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Histopathological Evaluation of PYGO2 Expression in Esophageal Squamous Cell Carcinoma.
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-10-02 DOI: 10.30699/ijp.2024.2024609.3269
Sima Ardalan Khales, Habibeh Rahmani Kalat, Sedigheh Soleymani, Amir Hossein Jafarian, Mohammad Mahdi Forghanifard

Background & objective: Esophageal squamous cell carcinoma (ESCC) is one of the world's deadliest cancer diseases. Deregulation of developmental signaling pathways such as Wnt/β-catenin is frequently implicated in a wide range of human cancers. The present study was designed to analyze the expression of the Pygopus2 (PYGO2) protein, the main co-activator of the Wnt/β-catenin signaling pathway, in ESCC tissues and evaluate its probable correlation with clinicopathological features of patients.

Methods: In this study, PYGO2 protein expression was assessed in tumors and margin normal tissues from 50 ESCC patients using immunohistochemical analysis, and its clinicopathological relevance in the patients was evaluated.

Results: A significant PYGO2 overexpression was observed in %32 of the tumor cells. Interestingly, PYGO2 expression was significantly correlated with the depth of tumor invasion (P= 0.021).

Conclusion: PYGO2 protein may be highly expressed in ESCC in correlation with the invasiveness of the disease. Therefore, it may be used as a biomarker for diagnosis of invasive ESCC and a putative therapeutic target to inhibit ESCC invasiveness.

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引用次数: 0
Immunohistochemical Expression of Programmed Death Ligand- 1 (PD-L1) in Colorectal Carcinoma; A Cross-sectional Study. 程序性死亡配体 1 (PD-L1) 在结直肠癌中的免疫组化表达;一项横断面研究。
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2023-12-29 DOI: 10.30699/IJP.2023.1988660.3054
Shruti Tadachina, Sheela Devi Shivalingaiah, Mahesh Shetty

Background & objective: Colorectal carcinoma (CRC) is one of the most common cancers worldwide. The interaction of programmed cell death receptor 1 (PD-1) and programmed death ligand 1 (PD-L1) plays an important role by inhibiting the immune mechanism by which cancer cells escape antitumor immunity. Immunotherapy using checkpoint inhibitors is a growing treatment modality in many cancers; one such is anti-PD1/PD-L1. The present study aimed to study the immunohistochemical (IHC) expression of PD-L1 in CRC and its association with various known clinicopathological parameters.

Methods: This study was a 2-year prospective study and included 34 colectomy specimens diagnosed as colorectal adenocarcinoma. The expression of PD-L1 was evaluated on tumoral cells and tumor-infiltrating immune cells (TIICs) and was correlated with various clinicopathological parameters.

Results: Immunohistochemical expression of PD-L1 on tumoral cells and tumor microenvironment in CRC revealed positivity in 17.65% of cases each. The PD-L1 expression on tumoral cells was associated with lymphovascular invasion (LVI) and perineural invasion (PNI) with P- values of 0.012 and 0.005, respectively, while PD-L1 expression on TIICs was associated with tumor budding with a P-value of 0.022.

Conclusion: IHC expression of PD-L1 on tumoral cells and immune cells may be associated with some known poor prognostic factors. Since anti-PD1/PD-L1 is used for targeted therapy, it may be beneficial and economically feasible to evaluate PD-L1 in CRC and establish its role as a prognostic factor.

