Iranian Journal of Pathology最新文献_第10页

Evaluation the -174G>C Genetic Polymorphism of Interleukin-6 in Iranian Patients with Chronic Lymphocytic Leukemia 伊朗慢性淋巴细胞白血病患者白细胞介素-6 -174G>C基因多态性的评价

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.544600.2790

Zakieh Rostamzadeh Khameneh, Mahshid Mohammadian, Ali Eishi Oskuie, Rahim Asghari, Mohadeseh Nemati

Background & Objective: Interleukin-6 (IL-6) is involved in inflammation and has a significant role in chronic lymphocytic leukemia (CLL) progression. Accordingly, IL-6 level may increase in CLL-affected patients compared to healthy individuals. The -174G>C single nucleotide polymorphism (SNP) in IL-6 promoter region has been related to differences in IL-6 transcription. Therefore, we investigated the possible association of IL-6 polymorphism with CLL.Methods: We examined the -174G>C SNP in IL-6 gene and studied its possible relationship with CLL in affected patients and in healthy controls using Amplification Refractory Mutation System- polymerase chain reaction genotyping method. IL-6 plasma level was measured in both studied groups.Results: According to the results, IL-6 mean plasma concentration was increased significantly in the CLL patients compared to the controls. However, 174G>C genotype of the IL-6 gene was not associated with CLL. Furthermore, there were no significant differences in the distribution of allele and genotype frequencies between the CLL-affected patients and the controls (P>0.05).Conclusion: Our study showed that -174G>C SNP in promotor of IL-6 gene could not be considered a risk factor for CLL. Larger prospective studies should be performed to confirm our results.

背景与目的:白细胞介素-6 (IL-6)参与炎症并在慢性淋巴细胞白血病(CLL)的进展中起重要作用。因此，与健康个体相比，cll患者的IL-6水平可能升高。IL-6启动子区-174G>C单核苷酸多态性(SNP)与IL-6转录差异有关。因此，我们研究了IL-6多态性与CLL的可能关联。方法:采用扩增难突变系统-聚合酶链式反应基因分型方法检测IL-6基因- 174g >C SNP，探讨其与慢性淋巴细胞白血病的关系。测定两组患者血浆IL-6水平。结果:结果显示，CLL患者IL-6平均血浆浓度较对照组明显升高。而IL-6基因的174G>C基因型与CLL无相关性。cll患者的等位基因分布及基因型频率与对照组比较差异无统计学意义(P>0.05)。结论:我们的研究表明IL-6基因启动子-174G>C SNP不能作为CLL的危险因素。需要进行更大规模的前瞻性研究来证实我们的结果。

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引用次数: 0

Maximum Surgical Blood Ordering Schedule in Common Pediatric Cardiac Surgeries in a Tertiary Center 三级医疗中心普通儿科心脏手术的最大手术订血时间表

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.545972.2804

Maryam Sotoudeh Anvari, Seyedeh Zohreh Hashemi, Mohammadreza Mirzaaghayan, Alireza Abdollahi, Mohammad Taghi Haghi Ashtiani, Abbas Akbari

Background & Objective: Unnecessary pre-operative ordering of red blood cells (RBCs) in elective surgeries increases costs and waste of blood inventory. Maximum surgical blood order schedule (MSBOS) is a helpful strategy in the estimation of blood units needed for surgery and the prevention of overconsumption. In this study, an MSBOS for pediatric cardiac surgeries is designed.Methods: In this cross-sectional study, we included all pediatric patients who underwent elective cardiac surgery in Children’s Medical Center in Tehran, Iran, from March 21, 2019, to September 22, 2019. Data consisted of the type of surgery and the number of blood units transfused and units cross-matched, based on which cross-match to transfusion ratio (CTR), the transfusion index (TI), and transfusion probability (T%) were calculated.Results: Overall 205 pediatric patients were included in the study. Four hundred and ten RBCs units were cross-matched, and 262 were transfused. The overall results of the CTR, T%, and TI for all the eight types of cardiac surgery were 1.56 (410/262), 76% (157/205), and 1.28 (262/205), respectively. The raw MSBOS for cardiac surgeries included ventricular septal defect, tetralogy of fallot, dextro-transposition of the great arteries, atrial septal defect, aortic coarctation, patent ductus arteriosus, pulmonary stenosis, and pacemaker insertion, which were 1.58, 1.03, 1.54, 1.66, 0.77, 0, 1.25, and 0 unit, respectively, and the figures were rounded up.Conclusion: Accurate MSBOS protocols reduce cross-match workload in laboratories, lead to the appropriate use of blood stocks with less wastage, save human and economic resources, and eventually, promote patient safety.

