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The basic performance and field application test of newly developed flower thinning system for peach cultivation 新研制的桃栽培疏花系统基本性能及田间应用试验
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.17
Young Sik Park, Sangjun Im, J. Heo
We developed a pulsatory water spray gun system aimed at improving the efficiency of fruit setting management in peach cultivation and evaluated its feasibility for field application. In order to select the optimum water spray conditions and developmental stages of a flower for field applications, the influence of varying pressures of the power sprayer, nozzle diameters, and spray distances on flower and leaf bud thinning rate was investigated under four different developmental stages of flower such as bud sprouting, pink, balloon and flowering period using the water cut bearing branch of “Kawanakawase Hakuto” peach. The optimum conditions for the application of the pulsatory water spray gun were identified as a power sprayer pressure of 2 MPa at a nozzle diameter of 2 mm and spraying distance of 1 m in the ballooning and flowering stages. A flower bud thinning rate greater than 60 % and a leaf bud thinning rate lower than 7 % were observed under these conditions. In the field application test based on the selected conditions, the flower and leaf bud thinning rates were ideal for fruit production and showed higher fruit characteristics compared with fruits produced by a conventional fruit setting management method. In addition, the application of this system for flower thinning could considerably reduce the total work time compared with the conventional method. Hence, it is also expected that the newly developed system can be used for labor savings during fruit setting management in peach cultivation.
为了提高桃栽培坐果管理的效率,我们开发了一种脉动式喷水枪系统,并对其在田间应用的可行性进行了评估。为了选择田间应用的最佳喷水条件和花朵发育阶段,研究了在花蕾发芽、粉红色、,气球和花期使用“川川川白”桃的含水枝条。在鼓泡期和开花期,确定了应用脉动水喷枪的最佳条件是在喷嘴直径为2mm、喷射距离为1m的情况下,功率喷射器压力为2MPa。在这些条件下观察到大于60%的花蕾稀疏率和低于7%的叶芽稀疏率。在基于所选条件的田间应用试验中,与传统坐果管理方法生产的果实相比,花和叶芽稀疏率对果实生产来说是理想的,并且表现出更高的果实特性。此外,与传统方法相比,该系统在花卉间伐中的应用可以显著减少总工作时间。因此,新开发的系统也有望用于桃栽培坐果管理中的劳动力节约。
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引用次数: 0
Potential of Moroccan dates of Jihel variety 吉赫尔品种摩洛哥枣的潜力
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.22
O. Abba, A. Rochdi
Morocco’s phœnicicultural heritage is known for its varietal diversity. The present study aims to determine the potential of the Jihel variety of dates, compared to the other most economically important varieties of dates in Morocco. Based on a bibliographic analysis, a follow-up of units of dates and an analysis of the surveys were realized along with field data recovery. The present study shows that dates of Jihel variety have many criteria that permit its position as an important commercial produce. However, many interesting measures must be envisaged, in particular, the respect of the good practices of production, the accompaniment of the phoeniculturists, the encouragement of industrialists and the promotion of the dates of Jihel variety by the implementation of an appropriate communication approach: advertising campaigns, innovative concepts of packaging and labeling. The results represent an exploitable base to establish a roadmap, aiming for the development of the phoenicultural sector in particular dates of Jihel variety of Morocco.