背景与目的:结直肠癌(CRC)是全球最常见的癌症之一。程序性细胞死亡受体 1(PD-1)和程序性死亡配体 1(PD-L1)之间的相互作用通过抑制免疫机制发挥着重要作用,癌细胞通过这种机制逃避抗肿瘤免疫。使用检查点抑制剂的免疫疗法是许多癌症中一种不断发展的治疗方式,抗PD1/PD-L1就是其中之一。本研究旨在研究 PD-L1 在 CRC 中的免疫组化(IHC)表达及其与各种已知临床病理参数的关系:本研究是一项为期两年的前瞻性研究,纳入了 34 例被诊断为结直肠腺癌的结肠切除标本。评估了肿瘤细胞和肿瘤浸润免疫细胞(TIICs)中 PD-L1 的表达,并将其与各种临床病理参数进行了相关分析:结果:PD-L1在CRC肿瘤细胞和肿瘤微环境中的免疫组化表达显示阳性的病例各占17.65%。肿瘤细胞上的 PD-L1 表达与淋巴管侵犯(LVI)和神经周围侵犯(PNI)相关,P 值分别为 0.012 和 0.005,而 TIICs 上的 PD-L1 表达与肿瘤出芽相关,P 值为 0.022:结论:PD-L1在肿瘤细胞和免疫细胞上的IHC表达可能与一些已知的不良预后因素有关。由于抗 PD1/PD-L1 可用于靶向治疗,因此对 CRC 中的 PD-L1 进行评估并确定其作为预后因素的作用可能是有益且经济可行的。
{"title":"Immunohistochemical Expression of Programmed Death Ligand- 1 (PD-L1) in Colorectal Carcinoma; A Cross-sectional Study.","authors":"Shruti Tadachina, Sheela Devi Shivalingaiah, Mahesh Shetty","doi":"10.30699/IJP.2023.1988660.3054","DOIUrl":"10.30699/IJP.2023.1988660.3054","url":null,"abstract":"<p><strong>Background & objective: </strong>Colorectal carcinoma (CRC) is one of the most common cancers worldwide. The interaction of programmed cell death receptor 1 (PD-1) and programmed death ligand 1 (PD-L1) plays an important role by inhibiting the immune mechanism by which cancer cells escape antitumor immunity. Immunotherapy using checkpoint inhibitors is a growing treatment modality in many cancers; one such is anti-PD1/PD-L1. The present study aimed to study the immunohistochemical (IHC) expression of PD-L1 in CRC and its association with various known clinicopathological parameters.</p><p><strong>Methods: </strong>This study was a 2-year prospective study and included 34 colectomy specimens diagnosed as colorectal adenocarcinoma. The expression of PD-L1 was evaluated on tumoral cells and tumor-infiltrating immune cells (TIICs) and was correlated with various clinicopathological parameters.</p><p><strong>Results: </strong>Immunohistochemical expression of PD-L1 on tumoral cells and tumor microenvironment in CRC revealed positivity in 17.65% of cases each. The PD-L1 expression on tumoral cells was associated with lymphovascular invasion (LVI) and perineural invasion (PNI) with P- values of 0.012 and 0.005, respectively, while PD-L1 expression on TIICs was associated with tumor budding with a P-value of 0.022.</p><p><strong>Conclusion: </strong>IHC expression of PD-L1 on tumoral cells and immune cells may be associated with some known poor prognostic factors. Since anti-PD1/PD-L1 is used for targeted therapy, it may be beneficial and economically feasible to evaluate PD-L1 in CRC and establish its role as a prognostic factor.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 1","pages":"22-30"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141307059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation of Tumor Vascular Microenvironment by Nestin and Fms-related Tyrosine Kinase 1 (FLT1) and Their Prognostic Significance in Renal Cell Carcinoma. Nestin和fms相关酪氨酸激酶1 (FLT1)对肾细胞癌肿瘤血管微环境的调节及其预后意义
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-03-03 DOI: 10.30699/IJP.2024.2024190.3266
Noha Elkady, Reham Ahmed Abdelaziz, Rasha Adel Abdelmoneum, Ahmed S Ghonaimy, Dina Mohamed Allam

Background & objective: Hypervascularity is a characteristic feature of renal cell carcinoma (RCC) and microvessel density (MVD) predicts tumor metastasis. Nestin is a stem cell marker that is expressed in proliferating endothelial cells and newly formed vessels and Fms-related tyrosine kinase 1 (FLT1) is a proangiogenic factor. This study aimed to evaluate the expression of Nestin and FLT1 in RCC and their prognostic impact.

Methods: This retrospective study included sixty cases of RCC after obtaining ethical approval. Sections were immunohistochemically stained by Nestin and FLT1 then their expressions were compared to different clinicopathological parameters. MVD was evaluated using Nestin and CD34 and compared to the different parameters.