背景与目的:择期手术术前不必要的红细胞排序增加了成本和血液库存的浪费。最大手术血单计划(MSBOS)是一个有用的策略，在估计所需的血液单位手术和预防过度消耗。本研究设计了一套适用于小儿心脏手术的MSBOS。方法:在这项横断面研究中，我们纳入了2019年3月21日至2019年9月22日在伊朗德黑兰儿童医疗中心接受选择性心脏手术的所有儿科患者。数据包括手术类型、输血单位数和交叉配血单位数，根据交叉配血单位数计算输血比(CTR)、输血指数(TI)和输血概率(T%)。结果:共有205名儿科患者纳入研究。交叉配型410个红细胞，输262个。8种心脏手术的CTR、T%和TI的总体结果分别为1.56(410/262)、76%(157/205)和1.28(262/205)。心脏手术的原始MSBOS包括室间隔缺损、法洛四联症、大动脉右转、房间隔缺损、主动脉缩窄、动脉导管未闭、肺动脉狭窄和起搏器插入，分别为1.58、1.03、1.54、1.66、0.77、0、1.25和0个单位，数据进行四舍五入。结论:准确的MSBOS方案可减少实验室交叉配型工作量，合理使用血液储备，减少浪费，节省人力和经济资源，最终促进患者安全。

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引用次数: 0

Formalin Induced Micronucleus Formation in the Buccal Mucosa of Pathology Laboratory Workers 福尔马林诱导病理实验室工作人员口腔黏膜微核形成

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.1989457.3062

Ghazal Akhlaghi, Fatemeh Shahsavari, Maedeh Ghorbanpour

Background & Objective: Formaldehyde is an irritating substance that is categorized as a definite carcinogen (Group A1), according to the International Agency for Research on Cancer (IARC). This study was conducted to determine the role of this substance in the frequency of micronuclei (MN) in the buccal mucosal cells due to long-term exposure of the pathology staff to formaldehyde.Methods: In this case-control study, 32 pathology laboratory staff members were assigned to the case group, and 32 staff members who were not exposed to formaldehyde were assigned to the control group. Buccal mucosa cells were collected with a wet spatula and stained with Papanicolaou stain. In each sample, 500 cells were counted; then, the frequency of MN and the average number of MN in the micronucleated cells were assessed and compared between the 2 groups using the independent t test. Furthermore, the relationship between gender and MN was evaluated using the independent t test. The relationship between years of exposure and time of exposure during the day (in hours) for the case group, as well as the relationship between age and frequency of MN was analyzed using the Pearson correlation coefficient.Results: The mean frequency of MN in exfoliated buccal cells was 18.33±12.36 in the case group, which was significantly higher than the control group (10.55±6.22; P=0.003). The difference in the mean number of total MN in the micronucleated cells was not significant between the case and control groups (P=0.11). The relationship between sex, age, and years of exposure with the mean frequency of MN and the total number of MN in the micronucleated cells was not significant. The relationship between exposure time during the day and both the mean frequency of MN and the total number of MN in the micronucleated cells was significant (P=0.03). Conclusion: Formaldehyde exposure and extended time of exposure during the day can increase the frequency of MN, which can prognosticate the incidence of precancerous and cancerous lesions. Therefore, continuous exposure to formaldehyde can be considered an occupational health hazard, though further studies are needed to confirm this result.

