An association between connective tissue growth factor (CTGF) gene dimorphism at -945 (CTGF*-945C/G) and systemic sclerosis (SSc) has been reported with inconclusive results. We performed this study to determine whether such an association exists among Thai patients with SSc. DNA samples were taken from 50 Thai SSc patients (diffuse SSc in 39 and limited SSc in 11) and 99 healthy controls for determination of CTGF*-945C/G dimorphism by polymerase chain reaction (PCR) using specific oligonucleotide primers. The associations between the genotype frequencies, clinical manifestations and auto-antibodies were determined as well. When compared with the controls, SSc patients had no significantly higher frequencies of the GG genotype (44.0% vs 39.4%, p = 0.60), G allele (63.0% vs 65.2%, p = 0.80) or G phenotype (82.0% vs 90.9%, p = 1.0). There was no association between the presence of the GG genotype and clinical manifestations (pulmonary fibrosis, sclerodactyly, digital pitting scars, telangiectasia and pulmonary arterial hypertension), or the presence of auto-antibodies (anti-Scl-70, anti-SSA/Ro, and anti-RNP). In conclusion, we found no association between CTGF*-945C/G dimorphism and Thai SSc patients.
结缔组织生长因子(CTGF)基因-945位点二态性(CTGF*-945C/G)与系统性硬化症(SSc)之间的关联已被报道，但结果尚无定论。我们进行这项研究是为了确定泰国SSc患者中是否存在这种关联。从50例泰国SSc患者(弥漫性SSc 39例，限制性SSc 11例)和99例健康对照中提取DNA样本，采用特异性寡核苷酸引物聚合酶链反应(PCR)检测CTGF*-945C/G二态性。基因型频率、临床表现和自身抗体之间的关系也被确定。与对照组相比，SSc患者GG基因型(44.0% vs 39.4%， p = 0.60)、G等位基因(63.0% vs 65.2%， p = 0.80)或G表型(82.0% vs 90.9%， p = 1.0)的频率均无显著性差异。GG基因型的存在与临床表现(肺纤维化、硬化、指痕、毛细血管扩张和肺动脉高压)或自身抗体(抗scl -70、抗ssa /Ro和抗rnp)的存在没有关联。总之，我们发现CTGF*-945C/G二态性与泰国SSc患者之间没有关联。
{"title":"Lack of CTGF*-945C/G Dimorphism in Thai Patients with Systemic Sclerosis.","authors":"Worawit Louthrenoo, Nuntana Kasitanon, Ramjai Wichainun, Suparaporn Wangkaew, Waraporn Sukitawut, Yuka Ohnogi, Shoji Kuwata, Fujio Takeuchi","doi":"10.2174/1874312901105010059","DOIUrl":"https://doi.org/10.2174/1874312901105010059","url":null,"abstract":"<p><p>An association between connective tissue growth factor (CTGF) gene dimorphism at -945 (CTGF*-945C/G) and systemic sclerosis (SSc) has been reported with inconclusive results. We performed this study to determine whether such an association exists among Thai patients with SSc. DNA samples were taken from 50 Thai SSc patients (diffuse SSc in 39 and limited SSc in 11) and 99 healthy controls for determination of CTGF*-945C/G dimorphism by polymerase chain reaction (PCR) using specific oligonucleotide primers. The associations between the genotype frequencies, clinical manifestations and auto-antibodies were determined as well. When compared with the controls, SSc patients had no significantly higher frequencies of the GG genotype (44.0% vs 39.4%, p = 0.60), G allele (63.0% vs 65.2%, p = 0.80) or G phenotype (82.0% vs 90.9%, p = 1.0). There was no association between the presence of the GG genotype and clinical manifestations (pulmonary fibrosis, sclerodactyly, digital pitting scars, telangiectasia and pulmonary arterial hypertension), or the presence of auto-antibodies (anti-Scl-70, anti-SSA/Ro, and anti-RNP). In conclusion, we found no association between CTGF*-945C/G dimorphism and Thai SSc patients.</p>","PeriodicalId":39124,"journal":{"name":"Open Rheumatology Journal","volume":" ","pages":"59-63"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/e4/TORJ-5-59.PMC3204428.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40123701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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