Results: Nestin was expressed mainly in endothelial cells of small vessels in 65% of cases while FLT1 was expressed in tumor and endothelial cells in 73.3% of cases. Their expressions were significantly associated with aggressive tumor parameters including larger tumors, high-grade tumors, wider tumor extension, and advanced stage. Moreover, Nestin expression was significantly associated with metastasis. MVD evaluated by Nestin showed more associations with larger tumors, high-grade tumors, wider tumor extension, advanced stage, and metastasis than MVD measured by CD34. Nestin and FLT1 positivity and high MVD measured by Nestin were significantly associated with short overall survival.

Conclusion: Nestin and FLT1 expressions in RCC may be associated with aggressive tumor features and short patients' overall survival. MVD evaluated by Nestin may be correlated with tumor progression and metastasis. Nestin and FLT1 may be used as prognostic biomarkers in RCC.

背景与目的:血管过多是肾细胞癌(RCC)的一个特征,微血管密度(MVD)可预测肿瘤转移。Nestin是一种干细胞标记物,可在增殖的内皮细胞和新形成的血管中表达,而Fms相关酪氨酸激酶1(FLT1)是一种促血管生成因子。本研究旨在评估 Nestin 和 FLT1 在 RCC 中的表达及其对预后的影响:这项回顾性研究在获得伦理批准后纳入了 60 例 RCC 病例。切片经 Nestin 和 FLT1 免疫组化染色后,将其表达与不同的临床病理参数进行比较。用Nestin和CD34评估MVD,并与不同的参数进行比较:结果:65%的病例中,Nestin主要在小血管内皮细胞中表达,而73.3%的病例中,FLT1在肿瘤和内皮细胞中表达。它们的表达与侵袭性肿瘤参数(包括肿瘤较大、高级别肿瘤、肿瘤扩展范围较广和晚期)明显相关。此外,Nestin的表达与肿瘤转移也有明显相关性。与用CD34测量的MVD相比,用Nestin评估的MVD与更大的肿瘤、高级别肿瘤、更宽的肿瘤扩展范围、晚期分期和转移有更多的关联。Nestin和FLT1阳性以及Nestin测量的高MVD与总生存期缩短显著相关:结论:Nestin和FLT1在RCC中的表达可能与侵袭性肿瘤特征和患者较短的总生存期有关。结论:Nestin和FLT1在RCC中的表达可能与侵袭性肿瘤特征和患者总生存期缩短有关。Nestin和FLT1可作为RCC的预后生物标志物。
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引用次数: 0
Role of CK7 Immunoreactivity in Predicting Progression of the Cervical Intraepithelial Neoplasia: A Retrospective Cross-sectional Study.
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-10-02 DOI: 10.30699/IJP.2024.2019662.3239
Soheila Sarmadi, Fatemeh Nili, Kiana Anousha

Background & objective: Cervical intraepithelial squamous lesions (SIL) are the first step toward developing most cervical cancers. Moreover, most of the previous studies focusing on a reliable predictive factor of cervical cancer progression have been reported as inconclusive. Recently, immunophenotyping of squamocolumnar junction cells with CK7 has been introduced as a novel SIL detection and risk evaluation method. Therefore, we aimed to investigate the value of CK7 positivity and its pattern in predicting the progression of LSIL to high-grade counterparts.

Methods: This retrospective study was conducted in Yas Complex Hospital from 2016 to 2018 among patients of the clinic of colonoscopy. Patients with low-grade lesions were included in one group, and patients with high-grade lesions were considered as a control group. The immunoreactivity of CK7 by immunohistochemical staining pattern was interpreted in both groups.

Results: CK7 immunoreactivity was negative in 29.5% of the precancerous lesions and positive in 70.5%. The relationship between grade and CK7 staining was significant (P = 0.040). A significant relationship between the progression status of the disease and CK7 staining was obtained (P=0.001). CK7 staining showed a sensitivity of 82.35%, specificity of 81.82%, positive predictive value of 87.5%, negative predictive value of 75%, and diagnostic accuracy of 82.14% in predicting the progression of the SILs.

Conclusion: Our data and previous studies support the correlation of CK7 expression with a higher chance of progression of low-grade intraepithelial lesions.