背景与目的:根据国际癌症研究机构(IARC)的研究，甲醛是一种刺激性物质，被归类为明确的致癌物质(A1组)。本研究旨在确定长期暴露于甲醛的病理人员在口腔粘膜细胞中微核(MN)频率中的作用。方法:将32名病理实验室工作人员分为病例组，32名未接触甲醛的工作人员分为对照组。用湿抹刀收集口腔黏膜细胞，用巴氏染色法染色。在每个样本中，计数500个细胞;采用独立t检验，比较两组患者微核细胞中MN的出现频率和MN的平均数量。此外，使用独立t检验评估性别与MN之间的关系。使用Pearson相关系数分析病例组的暴露年数与白天暴露时间(以小时为单位)之间的关系，以及年龄与MN频率之间的关系。结果:病例组口腔脱落细胞MN的平均频率为18.33±12.36，显著高于对照组(10.55±6.22;P = 0.003)。微核细胞中MN的平均值与对照组比较差异无统计学意义(P=0.11)。性别、年龄和暴露年数与MN的平均频率和微核细胞中MN的总数之间的关系不显著。白天暴露时间与微核细胞中MN的平均频率和MN的总数有显著性关系(P=0.03)。结论:甲醛暴露及白天暴露时间延长可增加MN的发生频率，可预测癌前病变和癌性病变的发生。因此，持续接触甲醛可以被认为是一种职业健康危害，尽管需要进一步的研究来证实这一结果。

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引用次数: 0

Perianal Linear Syringocystadenoma Papilliferum: A Case Report with Review of the Literature 肛周线状淋巴囊腺瘤乳头状瘤1例并文献复习

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.1999659.3094

Naser Tayyebi Meibodi, Yalda Nahidi, Mostafa Izanlu, Negin Davoodi, Sima Davoodi

Syringocystadenoma papilliferum is a rare benign adnexal tumor that originates from the apocrine and eccrine glands. It mainly manifests as a solitary lesion in the head and neck; however, rarely, it may involve the trunk and limbs, typically with a linear pattern. Here, we report an extremely rare case of congenital linear syringocystadenoma papilliferum on the left buttock near the anus in a 6-year-old girl. This lesion should be considered in the list of differential diagnosis of linear lesions in order to prevent complications with proper diagnosis, treatment or follow-up.

摘要乳头状腺泡囊腺瘤是一种罕见的良性附件肿瘤，起源于大汗腺和汗腺。主要表现为头部和颈部的单发病变;然而，很少，它可能涉及躯干和四肢，典型的线性模式。在此，我们报告一例极为罕见的先天性线状脊髓囊腺瘤乳头状瘤，发生于一名六岁女孩的左臀部，靠近肛门。该病变应列入线状病变鉴别诊断清单中，以预防并发症的正确诊断、治疗或随访。

引用次数: 0

Serous Borderline Ovarian Epithelial Type Tumors of Testis in A Young Man with Only Mass Resection: A Case Report 年轻男性睾丸浆液性交界性卵巢上皮型肿瘤仅肿块切除一例报告

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.547886.2880

Mandana Rahimi, Yasaman Moradi, Farhood Khaleghimehr

Tumors of the ovarian epithelial type of testis are an infrequent entity. We report a case of borderline serous tumor in an 18-year-old male who presented with a right testicular mass, clinically suspicious of carcinoma. After right inguinal exploration, two pedunculated para-testicular masses were identified in the appendix of the right testis and epididymis. The histological features were as complex papillary structures lined by columnar cells with mild to moderate pleomorphism. Microscopically, features of borderline serous testicular tumors are identical to the morphology of the same tumors encountered in the ovarian counterparts. These tumors usually reveal papillae with fibrovascular cores lined by stratified cuboidal to columnar epithelium. This case highlights a need for clinicians and pathologists to be aware of this infrequent entity and improve the best patient management attitude. Serous epithelial tumors are common ovary tumors but are very rare entities in the testis. These tumors originate from the remnant of Mullerian ducts or Mullerian metaplasia of tunica vaginalis and are nonaggressive, even associated with extra ovarian spread, and have outstanding prognosis. A review of the literature has shown nearly fifty reported cases worldwide, and most of the cases occur in young to middle-aged adults.