摩洛哥的园艺遗产以其品种多样性而闻名。本研究旨在确定与摩洛哥其他经济上最重要的椰枣品种相比,Jihel品种椰枣的潜力。在书目分析的基础上,对日期单位进行了跟踪,并对调查进行了分析,同时恢复了实地数据。目前的研究表明,济和品种的椰枣有许多标准可以使其成为一种重要的商业产品。然而,必须设想许多有趣的措施,特别是尊重良好的生产做法,在养鱼者的陪同下,鼓励实业家,并通过实施适当的沟通方法来推广Jihel品种的日期:广告宣传、包装和标签的创新概念。研究结果为制定路线图提供了一个可利用的基础,旨在发展凤凰文化部门,尤其是摩洛哥的Jihel品种。
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引用次数: 1
Characterization of essential oil from the peel of three citrus species grown in Sikkim Himalaya 锡金-喜马拉雅三种柑橘果皮精油的性质
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.27
A. Pradhan, L. Sharma, S. Bhutia, N. Sherpa
The fruit peel of citrus is known to be rich in essential oil. Essential oil content and its characteristics vary amongst the species and climatic factors play a major role. Essential oil, to be suitable for the specific commercial purpose should have basic characteristics. In the present study, extracted oil of selected three citrus species Citrus reticulata, C. maxima and C. jambhiri grown at different altitude of Sikkim Himalaya were characterized. The oil yield was up to 2.4 % in mature fruits and 2.5 % in immature fruits at an altitude of > 1600 m in mandarin orange with significant variations concerning species and altitude. The various analysed oil parameters and their highest values in the study are refractive index (1.49), specific gravity (0.87), free fatty acid (7.83 %), acid value (8.37 %), saponification (163.37 mg KOH / g of oil), iodine value (116 g I2/100 g), peroxide value (36.33 meq O2/ kg), thiocyanogen (77.29 %), ester value (57.63 mg KOH/g), phenol (81.92 mg/g). These parameters also showed variations with altitude and the species. The C. reticulata grown at higher altitude (> 1600 m from MSL) was found to have better essential oils than C. maxima and C. jambhiri with some exceptions. The characteristics highly endorsed the use of these essential oils for food and cosmetic industries. The waste management with simultaneous production of valuable compound is the major perspective sought in the study.
众所周知,柑橘的果皮含有丰富的精油。不同种类的植物精油含量及其特性各不相同,气候因素起主要作用。精油,要适合于特定的商业用途,应该具备基本的特性。以锡金喜马拉雅地区不同海拔生长的柑橘(citrus reticulata, C. maxima, C. jambhiri)为研究对象,对其提取油进行了表征。在海拔100 ~ 1600 m处,柑桔成熟果实产油率达2.4%,未成熟果实产油率达2.5%,品种和海拔差异显著。所分析的各种油参数及其在研究中的最高值为折射率(1.49)、比重(0.87)、游离脂肪酸(7.83%)、酸值(8.37%)、皂化(163.37 mg KOH/g油)、碘值(116 g I2/100 g)、过氧化值(36.33 meq O2/ kg)、硫氰原(77.29%)、酯值(57.63 mg KOH/g)、苯酚(81.92 mg/g)。这些参数也随海拔和树种的变化而变化。生长在海拔较高(距MSL约1600 m)的网纹金缕草精油含量高于大黄金缕草和jambhiri金缕草,但也有例外。这些特性高度认可了这些精油在食品和化妆品行业的使用。同时生产有价化合物的废物处理是本研究寻求的主要方向。
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引用次数: 3
Weed control and response of transplanted uhaloa in the presence of two pre-emergence herbicides applied at the time of planting 在播种时施用两种出苗前除草剂的情况下,移栽白桦的杂草控制和反应
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.18
Scott B. Lukas, J. Defrank, Orville C. Baldos, R. Qin
The Hawaii Statewide Noxious Invasive Pest Program (SNIPP) and associated storm water management plans provide statutory justification for increased use of native plants along the State of Hawaii transportation corridors. The demand for native plants exceeds the availability of plant materials or seed. To produce seed stock and ensure seed lot purity, establishment protocols for weed control must first be defined. Uhaloa (Waltheria indica), a native Hawaiian broadleaf shrub has been identified for increased roadside usage, thus will be the focus of this research. Weed control during the establishment phase of uhaloa is essential for optimising establishment success. In this study, the efficacy and phytotoxicity of the pre-emergence herbicides oxadiazon and indaziflam, applied over uhaloa transplants, were evaluated. Crop and weed response to granular oxadiazon at 2.24 kg ai ha-1 and 4.48 kg ai ha-1 and flowable indaziflam at 24 g ai ha-1 and 49 g ai ha-1 were determined. Unacceptable uhaloa injury with both rates of indaziflam was recorded. Oxadiazon provided excellent broad-spectrum weed control with acceptable injury to uhaloa.