{"title":"Role of CK7 Immunoreactivity in Predicting Progression of the Cervical Intraepithelial Neoplasia: A Retrospective Cross-sectional Study.","authors":"Soheila Sarmadi, Fatemeh Nili, Kiana Anousha","doi":"10.30699/IJP.2024.2019662.3239","DOIUrl":"10.30699/IJP.2024.2019662.3239","url":null,"abstract":"<p><strong>Background & objective: </strong>Cervical intraepithelial squamous lesions (SIL) are the first step toward developing most cervical cancers. Moreover, most of the previous studies focusing on a reliable predictive factor of cervical cancer progression have been reported as inconclusive. Recently, immunophenotyping of squamocolumnar junction cells with CK7 has been introduced as a novel SIL detection and risk evaluation method. Therefore, we aimed to investigate the value of CK7 positivity and its pattern in predicting the progression of LSIL to high-grade counterparts.</p><p><strong>Methods: </strong>This retrospective study was conducted in Yas Complex Hospital from 2016 to 2018 among patients of the clinic of colonoscopy. Patients with low-grade lesions were included in one group, and patients with high-grade lesions were considered as a control group. The immunoreactivity of CK7 by immunohistochemical staining pattern was interpreted in both groups.</p><p><strong>Results: </strong>CK7 immunoreactivity was negative in 29.5% of the precancerous lesions and positive in 70.5%. The relationship between grade and CK7 staining was significant (<i>P</i> = 0.040). A significant relationship between the progression status of the disease and CK7 staining was obtained (<i>P</i>=0.001). CK7 staining showed a sensitivity of 82.35%, specificity of 81.82%, positive predictive value of 87.5%, negative predictive value of 75%, and diagnostic accuracy of 82.14% in predicting the progression of the SILs.</p><p><strong>Conclusion: </strong>Our data and previous studies support the correlation of CK7 expression with a higher chance of progression of low-grade intraepithelial lesions.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 4","pages":"384-391"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FABP4, GINS2 and CBX7 Expression in Cancer Cervix Tissues: Clinical, Pathological and Prognostic Implications. 宫颈癌组织中 FABP4、GINS2 和 CBX7 的表达:临床、病理和预后意义
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2023-12-29 DOI: 10.30699/IJP.2023.1971325.2944
Mariem A Elfeky, Rema H Faraj Saad, Mohamed Ali Alabiad, Mohammed Alorini, Rehab Hemeda, Ramadan M Ali, Loay M Gertallah, Mohamed Negm, Ahmed Mahmoud Abdou, Ahmed Baker A Alshaikh, Ahmed Elmaasrawy

Background & objective: Cervical cancer spreads to the pelvic lymph nodes, leading to a high incidence of cancer recurrence and unfavorable survival rates. Therefore, there is an urgent need to detect new predictive biomarkers for the early assessment of pelvic lymph node status in patients with cervical cancer. The current study aimed to assess the expression of FABP4, GINS2, and CBX7 in cervical cancer tissue to detect their prognostic and predictive roles in developing lymph node metastases in patients with that cancer type.

Methods: We collected the tissues from patients with cervical cancer and evaluated the expression of FABP4, GINS2, and CBX7 using immunohistochemistry. We evaluated the association between their expression and clinicopathological and prognostic parameters.

Results: A high expression of FABP4 and GINS2 and a low expression of CBX7 were found to be positively associated with the old age group, large tumor size, high grade and lymphovascular involvement, para-uterine organ infiltration, advanced FIGO stage, chemotherapeutic resistance, and tumor recurrence.

Conclusion: We demonstrated the oncogenic roles of FABP4 and GISN2 in addition to the on-co-suppressive roles of CBX7 in cervical cancer and their association with poor clinicopathological criteria and poor survival. Our results may indicate that FABP4, GISN2, and CBX7 could be considered predictive biomarkers of the occurrence of lymph node metastases in the cancer of the cervix preoperatively, which could be beneficial in the accurate preoperative design therapy.