卵巢上皮类型的睾丸肿瘤是一个罕见的实体。我们报告一例交界性浆液性肿瘤在一个18岁的男性谁提出了一个右睾丸肿块，临床怀疑癌。在右侧腹股沟探查后，在右侧睾丸和附睾的阑尾发现了两个带蒂的睾丸旁肿块。组织学特征为复杂的乳头状结构，排列着柱状细胞，有轻度至中度的多形性。显微镜下，交界性浆液性睾丸肿瘤的特征与卵巢肿瘤的形态相同。这些肿瘤通常显示有纤维血管核心的乳头状突起，内衬层状的立方到柱状上皮。这个病例强调了临床医生和病理学家需要意识到这种罕见的实体，并改善最佳的患者管理态度。浆液性上皮肿瘤是常见的卵巢肿瘤，但在睾丸是非常罕见的实体。这些肿瘤起源于阴道膜缪勒管的残余或缪勒管化生，无侵袭性，甚至与卵巢外扩散有关，预后良好。文献回顾显示，全世界有近50例报告病例，大多数病例发生在青年到中年人。

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引用次数: 0

Evaluation of INI1 Protein Expression Through IHC Study in Pediatric High-Grade Brain Tumors in South of Iran in 2008-2021 通过IHC研究评估伊朗南部2008-2021年儿童高级别脑肿瘤中INI1蛋白的表达

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-07-01 DOI: 10.30699/ijp.2023.561858.2973

Maryam Mohebbi, M. Shokripour, Maral Mokhtari

ABSTRACT

摘要

引用次数: 0

Simultaneous Genetically Detection of Streptococcus pyogenes, Streptococcus pneumoniae and Haemophilus influenzae in Patients with Treatment-Resistant Respiratory Infection 耐药呼吸道感染患者中化脓性链球菌、肺炎链球菌和流感嗜血杆菌的同时基因检测

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-07-01 DOI: 10.30699/ijp.2023.1991067.3075

Farzad Mohammadi Ebli, Z. Heshmatipour, Khadijeh Daneshjou, Seyed Davood Siadat

ABSTRACT

摘要

引用次数: 0

Virulence Determinants, Serotypes, and Antimicrobial Resistance of Rectovaginal Isolates of Streptococcus agalactiae. 无乳链球菌直肠阴道分离株的毒力决定因素、血清型和抗菌素耐药性。

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-01-01 DOI: 10.30699/IJP.2023.559137.2943

Shokoh Abotorabi, Zohreh Rasooli, Hamideh Pakniat, Fahimeh Baloo

Background & objective: This study was designed to determine the prevalence of serotypes, virulence-associated genes, and antimicrobial resistance of Streptococcus agalactiae in pregnant volunteers attending a major maternity hospital in Iran.

Methods: The virulence determinants and antimicrobial resistance profiles of 270 Group B streptococcus (GBS) samples were assessed in the adult participants. The prevalence of GBS serotypes, virulence-associated genes, and antimicrobial resistance of the isolates was determined.

Results: The GBS prevalence in the vaginal, rectal, and urinal carrier rates were 8.9%, 4.44%, and 4.44%, respectively, with no concomitant colonization. The serotypes Ia, Ib, and II were at a 1:2:1 ratio. The rectal isolates, harboring CylE, lmb, and bca genes, were of serotype Ia, susceptible to vancomycin. The serotype Ib from urine samples carrying three distinct virulence genes was susceptible to Ampicillin. In comparison, the same serotype with two virulence genes of CylE and lmb exhibited sensitivity to both Ampicillin and Ceftriaxone. The vaginal isolates belonged to serotype II with the CylE gene or serotype Ib with CylE and lmb genes. These isolates harboring the CylE gene were resistant to Cefotaxime. The overall antibiotic susceptibility range was 12.5-56.25%.

Conclusion: The findings broaden our understanding of the pathogenicity of the prevailing GBS colonization and predict different clinical outcomes.