夏威夷州范围内的有害入侵害虫计划(SNIPP)和相关的雨水管理计划为夏威夷州交通走廊沿线增加本土植物的使用提供了法定理由。对本土植物的需求超过了植物材料或种子的可用性。为了生产种子库存并确保种子批次的纯度,必须首先确定杂草控制的建立协议。Uhaloa(Waltheria indica)是一种夏威夷本土阔叶灌木,已被确定为路边使用量增加,因此将成为本研究的重点。乌哈洛阿建立阶段的杂草控制对于优化建立成功至关重要。在这项研究中,评估了羽化前除草剂恶二唑和吲唑福明在uhaloa移植中的药效和植物毒性。测定了作物和杂草对2.24 kg ai ha-1和4.48 kg ai ha-的颗粒恶二唑和24 g ai ha-1、49 g ai ha-可流动吲唑仑的反应。记录了不可接受的uhaloa损伤和吲唑仑的两种发生率。奥沙地松提供了极好的广谱杂草控制,对乌哈洛亚的伤害可接受。
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引用次数: 0
Relationship of turmeric and tamarind leaf extract ratio with induction time and antioxidant activity synergism 姜黄和罗望子叶提取物比例与诱导时间和抗氧化活性协同作用的关系
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.24
S. Mulyani, B. A. Harsojuwono
This study aims to determine the ratio of turmeric rhizome extract (TE) and tamarind leaf extract (TLE), which is suitable for the time of induction and the highest antioxidant synergism. The TE:TLE ratio examined was 10:1; 10:1.5 and 10:2. The chosen variable was the amount of peroxide. Observations carried out for 7 hours at one-hour intervals using ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. Relationship between the time and absorbance were depicted to show the induction time and synergism in antioxidants. The results showed that all TE and TLE ratios had antioxidants that inhibited lipid oxidation reactions at the stage of initiation and propagation. TE and TLE ratios 10:1, 10:1.5 and 10:2 had induction time, respectively 2.9577; 3.0206 and 3.1882 hours with FTC method, 3.6116; 3.706 and 3.8722 h with the TBA method. Synergism in antioxidants was 103.534; 106.924 and 110.705 % with the FTC method, and 102.9393; 109.522 and 115.969 % with TBA. The highest antioxidant synergism in both methods was shown by the ratio of TE:TLE = 10:2. Key word: Turmeric, tamarind leaves, induction time, synergism antioxidant
本研究旨在确定姜黄根茎提取物(TE)和罗望子叶提取物(TLE)的比例,该比例适合诱导时间和最高的抗氧化协同作用。TE与TLE的比值为10:1;10:1.5和10:2。选择的变量是过氧化物的量。使用硫氰酸铁(FTC)法和硫代巴比妥酸(TBA)法每隔一小时进行7小时的观察。时间和吸光度之间的关系显示了抗氧化剂的诱导时间和协同作用。结果表明,所有TE和TLE比例在起始和繁殖阶段都具有抑制脂质氧化反应的抗氧化剂。TE和TLE比值分别为10:1、10:1.5和10:2,诱导时间分别为2.9577;FTC法分别为3.0206和3.1882小时,3.6116;3.706和3.8722小时。抗氧化剂的协同作用为103.534;FTC法分别为106.924和110.705%,102.9393;TBA分别为109.522和115.969%。两种方法中抗氧化协同作用最高的是TE:TLE=10:2。关键词:姜黄、罗望子叶、诱导时间、协同抗氧化
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引用次数: 1
Development of a new hybrid aquaponic system for increasing chilli production efficiency 提高辣椒生产效率的新型杂交水培系统的研制
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.26
T. Mester, G. Csakberenyi-Nagy, G. Turk, I. Lázár, T. Tóth
One of the concerns regarding aquaponic systems operating without conventional soil is the limited selection of plants available for cultivation. For a wider use of aquaponic systems increasing yields are inevitable. The aim of our research, started in 2017, was to eliminate the drawbacks of aquaponic systems using alternative solutions in order to remain in the framework of sustainable agriculture. In order to meet the above challenges, a hybrid, automated aquaponic system was installed in which the soil was not removed completely from the systems that are not completely closed as fish receive fresh water as well as keeping in this way hormones impeding growth at a low level. Crucians and African catfish were placed into two tanks of 7 m3. Further novelty of the system is the application of quail dung in a quantity determined specially for increasing the natural efficiency of the system. Three methods of cultivation were used and plant macroand micro-elements were measured. Results indicate that the system is suitable for growing different chilli types while quail dung input contributes to increase yield significantly therefore it can be integrated into aquaponic systems excellently.