背景与目的:宫颈癌会扩散到盆腔淋巴结,导致癌症复发率高、生存率低。因此,迫切需要检测新的预测性生物标志物,用于早期评估宫颈癌患者盆腔淋巴结的状态。本研究旨在评估 FABP4、GINS2 和 CBX7 在宫颈癌组织中的表达情况,以检测它们在宫颈癌患者发生淋巴结转移时的预后和预测作用:我们收集了宫颈癌患者的组织,并使用免疫组化方法评估了 FABP4、GINS2 和 CBX7 的表达。我们评估了它们的表达与临床病理和预后参数之间的关联:结果:发现FABP4和GINS2的高表达以及CBX7的低表达与高龄、肿瘤体积大、肿瘤分级高、淋巴管受累、宫旁器官浸润、FIGO分期晚期、化疗耐药和肿瘤复发呈正相关:我们证实了 FABP4 和 GISN2 在宫颈癌中的致癌作用,以及 CBX7 的协同抑制作用,并证实了它们与不良临床病理学标准和不良生存率之间的关联。我们的研究结果可能表明,FABP4、GISN2 和 CBX7 可被视为宫颈癌术前发生淋巴结转移的预测性生物标志物,有利于术前准确设计治疗方案。
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引用次数: 0
Primary Undifferentiated Pleomorphic Sarcoma of the Breast: A Case Report with Literature Review of Similar Cases. 乳腺原发性未分化多形性肉瘤:病例报告及类似病例文献综述。
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2023-12-29 DOI: 10.30699/IJP.2023.2006411.3139
Amirhossein Jafarian, Mohaddeseh Shahraki, Samaneh Sajjadi, Behrooz Daneshmand

Breast sarcoma is a rare but aggressive tumor. There are few case reports in the literature and several aspects of this disease are still not completely comprehended. Therefore, reporting new cases can help to enrich the literature. We report a patient with breast mass and pus secretion from her right breast, misdiagnosed as an abscess and mistreated by antibiotics. The patient was referred for an ultrasound examination and mammography, and a needle biopsy was performed that suggested an aggressive tumor. By the pathologist's suggestion, a total mastectomy of the right breast was performed with the excision of sentinel nodes. A pathological examination revealed a high-grade undifferentiated pleomorphic sarcoma (UPS) without vascular or lymph node invasion as the final diagnosis. The patient underwent postoperative chemotherapy and is currently in good condition. This case emphasizes considering this rare tumor when approaching a breast mass. Performing surgery with adequate resection margin can improve the patient's prognosis. Some suggested breast UPS cases with lung and brain metastasis would be more aggressive tumors than other breast sarcomas. Total mastectomy with negative margins and free-of-tumor lymph nodes may be the key to improve prognosis in such patients.

乳腺肉瘤是一种罕见的侵袭性肿瘤。文献中的病例报道很少,人们对这种疾病的一些方面仍未完全了解。因此,报告新病例有助于丰富文献。我们报告了一名乳房肿块和右侧乳房脓性分泌物的患者,她被误诊为脓肿,并被错误地使用抗生素治疗。患者被转诊接受超声波检查和乳房 X 光检查,并进行了针刺活检,结果显示是侵袭性肿瘤。根据病理学家的建议,患者接受了右侧乳房全切除术,并切除了前哨结节。病理检查显示,最终诊断为高级别未分化多形性肉瘤(UPS),无血管或淋巴结侵犯。患者接受了术后化疗,目前情况良好。该病例强调,在处理乳房肿块时应考虑到这种罕见肿瘤。手术切除足够的切缘可以改善患者的预后。有些人认为,与其他乳腺肉瘤相比,有肺转移和脑转移的乳腺 UPS 肿瘤更具侵袭性。边缘阴性和肿瘤淋巴结游离的全乳切除术可能是改善此类患者预后的关键。
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引用次数: 0
Strategic Framework for Strengthening Health Laboratory Services in the COVID-19 Pandemic in Iran. 在伊朗 COVID-19 大流行期间加强卫生实验室服务的战略框架》(Strategic Framework for Strengthening Health Laboratory Services in the COVID-19 Pandemic in Iran)。
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30699/IJP.2024.2013702.3192
Nooshafarin Safadel, Parisa Dahim, Siamak Mirab Samiee, Katayoon Khodaverdian, Maryam Mir Mohammad Ali Roodaki, Soghra Anjarani, Shahla Farsi, Mahdi Rafiee, Marjan R Farzami

Background & objective: Providing equitable access to good quality, timely, and affordable laboratory testing has always been a top priority for the Ministry of Health and Medical Education (MoH-ME) and the Reference Health Laboratory (RHL). Considering the significant role of medical laboratories in disease surveillance, RHL developed a strategic plan to manage laboratory services during the COVID-19 pandemic based on the "Strategic Framework for strengthening health laboratory services, 2016-2020" proposed by the World Health Organization (WHO). This article describes the steps taken to establish the strategic framework in Iran.