背景与目的:本研究旨在确定在伊朗一家主要妇产医院就诊的孕妇志愿者中无乳链球菌的血清型、毒力相关基因和抗菌素耐药性的流行情况。方法:对270份成人B群链球菌(GBS)样本进行毒力决定因素和耐药性分析。测定了GBS血清型、毒力相关基因的流行情况以及分离株的抗微生物药物耐药性。结果:GBS在阴道、直肠和尿路的感染率分别为8.9%、4.44%和4.44%，未合并定植。血清型Ia、Ib和II的比例为1:2:1。直肠分离株携带cycle、lmb和bca基因，血清型为Ia型，对万古霉素敏感。携带三种不同毒力基因的尿样血清型Ib对氨苄青霉素敏感。相比之下，同一血清型具有两种毒力基因的CylE和lmb对氨苄西林和头孢曲松均敏感。阴道分离株为含CylE基因的血清II型或含CylE和lmb基因的血清Ib型。这些携带cycle基因的分离株对头孢噻肟具有耐药性。总体药敏范围为12.5 ~ 56.25%。结论:这些发现拓宽了我们对流行的GBS定植致病性的理解，并预测了不同的临床结果。

{"title":"Virulence Determinants, Serotypes, and Antimicrobial Resistance of Rectovaginal Isolates of Streptococcus agalactiae.","authors":"Shokoh Abotorabi, Zohreh Rasooli, Hamideh Pakniat, Fahimeh Baloo","doi":"10.30699/IJP.2023.559137.2943","DOIUrl":"https://doi.org/10.30699/IJP.2023.559137.2943","url":null,"abstract":"Background & objective: This study was designed to determine the prevalence of serotypes, virulence-associated genes, and antimicrobial resistance of Streptococcus agalactiae in pregnant volunteers attending a major maternity hospital in Iran.Methods: The virulence determinants and antimicrobial resistance profiles of 270 Group B streptococcus (GBS) samples were assessed in the adult participants. The prevalence of GBS serotypes, virulence-associated genes, and antimicrobial resistance of the isolates was determined.Results: The GBS prevalence in the vaginal, rectal, and urinal carrier rates were 8.9%, 4.44%, and 4.44%, respectively, with no concomitant colonization. The serotypes Ia, Ib, and II were at a 1:2:1 ratio. The rectal isolates, harboring CylE, lmb, and bca genes, were of serotype Ia, susceptible to vancomycin. The serotype Ib from urine samples carrying three distinct virulence genes was susceptible to Ampicillin. In comparison, the same serotype with two virulence genes of CylE and lmb exhibited sensitivity to both Ampicillin and Ceftriaxone. The vaginal isolates belonged to serotype II with the CylE gene or serotype Ib with CylE and lmb genes. These isolates harboring the CylE gene were resistant to Cefotaxime. The overall antibiotic susceptibility range was 12.5-56.25%.Conclusion: The findings broaden our understanding of the pathogenicity of the prevailing GBS colonization and predict different clinical outcomes.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"18 1","pages":"82-89"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9736724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Accuracy of Alpha-Methylacyl-CoA Racemase Immunohistochemical Expression for the Diagnosis of Ovarian and Endometrial Clear Cell Carcinomas. α -甲基酰基辅酶a消旋酶免疫组化表达诊断卵巢和子宫内膜透明细胞癌的准确性。

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-01-01 DOI: 10.30699/IJP.2023.556417.2925

Fatemeh Nili, Soheib Fathi, Mansoureh Tavakoli, Elham Mirzaian, Maryam Lotfi

Background & objective: Clear cell carcinoma (CCC) is an uncommon histopathologic subtype of ovarian and endometrial carcinoma. Due to the morphologic overlapping with other subtypes of ovarian and endometrial carcinomas, an accurate diagnosis is crucial.

Methods: In this study, 31 cases of ovarian clear cell carcinoma (OCCC), 28 endometrial clear cell carcinoma (ECCC), and 80 non-CCC subtypes (33 high-grade serous carcinomas of the ovary, 2 low-grade serous carcinomas, 10 ovarian endometrioid, 3 serous carcinomas and 29 endometrioid carcinomas of the endometrium) were investigated for immunohistochemical expression of AMACR. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for the distinction of OCCC and ECCC from other histopathologic subtypes were calculated.

