks that enter class 100 is a ratio of 100:0 and 95:5 namely 11.75 Mpa and 10.10 Mpa, then bricks grade 50 is a ratio of 90:10 and 85:15 which is 8.08 Mpa and 6.27 Mpa. The water absorption capacity of bricks that meet the requirements of SNI 15-2094-2000 is a ratio of 100:0 and 95:5, namely 18.65% and 19.74%. The apparent density of bricks that meet the requirements of SNI 15-2094-2000 are the ratios of 100:0, 95:5, 90:10 and 85:15, which are 1.63 g/cm3, 1.54 g/cm3, 1,37 g/cm3 and 1.24 g/cm3. Bricks that meet the standards and are recommended as masonry bricks are bricks with a ratio of 95:5.
{"title":"Pengaruh Penambahan Arang Pelepah Sawit terhadap Sifat Mekanik Batu Bata Merah","authors":"Cory Dian Alfarisi, Padil Padil, Drastinawati Drastinawati, Wisrayetti Wisrayetti, Nurfatihayati Nurfatihayati, Yelmida A","doi":"10.33474/e-jbst.v8i2.498","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.498","url":null,"abstract":"Brick is a building material that is often used for residential walls. The bricks in this study were bricks with the addition of palm frond charcoal in the manufacturing process. The aims of this study were (1) to determine the effect of adding palm stem charcoal on the manufacture of red bricks in terms of compressive strength, water absorption (Suction rate) and apparent density (apparent density). (2) Comparing the test results with the SNI 15-2094-2000 standard. The manufacture of experimental bricks begins with adding clay with pulverized palm frond charcoal in a ratio of 100:0, 95:5, 90:10, 85:15 and 80:20, then molded, dried, burned and tested for new mechanical properties. brick. In this study, the compressive strength of bricks that enter class 100 is a ratio of 100:0 and 95:5 namely 11.75 Mpa and 10.10 Mpa, then bricks grade 50 is a ratio of 90:10 and 85:15 which is 8.08 Mpa and 6.27 Mpa. The water absorption capacity of bricks that meet the requirements of SNI 15-2094-2000 is a ratio of 100:0 and 95:5, namely 18.65% and 19.74%. The apparent density of bricks that meet the requirements of SNI 15-2094-2000 are the ratios of 100:0, 95:5, 90:10 and 85:15, which are 1.63 g/cm3, 1.54 g/cm3, 1,37 g/cm3 and 1.24 g/cm3. Bricks that meet the standards and are recommended as masonry bricks are bricks with a ratio of 95:5.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131215715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.33474/e-jbst.v8i2.408
F. Amalia, Hari Santoso, Hasan Zayadi
Javan langur population has decreased, because the natural habitat of Javan langurs is decreasing from year to year, in addition to the rampant fishing of langur for trading. In order to suppress and reduce the habitat destruction of these wild animals, an effective and sustainable land rehabilitation effort is needed by recording the types of forage plants that Javan langurs like and the behavior of Javan langurs in foraging for their food. One of the efforts made to create a natural breeding place. The purpose of this research is to determine whether there is a specific Javan langur eating behavior pattern, in addition to knowing whether there is a change in Javanese langur eating behavior towards the type of food given. This observation uses the Continuous Recording method of recording. The results obtained will be processed in a qualitative descriptive form (narrative). From the results of this study, it was found that there was a specific feeding behavior for each type of feed given based on the size of the leaves, when consuming calliandra with smaller leaves the langurs did not need to pick the leaves first, it was different when langurs ate mountain amethyst and telasih which were larger in size so they tended to picking and tearing it first to get smaller leaf size, and there is a change in the diet of the type of feed given, namely langurs tend to fight more for calliandra plants because calliandra has fruit as well as flowers and also smaller leaf size making it easier for langurs to eat it . This is also shown by the higher number of chews on calliandra, which is 25 chews for 10 seconds.
{"title":"Perilaku Makan Lutung Jawa (Trachypithecus auratus) di Pusat Rehabilitasi Lutung Jawa (JLC) Coban Talun Kota Batu","authors":"F. Amalia, Hari Santoso, Hasan Zayadi","doi":"10.33474/e-jbst.v8i2.408","DOIUrl":"https://doi.org/10.33474/e-jbst.v8i2.408","url":null,"abstract":"Javan langur population has decreased, because the natural habitat of Javan langurs is decreasing from year to year, in addition to the rampant fishing of langur for trading. In order to suppress and reduce the habitat destruction of these wild animals, an effective and sustainable land rehabilitation effort is needed by recording the types of forage plants that Javan langurs like and the behavior of Javan langurs in foraging for their food. One of the efforts made to create a natural breeding place. The purpose of this research is to determine whether there is a specific Javan langur eating behavior pattern, in addition to knowing whether there is a change in Javanese langur eating behavior towards the type of food given. This observation uses the Continuous Recording method of recording. The results obtained will be processed in a qualitative descriptive form (narrative). From the results of this study, it was found that there was a specific feeding behavior for each type of feed given based on the size of the leaves, when consuming calliandra with smaller leaves the langurs did not need to pick the leaves first, it was different when langurs ate mountain amethyst and telasih which were larger in size so they tended to picking and tearing it first to get smaller leaf size, and there is a change in the diet of the type of feed given, namely langurs tend to fight more for calliandra plants because calliandra has fruit as well as flowers and also smaller leaf size making it easier for langurs to eat it . This is also shown by the higher number of chews on calliandra, which is 25 chews for 10 seconds.","PeriodicalId":388660,"journal":{"name":"Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122948652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}