Methods: Firstly, a National Laboratory Committee was formed in MoH-ME and a situation analysis was conducted to explore the strengths, weaknesses, opportunities, and threats in different components of our laboratory system. Gaps and resources needed to address those gaps were determined; then, RHL outlined operational processes and mechanisms for monitoring the activities.

Results: The WHO strategic roadmap and its six strategic goals concerning leadership, quality, human resources, safety and security, laboratory networking, and rational use of laboratory testing, helped us to promote national laboratory services in accordance with health system requirements in the COVID-19 pandemic.

Conclusion: The establishment of a national molecular laboratory network with more than 500 laboratories from different sectors may result in timely access to countrywide laboratory services and would be beneficial for future COVID-19 and/or other viral outbreaks. Continual evaluation of the COVID-19 laboratories' performance, production of PCR test kits by the local manufacturers, and development of a platform for virtual training would be other accomplishments that Iran achieved in coping with the recent pandemic.

背景与目标:卫生和医学教育部(MoH-ME)和参考健康实验室(RHL)一直将公平提供优质、及时和负担得起的实验室检测服务作为首要任务。考虑到医学实验室在疾病监测中的重要作用,RHL 根据世界卫生组织(WHO)提出的 "2016-2020 年加强卫生实验室服务战略框架 "制定了一项战略计划,以便在 COVID-19 大流行期间管理实验室服务。本文介绍了伊朗为建立该战略框架所采取的步骤:首先,卫生部-教育部成立了国家实验室委员会,并进行了形势分析,以探讨实验室系统不同组成部分的优势、劣势、机遇和威胁。确定了差距和弥补这些差距所需的资源;然后,RHL 概述了监测活动的操作流程和机制:结果:世卫组织的战略路线图及其关于领导力、质量、人力资源、安全和安保、实验室网络和合理使用实验室检测的六个战略目标,帮助我们在 COVID-19 大流行期间按照卫生系统的要求促进了国家实验室服务:结论:建立一个由来自不同部门的 500 多个实验室组成的国家分子实验室网络,可使人们及时获得全国范围内的实验室服务,并有利于应对未来的 COVID-19 和/或其他病毒爆发。持续评估 COVID-19 实验室的绩效、由本地制造商生产 PCR 检测试剂盒以及开发虚拟培训平台,将是伊朗在应对近期大流行病方面取得的其他成就。
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引用次数: 0
Evaluation of Mir-1290 as a Possible Diagnostic Factor in the Serum of Oral Squamous Cell Carcinoma Patients with Qualitative Real-Time Polymerase Chain Reaction. 用定性实时聚合酶链反应评估口腔鳞状细胞癌患者血清中 Mir-1290 的可能诊断因子
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-02-15 DOI: 10.30699/IJP.2024.2010130.3161
Siavash Bagheri Shirvan, Mehdi Shahabinejad, Farnaz Mohajertehran, Nazanin Nazari

Background & objective: This study aimed to determine the incidence of microRNA (miRNA; miR-1290) in the serum of oral squamous cell carcinoma (OSCC) patients compared to a control group using the qualitative real-time polymerase chain reaction (PCR) method.

Methods: Blood serum samples were obtained from patients diagnosed with OSCC and confirmed through biopsy. The samples were collected from patients referred to the Mashhad Dental Faculty and Ghaem Hospital. The OSCC group consisted of 17 patients, while the healthy group included 15 individuals. RNA was extracted from the patient samples, and samples with an A260/280 ratio between 1.8 and 2.0 (indicating acceptable RNA quality) were immediately converted into complementary DNA (cDNA) using albumin and cDNA reference genes. The SYBR green real-time reverse transcriptase PCR method was used to measure the presence of miR-1290 in the blood samples.

Results: A total of 32 patients were examined in this study, including 17 women (53.1%) and 15 men (46.9%). The mean age was 46.7 years in the healthy group and 54.6 years in the SCC group, indicating a significant difference (P<0.05). The expression level of the miR-1290 gene was higher in patients with SCC compared to the healthy group (P=0.000). While the expression level of miR-1290 was higher in grade 3 and advanced stage than in grades 2 and 1 and early stage, the differences were not statistically significant (P=0.173 and P=0.564 for grade and stage, respectively).