Results: Positive AMACR staining was seen in 18 OCCCs (58%) and 10 ECCCs (35.7%). In the non-clear cell group, 44 cases of ovarian (98%) and 25 cases of endometrial carcinoma (78%) showed negative results. Only one case of ovarian endometrioid carcinoma and 7 cases (22%) of endometrial endometrioid carcinomas revealed a positive reaction (P<0.05). Collectively, sensitivity, specificity, PPV, and NPV of AMACR expression, for the diagnosis of OCCC were calculated as 58%, 98%, 94.7%, and 77.2%, respectively. The sensitivity, specificity, PPV, and NPV were shown to be as 35.7%, 78.1%, 58.8%, and 58.1%, respectively in the endometrium.

Conclusion: AMACR may be a highly specific immunohistochemical marker for the distinction of serous and clear cell carcinoma. A small percentage of endometrioid carcinoma may show positive staining. The sensitivity of this marker may not be higher than the other well-known Napsin-A IHC marker.

背景与目的:透明细胞癌(CCC)是卵巢和子宫内膜癌中一种罕见的组织病理学亚型。由于与其他亚型卵巢癌和子宫内膜癌的形态学重叠，准确诊断是至关重要的。方法:本研究对31例卵巢透明细胞癌(OCCC)、28例子宫内膜透明细胞癌(ECCC)和80例非ccc亚型(卵巢高级别浆液性癌33例、低级别浆液性癌2例、卵巢子宫内膜样癌10例、子宫内膜浆液性癌3例、子宫内膜样癌29例)进行AMACR免疫组化表达检测。计算OCCC和ECCC与其他组织病理学亚型区分的敏感性、特异性、阳性预测值(PPV)和阴性预测值(NPV)。结果:occc 18例(58%)，eccc 10例(35.7%)，AMACR染色阳性。非透明细胞组卵巢44例(98%)、子宫内膜癌25例(78%)阴性。仅1例卵巢子宫内膜样癌和7例子宫内膜样癌(22%)出现阳性反应。结论:AMACR可能是区分浆液性细胞癌和透明细胞癌的高度特异性免疫组织化学标志物。小部分子宫内膜样癌可呈阳性染色。该标记的敏感性可能不高于其他已知的Napsin-A IHC标记。

{"title":"Diagnostic Accuracy of Alpha-Methylacyl-CoA Racemase Immunohistochemical Expression for the Diagnosis of Ovarian and Endometrial Clear Cell Carcinomas.","authors":"Fatemeh Nili, Soheib Fathi, Mansoureh Tavakoli, Elham Mirzaian, Maryam Lotfi","doi":"10.30699/IJP.2023.556417.2925","DOIUrl":"https://doi.org/10.30699/IJP.2023.556417.2925","url":null,"abstract":"Background & objective: Clear cell carcinoma (CCC) is an uncommon histopathologic subtype of ovarian and endometrial carcinoma. Due to the morphologic overlapping with other subtypes of ovarian and endometrial carcinomas, an accurate diagnosis is crucial.Methods: In this study, 31 cases of ovarian clear cell carcinoma (OCCC), 28 endometrial clear cell carcinoma (ECCC), and 80 non-CCC subtypes (33 high-grade serous carcinomas of the ovary, 2 low-grade serous carcinomas, 10 ovarian endometrioid, 3 serous carcinomas and 29 endometrioid carcinomas of the endometrium) were investigated for immunohistochemical expression of AMACR. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for the distinction of OCCC and ECCC from other histopathologic subtypes were calculated.Results: Positive AMACR staining was seen in 18 OCCCs (58%) and 10 ECCCs (35.7%). In the non-clear cell group, 44 cases of ovarian (98%) and 25 cases of endometrial carcinoma (78%) showed negative results. Only one case of ovarian endometrioid carcinoma and 7 cases (22%) of endometrial endometrioid carcinomas revealed a positive reaction (P<0.05). Collectively, sensitivity, specificity, PPV, and NPV of AMACR expression, for the diagnosis of OCCC were calculated as 58%, 98%, 94.7%, and 77.2%, respectively. The sensitivity, specificity, PPV, and NPV were shown to be as 35.7%, 78.1%, 58.8%, and 58.1%, respectively in the endometrium.Conclusion: AMACR may be a highly specific immunohistochemical marker for the distinction of serous and clear cell carcinoma. A small percentage of endometrioid carcinoma may show positive staining. The sensitivity of this marker may not be higher than the other well-known Napsin-A IHC marker.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"18 1","pages":"57-63"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Programmed Death - Ligand 1 (Pd-L1) Expression in Head and Neck Squamous Cell Carcinoma. 程序性死亡配体1 (Pd-L1)在头颈部鳞状细胞癌中的表达