关于在没有传统土壤的情况下运行的水培系统的一个问题是可供种植的植物选择有限。为了更广泛地使用水培系统,提高产量是不可避免的。我们的研究始于2017年,目的是利用替代解决方案消除水培系统的缺点,以保持在可持续农业的框架内。为了应对上述挑战,安装了一个混合的自动水培系统,在该系统中,由于鱼类接受淡水,土壤没有完全从没有完全封闭的系统中清除,并以这种方式将阻碍生长的激素保持在较低水平。鲫鱼和非洲鲶鱼被放入两个7立方米的水箱中。该系统的另一个新颖之处是应用鹌鹑粪便,其量是专门为提高系统的自然效率而确定的。采用三种栽培方法,测定了植物的宏观和微观元素。结果表明,该系统适合种植不同类型的辣椒,而鹌鹑粪的投入有助于显著提高产量,因此可以很好地整合到水培系统中。
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引用次数: 0
Bioactivity of plant extracts against tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) 植物提取物对番茄潜叶蛾的生物活性
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.25
A. Ndereyimana, S. Nyalala, Patrick Murerwa, S. Gaidashova
Tomato (Solanum lycopersicum L.) is economically and nutritionally important in Rwanda, but its production is challenged by the tomato leaf miner (Tuta absoluta Meyrick), an invasive pest. Synthetic insecticides which are primarily used for its control, have various drawbacks. Bioactivity of Tephrosia vogelii, Tithonia diversifolia, Vernonia amygdalina and Phytolacca dodecandra aqueous extracts was evaluated against T. absoluta in laboratory. Leaflets with third instar larvae (3.85 5.65 mm) of T. absoluta in mines were collected from established tomato field. Aqueous plants extracts were evaluated at a dose of 10 % weight/volume. Sterile tap water and azadirachtin 0.03 % EC were used as negative and positive controls, respectively. Petri-dishes of 9 cm diameter (n=10) were used as bioassay arenas in a completely randomized design with four replications. Data on larval mortality were collected every 24 h for 5 days. Three bioassays were conducted on different dates. Results indicated that tested plant extracts exhibited a capacity to kill T. absoluta larvae in tomato leaf galleries with significant difference among them (P <0.0001). The killing capacity increased with exposure time. At 24 h of exposure, T. absoluta larvae mortality was in a range of 35.0 37.5 % for azadirachtin and 5.0 10.0 % for T. vogelii while all other aqueous extracts had 0.0 % mortality, except V. amygdalina which recorded 2.5 % in bioassay one. In all bioassays, the lowest mortality recorded 5 days after treatments with T. vogelii, T. diversifolia, V. amygdalina, P. dodecandra and azadirachtin was 32.2, 2.8. 2.5, 20.5 and 97.5 % while the highest mortality at this time was 35.1, 10.6, 13.3, 24.9 and 100 %, respectively. Tephrosia vogelii and P. dodecandra, which recorded higher efficacy compared to the other local plants, should be advanced to field evaluation. The observed higher efficacy of azadirachtin to Rwandan population of T. absoluta should also be confirmed under field conditions.