Conclusion: The expression level of miR-1290 may increase in SCC patients compared to healthy individuals, making it a potential circulating biomarker. Further investigations for diagnostic utility would be warranted.

背景与目的:本研究旨在采用定性实时聚合酶链反应(PCR)方法,测定与对照组相比,口腔鳞状细胞癌(OSCC)患者血清中微RNA(miRNA;miR-1290)的发生率:方法:从确诊为口腔鳞状细胞癌(OSCC)并通过活检确诊的患者中采集血清样本。样本采集自转诊至马什哈德牙科学院和盖姆医院的患者。OSCC 组包括 17 名患者,健康组包括 15 人。从患者样本中提取 RNA,A260/280 比值在 1.8 和 2.0 之间(表明 RNA 质量合格)的样本立即使用白蛋白和 cDNA 参考基因转换成互补 DNA(cDNA)。采用 SYBR 绿色实时逆转录酶 PCR 法检测血液样本中 miR-1290 的含量:本研究共检查了 32 例患者,其中女性 17 例(53.1%),男性 15 例(46.9%)。健康组的平均年龄为 46.7 岁,SCC 组的平均年龄为 54.6 岁,差异显著(PP=0.000)。虽然 miR-1290 在 3 级和晚期的表达水平高于 2 级、1 级和早期,但差异无统计学意义(等级和分期分别为 P=0.173 和 P=0.564):结论:与健康人相比,miR-1290 在 SCC 患者中的表达水平可能会升高,使其成为一种潜在的循环生物标志物。结论:与健康人相比,miR-1290 在 SCC 患者中的表达水平可能会升高,这使其成为一种潜在的循环生物标志物。
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引用次数: 0
Cerebrospinal Fluid Analysis in Patients with Post-neurosurgical Procedures: Meningitis vs. Non-meningitis. 神经外科手术后患者脑脊液分析:脑膜炎与非脑膜炎
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.30699/IJP.2023.2019741.3240
Mehdi Zeinalizadeh, Maryam Shadkam, Pegah Afarinesh Khaki, Alireza Abdollahi, Masoumeh Douraghi, Mohammadreza Salehi

Background & objective: Cerebrospinal fluid (CSF) analysis is helpful in the diagnosis of infections of the central nervous system (CNS), especially after neurosurgical procedures. This study aimed to evaluate the diagnostic value of CSF markers for diagnosis of post-neurosurgical meningitis (PNM).

Methods: Patients with neurosurgical procedures whose CSF was obtained for any reason (meningitis and non-meningitis) during 2020 and 2022, at Imam Khomeini Hospital Complex, Tehran, Iran, were included. Serum and CSF lactate dehydrogenase (LDH), glucose, protein, white blood cells (WBC), red blood cells (RBC), and CSF/serum glucose and LDH ratio were compared between the patients who were diagnosed with PNM and those without meningitis.

Results: A total of 115 patients were included, of whom 23 patients were diagnosed with PNM and 92 with non-meningitis. No significant differences were observed in patients' age, gender, and underlying diseases between the two groups. Findings showed a significantly (P=0.029) lower level of the mean CSF glucose (59.5 mg/dL ±33.9) in patients with meningitis than in patients without meningitis (76.8 mg/dL ± 37.5). The mean CSF/serum glucose ratio was 43.7% in the meningitis group and 56.3% in the non-meningitis group (P=0.008). The mean WBC count and neutrophil dominance were significantly higher in the meningitis group. No significant differences were observed in CSF LDH, Protein, and RBC between the two groups.

Conclusion: A CSF glucose level of less than 60 mg/dL, a CSF/serum glucose ratio of less than 0.44, and a higher CSF WBC and neutrophil count can help diagnose PNM.