Q3 Medicine

Iranian Journal of Pathology

Pub Date : 2023-01-01 DOI: 10.30699/ijp.2023.1988955.3057

Aarya Unnikrishnan, Vijaya Basavaraj

Background & objective: Head and Neck Squamous Cell Carcinoma (HNSCC) is a highly frequent malignancy worldwide and is also the leading cause of death. The prognosis for individuals with HNSCC remains dismal, with a five-year survival rate of less than 50%. The novel anti-PD-L1 immunotherapy is found to be promising, and immunohistochemistry (IHC) has been established as a reliable method for patient stratification. We intend to evaluate the prognostic significance of the expression of programmed death ligand-1 (PD-L1) in HNSCC and determine its association with clinicopathological variables.

Methods: A total of 50 cases of biopsy-confirmed HNSCC were studied in a tertiary hospital between Dec 2020 and June 2022. The specimens were tested for PD-L1 IHC expression with antibody clone CAL-10 (Biocare) and scored by Combined Positive Score (CPS). The association between PD-L1 expression and clinicopathological variables was evaluated.

Results: PD-L1 was positive in 92% of the cases, and a significant association (P= 0.024) was seen between PD-L1 expression and tumor-infiltrating lymphocytes (TILs). PD-L1 did not show any significant association with patient demographics, tumor site, grade, or stage.

Conclusion: In the present study, evaluation of the immunohistochemical expression of PD-L1 on the tumor cells and TILs in HNSCC revealed a high prevalence of PD-L1 expression. PD-L1 IHC studies for patient selection for immunotherapy would be a promising technique. Frequent PD-L1 expression in tumors with significant TILs may be useful in identifying patients who may benefit from anti-PD-1/PD-L1 therapy.

背景与目的:头颈部鳞状细胞癌(HNSCC)是世界范围内常见的恶性肿瘤，也是导致死亡的主要原因。HNSCC患者的预后仍然很差，5年生存率不到50%。新的抗pd - l1免疫疗法被发现是有希望的，免疫组织化学(IHC)已被建立为一种可靠的患者分层方法。我们打算评估程序性死亡配体-1 (PD-L1)在HNSCC中表达的预后意义，并确定其与临床病理变量的关系。方法:对某三级医院2020年12月至2022年6月活检证实的HNSCC患者50例进行研究。采用抗体克隆CAL-10 (Biocare)检测PD-L1 IHC表达，并采用联合阳性评分(CPS)进行评分。评估PD-L1表达与临床病理变量之间的关系。结果:92%的病例PD-L1阳性，PD-L1表达与肿瘤浸润淋巴细胞(TILs)有显著相关性(P= 0.024)。PD-L1与患者人口统计学、肿瘤部位、分级或分期无显著相关性。结论:本研究通过对HNSCC肿瘤细胞和til中PD-L1的免疫组化表达的评价，发现PD-L1在HNSCC中具有较高的表达率。PD-L1免疫组化研究将是一项有前途的技术。在具有显著TILs的肿瘤中频繁的PD-L1表达可能有助于识别可能从抗pd -1/PD-L1治疗中获益的患者。

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引用次数: 0