番茄(Solanum lycopersicum L.)在卢旺达具有重要的经济和营养价值,但其生产受到入侵害虫番茄潜叶虫(Tuta absoluta Meyrick)的挑战。主要用于控制它的合成杀虫剂有各种缺点。采用室内实验方法,评价了虎皮草、山竹、苦杏仁Vernonia amygdalina和商陆水提取物对绝对T.absoluta的生物活性。从已建立的番茄田中采集了三龄绝对T.absoluta幼虫(3.85~5.65mm)的小叶。以10%重量/体积的剂量评价植物水提取物。无菌自来水和印楝素0.03%EC分别作为阴性和阳性对照。直径为9厘米(n=10)的培养皿作为生物测定场地,采用完全随机设计,四次重复。幼虫死亡率数据每24小时收集一次,持续5天。在不同的日期进行了三次生物测定。结果表明,试验植物提取物对番茄叶廊中的绝对T.absoluta幼虫具有一定的杀灭能力,两者之间差异有统计学意义(P<0.0001),且随着暴露时间的延长,其杀灭能力逐渐增强。在暴露24小时时,印楝素的绝对T.absoluta幼虫死亡率为35.0 37.5%,伏氏T.vogelii的死亡率为5.0 10.0%,而所有其他水提取物的死亡率为0.0%,除了在生物测定中记录了2.5%的苦杏仁藤。在所有的生物测定中,在用伏氏T.vogelii、多样叶T.diversifolia、苦杏V.amydalina、十二andra和印楝素处理后5天记录的最低死亡率为32.2,2.8。2.5、20.5和97.5%,而此时的最高死亡率分别为35.1、10.6、13.3、24.9和100%。与其他当地植物相比,五倍子和十二香的药效更高,应进行实地评估。观察到的印楝素对卢旺达绝对T.absoluta种群的更高效力也应在田间条件下得到证实。
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引用次数: 2
Tree used in horticulture based alley cropping 用于园艺小巷种植的树木
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.16
O. Montes, R. Castro, C. Villanueva, M. Pérez, M. Uribe
Main objective of this paper is selection of tree and vegetable combination for success in new horticulture based alley cropping plantations. In alley cropping, fruit, legume and wood tree are integrated in several associations with annual vegetable, Mangifera indica, Psidium guajava, Citrus spp., Leucaena spp., Gliricidia sepium, Cajanus cajan, Populus spp., Eucalyptus, Hevea brasiliensis are the main tree used. Fabaceae, Brassicaceae, Solanaceae families and tuber crops are the main associated vegetable crops. This system can be adapted for specific farmers, regions, and countries for vegetable, fruit and wood requirements. The specific credits and subsidies can increase natural, economic, social capital of the world.
本文的主要目的是果树与蔬菜组合的选择,以成功的新园艺为基础的小巷种植人工林。在旱地种植中,水果、豆科植物和乔木以一年生蔬菜为主,以芒果、瓜石榴、柑橘、银合欢、木合欢、Cajanus cajan、杨树、桉树、巴西橡胶树为主要树种。豆科、十字花科、茄科和块茎作物是主要的伴生蔬菜作物。该系统可以适应特定的农民、地区和国家对蔬菜、水果和木材的需求。具体的信贷和补贴可以增加世界的自然、经济和社会资本。
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引用次数: 2
Applications of vegetation indices and biostimulators to the rooting of camellia cuttings 植被指数和生物激励剂在山茶插条生根中的应用
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-08-15 DOI: 10.37855/jah.2019.v21i02.19
P. Hou, K. Lin, Yen-Jung Huang, Chun-Wei Wu, Yu-Sen Chang
There are no reports on biostimulators being used to stimulate the rooting process of camellia (Camellia japonica) cuttings. The influences of vegetation indices and biostimulators on the rooting of shoot cuttings of the camellia ‘Nine Bends’ were determined using the reflectance spectroscopy. Six root growth parameters were recorded, 81 days after sticking cuttings. Different groups of soil-plant analysis development (SPAD) and normalized difference vegetation index (NDVI) values were used. Higher SPAD and NDVI values were found to be most effective in predicting rooting stages and percentages, whereas root number and length and dry weight could not be predicted. Indole-3-butyric acid (IBA) at a concentration of 2000 ppm alone or combined with three concentrations of thiamine (TA), ascorbic acid (AA) and catechol (CAT) were applied to cuttings of camellia. The maximum root number and dry weight were recorded in TA (1000 mg L-1) alone and IBA+TA (800 mg L-1). However, IBA+AA (1000 mg L-1) treated cuttings showed the highest rooting stage, root number, root length, and dry weight compared to other treatments. Cuttings treated with IBA+CAT (50 or 100 mg L-1) produced greater root number, length, and dry weight compared to other treatments. The effects of biostimulators applied to tea stem cuttings for raising camellia plants in the nursery would offer insights into the mechanism of its action in plant conservation, and there is a need to improve propagation technology.