背景与目的:脑脊液(CSF)分析有助于中枢神经系统(CNS)感染的诊断,特别是神经外科手术后。本研究旨在评价脑脊液标志物对术后神经外科脑膜炎(PNM)的诊断价值。方法:纳入2020年至2022年期间在伊朗德黑兰伊玛目霍梅尼综合医院因任何原因(脑膜炎和非脑膜炎)获得脑脊液的神经外科手术患者。比较PNM与非脑膜炎患者血清和脑脊液乳酸脱氢酶(LDH)、葡萄糖、蛋白、白细胞(WBC)、红细胞(RBC)及脑脊液/血清葡萄糖和LDH比值。结果:共纳入115例患者,其中23例诊断为PNM, 92例诊断为非脑膜炎。两组患者的年龄、性别和基础疾病无显著差异。结果显示,脑膜炎患者脑脊液平均葡萄糖水平(59.5 mg/dL±33.9)明显低于非脑膜炎患者(76.8 mg/dL±37.5)。脑膜炎组脑脊液/血清葡萄糖平均比值为43.7%,非脑膜炎组为56.3% (P=0.008)。平均白细胞计数和中性粒细胞优势明显高于脑膜炎组。两组患者CSF LDH、蛋白、RBC均无显著差异。结论:脑脊液葡萄糖水平小于60 mg/dL,脑脊液/血清葡萄糖比值小于0.44,脑脊液白细胞和中性粒细胞计数较高有助于诊断PNM。
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引用次数: 0
Clinicopathological Spectrum of Diabetic and Non-diabetic Renal Lesions in Patients with Diabetes Mellitus: An Experience from A Tertiary Care Center. 糖尿病患者的糖尿病和非糖尿病肾病的临床病理谱:来自三级保健中心的经验。
Q3 Medicine Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.30699/IJP.2024.2024462.3270
Netra Prakash Kori, Manjunath Revanasiddappa, Nagraj D Naik, Atul Desai, Ranjana Shashidhar Ranade

Background & objective: Diabetic patients often develop lesions called non-diabetic renal diseases (NDRD), whose prognostic and therapeutic implications vary from diabetic nephropathy (DN). Since early identification of NDRD is associated with a better prognosis, we aimed to understand its spectrum.

Methods: One hundred and thirty-four patients were included in a cross-sectional study. Their clinical, and laboratory data and indications for biopsy were recorded. Two cores of renal tissue were studied under light microscopy and immunofluorescence; patients were classified into NDRD, NDRD+DN, and DN groups.

Results: Of all the patients studied, five were diagnosed with type 1 diabetes mellitus (DM1), and the rest were type 2 diabetes mellitus (DM2). Overall, the male-to-female ratio was 3:1. The Mean age of NDRD patients was the greatest, and males were predominant in all three groups. CKD was the most common presentation. Except for significantly greater proteinuria and hematuria in the DN and NDRD groups respectively, others were not different. Infection-related glomerulonephritis was the most common lesion among the NDRD+DN group, whereas IgA nephropathy and acute tubulointerstitial nephritis were frequent in the NDRD group.

Conclusion: Based on our findings, renal biopsy should be considered in all those diabetic patients with lesser onset duration presenting with hematuria, no/minimal retinopathy, and minimal proteinuria. A precise diagnosis helps in providing timely therapy for NDRD and prolonging renal and patient survival.

背景与目的:糖尿病患者经常发生非糖尿病性肾病(NDRD),其预后和治疗意义与糖尿病肾病(DN)不同。由于NDRD的早期识别与较好的预后相关,因此我们旨在了解其谱系。方法:对134例患者进行横断面研究。记录他们的临床、实验室数据和活检指征。在光镜和免疫荧光下对两个肾核组织进行了研究;将患者分为NDRD组、NDRD+DN组和DN组。结果:所有患者中,5例诊断为1型糖尿病(DM1),其余为2型糖尿病(DM2)。总体而言,男女比例为3:1。NDRD患者的平均年龄最大,且三组均以男性为主。CKD是最常见的表现。除了DN组和NDRD组的蛋白尿和血尿显著增加外,其他组无显著差异。感染相关性肾小球肾炎是NDRD+DN组中最常见的病变,而IgA肾病和急性小管间质性肾炎在NDRD组中常见。结论:根据我们的研究结果,对于所有发病时间较短且有血尿、无/轻微视网膜病变和轻微蛋白尿的糖尿病患者,应考虑肾活检。准确的诊断有助于为NDRD提供及时的治疗,延长肾脏和患者的生存。
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Iranian Journal of Pathology
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