目前还没有关于生物激励器用于刺激山茶插条生根过程的报道。利用反射光谱法测定了植被指数和生物刺激剂对“九弯”山茶枝条生根的影响。在插穗后81天,记录了6个根系生长参数。使用不同组的土壤植物分析发展(SPAD)和归一化差异植被指数(NDVI)值。较高的SPAD和NDVI值在预测生根阶段和百分比方面最有效,而根数、长度和干重则无法预测。将浓度为2000ppm的吲哚-3-丁酸(IBA)单独或与三种浓度的硫胺素(TA)、抗坏血酸(AA)和邻苯二酚(CAT)组合应用于山茶花的插条。在单独的TA(1000mg L-1)和IBA+TA(800mg L-1。然而,与其他处理相比,IBA+AA(1000mg L-1)处理的插穗表现出最高的生根阶段、根数、根长和干重。与其他处理相比,用IBA+CAT(50或100 mg L-1)处理的插条产生了更大的根数、长度和干重。将生物激励器应用于茶树茎插条以在苗圃中饲养山茶植物的效果将为深入了解其在植物保护中的作用机制提供见解,并且有必要改进繁殖技术。
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引用次数: 0
In vitro cormlet production- an efficient means for conservation in Ensete superbum (Roxb.) Cheesman 体外球茎生产-一种有效的超级细菌(Roxb.)Cheesman保护方法
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-04-15 DOI: 10.37855/jah.2019.v21i01.03
T. G. Ponni, A. Nair
Ensete superbum from the family Musaceae is commonly known as Kallu vazha (wild/ rock/cliff banana). The species holds a precise position in the field of medicine for its anti-hyperglycemic, anti-diuretic and spermicidal potential as well as ornamental value in botanical gardens. Due to deforestation, habitat fragmentation, indiscriminate harvesting for commercial gain, absence of suckers, and recalcitrant nature of seeds; this species is facing a drastic reduction in its propagation. The present study developed a protocol for the production of cormlets from explants isolated from inflorescence. The explants were cultured on MS media supplemented with 4mg L-1 BAP and 1.5 mg L-1 KIN and an average of six to ten cormlets were produced/ explants within eight weeks. Shoot induction occurred from the cormlets on MS medium with 3mg L-1 IBA and 1.5 mg L-1 BAP. Cormlets inoculated on MS medium supplemented with 1000 mg L-1 glutamine for a period of four weeks enhanced the size of cormlets which in turn increased the number of shoots. An average of ten multiple shoots were obtained on MS medium supplemented with 5 mg L-1 BAP. Maximum rooting was obtained on half strength MS medium with 3 mg L-1 IBA, 0.1 mg L-1 BAP and 1% activated charcoal. The plantlets were transferred to Knop’s solution for acclimatization. Rooted plants were hardened successfully in cocopeat along with sand in 1:1 combination and transferred to soil with 98% survival rate.
木犀科的超级木犀通常被称为Kallu vazha(野生/岩石/悬崖香蕉)。该物种因其抗高血糖、抗利尿和杀精子的潜力以及在植物园中的观赏价值而在医学领域占据着精确的地位。由于森林砍伐、栖息地破碎化、为商业利益而不分青红皂白地收割、缺乏吸盘以及种子的顽固性;这个物种正面临着繁殖的急剧减少。本研究开发了一种从花序中分离的外植体生产球茎的方案。外植体在补充4mg L-1 BAP和1.5mg L-1 KIN的MS培养基上培养,在8周内平均产生6至10个球茎/外植体。球茎在含有3mg L-1 IBA和1.5mg L-1 BAP的MS培养基上进行芽诱导。球茎在补充有1000mg L-1谷氨酰胺的MS培养基上接种四周,可增强球茎的大小,进而增加芽的数量。在添加5mg L-1 BAP的MS培养基上平均获得10个多芽。在含有3mg L-1 IBA、0.1mg L-1 BAP和1%活性炭的半强度MS培养基上获得最大生根。将植株转移到Knop溶液中进行驯化。生根植物在椰子泥炭和沙子中以1:1的组合成功地硬化,并转移到土壤中,存活率为98%。
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引用次数: 3
期刊
Journal of Applied Horticulture
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